                         SEQUENCE LISTING

<110>  APSE, INC.
       Killmer, John L.
       Kumar, Anil

<120>  METHODS AND COMPOSITIONS FOR MICROBIAL DELIVERY OF DOUBLE-STRANDED RNA

<130>  103827-5009 WO 01

<140>  PCT/US2020/035339
<141>  2020-05-29

<150>  US 62/854,831
<151>  2019-05-30

<160>  46    

<170>  PatentIn version 3.5

<210>  1
<211>  5498
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10180; ErkA stem loop + capsid protein


<220>
<221>  promoter
<222>  (24)..(42)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (127)..(145)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (175)..(182)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (179)..(384)
<223>  ErkA sense strand gene fragment

<220>
<221>  misc_feature
<222>  (436)..(454)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (504)..(511)
<223>  restriction endonuclease NotI recognition site

<220>
<221>  misc_feature
<222>  (505)..(696)
<223>  ErkA antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (692)..(699)
<223>  restriction endonuclease PacI recognition site

<220>
<221>  misc_feature
<222>  (709)..(727)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (743)..(790)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1116)..(1134)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1207)..(1596)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (1448)..(1495)
<223>  bacteriophage T7 transcription terminator

<400>  1
ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac aacggtttcc       60

ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca tacgccggcc      120

attcaaacat gaggattacc catgtaacct aaggccggtg tccaggcgcg ctccgcgatc      180

gcacgcggac aaagttcctc aatctaatgc tgaagttata aggggacaaa tatttgaagt      240

tggtcctagg tatattaaac tcgcctatat aggtgaagga gcttatggca tggttgtgtc      300

tgcggatgac acgctaacaa accaaagagt tgcaataaaa aaaatatcgc cctttgaaca      360

ccaaacttat tgctactaca gtttaaacgc aatcgcagca aactccggca tctactaata      420

gacgccggcc attcaacatg aggattaccc atgtaaccta agaagacaac aaagaagttc      480

aactctttat gtattgatct tccgcggccg ccaataagtt tggtgttcaa agggcgatat      540

tttttttatt gcaactcttt ggtttgttag cgtgtcatcc gcagacacaa ccatgccata      600

agctccttca cctatatagg cgagtttaat atacctagga ccaacttcaa atatttgtcc      660

ccttataact tcagcattag attgaggaac tttaattaag gagttcaaac atgaggatca      720

cccatgtcga agctcccaca ccctagcata accccttggg gcctctaaac gggtcttgag      780

gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca      840

tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcgggcat      900

gcatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt      960

tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc     1020

cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac     1080

ccgtcctgtg gatccagatc tcgatcccgc gaaattaata cgactcacta tagggagacc     1140

acaacggttt ccctctagat cacaagtttg tacaaaaaag caggctaaga aggagatata     1200

catatggcgt ctaactttac ccaattcgtt ctggttgata acggcggtac gggtgacgtt     1260

accgtagctc cgtccaactt cgccaacggt gttgcggaat ggattagctc taacagccgc     1320

tctcaggcct acaaagtcac gtgctccgtt cgtcagtcta gcgcgcagaa tcgcaaatac     1380

accatcaaag ttgaagtacc gaaagtcgca acgcagaccg taggcggcgt agaactccca     1440

gttgcggcct ggcgctctta cctcaacatg gaactgacta ttccgatttt tgcgacgaac     1500

tccgactgcg aactgattgt taaggcaatg cagggcctgc tgaaagacgg taatccgatc     1560

ccatctgcaa tcgctgctaa ctctggcatt tactaataag cggacgcgct gccaccgctg     1620

agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga     1680

aaggaggaac tatatccggc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa     1740

cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaccgatgcc     1800

cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc     1860

cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg     1920

ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc     1980

ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg     2040

tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt     2100

gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg     2160

cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca     2220

gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca ttggaccgct     2280

gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt     2340

aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc     2400

cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac tccaagaatt     2460

ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg gcagaacata     2520

tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag cgttgggtcc     2580

tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt     2640

tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc     2700

tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa     2760

agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga     2820

tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc     2880

tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt     2940

tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc     3000

atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa     3060

caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg     3120

gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac     3180

cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc     3240

tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga     3300

caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag     3360

tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac     3420

tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca     3480

tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc     3540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg     3600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt     3660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa     3720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct     3780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc     3840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg     3900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct     3960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag     4020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga     4080

agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga     4140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg     4200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag     4260

aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag     4320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat     4380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct     4440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac     4500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa     4560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg     4620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt     4680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca     4740

ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt     4800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct     4860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg     4920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg     4980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg     5040

cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa     5100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt     5160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt     5220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt     5280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca     5340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat     5400

ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata     5460

aaaataggcg tatcacgagg ccctttcgtc ttcaagaa                             5498


<210>  2
<211>  4696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10181; ErkA stem loop - coat protein


<220>
<221>  promoter
<222>  (24)..(42)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (127)..(145)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (175)..(182)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (179)..(384)
<223>  ErkA sense strand gene fragment

<220>
<221>  misc_feature
<222>  (436)..(454)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (504)..(511)
<223>  restriction endonuclease NotI recognition site

<220>
<221>  misc_feature
<222>  (505)..(696)
<223>  ErkA antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (692)..(699)
<223>  restriction endonuclease PacI recognition site

<220>
<221>  misc_feature
<222>  (709)..(727)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (743)..(790)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  misc_feature
<222>  (897)..(902)
<223>  restriction endonuclease SphI recognition site

<400>  2
ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac aacggtttcc       60

ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca tacgccggcc      120

attcaaacat gaggattacc catgtaacct aaggccggtg tccaggcgcg ctccgcgatc      180

gcacgcggac aaagttcctc aatctaatgc tgaagttata aggggacaaa tatttgaagt      240

tggtcctagg tatattaaac tcgcctatat aggtgaagga gcttatggca tggttgtgtc      300

tgcggatgac acgctaacaa accaaagagt tgcaataaaa aaaatatcgc cctttgaaca      360

ccaaacttat tgctactaca gtttaaacgc aatcgcagca aactccggca tctactaata      420

gacgccggcc attcaacatg aggattaccc atgtaaccta agaagacaac aaagaagttc      480

aactctttat gtattgatct tccgcggccg ccaataagtt tggtgttcaa agggcgatat      540

tttttttatt gcaactcttt ggtttgttag cgtgtcatcc gcagacacaa ccatgccata      600

agctccttca cctatatagg cgagtttaat atacctagga ccaacttcaa atatttgtcc      660

ccttataact tcagcattag attgaggaac tttaattaag gagttcaaac atgaggatca      720

cccatgtcga agctcccaca ccctagcata accccttggg gcctctaaac gggtcttgag      780

gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca      840

tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcgggcat      900

gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta      960

atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc     1020

agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt     1080

atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc     1140

tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc     1200

ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt     1260

atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc     1320

tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg     1380

caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc     1440

gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc     1500

gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc     1560

tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc     1620

ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga     1680

gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca     1740

gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg     1800

tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg     1860

aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag     1920

caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag     1980

cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa     2040

cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc     2100

gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat     2160

catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga     2220

acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca     2280

tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg     2340

cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca     2400

gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga     2460

cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag     2520

cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt     2580

atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg     2640

tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc     2700

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa     2760

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa     2820

aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct     2880

ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac     2940

aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc     3000

gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc     3060

tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg     3120

tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga     3180

gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag     3240

cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta     3300

cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag     3360

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg     3420

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac     3480

ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc     3540

aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag     3600

tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc     3660

agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac     3720

gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc     3780

accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg     3840

tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag     3900

tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc     3960

acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac     4020

atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag     4080

aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac     4140

tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg     4200

agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc     4260

gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact     4320

ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg     4380

atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa     4440

tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt     4500

tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg     4560

tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga     4620

cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc     4680

ctttcgtctt caagaa                                                     4696


<210>  3
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide probe

<400>  3
ggccggcgtc tattagtaga tgcc                                              24


<210>  4
<211>  5591
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10189; beta actin sense strand + coat 
       protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (373)..(667)
<223>  beta actin sense strand gene fragment

<220>
<221>  misc_feature
<222>  (667)..(674)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (802)..(820)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (836)..(883)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1209)..(1227)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1300)..(1689)
<223>  bacteriophage MS2 cot protein gene

<220>
<221>  gene
<222>  (1300)..(1689)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (1722)..(1769)
<223>  bacteriophage T7 transcription terminator

<400>  4
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta tgtgcaaagc      480

cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg gtcgcccaag      540

gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag atgaagccca      600

aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca tcaccaactg      660

ggatgagttt aaacctttcg gattataaca tcacatctag gcgcgcctga cgatcaacca      720

taccagacgg accgaatacc cggtctgaac gagggcggcc gcggtaccca agaagtactt      780

agagttaatt aaggagttca aacatgagga tcacccatgt cgaagctccc acaccctagc      840

ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat      900

atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa      960

gtagcgaagc gagcaggact gggcggcggg catgcatcgt ccattccgac agcatcgcca     1020

gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc     1080

tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag     1140

ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggatccag atctcgatcc     1200

cgcgaaatta atacgactca ctatagggag accacaacgg tttccctcta gatcacaagt     1260

ttgtacaaaa aagcaggcta agaaggagat atacatatgg cgtctaactt tacccaattc     1320

gttctggttg ataacggcgg tacgggtgac gttaccgtag ctccgtccaa cttcgccaac     1380

ggtgttgcgg aatggattag ctctaacagc cgctctcagg cctacaaagt cacgtgctcc     1440

gttcgtcagt ctagcgcgca gaatcgcaaa tacaccatca aagttgaagt accgaaagtc     1500

gcaacgcaga ccgtaggcgg cgtagaactc ccagttgcgg cctggcgctc ttacctcaac     1560

atggaactga ctattccgat ttttgcgacg aactccgact gcgaactgat tgttaaggca     1620

atgcagggcc tgctgaaaga cggtaatccg atcccatctg caatcgctgc taactctggc     1680

atttactaat aagcggacgc gctgccaccg ctgagcaata actagcataa ccccttgggg     1740

cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggcatgcacc     1800

attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca     1860

ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc     1920

cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat     1980

gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg     2040

ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc     2100

tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc     2160

cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat     2220

ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc     2280

catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc     2340

tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt atgccgcctc     2400

ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct     2460

ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg     2520

cacctcgcta acggattcac cactccaaga attggagcca atcaattctt gcggagaact     2580

gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat ctccagcagc     2640

cgcacgcggc gcatctcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc     2700

ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca     2760

ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca     2820

acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc     2880

tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct     2940

acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc     3000

atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca     3060

gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga     3120

aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc     3180

cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat     3240

gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc     3300

ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc     3360

acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt     3420

gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact     3480

ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa     3540

taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca     3600

ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg     3660

taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc     3720

agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc     3780

cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac     3840

tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc     3900

tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata     3960

gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc     4020

acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca     4080

acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag     4140

cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta     4200

gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg     4260

gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc     4320

agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt     4380

ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa     4440

ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat     4500

atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga     4560

tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac     4620

gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg     4680

ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg     4740

caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt     4800

cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct     4860

cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat     4920

cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta     4980

agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca     5040

tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat     5100

agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac     5160

atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa     5220

ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt     5280

cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg     5340

caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat     5400

attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt     5460

agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct     5520

aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc     5580

gtcttcaaga a                                                          5591


<210>  5
<211>  5591
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10190; beta actin antisense strand + 
       coat protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (373)..(667)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (667)..(674)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (802)..(820)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (836)..(883)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1209)..(1227)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1300)..(1689)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (1722)..(1769)
<223>  bacteriophage T7 transcription terminator

<400>  5
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gctcatccca gttggtgatg ataccgtgtt cgatggggta tttcagggtg      420

aggatacctc ttttgctttg ggcttcatct cctacgtatg agtccttttg tcccataccg      480

accatgactc cttgatgcct tgggcgaccg acgatcgagg ggaagacggc acggggtgcg      540

tcatctcctg cgaaaccggc tttgcacata ccggatccat tgtctacgac aagagccgct      600

acatcgtcgt cacacatgtt gtcttttgag gttggacact gctcactaga cagaaaaacc      660

tcgtgcgttt aaacctttcg gattataaca tcacatctag gcgcgcctga cgatcaacca      720

taccagacgg accgaatacc cggtctgaac gagggcggcc gcggtaccca agaagtactt      780

agagttaatt aaggagttca aacatgagga tcacccatgt cgaagctccc acaccctagc      840

ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat      900

atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa      960

gtagcgaagc gagcaggact gggcggcggg catgcatcgt ccattccgac agcatcgcca     1020

gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc     1080

tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag     1140

ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggatccag atctcgatcc     1200

cgcgaaatta atacgactca ctatagggag accacaacgg tttccctcta gatcacaagt     1260

ttgtacaaaa aagcaggcta agaaggagat atacatatgg cgtctaactt tacccaattc     1320

gttctggttg ataacggcgg tacgggtgac gttaccgtag ctccgtccaa cttcgccaac     1380

ggtgttgcgg aatggattag ctctaacagc cgctctcagg cctacaaagt cacgtgctcc     1440

gttcgtcagt ctagcgcgca gaatcgcaaa tacaccatca aagttgaagt accgaaagtc     1500

gcaacgcaga ccgtaggcgg cgtagaactc ccagttgcgg cctggcgctc ttacctcaac     1560

atggaactga ctattccgat ttttgcgacg aactccgact gcgaactgat tgttaaggca     1620

atgcagggcc tgctgaaaga cggtaatccg atcccatctg caatcgctgc taactctggc     1680

atttactaat aagcggacgc gctgccaccg ctgagcaata actagcataa ccccttgggg     1740

cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggcatgcacc     1800

attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca     1860

ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc     1920

cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat     1980

gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg     2040

ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc     2100

tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc     2160

cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat     2220

ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc     2280

catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc     2340

tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt atgccgcctc     2400

ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct     2460

ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg     2520

cacctcgcta acggattcac cactccaaga attggagcca atcaattctt gcggagaact     2580

gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat ctccagcagc     2640

cgcacgcggc gcatctcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc     2700

ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca     2760

ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca     2820

acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc     2880

tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct     2940

acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc     3000

atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca     3060

gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga     3120

aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc     3180

cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat     3240

gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc     3300

ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc     3360

acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt     3420

gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact     3480

ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa     3540

taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca     3600

ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg     3660

taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc     3720

agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc     3780

cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac     3840

tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc     3900

tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata     3960

gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc     4020

acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca     4080

acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag     4140

cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta     4200

gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg     4260

gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc     4320

agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt     4380

ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa     4440

ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat     4500

atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga     4560

tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac     4620

gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg     4680

ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg     4740

caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt     4800

cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct     4860

cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat     4920

cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta     4980

agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca     5040

tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat     5100

agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac     5160

atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa     5220

ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt     5280

cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg     5340

caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat     5400

attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt     5460

agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct     5520

aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc     5580

gtcttcaaga a                                                          5591


<210>  6
<211>  4789
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10274; beta actin sense strand - coat 
       protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (373)..(667)
<223>  beta actin sense strand gene fragment

<220>
<221>  misc_feature
<222>  (667)..(674)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (802)..(820)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (836)..(883)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  misc_feature
<222>  (990)..(995)
<223>  restriction endonuclease SphI  recognition site

<400>  6
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta tgtgcaaagc      480

cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg gtcgcccaag      540

gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag atgaagccca      600

aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca tcaccaactg      660

ggatgagttt aaacctttcg gattataaca tcacatctag gcgcgcctga cgatcaacca      720

taccagacgg accgaatacc cggtctgaac gagggcggcc gcggtaccca agaagtactt      780

agagttaatt aaggagttca aacatgagga tcacccatgt cgaagctccc acaccctagc      840

ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat      900

atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa      960

gtagcgaagc gagcaggact gggcggcggg catgcaccat tccttgcggc ggcggtgctc     1020

aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt     1080

cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg     1140

actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg     1200

gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc     1260

ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc     1320

gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg     1380

ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt     1440

ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat     1500

gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc     1560

attggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg     1620

gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca     1680

tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca     1740

ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt     1800

ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca     1860

gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag     1920

gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa     1980

gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc     2040

gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct     2100

gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct     2160

ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac     2220

cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc     2280

tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat     2340

cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat     2400

taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat     2460

cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg     2520

gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg     2580

ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag     2640

ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga     2700

gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag     2760

aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt     2820

tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc     2880

aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa     2940

aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa     3000

tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc     3060

ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc     3120

cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag     3180

ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga     3240

ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc     3300

gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac     3360

agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg     3420

cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca     3480

aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa     3540

aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa     3600

ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt     3660

aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag     3720

ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat     3780

agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc     3840

cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa     3900

ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca     3960

gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa     4020

cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt     4080

cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc     4140

ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact     4200

catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc     4260

tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg     4320

ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct     4380

catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc     4440

cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag     4500

cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac     4560

acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg     4620

ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt     4680

tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac     4740

attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaa                 4789


<210>  7
<211>  4789
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10275; beta actin antisense strand - 
       coat protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (373)..(667)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (667)..(674)
<223>  restriction endonuclease PmeI recogntion site

<220>
<221>  misc_feature
<222>  (802)..(820)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (836)..(883)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  misc_feature
<222>  (990)..(995)
<223>  restriction endonuclease SphI recognition site

<400>  7
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gctcatccca gttggtgatg ataccgtgtt cgatggggta tttcagggtg      420

aggatacctc ttttgctttg ggcttcatct cctacgtatg agtccttttg tcccataccg      480

accatgactc cttgatgcct tgggcgaccg acgatcgagg ggaagacggc acggggtgcg      540

tcatctcctg cgaaaccggc tttgcacata ccggatccat tgtctacgac aagagccgct      600

acatcgtcgt cacacatgtt gtcttttgag gttggacact gctcactaga cagaaaaacc      660

tcgtgcgttt aaacctttcg gattataaca tcacatctag gcgcgcctga cgatcaacca      720

taccagacgg accgaatacc cggtctgaac gagggcggcc gcggtaccca agaagtactt      780

agagttaatt aaggagttca aacatgagga tcacccatgt cgaagctccc acaccctagc      840

ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat      900

atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa      960

gtagcgaagc gagcaggact gggcggcggg catgcaccat tccttgcggc ggcggtgctc     1020

aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt     1080

cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg     1140

actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg     1200

gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc     1260

ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc     1320

gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg     1380

ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt     1440

ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat     1500

gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc     1560

attggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg     1620

gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca     1680

tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca     1740

ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt     1800

ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca     1860

gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag     1920

gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa     1980

gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc     2040

gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct     2100

gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct     2160

ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac     2220

cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc     2280

tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat     2340

cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat     2400

taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat     2460

cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg     2520

gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg     2580

ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag     2640

ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga     2700

gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag     2760

aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt     2820

tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc     2880

aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa     2940

aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa     3000

tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc     3060

ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc     3120

cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag     3180

ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga     3240

ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc     3300

gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac     3360

agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg     3420

cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca     3480

aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa     3540

aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa     3600

ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt     3660

aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag     3720

ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat     3780

agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc     3840

cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa     3900

ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca     3960

gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa     4020

cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt     4080

cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc     4140

ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact     4200

catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc     4260

tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg     4320

ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct     4380

catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc     4440

cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag     4500

cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac     4560

acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg     4620

ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt     4680

tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac     4740

attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaa                 4789


<210>  8
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10269; beta actin stem loop + coat 
       protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (373)..(667)
<223>  beta actin sense strand gene fragment

<220>
<221>  misc_feature
<222>  (667)..(674)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (735)..(753)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (832)..(1126)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (1201)..(1219)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (1235)..(1282)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1608)..(1626)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1699)..(2088)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (2121)..(2168)
<223>  bacteriophage T7 transcription terminator

<400>  8
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta tgtgcaaagc      480

cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg gtcgcccaag      540

gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag atgaagccca      600

aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca tcaccaactg      660

ggatgagttt aaaccctcta gctgctttac aaagtactgg ttccctttcc agcgggatgc      720

tttatctaaa cgcaacatga ggatcaccca tgtcgccaca tcgcttccta gttccgctgg      780

gatccatcgt tggcggccga agccgccatt ccatagtgag ttctggcgcg cctcatccca      840

gttggtgatg ataccgtgtt cgatggggta tttcagggtg aggatacctc ttttgctttg      900

ggcttcatct cctacgtatg agtccttttg tcccataccg accatgactc cttgatgcct      960

tgggcgaccg acgatcgagg ggaagacggc acggggtgcg tcatctcctg cgaaaccggc     1020

tttgcacata ccggatccat tgtctacgac aagagccgct acatcgtcgt cacacatgtt     1080

gtcttttgag gttggacact gctcactaga cagaaaaacc tcgtgccgga ccgaataccc     1140

ggtctgaacg agggcggccg cggtacccaa gaagtactta gagttaatta aggagttcaa     1200

acatgaggat cacccatgtc gaagctccca caccctagca taaccccttg gggcctctaa     1260

acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg     1320

gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg     1380

ggcggcgggc atgcatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag     1440

cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct     1500

ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca     1560

tggcgaccac acccgtcctg tggatccaga tctcgatccc gcgaaattaa tacgactcac     1620

tatagggaga ccacaacggt ttccctctag atcacaagtt tgtacaaaaa agcaggctaa     1680

gaaggagata tacatatggc gtctaacttt acccaattcg ttctggttga taacggcggt     1740

acgggtgacg ttaccgtagc tccgtccaac ttcgccaacg gtgttgcgga atggattagc     1800

tctaacagcc gctctcaggc ctacaaagtc acgtgctccg ttcgtcagtc tagcgcgcag     1860

aatcgcaaat acaccatcaa agttgaagta ccgaaagtcg caacgcagac cgtaggcggc     1920

gtagaactcc cagttgcggc ctggcgctct tacctcaaca tggaactgac tattccgatt     1980

tttgcgacga actccgactg cgaactgatt gttaaggcaa tgcagggcct gctgaaagac     2040

ggtaatccga tcccatctgc aatcgctgct aactctggca tttactaata agcggacgcg     2100

ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg     2160

gttttttgct gaaaggagga actatatccg gcatgcacca ttccttgcgg cggcggtgct     2220

caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg     2280

tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat     2340

gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc     2400

ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg     2460

cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc     2520

cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct     2580

gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct     2640

tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga     2700

tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat     2760

cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt     2820

ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc     2880

atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc     2940

actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct     3000

tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc     3060

agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta     3120

ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga     3180

agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc     3240

cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc     3300

tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc     3360

tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta     3420

ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc     3480

ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca     3540

tcagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca     3600

ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa     3660

tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac     3720

ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat     3780

gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca     3840

gccatgaccc agtcacgtag cgat                                            3864


<210>  9
<211>  5375
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10306; beta actin stem loop - coat 
       protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (373)..(667)
<223>  beta actin sense strand gene fragment

<220>
<221>  misc_feature
<222>  (667)..(674)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (735)..(753)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (832)..(1126)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (1201)..(1219)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (1235)..(1282)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  misc_feature
<222>  (1389)..(1394)
<223>  restriction endonuclease SphI recognition site

<400>  9
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta tgtgcaaagc      480

cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg gtcgcccaag      540

gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag atgaagccca      600

aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca tcaccaactg      660

ggatgagttt aaaccctcta gctgctttac aaagtactgg ttccctttcc agcgggatgc      720

tttatctaaa cgcaacatga ggatcaccca tgtcgccaca tcgcttccta gttccgctgg      780

gatccatcgt tggcggccga agccgccatt ccatagtgag ttctggcgcg cctcatccca      840

gttggtgatg ataccgtgtt cgatggggta tttcagggtg aggatacctc ttttgctttg      900

ggcttcatct cctacgtatg agtccttttg tcccataccg accatgactc cttgatgcct      960

tgggcgaccg acgatcgagg ggaagacggc acggggtgcg tcatctcctg cgaaaccggc     1020

tttgcacata ccggatccat tgtctacgac aagagccgct acatcgtcgt cacacatgtt     1080

gtcttttgag gttggacact gctcactaga cagaaaaacc tcgtgccgga ccgaataccc     1140

ggtctgaacg agggcggccg cggtacccaa gaagtactta gagttaatta aggagttcaa     1200

acatgaggat cacccatgtc gaagctccca caccctagca taaccccttg gggcctctaa     1260

acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg     1320

gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg     1380

ggcggcgggc atgcatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag     1440

cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct     1500

ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca     1560

tggcgaccac acccgtcctg taccattcct tgcggcggcg gtgctcaacg gcctcaacct     1620

actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgac cgatgccctt     1680

gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc     1740

acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt     1800

cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt     1860

attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt     1920

cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct     1980

ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg     2040

catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg     2100

acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcattg gaccgctgat     2160

cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg     2220

cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac     2280

ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga     2340

gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc     2400

atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg     2460

ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc     2520

cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc     2580

aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt     2640

ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc     2700

tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga     2760

gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc     2820

agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc     2880

ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag     2940

gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag     3000

aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac     3060

gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga     3120

cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa     3180

gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca     3240

cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga     3300

gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca     3360

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag     3420

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag     3480

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc     3540

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc     3600

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc     3660

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt     3720

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg     3780

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat     3840

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     3900

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     3960

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     4020

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     4080

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     4140

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     4200

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     4260

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     4320

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     4380

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     4440

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     4500

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     4560

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     4620

ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     4680

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     4740

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     4800

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     4860

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     4920

caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     4980

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     5040

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     5100

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     5160

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     5220

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     5280

cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa     5340

ataggcgtat cacgaggccc tttcgtcttc aagaa                                5375


<210>  10
<211>  5990
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10216; beta actin stem loop + coat 
       protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (373)..(667)
<223>  beta actin sense strand gene fragment

<220>
<221>  misc_feature
<222>  (667)..(674)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (832)..(1126)
<223>  beta actin antisense gene fragment

<220>
<221>  misc_feature
<222>  (1201)..(1219)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (1235)..(1282)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1608)..(1626)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1699)..(2088)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (2121)..(2168)
<223>  bacteriophage T7 transcription terminator

<400>  10
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta tgtgcaaagc      480

cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg gtcgcccaag      540

gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag atgaagccca      600

aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca tcaccaactg      660

ggatgagttt aaaccctcta gctgctttac aaagtactgg ttccctttcc agcgggatgc      720

tttatctaaa cgcaatgaga gaggtattcc tcaggccaca tcgcttccta gttccgctgg      780

gatccatcgt tggcggccga agccgccatt ccatagtgag ttctggcgcg cctcatccca      840

gttggtgatg ataccgtgtt cgatggggta tttcagggtg aggatacctc ttttgctttg      900

ggcttcatct cctacgtatg agtccttttg tcccataccg accatgactc cttgatgcct      960

tgggcgaccg acgatcgagg ggaagacggc acggggtgcg tcatctcctg cgaaaccggc     1020

tttgcacata ccggatccat tgtctacgac aagagccgct acatcgtcgt cacacatgtt     1080

gtcttttgag gttggacact gctcactaga cagaaaaacc tcgtgccgga ccgaataccc     1140

ggtctgaacg agggcggccg cggtacccaa gaagtactta gagttaatta aggagttcaa     1200

acatgaggat cacccatgtc gaagctccca caccctagca taaccccttg gggcctctaa     1260

acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg     1320

gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg     1380

ggcggcgggc atgcatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag     1440

cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct     1500

ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca     1560

tggcgaccac acccgtcctg tggatccaga tctcgatccc gcgaaattaa tacgactcac     1620

tatagggaga ccacaacggt ttccctctag atcacaagtt tgtacaaaaa agcaggctaa     1680

gaaggagata tacatatggc gtctaacttt acccaattcg ttctggttga taacggcggt     1740

acgggtgacg ttaccgtagc tccgtccaac ttcgccaacg gtgttgcgga atggattagc     1800

tctaacagcc gctctcaggc ctacaaagtc acgtgctccg ttcgtcagtc tagcgcgcag     1860

aatcgcaaat acaccatcaa agttgaagta ccgaaagtcg caacgcagac cgtaggcggc     1920

gtagaactcc cagttgcggc ctggcgctct tacctcaaca tggaactgac tattccgatt     1980

tttgcgacga actccgactg cgaactgatt gttaaggcaa tgcagggcct gctgaaagac     2040

ggtaatccga tcccatctgc aatcgctgct aactctggca tttactaata agcggacgcg     2100

ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg     2160

gttttttgct gaaaggagga actatatccg gcatgcacca ttccttgcgg cggcggtgct     2220

caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg     2280

tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat     2340

gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc     2400

ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg     2460

cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc     2520

cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct     2580

gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct     2640

tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga     2700

tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat     2760

cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt     2820

ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc     2880

atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc     2940

actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct     3000

tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc     3060

agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta     3120

ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga     3180

agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc     3240

cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc     3300

tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc     3360

tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta     3420

ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc     3480

ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca     3540

tcagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca     3600

ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa     3660

tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac     3720

ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat     3780

gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca     3840

gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag     3900

agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga     3960

gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg     4020

ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat     4080

caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta     4140

aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa     4200

atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc     4260

cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt     4320

ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca     4380

gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg     4440

accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat     4500

cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta     4560

cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct     4620

gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac     4680

aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa     4740

aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa     4800

actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt     4860

taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca     4920

gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca     4980

tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc     5040

ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa     5100

accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc     5160

agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca     5220

acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat     5280

tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag     5340

cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac     5400

tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt     5460

ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt     5520

gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc     5580

tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat     5640

ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca     5700

gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga     5760

cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg     5820

gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg     5880

ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga     5940

cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa                5990


<210>  11
<211>  5188
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10305; beta actin stem loop - coat 
       protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (373)..(667)
<223>  beta actin sense strand gene fragment

