<110>    MD Healthcare Inc.

<120>    Method for the diagnosis of colitis using analysis of qPCR

<130>    MPCT20-024

<150>    KR 10-2019-0061096
<151>    2019-05-24

<160>    30

<170>    KoPatentIn 3.0

<210>    1
<211>    50
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    16S_V3_F


<400>    1
tcgtcggcag cgtcagatgt gtataagaga cagcctacgg gnggcwgcag                    50


<210>    2
<211>    55
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    16S_V4_R


<400>    2
gtctcgtggg ctcggagatg tgtataagag acaggactac hvgggtatct aatcc              55


<210>    3
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Ruminococcus Forward primer


<400>    3
gtatgtaaag ctctatcagc                                                     20


<210>    4
<211>    18
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Ruminococcus Reverse primer


<400>    4
tccggtactc tagattga                                                       18


<210>    5
<211>    31
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Ruminococcus Probe: 5' is linked to a FAM and 3' is linked to a
         BHQ1


<400>    5
cccgggtttt cacatcagac ttgccactcc g                                        31


<210>    6
<211>    18
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Fusobacterium Forward primer


<400>    6
gaggaacctt accagcgt                                                       18


<210>    7
<211>    25
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Fusobacterium Reverse primer


<400>    7
ccccaactta atgatggtaa catac                                               25


<210>    8
<211>    31
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Fusobacterium Probe: 5' is linked to a FAM and 3' is linked to a
         BHQ1


<400>    8
ttaggaatga gacagagatg tttcagtgtc c                                        31


<210>    9
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Bacteroides Forward primer


<400>    9
cgggcttaaa ttgcagtgga                                                     20


<210>    10
<211>    17
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Bacteroides Reverse primer


<400>    10
tgcagcacct tcacagc                                                        17


<210>    11
<211>    30
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Bacteroides Probe: 5' is linked to a FAM and 3' is linked to a
         BHQ1


<400>    11
tgatgtggaa acatgtcagt gagcaatcac                                          30


<210>    12
<211>    17
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Acinetobacter Forward primer


<400>    12
cgaggaggag gctactt                                                        17


<210>    13
<211>    17
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Acinetobacter Reverse primer


<400>    13
cggtgcttat tctgcga                                                        17


<210>    14
<211>    30
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Acinetobacter Probe: 5' is linked to a FAM and 3' is linked to a
         BHQ1


<400>    14
tagttaatac ctagagatag tggacgttac                                          30


<210>    15
<211>    19
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Catenibacterium Forward primer


<400>    15
agtcaacgca ataagtgac                                                      19


<210>    16
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Catenibacterium Reverse primer


<400>    16
caccacctgt cttctctata                                                     20


<210>    17
<211>    30
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Catenibacterium Probe: 5' is linked to a FAM and 3' is linked to
         a BHQ1


<400>    17
gatctaaaag ggatggagac atcctcatag                                          30


<210>    18
<211>    16
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    bacterial universal 16S rDNA Forward primer


<400>    18
tgtcgtcagc tcgtgt                                                         16


<210>    19
<211>    15
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    bacterial universal 16S rDNA Reverse primer


<400>    19
gggttgcgct cgttg                                                          15


<210>    20
<211>    18
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    bacterial universal 16S rDNA Probe: 5' is linked to a FAM and 3'
         is linked to a BHQ1


<400>    20
atgttgggtt aagtcccg                                                       18


<210>    21
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Human 18S rDNA Forward primer


<400>    21
gcccgaagcg tttactttga                                                     20


<210>    22
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Human 18S rDNA Reverse primer


<400>    22
tccattattc ctagctgcgg tatc                                                24


<210>    23
<211>    19
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Human 18S rDNA Probe: 5' is linked to a FAM and 3' is linked to a
         BHQ1


<400>    23
aaagcaggcc cgagccgcc                                                      19


<210>    24
<211>    215
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Ruminococcus


<400>    24
gtatgtaaag ctctatcagc agggaagaaa atgacggtac ctgactaaga agcaccggct         60

aaatacgtgc cagcagccgc ggtaatacgt atggtgcaag cgttatccgg atttactggg        120

tgtaaaggga gcgtagacgg agtggcaagt ctgatgtgaa aacccggggc tcaaccccgg        180

gactgcattg gaaactgtca atctagagta ccgga                                   215


<210>    25
<211>    176
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Fusobacterium


<400>    25
gaggaacctt accagcgttt gacatcttag gaatgagaca gagatgtttc agtgtccctt         60

cggggaaacc taaagacagg tggtgcatgg ctgtcgtcag ctcgtgtcgt gagatgttgg        120

gttaagtccc gcaacgagcg caaccccttt cgtatgttac catcattaag ttgggg            176


<210>    26
<211>    69
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Bacteroides


<400>    26
cgggcttaaa ttgcagtgga atgatgtgga aacatgtcag tgagcaatca ccgctgtgaa         60

ggtgctgca                                                                 69


<210>    27
<211>    64
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Acinetobacter


<400>    27
cgaggaggag gctactttag ttaataccta gagatagtgg acgttactcg cagaataagc         60

accg                                                                      64


<210>    28
<211>    190
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Catenibacterium


<400>    28
agtcaacgca ataagtgacc cgcctgagta gtacgttcgc aagaatgaaa ctcaaaggaa         60

ttgacggggg cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaac        120

cttaccaggt cttgacatcg atctaaaagg gatggagaca tcctcatagc tatagagaag        180

acaggtggtg                                                               190


<210>    29
<211>    55
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    bacterial universal 16S rDNA


<400>    29
tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aaccc              55


<210>    30
<211>    81
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Human 18S rDNA


<400>    30
gcccgaagcg tttactttga aaaaattaga gtgttcaaag caggcccgag ccgcctggat         60

accgcagcta ggaataatgg a                                                   81
