                         SEQUENCE LISTING

<110>  Christiana Care Health Services, Inc.
       Kmiec, Eric
       Bialk, Pawel
 
<120>  Gene Knockout of NRF2 for Treatment of Cancer

<130>  130949-00101

<150>  62/852,076
<151>  2019-05-23

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA recognition element

<400>  1
agctactctg gcccttatag tcc                                               23


<210>  2
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  2
ucgaugugac cgggaauauc agg                                               23


<210>  3
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA recognition element

<400>  3
actaaatctg ccatacgttg tcc                                               23


<210>  4
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  4
ugauuuagac gguaugcaac agg                                               23


<210>  5
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
gtagtggtgc cttagagctt actcatcc                                          28


<210>  6
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
ctagcatggg cagtactcat gactaag                                           27


<210>  7
<211>  2446
<212>  DNA
<213>  Homo sapiens

<400>  7
gattaccgag tgccggggag cccggaggag ccgccgacgc agccgccacc gccgccgccg       60

ccgccaccag agccgccctg tccgcgccgc gcctcggcag ccggaacagg gccgccgtcg      120

gggagcccca acacacggtc cacagctcat catgatggac ttggagctgc cgccgccggg      180

actcccgtcc cagcaggaca tggatttgat tgacatactt tggaggcaag atatagatct      240

tggagtaagt cgagaagtat ttgacttcag tcagcgacgg aaagagtatg agctggaaaa      300

acagaaaaaa cttgaaaagg aaagacaaga acaactccaa aaggagcaag agaaagcctt      360

tttcgctcag ttacaactag atgaagagac aggtgaattt ctcccaattc agccagccca      420

gcacatccag tcagaaacca gtggatctgc caactactcc caggttgccc acattcccaa      480

atcagatgct ttgtactttg atgactgcat gcagcttttg gcgcagacat tcccgtttgt      540

agatgacaat gaggtttctt cggctacgtt tcagtcactt gttcctgata ttcccggtca      600

catcgagagc ccagtcttca ttgctactaa tcaggctcag tcacctgaaa cttctgttgc      660

tcaggtagcc cctgttgatt tagacggtat gcaacaggac attgagcaag tttgggagga      720

gctattatcc attcctgagt tacagtgtct taatattgaa aatgacaagc tggttgagac      780

taccatggtt ccaagtccag aagccaaact gacagaagtt gacaattatc atttttactc      840

atctataccc tcaatggaaa aagaagtagg taactgtagt ccacattttc ttaatgcttt      900

tgaggattcc ttcagcagca tcctctccac agaagacccc aaccagttga cagtgaactc      960

attaaattca gatgccacag tcaacacaga ttttggtgat gaattttatt ctgctttcat     1020

agctgagccc agtatcagca acagcatgcc ctcacctgct actttaagcc attcactctc     1080

tgaacttcta aatgggccca ttgatgtttc tgatctatca ctttgcaaag ctttcaacca     1140

aaaccaccct gaaagcacag cagaattcaa tgattctgac tccggcattt cactaaacac     1200

aagtcccagt gtggcatcac cagaacactc agtggaatct tccagctatg gagacacact     1260

acttggcctc agtgattctg aagtggaaga gctagatagt gcccctggaa gtgtcaaaca     1320

gaatggtcct aaaacaccag tacattcttc tggggatatg gtacaaccct tgtcaccatc     1380

tcaggggcag agcactcacg tgcatgatgc ccaatgtgag aacacaccag agaaagaatt     1440

gcctgtaagt cctggtcatc ggaaaacccc attcacaaaa gacaaacatt caagccgctt     1500

ggaggctcat ctcacaagag atgaacttag ggcaaaagct ctccatatcc cattccctgt     1560

agaaaaaatc attaacctcc ctgttgttga cttcaacgaa atgatgtcca aagagcagtt     1620

caatgaagct caacttgcat taattcggga tatacgtagg aggggtaaga ataaagtggc     1680

tgctcagaat tgcagaaaaa gaaaactgga aaatatagta gaactagagc aagatttaga     1740

tcatttgaaa gatgaaaaag aaaaattgct caaagaaaaa ggagaaaatg acaaaagcct     1800