<220>
<221>  misc_feature
<222>  (667)..(674)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (832)..(1126)
<223>  beta actin antisense gene fragment

<220>
<221>  misc_feature
<222>  (1201)..(1219)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (1235)..(1282)
<223>  bacteriophageT7 transcription terminator

<220>
<221>  misc_feature
<222>  (1389)..(1394)
<223>  restriction endonuclease SphI recognition site

<400>  11
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta tgtgcaaagc      480

cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg gtcgcccaag      540

gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag atgaagccca      600

aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca tcaccaactg      660

ggatgagttt aaaccctcta gctgctttac aaagtactgg ttccctttcc agcgggatgc      720

tttatctaaa cgcaatgaga gaggtattcc tcaggccaca tcgcttccta gttccgctgg      780

gatccatcgt tggcggccga agccgccatt ccatagtgag ttctggcgcg cctcatccca      840

gttggtgatg ataccgtgtt cgatggggta tttcagggtg aggatacctc ttttgctttg      900

ggcttcatct cctacgtatg agtccttttg tcccataccg accatgactc cttgatgcct      960

tgggcgaccg acgatcgagg ggaagacggc acggggtgcg tcatctcctg cgaaaccggc     1020

tttgcacata ccggatccat tgtctacgac aagagccgct acatcgtcgt cacacatgtt     1080

gtcttttgag gttggacact gctcactaga cagaaaaacc tcgtgccgga ccgaataccc     1140

ggtctgaacg agggcggccg cggtacccaa gaagtactta gagttaatta aggagttcaa     1200

acatgaggat cacccatgtc gaagctccca caccctagca taaccccttg gggcctctaa     1260

acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg     1320

gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg     1380

ggcggcgggc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg     1440

ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc     1500

ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg     1560

actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc     1620

ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga     1680

atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag     1740

aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc     1800

gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg     1860

atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt     1920

caaggatcgc tcgcggctct taccagccta acttcgatca ttggaccgct gatcgtcacg     1980

gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc     2040

ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc     2100

tgaatggaag ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc     2160

aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt     2220

ccgccatctc cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg     2280

tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg     2340

gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt     2400

ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa     2460

cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc     2520

taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt     2580

ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc     2640

gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca     2700

ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa     2760

ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca     2820

acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg     2880

agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc     2940

agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc     3000

agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg     3060

atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca     3120

ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc     3180

ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc     3240

agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa     3300

catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt     3360

tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg     3420

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg     3480

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag     3540

cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc     3600

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa     3660

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg     3720

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc     3780

taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac     3840

cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg     3900

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt     3960

gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt     4020

catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa     4080

atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga     4140

ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt     4200

gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg     4260

agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga     4320

gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga     4380

agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg     4440

catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc     4500

aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc     4560

gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca     4620

taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac     4680

caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg     4740

ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc     4800

ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg     4860

tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac     4920

aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat     4980

actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata     5040

catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa     5100

agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg     5160

tatcacgagg ccctttcgtc ttcaagaa                                        5188


<210>  12
<211>  5657
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10219; beta actin stem loop + coat 
       protein


<220>
<221>  promoter
<222>  (10)..(28)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (32)..(39)
<223>  restriction endonuclease AsiSI recognition site sequence

<220>
<221>  misc_feature
<222>  (40)..(334)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (334)..(341)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (497)..(791)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (868)..(886)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (902)..(949)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1275)..(1293)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1366)..(1755)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (1788)..(1835)
<223>  bacteriophage T7 transcription terminator

<400>  12
ttccgaaatt aatacgactc actataggga ggcgatcgcg cacgaggttt ttctgtctag       60

tgagcagtgt ccaacctcaa aagacaacat gtgtgacgac gatgtagcgg ctcttgtcgt      120

agacaatgga tccggtatgt gcaaagccgg tttcgcagga gatgacgcac cccgtgccgt      180

cttcccctcg atcgtcggtc gcccaaggca tcaaggagtc atggtcggta tgggacaaaa      240

ggactcatac gtaggagatg aagcccaaag caaaagaggt atcctcaccc tgaaataccc      300

catcgaacac ggtatcatca ccaactggga tgagtttaaa ccctctagct gctttacaaa      360

gtactggttc cctttccagc gggatgcttt atctaaacgc aatgagagag gtattcctca      420

ggccacatcg cttcctagtt ccgctgggat ccatcgttgg cggccgaagc cgccattcca      480

tagtgagttc tggcgcgcct catcccagtt ggtgatgata ccgtgttcga tggggtattt      540

cagggtgagg atacctcttt tgctttgggc ttcatctcct acgtatgagt ccttttgtcc      600

cataccgacc atgactcctt gatgccttgg gcgaccgacg atcgagggga agacggcacg      660

gggtgcgtca tctcctgcga aaccggcttt gcacataccg gatccattgt ctacgacaag      720

agccgctaca tcgtcgtcac acatgttgtc ttttgaggtt ggacactgct cactagacag      780

aaaaacctcg tgccggaccg aatacccggt ctgaacgagg gcggccgcgg tacccaagaa      840

gtacttagag ttaattaagg agttcaaaca tgaggatcac ccatgtcgaa gctcccacac      900

cctagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg      960

aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg     1020

ctccaagtag cgaagcgagc aggactgggc ggcgggcatg catcgtccat tccgacagca     1080

tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac     1140

ccgttctcgg agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac     1200

ttggagccac tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg atccagatct     1260

cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagatc     1320

acaagtttgt acaaaaaagc aggctaagaa ggagatatac atatggcgtc taactttacc     1380

caattcgttc tggttgataa cggcggtacg ggtgacgtta ccgtagctcc gtccaacttc     1440

gccaacggtg ttgcggaatg gattagctct aacagccgct ctcaggccta caaagtcacg     1500

tgctccgttc gtcagtctag cgcgcagaat cgcaaataca ccatcaaagt tgaagtaccg     1560

aaagtcgcaa cgcagaccgt aggcggcgta gaactcccag ttgcggcctg gcgctcttac     1620

ctcaacatgg aactgactat tccgattttt gcgacgaact ccgactgcga actgattgtt     1680

aaggcaatgc agggcctgct gaaagacggt aatccgatcc catctgcaat cgctgctaac     1740

tctggcattt actaataagc ggacgcgctg ccaccgctga gcaataacta gcataacccc     1800

ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggca     1860

tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct     1920

aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt     1980

cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt     2040

tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg     2100

ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc     2160

cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat     2220

tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg     2280

ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt     2340

gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct     2400

cgcggctctt accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc     2460

cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt     2520

ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc     2580

cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg     2640

agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc     2700

agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc     2760

gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat     2820

gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga     2880

gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca     2940

gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga     3000

acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc     3060

cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca     3120

tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg     3180

aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac     3240

atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac     3300

gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc     3360

agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag     3420

acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca     3480

gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg     3540

tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt     3600

gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct     3660

cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa     3720

aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa     3780

aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc     3840

tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga     3900

caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc     3960

cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt     4020

ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct     4080

gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg     4140

agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta     4200

gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct     4260

acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa     4320

gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt     4380

gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta     4440

cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat     4500

caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa     4560

gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct     4620

cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta     4680

cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct     4740

caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg     4800

gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa     4860

gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt     4920

cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta     4980

catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca     5040

gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta     5100

ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct     5160

gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg     5220

cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac     5280

tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact     5340

gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa     5400

atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt     5460

ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat     5520

gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg     5580

acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc     5640

cctttcgtct tcaagaa                                                    5657


<210>  13
<211>  4855
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10304; beta actin stem loop - coat 
       protein


<220>
<221>  promoter
<222>  (10)..(28)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (32)..(39)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (40)..(334)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (334)..(341)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (497)..(791)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (868)..(886)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (902)..(949)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  misc_feature
<222>  (1056)..(1061)
<223>  restriction endonuclease SphI recognition site

<400>  13
ttccgaaatt aatacgactc actataggga ggcgatcgcg cacgaggttt ttctgtctag       60

tgagcagtgt ccaacctcaa aagacaacat gtgtgacgac gatgtagcgg ctcttgtcgt      120

agacaatgga tccggtatgt gcaaagccgg tttcgcagga gatgacgcac cccgtgccgt      180

cttcccctcg atcgtcggtc gcccaaggca tcaaggagtc atggtcggta tgggacaaaa      240

ggactcatac gtaggagatg aagcccaaag caaaagaggt atcctcaccc tgaaataccc      300

catcgaacac ggtatcatca ccaactggga tgagtttaaa ccctctagct gctttacaaa      360

gtactggttc cctttccagc gggatgcttt atctaaacgc aatgagagag gtattcctca      420

ggccacatcg cttcctagtt ccgctgggat ccatcgttgg cggccgaagc cgccattcca      480

tagtgagttc tggcgcgcct catcccagtt ggtgatgata ccgtgttcga tggggtattt      540

cagggtgagg atacctcttt tgctttgggc ttcatctcct acgtatgagt ccttttgtcc      600

cataccgacc atgactcctt gatgccttgg gcgaccgacg atcgagggga agacggcacg      660

gggtgcgtca tctcctgcga aaccggcttt gcacataccg gatccattgt ctacgacaag      720

agccgctaca tcgtcgtcac acatgttgtc ttttgaggtt ggacactgct cactagacag      780

aaaaacctcg tgccggaccg aatacccggt ctgaacgagg gcggccgcgg tacccaagaa      840

gtacttagag ttaattaagg agttcaaaca tgaggatcac ccatgtcgaa gctcccacac      900

cctagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg      960

aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg     1020

ctccaagtag cgaagcgagc aggactgggc ggcgggcatg caccattcct tgcggcggcg     1080

gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga     1140

gagcgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg     1200

ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag     1260

gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg     1320

atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact     1380

ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac     1440

gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg     1500

attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag     1560

gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact     1620

tcgatcattg gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac     1680

gggttggcat ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc     1740

ggtgcatgga gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat     1800

tcaccactcc aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca     1860

acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct     1920

cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc     1980

ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa     2040

cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg     2100

gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc     2160

ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga     2220

agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt     2280

gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga     2340

gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg     2400

aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc     2460

agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct     2520

gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg     2580

atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag     2640

cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg     2700

gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc     2760

atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt     2820

aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc     2880

ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac     2940

agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa     3000

ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca     3060

caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc     3120

gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata     3180

cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta     3240

tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca     3300

gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga     3360

cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg     3420

tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg     3480

tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg     3540

caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag     3600

aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa     3660

cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat     3720

ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc     3780

tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc     3840

atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc     3900

tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc     3960

aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc     4020

catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt     4080

gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc     4140

ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa     4200

aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt     4260

atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg     4320

cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc     4380

gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa     4440

agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt     4500

gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt     4560

caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag     4620

ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta     4680

tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat     4740

aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat     4800

catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaa          4855


<210>  14
<211>  5367
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10279; beta actin stem loop + capsid


<220>
<221>  promoter
<222>  (38)..(56)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (60)..(67)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (68)..(362)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (362)..(369)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (527)..(821)
<223>  beta actin antisense strand gene fragment

<220>
<221>  terminator
<222>  (887)..(934)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (985)..(1003)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1076)..(1465)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (1498)..(1545)
<223>  bacteriophage T7 transcription terminator

<400>  14
ttcttagctc cggcaagcaa ttaagaactt ccgaaattaa tacgactcac tatagggagg       60

cgatcgcgca cgaggttttt ctgtctagtg agcagtgtcc aacctcaaaa gacaacatgt      120

gtgacgacga tgtagcggct cttgtcgtag acaatggatc cggtatgtgc aaagccggtt      180

tcgcaggaga tgacgcaccc cgtgccgtct tcccctcgat cgtcggtcgc ccaaggcatc      240

aaggagtcat ggtcggtatg ggacaaaagg actcatacgt aggagatgaa gcccaaagca      300

aaagaggtat cctcaccctg aaatacccca tcgaacacgg tatcatcacc aactgggatg      360

agtttaaacc ctctagctgc tttacaaagt actggttccc tttccagcgg gatgctttat      420

ctaaacgcaa tgagagaggt attcctcagg ccacatcgct tcctagttcc gctgggatcc      480

atcgttggcg gccgaagccg ccattccata gtgagttctg gcgcgcctca tcccagttgg      540

tgatgatacc gtgttcgatg gggtatttca gggtgaggat acctcttttg ctttgggctt      600

catctcctac gtatgagtcc ttttgtccca taccgaccat gactccttga tgccttgggc      660

gaccgacgat cgaggggaag acggcacggg gtgcgtcatc tcctgcgaaa ccggctttgc      720

acataccgga tccattgtct acgacaagag ccgctacatc gtcgtcacac atgttgtctt      780

ttgaggttgg acactgctca ctagacagaa aaacctcgtg ccggaccgaa tacccggtct      840

gaacgaggtt aattaaggta cccaagaagt acttagaggc ggccgcctag cataacccct      900

tggggcctct aaacgggtct tgaggggttt tttgagaaac ggccgaatac acctgttcgg      960

atccagatct cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc     1020

cctctagatc acaagtttgt acaaaaaagc aggctaagaa ggagatatac atatggcgtc     1080

taactttacc caattcgttc tggttgataa cggcggtacg ggtgacgtta ccgtagctcc     1140

gtccaacttc gccaacggtg ttgcggaatg gattagctct aacagccgct ctcaggccta     1200

caaagtcacg tgctccgttc gtcagtctag cgcgcagaat cgcaaataca ccatcaaagt     1260

tgaagtaccg aaagtcgcaa cgcagaccgt aggcggcgta gaactcccag ttgcggcctg     1320

gcgctcttac ctcaacatgg aactgactat tccgattttt gcgacgaact ccgactgcga     1380

actgattgtt aaggcaatgc agggcctgct gaaagacggt aatccgatcc catctgcaat     1440

cgctgctaac tctggcattt actaataagc ggacgcgctg ccaccgctga gcaataacta     1500

gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact     1560

atatccggca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg     1620

gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct     1680

tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga     1740

ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg     1800

gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa     1860

tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga     1920

agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg     1980

cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga     2040

tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc     2100

aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg atcgtcacgg     2160

cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc     2220

tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct     2280

gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca     2340

attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc     2400

cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt     2460

gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg     2520

ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc     2580

tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac     2640

gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct     2700

accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt     2760

tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg     2820

ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat     2880

tacccccatg aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac     2940

cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa     3000

cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga     3060

gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca     3120

gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca     3180

gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga     3240

tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac     3300

catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct     3360

tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca     3420

gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac     3480

atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt     3540

ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg     3600

cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc     3660

tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc     3720

gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc     3780

aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac     3840

tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt     3900

aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct     3960

aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc     4020

ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt     4080

ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg     4140

atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc     4200

atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa     4260

tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag     4320

gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg     4380

tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga     4440

gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag     4500

cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa     4560

gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc     4620

atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca     4680

aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg     4740

atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat     4800

aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc     4860

aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg     4920

gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg     4980

gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt     5040

gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca     5100

ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata     5160

ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac     5220

atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa     5280

gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt     5340

atcacgaggc cctttcgtct tcaagaa                                         5367


<210>  15
<211>  4764
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10303; beta actin stem loop - coat 
       protein


<220>
<221>  promoter
<222>  (38)..(56)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (60)..(67)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (68)..(362)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (362)..(369)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (527)..(821)
<223>  beta actin antisense strand gene fragment

<220>
<221>  terminator
<222>  (887)..(934)
<223>  bacteriophage T7 transcription terminator

<400>  15
ttcttagctc cggcaagcaa ttaagaactt ccgaaattaa tacgactcac tatagggagg       60

cgatcgcgca cgaggttttt ctgtctagtg agcagtgtcc aacctcaaaa gacaacatgt      120

gtgacgacga tgtagcggct cttgtcgtag acaatggatc cggtatgtgc aaagccggtt      180

tcgcaggaga tgacgcaccc cgtgccgtct tcccctcgat cgtcggtcgc ccaaggcatc      240

aaggagtcat ggtcggtatg ggacaaaagg actcatacgt aggagatgaa gcccaaagca      300

aaagaggtat cctcaccctg aaatacccca tcgaacacgg tatcatcacc aactgggatg      360

agtttaaacc ctctagctgc tttacaaagt actggttccc tttccagcgg gatgctttat      420

ctaaacgcaa tgagagaggt attcctcagg ccacatcgct tcctagttcc gctgggatcc      480

atcgttggcg gccgaagccg ccattccata gtgagttctg gcgcgcctca tcccagttgg      540

tgatgatacc gtgttcgatg gggtatttca gggtgaggat acctcttttg ctttgggctt      600

catctcctac gtatgagtcc ttttgtccca taccgaccat gactccttga tgccttgggc      660

gaccgacgat cgaggggaag acggcacggg gtgcgtcatc tcctgcgaaa ccggctttgc      720

acataccgga tccattgtct acgacaagag ccgctacatc gtcgtcacac atgttgtctt      780

ttgaggttgg acactgctca ctagacagaa aaacctcgtg ccggaccgaa tacccggtct      840

gaacgaggtt aattaaggta cccaagaagt acttagaggc ggccgcctag cataacccct      900

tggggcctct aaacgggtct tgaggggttt tttgagaaac ggccgaatac acctgttcgg      960

atccagatcc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct     1020

gcttcctaat gcaggagtcg cataagggag agcgtcgacc gatgcccttg agagccttca     1080

acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg     1140

tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg     1200

aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct     1260

tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc     1320

aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga     1380

cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc     1440

ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag     1500

gatcgctcgc ggctcttacc agcctaactt cgatcattgg accgctgatc gtcacggcga     1560

tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc gccgccctat     1620

accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa     1680

tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt     1740

cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc     1800

catctccagc agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg     1860

catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta     1920

gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc     1980

gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg     2040

gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc     2100

ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct     2160

ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc     2220

atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac     2280

ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc     2340

ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga     2400

gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct     2460

ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct     2520

cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg     2580

cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag     2640

cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat     2700

atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc     2760

gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct     2820

cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg     2880

tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc     2940

cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga     3000

aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct     3060

cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg     3120

gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag     3180

ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat     3240

cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac     3300

aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac     3360

tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc     3420

ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt     3480

tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc     3540

ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg     3600

agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca     3660

atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca     3720

cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag     3780

ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac     3840

ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc     3900

agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct     3960

agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc     4020

gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg     4080

cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc     4140

gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat     4200

tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag     4260

tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat     4320

aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg     4380

cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca     4440

cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga     4500

aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc     4560

ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata     4620

tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg     4680

ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc     4740

acgaggccct ttcgtcttca agaa                                            4764


<210>  16
<211>  5940
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10270; beta actin stem loop + coat 
       protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (372)..(666)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (782)..(1076)
<223>  beta actin antisense gene fragment

<220>
<221>  misc_feature
<222>  (1151)..(1169)
<223>  Bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (1185)..(1232)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1558)..(1576)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1649)..(2038)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (2071)..(2118)
<223>  bacteriophage T7 transcription terminator

<400>  16
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta tgtgcaaagc      480

cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg gtcgcccaag      540

gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag atgaagccca      600

aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca tcaccaactg      660

ggatgagttt aaaccctcta gctgctttac aaagtactgg ttccctttcc agcgggatgc      720

tttatctaaa cgcaatgaga gaggtattcc tcaggccaca tcgcttccta gttcggcgcg      780

cctcatccca gttggtgatg ataccgtgtt cgatggggta tttcagggtg aggatacctc      840

ttttgctttg ggcttcatct cctacgtatg agtccttttg tcccataccg accatgactc      900

cttgatgcct tgggcgaccg acgatcgagg ggaagacggc acggggtgcg tcatctcctg      960

cgaaaccggc tttgcacata ccggatccat tgtctacgac aagagccgct acatcgtcgt     1020

cacacatgtt gtcttttgag gttggacact gctcactaga cagaaaaacc tcgtgccgga     1080

ccgaataccc ggtctgaacg agggcggccg cggtacccaa gaagtactta gagttaatta     1140

aggagttcaa acatgaggat cacccatgtc gaagctccca caccctagca taaccccttg     1200

gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc     1260

cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg     1320

agcaggactg ggcggcgggc atgcatcgtc cattccgaca gcatcgccag tcactatggc     1380

gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg     1440

tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac     1500

tacgcgatca tggcgaccac acccgtcctg tggatccaga tctcgatccc gcgaaattaa     1560

tacgactcac tatagggaga ccacaacggt ttccctctag atcacaagtt tgtacaaaaa     1620

agcaggctaa gaaggagata tacatatggc gtctaacttt acccaattcg ttctggttga     1680

taacggcggt acgggtgacg ttaccgtagc tccgtccaac ttcgccaacg gtgttgcgga     1740

atggattagc tctaacagcc gctctcaggc ctacaaagtc acgtgctccg ttcgtcagtc     1800

tagcgcgcag aatcgcaaat acaccatcaa agttgaagta ccgaaagtcg caacgcagac     1860

cgtaggcggc gtagaactcc cagttgcggc ctggcgctct tacctcaaca tggaactgac     1920

tattccgatt tttgcgacga actccgactg cgaactgatt gttaaggcaa tgcagggcct     1980

gctgaaagac ggtaatccga tcccatctgc aatcgctgct aactctggca tttactaata     2040

agcggacgcg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg     2100

gtcttgaggg gttttttgct gaaaggagga actatatccg gcatgcacca ttccttgcgg     2160

cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata     2220

agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg     2280

cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag     2340

gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga     2400

cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg     2460

tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg     2520

ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca     2580

ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca     2640

ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc     2700

taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat     2760

ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc     2820

gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa     2880

cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca     2940

aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg     3000

catctcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag     3060

gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga     3120

gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt     3180

cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat     3240

gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt     3300

aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc     3360

cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat     3420

cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta     3480

cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag     3540

aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga     3600

catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt     3660

cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct     3720

gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg     3780

tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat     3840

gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga     3900

tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg     3960

cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta     4020

tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc     4080

aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag     4140

catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac     4200

caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc     4260

ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt     4320

aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc     4380

gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga     4440

cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta     4500

ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta     4560

tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga     4620

tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg     4680

cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag     4740

tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc     4800

tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact     4860

tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt     4920

cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta     4980

ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta     5040

tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc     5100

gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat     5160

agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt     5220

atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg     5280

tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca     5340

gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta     5400

agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg     5460

cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact     5520

ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg     5580

ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt     5640

actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga     5700

ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc     5760

atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa     5820

caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt     5880

attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa     5940


<210>  17
<211>  5960
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10271; beta actin stem loop + coat 
       protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (372)..(666)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (802)..(1096)
<223>  beta actin antisense gene fragment

<220>
<221>  misc_feature
<222>  (1171)..(1189)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (1205)..(1252)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1578)..(1596)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1669)..(2058)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (2091)..(2138)
<223>  bacteriophage T7 transcription terminator

<400>  17
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta tgtgcaaagc      480

cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg gtcgcccaag      540

gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag atgaagccca      600

aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca tcaccaactg      660

ggatgagttt aaaccctcta gctgctttac aaagtactgg ttccctttcc agcgggatgc      720

tttatctaaa cgcaatgaga gaggtattcc tcaggccaca tcgcttccta gttccgctgg      780

gatccatcgt tggcggcgcg cctcatccca gttggtgatg ataccgtgtt cgatggggta      840

tttcagggtg aggatacctc ttttgctttg ggcttcatct cctacgtatg agtccttttg      900

tcccataccg accatgactc cttgatgcct tgggcgaccg acgatcgagg ggaagacggc      960

acggggtgcg tcatctcctg cgaaaccggc tttgcacata ccggatccat tgtctacgac     1020

aagagccgct acatcgtcgt cacacatgtt gtcttttgag gttggacact gctcactaga     1080

cagaaaaacc tcgtgccgga ccgaataccc ggtctgaacg agggcggccg cggtacccaa     1140

gaagtactta gagttaatta aggagttcaa acatgaggat cacccatgtc gaagctccca     1200

caccctagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg     1260

aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag     1320

tggctccaag tagcgaagcg agcaggactg ggcggcgggc atgcatcgtc cattccgaca     1380

gcatcgccag tcactatggc gtgctgctag cgctatatgc gttgatgcaa tttctatgcg     1440

cacccgttct cggagcactg tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc     1500

tacttggagc cactatcgac tacgcgatca tggcgaccac acccgtcctg tggatccaga     1560

tctcgatccc gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag     1620

atcacaagtt tgtacaaaaa agcaggctaa gaaggagata tacatatggc gtctaacttt     1680

acccaattcg ttctggttga taacggcggt acgggtgacg ttaccgtagc tccgtccaac     1740

ttcgccaacg gtgttgcgga atggattagc tctaacagcc gctctcaggc ctacaaagtc     1800

acgtgctccg ttcgtcagtc tagcgcgcag aatcgcaaat acaccatcaa agttgaagta     1860

ccgaaagtcg caacgcagac cgtaggcggc gtagaactcc cagttgcggc ctggcgctct     1920

tacctcaaca tggaactgac tattccgatt tttgcgacga actccgactg cgaactgatt     1980

gttaaggcaa tgcagggcct gctgaaagac ggtaatccga tcccatctgc aatcgctgct     2040

aactctggca tttactaata agcggacgcg ctgccaccgc tgagcaataa ctagcataac     2100

cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg     2160

gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt     2220

cctaatgcag gagtcgcata agggagagcg tcgaccgatg cccttgagag ccttcaaccc     2280

agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt     2340

ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga     2400

ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca     2460

cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc     2520

cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg     2580

aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc     2640

gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc     2700

gctcgcggct cttaccagcc taacttcgat cattggaccg ctgatcgtca cggcgattta     2760

tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct     2820

tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga     2880

agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg     2940

cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc     3000

tccagcagcc gcacgcggcg catctcgggc agcgttgggt cctggccacg ggtgcgcatg     3060

atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag     3120

aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc     3180

tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag     3240

tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt     3300

ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg     3360

tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt     3420

tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc     3480

atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt     3540

aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg     3600

gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac     3660

cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg     3720

gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg     3780

tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga     3840

gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc     3900

ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt     3960

cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact     4020

caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag     4080

caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata     4140

ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc     4200

cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg     4260

ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc     4320

tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg     4380

gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc     4440

ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga     4500

ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg     4560

gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa     4620

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg     4680

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt     4740

ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat     4800

tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct     4860

aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta     4920

tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa     4980

ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac     5040

gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa     5100

gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag     5160

taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg     5220

tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag     5280

ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg     5340

tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc     5400

ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat     5460

tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata     5520

ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa     5580

aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca     5640

actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc     5700

aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc     5760

tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg     5820

aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac     5880

ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga     5940

ggccctttcg tcttcaagaa                                                 5960


<210>  18
<211>  5980
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10272; beta actin stem loop + coat 
       protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_features
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_features
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_features
<222>  (372)..(666)
<223>  beta actin sense gene fragment

<220>
<221>  misc_features
<222>  (822)..(1116)
<223>  beta actin antisense gene fragment

<220>
<221>  misc_feature
<222>  (1191)..(1209)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (1225)..(1272)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1598)..(1616)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1689)..(2078)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (2111)..(2158)
<223>  bacteriophage T7 transcription terminator

