                         SEQUENCE LISTING

<110>  The Governing Council of the University of Toronto
 
<120>  Methods and compositions for multiplex gene editing
  

<130>  2223-P56951PC00

<150>  GB 1907733.8
<151>  2019-05-31

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  3
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9 PAM


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or t

<400>  1
ngg                                                                      3


<210>  2
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Generic Cas9 target sequence


<220>
<221>  misc_feature
<222>  (1)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (16)..(25)
<223>  n is a, c, g, or t and can be present or absent

<220>
<221>  misc_feature
<222>  (26)..(26)
<223>  n is a, c, g, or t

<400>  2
nnnnnnnnnn nnnnnnnnnn nnnnnngg                                          28


<210>  3
<211>  4
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas12a PAM

<400>  3
tttv                                                                     4


<210>  4
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Generic Cas12a target sequence


<220>
<221>  misc_feature
<222>  (5)..(19)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (20)..(32)
<223>  n is a, c, g, or t and can be present or absent

<400>  4
tttvnnnnnn nnnnnnnnnn nnnnnnnnnn nn                                     32


<210>  5
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified S. pyogenes tracrRNA sequence (DNA)

<400>  5
gtttcagagc tatgctggaa acagcatagc aagttgaaat aaggctagtc cgttatcaac       60

ttgaaaaagt ggcaccgagt cggtgc                                            86


<210>  6
<211>  20
<212>  DNA
<213>  Lachnospiraceae bacterium

<400>  6
taatttctac tcttgtagat                                                   20


<210>  7
<211>  21
<212>  DNA
<213>  Acidaminococcus sp. BV3L6

<400>  7
taatttctac taagtgtaga t                                                 21


<210>  8
<211>  160
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Generic hgRNA for Lb-Cas12a


<220>
<221>  misc_feature
<222>  (1)..(15)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (16)..(25)
<223>  n is a, c, g, or t and can be present or absent

<220>
<221>  misc_feature
<222>  (133)..(147)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (148)..(160)
<223>  n is a, c, g, or t and can be present or absent

<400>  8
nnnnnnnnnn nnnnnnnnnn nnnnngtttc agagctatgc tggaaacagc atagcaagtt       60

gaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctaatttcta      120

ctaagtgtag atnnnnnnnn nnnnnnnnnn nnnnnnnnnn                            160


<210>  9
<211>  159
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Generic hgRNA for As-Cas12a


<220>
<221>  misc_feature
<222>  (1)..(15)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (16)..(25)
<223>  n is a, c, g, or t and can be present or absent

<220>
<221>  misc_feature
<222>  (132)..(146)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (147)..(159)
<223>  n is a, c, g, or t and can be present or absent

<400>  9
nnnnnnnnnn nnnnnnnnnn nnnnngtttc agagctatgc tggaaacagc atagcaagtt       60

gaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctaatttcta      120

ctcttgtaga tnnnnnnnnn nnnnnnnnnn nnnnnnnnn                             159


<210>  10
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Generic stuffer sequence

<400>  10
gtttagagac ggctaaatcc gcgtctcgag at                                     32


<210>  11
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Generic/degenerate stuffer sequence

<400>  11
gtttdgagac gadddddddd ccgtctcdag at                                     32


<210>  12
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Generic paired guide oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (16)..(25)
<223>  n is a, c, g, or t and can be present or absent

<220>
<221>  misc_feature
<222>  (58)..(72)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (73)..(85)
<223>  n is a, c, g, or t and can be present or absent

<400>  12
nnnnnnnnnn nnnnnnnnnn nnnnngttta gagacggcta aatccgcgtc tcgagatnnn       60

nnnnnnnnnn nnnnnnnnnn nnnnn                                             85


<210>  13
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Generic/degenerate paired guide oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (16)..(25)
<223>  n is a, c, g, or t and can be present or absent

<220>
<221>  misc_feature
<222>  (58)..(72)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (73)..(85)
<223>  n is a, c, g, or t and can be present or absent

