                         SEQUENCE LISTING

<110>  City of Hope
 
<120>  CD33 TARGETED CHIMERIC ANTIGEN RECEPTOR MODIFIED T CELLS FOR 
       TREATMENT OF CD33 POSITIVE MALIGNANCIES

<130>  40056-0052WO1

<140>  PCT/US2020/035579
<141>  2020-06-01

<150>  US 62/855,906
<151>  2019-05-31

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD33 scFv

<400>  1

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Asn Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Tyr Pro Tyr Asn Gly Gly Thr Gly Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Ser Lys Ala Thr Ile Thr Ala Asp Glu Ser Thr Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Arg Pro Ala Met Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
    130                 135                 140                 


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Ser Val 
145                 150                 155                 160 


Asp Asn Tyr Gly Ile Ser Phe Met Asn Trp Phe Gln Gln Lys Pro Gly 
                165                 170                 175     


Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Asn Gln Gly Ser Gly 
            180                 185                 190         


Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Thr Ile Ser Ser Leu Gln Pro Asp Asp Phe Ala Thr Tyr Tyr Cys Gln 
    210                 215                 220                 


Gln Ser Lys Glu Val Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu 
225                 230                 235                 240 


Ile Lys 
        


<210>  2
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  2

Gly Gly Gly Ser Ser Gly Gly Gly Ser Gly 
1               5                   10  


<210>  3
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG4 hinge (S to P) (S228P)

<400>  3

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10          


<210>  4
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG4 hinge

<400>  4

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro 
1               5                   10          


<210>  5
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG4 hinge (S228P)+ linker

<400>  5

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Gly Gly Gly Ser 
1               5                   10                  15      


Ser Gly Gly Gly Ser Gly 
            20          


<210>  6
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 hinge

<400>  6

Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn 
1               5                   10                  15      


Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 
            20                  25                  30          


Phe Pro Gly Pro Ser Lys Pro 
        35                  


<210>  7
<211>  48
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 hinge-48aa

<400>  7

Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
1               5                   10                  15      


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
            20                  25                  30          


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45              


<210>  8
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 hinge-45aa

<400>  8

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  9
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG4(HL-CH3) (includes S228P in hinge)

<400>  9

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Gly Gly Gly Ser 
1               5                   10                  15      


Ser Gly Gly Gly Ser Gly Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
            20                  25                  30          


Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr 
        35                  40                  45              


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
    50                  55                  60                  


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
65                  70                  75                  80  


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys 
                85                  90                  95      


Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
            100                 105                 110         


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly 
        115                 120                 125             


Lys 
    


<210>  10
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG4(L235E,N297Q)

<400>  10

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Gln Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  11
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG4(S228P, L235E,N297Q)

<400>  11

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Gln Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  12
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG4(CH3)

<400>  12

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu 
1               5                   10                  15      


Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
            20                  25                  30          


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
        35                  40                  45              


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
    50                  55                  60                  


Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly 
65                  70                  75                  80  


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
                85                  90                  95      


Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
            100                 105         


<210>  13
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3z

<400>  13

Leu Cys Tyr Leu Leu Asp Gly Ile Leu Phe Ile Tyr Gly Val Ile Leu 
1               5                   10                  15      


Thr Ala Leu Phe Leu 
            20      


<210>  14
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28

<400>  14

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  15
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28(M)

<400>  15

Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser 
1               5                   10                  15      


Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25              


<210>  16
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD4

<400>  16

Met Ala Leu Ile Val Leu Gly Gly Val Ala Gly Leu Leu Leu Phe Ile 
1               5                   10                  15      


Gly Leu Gly Ile Phe Phe 
            20          


<210>  17
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8tm

<400>  17

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr 
            20      


<210>  18
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8tm2

<400>  18

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr 
            20              


<210>  19
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8tm3

<400>  19

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  20
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  41BB

<400>  20

Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu 
1               5                   10                  15      


Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val 
            20                  25          


<210>  21
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3

<400>  21

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  22
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28

<400>  22

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  23
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28gg*

<400>  23

Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  24
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  41BB

<400>  24

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  25
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OX40

<400>  25

Ala Leu Tyr Leu Leu Arg Arg Asp Gln Arg Leu Pro Pro Asp Ala His 
1               5                   10                  15      


Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln 
            20                  25                  30          


Ala Asp Ala His Ser Thr Leu Ala Lys Ile 
        35                  40          


<210>  26
<211>  323
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated CD19R

<400>  26

Met Pro Pro Pro Arg Leu Leu Phe Phe Leu Leu Phe Leu Thr Pro Met 
1               5                   10                  15      


Glu Val Arg Pro Glu Glu Pro Leu Val Val Lys Val Glu Glu Gly Asp 
            20                  25                  30          


Asn Ala Val Leu Gln Cys Leu Lys Gly Thr Ser Asp Gly Pro Thr Gln 
        35                  40                  45              


Gln Leu Thr Trp Ser Arg Glu Ser Pro Leu Lys Pro Phe Leu Lys Leu 
    50                  55                  60                  


Ser Leu Gly Leu Pro Gly Leu Gly Ile His Met Arg Pro Leu Ala Ile 
65                  70                  75                  80  


Trp Leu Phe Ile Phe Asn Val Ser Gln Gln Met Gly Gly Phe Tyr Leu 
                85                  90                  95      


Cys Gln Pro Gly Pro Pro Ser Glu Lys Ala Trp Gln Pro Gly Trp Thr 
            100                 105                 110         


Val Asn Val Glu Gly Ser Gly Glu Leu Phe Arg Trp Asn Val Ser Asp 
        115                 120                 125             


Leu Gly Gly Leu Gly Cys Gly Leu Lys Asn Arg Ser Ser Glu Gly Pro 
    130                 135                 140                 


Ser Ser Pro Ser Gly Lys Leu Met Ser Pro Lys Leu Tyr Val Trp Ala 
145                 150                 155                 160 


Lys Asp Arg Pro Glu Ile Trp Glu Gly Glu Pro Pro Cys Val Pro Pro 
                165                 170                 175     


Arg Asp Ser Leu Asn Gln Ser Leu Ser Gln Asp Leu Thr Met Ala Pro 
            180                 185                 190         


Gly Ser Thr Leu Trp Leu Ser Cys Gly Val Pro Pro Asp Ser Val Ser 
        195                 200                 205             


Arg Gly Pro Leu Ser Trp Thr His Val His Pro Lys Gly Pro Lys Ser 
    210                 215                 220                 


Leu Leu Ser Leu Glu Leu Lys Asp Asp Arg Pro Ala Arg Asp Met Trp 
225                 230                 235                 240 


Val Met Glu Thr Gly Leu Leu Leu Pro Arg Ala Thr Ala Gln Asp Ala 
                245                 250                 255     


Gly Lys Tyr Tyr Cys His Arg Gly Asn Leu Thr Met Ser Phe His Leu 
            260                 265                 270         


Glu Ile Thr Ala Arg Pro Val Leu Trp His Trp Leu Leu Arg Thr Gly 
        275                 280                 285             


Gly Trp Lys Val Ser Ala Val Thr Leu Ala Tyr Leu Ile Phe Cys Leu 
    290                 295                 300                 


Cys Ser Leu Val Gly Ile Leu His Leu Gln Arg Ala Leu Val Leu Arg 
305                 310                 315                 320 


Arg Lys Arg 
            


<210>  27
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ribosomal skip sequence

<400>  27

Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp 
1               5                   10                  15      


Val Glu Glu Asn Pro Gly Pro Arg 
            20                  


<210>  28
<211>  354
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated EGFR

<400>  28

Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala Phe Leu 
1               5                   10                  15      


Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys 
            20                  25                  30          


Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys 
        35                  40                  45              


Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly 
    50                  55                  60                  


Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile 
65                  70                  75                  80  


Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp 
                85                  90                  95      


Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile 
            100                 105                 110         


Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser 
        115                 120                 125             


Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp 
    130                 135                 140                 


Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr 
145                 150                 155                 160 


Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile 
                165                 170                 175     


Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys 
            180                 185                 190         


His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp 
        195                 200                 205             


Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys 
    210                 215                 220                 


Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu 
225                 230                 235                 240 


Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr 
                245                 250                 255     


Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile 
            260                 265                 270         


Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu 
        275                 280                 285             


Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His 
    290                 295                 300                 


Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu 
305                 310                 315                 320 


Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met 
                325                 330                 335     


Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu 
            340                 345                 350         


Phe Met 
        


<210>  29

<400>  29
000

<210>  30
<211>  468
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD33 CD8 CAR

<400>  30

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Asn Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Tyr Pro Tyr Asn Gly Gly Thr Gly Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Ser Lys Ala Thr Ile Thr Ala Asp Glu Ser Thr Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Arg Pro Ala Met Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
    130                 135                 140                 


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Ser Val 
145                 150                 155                 160 


Asp Asn Tyr Gly Ile Ser Phe Met Asn Trp Phe Gln Gln Lys Pro Gly 
                165                 170                 175     


Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Asn Gln Gly Ser Gly 
            180                 185                 190         


Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Thr Ile Ser Ser Leu Gln Pro Asp Asp Phe Ala Thr Tyr Tyr Cys Gln 
    210                 215                 220                 


Gln Ser Lys Glu Val Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu 
225                 230                 235                 240 


Ile Lys Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
                245                 250                 255     


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
            260                 265                 270         


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
        275                 280                 285             


Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser 
    290                 295                 300                 


Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
305                 310                 315                 320 


Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
                325                 330                 335     


Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
            340                 345                 350         


Leu Gly Gly Gly Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
        355                 360                 365             


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
    370                 375                 380                 


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
385                 390                 395                 400 


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
                405                 410                 415     


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
            420                 425                 430         


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
        435                 440                 445             


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
    450                 455                 460                 


Leu Pro Pro Arg 
465             


<210>  31
<211>  650
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD33 EQ CAR

<400>  31

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Asn Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Tyr Pro Tyr Asn Gly Gly Thr Gly Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Ser Lys Ala Thr Ile Thr Ala Asp Glu Ser Thr Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Arg Pro Ala Met Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
    130                 135                 140                 


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Ser Val 
145                 150                 155                 160 


Asp Asn Tyr Gly Ile Ser Phe Met Asn Trp Phe Gln Gln Lys Pro Gly 
                165                 170                 175     


Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Asn Gln Gly Ser Gly 
            180                 185                 190         


Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Thr Ile Ser Ser Leu Gln Pro Asp Asp Phe Ala Thr Tyr Tyr Cys Gln 
    210                 215                 220                 


Gln Ser Lys Glu Val Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu 
225                 230                 235                 240 


Ile Lys Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro 
                245                 250                 255     


Glu Phe Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
            260                 265                 270         


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
        275                 280                 285             


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
    290                 295                 300                 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
305                 310                 315                 320 


Gln Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
                325                 330                 335     


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
            340                 345                 350         


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
        355                 360                 365             


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
    370                 375                 380                 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
385                 390                 395                 400 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
                405                 410                 415     


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
            420                 425                 430         


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
        435                 440                 445             


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    450                 455                 460                 


Leu Ser Leu Ser Leu Gly Lys Met Ala Leu Ile Val Leu Gly Gly Val 
465                 470                 475                 480 


Ala Gly Leu Leu Leu Phe Ile Gly Leu Gly Ile Phe Phe Lys Arg Gly 
                485                 490                 495     


Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
            500                 505                 510         


Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
        515                 520                 525             