<400>  18
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta tgtgcaaagc      480

cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg gtcgcccaag      540

gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag atgaagccca      600

aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca tcaccaactg      660

ggatgagttt aaaccctcta gctgctttac aaagtactgg ttccctttcc agcgggatgc      720

tttatctaaa cgcaatgaga gaggtattcc tcaggccaca tcgcttccta gttccgctgg      780

gatccatcgt tggcggccga agccgccatt ccatggcgcg cctcatccca gttggtgatg      840

ataccgtgtt cgatggggta tttcagggtg aggatacctc ttttgctttg ggcttcatct      900

cctacgtatg agtccttttg tcccataccg accatgactc cttgatgcct tgggcgaccg      960

acgatcgagg ggaagacggc acggggtgcg tcatctcctg cgaaaccggc tttgcacata     1020

ccggatccat tgtctacgac aagagccgct acatcgtcgt cacacatgtt gtcttttgag     1080

gttggacact gctcactaga cagaaaaacc tcgtgccgga ccgaataccc ggtctgaacg     1140

agggcggccg cggtacccaa gaagtactta gagttaatta aggagttcaa acatgaggat     1200

cacccatgtc gaagctccca caccctagca taaccccttg gggcctctaa acgggtcttg     1260

aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc     1320

catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcgggc     1380

atgcatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc     1440

gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg     1500

cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac     1560

acccgtcctg tggatccaga tctcgatccc gcgaaattaa tacgactcac tatagggaga     1620

ccacaacggt ttccctctag atcacaagtt tgtacaaaaa agcaggctaa gaaggagata     1680

tacatatggc gtctaacttt acccaattcg ttctggttga taacggcggt acgggtgacg     1740

ttaccgtagc tccgtccaac ttcgccaacg gtgttgcgga atggattagc tctaacagcc     1800

gctctcaggc ctacaaagtc acgtgctccg ttcgtcagtc tagcgcgcag aatcgcaaat     1860

acaccatcaa agttgaagta ccgaaagtcg caacgcagac cgtaggcggc gtagaactcc     1920

cagttgcggc ctggcgctct tacctcaaca tggaactgac tattccgatt tttgcgacga     1980

actccgactg cgaactgatt gttaaggcaa tgcagggcct gctgaaagac ggtaatccga     2040

tcccatctgc aatcgctgct aactctggca tttactaata agcggacgcg ctgccaccgc     2100

tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct     2160

gaaaggagga actatatccg gcatgcacca ttccttgcgg cggcggtgct caacggcctc     2220

aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg     2280

cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc     2340

gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc     2400

tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt     2460

gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa     2520

cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc     2580

ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc     2640

ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat     2700

cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cattggaccg     2760

ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt     2820

gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg     2880

gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa     2940

ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca     3000

tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc agcgttgggt     3060

cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg     3120

gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct     3180

gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt     3240

aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag     3300

gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac     3360

cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac     3420

gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt     3480

tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacca     3540

aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc     3600

tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg     3660

accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc     3720

tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca     3780

gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc     3840

agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt     3900

actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg     3960

catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg     4020

gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa     4080

cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc     4140

gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc     4200

aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag     4260

ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct     4320

cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta     4380

ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc     4440

cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc     4500

agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt     4560

gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct     4620

gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc     4680

tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca     4740

agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta     4800

agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa     4860

atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg     4920

cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg     4980

actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc     5040

aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc     5100

cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa     5160

ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc     5220

cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg     5280

ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc     5340

cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat     5400

ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg     5460

tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc     5520

ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg     5580

aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat     5640

gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg     5700

gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg     5760

ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct     5820

catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac     5880

atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta     5940

taaaaatagg cgtatcacga ggccctttcg tcttcaagaa                           5980


<210>  19
<211>  6005
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10292; beta actin stem loop + coat 
       protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (372)..(666)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (797)..(1091)
<223>  beta actin antisense gene fragment

<220>
<221>  misc_feature
<222>  (1166)..(1184)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (1200)..(1247)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1573)..(1591)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1664)..(2053)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (2086)..(2133)
<223>  bacteriophage T7 transcription terminator

<400>  19
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta tgtgcaaagc      480

cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg gtcgcccaag      540

gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag atgaagccca      600

aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca tcaccaactg      660

ggatgagttt aaacgcaatc gcagcaaact ccggcatcta ctaatagacg ccggccattc      720

aacatgagga ttacccatgt aacctaagaa gacaacaaag aagttcaact ctttatgtat      780

tgatcttccg gcgcgcctca tcccagttgg tgatgatacc gtgttcgatg gggtatttca      840

gggtgaggat acctcttttg ctttgggctt catctcctac gtatgagtcc ttttgtccca      900

taccgaccat gactccttga tgccttgggc gaccgacgat cgaggggaag acggcacggg      960

gtgcgtcatc tcctgcgaaa ccggctttgc acataccgga tccattgtct acgacaagag     1020

ccgctacatc gtcgtcacac atgttgtctt ttgaggttgg acactgctca ctagacagaa     1080

aaacctcgtg ccggaccgaa tacccggtct gaacgagggc ggccgcggta cccaagaagt     1140

acttagagtt aattaaggag ttcaaacatg aggatcaccc atgtcgaagc tcccacaccc     1200

tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa     1260

ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct     1320

ccaagtagcg aagcgagcag gactgggcgg cgggcatgca tcgtccattc cgacagcatc     1380

gccagtcact atggcgtgct gctagcgcta tatgcgttga tgcaatttct atgcgcaccc     1440

gttctcggag cactgtccga ccgctttggc cgccgcccag tcctgctcgc ttcgctactt     1500

ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat ccagatctcg     1560

atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc tctagatcac     1620

aagtttgtac aaaaaagcag gctaagaagg agatatacat atggcgtcta actttaccca     1680

attcgttctg gttgataacg gcggtacggg tgacgttacc gtagctccgt ccaacttcgc     1740

caacggtgtt gcggaatgga ttagctctaa cagccgctct caggcctaca aagtcacgtg     1800

ctccgttcgt cagtctagcg cgcagaatcg caaatacacc atcaaagttg aagtaccgaa     1860

agtcgcaacg cagaccgtag gcggcgtaga actcccagtt gcggcctggc gctcttacct     1920

caacatggaa ctgactattc cgatttttgc gacgaactcc gactgcgaac tgattgttaa     1980

ggcaatgcag ggcctgctga aagacggtaa tccgatccca tctgcaatcg ctgctaactc     2040

tggcatttac taataagcgg acgcgctgcc accgctgagc aataactagc ataacccctt     2100

ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggcatg     2160

caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa     2220

tgcaggagtc gcataaggga gagcgtcgac cgatgccctt gagagccttc aacccagtca     2280

gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta     2340

tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct     2400

ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc     2460

tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta     2520

tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct     2580

ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc     2640

aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg     2700

cggctcttac cagcctaact tcgatcattg gaccgctgat cgtcacggcg atttatgccg     2760

cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct     2820

gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg     2880

gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag     2940

aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag     3000

cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt     3060

gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga     3120

atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc     3180

aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc     3240

gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac     3300

acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg     3360

ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc     3420

atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa     3480

cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat     3540

ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc     3600

ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag     3660

ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac     3720

ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc     3780

gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta     3840

tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt     3900

gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg     3960

ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag     4020

gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa     4080

ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc     4140

cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca     4200

ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg     4260

accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct     4320

catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt     4380

gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag     4440

tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc     4500

agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac     4560

actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga     4620

gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc     4680

aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg     4740

gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca     4800

aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt     4860

atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca     4920

gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg     4980

atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca     5040

ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt     5100

cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt     5160

agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca     5220

cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca     5280

tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga     5340

agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact     5400

gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga     5460

gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg     5520

ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc     5580

tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga     5640

tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat     5700

gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt     5760

caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt     5820

atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac     5880

gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc     5940

tttcgtcttc aagaacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc     6000

aagaa                                                                 6005


<210>  20
<211>  5966
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10291; beta actin stem loop + coat 
       protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (372)..(666)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (808)..(1102)
<223>  beta actin antisense gene fragment

<220>
<221>  misc_feature
<222>  (1177)..(1195)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (1211)..(1258)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1584)..(1602)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1675)..(2064)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (2097)..(2144)
<223>  bacteriophage T7 transcription terminator

<400>  20
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta tgtgcaaagc      480

cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg gtcgcccaag      540

gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag atgaagccca      600

aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca tcaccaactg      660

ggatgagttt aaacttaagc ggaacaccag gcggaacgaa gaggagatag agaactagat      720

tgattagaat caaatactag aactactaaa tcgaatcgat acgctaacga aaggacctgg      780

acacgtcgac gagccgctgg ggcgcgcctc atcccagttg gtgatgatac cgtgttcgat      840

ggggtatttc agggtgagga tacctctttt gctttgggct tcatctccta cgtatgagtc      900

cttttgtccc ataccgacca tgactccttg atgccttggg cgaccgacga tcgaggggaa      960

gacggcacgg ggtgcgtcat ctcctgcgaa accggctttg cacataccgg atccattgtc     1020

tacgacaaga gccgctacat cgtcgtcaca catgttgtct tttgaggttg gacactgctc     1080

actagacaga aaaacctcgt gccggaccga atacccggtc tgaacgaggg cggccgcggt     1140

acccaagaag tacttagagt taattaagga gttcaaacat gaggatcacc catgtcgaag     1200

ctcccacacc ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct     1260

gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt     1320

cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcgggcatgc atcgtccatt     1380

ccgacagcat cgccagtcac tatggcgtgc tgctagcgct atatgcgttg atgcaatttc     1440

tatgcgcacc cgttctcgga gcactgtccg accgctttgg ccgccgccca gtcctgctcg     1500

cttcgctact tggagccact atcgactacg cgatcatggc gaccacaccc gtcctgtgga     1560

tccagatctc gatcccgcga aattaatacg actcactata gggagaccac aacggtttcc     1620

ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca tatggcgtct     1680

aactttaccc aattcgttct ggttgataac ggcggtacgg gtgacgttac cgtagctccg     1740

tccaacttcg ccaacggtgt tgcggaatgg attagctcta acagccgctc tcaggcctac     1800

aaagtcacgt gctccgttcg tcagtctagc gcgcagaatc gcaaatacac catcaaagtt     1860

gaagtaccga aagtcgcaac gcagaccgta ggcggcgtag aactcccagt tgcggcctgg     1920

cgctcttacc tcaacatgga actgactatt ccgatttttg cgacgaactc cgactgcgaa     1980

ctgattgtta aggcaatgca gggcctgctg aaagacggta atccgatccc atctgcaatc     2040

gctgctaact ctggcattta ctaataagcg gacgcgctgc caccgctgag caataactag     2100

cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta     2160

tatccggcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg     2220

ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt     2280

caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac     2340

tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg     2400

cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat     2460

cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa     2520

gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc     2580

gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat     2640

gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca     2700

aggatcgctc gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc     2760

gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct     2820

ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg     2880

aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa     2940

ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc     3000

gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg     3060

cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt     3120

tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct     3180

gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg     3240

cggaagtcag cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta     3300

ccctgtggaa cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt     3360

ctctggtccc gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg     3420

gcatgttcat catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt     3480

acccccatga acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc     3540

gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac     3600

gagctggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag     3660

ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag     3720

ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag     3780

ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat     3840

agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc     3900

atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt     3960

ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag     4020

ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca     4080

tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt     4140

tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc     4200

gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct     4260

ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg     4320

tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca     4380

agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact     4440

atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta     4500

acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta     4560

actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct     4620

tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt     4680

tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga     4740

tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca     4800

tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat     4860

caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg     4920

cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt     4980

agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag     5040

acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc     5100

gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag     5160

ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca     5220

tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa     5280

ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga     5340

tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata     5400

attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca     5460

agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg     5520

ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg     5580

ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg     5640

cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag     5700

gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac     5760

tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca     5820

tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag     5880

tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta     5940

tcacgaggcc ctttcgtctt caagaa                                          5966


<210>  21
<211>  5502
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10276; beta actin stem loop + coat 
       protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (372)..(422)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (588)..(638)
<223>  beta actin antisense gene fragment

<220>
<221>  misc_feature
<222>  (713)..(731)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (747)..(794)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1120)..(1138)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1211)..(1600)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (1633)..(1680)
<223>  bacteriophage T7 transcription terminator

<400>  21
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

cagtttaaac cctctagctg ctttacaaag tactggttcc ctttccagcg ggatgcttta      480

tctaaacgca atgagagagg tattcctcag gccacatcgc ttcctagttc cgctgggatc      540

catcgttggc ggccgaagcc gccattccat agtgagttct ggcgcgcctg ttgtcttttg      600

aggttggaca ctgctcacta gacagaaaaa cctcgtgccg gaccgaatac ccggtctgaa      660

cgagggcggc cgcggtaccc aagaagtact tagagttaat taaggagttc aaacatgagg      720

atcacccatg tcgaagctcc cacaccctag cataacccct tggggcctct aaacgggtct      780

tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc      840

gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg      900

gcatgcatcg tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat      960

gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc     1020

cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc     1080

acacccgtcc tgtggatcca gatctcgatc ccgcgaaatt aatacgactc actataggga     1140

gaccacaacg gtttccctct agatcacaag tttgtacaaa aaagcaggct aagaaggaga     1200

tatacatatg gcgtctaact ttacccaatt cgttctggtt gataacggcg gtacgggtga     1260

cgttaccgta gctccgtcca acttcgccaa cggtgttgcg gaatggatta gctctaacag     1320

ccgctctcag gcctacaaag tcacgtgctc cgttcgtcag tctagcgcgc agaatcgcaa     1380

atacaccatc aaagttgaag taccgaaagt cgcaacgcag accgtaggcg gcgtagaact     1440

cccagttgcg gcctggcgct cttacctcaa catggaactg actattccga tttttgcgac     1500

gaactccgac tgcgaactga ttgttaaggc aatgcagggc ctgctgaaag acggtaatcc     1560

gatcccatct gcaatcgctg ctaactctgg catttactaa taagcggacg cgctgccacc     1620

gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg     1680

ctgaaaggag gaactatatc cggcatgcac cattccttgc ggcggcggtg ctcaacggcc     1740

tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaccga     1800

tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg     1860

tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc     1920

tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc     1980

ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca     2040

aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg     2100

tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt     2160

ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc     2220

atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcattggac     2280

cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga     2340

ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc     2400

gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag     2460

aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa     2520

catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg     2580

gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg     2640

gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg     2700

ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc     2760

gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc     2820

aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg     2880

accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca     2940

acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg     3000

tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac     3060

caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct     3120

tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca     3180

cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa     3240

cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag     3300

cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac     3360

ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt     3420

gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac     3480

cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg     3540

cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat     3600

aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc     3660

gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc     3720

tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga     3780

agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt     3840

ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg     3900

taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc     3960

gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg     4020

gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc     4080

ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg     4140

ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc     4200

gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct     4260

caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt     4320

taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa     4380

aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa     4440

tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc     4500

tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct     4560

gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca     4620

gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt     4680

aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt     4740

gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc     4800

ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc     4860

tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt     4920

atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact     4980

ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc     5040

ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt     5100

ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg     5160

atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct     5220

gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa     5280

tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt     5340

ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc     5400

acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc     5460

tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aa                        5502


<210>  22
<211>  5552
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10277; beta actin stem loop + coat 
       protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (372)..(447)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (613)..(688)
<223>  beta actin antisense gene fragment

<220>
<221>  misc_feature
<222>  (763)..(781)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (797)..(844)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1170)..(1188)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1261)..(1650)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (1683)..(1730)
<223>  bacteriophage T7 transcription terminator

<400>  22
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

catgtgtgac gacgatgtag cggctctgtt taaaccctct agctgcttta caaagtactg      480

gttccctttc cagcgggatg ctttatctaa acgcaatgag agaggtattc ctcaggccac      540

atcgcttcct agttccgctg ggatccatcg ttggcggccg aagccgccat tccatagtga      600

gttctggcgc gccagagccg ctacatcgtc gtcacacatg ttgtcttttg aggttggaca      660

ctgctcacta gacagaaaaa cctcgtgccg gaccgaatac ccggtctgaa cgagggcggc      720

cgcggtaccc aagaagtact tagagttaat taaggagttc aaacatgagg atcacccatg      780

tcgaagctcc cacaccctag cataacccct tggggcctct aaacgggtct tgaggggttt      840

tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg      900

cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg gcatgcatcg      960

tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc     1020

aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc     1080

tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc     1140

tgtggatcca gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg     1200

gtttccctct agatcacaag tttgtacaaa aaagcaggct aagaaggaga tatacatatg     1260

gcgtctaact ttacccaatt cgttctggtt gataacggcg gtacgggtga cgttaccgta     1320

gctccgtcca acttcgccaa cggtgttgcg gaatggatta gctctaacag ccgctctcag     1380

gcctacaaag tcacgtgctc cgttcgtcag tctagcgcgc agaatcgcaa atacaccatc     1440

aaagttgaag taccgaaagt cgcaacgcag accgtaggcg gcgtagaact cccagttgcg     1500

gcctggcgct cttacctcaa catggaactg actattccga tttttgcgac gaactccgac     1560

tgcgaactga ttgttaaggc aatgcagggc ctgctgaaag acggtaatcc gatcccatct     1620

gcaatcgctg ctaactctgg catttactaa taagcggacg cgctgccacc gctgagcaat     1680

aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag     1740

gaactatatc cggcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact     1800

actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaccga tgcccttgag     1860

agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact     1920

tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat     1980

tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt     2040

cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg     2100

cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc     2160

gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat     2220

cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca     2280

gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt     2340

cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc     2400

cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc     2460

gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc     2520

aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa catatccatc     2580

gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca     2640

cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt     2700

actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa     2760

acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg     2820

gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc     2880

tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg     2940

atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt     3000

aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt     3060

atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa     3120

aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa     3180

ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct     3240

gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac     3300

atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc     3360

cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt     3420

agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag     3480

tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc     3540

gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg     3600

tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa     3660

agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg     3720

cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga     3780

ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg     3840

tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg     3900

gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc     3960

gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg     4020

gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca     4080

ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt     4140

ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag     4200

ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg     4260

gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc     4320

ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt     4380

tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt     4440

ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca     4500

gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg     4560

tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac     4620

cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg     4680

ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc     4740

gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg     4800

caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac     4860

gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc     4920

ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac     4980

tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact     5040

caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa     5100

cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt     5160

cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca     5220

ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa     5280

aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac     5340

tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg     5400

gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc     5460

gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata     5520

ggcgtatcac gaggcccttt cgtcttcaag aa                                   5552


<210>  23
<211>  4667
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10149; coat protein


<220>
<221>  promoter
<222>  (1896)..(1914)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1987)..(2376)
<223>  bacteriophage MS2 coat protein

<220>
<221>  terminator
<222>  (2409)..(2456)
<223>  bacteriophage T7 transcription terminator

<400>  23
gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt       60

gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt      120

ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga      180

tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga      240

aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt      300

ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc      360

ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat      420

ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt      480

gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg      540

acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact      600

ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa      660

aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc      720

actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc      780

ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa      840

agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc      900

agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc      960

tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc     1020

gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac     1080

tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt     1140

gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt     1200

agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg     1260

tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt     1320

cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc     1380

aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca     1440

atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc     1500

atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct     1560

aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg     1620

tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg     1680

atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt     1740

tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc     1800

cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac     1860

ccgtcctgtg gatccagatc tcgatcccgc gaaattaata cgactcacta tagggagacc     1920

acaacggttt ccctctagat cacaagtttg tacaaaaaag caggctaaga aggagatata     1980

catatggcgt ctaactttac ccaattcgtt ctggttgata acggcggtac gggtgacgtt     2040

accgtagctc cgtccaactt cgccaacggt gttgcggaat ggattagctc taacagccgc     2100

tctcaggcct acaaagtcac gtgctccgtt cgtcagtcta gcgcgcagaa tcgcaaatac     2160

accatcaaag ttgaagtacc gaaagtcgca acgcagaccg taggcggcgt agaactccca     2220

gttgcggcct ggcgctctta cctcaacatg gaactgacta ttccgatttt tgcgacgaac     2280

tccgactgcg aactgattgt taaggcaatg cagggcctgc tgaaagacgg taatccgatc     2340

ccatctgcaa tcgctgctaa ctctggcatt tactaataag cggacgcgct gccaccgctg     2400

agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga     2460

aaggaggaac tatatccggc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa     2520

cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaccgatgcc     2580

cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc     2640

cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg     2700

ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc     2760

ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg     2820

tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt     2880

gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg     2940

cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca     3000

gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca ttggaccgct     3060

gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt     3120

aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc     3180

cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac tccaagaatt     3240

ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg gcagaacata     3300

tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag cgttgggtcc     3360

tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt     3420

tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc     3480

tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa     3540

agtctggaaa cgcggaagtc ccctacgtgc tgctgaagtt gcccgcaaca gagagtggaa     3600

ccaaccggtg ataccacgat actatgactg agagtcaacg ccatgagcgg cctcatttct     3660

tattctgagt tacaacagtc cgcaccgctg tccggtagct ccttccggtg ggcgcggggc     3720

atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg     3780

ccggcagcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa     3840

gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga     3900

acacctacat ctgtattaac gaagcgctaa ccgtttttat caggctctgg gaggcagaat     3960

aaatgatcat atcgtcaatt attacctcca cggggagagc ctgagcaaac tggcctcagg     4020

catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat     4080

agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt     4140

cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcacc aggcgtttaa     4200

gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt     4260

tgtaattcat taagcattct gccgacatgg aagccatcac agacggcatg atgaacctga     4320

atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg     4380

ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag     4440

ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt     4500

tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg     4560

tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg     4620

tgaacactat cccatatcac cagctcaccg tctttcattg ccatacg                   4667


<210>  24
<211>  6228
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10366; beta actin stem loop + eGFP 
       protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (372)..(666)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (832)..(1126)
<223>  beta actin antisense gene fragment

<220>
<221>  misc_feature
<222>  (1201)..(1219)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (1235)..(1282)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1608)..(1626)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1696)..(2415)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (2448)..(2495)
<223>  bacteriophage T7 transcription terminator

<400>  24
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta tgtgcaaagc      480

cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg gtcgcccaag      540

gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag atgaagccca      600

aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca tcaccaactg      660

ggatgagttt aaaccctcta gctgctttac aaagtactgg ttccctttcc agcgggatgc      720

tttatctaaa cgcaatgaga gaggtattcc tcaggccaca tcgcttccta gttccgctgg      780

gatccatcgt tggcggccga agccgccatt ccatagtgag ttctggcgcg cctcatccca      840

gttggtgatg ataccgtgtt cgatggggta tttcagggtg aggatacctc ttttgctttg      900

ggcttcatct cctacgtatg agtccttttg tcccataccg accatgactc cttgatgcct      960

tgggcgaccg acgatcgagg ggaagacggc acggggtgcg tcatctcctg cgaaaccggc     1020

tttgcacata ccggatccat tgtctacgac aagagccgct acatcgtcgt cacacatgtt     1080

gtcttttgag gttggacact gctcactaga cagaaaaacc tcgtgccgga ccgaataccc     1140

ggtctgaacg agggcggccg cggtacccaa gaagtactta gagttaatta aggagttcaa     1200

acatgaggat cacccatgtc gaagctccca caccctagca taaccccttg gggcctctaa     1260

acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg     1320

gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg     1380

ggcggcgggc atgcatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag     1440

cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct     1500

ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca     1560

tggcgaccac acccgtcctg tggatccaga tctcgatccc gcgaaattaa tacgactcac     1620

tatagggaga ccacaacggt ttccctctag atcacaagtt tgtacaaaaa agcaggctaa     1680

gaaggagata tacatatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc     1740

ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag     1800

ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc     1860

gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac     1920

cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag     1980

gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc     2040

gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc     2100

aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc     2160

gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc     2220

agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg     2280

ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag     2340

cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac     2400

gagctgtaca agtaataagc ggacgcgctg ccaccgctga gcaataacta gcataacccc     2460

ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggtc     2520

gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga     2580

ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg     2640

cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc     2700

tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg     2760

ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg     2820

gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc     2880

tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg     2940

acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca     3000

ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg     3060

catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat     3120

ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac     3180

tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg     3240

gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag     3300

cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg     3360

ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag     3420

cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg     3480

tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg     3540

catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg     3600

gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc     3660

ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct     3720

ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc     3780

agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt     3840

aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc     3900

gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg     3960

tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc     4020

cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc     4080

catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag     4140

cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga     4200

aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt     4260

cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca     4320

ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa     4380

aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat     4440

cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc     4500

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc     4560

gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt     4620

tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac     4680

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg     4740

ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca     4800

gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc     4860

gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa     4920

accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa     4980

ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac     5040

tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta     5100

aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt     5160

taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata     5220

gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc     5280

agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac     5340

cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag     5400

tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac     5460

gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc     5520

agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg     5580

gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc     5640

atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct     5700

gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc     5760

tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc     5820

atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc     5880

agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc     5940

gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca     6000

cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt     6060

tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt     6120

ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca     6180

ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaa                  6228


<210>  25
<211>  5499
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10359; beta actin stem loop + Qbeta coat
       protein


<220>
<221>  promoter
<222>  (24)..(42)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (46)..(65)
<223>  bacteriophage Qbeta pac sequence

<220>
<221>  misc_feature
<222>  (66)..(73)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (74)..(367)
<223>  beta actin sense strand gene fragment

<220>
<221>  misc_feature
<222>  (534)..(827)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (872)..(891)
<223>  bacteriophage Qbeta pac sequence

<220>
<221>  terminator
<222>  (907)..(954)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1108)..(1126)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1196)..(1597)
<223>  bacteriophage Qbeta coat protein

<220>
<221>  terminator
<222>  (1630)..(1677)
<223>  bacteriophage T7 transcription terminator

<400>  25
ttcagatctc gatcccgcga aattaatacg actcactata gggagatgca tgtctaagac       60

agcatgcgat cgcgcacgag gtttttctgt ctagtgagca gtgtccaacc tcaaaagaca      120

acatgtgtga cgacgatgta gcggctcttg tcgtagacaa tggatccggt atgtgcaaag      180

ccggtttcgc aggagatgac gcaccccgtg ccgtcttccc ctcgatcgtc ggtcgcccaa      240

ggcatcaagg agtcatggtc ggtatgggac aaaaggactc atacgtagga gatgaagccc      300

aaagcaaaag aggtatcctc accctgaaat accccatcga acacggtatc atcaccaact      360

gggatgagtt taaaccctct agctgcttta caaagtactg gttccctttc cagcgggatg      420

ctttatctaa acgcaatgag agaggtattc ctcaggccac atcgcttcct agttccgctg      480

ggatccatcg ttggcggccg aagccgccat tccatagtga gttctggcgc gcctcatccc      540

agttggtgat gataccgtgt tcgatggggt atttcagggt gaggatacct cttttgcttt      600

gggcttcatc tcctacgtat gagtcctttt gtcccatacc gaccatgact ccttgatgcc      660

ttgggcgacc gacgatcgag gggaagacgg cacggggtgc gtcatctcct gcgaaaccgg      720

ctttgcacat accggatcca ttgtctacga caagagccgc tacatcgtcg tcacacatgt      780

tgtcttttga ggttggacac tgctcactag acagaaaaac ctcgtgccgg accgaatacc      840

cggtctgaac gagggcggcc gcggagttca aatgcatgtc taagacagca tcgaagctcc      900

cacaccctag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa      960

ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat     1020

agtggctcca agtagcgaag cgagcaggac tgggcggcgg gcatgcgcct ccgcctttag     1080

gggatccaga tctcgatccc gcgaaattaa tacgactcac tatagggaga ccacaacggt     1140

ttccctctag atcacaagtt tgtacaaaaa agcaggctaa gaaggagata tacatatggc     1200

aaaattagag actgttactt taggtaacat cgggaaagat ggaaaacaaa ctctggtcct     1260

caatccgcgt ggggtaaatc ccactaacgg cgttgcctcg ctttcacaag cgggtgcagt     1320

tcctgcgctg gagaagcgtg ttaccgtttc ggtatctcag ccttctcgca atcgtaagaa     1380

ctacaaggtc caggttaaga tccagaaccc gaccgcttgc actgcaaacg gttcttgtga     1440

cccatccgtt actcgccagg catatgctga cgtgaccttt tcgttcacgc agtatagtac     1500

cgatgaggaa cgagcttttg ttcgtacaga gcttactgct ctgctcgcta gtcctctgct     1560

gatcgatgct attgatcagc tgaacccagc gtattaataa gcggacgcgc tgccaccgct     1620

gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg     1680

aaaggaggaa ctatatccgg catgcaccat tccttgcggc ggcggtgctc aacggcctca     1740

acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc     1800

ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg     1860

ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct     1920

gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg     1980

cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac     2040

gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct     2100

tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg     2160

gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc     2220

agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc attggaccgc     2280

tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg     2340

taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg     2400

ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat     2460

tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat     2520

atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc     2580

ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg     2640

ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg     2700

ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta     2760

aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg     2820

atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc     2880

ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg     2940

ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt     3000

catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa     3060

acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat taacgcttct     3120

ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga     3180

ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct     3240

ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag     3300

acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca     3360

gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta     3420

ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc     3480

atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg     3540

cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac     3600

gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg     3660

ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca     3720

agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc     3780

tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc     3840

ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag     3900

gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc     3960

ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca     4020

gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg     4080

aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg     4140

aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct     4200

ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa     4260

gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa     4320

gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa     4380

tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc     4440

ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga     4500

ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca     4560

atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc     4620

ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat     4680

tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc     4740

attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt     4800

tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc     4860

ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg     4920

gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt     4980

gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg     5040

gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga     5100

aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg     5160

taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg     5220

tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt     5280

tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc     5340

atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca     5400

tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat     5460

aaaaataggc gtatcacgag gccctttcgt cttcaagaa                            5499


<210>  26
<211>  5413
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10357; beta actin stem loop + U1A 
       protein


