                         SEQUENCE LISTING

<110>  Etablissement Franais du Sang
       INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE 
       (INSERM)
       CENTRE HOSPITALIER UNIVERSITAIRE DE BESANCON
       UNIVERSITE DE FRANCHE COMTE
 
<120>  CAR-T CELLS TARGETING IL-1RAP AND THEIR USE IN AML

<130>  1H318460 - 0005

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  423
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence coding chain H (VH) of murine scFv 
       anti-IL-1RAP

<400>  1
atgggatgga gctgtatcat cctcttcttg gtagcaacag ctacaggtgt caactcccag       60

gtccaactgc agcagcctgg ggctgagctt atgatgcctg gggcttcagt gaaagtgtcc      120

tgcgaggctt ctggctacac attcactgac tcctggatgc actgggtgaa gcagaggcct      180

ggacaaggcc ttgagtggat cggagcgatt gatccttctg atagttatac tacctataat      240

caaaaattca cgggcaaggc cacattgagt gtagacgaat cctccaacac agcctacatg      300

cagctcagca gcctgacatc tgaggactct gcggtctatt actgtgcaag gtattactcc      360

ggtagtaact acatatcgcc ctttccttac tggggccaag ggactctggt cactgtctct      420

gca                                                                    423


<210>  2
<211>  141
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of chain H (VH) of murine scFv anti-IL-1RAP

<400>  2

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val Asn Ser Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Met Met 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Val Ser Cys Glu Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Ser Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Ala Ile Asp Pro Ser Asp Ser Tyr Thr Thr Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Thr Gly Lys Ala Thr Leu Ser Val Asp Glu Ser Ser Asn 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Tyr Tyr Ser Gly Ser Asn Tyr Ile Ser Pro Phe 
        115                 120                 125             


Pro Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala 
    130                 135                 140     


<210>  3
<211>  382
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence coding chain K (VL) of murine scFv 
       anti-IL-1RAP

<400>  3
atggagtcac agattcaggt ctttgtattc gtgtttctct ggttgtctgg tgttgacgga       60

gacattgtga tgacccagtc tcacaaattc atgtccacat cagtaggaga cagggtcacc      120

atcacctgca aggccagtct ggatgtgagt actgctgtgg cctggtatca acagaaacca      180

ggacaatctc ctaaactact gatttactcg gcatcctacc ggtacactgg agtccctgat      240

cgcttcactg gcagtggatc tgggacggat ttcactttca ccatcagcag tgtgcaggct      300

gaagacctgg cagtttatta ctgtcagcaa cattatagtc ctccattcac gttcggctcg      360

gggacaaact tggagataaa ac                                               382


<210>  4
<211>  127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of chain K (VL) of murine scFv anti-IL-1RAP

<400>  4

Met Glu Ser Gln Ile Gln Val Phe Val Phe Val Phe Leu Trp Leu Ser 
1               5                   10                  15      


Gly Val Asp Gly Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser 
            20                  25                  30          


Thr Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Leu Asp 
        35                  40                  45              


Val Ser Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro 
    50                  55                  60                  


Lys Leu Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Asp 
65                  70                  75                  80  


Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser 
                85                  90                  95      


Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln His Tyr 
            100                 105                 110         


Ser Pro Pro Phe Thr Phe Gly Ser Gly Thr Asn Leu Glu Ile Lys 
        115                 120                 125         


<210>  5
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker between the VH and VL domains

<400>  5

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Val Asp 
1               5                   10                  15  


<210>  6
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1 of the light chain

<400>  6

Leu Asp Val Ser Thr Ala 
1               5       


<210>  7
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2 of the light chain

<400>  7

Ser Ala Ser 
1           


<210>  8
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3 of the light chain

<400>  8

Gln Gln His Tyr Ser Pro Pro Phe Thr 
1               5                   


<210>  9
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDR1 of the light chain (nt)

<400>  9
ctggatgtga gtactgct                                                     18


<210>  10
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDR2 of the light chain (nt)

<400>  10
tcggcatcc                                                                9


<210>  11
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDR3 of the light chain (nt)

<400>  11
cagcaacatt atagtcctcc attcacg                                           27


<210>  12
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1 of the heavy chain

<400>  12

Gly Tyr Thr Phe Thr Asp Ser Trp 
1               5               


<210>  13
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2 of the heavy chain

<400>  13

Ile Asp Pro Ser Asp Ser Tyr Thr 
1               5               


<210>  14
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3 of the heavy chain

<400>  14

Ala Arg Tyr Tyr Ser Gly Ser Asn Tyr Ile Ser Pro Phe Pro Tyr 
1               5                   10                  15  


<210>  15
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDR1 of the heavy chain (nt)

<400>  15
ggctacacat tcactgactc ctgg                                              24


<210>  16
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDR2 of the heavy chain (nt)

<400>  16
attgatcctt ctgatagtta tact                                              24


<210>  17
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDR3 of the heavy chain (nt)

<400>  17
gcaaggtatt actccggtag taactacata tcgccctttc cttac                       45


<210>  18
<211>  283
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of murine scFv anti-IL-1RAP (i.e. #A3C3 CAR)

<400>  18

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val Asn Ser Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Met Met 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Val Ser Cys Glu Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Ser Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Ala Ile Asp Pro Ser Asp Ser Tyr Thr Thr Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Thr Gly Lys Ala Thr Leu Ser Val Asp Glu Ser Ser Asn 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Tyr Tyr Ser Gly Ser Asn Tyr Ile Ser Pro Phe 
        115                 120                 125             


Pro Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Val Asp Met Glu Ser Gln 
145                 150                 155                 160 


Ile Gln Val Phe Val Phe Val Phe Leu Trp Leu Ser Gly Val Asp Gly 
                165                 170                 175     


Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser Val Gly 
            180                 185                 190         


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Leu Asp Val Ser Thr Ala 
        195                 200                 205             


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
    210                 215                 220                 


Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly 
225                 230                 235                 240 


Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Val Gln Ala 
                245                 250                 255     


Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln His Tyr Ser Pro Pro Phe 
            260                 265                 270         


Thr Phe Gly Ser Gly Thr Asn Leu Glu Ile Lys 
        275                 280             