Glu Glu Gly Gly Cys Glu Leu Gly Gly Gly Arg Val Lys Phe Ser Arg 
    530                 535                 540                 


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
545                 550                 555                 560 


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
                565                 570                 575     


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
            580                 585                 590         


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
        595                 600                 605             


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
    610                 615                 620                 


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
625                 630                 635                 640 


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                645                 650 


<210>  32
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD33 Vl

<400>  32

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Asn Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Tyr Pro Tyr Asn Gly Gly Thr Gly Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Ser Lys Ala Thr Ile Thr Ala Asp Glu Ser Thr Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Arg Pro Ala Met Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  33
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD33 Vh

<400>  33

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Met Ser Cys Lys Ser Ser Gln Ser Ile Leu Tyr Ser 
            20                  25                  30          


Ser Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys His Gln 
                85                  90                  95      


Tyr Leu Ser Ser Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  34
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GMCSFRa signal sequence

<400>  34

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro 
            20          


<210>  35
<211>  2616
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD33 CD8 CAR preceded by a GMCSR signal sequence and followed by 
       a T2A skip sequence and EGFRt

<400>  35
atgctgctcc ttgtcacatc cctgctgctg tgcgaactgc cacatcccgc cttcctgctg       60

atcccccaag tgcagctcgt gcagtccgga gccgaagtca agaagcctgg cagctccgtc      120

aaggtgtcct gcaaagcctc cggctacacc tttaccgact acaacatgca ctgggtccgc      180

caagcacctg gacagggact ggagtggatt gggtacatct acccttacaa cggaggcacc      240

gggtacaacc agaagttcaa gtcgaaggcc accattaccg cggacgaatc caccaacacc      300

gcgtatatgg agctctcatc cttgcggtcg gaggacactg ccgtgtacta ctgcgcgagg      360

ggtagaccgg caatggacta ctggggccag ggcactctcg tcaccgtgtc ctctggtggt      420

ggaggctcag gaggaggggg atccggtgga ggagggagcg atatccagat gacgcagtca      480

ccctcgtccc tgagcgcttc cgtgggcgat cgcgtgacta tcacttgccg ggcttccgag      540

tccgtggata actacggaat ttcctttatg aactggttcc agcaaaagcc gggaaaggcc      600

ccaaagctcc tgatctacgc cgccagcaat cagggatcgg gagtgccctc acggttctcc      660

gggagcggtt caggcaccga cttcaccctt actatttcga gcctgcaacc tgacgatttc      720

gccacttatt actgccaaca gtccaaggaa gtgccgtgga cgttcggcca ggggaccaag      780

gtggaaatca agaccacgac gccagcgccg cgaccaccaa caccggcgcc caccatcgcg      840

tcgcagcccc tgtccctgcg cccagaggcg tgccggccag cggcgggggg cgcagtgcac      900

acgagggggc tggacttcgc ctgtgatatc tacatctggg cgcccttggc cgggacttgt      960

ggggtccttc tcctgtcact ggttatcacc ctttactgca agcggggcag aaagaagctg     1020

ctgtacatct tcaagcagcc cttcatgcgg cccgtgcaga ccacccagga agaggacggc     1080

tgcagctgcc ggttccccga ggaagaggaa ggcggctgcg agctgggagg cggcagagtg     1140

aagttcagcc ggtccgccga cgcccctgcc taccagcagg gccagaacca gctgtacaac     1200

gagctgaacc tgggcaggcg ggaggaatac gacgtgctgg acaagcggag aggccgggac     1260

cctgagatgg gcggcaagcc caggcggaag aaccctcagg aaggcctgta taacgaactg     1320

cagaaagaca agatggccga ggcctacagc gagatcggca tgaagggcga gcggcggagg     1380

ggcaagggcc acgacggcct gtaccagggc ctgagcaccg ccaccaagga tacctacgac     1440

gccctgcaca tgcaggccct gcccccaagg ctcgagggcg gcggagaggg cagaggaagt     1500