<220>
<221>  promoter
<222>  (24)..(42)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (41)..(66)
<223>  U1A binding site sequence

<220>
<221>  misc_feature
<222>  (67)..(74)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (75)..(368)
<223>  beta actin sense strand gene fragment

<220>
<221>  misc_feature
<222>  (535)..(828)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (873)..(898)
<223>  U1A binding site sequence

<220>
<221>  terminator
<222>  (914)..(961)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1115)..(1133)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1203)..(1511)
<223>  human U1A protein gene

<220>
<221>  terminator
<222>  (1544)..(1591)
<223>  bacteriophage T7 transcription terminator

<400>  26
ttcagatctc gatcccgcga aattaatacg actcactata gggtatccat tgcactccgg       60

atgcccgcga tcgcgcacga ggtttttctg tctagtgagc agtgtccaac ctcaaaagac      120

aacatgtgtg acgacgatgt agcggctctt gtcgtagaca atggatccgg tatgtgcaaa      180

gccggtttcg caggagatga cgcaccccgt gccgtcttcc cctcgatcgt cggtcgccca      240

aggcatcaag gagtcatggt cggtatggga caaaaggact catacgtagg agatgaagcc      300

caaagcaaaa gaggtatcct caccctgaaa taccccatcg aacacggtat catcaccaac      360

tgggatgagt ttaaaccctc tagctgcttt acaaagtact ggttcccttt ccagcgggat      420

gctttatcta aacgcaatga gagaggtatt cctcaggcca catcgcttcc tagttccgct      480

gggatccatc gttggcggcc gaagccgcca ttccatagtg agttctggcg cgcctcatcc      540

cagttggtga tgataccgtg ttcgatgggg tatttcaggg tgaggatacc tcttttgctt      600

tgggcttcat ctcctacgta tgagtccttt tgtcccatac cgaccatgac tccttgatgc      660

cttgggcgac cgacgatcga ggggaagacg gcacggggtg cgtcatctcc tgcgaaaccg      720

gctttgcaca taccggatcc attgtctacg acaagagccg ctacatcgtc gtcacacatg      780

ttgtcttttg aggttggaca ctgctcacta gacagaaaaa cctcgtgccg gaccgaatac      840

ccggtctgaa cgagggcggc cgcggagttc aagggtatcc attgcactcc ggatgccccg      900

aagctcccac accctagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt      960

gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt     1020

agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcgggca tgcgcctccg     1080

cctttagggg atccagatct cgatcccgcg aaattaatac gactcactat agggagacca     1140

caacggtttc cctctagatc acaagtttgt acaaaaaagc aggctaagaa ggagatatac     1200

atatggcagt tcccgagacc cgccctaacc acactattta tatcaacaac ctcaatgaga     1260

agatcaagaa ggatgagcta aaaaagtccc tgtacgccat cttctcccag tttggccaga     1320

tcctggatat cctggtatca cggagcctga agatgagggg ccaggccttt gtcatcttca     1380

aggaggtcag cagcgccacc aacgccctgc gctccatgca gggtttccct ttctatgaca     1440

aacctatgcg tatccagtat gccaagaccg actcagatat cattgccaag atgaaaggca     1500

ccttcgtgta ataagcggac gcgctgccac cgctgagcaa taactagcat aaccccttgg     1560

ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggcatgca     1620

ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg     1680

caggagtcgc ataagggaga gcgtcgaccg atgcccttga gagccttcaa cccagtcagc     1740

tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc     1800

atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt     1860

cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc     1920

gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc     1980

gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg     2040

atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag     2100

gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg     2160

gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc     2220

tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc     2280

ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc     2340

ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa     2400

ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca     2460

gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc     2520

tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat     2580

caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa     2640

caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc     2700

cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac     2760

ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc     2820

gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat     2880

cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca     2940

gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg     3000

cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg     3060

atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct     3120

gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg     3180

tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg     3240

gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata     3300

ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga     3360

aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct     3420

cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc     3480

ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg     3540

ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg     3600

cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg     3660

actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac     3720

cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca     3780

tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt     3840

gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc     3900

caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag     3960

agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac     4020

tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt     4080

tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa     4140

gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg     4200

gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa     4260

aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat     4320

atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc     4380

gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat     4440

acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc     4500

ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc     4560

tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag     4620

ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg     4680

ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg     4740

atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag     4800

taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt     4860

catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga     4920

atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc     4980

acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc     5040

aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc     5100

ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc     5160

cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca     5220

atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat     5280

ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt     5340

ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt     5400

tcgtcttcaa gaa                                                        5413


<210>  27
<211>  5504
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10372; beta actin stem loop + truncated 
       coat protein


<220>
<221>  promoter
<222>  (24)..(42)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (127)..(145)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (175)..(182)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (179)..(384)
<223>  ErkA sense strand gene fragment

<220>
<221>  misc_feature
<222>  (436)..(454)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (504)..(511)
<223>  restriction endonuclease NotI recognition site

<220>
<221>  misc_feature
<222>  (505)..(696)
<223>  ErkA antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (692)..(699)
<223>  restriction endonuclease PacI recognition site

<220>
<221>  misc_feature
<222>  (728)..(746)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (762)..(809)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1135)..(1153)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (1223)..(1429)
<223>  bacteriophage MS2 coat protein N-terminal fragment

<220>
<221>  terminator
<222>  (1635)..(1682)
<223>  bacteriophage T7 transcription terminator

<400>  27
ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac aacggtttcc       60

ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca tacgccggcc      120

attcaaacat gaggattacc catgtaacct aaggccggtg tccaggcgcg ctccgcgatc      180

gcacgcggac aaagttcctc aatctaatgc tgaagttata aggggacaaa tatttgaagt      240

tggtcctagg tatattaaac tcgcctatat aggtgaagga gcttatggca tggttgtgtc      300

tgcggatgac acgctaacaa accaaagagt tgcaataaaa aaaatatcgc cctttgaaca      360

ccaaacttat tgctactaca gtttaaacgc aatcgcagca aactccggca tctactaata      420

gacgccggcc attcaacatg aggattaccc atgtaaccta agaagacaac aaagaagttc      480

aactctttat gtattgatct tccgcggccg cggtaccggg gcaataagtt tggtgttcaa      540

agggcgatat tttttttatt gcaactcttt ggtttgttag cgtgtcatcc gcagacacaa      600

ccatgccata agctccttca cctatatagg cgagtttaat atacctagga ccaacttcaa      660

atatttgtcc ccttataact tcagcattag attgaggaac tatacgaaaa ttaattaagg      720

agttcaaaca tgaggatcac ccatgtcgaa gctcccacac cctagcataa ccccttgggg      780

cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac      840

aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc      900

aggactgggc ggcgggcatg catcgtccat tccgacagca tcgccagtca ctatggcgtg      960

ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc     1020

gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac     1080

gcgatcatgg cgaccacacc cgtcctgtgg atccagatct cgatcccgcg aaattaatac     1140

gactcactat agggagacca caacggtttc cctctagatc acaagtttgt acaaaaaagc     1200

aggctaagaa ggagatatac atatggcgtc taactttacc caattcgttc tggttgataa     1260

cggcggtacg ggtgacgtta ccgtagctcc gtccaacttc gccaacggtg ttgcggaatg     1320

gattagctct aacagccgct ctcagggtgc tccgttcgtc agtctagcgc gcagaatcgc     1380

aaatacacca tcaaagttga agtaccgaaa gtcgcaacgc agaccgtagg cggcgtagaa     1440

ctcccagttg cggcctggcg ctcttacctc aacatggaac tgactattcc gatttttgcg     1500

acgaactccg actgcgaact gattgttaag gcaatgcagg gcctgctgaa agacggtaat     1560

ccgatcccat ctgcaatcgc tgctaactct ggcatttact aataagcgga cgcgctgcca     1620

ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt     1680

tgctgaaagg aggaactata tccggcatgc accattcctt gcggcggcgg tgctcaacgg     1740

cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc     1800

gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat     1860

cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc     1920

gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc     1980

gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac     2040

caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta     2100

cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc     2160

ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga     2220

ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcattgg     2280

accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg     2340

gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag     2400

ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca     2460

agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag     2520

aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt     2580

gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg     2640

cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac     2700

tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt     2760

tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc     2820

gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat     2880

tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca     2940

caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct     3000

cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg     3060

accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg     3120

cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt     3180

cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa     3240

aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg     3300

agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg     3360

acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga     3420

ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat     3480

accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc     3540

tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg     3600

ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg     3660

ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac     3720

gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg     3780

gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct     3840

ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg     3900

tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct     3960

gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac     4020

tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt     4080

tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc     4140

tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca     4200

ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat     4260

ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac     4320

gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt     4380

aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc     4440

aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg     4500

cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg     4560

ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc     4620

cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta     4680

ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg     4740

ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct     4800

ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta     4860

gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg     4920

ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga     4980

ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt     5040

gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca     5100

ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt     5160

cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt     5220

ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga     5280

aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt     5340

gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc     5400

gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa     5460

cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaa                      5504


<210>  28
<211>  68
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminus truncated bacteriophage MS2 coat protein sequence

<400>  28

Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp Asn Gly Gly Thr 
1               5                   10                  15      


Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn Gly Val Ala Glu 
            20                  25                  30          


Trp Ile Ser Ser Asn Ser Arg Ser Gln Gly Ala Pro Phe Val Ser Leu 
        35                  40                  45              


Ala Arg Arg Ile Ala Asn Thr Pro Ser Lys Leu Lys Tyr Arg Lys Ser 
    50                  55                  60                  


Gln Arg Arg Pro 
65              


<210>  29
<211>  8339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10429; C. glutamicum rnc deletion 
       plasmid


<220>
<221>  gene
<222>  (346)..(1140)
<223>  Kanamycin resistance gene

<220>
<221>  promoter
<222>  (1186)..(1631)
<223>  sacB promoter

<220>
<221>  gene
<222>  (1632)..(3053)
<223>  sacB gene

<220>
<221>  misc_feature
<222>  (5504)..(5510)
<223>  restriction endonuclease BamHI recognition site

<220>
<221>  misc_feature
<222>  (5511)..(8136)
<223>  PCR fragment comprising 1.5 kb of chromosomal sequencedownstream 
       of rnc gene (5511-7012) and 1.2 kb of chromosomal sequence 
       upstream of rnc gene (7013-8136).

<220>
<221>  misc_feature
<222>  (8136)..(8141)
<223>  restrcition endonuclease SalI recognition site

<400>  29
tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt       60

ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa      120

aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac      180

tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag      240

gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc      300

aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat      360

ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca      420

caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg      480

gttctttttg tcaagaccga cctgtccggt gccctgaatg aactccaaga cgaggcagcg      540

cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact      600

gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct      660

caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg      720

cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt      780

actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc      840

gcgccagccg aactgttcgc caggctcaag gcgcggatgc ccgacggcga ggatctcgtc      900

gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga      960

ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc     1020

cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt     1080

atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga     1140

gcgggactct ggggttcgct agaggatcga tcctttttaa cccatcacat atacctgccg     1200

ttcactatta tttagtgaaa tgagatatta tgatattttc tgaattgtga ttaaaaaggc     1260

aactttatgc ccatgcaaca gaaactataa aaaatacaga gaatgaaaag aaacagatag     1320

attttttagt tctttaggcc cgtagtctgc aaatcctttt atgattttct atcaaacaaa     1380

agaggaaaat agaccagttg caatccaaac gagagtctaa tagaatgagg tcgaaaagta     1440

aatcgcgcgg gtttgttact gataaagcag gcaagaccta aaatgtgtaa agggcaaagt     1500

gtatactttg gcgtcacccc ttacatattt taggtctttt tttattgtgc gtaactaact     1560

tgccatcttc aaacaggagg gctggaagaa gcagaccgct aacacagtac ataaaaaagg     1620

agacatgaac gatgaacatc aaaaagtttg caaaacaagc aacagtatta acctttacta     1680

ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac caaaagccat     1740

ataaggaaac atacggcatt tcccatatta cacgccatga tatgctgcaa atccctgaac     1800

agcaaaaaaa tgaaaaatat caagtttctg aatttgattc gtccacaatt aaaaatatct     1860

cttctgcaaa aggcctggac gtttgggaca gctggccatt acaaaacgct gacggcactg     1920

tcgcaaacta tcacggctac cacatcgtct ttgcattagc cggagatcct aaaaatgcgg     1980

atgacacatc gatttacatg ttctatcaaa aagtcggcga aacttctatt gacagctgga     2040

aaaacgctgg ccgcgtcttt aaagacagcg acaaattcga tgcaaatgat tctatcctaa     2100

aagaccaaac acaagaatgg tcaggttcag ccacatttac atctgacgga aaaatccgtt     2160

tattctacac tgatttctcc ggtaaacatt acggcaaaca aacactgaca actgcacaag     2220

ttaacgtatc agcatcagac agctctttga acatcaacgg tgtagaggat tataaatcaa     2280

tctttgacgg tgacggaaaa acgtatcaaa atgtacagca gttcatcgat gaaggcaact     2340

acagctcagg cgacaaccat acgctgagag atcctcacta cgtagaagat aaaggccaca     2400

aatacttagt atttgaagca aacactggaa ctgaagatgg ctaccaaggc gaagaatctt     2460

tatttaacaa agcatactat ggcaaaagca catcattctt ccgtcaagaa agtcaaaaac     2520

ttctgcaaag cgataaaaaa cgcacggctg agttagcaaa cggcgctctc ggtatgattg     2580

agctaaacga tgattacaca ctgaaaaaag tgatgaaacc gctgattgca tctaacacag     2640

taacagatga aattgaacgc gcgaacgtct ttaaaatgaa cggcaaatgg tacctgttca     2700

ctgactcccg cggatcaaaa atgacgattg acggcattac gtctaacgat atttacatgc     2760

ttggttatgt ttctaattct ttaactggcc catacaagcc gctgaacaaa actggccttg     2820

tgttaaaaat ggatcttgat cctaacgatg taacctttac ttactcacac ttcgctgtac     2880

ctcaagcgaa aggaaacaat gtcgtgatta caagctatat gacaaacaga ggattctacg     2940

cagacaaaca atcaacgttt gcgccgagct tcctgctgaa catcaaaggc aagaaaacat     3000

ctgttgtcaa agacagcatc cttgaacaag gacaattaac agttaacaaa taaaaacgca     3060

aaagaaaatg ccgatgggta ccgagcgaaa tgaccgacca agcgacgccc aacctgccat     3120

cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc     3180

gggacgccct cgcggacgtg ctcatagtcc acgacgcccg tgattttgta gccctggccg     3240

acggccagca ggtaggccga caggctcatg ccggccgccg ccgccttttc ctcaatcgct     3300

cttcgttcgt ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttggcttg     3360

gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc     3420

agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac     3480

ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa     3540

ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat     3600

accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa gcgctgcttc     3660

cctgctgttt tgtggaatat ctaccgactg gaaacaggca aatgcaggaa attactgaac     3720

tgaggggaca ggcgagagac gatgccaaag agctcctgaa aatctcgata actcaaaaaa     3780

tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc     3840

aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg     3900

atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc     3960

gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg     4020

cttctgtttc tatcagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat     4080

cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc     4140

caaaagttgg cccagggctt cccggtatca acagggacac caggatttat ttattctgcg     4200

aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac     4260

ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag     4320

ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaaaagg     4380

atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg     4440

ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt     4500

ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg     4560

ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata     4620

ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca     4680

ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag     4740

tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc     4800

tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga     4860

tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg     4920

tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac     4980

gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg     5040

tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg     5100

ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct     5160

gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc     5220

gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc     5280

cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg     5340

ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta     5400

cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca     5460

ggaaacagct atgacatgat tacgaattcg agctcggtac ccggggatcc caaatccaga     5520

aaagctcata ctgctcccct aatcgatggc ttccccagca agggctttgg aggcgaggcg     5580

ttcttcctca ctgacttccc acacctcgtg agcggcgtgc agtgcgagca gaatgatgaa     5640

acaacctagg atcaaatctg gccatccaga cgtcgtccat gcggtaatta aggccatcat     5700

gatgatggca atgttgatca ggacgtcatt tcgggcggat aggaaggcag cttggccaag     5760

cgagccacca tgttgtcgca ctcgagaaat aatgatggca ctcgcgccgt tgatcacgac     5820

ggcgcccaga gaagcgacga tgatcggaaa cacttcgggc gcttgcggtg cggaaaaccg     5880

ttgaatcgct gcccacgcag caaaagcagc aggtgcaaga atcacaatcg ccataagttt     5940

gcccatcact gcgcgcctcg ccaacggcca tcctagggca atgaaaatga gcaggttgat     6000

ggaggtgtct tcaagaaaat cgacactgtc agccagtaga gaaacggagc ctgcgcttaa     6060

tgcaataaag aattctacaa agaaataagc gaagttaagc agcgcgacgg tgagcacagc     6120

tttgcgcact ttggttgcat caaaagcttc gctcatcagc tagcgccgct tctggcagtt     6180

tgggcagtag tgggagccgc ggttcatgaa actctcccgg atgattaatg ttccgcagcg     6240

tccgcacggc tccccggttt gcgcataagc attcaatgac agcgcaaagt agccggagtt     6300

gccattgacg ttgacataga gcgcgtcgaa agaggtgcca ccttgagcaa gtgctttggt     6360

catcacgtct ttgccagctt gaagaagttc ttccaagcga gctagggaca gtcgatcggc     6420

acgttgcaat gggtgaattt ttgcttgcca gagcatttca tcggcataga tatttccgat     6480

gccggagacg atctcttggt taagcaggag gcgtttgatc tccgatttcc gagatttcaa     6540

attccgcgca atcgcagaga aatcagcaga ctcatccaat acatctgtgg caatgtgaga     6600

gacgcgttcg ggtactccat caactaggtc gccgagccac caataaccga aggtgcgttg     6660

atcgacaaac cacacttcat cgccattatc tagctcgact ttggctcgaa ggtgtggact     6720

aattggtgca tctggttctt tgatgagcat ttgtccactc atcccaaggt gaaccagtag     6780

ccctaaatcg ggacgggttt cgccggaggg tgcgtcgata agctcaagcc agaggaattt     6840

gccgcgtcgc ttggcagcgc tgaccctaag ccctgcgatg ttggcctcga tttcggggcc     6900

accgccgagt tgattgcggg ctgcgcgcgg gtgaagcact gtggcggaca cgatggtgtg     6960

gccgaccata tgatcttcta aaccgcggcg caccacctca acttcaggca gtaggaactt     7020

ctccaaacca gcccagcgcg gatcgacctt ttcctcgtct tctacttctt cagagacacc     7080

gtcaggagct ggcgtttcat catcctggca tgcgccgtac ccaagttctt cgcagacagg     7140

gttaaacggc aaggtcagac cagcttcatc aatgacagac tgaagcagat caatctggtc     7200

ttggttaacc attggcagct catcttcgtc atctgctgca tcttcaccag taacaaagtc     7260

tggatcggca gcaaaaacct cagagacgtg cagcgtcttg gttggggtga gttcgcggag     7320

gcagcgggag cactgtccca gaagctgcgc ttcgatatct gcttcgacgg ccaggcctcc     7380

accgagtgga atgatctggg cttctacgat aacttttccg ccctcgggga tcgcgatcat     7440

ttccggacca atgcgggtcg ggcttggacc tgattgggtg aggtgttccg gaagggcact     7500

tccacgaagg agtgcggcga catcaaaaat aaatggagat ttcatgacca ggaagatcct     7560

actcgtacct catcgtgact acatacatca gatgaggatc gctagacgga aaggtgaaaa     7620

agcctaaatg gtttcttgga aactagtagt ctcgatcgtc gcgttcgtag cctcgttcat     7680

aatcacgctc gtagtcccgc tcatattcac cacgcgctgc cggttgctca tcgcgcagct     7740

cgcggccagt agctccagct cctcggcgta gtgcggaacg atcagcagtg acagaacgca     7800

acgtggtgga cagcgaggtt tcaaactctg ccaacttggt atccacgtag tcatcgcatt     7860

cattgcgcag cttgttggag tcagcgtgcg ctgcatccac aatgcggtgt gcttcttccg     7920

tggagcgacg caccacttct gcctcgctga ccaggcgatc ctgctcggcc tggccttcag     7980

caaccgcgcg gcgatacgca tcattgccgg agttgaccag gcgctcagat tcagcctggg     8040

cgcgctcggt gacgctgttt gcttctcgac gagcgtcact cacgatgcga tccgctgtgt     8100

cattagcctt agcaacgaca gaatgcgcat gcttgggtcg acctgcaggc atgcaagctt     8160

ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa     8220

tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga     8280

tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgataagcta gcttcacgc      8339


<210>  30
<211>  3343
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic DNA


<220>
<221>  misc_feature
<222>  (1)..(301)
<223>  pCG1 origin of replication

<220>
<221>  gene
<222>  (2269)..(3063)
<223>  kanamycin resistance gene

<400>  30
ccatggtcgt cacagagctg gaagcggcag cgagaattat ccgcgatcgt ggcgcggtgc       60

ccgcaggcat gacaaacatc gtaaatgccg cgtttcgtgt ggccgtggcc gcccaggacg      120

tgtcagcgcc gccaccacct gcaccgaatc ggcagcagcg tcgcgcgtcg aaaaagcgca      180

caggcggcaa gaagcgataa gctgcacgaa tacctgaaaa atgttgaacg ccccgtgagc      240

ggtaactcac agggcgtcgg ctaaccccca gtccaaacct gggagaaagc gctcaaaaat      300

gactctagcg gattcacgag acattgacac accggcctgg aaattttccg ctgatctgtt      360

cgacacccat cccgagctcg cgctgcgatc acgtggctgg acgagcgaag accgccgcga      420

attcctcgct cacctgggca gagaaaattt ccagggcagc aagacccgcg acttcgccag      480

cgcttggatc aaagacccgg acacgggaga aacacagccg aagttatacc gagttggttc      540

aaaatcgctt gcccggtgcc agtatgttgc tctgacgcac gcgcagcacg cagccgtgct      600

tgtcctggac attgatgtgc cgagccacca ggccggcggg aaaatcgagc acgtaaaccc      660

cgaggtctac gcgattttgg agcgctgggc acgcctggaa aaagcgccag cttggatcgg      720

cgtgaatcca ctgagcggga aatgccagct catctggctc attgatccgg tgtatgccgc      780

agcaggcatg agcagcccga atatgcgcct gctggctgca acgaccgagg aaatgacccg      840

cgttttcggc gctgaccagg ctttttcaca taggctgagc cggtggccac tgcacgtctc      900

cgacgatccc accgcgtacc gctggcatgc ccagcacaat cgcgtggatc gcctagctga      960

tcttatggag gttgctcgca tgatctcagg cacagaaaaa cctaaaaaac gctatgagca     1020

ggagttttct agcggacggg cacgtatcga agcggcaaga aaagccactg cggaagcaaa     1080

agcacttgcc acgcttgaag caagcctgcc gagcgccgct gaagcgtctg gagagctgat     1140

cgacggcgtc cgtgtcctct ggactgctcc agggcgtgcc gcccgtgatg agacggcttt     1200

tcgccacgct ttgactgtgg gataccagtt aaaagcggct ggtgagcgcc taaaagacac     1260

caagatcatc gacgcctacg agcgtgccta caccgtcgct caggcggtcg gagcagacgg     1320

ccgtgagcct gatctgccgc cgatgcgtga ccgccagacg atggcgcgac gtgtgcgcgg     1380

ctacgtcgct aaaggccagc cagtcgtccc tgctcgtcag acagagacgc agagcagccg     1440

agggcgaaaa gctctggcca ctatgggaag acgtggcggt aaaaaggccg cagaacgctg     1500

gaaagaccca aacagtgagt acgcccgagc acagcgagaa aaactagcta agtccagtca     1560

acgacaagct aggaaagcta aaggaaatcg cttgaccatt gcaggttggt ttatgactgt     1620

tgagggagag actggctcgt ggcgacaatc aatgaagcta tgtctgaatt tagcgtgtca     1680

cgtcagaccg tgaatagagc acttaagtct gcgggcattg aacttccacg aggacgccgt     1740

aaagcttccc agtaaatgtg ccatctcgta ggcagaaaac ggttcccccc gtaggggtct     1800

ctctcttggc ctcctttcta ggtcgggctg attgctcttg aagctctcta ggggggctca     1860

caccataggc agataacggt tccccaccgg ctcacctcgt aagcgcacaa ggactgctcc     1920

caatgccgca agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc     1980

agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg     2040

gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta     2100

gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt     2160

aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg     2220

cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa     2280

gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg     2340

gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc     2400

ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactcca agacgaggca     2460

gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc     2520

actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca     2580

tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat     2640

acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca     2700

cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg     2760

ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgga tgcccgacgg cgaggatctc     2820

gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct     2880

ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct     2940

acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac     3000

ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc     3060

tgagcgggac tctggggttc gctagaggat cgatcctttt taacccatca catatacctg     3120

ccgttcacta ttatttagtg aaatgagata ttatgatatt ttctgaattg tgattaaaaa     3180

ggcaacttta tgcccatgca acagaaacta taaaaaatac agagaatgaa aagaaacaga     3240

tagatttttt agttctttag gcccgtagtc tgcaaatcct tttatgattt tctatcaaac     3300

aaaagaggaa aatagaccag ttgcaatcca aacgagagtc taa                       3343


<210>  31
<211>  8710
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10430; beta actin hairpin + coat protein


<220>
<221>  promoter
<222>  (38)..(56)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (60)..(67)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (68)..(362)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (362)..(369)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (527)..(821)
<223>  beta actin antisense strand gene fragment

<220>
<221>  terminator
<222>  (887)..(934)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (985)..(1003)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1076)..(1465)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (1498)..(1545)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  misc_feature
<222>  (1981)..(5324)
<223>  synthetic DNA (SEQ ID NO: 30)