<400>  13
nnnnnnnnnn nnnnnnnnnn nnnnngtttd gagacgaddd dddddccgtc tcdagatnnn       60

nnnnnnnnnn nnnnnnnnnn nnnnn                                             85


<210>  14
<211>  9371
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pLCHKO

<400>  14
cccgggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg       60

cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt      120

cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct      180

ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa      240

gccgcagctc acactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc      300

cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc      360

cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc      420

gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt      480

gaccgaatca ccgacctctc tccccagggg gatccaccgg agcttaccat gaccgagtac      540

aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc      600

gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag      660

cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg      720

tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg      780

ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc      840

gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc      900

ctggccaccg tcggtgtttc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg      960

ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg     1020

ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg     1080

cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac gcgttaagtc     1140

gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt     1200

gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc     1260

cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag     1320

ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc     1380

actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc     1440

cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg     1500

ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg     1560

ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc     1620

ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt     1680

cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt     1740

taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg     1800

gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt     1860

ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc     1920

ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg     1980

actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta     2040

cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat     2100

cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca     2160

tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac     2220

tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc     2280

gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat     2340

ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt     2400

ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca     2460

ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc     2520

cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc     2580

ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta     2640

agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg     2700

cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa     2760

gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc     2820

aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt     2880

cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca     2940

acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc     3000

tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta     3060

acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat     3120

ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc     3180

aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct     3240

tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag     3300

atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta     3360

agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc     3420

tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca     3480

tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg     3540

atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg     3600

ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca     3660

tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa     3720

acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa     3780

ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata     3840

aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat     3900

ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc     3960

cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata     4020

gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt     4080

actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga     4140

agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag     4200

cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa     4260

tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag     4320

agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg     4380

ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat     4440

acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta     4500

ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg     4560

gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc     4620

gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa     4680

gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc     4740

tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt     4800

caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct     4860

tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc     4920

gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg     4980

agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt     5040

ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc     5100

gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc     5160

ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct     5220

atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc     5280

tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag     5340

ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag     5400

gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac     5460

cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg     5520

gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact     5580

gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg     5640

tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag     5700

tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg     5760

actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca     5820

aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag     5880

cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa     5940

tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct     6000

ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt     6060

cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg     6120

catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa     6180

aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga     6240

tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt     6300

aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg     6360

aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc     6420

aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa     6480

tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa     6540

gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg     6600

gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg     6660

gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga     6720

aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga     6780

aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa     6840

cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg     6900

tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc     6960

attatcgttt cagacccacc tcccaacccc gaggggaccc agagaattca aaaaaaactc     7020

tgcaggtcta caaaaaagag caagaagcta aaaaagattt aaaaattatt tttagcgcag     7080

ttaatggaac aggaactaaa tttaccccaa aaatattacg tgaatcagga tataacgtta     7140

ttgaggttga agagcatgca tttgaagatg aaacatttaa aaatgttgta aatccaaatc     7200

cagaatttga tcctgcatga aaaataccgc ttgaatatgg tattaaacat gatgcagata     7260

ttattattat gaatgaccca gatgctgaca gatttggaat ggcaataaaa catgatggtc     7320

attttgtaag attagatgga aatcaaacag gaccaatttt aattgattga aaattatcaa     7380

atctaaaacg cttaaatagc attccaaaaa atccggctct atattcaagt tttgtaacaa     7440

gtgatttggg tgatagaatc gctcatgaaa aatatggagt taatattgta aaaactttaa     7500

ctggatttaa atgaatgggt agagaaattg ctaaagaaga agataacgga ttaaattttg     7560

tttttgctta tgaagaaagt tatggatatg taattgatga ctcagctaga gataaagatg     7620

gaatacaagc ttctatatta atagcagagg ctgcttgatt ttataaaaaa caaaataaaa     7680

cattagtaga ctatttagaa gatttattta aagaaatggg tgcatattac actttcactt     7740

taaacttgaa ttttaaacca gaagaaaaga aattaaaaat tgaaccatta atgaaatcat     7800

tgagagcaac acccttaact caaattgctg gacttaaagt tgttaatgtt gaagactaca     7860

tcgatggaat gtataatatg ccaggacaag acttactaaa attttattta gaagataagt     7920

catgatttgc tgttcgccca agtggaactg aacctaaact aaaaatttat tttataggtg     7980

ttggtgaatc tgttcaaaac gctaaagtta aagtagacga aattattaaa gaattaaaat     8040

taaaaatgaa tatataggag aaaaaatgaa actaaacaaa tatatagatc acacattatt     8100

aaaacaagat gctacgaaag ctgaaattaa acaattatgt gatgaagcaa ttgaatttga     8160

ttttgcaaca gtttgtgtta attcatattg aacaagctat tgtaaagaat tattaaaagg     8220

cacaaatgta ggaataacaa atgttgtagg ttttcctcta ggtgcatgca caacagctac     8280

aaaagcattc gaagtttctg aagcaattaa agatggtgca acagaaattg atatggtatt     8340

aaatattggt gcattaaaag acaaaaatta tgaattagtt ttagaagaca tgaaagctgt     8400

aaaaaaagca gctggatcac atgttgttaa atgtattatg gaaaattgtt tattaacaaa     8460

agaagaaatc atgaaagctt gtgaaatagc tgttgaagct ggattagaat ttgttaaaac     8520

atcaacagga ttttcaaaat caggtgcaac atttgaagat gttaaactaa tgaagtcagt     8580

tgttaaagac aatgctttag ttaaagcagc tggtggagtt agaacatttg aagatgctca     8640

aaaaatgatt gaagcaggag ctgaccgctt aggaacaagt ggtggagtag ctattattaa     8700

aggtgaagaa aacaacgcga gttactaaaa ctagcgtttt tttattttgc tcatttttat     8760

taaaagtttg caaaaaggaa cataaaaatt ctaattattg atactaaagt tattaaaaag     8820

aagattttgg ttgattttat aaaggtcata gaatataata ttttagcatg tgtattttgt     8880

gtgctcattt acaacacacc tgcctaccgg tacctcgtcc tttccacaag atatataaag     8940

ccaagaaatc gaaatacttt caagttacgg taagcatatg atagtccatt ttaaaacata     9000

attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta ttttgtacta     9060

atatctttgt gtttacagtc aaattaattc taattatctc tctaacagcc ttgtatcgta     9120

tatgcaaata tgaaggaatc atgggaaata ggccctctct gaattctaga tcttgagaca     9180

aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg     9240

ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat     9300

tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg     9360

gctcgagggg g                                                          9371


<210>  15
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  second oligo: 5' truncated tracrRNA and 3' truncated 
       Lb-Cas12a direct repeat

<400>  15
cagagctatg ctggaaacag catagcaagt tgaaataagg ctagtccgtt atcaacttga       60

aaaagtggca ccgagtcggt gctaatttct actaagtgt                              99


<210>  16
<211>  98
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  second oligo: 5  truncated tracrRNA and 3' truncated 
       As-Cas12a direct repeat 

<400>  16
cagagctatg ctggaaacag catagcaagt tgaaataagg ctagtccgtt atcaacttga       60

aaaagtggca ccgagtcggt gctaatttct actcttgt                               98


<210>  17
<211>  122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BsmBI - tracrRNA - Lb-Cas12a_DR - BsmBI

<400>  17
cgtctctgtt tcagagctat gctggaaaca gcatagcaag ttgaaataag gctagtccgt       60

tatcaacttg aaaaagtggc accgagtcgg tgcttaattt ctactaagtg tagatagaga      120

cg                                                                     122


<210>  18
<211>  121
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BsmBI - tracrRNA - As-Cas12a_DR - BsmBI