cttctaacat gcggtgacgt ggaggagaat cccggcccta ggatgcttct cctggtgaca     1560

agccttctgc tctgtgagtt accacaccca gcattcctcc tgatcccacg caaagtgtgt     1620

aacggaatag gtattggtga atttaaagac tcactctcca taaatgctac gaatattaaa     1680

cacttcaaaa actgcacctc catcagtggc gatctccaca tcctgccggt ggcatttagg     1740

ggtgactcct tcacacatac tcctcctctg gatccacagg aactggatat tctgaaaacc     1800

gtaaaggaaa tcacagggtt tttgctgatt caggcttggc ctgaaaacag gacggacctc     1860

catgcctttg agaacctaga aatcatacgc ggcaggacca agcaacatgg tcagttttct     1920

cttgcagtcg tcagcctgaa cataacatcc ttgggattac gctccctcaa ggagataagt     1980

gatggagatg tgataatttc aggaaacaaa aatttgtgct atgcaaatac aataaactgg     2040

aaaaaactgt ttgggacctc cggtcagaaa accaaaatta taagcaacag aggtgaaaac     2100

agctgcaagg ccacaggcca ggtctgccat gccttgtgct cccccgaggg ctgctggggc     2160

ccggagccca gggactgcgt ctcttgccgg aatgtcagcc gaggcaggga atgcgtggac     2220

aagtgcaacc ttctggaggg tgagccaagg gagtttgtgg agaactctga gtgcatacag     2280

tgccacccag agtgcctgcc tcaggccatg aacatcacct gcacaggacg gggaccagac     2340

aactgtatcc agtgtgccca ctacattgac ggcccccact gcgtcaagac ctgcccggca     2400

ggagtcatgg gagaaaacaa caccctggtc tggaagtacg cagacgccgg ccatgtgtgc     2460

cacctgtgcc atccaaactg cacctacgga tgcactgggc caggtcttga aggctgtcca     2520

acgaatgggc ctaagatccc gtccatcgcc actgggatgg tgggggccct cctcttgctg     2580

ctggtggtgg ccctggggat cggcctcttc atgtga                               2616


<210>  36
<211>  871
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD33 CD8 CAR preceded by a GMCSR signal sequence and followed by 
       a T2A skip sequence and EGFRt

<400>  36

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu 
            20                  25                  30          


Val Lys Lys Pro Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Asp Tyr Asn Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Gln Gly Leu Glu Trp Ile Gly Tyr Ile Tyr Pro Tyr Asn Gly Gly Thr 
65                  70                  75                  80  


Gly Tyr Asn Gln Lys Phe Lys Ser Lys Ala Thr Ile Thr Ala Asp Glu 
                85                  90                  95      


Ser Thr Asn Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Gly Arg Pro Ala Met Asp Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser 
145                 150                 155                 160 


Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys 
                165                 170                 175     


Arg Ala Ser Glu Ser Val Asp Asn Tyr Gly Ile Ser Phe Met Asn Trp 
            180                 185                 190         


Phe Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala 
        195                 200                 205             


Ser Asn Gln Gly Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser 
    210                 215                 220                 


Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Asp Asp Phe 
225                 230                 235                 240 


Ala Thr Tyr Tyr Cys Gln Gln Ser Lys Glu Val Pro Trp Thr Phe Gly 
                245                 250                 255     


Gln Gly Thr Lys Val Glu Ile Lys Thr Thr Thr Pro Ala Pro Arg Pro 
            260                 265                 270         


Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro 
        275                 280                 285             


Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu 
    290                 295                 300                 


Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys 
305                 310                 315                 320 


Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly 
                325                 330                 335     


Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
            340                 345                 350         


Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
        355                 360                 365             


Glu Glu Gly Gly Cys Glu Leu Gly Gly Gly Arg Val Lys Phe Ser Arg 
    370                 375                 380                 