<400>  31
ttcttagctc cggcaagcaa ttaagaactt ccgaaattaa tacgactcac tatagggagg       60

cgatcgcgca cgaggttttt ctgtctagtg agcagtgtcc aacctcaaaa gacaacatgt      120

gtgacgacga tgtagcggct cttgtcgtag acaatggatc cggtatgtgc aaagccggtt      180

tcgcaggaga tgacgcaccc cgtgccgtct tcccctcgat cgtcggtcgc ccaaggcatc      240

aaggagtcat ggtcggtatg ggacaaaagg actcatacgt aggagatgaa gcccaaagca      300

aaagaggtat cctcaccctg aaatacccca tcgaacacgg tatcatcacc aactgggatg      360

agtttaaacc ctctagctgc tttacaaagt actggttccc tttccagcgg gatgctttat      420

ctaaacgcaa tgagagaggt attcctcagg ccacatcgct tcctagttcc gctgggatcc      480

atcgttggcg gccgaagccg ccattccata gtgagttctg gcgcgcctca tcccagttgg      540

tgatgatacc gtgttcgatg gggtatttca gggtgaggat acctcttttg ctttgggctt      600

catctcctac gtatgagtcc ttttgtccca taccgaccat gactccttga tgccttgggc      660

gaccgacgat cgaggggaag acggcacggg gtgcgtcatc tcctgcgaaa ccggctttgc      720

acataccgga tccattgtct acgacaagag ccgctacatc gtcgtcacac atgttgtctt      780

ttgaggttgg acactgctca ctagacagaa aaacctcgtg ccggaccgaa tacccggtct      840

gaacgaggtt aattaaggta cccaagaagt acttagaggc ggccgcctag cataacccct      900

tggggcctct aaacgggtct tgaggggttt tttgagaaac ggccgaatac acctgttcgg      960

atccagatct cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc     1020

cctctagatc acaagtttgt acaaaaaagc aggctaagaa ggagatatac atatggcgtc     1080

taactttacc caattcgttc tggttgataa cggcggtacg ggtgacgtta ccgtagctcc     1140

gtccaacttc gccaacggtg ttgcggaatg gattagctct aacagccgct ctcaggccta     1200

caaagtcacg tgctccgttc gtcagtctag cgcgcagaat cgcaaataca ccatcaaagt     1260

tgaagtaccg aaagtcgcaa cgcagaccgt aggcggcgta gaactcccag ttgcggcctg     1320

gcgctcttac ctcaacatgg aactgactat tccgattttt gcgacgaact ccgactgcga     1380

actgattgtt aaggcaatgc agggcctgct gaaagacggt aatccgatcc catctgcaat     1440

cgctgctaac tctggcattt actaataagc ggacgcgctg ccaccgctga gcaataacta     1500

gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact     1560

atatccggca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg     1620

gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct     1680

tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga     1740

ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg     1800

gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa     1860

tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga     1920

agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg     1980

ccatggtcgt cacagagctg gaagcggcag cgagaattat ccgcgatcgt ggcgcggtgc     2040

ccgcaggcat gacaaacatc gtaaatgccg cgtttcgtgt ggccgtggcc gcccaggacg     2100

tgtcagcgcc gccaccacct gcaccgaatc ggcagcagcg tcgcgcgtcg aaaaagcgca     2160

caggcggcaa gaagcgataa gctgcacgaa tacctgaaaa atgttgaacg ccccgtgagc     2220

ggtaactcac agggcgtcgg ctaaccccca gtccaaacct gggagaaagc gctcaaaaat     2280

gactctagcg gattcacgag acattgacac accggcctgg aaattttccg ctgatctgtt     2340

cgacacccat cccgagctcg cgctgcgatc acgtggctgg acgagcgaag accgccgcga     2400

attcctcgct cacctgggca gagaaaattt ccagggcagc aagacccgcg acttcgccag     2460

cgcttggatc aaagacccgg acacgggaga aacacagccg aagttatacc gagttggttc     2520

aaaatcgctt gcccggtgcc agtatgttgc tctgacgcac gcgcagcacg cagccgtgct     2580

tgtcctggac attgatgtgc cgagccacca ggccggcggg aaaatcgagc acgtaaaccc     2640

cgaggtctac gcgattttgg agcgctgggc acgcctggaa aaagcgccag cttggatcgg     2700

cgtgaatcca ctgagcggga aatgccagct catctggctc attgatccgg tgtatgccgc     2760

agcaggcatg agcagcccga atatgcgcct gctggctgca acgaccgagg aaatgacccg     2820

cgttttcggc gctgaccagg ctttttcaca taggctgagc cggtggccac tgcacgtctc     2880

cgacgatccc accgcgtacc gctggcatgc ccagcacaat cgcgtggatc gcctagctga     2940

tcttatggag gttgctcgca tgatctcagg cacagaaaaa cctaaaaaac gctatgagca     3000

ggagttttct agcggacggg cacgtatcga agcggcaaga aaagccactg cggaagcaaa     3060

agcacttgcc acgcttgaag caagcctgcc gagcgccgct gaagcgtctg gagagctgat     3120

cgacggcgtc cgtgtcctct ggactgctcc agggcgtgcc gcccgtgatg agacggcttt     3180

tcgccacgct ttgactgtgg gataccagtt aaaagcggct ggtgagcgcc taaaagacac     3240

caagatcatc gacgcctacg agcgtgccta caccgtcgct caggcggtcg gagcagacgg     3300

ccgtgagcct gatctgccgc cgatgcgtga ccgccagacg atggcgcgac gtgtgcgcgg     3360

ctacgtcgct aaaggccagc cagtcgtccc tgctcgtcag acagagacgc agagcagccg     3420

agggcgaaaa gctctggcca ctatgggaag acgtggcggt aaaaaggccg cagaacgctg     3480

gaaagaccca aacagtgagt acgcccgagc acagcgagaa aaactagcta agtccagtca     3540

acgacaagct aggaaagcta aaggaaatcg cttgaccatt gcaggttggt ttatgactgt     3600

tgagggagag actggctcgt ggcgacaatc aatgaagcta tgtctgaatt tagcgtgtca     3660

cgtcagaccg tgaatagagc acttaagtct gcgggcattg aacttccacg aggacgccgt     3720

aaagcttccc agtaaatgtg ccatctcgta ggcagaaaac ggttcccccc gtaggggtct     3780

ctctcttggc ctcctttcta ggtcgggctg attgctcttg aagctctcta ggggggctca     3840

caccataggc agataacggt tccccaccgg ctcacctcgt aagcgcacaa ggactgctcc     3900

caatgccgca agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc     3960

agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg     4020

gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta     4080

gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt     4140

aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg     4200

cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa     4260

gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg     4320

gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc     4380

ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactcca agacgaggca     4440

gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc     4500

actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca     4560

tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat     4620

acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca     4680

cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg     4740

ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgga tgcccgacgg cgaggatctc     4800

gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct     4860

ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct     4920

acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac     4980

ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc     5040

tgagcgggac tctggggttc gctagaggat cgatcctttt taacccatca catatacctg     5100

ccgttcacta ttatttagtg aaatgagata ttatgatatt ttctgaattg tgattaaaaa     5160

ggcaacttta tgcccatgca acagaaacta taaaaaatac agagaatgaa aagaaacaga     5220

tagatttttt agttctttag gcccgtagtc tgcaaatcct tttatgattt tctatcaaac     5280

aaaagaggaa aatagaccag ttgcaatcca aacgagagtc taacgacgcg aggctggatg     5340

gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc     5400

atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct     5460

cttaccagcc taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg     5520

gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc     5580

cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc     5640

acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg     5700

tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc     5760

gcacgcggcg catctcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc     5820

tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac     5880

cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa     5940

catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct     6000

gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta     6060

catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca     6120

tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag     6180

taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa     6240

atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc     6300

gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg     6360

aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc     6420

tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     6480

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg     6540

ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg     6600

gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat     6660

accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac     6720

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     6780

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     6840

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     6900

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     6960

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     7020

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     7080

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     7140

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     7200

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     7260

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag     7320

aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     7380

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     7440

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     7500

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag     7560

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata     7620

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat     7680

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg     7740

ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc     7800

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc     7860

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc     7920

gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc     7980

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc     8040

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa     8100

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat     8160

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata     8220

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca     8280

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag     8340

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc     8400

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc     8460

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata     8520

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta     8580

gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta     8640

agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg     8700

tcttcaagaa                                                            8710


<210>  32
<211>  8107
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10431; beta actin stem loop - coat 
       protein


<220>
<221>  promoter
<222>  (38)..(56)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (60)..(67)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (68)..(362)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (362)..(369)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (527)..(821)
<223>  beta actin antisense strand gene fragment

<220>
<221>  terminator
<222>  (887)..(934)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  misc_feature
<222>  (1378)..(4721)
<223>  synthetic DNA (SEQ ID NO: 30)

<400>  32
ttcttagctc cggcaagcaa ttaagaactt ccgaaattaa tacgactcac tatagggagg       60

cgatcgcgca cgaggttttt ctgtctagtg agcagtgtcc aacctcaaaa gacaacatgt      120

gtgacgacga tgtagcggct cttgtcgtag acaatggatc cggtatgtgc aaagccggtt      180

tcgcaggaga tgacgcaccc cgtgccgtct tcccctcgat cgtcggtcgc ccaaggcatc      240

aaggagtcat ggtcggtatg ggacaaaagg actcatacgt aggagatgaa gcccaaagca      300

aaagaggtat cctcaccctg aaatacccca tcgaacacgg tatcatcacc aactgggatg      360

agtttaaacc ctctagctgc tttacaaagt actggttccc tttccagcgg gatgctttat      420

ctaaacgcaa tgagagaggt attcctcagg ccacatcgct tcctagttcc gctgggatcc      480

atcgttggcg gccgaagccg ccattccata gtgagttctg gcgcgcctca tcccagttgg      540

tgatgatacc gtgttcgatg gggtatttca gggtgaggat acctcttttg ctttgggctt      600

catctcctac gtatgagtcc ttttgtccca taccgaccat gactccttga tgccttgggc      660

gaccgacgat cgaggggaag acggcacggg gtgcgtcatc tcctgcgaaa ccggctttgc      720

acataccgga tccattgtct acgacaagag ccgctacatc gtcgtcacac atgttgtctt      780

ttgaggttgg acactgctca ctagacagaa aaacctcgtg ccggaccgaa tacccggtct      840

gaacgaggtt aattaaggta cccaagaagt acttagaggc ggccgcctag cataacccct      900

tggggcctct aaacgggtct tgaggggttt tttgagaaac ggccgaatac acctgttcgg      960

atccagatcc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct     1020

gcttcctaat gcaggagtcg cataagggag agcgtcgacc gatgcccttg agagccttca     1080

acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg     1140

tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg     1200

aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct     1260

tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc     1320

aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcca     1380

tggtcgtcac agagctggaa gcggcagcga gaattatccg cgatcgtggc gcggtgcccg     1440

caggcatgac aaacatcgta aatgccgcgt ttcgtgtggc cgtggccgcc caggacgtgt     1500

cagcgccgcc accacctgca ccgaatcggc agcagcgtcg cgcgtcgaaa aagcgcacag     1560

gcggcaagaa gcgataagct gcacgaatac ctgaaaaatg ttgaacgccc cgtgagcggt     1620

aactcacagg gcgtcggcta acccccagtc caaacctggg agaaagcgct caaaaatgac     1680

tctagcggat tcacgagaca ttgacacacc ggcctggaaa ttttccgctg atctgttcga     1740

cacccatccc gagctcgcgc tgcgatcacg tggctggacg agcgaagacc gccgcgaatt     1800

cctcgctcac ctgggcagag aaaatttcca gggcagcaag acccgcgact tcgccagcgc     1860

ttggatcaaa gacccggaca cgggagaaac acagccgaag ttataccgag ttggttcaaa     1920

atcgcttgcc cggtgccagt atgttgctct gacgcacgcg cagcacgcag ccgtgcttgt     1980

cctggacatt gatgtgccga gccaccaggc cggcgggaaa atcgagcacg taaaccccga     2040

ggtctacgcg attttggagc gctgggcacg cctggaaaaa gcgccagctt ggatcggcgt     2100

gaatccactg agcgggaaat gccagctcat ctggctcatt gatccggtgt atgccgcagc     2160

aggcatgagc agcccgaata tgcgcctgct ggctgcaacg accgaggaaa tgacccgcgt     2220

tttcggcgct gaccaggctt tttcacatag gctgagccgg tggccactgc acgtctccga     2280

cgatcccacc gcgtaccgct ggcatgccca gcacaatcgc gtggatcgcc tagctgatct     2340

tatggaggtt gctcgcatga tctcaggcac agaaaaacct aaaaaacgct atgagcagga     2400

gttttctagc ggacgggcac gtatcgaagc ggcaagaaaa gccactgcgg aagcaaaagc     2460

acttgccacg cttgaagcaa gcctgccgag cgccgctgaa gcgtctggag agctgatcga     2520

cggcgtccgt gtcctctgga ctgctccagg gcgtgccgcc cgtgatgaga cggcttttcg     2580

ccacgctttg actgtgggat accagttaaa agcggctggt gagcgcctaa aagacaccaa     2640

gatcatcgac gcctacgagc gtgcctacac cgtcgctcag gcggtcggag cagacggccg     2700

tgagcctgat ctgccgccga tgcgtgaccg ccagacgatg gcgcgacgtg tgcgcggcta     2760

cgtcgctaaa ggccagccag tcgtccctgc tcgtcagaca gagacgcaga gcagccgagg     2820

gcgaaaagct ctggccacta tgggaagacg tggcggtaaa aaggccgcag aacgctggaa     2880

agacccaaac agtgagtacg cccgagcaca gcgagaaaaa ctagctaagt ccagtcaacg     2940

acaagctagg aaagctaaag gaaatcgctt gaccattgca ggttggttta tgactgttga     3000

gggagagact ggctcgtggc gacaatcaat gaagctatgt ctgaatttag cgtgtcacgt     3060

cagaccgtga atagagcact taagtctgcg ggcattgaac ttccacgagg acgccgtaaa     3120

gcttcccagt aaatgtgcca tctcgtaggc agaaaacggt tccccccgta ggggtctctc     3180

tcttggcctc ctttctaggt cgggctgatt gctcttgaag ctctctaggg gggctcacac     3240

cataggcaga taacggttcc ccaccggctc acctcgtaag cgcacaagga ctgctcccaa     3300

tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt     3360

ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa     3420

aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac     3480

tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag     3540

gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc     3600

aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat     3660

ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca     3720

caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg     3780

gttctttttg tcaagaccga cctgtccggt gccctgaatg aactccaaga cgaggcagcg     3840

cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact     3900

gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct     3960

caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg     4020

cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt     4080

actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc     4140

gcgccagccg aactgttcgc caggctcaag gcgcggatgc ccgacggcga ggatctcgtc     4200

gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga     4260

ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc     4320

cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt     4380

atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga     4440

gcgggactct ggggttcgct agaggatcga tcctttttaa cccatcacat atacctgccg     4500

ttcactatta tttagtgaaa tgagatatta tgatattttc tgaattgtga ttaaaaaggc     4560

aactttatgc ccatgcaaca gaaactataa aaaatacaga gaatgaaaag aaacagatag     4620

attttttagt tctttaggcc cgtagtctgc aaatcctttt atgattttct atcaaacaaa     4680

agaggaaaat agaccagttg caatccaaac gagagtctaa cgacgcgagg ctggatggcc     4740

ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg     4800

ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt     4860

accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg     4920

agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc     4980

gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc     5040

tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga     5100

atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc agcagccgca     5160

cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt     5220

cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga     5280

tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat     5340

gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca     5400

ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat     5460

ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc     5520

ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa     5580

cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc     5640

ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct     5700

ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac     5760

aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg     5820

cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag     5880

cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg     5940

gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct     6000

taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc     6060

gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga     6120

ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat     6180

acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca     6240

aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc     6300

tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata     6360

aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc     6420

gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc     6480

acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga     6540

accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc     6600

ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag     6660

gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag     6720

gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag     6780

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca     6840

gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga     6900

cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat     6960

cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga     7020

gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg     7080

tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga     7140

gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc     7200

agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac     7260

tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc     7320

agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc     7380

gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc     7440

catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt     7500

ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc     7560

atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg     7620

tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag     7680

cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat     7740

cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc     7800

atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa     7860

aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta     7920

ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa     7980

aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga     8040

aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct     8100

tcaagaa                                                               8107


<210>  33
<211>  9000
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10432; beta actin hairpin + coat protein


<220>
<221>  promoter
<222>  (10)..(28)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (32)..(39)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (41)..(334)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (334)..(341)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (498)..(793)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (868)..(886)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (902)..(949)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1275)..(1293)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1366)..(1755)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (1788)..(1835)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  misc_feature
<222>  (2272)..(5614)
<223>  synthetic DNA (SEQ ID NO: 30)

<400>  33
ttccgaaatt aatacgactc actataggga ggcgatcgcg cacgaggttt ttctgtctag       60

tgagcagtgt ccaacctcaa aagacaacat gtgtgacgac gatgtagcgg ctcttgtcgt      120

agacaatgga tccggtatgt gcaaagccgg tttcgcagga gatgacgcac cccgtgccgt      180

cttcccctcg atcgtcggtc gcccaaggca tcaaggagtc atggtcggta tgggacaaaa      240

ggactcatac gtaggagatg aagcccaaag caaaagaggt atcctcaccc tgaaataccc      300

catcgaacac ggtatcatca ccaactggga tgagtttaaa ccctctagct gctttacaaa      360

gtactggttc cctttccagc gggatgcttt atctaaacgc aatgagagag gtattcctca      420

ggccacatcg cttcctagtt ccgctgggat ccatcgttgg cggccgaagc cgccattcca      480

tagtgagttc tggcgcgcct catcccagtt ggtgatgata ccgtgttcga tggggtattt      540

cagggtgagg atacctcttt tgctttgggc ttcatctcct acgtatgagt ccttttgtcc      600

cataccgacc atgactcctt gatgccttgg gcgaccgacg atcgagggga agacggcacg      660

gggtgcgtca tctcctgcga aaccggcttt gcacataccg gatccattgt ctacgacaag      720

agccgctaca tcgtcgtcac acatgttgtc ttttgaggtt ggacactgct cactagacag      780

aaaaacctcg tgccggaccg aatacccggt ctgaacgagg gcggccgcgg tacccaagaa      840

gtacttagag ttaattaagg agttcaaaca tgaggatcac ccatgtcgaa gctcccacac      900

cctagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg      960

aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg     1020

ctccaagtag cgaagcgagc aggactgggc ggcgggcatg catcgtccat tccgacagca     1080

tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac     1140

ccgttctcgg agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac     1200

ttggagccac tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg atccagatct     1260

cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagatc     1320

acaagtttgt acaaaaaagc aggctaagaa ggagatatac atatggcgtc taactttacc     1380

caattcgttc tggttgataa cggcggtacg ggtgacgtta ccgtagctcc gtccaacttc     1440

gccaacggtg ttgcggaatg gattagctct aacagccgct ctcaggccta caaagtcacg     1500

tgctccgttc gtcagtctag cgcgcagaat cgcaaataca ccatcaaagt tgaagtaccg     1560

aaagtcgcaa cgcagaccgt aggcggcgta gaactcccag ttgcggcctg gcgctcttac     1620

ctcaacatgg aactgactat tccgattttt gcgacgaact ccgactgcga actgattgtt     1680

aaggcaatgc agggcctgct gaaagacggt aatccgatcc catctgcaat cgctgctaac     1740

tctggcattt actaataagc ggacgcgctg ccaccgctga gcaataacta gcataacccc     1800

ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggca     1860

tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct     1920

aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt     1980

cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt     2040

tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg     2100

ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc     2160

cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat     2220

tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg ccatggtcgt     2280

cacagagctg gaagcggcag cgagaattat ccgcgatcgt ggcgcggtgc ccgcaggcat     2340

gacaaacatc gtaaatgccg cgtttcgtgt ggccgtggcc gcccaggacg tgtcagcgcc     2400

gccaccacct gcaccgaatc ggcagcagcg tcgcgcgtcg aaaaagcgca caggcggcaa     2460

gaagcgataa gctgcacgaa tacctgaaaa atgttgaacg ccccgtgagc ggtaactcac     2520

agggcgtcgg ctaaccccca gtccaaacct gggagaaagc gctcaaaaat gactctagcg     2580

gattcacgag acattgacac accggcctgg aaattttccg ctgatctgtt cgacacccat     2640

cccgagctcg cgctgcgatc acgtggctgg acgagcgaag accgccgcga attcctcgct     2700

cacctgggca gagaaaattt ccagggcagc aagacccgcg acttcgccag cgcttggatc     2760

aaagacccgg acacgggaga aacacagccg aagttatacc gagttggttc aaaatcgctt     2820

gcccggtgcc agtatgttgc tctgacgcac gcgcagcacg cagccgtgct tgtcctggac     2880

attgatgtgc cgagccacca ggccggcggg aaaatcgagc acgtaaaccc cgaggtctac     2940

gcgattttgg agcgctgggc acgcctggaa aaagcgccag cttggatcgg cgtgaatcca     3000

ctgagcggga aatgccagct catctggctc attgatccgg tgtatgccgc agcaggcatg     3060

agcagcccga atatgcgcct gctggctgca acgaccgagg aaatgacccg cgttttcggc     3120

gctgaccagg ctttttcaca taggctgagc cggtggccac tgcacgtctc cgacgatccc     3180

accgcgtacc gctggcatgc ccagcacaat cgcgtggatc gcctagctga tcttatggag     3240

gttgctcgca tgatctcagg cacagaaaaa cctaaaaaac gctatgagca ggagttttct     3300

agcggacggg cacgtatcga agcggcaaga aaagccactg cggaagcaaa agcacttgcc     3360

acgcttgaag caagcctgcc gagcgccgct gaagcgtctg gagagctgat cgacggcgtc     3420

cgtgtcctct ggactgctcc agggcgtgcc gcccgtgatg agacggcttt tcgccacgct     3480

ttgactgtgg gataccagtt aaaagcggct ggtgagcgcc taaaagacac caagatcatc     3540

gacgcctacg agcgtgccta caccgtcgct caggcggtcg gagcagacgg ccgtgagcct     3600

gatctgccgc cgatgcgtga ccgccagacg atggcgcgac gtgtgcgcgg ctacgtcgct     3660

aaaggccagc cagtcgtccc tgctcgtcag acagagacgc agagcagccg agggcgaaaa     3720

gctctggcca ctatgggaag acgtggcggt aaaaaggccg cagaacgctg gaaagaccca     3780

aacagtgagt acgcccgagc acagcgagaa aaactagcta agtccagtca acgacaagct     3840

aggaaagcta aaggaaatcg cttgaccatt gcaggttggt ttatgactgt tgagggagag     3900

actggctcgt ggcgacaatc aatgaagcta tgtctgaatt tagcgtgtca cgtcagaccg     3960

tgaatagagc acttaagtct gcgggcattg aacttccacg aggacgccgt aaagcttccc     4020

agtaaatgtg ccatctcgta ggcagaaaac ggttcccccc gtaggggtct ctctcttggc     4080

ctcctttcta ggtcgggctg attgctcttg aagctctcta ggggggctca caccataggc     4140

agataacggt tccccaccgg ctcacctcgt aagcgcacaa ggactgctcc caatgccgca     4200

agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga     4260

aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa     4320

gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg     4380

ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga     4440

agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat     4500

caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc     4560

acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga     4620

caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt     4680

ttgtcaagac cgacctgtcc ggtgccctga atgaactcca agacgaggca gcgcggctat     4740

cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg     4800

gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg     4860

ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc     4920

cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga     4980

tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag     5040

ccgaactgtt cgccaggctc aaggcgcgga tgcccgacgg cgaggatctc gtcgtgaccc     5100

atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg     5160

actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata     5220

ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg     5280

ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac     5340

tctggggttc gctagaggat cgatcctttt taacccatca catatacctg ccgttcacta     5400

ttatttagtg aaatgagata ttatgatatt ttctgaattg tgattaaaaa ggcaacttta     5460

tgcccatgca acagaaacta taaaaaatac agagaatgaa aagaaacaga tagatttttt     5520

agttctttag gcccgtagtc tgcaaatcct tttatgattt tctatcaaac aaaagaggaa     5580

aatagaccag ttgcaatcca aacgagagtc taacgacgcg aggctggatg gccttcccca     5640

ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca     5700

ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc     5760

taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat     5820

ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc     5880

gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa     5940

cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca     6000

aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg     6060

catctcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag     6120

gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga     6180

gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt     6240

cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat     6300

gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt     6360

aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc     6420

cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat     6480

cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta     6540

cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag     6600

aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga     6660

catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt     6720

cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct     6780

gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg     6840

tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat     6900

gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga     6960

tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg     7020

cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta     7080

tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc     7140

aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag     7200

catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac     7260

caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc     7320

ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt     7380

aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc     7440

gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga     7500

cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta     7560

ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta     7620

tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga     7680

tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg     7740

cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag     7800

tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc     7860

tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact     7920

tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt     7980

cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta     8040

ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta     8100

tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc     8160

gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat     8220

agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt     8280

atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg     8340

tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca     8400

gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta     8460

agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg     8520

cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact     8580

ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg     8640

ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt     8700

actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga     8760

ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc     8820

atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa     8880

caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt     8940

attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa     9000


<210>  34
<211>  8198
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10433; beta actin hairpin - coat protein


<220>
<221>  promoter
<222>  (10)..(28)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (32)..(39)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (41)..(334)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (334)..(341)
<223>  restriction endonuclease PmeI site

<220>
<221>  misc_feature
<222>  (498)..(793)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (868)..(886)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (902)..(949)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  misc_feature
<222>  (1470)..(4812)
<223>  bacteriophage T7 transcription terminator