<400>  18
cgtctctgtt tcagagctat gctggaaaca gcatagcaag ttgaaataag gctagtccgt       60

tatcaacttg aaaaagtggc accgagtcgg tgcttaattt ctactcttgt agatagagac      120

g                                                                      121


<210>  19
<211>  4128
<212>  DNA
<213>  Streptococcus pyogenes

<400>  19
atgggtatcc acggagtccc agcagccgac aagaagtaca gcatcggcct ggacatcggc       60

accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc      120

aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg      180

ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac      240

accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag      300

gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag      360

cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac      420

cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg      480

ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc      540

gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac      600

aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg      660

tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct gcccggcgag      720

aagaagaatg gcctgttcgg caacctgatt gccctgagcc tgggcctgac ccccaacttc      780

aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga cacctacgac      840

gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct gtttctggcc      900

gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa caccgagatc      960

accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca ccaggacctg     1020

accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga gattttcttc     1080

gaccagagca agaacggcta cgccggctac attgacggcg gagccagcca ggaagagttc     1140

tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact gctcgtgaag     1200

ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag catcccccac     1260

cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt ttacccattc     1320

ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc ctactacgtg     1380

ggccctctgg ccaggggaaa cagcagattc gcctggatga ccagaaagag cgaggaaacc     1440

atcaccccct ggaacttcga ggaagtggtg gacaagggcg cttccgccca gagcttcatc     1500

gagcggatga ccaacttcga taagaacctg cccaacgaga aggtgctgcc caagcacagc     1560

ctgctgtacg agtacttcac cgtgtataac gagctgacca aagtgaaata cgtgaccgag     1620

ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa aggccatcgt ggacctgctg     1680

ttcaagacca accggaaagt gaccgtgaag cagctgaaag aggactactt caagaaaatc     1740

gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc ctccctgggc     1800

acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa tgaggaaaac     1860

gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag agagatgatc     1920

gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag     1980

cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg catccgggac     2040

aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc caacagaaac     2100

ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca gaaagcccag     2160

gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg cagccccgcc     2220

attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa agtgatgggc     2280

cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac cacccagaag     2340

ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa agagctgggc     2400

agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga gaagctgtac     2460

ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga catcaaccgg     2520

ctgtccgact acgatgtgga ccatatcgtg cctcagagct ttctgaagga cgactccatc     2580

gacaacaagg tgctgaccag aagcgacaag aaccggggca agagcgacaa cgtgccctcc     2640

gaagaggtcg tgaagaagat gaagaactac tggcggcagc tgctgaacgc caagctgatt     2700

acccagagaa agttcgacaa tctgaccaag gccgagagag gcggcctgag cgaactggat     2760

aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa gcacgtggca     2820

cagatcctgg actcccggat gaacactaag tacgacgaga atgacaagct gatccgggaa     2880

gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga tttccagttt     2940

tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct gaacgccgtc     3000

gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt gtacggcgac     3060

tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat cggcaaggct     3120

accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga gattaccctg     3180

gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac cggggagatc     3240

gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat gccccaagtg     3300

aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc     3360

aagaggaaca gcgataagct gatcgccaga aagaaggact gggaccctaa gaagtacggc     3420

ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt ggaaaagggc     3480

aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc     3540

agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga agtgaaaaag     3600

gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg ccggaagaga     3660

atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc ctccaaatat     3720

gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc cgaggataat     3780

gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat catcgagcag     3840

atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc     3900

gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg     3960

tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac caccatcgac     4020

cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc     4080

accggcctgt acgagacacg gatcgacctg tctcagctgg gaggcgac                  4128


<210>  20
<211>  3684
<212>  DNA
<213>  Lachnospiraceae bacterium

<400>  20
atgagcaagc tggagaagtt tacaaactgc tactccctgt ctaagaccct gaggttcaag       60