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
385                 390                 395                 400 


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
                405                 410                 415     


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
            420                 425                 430         


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
        435                 440                 445             


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
    450                 455                 460                 


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
465                 470                 475                 480 


Ala Leu His Met Gln Ala Leu Pro Pro Arg Leu Glu Gly Gly Gly Glu 
                485                 490                 495     


Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly 
            500                 505                 510         


Pro Arg Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro 
        515                 520                 525             


His Pro Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly 
    530                 535                 540                 


Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys 
545                 550                 555                 560 


His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro 
                565                 570                 575     


Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro 
            580                 585                 590         


Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu 
        595                 600                 605             


Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu 
    610                 615                 620                 


Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser 
625                 630                 635                 640 


Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu 
                645                 650                 655     


Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu 
            660                 665                 670         


Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly 
        675                 680                 685             


Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala 
    690                 695                 700                 


Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly 
705                 710                 715                 720 


Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg 
                725                 730                 735     


Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe 
            740                 745                 750         


Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln 
        755                 760                 765             


Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln 
    770                 775                 780                 


Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala 
785                 790                 795                 800 


Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala 
                805                 810                 815     


Gly His Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr 
            820                 825                 830         


Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser 
        835                 840                 845             


Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala 
    850                 855                 860                 


Leu Gly Ile Gly Leu Phe Met 
865                 870     


<210>  37
<211>  3162
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD33 EQ CAR preceded by a GMCSR signal sequence and followed by a
       T2A skip sequence and EGFRt

<400>  37
atgctgctcc ttgtcacatc cctgctgctg tgcgaactgc cacatcccgc cttcctgctg       60