<400>  34
ttccgaaatt aatacgactc actataggga ggcgatcgcg cacgaggttt ttctgtctag       60

tgagcagtgt ccaacctcaa aagacaacat gtgtgacgac gatgtagcgg ctcttgtcgt      120

agacaatgga tccggtatgt gcaaagccgg tttcgcagga gatgacgcac cccgtgccgt      180

cttcccctcg atcgtcggtc gcccaaggca tcaaggagtc atggtcggta tgggacaaaa      240

ggactcatac gtaggagatg aagcccaaag caaaagaggt atcctcaccc tgaaataccc      300

catcgaacac ggtatcatca ccaactggga tgagtttaaa ccctctagct gctttacaaa      360

gtactggttc cctttccagc gggatgcttt atctaaacgc aatgagagag gtattcctca      420

ggccacatcg cttcctagtt ccgctgggat ccatcgttgg cggccgaagc cgccattcca      480

tagtgagttc tggcgcgcct catcccagtt ggtgatgata ccgtgttcga tggggtattt      540

cagggtgagg atacctcttt tgctttgggc ttcatctcct acgtatgagt ccttttgtcc      600

cataccgacc atgactcctt gatgccttgg gcgaccgacg atcgagggga agacggcacg      660

gggtgcgtca tctcctgcga aaccggcttt gcacataccg gatccattgt ctacgacaag      720

agccgctaca tcgtcgtcac acatgttgtc ttttgaggtt ggacactgct cactagacag      780

aaaaacctcg tgccggaccg aatacccggt ctgaacgagg gcggccgcgg tacccaagaa      840

gtacttagag ttaattaagg agttcaaaca tgaggatcac ccatgtcgaa gctcccacac      900

cctagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg      960

aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg     1020

ctccaagtag cgaagcgagc aggactgggc ggcgggcatg caccattcct tgcggcggcg     1080

gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga     1140

gagcgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg     1200

ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag     1260

gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg     1320

atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact     1380

ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac     1440

gcgctgggct acgtcttgct ggcgttcgcc atggtcgtca cagagctgga agcggcagcg     1500

agaattatcc gcgatcgtgg cgcggtgccc gcaggcatga caaacatcgt aaatgccgcg     1560

tttcgtgtgg ccgtggccgc ccaggacgtg tcagcgccgc caccacctgc accgaatcgg     1620

cagcagcgtc gcgcgtcgaa aaagcgcaca ggcggcaaga agcgataagc tgcacgaata     1680

cctgaaaaat gttgaacgcc ccgtgagcgg taactcacag ggcgtcggct aacccccagt     1740

ccaaacctgg gagaaagcgc tcaaaaatga ctctagcgga ttcacgagac attgacacac     1800

cggcctggaa attttccgct gatctgttcg acacccatcc cgagctcgcg ctgcgatcac     1860

gtggctggac gagcgaagac cgccgcgaat tcctcgctca cctgggcaga gaaaatttcc     1920

agggcagcaa gacccgcgac ttcgccagcg cttggatcaa agacccggac acgggagaaa     1980

cacagccgaa gttataccga gttggttcaa aatcgcttgc ccggtgccag tatgttgctc     2040

tgacgcacgc gcagcacgca gccgtgcttg tcctggacat tgatgtgccg agccaccagg     2100

ccggcgggaa aatcgagcac gtaaaccccg aggtctacgc gattttggag cgctgggcac     2160

gcctggaaaa agcgccagct tggatcggcg tgaatccact gagcgggaaa tgccagctca     2220

tctggctcat tgatccggtg tatgccgcag caggcatgag cagcccgaat atgcgcctgc     2280

tggctgcaac gaccgaggaa atgacccgcg ttttcggcgc tgaccaggct ttttcacata     2340

ggctgagccg gtggccactg cacgtctccg acgatcccac cgcgtaccgc tggcatgccc     2400

agcacaatcg cgtggatcgc ctagctgatc ttatggaggt tgctcgcatg atctcaggca     2460

cagaaaaacc taaaaaacgc tatgagcagg agttttctag cggacgggca cgtatcgaag     2520

cggcaagaaa agccactgcg gaagcaaaag cacttgccac gcttgaagca agcctgccga     2580

gcgccgctga agcgtctgga gagctgatcg acggcgtccg tgtcctctgg actgctccag     2640

ggcgtgccgc ccgtgatgag acggcttttc gccacgcttt gactgtggga taccagttaa     2700

aagcggctgg tgagcgccta aaagacacca agatcatcga cgcctacgag cgtgcctaca     2760

ccgtcgctca ggcggtcgga gcagacggcc gtgagcctga tctgccgccg atgcgtgacc     2820

gccagacgat ggcgcgacgt gtgcgcggct acgtcgctaa aggccagcca gtcgtccctg     2880

ctcgtcagac agagacgcag agcagccgag ggcgaaaagc tctggccact atgggaagac     2940

gtggcggtaa aaaggccgca gaacgctgga aagacccaaa cagtgagtac gcccgagcac     3000

agcgagaaaa actagctaag tccagtcaac gacaagctag gaaagctaaa ggaaatcgct     3060

tgaccattgc aggttggttt atgactgttg agggagagac tggctcgtgg cgacaatcaa     3120

tgaagctatg tctgaattta gcgtgtcacg tcagaccgtg aatagagcac ttaagtctgc     3180

gggcattgaa cttccacgag gacgccgtaa agcttcccag taaatgtgcc atctcgtagg     3240

cagaaaacgg ttccccccgt aggggtctct ctcttggcct cctttctagg tcgggctgat     3300

tgctcttgaa gctctctagg ggggctcaca ccataggcag ataacggttc cccaccggct     3360

cacctcgtaa gcgcacaagg actgctccca atgccgcaag cactcagggc gcaagggctg     3420

ctaaaggaag cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa     3480

tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc     3540

ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc     3600

ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat     3660

ggctttcttg ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg     3720

atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg     3780

ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc     3840

cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg     3900

tgccctgaat gaactccaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt     3960

tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg     4020

cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat     4080

catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca     4140

ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca     4200

ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa     4260

ggcgcggatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa     4320

tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc     4380

ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga     4440

atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc     4500

cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc tagaggatcg     4560

atccttttta acccatcaca tatacctgcc gttcactatt atttagtgaa atgagatatt     4620

atgatatttt ctgaattgtg attaaaaagg caactttatg cccatgcaac agaaactata     4680

aaaaatacag agaatgaaaa gaaacagata gattttttag ttctttaggc ccgtagtctg     4740

caaatccttt tatgattttc tatcaaacaa aagaggaaaa tagaccagtt gcaatccaaa     4800

cgagagtcta acgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg     4860

cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca     4920

gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca ttggaccgct     4980

gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt     5040

aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc     5100

cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac tccaagaatt     5160

ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg gcagaacata     5220

tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag cgttgggtcc     5280

tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt     5340

tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc     5400

tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa     5460

agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga     5520

tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc     5580

tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt     5640

tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc     5700

atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa     5760

caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg     5820

gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac     5880

cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc     5940

tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga     6000

caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag     6060

tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac     6120

tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca     6180

tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc     6240

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg     6300

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt     6360

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa     6420

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct     6480

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc     6540

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg     6600

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct     6660

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag     6720

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga     6780

agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga     6840

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg     6900

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag     6960

aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag     7020

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat     7080

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct     7140

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac     7200

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa     7260

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg     7320

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt     7380

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca     7440

ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt     7500

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct     7560

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg     7620

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg     7680

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg     7740

cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa     7800

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt     7860

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt     7920

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt     7980

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca     8040

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat     8100

ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata     8160

aaaataggcg tatcacgagg ccctttcgtc ttcaagaa                             8198


<210>  35
<211>  9327
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10434; beta actin hairpin + coat protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (374)..(667)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (667)..(674)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (832)..(1126)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (1201)..(1219)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (1235)..(1282)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1602)..(1620)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1693)..(2082)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (2115)..(2162)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  misc_feature
<222>  (2599)..(5941)
<223>  synthetic DNA (SEQ ID NO: 30)

<400>  35
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta tgtgcaaagc      480

cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg gtcgcccaag      540

gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag atgaagccca      600

aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca tcaccaactg      660

ggatgagttt aaaccctcta gctgctttac aaagtactgg ttccctttcc agcgggatgc      720

tttatctaaa cgcaatgaga gaggtattcc tcaggccaca tcgcttccta gttccgctgg      780

gatccatcgt tggcggccga agccgccatt ccatagtgag ttctggcgcg cctcatccca      840

gttggtgatg ataccgtgtt cgatggggta tttcagggtg aggatacctc ttttgctttg      900

ggcttcatct cctacgtatg agtccttttg tcccataccg accatgactc cttgatgcct      960

tgggcgaccg acgatcgagg ggaagacggc acggggtgcg tcatctcctg cgaaaccggc     1020

tttgcacata ccggatccat tgtctacgac aagagccgct acatcgtcgt cacacatgtt     1080

gtcttttgag gttggacact gctcactaga cagaaaaacc tcgtgccgga ccgaataccc     1140

ggtctgaacg agggcggccg cggtacccaa gaagtactta gagttaatta aggagttcaa     1200

acatgaggat cacccatgtc gaagctccca caccctagca taaccccttg gggcctctaa     1260

acgggtcttg aggggttttt tgctgaaagg aggaactata tccgcacagg acgggtgtgg     1320

tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc     1380

gggcatgcat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg ctagcgctat     1440

atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac cgctttggcc     1500

gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg atcatggcga     1560

ccacacccgt cctgtggatc cagatctcga tcccgcgaaa ttaatacgac tcactatagg     1620

gagaccacaa cggtttccct ctagatcaca agtttgtaca aaaaagcagg ctaagaagga     1680

gatatacata tggcgtctaa ctttacccaa ttcgttctgg ttgataacgg cggtacgggt     1740

gacgttaccg tagctccgtc caacttcgcc aacggtgttg cggaatggat tagctctaac     1800

agccgctctc aggcctacaa agtcacgtgc tccgttcgtc agtctagcgc gcagaatcgc     1860

aaatacacca tcaaagttga agtaccgaaa gtcgcaacgc agaccgtagg cggcgtagaa     1920

ctcccagttg cggcctggcg ctcttacctc aacatggaac tgactattcc gatttttgcg     1980

acgaactccg actgcgaact gattgttaag gcaatgcagg gcctgctgaa agacggtaat     2040

ccgatcccat ctgcaatcgc tgctaactct ggcatttact aataagcgga cgcgctgcca     2100

ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt     2160

tgctgaaagg aggaactata tccggcatgc accattcctt gcggcggcgg tgctcaacgg     2220

cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc     2280

gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat     2340

cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc     2400

gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc     2460

gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac     2520

caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta     2580

cgtcttgctg gcgttcgcca tggtcgtcac agagctggaa gcggcagcga gaattatccg     2640

cgatcgtggc gcggtgcccg caggcatgac aaacatcgta aatgccgcgt ttcgtgtggc     2700

cgtggccgcc caggacgtgt cagcgccgcc accacctgca ccgaatcggc agcagcgtcg     2760

cgcgtcgaaa aagcgcacag gcggcaagaa gcgataagct gcacgaatac ctgaaaaatg     2820

ttgaacgccc cgtgagcggt aactcacagg gcgtcggcta acccccagtc caaacctggg     2880

agaaagcgct caaaaatgac tctagcggat tcacgagaca ttgacacacc ggcctggaaa     2940

ttttccgctg atctgttcga cacccatccc gagctcgcgc tgcgatcacg tggctggacg     3000

agcgaagacc gccgcgaatt cctcgctcac ctgggcagag aaaatttcca gggcagcaag     3060

acccgcgact tcgccagcgc ttggatcaaa gacccggaca cgggagaaac acagccgaag     3120

ttataccgag ttggttcaaa atcgcttgcc cggtgccagt atgttgctct gacgcacgcg     3180

cagcacgcag ccgtgcttgt cctggacatt gatgtgccga gccaccaggc cggcgggaaa     3240

atcgagcacg taaaccccga ggtctacgcg attttggagc gctgggcacg cctggaaaaa     3300

gcgccagctt ggatcggcgt gaatccactg agcgggaaat gccagctcat ctggctcatt     3360

gatccggtgt atgccgcagc aggcatgagc agcccgaata tgcgcctgct ggctgcaacg     3420

accgaggaaa tgacccgcgt tttcggcgct gaccaggctt tttcacatag gctgagccgg     3480

tggccactgc acgtctccga cgatcccacc gcgtaccgct ggcatgccca gcacaatcgc     3540

gtggatcgcc tagctgatct tatggaggtt gctcgcatga tctcaggcac agaaaaacct     3600

aaaaaacgct atgagcagga gttttctagc ggacgggcac gtatcgaagc ggcaagaaaa     3660

gccactgcgg aagcaaaagc acttgccacg cttgaagcaa gcctgccgag cgccgctgaa     3720

gcgtctggag agctgatcga cggcgtccgt gtcctctgga ctgctccagg gcgtgccgcc     3780

cgtgatgaga cggcttttcg ccacgctttg actgtgggat accagttaaa agcggctggt     3840

gagcgcctaa aagacaccaa gatcatcgac gcctacgagc gtgcctacac cgtcgctcag     3900

gcggtcggag cagacggccg tgagcctgat ctgccgccga tgcgtgaccg ccagacgatg     3960

gcgcgacgtg tgcgcggcta cgtcgctaaa ggccagccag tcgtccctgc tcgtcagaca     4020

gagacgcaga gcagccgagg gcgaaaagct ctggccacta tgggaagacg tggcggtaaa     4080

aaggccgcag aacgctggaa agacccaaac agtgagtacg cccgagcaca gcgagaaaaa     4140

ctagctaagt ccagtcaacg acaagctagg aaagctaaag gaaatcgctt gaccattgca     4200

ggttggttta tgactgttga gggagagact ggctcgtggc gacaatcaat gaagctatgt     4260

ctgaatttag cgtgtcacgt cagaccgtga atagagcact taagtctgcg ggcattgaac     4320

ttccacgagg acgccgtaaa gcttcccagt aaatgtgcca tctcgtaggc agaaaacggt     4380

tccccccgta ggggtctctc tcttggcctc ctttctaggt cgggctgatt gctcttgaag     4440

ctctctaggg gggctcacac cataggcaga taacggttcc ccaccggctc acctcgtaag     4500

cgcacaagga ctgctcccaa tgccgcaagc actcagggcg caagggctgc taaaggaagc     4560

ggaacacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact     4620

gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc     4680

ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag     4740

ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc     4800

cgccaaggat ctgatggcgc aggggatcaa gatctgatca agagacagga tgaggatcgt     4860

ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc     4920

tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc     4980

tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg     5040

aactccaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag     5100

ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg     5160

ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg     5220

caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac     5280

atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg     5340

acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcggatgc     5400

ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg     5460

aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc     5520

aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc     5580

gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc     5640

ttcttgacga gttcttctga gcgggactct ggggttcgct agaggatcga tcctttttaa     5700

cccatcacat atacctgccg ttcactatta tttagtgaaa tgagatatta tgatattttc     5760

tgaattgtga ttaaaaaggc aactttatgc ccatgcaaca gaaactataa aaaatacaga     5820

gaatgaaaag aaacagatag attttttagt tctttaggcc cgtagtctgc aaatcctttt     5880

atgattttct atcaaacaaa agaggaaaat agaccagttg caatccaaac gagagtctaa     5940

cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga     6000

tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc     6060

aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg atcgtcacgg     6120

cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc     6180

tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct     6240

gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca     6300

attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc     6360

cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt     6420

gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg     6480

ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc     6540

tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac     6600

gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct     6660

accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt     6720

tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg     6780

ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat     6840

tacccccatg aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac     6900

cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa     6960

cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga     7020

gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca     7080

gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca     7140

gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga     7200

tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac     7260

catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct     7320

tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca     7380

gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac     7440

atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt     7500

ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg     7560

cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc     7620

tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc     7680

gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc     7740

aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac     7800

tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt     7860

aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct     7920

aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc     7980

ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt     8040

ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg     8100

atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc     8160

atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa     8220

tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag     8280

gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg     8340

tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga     8400

gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag     8460

cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa     8520

gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc     8580

atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca     8640

aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg     8700

atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat     8760

aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc     8820

aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg     8880

gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg     8940

gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt     9000

gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca     9060

ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata     9120

ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac     9180

atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa     9240

gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt     9300

atcacgaggc cctttcgtct tcaagaa                                         9327


<210>  36
<211>  8531
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10435; beta actin hairpin - coat protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (374)..(667)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (667)..(674)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (832)..(1126)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (1201)..(1219)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (1235)..(1282)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  misc_feature
<222>  (1803)..(5145)
<223>  synthetic DNA (SEQ ID NO: 30)

<400>  36
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta tgtgcaaagc      480

cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg gtcgcccaag      540

gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag atgaagccca      600

aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca tcaccaactg      660

ggatgagttt aaaccctcta gctgctttac aaagtactgg ttccctttcc agcgggatgc      720

tttatctaaa cgcaatgaga gaggtattcc tcaggccaca tcgcttccta gttccgctgg      780

gatccatcgt tggcggccga agccgccatt ccatagtgag ttctggcgcg cctcatccca      840

gttggtgatg ataccgtgtt cgatggggta tttcagggtg aggatacctc ttttgctttg      900

ggcttcatct cctacgtatg agtccttttg tcccataccg accatgactc cttgatgcct      960

tgggcgaccg acgatcgagg ggaagacggc acggggtgcg tcatctcctg cgaaaccggc     1020

tttgcacata ccggatccat tgtctacgac aagagccgct acatcgtcgt cacacatgtt     1080

gtcttttgag gttggacact gctcactaga cagaaaaacc tcgtgccgga ccgaataccc     1140

ggtctgaacg agggcggccg cggtacccaa gaagtactta gagttaatta aggagttcaa     1200

acatgaggat cacccatgtc gaagctccca caccctagca taaccccttg gggcctctaa     1260

acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg     1320

gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg     1380

ggcggcgggc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg     1440

ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc     1500

ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg     1560

actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc     1620

ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga     1680

atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag     1740

aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc     1800

gccatggtcg tcacagagct ggaagcggca gcgagaatta tccgcgatcg tggcgcggtg     1860

cccgcaggca tgacaaacat cgtaaatgcc gcgtttcgtg tggccgtggc cgcccaggac     1920

gtgtcagcgc cgccaccacc tgcaccgaat cggcagcagc gtcgcgcgtc gaaaaagcgc     1980

acaggcggca agaagcgata agctgcacga atacctgaaa aatgttgaac gccccgtgag     2040

cggtaactca cagggcgtcg gctaaccccc agtccaaacc tgggagaaag cgctcaaaaa     2100

tgactctagc ggattcacga gacattgaca caccggcctg gaaattttcc gctgatctgt     2160

tcgacaccca tcccgagctc gcgctgcgat cacgtggctg gacgagcgaa gaccgccgcg     2220

aattcctcgc tcacctgggc agagaaaatt tccagggcag caagacccgc gacttcgcca     2280

gcgcttggat caaagacccg gacacgggag aaacacagcc gaagttatac cgagttggtt     2340

caaaatcgct tgcccggtgc cagtatgttg ctctgacgca cgcgcagcac gcagccgtgc     2400

ttgtcctgga cattgatgtg ccgagccacc aggccggcgg gaaaatcgag cacgtaaacc     2460

ccgaggtcta cgcgattttg gagcgctggg cacgcctgga aaaagcgcca gcttggatcg     2520

gcgtgaatcc actgagcggg aaatgccagc tcatctggct cattgatccg gtgtatgccg     2580

cagcaggcat gagcagcccg aatatgcgcc tgctggctgc aacgaccgag gaaatgaccc     2640

gcgttttcgg cgctgaccag gctttttcac ataggctgag ccggtggcca ctgcacgtct     2700

ccgacgatcc caccgcgtac cgctggcatg cccagcacaa tcgcgtggat cgcctagctg     2760

atcttatgga ggttgctcgc atgatctcag gcacagaaaa acctaaaaaa cgctatgagc     2820

aggagttttc tagcggacgg gcacgtatcg aagcggcaag aaaagccact gcggaagcaa     2880

aagcacttgc cacgcttgaa gcaagcctgc cgagcgccgc tgaagcgtct ggagagctga     2940

tcgacggcgt ccgtgtcctc tggactgctc cagggcgtgc cgcccgtgat gagacggctt     3000

ttcgccacgc tttgactgtg ggataccagt taaaagcggc tggtgagcgc ctaaaagaca     3060

ccaagatcat cgacgcctac gagcgtgcct acaccgtcgc tcaggcggtc ggagcagacg     3120

gccgtgagcc tgatctgccg ccgatgcgtg accgccagac gatggcgcga cgtgtgcgcg     3180

gctacgtcgc taaaggccag ccagtcgtcc ctgctcgtca gacagagacg cagagcagcc     3240

gagggcgaaa agctctggcc actatgggaa gacgtggcgg taaaaaggcc gcagaacgct     3300

ggaaagaccc aaacagtgag tacgcccgag cacagcgaga aaaactagct aagtccagtc     3360

aacgacaagc taggaaagct aaaggaaatc gcttgaccat tgcaggttgg tttatgactg     3420

ttgagggaga gactggctcg tggcgacaat caatgaagct atgtctgaat ttagcgtgtc     3480

acgtcagacc gtgaatagag cacttaagtc tgcgggcatt gaacttccac gaggacgccg     3540

taaagcttcc cagtaaatgt gccatctcgt aggcagaaaa cggttccccc cgtaggggtc     3600

tctctcttgg cctcctttct aggtcgggct gattgctctt gaagctctct aggggggctc     3660

acaccatagg cagataacgg ttccccaccg gctcacctcg taagcgcaca aggactgctc     3720

ccaatgccgc aagcactcag ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc     3780

cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag     3840

ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct     3900

agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg     3960

taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg     4020

gcgcagggga tcaagatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca     4080

agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg     4140

ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg     4200

cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aagacgaggc     4260

agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt     4320

cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc     4380

atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca     4440

tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc     4500

acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg     4560

gctcgcgcca gccgaactgt tcgccaggct caaggcgcgg atgcccgacg gcgaggatct     4620

cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc     4680

tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc     4740

tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta     4800

cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt     4860

ctgagcggga ctctggggtt cgctagagga tcgatccttt ttaacccatc acatatacct     4920

gccgttcact attatttagt gaaatgagat attatgatat tttctgaatt gtgattaaaa     4980

aggcaacttt atgcccatgc aacagaaact ataaaaaata cagagaatga aaagaaacag     5040

atagattttt tagttcttta ggcccgtagt ctgcaaatcc ttttatgatt ttctatcaaa     5100

caaaagagga aaatagacca gttgcaatcc aaacgagagt ctaacgacgc gaggctggat     5160

ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc     5220

catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc     5280

tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt atgccgcctc     5340

ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct     5400

ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg     5460

cacctcgcta acggattcac cactccaaga attggagcca atcaattctt gcggagaact     5520

gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat ctccagcagc     5580

cgcacgcggc gcatctcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc     5640

ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca     5700

ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca     5760

acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc     5820

tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct     5880

acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc     5940

atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca     6000

gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga     6060

aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc     6120

cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat     6180

gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc     6240

ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc     6300

acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt     6360

gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact     6420

ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa     6480

taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca     6540

ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg     6600

taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc     6660

agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc     6720

cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac     6780

tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc     6840

tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata     6900

gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc     6960

acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca     7020

acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag     7080

cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta     7140

gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg     7200

gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc     7260

agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt     7320

ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa     7380

ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat     7440

atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga     7500

tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac     7560

gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg     7620

ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg     7680

caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt     7740

cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct     7800

cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat     7860

cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta     7920

agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca     7980

tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat     8040

agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac     8100

atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa     8160

ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt     8220

cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg     8280

caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat     8340

attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt     8400

agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct     8460

aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc     8520

gtcttcaaga a                                                          8531


<210>  37
<211>  9520
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10436; beta actin hairpin + coat protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (374)..(667)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (665)..(672)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (735)..(753)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (832)..(1126)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (1201)..(1219)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (1235)..(1282)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  promoter
<222>  (1608)..(1626)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  gene
<222>  (1699)..(2088)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  terminator
<222>  (2121)..(2168)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  misc_feature
<222>  (2792)..(6134)
<223>  synthetic DNA (SEQ ID NO: 30)

<400>  37
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta tgtgcaaagc      480

cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg gtcgcccaag      540

gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag atgaagccca      600

aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca tcaccaactg      660

ggatgagttt aaaccctcta gctgctttac aaagtactgg ttccctttcc agcgggatgc      720

tttatctaaa cgcaacatga ggatcaccca tgtcgccaca tcgcttccta gttccgctgg      780

gatccatcgt tggcggccga agccgccatt ccatagtgag ttctggcgcg cctcatccca      840

gttggtgatg ataccgtgtt cgatggggta tttcagggtg aggatacctc ttttgctttg      900

ggcttcatct cctacgtatg agtccttttg tcccataccg accatgactc cttgatgcct      960

tgggcgaccg acgatcgagg ggaagacggc acggggtgcg tcatctcctg cgaaaccggc     1020

tttgcacata ccggatccat tgtctacgac aagagccgct acatcgtcgt cacacatgtt     1080

gtcttttgag gttggacact gctcactaga cagaaaaacc tcgtgccgga ccgaataccc     1140

ggtctgaacg agggcggccg cggtacccaa gaagtactta gagttaatta aggagttcaa     1200

acatgaggat cacccatgtc gaagctccca caccctagca taaccccttg gggcctctaa     1260

acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg     1320

gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg     1380

ggcggcgggc atgcatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag     1440

cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct     1500

ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca     1560

tggcgaccac acccgtcctg tggatccaga tctcgatccc gcgaaattaa tacgactcac     1620

tatagggaga ccacaacggt ttccctctag atcacaagtt tgtacaaaaa agcaggctaa     1680

gaaggagata tacatatggc gtctaacttt acccaattcg ttctggttga taacggcggt     1740

acgggtgacg ttaccgtagc tccgtccaac ttcgccaacg gtgttgcgga atggattagc     1800

tctaacagcc gctctcaggc ctacaaagtc acgtgctccg ttcgtcagtc tagcgcgcag     1860

aatcgcaaat acaccatcaa agttgaagta ccgaaagtcg caacgcagac cgtaggcggc     1920

gtagaactcc cagttgcggc ctggcgctct tacctcaaca tggaactgac tattccgatt     1980

tttgcgacga actccgactg cgaactgatt gttaaggcaa tgcagggcct gctgaaagac     2040

ggtaatccga tcccatctgc aatcgctgct aactctggca tttactaata agcggacgcg     2100

ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg     2160

gttttttgct gaaaggagga actatatccg gcatgcatcg tccattccga cagcatcgcc     2220

agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt     2280

ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga     2340

gccactatcg actacgcgat catggcgacc acacccgtcc tgtaccattc cttgcggcgg     2400

cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg     2460

gagagcgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc     2520

ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac     2580

aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga     2640

tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca     2700

ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg     2760

acgcgctggg ctacgtcttg ctggcgttcg ccatggtcgt cacagagctg gaagcggcag     2820

cgagaattat ccgcgatcgt ggcgcggtgc ccgcaggcat gacaaacatc gtaaatgccg     2880

cgtttcgtgt ggccgtggcc gcccaggacg tgtcagcgcc gccaccacct gcaccgaatc     2940

ggcagcagcg tcgcgcgtcg aaaaagcgca caggcggcaa gaagcgataa gctgcacgaa     3000

tacctgaaaa atgttgaacg ccccgtgagc ggtaactcac agggcgtcgg ctaaccccca     3060

gtccaaacct gggagaaagc gctcaaaaat gactctagcg gattcacgag acattgacac     3120

accggcctgg aaattttccg ctgatctgtt cgacacccat cccgagctcg cgctgcgatc     3180

acgtggctgg acgagcgaag accgccgcga attcctcgct cacctgggca gagaaaattt     3240

ccagggcagc aagacccgcg acttcgccag cgcttggatc aaagacccgg acacgggaga     3300

aacacagccg aagttatacc gagttggttc aaaatcgctt gcccggtgcc agtatgttgc     3360

tctgacgcac gcgcagcacg cagccgtgct tgtcctggac attgatgtgc cgagccacca     3420

ggccggcggg aaaatcgagc acgtaaaccc cgaggtctac gcgattttgg agcgctgggc     3480

acgcctggaa aaagcgccag cttggatcgg cgtgaatcca ctgagcggga aatgccagct     3540

catctggctc attgatccgg tgtatgccgc agcaggcatg agcagcccga atatgcgcct     3600

gctggctgca acgaccgagg aaatgacccg cgttttcggc gctgaccagg ctttttcaca     3660

taggctgagc cggtggccac tgcacgtctc cgacgatccc accgcgtacc gctggcatgc     3720

ccagcacaat cgcgtggatc gcctagctga tcttatggag gttgctcgca tgatctcagg     3780

cacagaaaaa cctaaaaaac gctatgagca ggagttttct agcggacggg cacgtatcga     3840

agcggcaaga aaagccactg cggaagcaaa agcacttgcc acgcttgaag caagcctgcc     3900

gagcgccgct gaagcgtctg gagagctgat cgacggcgtc cgtgtcctct ggactgctcc     3960

agggcgtgcc gcccgtgatg agacggcttt tcgccacgct ttgactgtgg gataccagtt     4020

aaaagcggct ggtgagcgcc taaaagacac caagatcatc gacgcctacg agcgtgccta     4080

caccgtcgct caggcggtcg gagcagacgg ccgtgagcct gatctgccgc cgatgcgtga     4140

ccgccagacg atggcgcgac gtgtgcgcgg ctacgtcgct aaaggccagc cagtcgtccc     4200

tgctcgtcag acagagacgc agagcagccg agggcgaaaa gctctggcca ctatgggaag     4260

acgtggcggt aaaaaggccg cagaacgctg gaaagaccca aacagtgagt acgcccgagc     4320

acagcgagaa aaactagcta agtccagtca acgacaagct aggaaagcta aaggaaatcg     4380

cttgaccatt gcaggttggt ttatgactgt tgagggagag actggctcgt ggcgacaatc     4440

aatgaagcta tgtctgaatt tagcgtgtca cgtcagaccg tgaatagagc acttaagtct     4500

gcgggcattg aacttccacg aggacgccgt aaagcttccc agtaaatgtg ccatctcgta     4560

ggcagaaaac ggttcccccc gtaggggtct ctctcttggc ctcctttcta ggtcgggctg     4620

attgctcttg aagctctcta ggggggctca caccataggc agataacggt tccccaccgg     4680

ctcacctcgt aagcgcacaa ggactgctcc caatgccgca agcactcagg gcgcaagggc     4740

tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg accccggatg     4800

aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta     4860

gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa     4920

ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg     4980

atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca     5040

ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct     5100

tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc     5160

gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc     5220

ggtgccctga atgaactcca agacgaggca gcgcggctat cgtggctggc cacgacgggc     5280

gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg     5340

ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc     5400

atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac     5460

caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat     5520

caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc     5580

aaggcgcgga tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg     5640

aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg     5700

gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc     5760

gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc     5820

gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gctagaggat     5880

cgatcctttt taacccatca catatacctg ccgttcacta ttatttagtg aaatgagata     5940

ttatgatatt ttctgaattg tgattaaaaa ggcaacttta tgcccatgca acagaaacta     6000

taaaaaatac agagaatgaa aagaaacaga tagatttttt agttctttag gcccgtagtc     6060

tgcaaatcct tttatgattt tctatcaaac aaaagaggaa aatagaccag ttgcaatcca     6120

aacgagagtc taacgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc     6180

ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat     6240

cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cattggaccg     6300

ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt     6360

gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg     6420

gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa     6480

ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca     6540

tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc agcgttgggt     6600

cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg     6660

gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct     6720

gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt     6780

aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag     6840

gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac     6900

cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac     6960

gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt     7020

tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacca     7080

aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc     7140

tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg     7200

accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc     7260

tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca     7320

gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc     7380

agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt     7440

actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg     7500

catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg     7560

gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa     7620

cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc     7680

gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc     7740

aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag     7800

ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct     7860

cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta     7920

ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc     7980

cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc     8040

agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt     8100

gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct     8160

gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc     8220

tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca     8280

agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta     8340

agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa     8400

atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg     8460

cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg     8520

actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc     8580

aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc     8640

cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa     8700

ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc     8760

cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg     8820

ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc     8880

cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat     8940

ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg     9000

tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc     9060

ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg     9120

aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat     9180

gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg     9240

gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg     9300

ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct     9360

catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac     9420

atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta     9480

taaaaatagg cgtatcacga ggccctttcg tcttcaagaa                           9520


<210>  38
<211>  8718
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10437; beta actin hairpin - coat protein