tcacctactg aaaaaacaac tcagcacctt atatctcgaa gttttcagca tgctacgtga     1860

tgaagatgga aaaccttatt ctcctagtga atactccctg cagcaaacaa gagatggcaa     1920

tgttttcctt gttcccaaaa gtaagaagcc agatgttaag aaaaactaga tttaggagga     1980

tttgaccttt tctgagctag tttttttgta ctattatact aaaagctcct actgtgatgt     2040

gaaatgctca tactttataa gtaattctat gcaaaatcat agccaaaact agtatagaaa     2100

ataatacgaa actttaaaaa gcattggagt gtcagtatgt tgaatcagta gtttcacttt     2160

aactgtaaac aatttcttag gacaccattt gggctagttt ctgtgtaagt gtaaatacta     2220

caaaaactta tttatactgt tcttatgtca tttgttatat tcatagattt atatgatgat     2280

atgacatctg gctaaaaaga aattattgca aaactaacca ctatgtactt ttttataaat     2340

actgtatgga caaaaaatgg cattttttat attaaattgt ttagctctgg caaaaaaaaa     2400

aaattttaag agctggtact aataaaggat tattatgact gttaaa                    2446


<210>  8
<211>  605
<212>  PRT
<213>  Homo sapiens

<400>  8

Met Met Asp Leu Glu Leu Pro Pro Pro Gly Leu Pro Ser Gln Gln Asp 
1               5                   10                  15      


Met Asp Leu Ile Asp Ile Leu Trp Arg Gln Asp Ile Asp Leu Gly Val 
            20                  25                  30          


Ser Arg Glu Val Phe Asp Phe Ser Gln Arg Arg Lys Glu Tyr Glu Leu 
        35                  40                  45              


Glu Lys Gln Lys Lys Leu Glu Lys Glu Arg Gln Glu Gln Leu Gln Lys 
    50                  55                  60                  


Glu Gln Glu Lys Ala Phe Phe Ala Gln Leu Gln Leu Asp Glu Glu Thr 
65                  70                  75                  80  


Gly Glu Phe Leu Pro Ile Gln Pro Ala Gln His Ile Gln Ser Glu Thr 
                85                  90                  95      


Ser Gly Ser Ala Asn Tyr Ser Gln Val Ala His Ile Pro Lys Ser Asp 
            100                 105                 110         


Ala Leu Tyr Phe Asp Asp Cys Met Gln Leu Leu Ala Gln Thr Phe Pro 
        115                 120                 125             


Phe Val Asp Asp Asn Glu Val Ser Ser Ala Thr Phe Gln Ser Leu Val 
    130                 135                 140                 


Pro Asp Ile Pro Gly His Ile Glu Ser Pro Val Phe Ile Ala Thr Asn 
145                 150                 155                 160 


Gln Ala Gln Ser Pro Glu Thr Ser Val Ala Gln Val Ala Pro Val Asp 
                165                 170                 175     


Leu Asp Gly Met Gln Gln Asp Ile Glu Gln Val Trp Glu Glu Leu Leu 
            180                 185                 190         


Ser Ile Pro Glu Leu Gln Cys Leu Asn Ile Glu Asn Asp Lys Leu Val 
        195                 200                 205             


Glu Thr Thr Met Val Pro Ser Pro Glu Ala Lys Leu Thr Glu Val Asp 
    210                 215                 220                 


Asn Tyr His Phe Tyr Ser Ser Ile Pro Ser Met Glu Lys Glu Val Gly 
225                 230                 235                 240 


Asn Cys Ser Pro His Phe Leu Asn Ala Phe Glu Asp Ser Phe Ser Ser 
                245                 250                 255     


Ile Leu Ser Thr Glu Asp Pro Asn Gln Leu Thr Val Asn Ser Leu Asn 
            260                 265                 270         


Ser Asp Ala Thr Val Asn Thr Asp Phe Gly Asp Glu Phe Tyr Ser Ala 
        275                 280                 285             


Phe Ile Ala Glu Pro Ser Ile Ser Asn Ser Met Pro Ser Pro Ala Thr 
    290                 295                 300                 


Leu Ser His Ser Leu Ser Glu Leu Leu Asn Gly Pro Ile Asp Val Ser 
305                 310                 315                 320 


