                         SEQUENCE LISTING

<110>  National University of Singapore
 
<120>  Probe Assay For The Detection Of Biomolecules

<130>  10104SG1264

<150>  SG10201904711X
<151>  2019-05-24

<160>  77    

<170>  PatentIn version 3.5

<210>  1
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ST (split trigger)

<400>  1
gagtggatgg tgaaggtgaa ggta                                              24


<210>  2
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HP1, showing exemplary placement of the detection moiety


<220>
<221>  misc_feature
<222>  (21)..(22)
<223>  iCy5 is between residues 21 and 22

<400>  2
gttggaattg ggagtaaggg ctgtgatgcc cttactccc                              39


<210>  3
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HP2, showing exemplary placement of the detection moiety


<220>
<221>  misc_feature
<222>  (39)..(39)
<223>  Cy3 is tagged to residue 39

<400>  3
gcccttactc ccaattccaa cgggagtaag ggcatcaca                              39


<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  F (Fluorophore), showing exemplary placement of the detection 
       moiety


<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  3-FAM is tagged to residue 21

<400>  4
gttggaattg ggagtaaggg c                                                 21


<210>  5
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Q (Quencher), showing exemplary placement of the detection moiety


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5IABkFQ is tagged to residue 1

<400>  5
gcccttactc cc                                                           12


<210>  6
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I1-HP1

<400>  6
taccttcacc tttttttttt tttttttgtg cggcattcaa ttccaactgc c                51


<210>  7
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2-HP1

<400>  7
gcccttactc ctgccgcact tttttttttt ttttcaccat ccactc                      46


<210>  8
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I1-HP2

<400>  8
taccttcacc tttttttttt tttttttgtg cggcttcaat tccaacagcc                  50


<210>  9
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2-HP2

<400>  9
gcccttactc cgccgcactt tttttttttt tttcaccatc cactc                       45


<210>  10
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I1-HP3 (A4-6)

<400>  10
taccttcacc tttttttttt tttttttgtg cccttcaatt ccaacaagg                   49


<210>  11
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2-HP3 (A4-6)

<400>  11
gcccttactc cgggcacttt tttttttttt ttcaccatcc actc                        44


<210>  12
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I1-HP4

<400>  12
taccttcacc tttttttttt tttttttgtg cgccttcaat tccaacaagg c                51


<210>  13
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2-HP4

<400>  13
gcccttactc cggcgcactt tttttttttt tttcaccatc cactc                       45


<210>  14
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I1-HP5

<400>  14
taccttcacc tttttttttt tttttttgtg caggcttcaa ttccaacaag cc               52


<210>  15
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2-HP5 and I2-HP6

<400>  15
gcccttactc cgcctgcact tttttttttt ttttcaccat ccactc                      46


<210>  16
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I1-HP6

<400>  16
taccttcacc tttttttttt tttttttgtg caggcttcaa ttccaacgaa gcc              53


<210>  17
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I1-HP7 (A5-8)

<400>  17
taccttcacc tttttttttt tttttttgtg cagtgttcaa ttccaacgaa cac              53


<210>  18
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2-HP7 (A5-8) and I2-HP8

<400>  18
gcccttactc ccactgcact tttttttttt ttttcaccat ccactc                      46


<210>  19
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I1-HP8

<400>  19
taccttcacc tttttttttt tttttttgtg cagtgttcaa ttccaactga acac             54


<210>  20
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I1-HP9 (A6-9)

<400>  20
taccttcacc tttttttttt tttttttagt agtggtttca attccaacga aacc             54


<210>  21
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2-HP9 (A6-9)

<400>  21
gcccttactc caccactact tttttttttt tttttcacca tccactc                     47


<210>  22
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I1-A4

<400>  22
taccttcacc tttttttttt tttttttgtg cttcaattcc aac                         43


<210>  23
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2-A4

<400>  23
gcccttactc cgcacttttt tttttttttt caccatccac tc                          42


<210>  24
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I1-A5

<400>  24
taccttcacc tttttttttt tttttttgtg cattcaattc caac                        44


<210>  25
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2-A5

<400>  25
gcccttactc ctgcactttt tttttttttt tcaccatcca ctc                         43