<220>
<221>  promoter
<222>  (214)..(232)
<223>  bacteriophage T7 gene 1 promoter

<220>
<221>  misc_feature
<222>  (317)..(335)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (365)..(372)
<223>  restriction endonuclease AsiSI recognition site

<220>
<221>  misc_feature
<222>  (374)..(667)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (667)..(674)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (735)..(753)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (832)..(1126)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (1201)..(1219)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (1235)..(1282)
<223>  bacteriophage T7 transcription terminator

<220>
<221>  misc_feature
<222>  (1990)..(5332)
<223>  synthetic DNA (SEQ ID NO: 30)

<400>  38
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg      120

caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt      180

gcgggatgaa ttcagatctc gatcccgcga aattaatacg actcactata gggagaccac      240

aacggtttcc ctctagatca caagtttgta caaaaaagca ggctaagaag gagatataca      300

tacgccggcc attcaaacat gaggattacc catgtattta aatacccatg tccaggcgcg      360

ctccgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct caaaagacaa      420

catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta tgtgcaaagc      480

cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg gtcgcccaag      540

gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag atgaagccca      600

aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca tcaccaactg      660

ggatgagttt aaaccctcta gctgctttac aaagtactgg ttccctttcc agcgggatgc      720

tttatctaaa cgcaacatga ggatcaccca tgtcgccaca tcgcttccta gttccgctgg      780

gatccatcgt tggcggccga agccgccatt ccatagtgag ttctggcgcg cctcatccca      840

gttggtgatg ataccgtgtt cgatggggta tttcagggtg aggatacctc ttttgctttg      900

ggcttcatct cctacgtatg agtccttttg tcccataccg accatgactc cttgatgcct      960

tgggcgaccg acgatcgagg ggaagacggc acggggtgcg tcatctcctg cgaaaccggc     1020

tttgcacata ccggatccat tgtctacgac aagagccgct acatcgtcgt cacacatgtt     1080

gtcttttgag gttggacact gctcactaga cagaaaaacc tcgtgccgga ccgaataccc     1140

ggtctgaacg agggcggccg cggtacccaa gaagtactta gagttaatta aggagttcaa     1200

acatgaggat cacccatgtc gaagctccca caccctagca taaccccttg gggcctctaa     1260

acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg     1320

gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg     1380

ggcggcgggc atgcatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag     1440

cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct     1500

ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca     1560

tggcgaccac acccgtcctg taccattcct tgcggcggcg gtgctcaacg gcctcaacct     1620

actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgac cgatgccctt     1680

gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc     1740

acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt     1800

cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt     1860

attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt     1920

cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct     1980

ggcgttcgcc atggtcgtca cagagctgga agcggcagcg agaattatcc gcgatcgtgg     2040

cgcggtgccc gcaggcatga caaacatcgt aaatgccgcg tttcgtgtgg ccgtggccgc     2100

ccaggacgtg tcagcgccgc caccacctgc accgaatcgg cagcagcgtc gcgcgtcgaa     2160

aaagcgcaca ggcggcaaga agcgataagc tgcacgaata cctgaaaaat gttgaacgcc     2220

ccgtgagcgg taactcacag ggcgtcggct aacccccagt ccaaacctgg gagaaagcgc     2280

tcaaaaatga ctctagcgga ttcacgagac attgacacac cggcctggaa attttccgct     2340

gatctgttcg acacccatcc cgagctcgcg ctgcgatcac gtggctggac gagcgaagac     2400

cgccgcgaat tcctcgctca cctgggcaga gaaaatttcc agggcagcaa gacccgcgac     2460

ttcgccagcg cttggatcaa agacccggac acgggagaaa cacagccgaa gttataccga     2520

gttggttcaa aatcgcttgc ccggtgccag tatgttgctc tgacgcacgc gcagcacgca     2580

gccgtgcttg tcctggacat tgatgtgccg agccaccagg ccggcgggaa aatcgagcac     2640

gtaaaccccg aggtctacgc gattttggag cgctgggcac gcctggaaaa agcgccagct     2700

tggatcggcg tgaatccact gagcgggaaa tgccagctca tctggctcat tgatccggtg     2760

tatgccgcag caggcatgag cagcccgaat atgcgcctgc tggctgcaac gaccgaggaa     2820

atgacccgcg ttttcggcgc tgaccaggct ttttcacata ggctgagccg gtggccactg     2880

cacgtctccg acgatcccac cgcgtaccgc tggcatgccc agcacaatcg cgtggatcgc     2940

ctagctgatc ttatggaggt tgctcgcatg atctcaggca cagaaaaacc taaaaaacgc     3000

tatgagcagg agttttctag cggacgggca cgtatcgaag cggcaagaaa agccactgcg     3060

gaagcaaaag cacttgccac gcttgaagca agcctgccga gcgccgctga agcgtctgga     3120

gagctgatcg acggcgtccg tgtcctctgg actgctccag ggcgtgccgc ccgtgatgag     3180

acggcttttc gccacgcttt gactgtggga taccagttaa aagcggctgg tgagcgccta     3240

aaagacacca agatcatcga cgcctacgag cgtgcctaca ccgtcgctca ggcggtcgga     3300

gcagacggcc gtgagcctga tctgccgccg atgcgtgacc gccagacgat ggcgcgacgt     3360

gtgcgcggct acgtcgctaa aggccagcca gtcgtccctg ctcgtcagac agagacgcag     3420

agcagccgag ggcgaaaagc tctggccact atgggaagac gtggcggtaa aaaggccgca     3480

gaacgctgga aagacccaaa cagtgagtac gcccgagcac agcgagaaaa actagctaag     3540

tccagtcaac gacaagctag gaaagctaaa ggaaatcgct tgaccattgc aggttggttt     3600

atgactgttg agggagagac tggctcgtgg cgacaatcaa tgaagctatg tctgaattta     3660

gcgtgtcacg tcagaccgtg aatagagcac ttaagtctgc gggcattgaa cttccacgag     3720

gacgccgtaa agcttcccag taaatgtgcc atctcgtagg cagaaaacgg ttccccccgt     3780

aggggtctct ctcttggcct cctttctagg tcgggctgat tgctcttgaa gctctctagg     3840

ggggctcaca ccataggcag ataacggttc cccaccggct cacctcgtaa gcgcacaagg     3900

actgctccca atgccgcaag cactcagggc gcaagggctg ctaaaggaag cggaacacgt     3960

agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct     4020

ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc     4080

gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc     4140

cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga     4200

tctgatggcg caggggatca agatctgatc aagagacagg atgaggatcg tttcgcatga     4260

ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct     4320

atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc     4380

aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactccaag     4440

acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg     4500

acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc     4560

tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc     4620

ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg     4680

agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc     4740

atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcggatg cccgacggcg     4800

aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc     4860

gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag     4920

cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg     4980

tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg     5040

agttcttctg agcgggactc tggggttcgc tagaggatcg atccttttta acccatcaca     5100

tatacctgcc gttcactatt atttagtgaa atgagatatt atgatatttt ctgaattgtg     5160

attaaaaagg caactttatg cccatgcaac agaaactata aaaaatacag agaatgaaaa     5220

gaaacagata gattttttag ttctttaggc ccgtagtctg caaatccttt tatgattttc     5280

tatcaaacaa aagaggaaaa tagaccagtt gcaatccaaa cgagagtcta acgacgcgag     5340

gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt     5400

tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc     5460

tcgcggctct taccagccta acttcgatca ttggaccgct gatcgtcacg gcgatttatg     5520

ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg     5580

tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag     5640

ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg     5700

gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc     5760

cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg tgcgcatgat     5820

cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa     5880

tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg     5940

agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc     6000

agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg     6060

aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc     6120

ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc     6180

atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat     6240

gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa     6300

catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga     6360

cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg     6420

cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga     6480

gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc     6540

agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt     6600

gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg     6660

tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc     6720

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca     6780

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca     6840

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg     6900

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg     6960

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt     7020

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt     7080

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc     7140

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt     7200

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt     7260

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc     7320

tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa     7380

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt     7440

tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct     7500

acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta     7560

tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa     7620

agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc     7680

tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact     7740

acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc     7800

tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt     7860

ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta     7920

agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg     7980

tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt     8040

acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc     8100

agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt     8160

actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc     8220

tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc     8280

gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa     8340

ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac     8400

tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa     8460

aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt     8520

tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa     8580

tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct     8640

gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg     8700

ccctttcgtc ttcaagaa                                                   8718


<210>  39
<211>  7250
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10439; beta actin hairpin - coat protein


<220>
<221>  promoter
<222>  (187)..(467)
<223>  yeast TRP1 promoter

<220>
<221>  gene
<222>  (468)..(1142)
<223>  yeast TRP1 gene

<220>
<221>  misc_feature
<222>  (2082)..(2087)
<223>  restriction endonuclease SacI recognition site

<220>
<221>  misc_feature
<222>  (2152)..(2157)
<223>  restriction endonuclease EcoRI recognition site

<220>
<221>  promoter
<222>  (2162)..(2826)
<223>  yeast GAL1, 10 bidirectional prmoter

<220>
<221>  misc_feature
<222>  (2844)..(2851)
<223>  restriction endonuclease AsiSI recognition sequence

<220>
<221>  misc_feature
<222>  (2852)..(3146)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (3146)..(3153)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (3311)..(3605)
<223>  beta actin antisense strand gene fragment

<220>
<221>  terminator
<222>  (3657)..(3846)
<223>  yeast CYC1 terminator

<220>
<221>  misc_feature
<222>  (5840)..(7182)
<223>  yeast 2 micron ori

<400>  39
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat      240

atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa      300

aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa      360

gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat      420

tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta      480

atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg      540

cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa      600

agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa      660

atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg      720

ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt      780

ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag      840

actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg      900

attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg      960

gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa     1020

atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg     1080

agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat     1140

aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga     1200

aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt     1260

ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa     1320

atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca     1380

gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc     1440

gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg     1500

aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg     1560

ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg     1620

gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg     1680

ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga     1740

tcggtgcggg cctcttcgct attacgccag ctgaattgga gcgacctcat gctatacctg     1800

agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac     1860

ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa     1920

aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc taccaacgat     1980

ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga caaccttgat     2040

tggagacttg accaaacctc tggcgaagaa ttgttaatta agagctcaga tcttatcgtc     2100

gtcatccttg taatccatcg atactagtgc ggccgccctt tagtgagggt tgaattcgaa     2160

ttttcaaaaa ttcttacttt ttttttggat ggacgcaaag aagtttaata atcatattac     2220

atggcattac caccatatac atatccatat acatatccat atctaatctt acttatatgt     2280

tgtggaaatg taaagagccc cattatctta gcctaaaaaa accttctctt tggaactttc     2340

agtaatacgc ttaactgctc attgctatat tgaagtacgg attagaagcc gccgagcggg     2400

tgacagccct ccgaaggaag actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc     2460

tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag     2520

cttttatggt tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatga     2580

acgaatcaaa ttaacaacca taggatgata atgcgattag ttttttagcc ttatttctgg     2640

ggtaattaat cagcgaagcg atgatttttg atctattaac agatatataa atgcaaaaac     2700

tgcataacca ctttaactaa tactttcaac attttcggtt tgtattactt cttattcaaa     2760

tgtaataaaa gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga     2820

aaaaaccccg gatccattta aatgcgatcg cgcacgaggt ttttctgtct agtgagcagt     2880

gtccaacctc aaaagacaac atgtgtgacg acgatgtagc ggctcttgtc gtagacaatg     2940

gatccggtat gtgcaaagcc ggtttcgcag gagatgacgc accccgtgcc gtcttcccct     3000

cgatcgtcgg tcgcccaagg catcaaggag tcatggtcgg tatgggacaa aaggactcat     3060

acgtaggaga tgaagcccaa agcaaaagag gtatcctcac cctgaaatac cccatcgaac     3120

acggtatcat caccaactgg gatgagttta aaccctctag ctgctttaca aagtactggt     3180

tccctttcca gcgggatgct ttatctaaac gcaatgagag aggtattcct caggccacat     3240

cgcttcctag ttccgctggg atccatcgtt ggcggccgaa gccgccattc catagtgagt     3300

tctggcgcgc ctcatcccag ttggtgatga taccgtgttc gatggggtat ttcagggtga     3360

ggatacctct tttgctttgg gcttcatctc ctacgtatga gtccttttgt cccataccga     3420

ccatgactcc ttgatgcctt gggcgaccga cgatcgaggg gaagacggca cggggtgcgt     3480

catctcctgc gaaaccggct ttgcacatac cggatccatt gtctacgaca agagccgcta     3540

catcgtcgtc acacatgttg tcttttgagg ttggacactg ctcactagac agaaaaacct     3600

cgtgccggac cgaatacccg gtctgaacga gggcggccgc ccgcgggcta gctaagatcc     3660

gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt     3720

atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac     3780

agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc     3840

tcgaagatcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt     3900

gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga     3960

gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca     4020

ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg     4080

ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt     4140

cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc     4200

ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct     4260

tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc     4320

gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta     4380

tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca     4440

gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag     4500

tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag     4560

ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt     4620

agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa     4680

gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg     4740

attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga     4800

agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta     4860

atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc     4920

cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg     4980

ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga     5040

agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt     5100

tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt     5160

gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc     5220

caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc     5280

ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca     5340

gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag     5400

tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg     5460

tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa     5520

cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa     5580

cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga     5640

gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga     5700

atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg     5760

agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt     5820

ccccgaaaag tgccacctga acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa     5880

cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg     5940

caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa     6000

tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag     6060

aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac     6120

aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc     6180

ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt     6240

tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc     6300

ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc     6360

gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg     6420

atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta     6480

cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac     6540

tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag     6600

tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca     6660

gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt     6720

ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc     6780

ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa     6840

taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca     6900

catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca     6960

tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc gtctatttat     7020

gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg     7080

tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt     7140

agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatattaag aaaccattat     7200

tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc                7250


<210>  40
<211>  7582
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE1440; beta actin hairpin + coat protein


<220>
<221>  promoter
<222>  (187)..(467)
<223>  yeast TRP1 promoter

<220>
<221>  gene
<222>  (468)..(1142)
<223>  yeast TRP1 gene

<220>
<221>  misc_feature
<222>  (2082)..(2087)
<223>  restriction endonuclease SacI recognition site

<220>
<221>  gene
<222>  (2091)..(2480)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  misc_feature
<222>  (2484)..(2489)
<223>  restriction endonuclease EcoRI recognition site

<220>
<221>  promoter
<222>  (2494)..(3158)
<223>  yeast GAL1, 10 bidirectional promoter

<220>
<221>  misc_feature
<222>  (3176)..(3183)
<223>  restriction endonuclease AsiSI recognition sequence

<220>
<221>  misc_feature
<222>  (3185)..(3478)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (3478)..(3485)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (3643)..(3937)
<223>  beta actin antisense strand gene fragment

<220>
<221>  terminator
<222>  (3989)..(4178)
<223>  yeast CYC1 terminator

<220>
<221>  misc_feature
<222>  (6172)..(7514)
<223>  yeast 2 micron ori

<400>  40
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat      240

atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa      300

aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa      360

gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat      420

tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta      480

atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg      540

cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa      600

agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa      660

atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg      720

ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt      780

ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag      840

actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg      900

attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg      960

gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa     1020

atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg     1080

agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat     1140

aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga     1200

aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt     1260

ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa     1320

atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca     1380

gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc     1440

gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg     1500

aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg     1560

ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg     1620

gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg     1680

ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga     1740

tcggtgcggg cctcttcgct attacgccag ctgaattgga gcgacctcat gctatacctg     1800

agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac     1860

ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa     1920

aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc taccaacgat     1980

ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga caaccttgat     2040

tggagacttg accaaacctc tggcgaagaa ttgttaatta agagctctta ttagtaaatg     2100

ccagagttag cagcgattgc agatgggatc ggattaccgt ctttcagcag gccctgcatt     2160

gccttaacaa tcagttcgca gtcggagttc gtcgcaaaaa tcggaatagt cagttccatg     2220

ttgaggtaag agcgccaggc cgcaactggg agttctacgc cgcctacggt ctgcgttgcg     2280

actttcggta cttcaacttt gatggtgtat ttgcgattct gcgcgctaga ctgacgaacg     2340

gagcacgtga ctttgtaggc ctgagagcgg ctgttagagc taatccattc cgcaacaccg     2400

ttggcgaagt tggacggagc tacggtaacg tcacccgtac cgccgttatc aaccagaacg     2460

aattgggtaa agttagacgc catgaattcg aattttcaaa aattcttact ttttttttgg     2520

atggacgcaa agaagtttaa taatcatatt acatggcatt accaccatat acatatccat     2580

atacatatcc atatctaatc ttacttatat gttgtggaaa tgtaaagagc cccattatct     2640

tagcctaaaa aaaccttctc tttggaactt tcagtaatac gcttaactgc tcattgctat     2700

attgaagtac ggattagaag ccgccgagcg ggtgacagcc ctccgaagga agactctcct     2760

ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac     2820

tgctccgaac aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg     2880

gcagtaacct ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga     2940

taatgcgatt agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt     3000

tgatctatta acagatatat aaatgcaaaa actgcataac cactttaact aatactttca     3060

acattttcgg tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt     3120

taatatacct ctatacttta acgtcaagga gaaaaaaccc cggatccatt taaatgcgat     3180

cgcgcacgag gtttttctgt ctagtgagca gtgtccaacc tcaaaagaca acatgtgtga     3240

cgacgatgta gcggctcttg tcgtagacaa tggatccggt atgtgcaaag ccggtttcgc     3300

aggagatgac gcaccccgtg ccgtcttccc ctcgatcgtc ggtcgcccaa ggcatcaagg     3360

agtcatggtc ggtatgggac aaaaggactc atacgtagga gatgaagccc aaagcaaaag     3420

aggtatcctc accctgaaat accccatcga acacggtatc atcaccaact gggatgagtt     3480

taaaccctct agctgcttta caaagtactg gttccctttc cagcgggatg ctttatctaa     3540

acgcaatgag agaggtattc ctcaggccac atcgcttcct agttccgctg ggatccatcg     3600

ttggcggccg aagccgccat tccatagtga gttctggcgc gcctcatccc agttggtgat     3660

gataccgtgt tcgatggggt atttcagggt gaggatacct cttttgcttt gggcttcatc     3720

tcctacgtat gagtcctttt gtcccatacc gaccatgact ccttgatgcc ttgggcgacc     3780

gacgatcgag gggaagacgg cacggggtgc gtcatctcct gcgaaaccgg ctttgcacat     3840

accggatcca ttgtctacga caagagccgc tacatcgtcg tcacacatgt tgtcttttga     3900

ggttggacac tgctcactag acagaaaaac ctcgtgccgg accgaatacc cggtctgaac     3960

gagggcggcc gcccgcgggc tagctaagat ccgctctaac cgaaaaggaa ggagttagac     4020

aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat     4080

ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac     4140

tgaaaacctt gcttgagaag gttttgggac gctcgaagat ccagctgcat taatgaatcg     4200

gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg     4260

actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa     4320

tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc     4380

aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc     4440

ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat     4500

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc     4560

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct     4620

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg     4680

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc     4740

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga     4800

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa     4860

ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta     4920

gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc     4980

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg     5040

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga     5100

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg     5160

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct     5220

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg     5280

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc     5340

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa     5400

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc     5460

cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt     5520

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc     5580

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt     5640

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc     5700

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt     5760

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata     5820

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga     5880

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag     5940

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa     6000

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt     6060

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga     6120

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaacgaagca     6180

tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag     6240

aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga     6300

agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa     6360

caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc     6420

aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt     6480

ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt     6540

gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt     6600

tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg     6660

cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc     6720

gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg     6780

aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat     6840

tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac     6900

tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg     6960

tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag atacttttga     7020

gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt     7080

tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt     7140

cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg     7200

agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca cgtcgcacct     7260

atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt gcgtgtttat     7320

gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc tagtacctcc     7380

tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact accctttagc     7440

tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc tatcatttcc     7500

tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta taaaaatagg     7560

cgtatcacga ggccctttcg tc                                              7582


<210>  41
<211>  7269
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10441; beta actin stem loop - coat 
       protein


<220>
<221>  promoter
<222>  (187)..(467)
<223>  yeast TRP1 promoter

<220>
<221>  gene
<222>  (468)..(1142)
<223>  yeast TRP1 gene

<220>
<221>  misc_feature
<222>  (2082)..(2087)
<223>  restriction endonuclease SacI recognition site

<220>
<221>  misc_feature
<222>  (2152)..(2157)
<223>  restriction endonuclease EcoRI recognition site

<220>
<221>  promoter
<222>  (2162)..(2826)
<223>  yeast GAL1, 10 bidirectional promoter

<220>
<221>  misc_feature
<222>  (2844)..(2851)
<223>  restriction endonuclease AsiSI recognition sequence

<220>
<221>  misc_feature
<222>  (2852)..(3146)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (3146)..(3153)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (3311)..(3605)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (3641)..(3659)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (3676)..(3865)
<223>  yeast CYC1 terminator

<220>
<221>  misc_feature
<222>  (5859)..(7201)
<223>  yeast 2 micron ori

<400>  41
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat      240

atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa      300

aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa      360

gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat      420

tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta      480

atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg      540

cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa      600

agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa      660

atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg      720

ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt      780

ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag      840

actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg      900

attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg      960

gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa     1020

atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg     1080

agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat     1140

aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga     1200

aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt     1260

ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa     1320

atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca     1380

gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc     1440

gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg     1500

aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg     1560

ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg     1620

gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg     1680

ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga     1740

tcggtgcggg cctcttcgct attacgccag ctgaattgga gcgacctcat gctatacctg     1800

agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac     1860

ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa     1920

aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc taccaacgat     1980

ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga caaccttgat     2040

tggagacttg accaaacctc tggcgaagaa ttgttaatta agagctcaga tcttatcgtc     2100

gtcatccttg taatccatcg atactagtgc ggccgccctt tagtgagggt tgaattcgaa     2160

ttttcaaaaa ttcttacttt ttttttggat ggacgcaaag aagtttaata atcatattac     2220

atggcattac caccatatac atatccatat acatatccat atctaatctt acttatatgt     2280

tgtggaaatg taaagagccc cattatctta gcctaaaaaa accttctctt tggaactttc     2340

agtaatacgc ttaactgctc attgctatat tgaagtacgg attagaagcc gccgagcggg     2400

tgacagccct ccgaaggaag actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc     2460

tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag     2520

cttttatggt tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatga     2580

acgaatcaaa ttaacaacca taggatgata atgcgattag ttttttagcc ttatttctgg     2640

ggtaattaat cagcgaagcg atgatttttg atctattaac agatatataa atgcaaaaac     2700

tgcataacca ctttaactaa tactttcaac attttcggtt tgtattactt cttattcaaa     2760

tgtaataaaa gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga     2820

aaaaaccccg gatccattta aatgcgatcg cgcacgaggt ttttctgtct agtgagcagt     2880

gtccaacctc aaaagacaac atgtgtgacg acgatgtagc ggctcttgtc gtagacaatg     2940

gatccggtat gtgcaaagcc ggtttcgcag gagatgacgc accccgtgcc gtcttcccct     3000

cgatcgtcgg tcgcccaagg catcaaggag tcatggtcgg tatgggacaa aaggactcat     3060

acgtaggaga tgaagcccaa agcaaaagag gtatcctcac cctgaaatac cccatcgaac     3120

acggtatcat caccaactgg gatgagttta aaccctctag ctgctttaca aagtactggt     3180

tccctttcca gcgggatgct ttatctaaac gcaatgagag aggtattcct caggccacat     3240

cgcttcctag ttccgctggg atccatcgtt ggcggccgaa gccgccattc catagtgagt     3300

tctggcgcgc ctcatcccag ttggtgatga taccgtgttc gatggggtat ttcagggtga     3360

ggatacctct tttgctttgg gcttcatctc ctacgtatga gtccttttgt cccataccga     3420

ccatgactcc ttgatgcctt gggcgaccga cgatcgaggg gaagacggca cggggtgcgt     3480

catctcctgc gaaaccggct ttgcacatac cggatccatt gtctacgaca agagccgcta     3540

catcgtcgtc acacatgttg tcttttgagg ttggacactg ctcactagac agaaaaacct     3600

cgtgccggac cgaatacccg gtctgaacga gggcggccgc acatgaggat cacccatgtc     3660

cgcgggctag ctaagatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta     3720

ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat     3780

ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct     3840

tgagaaggtt ttgggacgct cgaagatcca gctgcattaa tgaatcggcc aacgcgcggg     3900

gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc     3960

ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac     4020

agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa     4080

ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca     4140

caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc     4200

gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata     4260

cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta     4320

tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca     4380

gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga     4440

cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg     4500

tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg     4560

tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg     4620

caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag     4680

aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa     4740

cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat     4800

ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc     4860

tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc     4920

atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc     4980

tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc     5040

aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc     5100

catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt     5160

gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc     5220

ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa     5280

aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt     5340

atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg     5400

cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc     5460

gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa     5520

agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt     5580

gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt     5640

caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag     5700

ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta     5760

tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat     5820

aggggttccg cgcacatttc cccgaaaagt gccacctgaa cgaagcatct gtgcttcatt     5880

ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca     5940

tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt     6000

catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag     6060

ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta     6120

tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc     6180

ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc     6240

actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa     6300

aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt     6360

tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga     6420

acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct     6480

attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca     6540

ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat     6600

aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt     6660

tatataggga tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg     6720

aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga     6780

aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta     6840

gagaatagga acttcggaat aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa     6900

atgcaacgcg agctgcgcac atacagctca ctgttcacgt cgcacctata tctgcgtgtt     6960

gcctgtatat atatatacat gagaagaacg gcatagtgcg tgtttatgct taaatgcgta     7020

cttatatgcg tctatttatg taggatgaaa ggtagtctag tacctcctgt gatattatcc     7080

cattccatgc ggggtatcgt atgcttcctt cagcactacc ctttagctgt tctatatgct     7140

gccactcctc aattggatta gtctcatcct tcaatgctat catttccttt gatattggat     7200

catattaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc     7260

cctttcgtc                                                             7269


<210>  42
<211>  7601
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10442; beta actin hairpin + coat protein


<220>
<221>  promoter
<222>  (187)..(467)
<223>  yeast TRP1 promoter

<220>
<221>  gene
<222>  (468)..(1142)
<223>  yeast TRP1 gene

<220>
<221>  misc_feature
<222>  (2082)..(2087)
<223>  restriction endonuclease SacI recognition site

<220>
<221>  gene
<222>  (2091)..(2480)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  misc_feature
<222>  (2484)..(2489)
<223>  restriction endonuclease EcoRI recognition site

<220>
<221>  promoter
<222>  (2494)..(3158)
<223>  yeast GAL1, 10 bidirectional promoter

<220>
<221>  misc_feature
<222>  (3176)..(3183)
<223>  restriction endonuclease AsiSI recognition sequence

<220>
<221>  misc_feature
<222>  (3185)..(3478)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (3478)..(3485)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (3643)..(3937)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (3973)..(3991)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (4008)..(4197)
<223>  yeast CYC1 terminator