gccatccctg tgggcaagac ccaggagaac atcgacaata agcggctgct ggtggaggac      120

gagaagagag ccgaggatta taagggcgtg aagaagctgc tggatcgcta ctatctgtct      180

tttatcaacg acgtgctgca cagcatcaag ctgaagaatc tgaacaatta catcagcctg      240

ttccggaaga aaaccagaac cgagaaggag aataaggagc tggagaacct ggagatcaat      300

ctgcggaagg agatcgccaa ggccttcaag ggcaacgagg gctacaagtc cctgtttaag      360

aaggatatca tcgagacaat cctgccagag ttcctggacg ataaggacga gatcgccctg      420

gtgaacagct tcaatggctt taccacagcc ttcaccggct tctttgataa cagagagaat      480

atgttttccg aggaggccaa gagcacatcc atcgccttca ggtgtatcaa cgagaatctg      540

acccgctaca tctctaatat ggacatcttc gagaaggtgg acgccatctt tgataagcac      600

gaggtgcagg agatcaagga gaagatcctg aacagcgact atgatgtgga ggatttcttt      660

gagggcgagt tctttaactt tgtgctgaca caggagggca tcgacgtgta taacgccatc      720

atcggcggct tcgtgaccga gagcggcgag aagatcaagg gcctgaacga gtacatcaac      780

ctgtataatc agaaaaccaa gcagaagctg cctaagttta agccactgta taagcaggtg      840

ctgagcgatc gggagtctct gagcttctac ggcgagggct atacatccga tgaggaggtg      900

ctggaggtgt ttagaaacac cctgaacaag aacagcgaga tcttcagctc catcaagaag      960

ctggagaagc tgttcaagaa ttttgacgag tactctagcg ccggcatctt tgtgaagaac     1020

ggccccgcca tcagcacaat ctccaaggat atcttcggcg agtggaacgt gatccgggac     1080

aagtggaatg ccgagtatga cgatatccac ctgaagaaga aggccgtggt gaccgagaag     1140

tacgaggacg atcggagaaa gtccttcaag aagatcggct ccttttctct ggagcagctg     1200

caggagtacg ccgacgccga tctgtctgtg gtggagaagc tgaaggagat catcatccag     1260

aaggtggatg agatctacaa ggtgtatggc tcctctgaga agctgttcga cgccgatttt     1320

gtgctggaga agagcctgaa gaagaacgac gccgtggtgg ccatcatgaa ggacctgctg     1380

gattctgtga agagcttcga gaattacatc aaggccttct ttggcgaggg caaggagaca     1440

aacagggacg agtccttcta tggcgatttt gtgctggcct acgacatcct gctgaaggtg     1500

gaccacatct acgatgccat ccgcaattat gtgacccaga agccctactc taaggataag     1560

ttcaagctgt attttcagaa ccctcagttc atgggcggct gggacaagga taaggagaca     1620

gactatcggg ccaccatcct gagatacggc tccaagtact atctggccat catggataag     1680

aagtacgcca agtgcctgca gaagatcgac aaggacgatg tgaacggcaa ttacgagaag     1740

atcaactata agctgctgcc cggccctaat aagatgctgc caaaggtgtt cttttctaag     1800

aagtggatgg cctactataa ccccagcgag gacatccaga agatctacaa gaatggcaca     1860

ttcaagaagg gcgatatgtt taacctgaat gactgtcaca agctgatcga cttctttaag     1920

gatagcatct cccggtatcc aaagtggtcc aatgcctacg atttcaactt ttctgagaca     1980