<210>  26
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I1-A6

<400>  26
taccttcacc tttttttttt tttttttagt agtttcaatt ccaac                       45


<210>  27
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2-A6

<400>  27
gcccttactc cactactttt tttttttttt ttcaccatcc actc                        44


<210>  28
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PM-I1 and I1-HP9 (A6-9)

       (for single nucleotide mismatch detection)

<400>  28
gaggagatgt tttttttttt ttttagtagt ggtttcaatt ccaacgaaac c                51


<210>  29
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PM-I2 and I2-HP9 (A6-9)

       (for single nucleotide mismatch detection)

<400>  29
gcccttactc caccactact tttttttttt tttttgttgg acagccg                     47


<210>  30
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-21 (HP-I1)

<400>  30
tcaacatcag tttttttgtg cagtgttcaa ttccaacgaa cac                         43


<210>  31
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-21 (I2)

<400>  31
gcccttactc ccactgcact ttttttctga taagcta                                37


<210>  32
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-let-7a (HP-I1)

<400>  32
aactatacaa cttttttttt ttttttgtgc ttcaattcca ac                          42


<210>  33
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-let-7a (I2)

<400>  33
gcccttactc cgcacttttt tttttttttt tactacctca                             40


<210>  34
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-9 (HP-I1)

<400>  34
tcatacagct attttttttt ttttttgtgc ccttcaattc caacaagg                    48


<210>  35
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-9 (I2)

<400>  35
gcccttactc cgggcacttt tttttttttt ttgataacca aaga                        44


<210>  36
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-29 (HP-I1)

<400>  36
cggaacacca tttttttttt tttttgtgcc cttcaattcc aacaag                      46


<210>  37
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-29 (I2)

<400>  37
gcccttactc cgggcacttt tttaaagaaa tcagt                                  35


<210>  38
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 (HP-I1)

<400>  38
tgcaccccgc attacttttt tttttttttt gtgcccttca attccaacaa gg               52


<210>  39
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 (I2)

<400>  39
gcccttactc cgggcacttt tttttttttt ttgtttggtg gaccctc                     47


<210>  40
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Thrombin (HP-I1)


<400>  40
agtccgtggt agggcaggtt ggggtgactt tttttttttt ttttgtgcag tgttcaattc       60

caacgaacac                                                              70


<210>  41
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Thrombin (I2)

<400>  41
gcccttactc ccactgcact tttttttttt ttttggttgg tgtggttgg                   49


<210>  42
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I1-HP3 (A4-6)

(for protein detection)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  SH is tagged to residue 1. The thiolated end "SH-" was later 
       conjugated to antibody

<400>  42
tttttttttt tttttttttt tttttttttt gtgcccttca attccaacaa gg               52


<210>  43
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2-HP3 (A4-6)

(for protein detection)


<220>
<221>  misc_feature
<222>  (47)..(47)
<223>  SH is tagged to residue 47. The thiolated end "SH-" was later 
       conjugated to antibody

<400>  43
gcccttactc cgggcacttt tttttttttt tttttttttt ttttttt                     47


<210>  44
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I1-HP7 (A5-8)

(for protein detection)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  SH is tagged to residue 1. The thiolated end "SH-" was later 
       conjugated to antibody

<400>  44
tttttttttt tttttgtgca gtgttcaatt ccaacgaaca c                           41


<210>  45
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2-HP7 (A5-8)

(for protein detection)


<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  SH is tagged to residue 34. The thiolated end "SH-" was later 
       conjugated to antibody

<400>  45
gcccttactc ccactgcact tttttttttt tttt                                   34


<210>  46
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HP-I1 (7)

<400>  46
cctcatccct actttttttt tttttttgct agacgaattt caattccaac tgaaattcg        59


<210>  47
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2 (7)

<400>  47
gcccttactc cattcgtcta gctttttttt tttttttatc catctccac                   49


<210>  48
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HP-I1 (8) and HP-I1 (9)

<400>  48
taccttcacc tttttttttt tttttgctag acgaatttca attccaactt gaaattcg         58