<220>
<221>  misc_feature
<222>  (6191)..(7533)
<223>  yeast 2 micron ori

<400>  42
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat      240

atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa      300

aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa      360

gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat      420

tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta      480

atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg      540

cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa      600

agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa      660

atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg      720

ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt      780

ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag      840

actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg      900

attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg      960

gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa     1020

atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg     1080

agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat     1140

aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga     1200

aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt     1260

ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa     1320

atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca     1380

gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc     1440

gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg     1500

aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg     1560

ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg     1620

gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg     1680

ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga     1740

tcggtgcggg cctcttcgct attacgccag ctgaattgga gcgacctcat gctatacctg     1800

agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac     1860

ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa     1920

aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc taccaacgat     1980

ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga caaccttgat     2040

tggagacttg accaaacctc tggcgaagaa ttgttaatta agagctctta ttagtaaatg     2100

ccagagttag cagcgattgc agatgggatc ggattaccgt ctttcagcag gccctgcatt     2160

gccttaacaa tcagttcgca gtcggagttc gtcgcaaaaa tcggaatagt cagttccatg     2220

ttgaggtaag agcgccaggc cgcaactggg agttctacgc cgcctacggt ctgcgttgcg     2280

actttcggta cttcaacttt gatggtgtat ttgcgattct gcgcgctaga ctgacgaacg     2340

gagcacgtga ctttgtaggc ctgagagcgg ctgttagagc taatccattc cgcaacaccg     2400

ttggcgaagt tggacggagc tacggtaacg tcacccgtac cgccgttatc aaccagaacg     2460

aattgggtaa agttagacgc catgaattcg aattttcaaa aattcttact ttttttttgg     2520

atggacgcaa agaagtttaa taatcatatt acatggcatt accaccatat acatatccat     2580

atacatatcc atatctaatc ttacttatat gttgtggaaa tgtaaagagc cccattatct     2640

tagcctaaaa aaaccttctc tttggaactt tcagtaatac gcttaactgc tcattgctat     2700

attgaagtac ggattagaag ccgccgagcg ggtgacagcc ctccgaagga agactctcct     2760

ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac     2820

tgctccgaac aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg     2880

gcagtaacct ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga     2940

taatgcgatt agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt     3000

tgatctatta acagatatat aaatgcaaaa actgcataac cactttaact aatactttca     3060

acattttcgg tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt     3120

taatatacct ctatacttta acgtcaagga gaaaaaaccc cggatccatt taaatgcgat     3180

cgcgcacgag gtttttctgt ctagtgagca gtgtccaacc tcaaaagaca acatgtgtga     3240

cgacgatgta gcggctcttg tcgtagacaa tggatccggt atgtgcaaag ccggtttcgc     3300

aggagatgac gcaccccgtg ccgtcttccc ctcgatcgtc ggtcgcccaa ggcatcaagg     3360

agtcatggtc ggtatgggac aaaaggactc atacgtagga gatgaagccc aaagcaaaag     3420

aggtatcctc accctgaaat accccatcga acacggtatc atcaccaact gggatgagtt     3480

taaaccctct agctgcttta caaagtactg gttccctttc cagcgggatg ctttatctaa     3540

acgcaatgag agaggtattc ctcaggccac atcgcttcct agttccgctg ggatccatcg     3600

ttggcggccg aagccgccat tccatagtga gttctggcgc gcctcatccc agttggtgat     3660

gataccgtgt tcgatggggt atttcagggt gaggatacct cttttgcttt gggcttcatc     3720

tcctacgtat gagtcctttt gtcccatacc gaccatgact ccttgatgcc ttgggcgacc     3780

gacgatcgag gggaagacgg cacggggtgc gtcatctcct gcgaaaccgg ctttgcacat     3840

accggatcca ttgtctacga caagagccgc tacatcgtcg tcacacatgt tgtcttttga     3900

ggttggacac tgctcactag acagaaaaac ctcgtgccgg accgaatacc cggtctgaac     3960

gagggcggcc gcacatgagg atcacccatg tccgcgggct agctaagatc cgctctaacc     4020

gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg     4080

ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg     4140

tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaagatc     4200

cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct     4260

tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca     4320

gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac     4380

atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt     4440

ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg     4500

cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc     4560

tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc     4620

gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc     4680

aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac     4740

tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt     4800

aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct     4860

aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc     4920

ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt     4980

ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg     5040

atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc     5100

atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa     5160

tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag     5220

gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg     5280

tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga     5340

gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag     5400

cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa     5460

gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc     5520

atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca     5580

aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg     5640

atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat     5700

aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc     5760

aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg     5820

gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg     5880

gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt     5940

gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca     6000

ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata     6060

ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac     6120

atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa     6180

gtgccacctg aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc     6240

gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa     6300

agcgctattt taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg     6360

agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac     6420

gcgagagcgc tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca     6480

tcccgagagc gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg     6540

ctctataatg cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag     6600

gctactttgg tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta     6660

ctgattacta gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt     6720

ctataccgat gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt     6780

cattggtcag aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga     6840

aatgtttaca ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt     6900

tttgtctaaa gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc     6960

aagttcaagg agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat     7020

agcaaagaga tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct     7080

cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt     7140

tcaaaagcgc tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt     7200

caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct     7260

cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa     7320

cggcatagtg cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga     7380

aaggtagtct agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc     7440

ttcagcacta ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc     7500

cttcaatgct atcatttcct ttgatattgg atcatattaa gaaaccatta ttatcatgac     7560

attaacctat aaaaataggc gtatcacgag gccctttcgt c                         7601


<210>  43
<211>  7288
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10443; beta actin stem loop - coat 
       protein


<220>
<221>  promoter
<222>  (187)..(467)
<223>  yeast TRP1 promoter

<220>
<221>  gene
<222>  (468)..(1142)
<223>  yeast TRP1 gene

<220>
<221>  misc_feature
<222>  (2082)..(2087)
<223>  restriction endonuclease SacI recognition site

<220>
<221>  misc_feature
<222>  (2152)..(2157)
<223>  restriction endonuclease EcoRI recognition site

<220>
<221>  promoter
<222>  (2162)..(2826)
<223>  yeast GAL1, 10 bidirectional promoter

<220>
<221>  misc_feature
<222>  (2844)..(2862)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (2863)..(2870)
<223>  restriction endonuclease AsiSI recognition sequence

<220>
<221>  misc_feature
<222>  (2872)..(3165)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (3165)..(3172)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (3329)..(3624)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (3660)..(3678)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (3695)..(3884)
<223>  yeast CYC1 terminator

<220>
<221>  misc_feature
<222>  (5878)..(7220)
<223>  yeast 2 micron ori

<400>  43
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat      240

atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa      300

aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa      360

gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat      420

tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta      480

atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg      540

cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa      600

agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa      660

atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg      720

ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt      780

ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag      840

actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg      900

attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg      960

gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa     1020

atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg     1080

agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat     1140

aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga     1200

aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt     1260

ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa     1320

atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca     1380

gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc     1440

gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg     1500

aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg     1560

ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg     1620

gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg     1680

ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga     1740

tcggtgcggg cctcttcgct attacgccag ctgaattgga gcgacctcat gctatacctg     1800

agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac     1860

ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa     1920

aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc taccaacgat     1980

ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga caaccttgat     2040

tggagacttg accaaacctc tggcgaagaa ttgttaatta agagctcaga tcttatcgtc     2100

gtcatccttg taatccatcg atactagtgc ggccgccctt tagtgagggt tgaattcgaa     2160

ttttcaaaaa ttcttacttt ttttttggat ggacgcaaag aagtttaata atcatattac     2220

atggcattac caccatatac atatccatat acatatccat atctaatctt acttatatgt     2280

tgtggaaatg taaagagccc cattatctta gcctaaaaaa accttctctt tggaactttc     2340

agtaatacgc ttaactgctc attgctatat tgaagtacgg attagaagcc gccgagcggg     2400

tgacagccct ccgaaggaag actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc     2460

tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag     2520

cttttatggt tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatga     2580

acgaatcaaa ttaacaacca taggatgata atgcgattag ttttttagcc ttatttctgg     2640

ggtaattaat cagcgaagcg atgatttttg atctattaac agatatataa atgcaaaaac     2700

tgcataacca ctttaactaa tactttcaac attttcggtt tgtattactt cttattcaaa     2760

tgtaataaaa gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga     2820

aaaaaccccg gatccattta aatacatgag gattacccat gtgcgatcgc gcacgaggtt     2880

tttctgtcta gtgagcagtg tccaacctca aaagacaaca tgtgtgacga cgatgtagcg     2940

gctcttgtcg tagacaatgg atccggtatg tgcaaagccg gtttcgcagg agatgacgca     3000

ccccgtgccg tcttcccctc gatcgtcggt cgcccaaggc atcaaggagt catggtcggt     3060

atgggacaaa aggactcata cgtaggagat gaagcccaaa gcaaaagagg tatcctcacc     3120

ctgaaatacc ccatcgaaca cggtatcatc accaactggg atgagtttaa accctctagc     3180

tgctttacaa agtactggtt ccctttccag cgggatgctt tatctaaacg caatgagaga     3240

ggtattcctc aggccacatc gcttcctagt tccgctggga tccatcgttg gcggccgaag     3300

ccgccattcc atagtgagtt ctggcgcgcc tcatcccagt tggtgatgat accgtgttcg     3360

atggggtatt tcagggtgag gatacctctt ttgctttggg cttcatctcc tacgtatgag     3420

tccttttgtc ccataccgac catgactcct tgatgccttg ggcgaccgac gatcgagggg     3480

aagacggcac ggggtgcgtc atctcctgcg aaaccggctt tgcacatacc ggatccattg     3540

tctacgacaa gagccgctac atcgtcgtca cacatgttgt cttttgaggt tggacactgc     3600

tcactagaca gaaaaacctc gtgccggacc gaatacccgg tctgaacgag ggcggccgca     3660

catgaggatc acccatgtcc gcgggctagc taagatccgc tctaaccgaa aaggaaggag     3720

ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa     3780

cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca     3840

ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaagatccag ctgcattaat     3900

gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc     3960

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg     4020

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag     4080

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc     4140

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag     4200

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga     4260

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc     4320

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg     4380

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt     4440

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca     4500

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca     4560

ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag     4620

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca     4680

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg     4740

ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa     4800

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta     4860

tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag     4920

cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga     4980

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac     5040

cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc     5100

ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta     5160

gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac     5220

gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat     5280

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa     5340

gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg     5400

tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag     5460

aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc     5520

cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct     5580

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat     5640

cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg     5700

ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc     5760

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta     5820

tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac     5880

gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa     5940

acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac     6000

caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt     6060

ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat     6120

tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct     6180

atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag     6240

tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt     6300

ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg     6360

aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg     6420

gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa     6480

attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt     6540

tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag     6600

taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc     6660

gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac     6720

ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg     6780

tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct     6840

gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg     6900

aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc     6960

gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt     7020

gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt     7080

acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc     7140

tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc     7200

atttcctttg atattggatc atattaagaa accattatta tcatgacatt aacctataaa     7260

aataggcgta tcacgaggcc ctttcgtc                                        7288


<210>  44
<211>  7620
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10444; beta actin stem loop + coat 
       protein


<220>
<221>  promoter
<222>  (187)..(467)
<223>  yeast TRP1 promoter

<220>
<221>  gene
<222>  (468)..(1142)
<223>  yeast TRP1 gene

<220>
<221>  misc_feature
<222>  (2082)..(2087)
<223>  restriction endonuclease SacI recognition site

<220>
<221>  gene
<222>  (2091)..(2480)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  misc_feature
<222>  (2484)..(2489)
<223>  restriction endonuclease EcoRI recognition site

<220>
<221>  promoter
<222>  (2494)..(3158)
<223>  yeast GAL1, 10 bidirectional promoter

<220>
<221>  misc_feature
<222>  (3176)..(3194)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (3195)..(3202)
<223>  restriction endonuclease AsiSI recognition sequence

<220>
<221>  misc_feature
<222>  (3204)..(3497)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (3497)..(3504)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (3661)..(3957)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (3992)..(4010)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (4027)..(4216)
<223>  yeast CYC1 terminator

<220>
<221>  misc_feature
<222>  (6210)..(7552)
<223>  yeast 2 micron ori

<400>  44
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat      240

atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa      300

aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa      360

gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat      420

tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta      480

atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg      540

cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa      600

agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa      660

atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg      720

ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt      780

ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag      840

actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg      900

attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg      960

gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa     1020

atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg     1080

agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat     1140

aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga     1200

aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt     1260

ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa     1320

atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca     1380

gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc     1440

gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg     1500

aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg     1560

ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg     1620

gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg     1680

ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga     1740

tcggtgcggg cctcttcgct attacgccag ctgaattgga gcgacctcat gctatacctg     1800

agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac     1860

ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa     1920

aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc taccaacgat     1980

ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga caaccttgat     2040

tggagacttg accaaacctc tggcgaagaa ttgttaatta agagctctta ttagtaaatg     2100

ccagagttag cagcgattgc agatgggatc ggattaccgt ctttcagcag gccctgcatt     2160

gccttaacaa tcagttcgca gtcggagttc gtcgcaaaaa tcggaatagt cagttccatg     2220

ttgaggtaag agcgccaggc cgcaactggg agttctacgc cgcctacggt ctgcgttgcg     2280

actttcggta cttcaacttt gatggtgtat ttgcgattct gcgcgctaga ctgacgaacg     2340

gagcacgtga ctttgtaggc ctgagagcgg ctgttagagc taatccattc cgcaacaccg     2400

ttggcgaagt tggacggagc tacggtaacg tcacccgtac cgccgttatc aaccagaacg     2460

aattgggtaa agttagacgc catgaattcg aattttcaaa aattcttact ttttttttgg     2520

atggacgcaa agaagtttaa taatcatatt acatggcatt accaccatat acatatccat     2580

atacatatcc atatctaatc ttacttatat gttgtggaaa tgtaaagagc cccattatct     2640

tagcctaaaa aaaccttctc tttggaactt tcagtaatac gcttaactgc tcattgctat     2700

attgaagtac ggattagaag ccgccgagcg ggtgacagcc ctccgaagga agactctcct     2760

ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac     2820

tgctccgaac aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg     2880

gcagtaacct ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga     2940

taatgcgatt agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt     3000

tgatctatta acagatatat aaatgcaaaa actgcataac cactttaact aatactttca     3060

acattttcgg tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt     3120

taatatacct ctatacttta acgtcaagga gaaaaaaccc cggatccatt taaatacatg     3180

aggattaccc atgtgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct     3240

caaaagacaa catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta     3300

tgtgcaaagc cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg     3360

gtcgcccaag gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag     3420

atgaagccca aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca     3480

tcaccaactg ggatgagttt aaaccctcta gctgctttac aaagtactgg ttccctttcc     3540

agcgggatgc tttatctaaa cgcaatgaga gaggtattcc tcaggccaca tcgcttccta     3600

gttccgctgg gatccatcgt tggcggccga agccgccatt ccatagtgag ttctggcgcg     3660

cctcatccca gttggtgatg ataccgtgtt cgatggggta tttcagggtg aggatacctc     3720

ttttgctttg ggcttcatct cctacgtatg agtccttttg tcccataccg accatgactc     3780

cttgatgcct tgggcgaccg acgatcgagg ggaagacggc acggggtgcg tcatctcctg     3840

cgaaaccggc tttgcacata ccggatccat tgtctacgac aagagccgct acatcgtcgt     3900

cacacatgtt gtcttttgag gttggacact gctcactaga cagaaaaacc tcgtgccgga     3960

ccgaataccc ggtctgaacg agggcggccg cacatgagga tcacccatgt ccgcgggcta     4020

gctaagatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat     4080

ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt     4140

ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt     4200

tttgggacgc tcgaagatcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg     4260

tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg     4320

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg     4380

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa     4440

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg     4500

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc     4560

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc     4620

ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc     4680

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg     4740

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc     4800

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga     4860

gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc     4920

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac     4980

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg     5040

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc     5100

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa     5160

ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta     5220

ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt     5280

tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag     5340

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca     5400

gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc     5460

tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt     5520

tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag     5580

ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt     5640

tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat     5700

ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt     5760

gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc     5820

ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat     5880

cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag     5940

ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt     6000

ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg     6060

gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta     6120

ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc     6180

gcgcacattt ccccgaaaag tgccacctga acgaagcatc tgtgcttcat tttgtagaac     6240

aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag     6300

aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt     6360

aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt     6420

tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt     6480

tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac     6540

tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt     6600

ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg     6660

actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa     6720

aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt     6780

gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct     6840

ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa     6900

tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt     6960

agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg     7020

atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat     7080

tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc     7140

ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg     7200

aacttcggaa taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc     7260

gagctgcgca catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata     7320

tatatataca tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc     7380

gtctatttat gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg     7440

cggggtatcg tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct     7500

caattggatt agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatattaag     7560

aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc     7620


<210>  45
<211>  7288
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recpmbinant plasmid pAPSE10445; beta actin stem loop - coat 
       protein


<220>
<221>  promoter
<222>  (187)..(467)
<223>  yeast TRP1 promoter

<220>
<221>  gene
<222>  (468)..(1142)
<223>  yeast TRP1 gene

<220>
<221>  misc_feature
<222>  (2082)..(2087)
<223>  restriction endonuclease SacI recognition site

<220>
<221>  misc_feature
<222>  (2152)..(2157)
<223>  restriction endonuclease EcoRI recognition site

<220>
<221>  misc_feature
<222>  (2162)..(2826)
<223>  yeast GAL1, 10 bidirectional prmoter

<220>
<221>  misc_feature
<222>  (2844)..(2862)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (2863)..(2870)
<223>  restriction endonuclease AsiSI recognition sequence

<220>
<221>  misc_feature
<222>  (2872)..(3165)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (3165)..(3172)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (3233)..(3251)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (3329)..(3624)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (3660)..(3678)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (3695)..(3884)
<223>  yeast CYC1 terminator

<220>
<221>  misc_feature
<222>  (5878)..(7220)
<223>  yeast 2 micron ori

<400>  45
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat      240

atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa      300

aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa      360

gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat      420

tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta      480

atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg      540

cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa      600

agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa      660

atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg      720

ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt      780

ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag      840

actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg      900

attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg      960

gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa     1020

atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg     1080

agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat     1140

aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga     1200

aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt     1260

ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa     1320

atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca     1380

gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc     1440

gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg     1500

aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg     1560

ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg     1620

gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg     1680

ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga     1740

tcggtgcggg cctcttcgct attacgccag ctgaattgga gcgacctcat gctatacctg     1800

agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac     1860

ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa     1920

aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc taccaacgat     1980

ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga caaccttgat     2040

tggagacttg accaaacctc tggcgaagaa ttgttaatta agagctcaga tcttatcgtc     2100

gtcatccttg taatccatcg atactagtgc ggccgccctt tagtgagggt tgaattcgaa     2160

ttttcaaaaa ttcttacttt ttttttggat ggacgcaaag aagtttaata atcatattac     2220

atggcattac caccatatac atatccatat acatatccat atctaatctt acttatatgt     2280

tgtggaaatg taaagagccc cattatctta gcctaaaaaa accttctctt tggaactttc     2340

agtaatacgc ttaactgctc attgctatat tgaagtacgg attagaagcc gccgagcggg     2400

tgacagccct ccgaaggaag actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc     2460

tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag     2520

cttttatggt tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatga     2580

acgaatcaaa ttaacaacca taggatgata atgcgattag ttttttagcc ttatttctgg     2640

ggtaattaat cagcgaagcg atgatttttg atctattaac agatatataa atgcaaaaac     2700

tgcataacca ctttaactaa tactttcaac attttcggtt tgtattactt cttattcaaa     2760

tgtaataaaa gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga     2820

aaaaaccccg gatccattta aatacatgag gattacccat gtgcgatcgc gcacgaggtt     2880

tttctgtcta gtgagcagtg tccaacctca aaagacaaca tgtgtgacga cgatgtagcg     2940

gctcttgtcg tagacaatgg atccggtatg tgcaaagccg gtttcgcagg agatgacgca     3000

ccccgtgccg tcttcccctc gatcgtcggt cgcccaaggc atcaaggagt catggtcggt     3060

atgggacaaa aggactcata cgtaggagat gaagcccaaa gcaaaagagg tatcctcacc     3120

ctgaaatacc ccatcgaaca cggtatcatc accaactggg atgagtttaa accctctagc     3180

tgctttacaa agtactggtt ccctttccag cgggatgctt tatctaaacg caacatgagg     3240

atcacccatg tcgccacatc gcttcctagt tccgctggga tccatcgttg gcggccgaag     3300

ccgccattcc atagtgagtt ctggcgcgcc tcatcccagt tggtgatgat accgtgttcg     3360

atggggtatt tcagggtgag gatacctctt ttgctttggg cttcatctcc tacgtatgag     3420

tccttttgtc ccataccgac catgactcct tgatgccttg ggcgaccgac gatcgagggg     3480

aagacggcac ggggtgcgtc atctcctgcg aaaccggctt tgcacatacc ggatccattg     3540

tctacgacaa gagccgctac atcgtcgtca cacatgttgt cttttgaggt tggacactgc     3600

tcactagaca gaaaaacctc gtgccggacc gaatacccgg tctgaacgag ggcggccgca     3660

catgaggatc acccatgtcc gcgggctagc taagatccgc tctaaccgaa aaggaaggag     3720

ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa     3780

cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca     3840

ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaagatccag ctgcattaat     3900

gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc     3960

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg     4020

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag     4080

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc     4140

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag     4200

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga     4260

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc     4320

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg     4380

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt     4440

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca     4500

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca     4560

ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag     4620

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca     4680

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg     4740

ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa     4800

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta     4860

tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag     4920

cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga     4980

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac     5040

cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc     5100

ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta     5160

gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac     5220

gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat     5280

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa     5340

gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg     5400

tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag     5460

aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc     5520

cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct     5580

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat     5640

cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg     5700

ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc     5760

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta     5820

tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac     5880

gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa     5940

acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac     6000

caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt     6060

ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat     6120

tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct     6180

atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag     6240

tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt     6300

ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg     6360

aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg     6420

gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa     6480

attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt     6540

tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag     6600

taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc     6660

gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac     6720

ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg     6780

tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct     6840

gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg     6900

aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc     6960

gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt     7020

gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt     7080

acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc     7140

tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc     7200

atttcctttg atattggatc atattaagaa accattatta tcatgacatt aacctataaa     7260

aataggcgta tcacgaggcc ctttcgtc                                        7288


<210>  46
<211>  7620
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant plasmid pAPSE10446; beta actin stem loop + coat 
       protein


<220>
<221>  promoter
<222>  (187)..(467)
<223>  yeast TRP1 promoter

<220>
<221>  gene
<222>  (468)..(1142)
<223>  yeast TRP1 gene

<220>
<221>  misc_feature
<222>  (2082)..(2087)
<223>  restriction endonuclease SacI recognition site

<220>
<221>  gene
<222>  (2091)..(2480)
<223>  bacteriophage MS2 coat protein gene

<220>
<221>  misc_feature
<222>  (2484)..(2489)
<223>  restriction endonuclease EcoRI recognition site

<220>
<221>  promoter
<222>  (2494)..(3158)
<223>  yeast GAL1, 10 bidirectional promoter

<220>
<221>  misc_feature
<222>  (3176)..(3194)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (3195)..(3202)
<223>  restriction endonuclease AsiSI recognition sequence

<220>
<221>  misc_feature
<222>  (3204)..(3497)
<223>  beta actin sense gene fragment

<220>
<221>  misc_feature
<222>  (3497)..(3504)
<223>  restriction endonuclease PmeI recognition site

<220>
<221>  misc_feature
<222>  (3565)..(3583)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  misc_feature
<222>  (3662)..(3956)
<223>  beta actin antisense strand gene fragment

<220>
<221>  misc_feature
<222>  (3992)..(4010)
<223>  bacteriophage MS2 pac sequence

<220>
<221>  terminator
<222>  (4027)..(4216)
<223>  yeast CYC1 terminator

<220>
<221>  misc_feature
<222>  (6210)..(7552)
<223>  yeast 2 micron ori

<400>  46
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat      240

atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa      300

aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa      360

gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat      420

tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta      480

atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg      540

cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa      600

agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa      660

atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg      720

ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt      780

ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag      840

actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg      900

attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg      960

gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa     1020

atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg     1080

agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat     1140

aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga     1200

aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt     1260

ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa     1320

atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca     1380

gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc     1440

gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg     1500

aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg     1560

ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg     1620

gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg     1680

ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga     1740

tcggtgcggg cctcttcgct attacgccag ctgaattgga gcgacctcat gctatacctg     1800

agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac     1860

ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa     1920

aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc taccaacgat     1980

ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga caaccttgat     2040

tggagacttg accaaacctc tggcgaagaa ttgttaatta agagctctta ttagtaaatg     2100

ccagagttag cagcgattgc agatgggatc ggattaccgt ctttcagcag gccctgcatt     2160

gccttaacaa tcagttcgca gtcggagttc gtcgcaaaaa tcggaatagt cagttccatg     2220

ttgaggtaag agcgccaggc cgcaactggg agttctacgc cgcctacggt ctgcgttgcg     2280

actttcggta cttcaacttt gatggtgtat ttgcgattct gcgcgctaga ctgacgaacg     2340

gagcacgtga ctttgtaggc ctgagagcgg ctgttagagc taatccattc cgcaacaccg     2400

ttggcgaagt tggacggagc tacggtaacg tcacccgtac cgccgttatc aaccagaacg     2460

aattgggtaa agttagacgc catgaattcg aattttcaaa aattcttact ttttttttgg     2520

atggacgcaa agaagtttaa taatcatatt acatggcatt accaccatat acatatccat     2580

atacatatcc atatctaatc ttacttatat gttgtggaaa tgtaaagagc cccattatct     2640

tagcctaaaa aaaccttctc tttggaactt tcagtaatac gcttaactgc tcattgctat     2700

attgaagtac ggattagaag ccgccgagcg ggtgacagcc ctccgaagga agactctcct     2760

ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac     2820

tgctccgaac aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg     2880

gcagtaacct ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga     2940

taatgcgatt agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt     3000

tgatctatta acagatatat aaatgcaaaa actgcataac cactttaact aatactttca     3060

acattttcgg tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt     3120

taatatacct ctatacttta acgtcaagga gaaaaaaccc cggatccatt taaatacatg     3180

aggattaccc atgtgcgatc gcgcacgagg tttttctgtc tagtgagcag tgtccaacct     3240

caaaagacaa catgtgtgac gacgatgtag cggctcttgt cgtagacaat ggatccggta     3300

tgtgcaaagc cggtttcgca ggagatgacg caccccgtgc cgtcttcccc tcgatcgtcg     3360

gtcgcccaag gcatcaagga gtcatggtcg gtatgggaca aaaggactca tacgtaggag     3420

atgaagccca aagcaaaaga ggtatcctca ccctgaaata ccccatcgaa cacggtatca     3480

tcaccaactg ggatgagttt aaaccctcta gctgctttac aaagtactgg ttccctttcc     3540

agcgggatgc tttatctaaa cgcaacatga ggatcaccca tgtcgccaca tcgcttccta     3600

gttccgctgg gatccatcgt tggcggccga agccgccatt ccatagtgag ttctggcgcg     3660

cctcatccca gttggtgatg ataccgtgtt cgatggggta tttcagggtg aggatacctc     3720

ttttgctttg ggcttcatct cctacgtatg agtccttttg tcccataccg accatgactc     3780

cttgatgcct tgggcgaccg acgatcgagg ggaagacggc acggggtgcg tcatctcctg     3840

cgaaaccggc tttgcacata ccggatccat tgtctacgac aagagccgct acatcgtcgt     3900

cacacatgtt gtcttttgag gttggacact gctcactaga cagaaaaacc tcgtgccgga     3960

ccgaataccc ggtctgaacg agggcggccg cacatgagga tcacccatgt ccgcgggcta     4020

gctaagatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat     4080

ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt     4140

ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt     4200

tttgggacgc tcgaagatcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg     4260

tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg     4320

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg     4380

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa     4440

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg     4500

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc     4560

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc     4620

ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc     4680

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg     4740

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc     4800

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga     4860

gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc     4920

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac     4980

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg     5040

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc     5100

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa     5160

ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta     5220

ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt     5280

tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag     5340

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca     5400

gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc     5460

tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt     5520

tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag     5580

ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt     5640

tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat     5700

ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt     5760

gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc     5820

ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat     5880

cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag     5940

ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt     6000

ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg     6060

gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta     6120

ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc     6180

gcgcacattt ccccgaaaag tgccacctga acgaagcatc tgtgcttcat tttgtagaac     6240

aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag     6300

aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt     6360

aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt     6420

tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt     6480

tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac     6540

tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt     6600

ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg     6660

actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa     6720

aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt     6780

gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct     6840

ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa     6900

tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt     6960

agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg     7020

atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat     7080

tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc     7140

ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg     7200

aacttcggaa taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc     7260

gagctgcgca catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata     7320

tatatataca tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc     7380

gtctatttat gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg     7440

cggggtatcg tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct     7500

caattggatt agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatattaag     7560

aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc     7620