atcccccaag tgcagctcgt gcagtccgga gccgaagtca agaagcctgg cagctccgtc      120

aaggtgtcct gcaaagcctc cggctacacc tttaccgact acaacatgca ctgggtccgc      180

caagcacctg gacagggact ggagtggatt gggtacatct acccttacaa cggaggcacc      240

gggtacaacc agaagttcaa gtcgaaggcc accattaccg cggacgaatc caccaacacc      300

gcgtatatgg agctctcatc cttgcggtcg gaggacactg ccgtgtacta ctgcgcgagg      360

ggtagaccgg caatggacta ctggggccag ggcactctcg tcaccgtgtc ctctggtggt      420

ggaggctcag gaggaggggg atccggtgga ggagggagcg atatccagat gacgcagtca      480

ccctcgtccc tgagcgcttc cgtgggcgat cgcgtgacta tcacttgccg ggcttccgag      540

tccgtggata actacggaat ttcctttatg aactggttcc agcaaaagcc gggaaaggcc      600

ccaaagctcc tgatctacgc cgccagcaat cagggatcgg gagtgccctc acggttctcc      660

gggagcggtt caggcaccga cttcaccctt actatttcga gcctgcaacc tgacgatttc      720

gccacttatt actgccaaca gtccaaggaa gtgccgtgga cgttcggcca ggggaccaag      780

gtggaaatca aggagagcaa atacggaccg ccgtgtccac cctgtcctgc acccgagttc      840

gaaggcggcc cttccgtgtt cctgttcccg ccgaagccca aggacaccct gatgatctcg      900

agaaccccgg aggtgacctg cgtggtggtg gacgtgtccc aggaagatcc cgaggtccag      960

ttcaattggt acgtggacgg cgtggaagtg cacaatgcca agaccaagcc cagagaggaa     1020

cagttccaaa gcacctaccg ggtggtgtcc gtgctgaccg tgctgcacca ggactggctg     1080

aacggcaaag agtacaagtg caaggtgtcc aacaagggcc tgcccagcag catcgagaaa     1140

accatcagca aggccaaggg ccagccccgc gagccccagg tgtacacact gccccccagc     1200

caggaagaga tgaccaagaa ccaggtgtcc ctgacctgcc tggtcaaggg cttctacccc     1260

agcgatatcg ccgtggaatg ggagagcaac ggccagcccg agaacaacta caagaccacc     1320

ccccctgtgc tggacagcga cggcagcttc ttcctgtact cccggctgac cgtggacaag     1380

agccggtggc aggaaggcaa cgtcttcagc tgcagcgtga tgcacgaggc cctgcacaac     1440

cactacaccc agaagtctct gagcctgagc ctgggcaaga tggccctgat cgtgctgggc     1500

ggagtggccg gactgctgct gtttatcggc ctgggcatct tcttcaagcg gggcagaaag     1560

aagctgctgt acatcttcaa gcagcccttc atgcggcccg tgcagaccac ccaggaagag     1620

gacggctgca gctgccggtt ccccgaggaa gaggaaggcg gctgcgagct gggaggcggc     1680

agagtgaagt tcagccggtc cgccgacgcc cctgcctacc agcagggcca gaaccagctg     1740

tacaacgagc tgaacctggg caggcgggag gaatacgacg tgctggacaa gcggagaggc     1800

cgggaccctg agatgggcgg caagcccagg cggaagaacc ctcaggaagg cctgtataac     1860

gaactgcaga aagacaagat ggccgaggcc tacagcgaga tcggcatgaa gggcgagcgg     1920

cggaggggca agggccacga cggcctgtac cagggcctga gcaccgccac caaggatacc     1980

tacgacgccc tgcacatgca ggccctgccc ccaaggctcg agggcggcgg agagggcaga     2040

ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggat gcttctcctg     2100

gtgacaagcc ttctgctctg tgagttacca cacccagcat tcctcctgat cccacgcaaa     2160

gtgtgtaacg gaataggtat tggtgaattt aaagactcac tctccataaa tgctacgaat     2220

attaaacact tcaaaaactg cacctccatc agtggcgatc tccacatcct gccggtggca     2280

tttaggggtg actccttcac acatactcct cctctggatc cacaggaact ggatattctg     2340

aaaaccgtaa aggaaatcac agggtttttg ctgattcagg cttggcctga aaacaggacg     2400

gacctccatg cctttgagaa cctagaaatc atacgcggca ggaccaagca acatggtcag     2460

ttttctcttg cagtcgtcag cctgaacata acatccttgg gattacgctc cctcaaggag     2520

ataagtgatg gagatgtgat aatttcagga aacaaaaatt tgtgctatgc aaatacaata     2580

aactggaaaa aactgtttgg gacctccggt cagaaaacca aaattataag caacagaggt     2640

gaaaacagct gcaaggccac aggccaggtc tgccatgcct tgtgctcccc cgagggctgc     2700

tggggcccgg agcccaggga ctgcgtctct tgccggaatg tcagccgagg cagggaatgc     2760

gtggacaagt gcaaccttct ggagggtgag ccaagggagt ttgtggagaa ctctgagtgc     2820

atacagtgcc acccagagtg cctgcctcag gccatgaaca tcacctgcac aggacgggga     2880

ccagacaact gtatccagtg tgcccactac attgacggcc cccactgcgt caagacctgc     2940

ccggcaggag tcatgggaga aaacaacacc ctggtctgga agtacgcaga cgccggccat     3000

gtgtgccacc tgtgccatcc aaactgcacc tacggatgca ctgggccagg tcttgaaggc     3060

tgtccaacga atgggcctaa gatcccgtcc atcgccactg ggatggtggg ggccctcctc     3120

ttgctgctgg tggtggccct ggggatcggc ctcttcatgt ga                        3162


<210>  38
<211>  1053
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD33 EQ CAR preceded by a GMCSR signal sequence and followed by a
       T2A skip sequence and EGFRt

<400>  38

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu 
            20                  25                  30          