gagaagtata aggacatcgc cggcttttac agagaggtgg aggagcaggg ctataaggtg     2040

agcttcgagt ctgccagcaa gaaggaggtg gataagctgg tggaggaggg caagctgtat     2100

atgttccaga tctataacaa ggacttttcc gataagtctc acggcacacc caatctgcac     2160

accatgtact tcaagctgct gtttgacgag aacaatcacg gacagatcag gctgagcgga     2220

ggagcagagc tgttcatgag gcgcgcctcc ctgaagaagg aggagctggt ggtgcaccca     2280

gccaactccc ctatcgccaa caagaatcca gataatccca agaaaaccac aaccctgtcc     2340

tacgacgtgt ataaggataa gaggttttct gaggaccagt acgagctgca catcccaatc     2400

gccatcaata agtgccccaa gaacatcttc aagatcaata cagaggtgcg cgtgctgctg     2460

aagcacgacg ataaccccta tgtgatcggc atcgataggg gcgagcgcaa tctgctgtat     2520

atcgtggtgg tggacggcaa gggcaacatc gtggagcagt attccctgaa cgagatcatc     2580

aacaacttca acggcatcag gatcaagaca gattaccact ctctgctgga caagaaggag     2640

aaggagaggt tcgaggcccg ccagaactgg acctccatcg agaatatcaa ggagctgaag     2700

gccggctata tctctcaggt ggtgcacaag atctgcgagc tggtggagaa gtacgatgcc     2760

gtgatcgccc tggaggacct gaactctggc tttaagaata gccgcgtgaa ggtggagaag     2820

caggtgtatc agaagttcga gaagatgctg atcgataagc tgaactacat ggtggacaag     2880

aagtctaatc cttgtgcaac aggcggcgcc ctgaagggct atcagatcac caataagttc     2940

gagagcttta agtccatgtc tacccagaac ggcttcatct tttacatccc tgcctggctg     3000

acatccaaga tcgatccatc taccggcttt gtgaacctgc tgaaaaccaa gtataccagc     3060

atcgccgatt ccaagaagtt catcagctcc tttgacagga tcatgtacgt gcccgaggag     3120

gatctgttcg agtttgccct ggactataag aacttctctc gcacagacgc cgattacatc     3180

aagaagtgga agctgtactc ctacggcaac cggatcagaa tcttccggaa tcctaagaag     3240

aacaacgtgt tcgactggga ggaggtgtgc ctgaccagcg cctataagga gctgttcaac     3300

aagtacggca tcaattatca gcagggcgat atcagagccc tgctgtgcga gcagtccgac     3360

aaggccttct actctagctt tatggccctg atgagcctga tgctgcagat gcggaacagc     3420

atcacaggcc gcaccgacgt ggattttctg atcagccctg tgaagaactc cgacggcatc     3480

ttctacgata gccggaacta tgaggcccag gagaatgcca tcctgccaaa gaacgccgac     3540

gccaatggcg cctataacat cgccagaaag gtgctgtggg ccatcggcca gttcaagaag     3600

gccgaggacg agaagctgga taaggtgaag atcgccatct ctaacaagga gtggctggag     3660

tacgcccaga ccagcgtgaa gcac                                            3684


<210>  21
<211>  3921
<212>  DNA
<213>  Acidaminococcus sp. BV3L6

<400>  21
atgacacagt tcgagggctt taccaacctg tatcaggtga gcaagacact gcggtttgag       60