<210>  49
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2 (8)

<400>  49
gcccttactc cattcgtcta gcaatttttt tttttttttc accatccact c                51


<210>  50
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2 (9)

<400>  50
gcccttactc cattcgtcta gctttttttt tttttttcac catccactc                   49


<210>  51
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HP-I1 (10)

<400>  51
taccttcacc tttttttttt tttttgctag acgaattttc aattccaact tgaaaattcg       60


<210>  52
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2 (10)

<400>  52
gcccttactc caattcgtct agcttttttt ttttttttca ccatccactc                  50


<210>  53
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HP-I1 (11)

<400>  53
taccttcacc tttttttttt tttttgctag acgaattttc aattccaact tgaaaattcg       60


<210>  54
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2 (11)

<400>  54
gcccttactc caattcgtct agcttttttt ttttttttca ccatccactc                  50


<210>  55
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HP-I1 (12)

<400>  55
taccttcacc tttttttttt tttttgctat tcgaattttc aattccaact tgaaaattcg       60


<210>  56
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2 (12)

<400>  56
gcccttactc caattcgaat agcttttttt ttttttttca ccatccactc                  50


<210>  57
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HP-I1 (13)

<400>  57
taccttcacc ttttttttac ccgattcaat tccaacaatc g                           41


<210>  58
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2 (13)

<400>  58
gcccttactc ctcgggtttt tttcaccatc cactc                                  35


<210>  59
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary target sequence for Single Nucleotide Mismatch 
       Detection (perfectly matched)

<400>  59
cggctgtcca accacatctc ctcc                                              24


<210>  60
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary target sequence for Single Nucleotide Mismatch 
       Detection (single mismatched)

<400>  60
cggctgtcca accacatctt ctcc                                              24


<210>  61
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-21

<400>  61
uagcuuauca gacugauguu ga                                                22


<210>  62
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-let-7a

<400>  62
ugagguagua gguuguauag uu                                                22


<210>  63
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-9

<400>  63
ucuuugguua ucuagcugua uga                                               23


<210>  64
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-29

<400>  64
acugauuucu uuugguguuc ag                                                22


<210>  65
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 (NC_045512.2)

<400>  65
tctggttact gccagttgaa tctgagggtc caccaaacgt aatgcggggt gcatttcgct       60

gattttgggg tc                                                           72


<210>  66
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HP-I1 (with biotin-tag)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Biotin tag is tagged to residue 1

<400>  66
tttttttttt tttttgtgca gtgttcaatt ccaacgaaca c                           41


<210>  67
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I2 (with biotin-tag)


<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  Biotin tag is tagged to residue 34

<400>  67
gcccttactc ccactgcact tttttttttt tttt                                   34


<210>  68
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Duplex-cI2

<400>  68
gtgcccggag taagggc                                                      17


<210>  69
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SM-I1

<400>  69
taccttcacc tttttttttt tttttttgtg cccttaattc caacaagg                    48


<210>  70
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Xprobe-F


<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  3Rox_N is tagged to residue 28

<400>  70
gttaaatcgt ggatagtaga cttcgcac                                          28


<210>  71
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Xprobe-Q


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5IAbRQ is tagged to residue 1

<400>  71
gtgcgaacag gtacatttgc tcgtcctt                                          28


<210>  72
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XF-P

<400>  72
aaggacgagc aaatgtacct ggcccttact cc                                     32


<210>  73
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XQ-PC

<400>  73
gttggaattg ggagtaaggg cgtctactat ccacgattta ac                          42


<210>  74
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HP1 (AuNP)

<400>  74
gttggaattg ggagtaaggg ctgtgatgcc cttactccc                              39


<210>  75
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HP2 (AuNP)

<400>  75
gcccttactc ccaattccaa cgggagtaag ggcatcaca                              39


<210>  76
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HP1 (DNAzyme)

<400>  76
gttgggaaag ttggaattgg gagtaagggc tgtgatgccc ttactccctg ggtagggcgg       60


<210>  77
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HP2 (DNAzyme)

<400>  77
gttgggaaag cccttactcc caattccaac gggagtaagg gcatcaca                    48