Val Lys Lys Pro Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Asp Tyr Asn Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Gln Gly Leu Glu Trp Ile Gly Tyr Ile Tyr Pro Tyr Asn Gly Gly Thr 
65                  70                  75                  80  


Gly Tyr Asn Gln Lys Phe Lys Ser Lys Ala Thr Ile Thr Ala Asp Glu 
                85                  90                  95      


Ser Thr Asn Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Gly Arg Pro Ala Met Asp Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser 
145                 150                 155                 160 


Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys 
                165                 170                 175     


Arg Ala Ser Glu Ser Val Asp Asn Tyr Gly Ile Ser Phe Met Asn Trp 
            180                 185                 190         


Phe Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala 
        195                 200                 205             


Ser Asn Gln Gly Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser 
    210                 215                 220                 


Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Asp Asp Phe 
225                 230                 235                 240 


Ala Thr Tyr Tyr Cys Gln Gln Ser Lys Glu Val Pro Trp Thr Phe Gly 
                245                 250                 255     


Gln Gly Thr Lys Val Glu Ile Lys Glu Ser Lys Tyr Gly Pro Pro Cys 
            260                 265                 270         


Pro Pro Cys Pro Ala Pro Glu Phe Glu Gly Gly Pro Ser Val Phe Leu 
        275                 280                 285             


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
    290                 295                 300                 


Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
305                 310                 315                 320 


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
                325                 330                 335     


Pro Arg Glu Glu Gln Phe Gln Ser Thr Tyr Arg Val Val Ser Val Leu 
            340                 345                 350         


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
        355                 360                 365             


Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
    370                 375                 380                 


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
385                 390                 395                 400 


Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
                405                 410                 415     


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
            420                 425                 430         


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
        435                 440                 445             


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
    450                 455                 460                 


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
465                 470                 475                 480 


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys Met Ala Leu 
                485                 490                 495     


Ile Val Leu Gly Gly Val Ala Gly Leu Leu Leu Phe Ile Gly Leu Gly 
            500                 505                 510         


Ile Phe Phe Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
        515                 520                 525             


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
    530                 535                 540                 


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Gly Gly Gly 
545                 550                 555                 560 


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
                565                 570                 575     


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            580                 585                 590         


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        595                 600                 605             


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    610                 615                 620                 


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
625                 630                 635                 640 


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                645                 650                 655     


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            660                 665                 670         


Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp 
        675                 680                 685             


Val Glu Glu Asn Pro Gly Pro Arg Met Leu Leu Leu Val Thr Ser Leu 
    690                 695                 700                 


Leu Leu Cys Glu Leu Pro His Pro Ala Phe Leu Leu Ile Pro Arg Lys 
705                 710                 715                 720 


Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile 
                725                 730                 735     


Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly 
            740                 745                 750         


Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His 
        755                 760                 765             


Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys 
    770                 775                 780                 


Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr 
785                 790                 795                 800 


Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys 
                805                 810                 815     


Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser 
            820                 825                 830         


Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile 
        835                 840                 845             


Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys 
    850                 855                 860                 


Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly 
865                 870                 875                 880 


Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser 
                885                 890                 895     


Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg 
            900                 905                 910         


Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu 
        915                 920                 925             


Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His 
    930                 935                 940                 


Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly 
945                 950                 955                 960 


Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys 
                965                 970                 975     


Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val 
            980                 985                 990         


Trp Lys Tyr Ala Asp Ala Gly His  Val Cys His Leu Cys  His Pro Asn 
        995                 1000                 1005             


Cys Thr  Tyr Gly Cys Thr Gly  Pro Gly Leu Glu Gly  Cys Pro Thr 
    1010                 1015                 1020             


Asn Gly  Pro Lys Ile Pro Ser  Ile Ala Thr Gly Met  Val Gly Ala 
    1025                 1030                 1035             


Leu Leu  Leu Leu Leu Val Val  Ala Leu Gly Ile Gly  Leu Phe Met 
    1040                 1045                 1050             