Asp Leu Ser Leu Cys Lys Ala Phe Asn Gln Asn His Pro Glu Ser Thr 
                325                 330                 335     


Ala Glu Phe Asn Asp Ser Asp Ser Gly Ile Ser Leu Asn Thr Ser Pro 
            340                 345                 350         


Ser Val Ala Ser Pro Glu His Ser Val Glu Ser Ser Ser Tyr Gly Asp 
        355                 360                 365             


Thr Leu Leu Gly Leu Ser Asp Ser Glu Val Glu Glu Leu Asp Ser Ala 
    370                 375                 380                 


Pro Gly Ser Val Lys Gln Asn Gly Pro Lys Thr Pro Val His Ser Ser 
385                 390                 395                 400 


Gly Asp Met Val Gln Pro Leu Ser Pro Ser Gln Gly Gln Ser Thr His 
                405                 410                 415     


Val His Asp Ala Gln Cys Glu Asn Thr Pro Glu Lys Glu Leu Pro Val 
            420                 425                 430         


Ser Pro Gly His Arg Lys Thr Pro Phe Thr Lys Asp Lys His Ser Ser 
        435                 440                 445             


Arg Leu Glu Ala His Leu Thr Arg Asp Glu Leu Arg Ala Lys Ala Leu 
    450                 455                 460                 


His Ile Pro Phe Pro Val Glu Lys Ile Ile Asn Leu Pro Val Val Asp 
465                 470                 475                 480 


Phe Asn Glu Met Met Ser Lys Glu Gln Phe Asn Glu Ala Gln Leu Ala 
                485                 490                 495     


Leu Ile Arg Asp Ile Arg Arg Arg Gly Lys Asn Lys Val Ala Ala Gln 
            500                 505                 510         


Asn Cys Arg Lys Arg Lys Leu Glu Asn Ile Val Glu Leu Glu Gln Asp 
        515                 520                 525             


Leu Asp His Leu Lys Asp Glu Lys Glu Lys Leu Leu Lys Glu Lys Gly 
    530                 535                 540                 


Glu Asn Asp Lys Ser Leu His Leu Leu Lys Lys Gln Leu Ser Thr Leu 
545                 550                 555                 560 


Tyr Leu Glu Val Phe Ser Met Leu Arg Asp Glu Asp Gly Lys Pro Tyr 
                565                 570                 575     


Ser Pro Ser Glu Tyr Ser Leu Gln Gln Thr Arg Asp Gly Asn Val Phe 
            580                 585                 590         


Leu Val Pro Lys Ser Lys Lys Pro Asp Val Lys Lys Asn 
        595                 600                 605 


<210>  9
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  9
tcgatgtgac cgggaatatc agg                                               23


<210>  10
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  10
tgatttagac ggtatgcaac agg                                               23


<210>  11
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  11
aagtacaaag catctgattt ggg                                               23


<210>  12
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  12
agcatctgat ttgggaatgt ggg                                               23


<210>  13
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  13
tggatttgat tgacatactt tgg                                               23


<210>  14
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  14
gcttcttact tttgggaaca agg                                               23


<210>  15
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  15
tttgattgac atactttgga ggcaa                                             25


<210>  16
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  16
ttttccttgt tcccaaaagt aagaa                                             25


<210>  17
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  17
caagatatag atcttggagt aag                                               23


<210>  18
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  18
aagatataga tcttggagta ag                                                22


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  19
gatatagatc ttggagtaag                                                   20


<210>  20
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  20
tatagatctt ggagtaag                                                     18


<210>  21
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  21
atagatcttg gagtaag                                                      17


<210>  22
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  22
tggaggcaag atatagatct tgg                                               23


<210>  23
<211>  200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ssDNA template

<400>  23
ttaaaaaaca tgagctctct ccttcctttt tttgtcttaa acataggaca tggatttgat       60

tgacatactt tggaggcaag atatagatct tggagtaagt ggagaagtat ttgacttcag      120

tcagcgacgg aaagagtatg agctggaaaa acagaaaaaa cttgaaaagg aaagacaaga      180

acaactccaa aaggagcaag                                                  200


<210>  24
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  24
tcgatgtgac cgggaatatc agg                                               23


<210>  25
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  25
tgatttagac ggtatgcaac agg                                               23