ctgatcccac agggcaagac cctgaagcac atccaggagc agggcttcat cgaggaggac      120

aaggcccgca atgatcacta caaggagctg aagcccatca tcgatcggat ctacaagacc      180

tatgccgacc agtgcctgca gctggtgcag ctggattggg agaacctgag cgccgccatc      240

gactcctata gaaaggagaa aaccgaggag acaaggaacg ccctgatcga ggagcaggcc      300

acatatcgca atgccatcca cgactacttc atcggccgga cagacaacct gaccgatgcc      360

atcaataaga gacacgccga gatctacaag ggcctgttca aggccgagct gtttaatggc      420

aaggtgctga agcagctggg caccgtgacc acaaccgagc acgagaacgc cctgctgcgg      480

agcttcgaca agtttacaac ctacttctcc ggcttttatg agaacaggaa gaacgtgttc      540

agcgccgagg atatcagcac agccatccca caccgcatcg tgcaggacaa cttccccaag      600

tttaaggaga attgtcacat cttcacacgc ctgatcaccg ccgtgcccag cctgcgggag      660

cactttgaga acgtgaagaa ggccatcggc atcttcgtga gcacctccat cgaggaggtg      720

ttttccttcc ctttttataa ccagctgctg acacagaccc agatcgacct gtataaccag      780

ctgctgggag gaatctctcg ggaggcaggc accgagaaga tcaagggcct gaacgaggtg      840

ctgaatctgg ccatccagaa gaatgatgag acagcccaca tcatcgcctc cctgccacac      900

agattcatcc ccctgtttaa gcagatcctg tccgatagga acaccctgtc tttcatcctg      960

gaggagttta agagcgacga ggaagtgatc cagtccttct gcaagtacaa gacactgctg     1020

agaaacgaga acgtgctgga gacagccgag gccctgttta acgagctgaa cagcatcgac     1080

ctgacacaca tcttcatcag ccacaagaag ctggagacaa tcagcagcgc cctgtgcgac     1140

cactgggata cactgaggaa tgccctgtat gagcggagaa tctccgagct gacaggcaag     1200

atcaccaagt ctgccaagga gaaggtgcag cgcagcctga agcacgagga tatcaacctg     1260

caggagatca tctctgccgc aggcaaggag ctgagcgagg ccttcaagca gaaaaccagc     1320

gagatcctgt cccacgcaca cgccgccctg gatcagccac tgcctacaac cctgaagaag     1380

caggaggaga aggagatcct gaagtctcag ctggacagcc tgctgggcct gtaccacctg     1440

ctggactggt ttgccgtgga tgagtccaac gaggtggacc ccgagttctc tgcccggctg     1500

accggcatca agctggagat ggagccttct ctgagcttct acaacaaggc cagaaattat     1560

gccaccaaga agccctactc cgtggagaag ttcaagctga actttcagat gcctacactg     1620

gcctctggct gggacgtgaa taaggagaag aacaatggcg ccatcctgtt tgtgaagaac     1680

ggcctgtact atctgggcat catgccaaag cagaagggca ggtataaggc cctgagcttc     1740

gagcccacag agaaaaccag cgagggcttt gataagatgt actatgacta cttccctgat     1800

gccgccaaga tgatcccaaa gtgcagcacc cagctgaagg ccgtgacagc ccactttcag     1860

acccacacaa cccccatcct gctgtccaac aatttcatcg agcctctgga gatcacaaag     1920

gagatctacg acctgaacaa tcctgagaag gagccaaaga agtttcagac agcctacgcc     1980

aagaaaaccg gcgaccagaa gggctacaga gaggccctgt gcaagtggat cgacttcaca     2040

agggattttc tgtccaagta taccaagaca acctctatcg atctgtctag cctgcggcca     2100

tcctctcagt ataaggacct gggcgagtac tatgccgagc tgaatcccct gctgtaccac     2160

atcagcttcc agagaatcgc cgagaaggag atcatggatg ccgtggagac aggcaagctg     2220

tacctgttcc agatctataa caaggacttt gccaagggcc accacggcaa gcctaatctg     2280

cacacactgt attggaccgg cctgttttct ccagagaacc tggccaagac aagcatcaag     2340

ctgaatggcc aggccgagct gttctaccgc cctaagtcca ggatgaagag gatggcacac     2400

cggctgggag agaagatgct gaacaagaag ctgaaggatc agaaaacccc aatccccgac     2460

accctgtacc aggagctgta cgactatgtg aatcacagac tgtcccacga cctgtctgat     2520

gaggccaggg ccctgctgcc caacgtgatc accaaggagg tgtctcacga gatcatcaag     2580

gataggcgct ttaccagcga caagttcttt ttccacgtgc ctatcacact gaactatcag     2640

gccgccaatt ccccatctaa gttcaaccag agggtgaatg cctacctgaa ggagcacccc     2700

gagacaccta tcatcggcat cgatcggggc gagagaaacc tgatctatat cacagtgatc     2760

gactccaccg gcaagatcct ggagcagcgg agcctgaaca ccatccagca gtttgattac     2820

cagaagaagc tggacaacag ggagaaggag agggtggcag caaggcaggc ctggtctgtg     2880

gtgggcacaa tcaaggatct gaagcagggc tatctgagcc aggtcatcca cgagatcgtg     2940

gacctgatga tccactacca ggccgtggtg gtgctggaga acctgaattt cggctttaag     3000

agcaagagga ccggcatcgc cgagaaggcc gtgtaccagc agttcgagaa gatgctgatc     3060

gataagctga attgcctggt gctgaaggac tatccagcag agaaagtggg aggcgtgctg     3120

aacccatacc agctgacaga ccagttcacc tcctttgcca agatgggcac ccagtctggc     3180

ttcctgtttt acgtgcctgc cccatataca tctaagatcg atcccctgac cggcttcgtg     3240

gaccccttcg tgtggaaaac catcaagaat cacgagagcc gcaagcactt cctggagggc     3300

ttcgactttc tgcactacga cgtgaaaacc ggcgacttca tcctgcactt taagatgaac     3360

agaaatctgt ccttccagag gggcctgccc ggctttatgc ctgcatggga tatcgtgttc     3420

gagaagaacg agacacagtt tgacgccaag ggcacccctt tcatcgccgg caagagaatc     3480

gtgccagtga tcgagaatca cagattcacc ggcagatacc gggacctgta tcctgccaac     3540

gagctgatcg ccctgctgga ggagaagggc atcgtgttca gggatggctc caacatcctg     3600

ccaaagctgc tggagaatga cgattctcac gccatcgaca ccatggtggc cctgatccgc     3660

agcgtgctgc agatgcggaa ctccaatgcc gccacaggcg aggactatat caacagcccc     3720

gtgcgcgatc tgaatggcgt gtgcttcgac tcccggtttc agaacccaga gtggcccatg     3780

gacgccgatg ccaatggcgc ctaccacatc gccctgaagg gccagctgct gctgaatcac     3840

ctgaaggaga gcaaggatct gaagctgcag aacggcatct ccaatcagga ctggctggcc     3900

tacatccagg agctgcgcaa c                                               3921


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SV40 NLS 

<400>  22
ccaaagaaga agcggaaggt c                                                 21


<210>  23
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleoplasmin NLS 

<400>  23
aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaag                    48


<210>  24
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Myc tag 

<400>  24
gaacaaaaac tcatctcaga agaggatctg                                        30


<210>  25
<211>  113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CHIP Oligo


<220>
<221>  misc_feature
<222>  (20)..(39)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (72)..(94)
<223>  n is a, c, g, or t

<400>  25
agagaacctg cagagaccgn nnnnnnnnnn nnnnnnnnng tttagagacg gctaaatccg       60

cgtctcgaga tnnnnnnnnn nnnnnnnnnn nnnnttttag aggcaggtag aga             113


<210>  26
<211>  113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CHIP Oligo with degenerate nucleotide code


<220>
<221>  misc_feature
<222>  (20)..(39)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (72)..(94)
<223>  n is a, c, g, or t

<400>  26
agagaacctg cagagaccgn nnnnnnnnnn nnnnnnnnng tttdgagacg addddddddc       60

cgtctcdaga tnnnnnnnnn nnnnnnnnnn nnnnttttag aggcaggtag aga             113


<210>  27
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TOPO fragment As-Cas12a

<400>  27
cgtctctgtt tcagagctat gctggaaaca gcatagcaag ttgaaataag gctagtccgt       60

tatcaacttg aaaaagtggc accgagtcgg tgctaatttc tactcttgta gatagagacg      120


<210>  28
<211>  121
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TOPO fragment Lb-Cas12a

<400>  28
cgtctctgtt tcagagctat gctggaaaca gcatagcaag ttgaaataag gctagtccgt       60

tatcaacttg aaaaagtggc accgagtcgg tgctaatttc tactaagtgt agatagagac      120

g                                                                      121


<210>  29
<211>  171
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  As-Cas12a hgRNA insert


<220>
<221>  misc_feature
<222>  (14)..(33)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (140)..(162)
<223>  n is a, c, g, t or u

<400>  29
ggacgaggta ccgnnnnnnn nnnnnnnnnn nnngtttcag agctatgctg gaaacagcat       60

agcaagttga aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct      120

aatttctact cttgtagatn nnnnnnnnnn nnnnnnnnnn nntttttttt t               171


<210>  30
<211>  172
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lb-Cas12a hgRNA insert


<220>
<221>  misc_feature
<222>  (14)..(33)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (141)..(163)
<223>  n is a, c, g, t or u

<400>  30
ggacgaggta ccgnnnnnnn nnnnnnnnnn nnngtttcag agctatgctg gaaacagcat       60

agcaagttga aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct      120

aatttctact aagtgtagat nnnnnnnnnn nnnnnnnnnn nnnttttttt tt              172


