                         SEQUENCE LISTING

<110>  Liu, Shixin
       Ju, Xiangwu
 
<120>  Methods of Full-Length RNA Profiling

<130>  1119-63 PCT

<140>  Unassigned
<141>  2020-05-22

<150>  US 62/851,969
<151>  2019-05-23

<160>  56    

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' adaptor


<220>
<221>  misc_feature
<222>  (17)..(22)
<223>  n can be any nucleotide

<400>  1
cccaaacucu ccacgunnnn nn                                                22


<210>  2
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' adaptor


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' phosphorylation

<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  dideoxycytidine

<400>  2
nnnnaaccug cuaucaacug c                                                 21


<210>  3
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' adaptor


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' phosphorylation

<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  dideoxycytidine

<400>  3
nnnngcuucc ugcuaucaac ugc                                               23


<210>  4
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' adaptor


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' phosphorylation

<220>
<221>  misc_feature
<222>  (2)..(6)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  dideoxycytidine

<400>  4
nnnnnnauag caaccugcua ucaacugc                                          28


<210>  5
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Biotinylated RT primer


<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  internal biotin dT

<400>  5
gggcagttga tagcagg                                                      17


<210>  6
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7A2 half-1 1878781 forward

<400>  6
gctaggtaac agacgcaatc aatgggtgcg gtattatttt tccggttgta atc              53


<210>  7
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7A2 half-2 1879297 reverse

<400>  7
gctaggtaac atgatgattg tgcgtgctac agcgactaac ccgctaatgc aaaacag          57


<210>  8
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7A2 full-1 forward

<400>  8
tggtcggcgt ttttaaaaca ggtattgaca acatgaagta acatgcagta agatacaaat       60

cgctaggtaa cagacgcaat caatgggtgc                                        90


<210>  9
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7A2 full-2 reverse

<400>  9
cggcgccatt tttaaaacag gtattgacaa catgaagtaa catgcagtaa gatacaaatc       60

gctaggtaac atgatgattg tgcgtgctac                                        90


<210>  10
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7A2 half-1 922200 forward

<400>  10
gctaggtaac agacgcaatc aatgggtgcc tctgctcgac ggtcaccggg                  50


<210>  11
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7A2 half-2 922600 reverse

<400>  11
gctaggtaac atgatgattg tgcgtgctac atgtcgaagc atggaaaagg g                51


<210>  12
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7A2 half-1 1879000 forward

<400>  12
gctaggtaac agacgcaatc aatgggtgct aagcgttaac ttttaatctt c                51


<210>  13
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7A2 half-2 1879300 reverse

<400>  13
gctaggtaac atgatgattg tgcgtgctac atcagcgact aacccgctaa tg               52


<210>  14
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7A2 half-1 1028290 forward

<400>  14
gctaggtaac agacgcaatc aatgggtgcc cgggttaaat gtggtgatgg                  50


<210>  15
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7A2 half-2 1028540 reverse

<400>  15
gctaggtaac atgatgattg tgcgtgctac ggacgataac ggcctaccgg                  50


<210>  16
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7A2 half-1 1863700 reverse

<400>  16
gctaggtaac agacgcaatc aatgggtgcg tggtacgaca acgaaaccgg                  50


<210>  17
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7A2 full-1 2699900 forward

<400>  17
gctaggtaac agacgcaatc aatgggtgca ccacgcggat aaaatttaat attc             54


<210>  18
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7A2 full-2 2700210 reverse

<400>  18
gctaggtaac atgatgattg tgcgtgctac cgaatgactg attgccgata cc               52


<210>  19
<211>  51
<212>  DNA
<213>  Artificial Sequience

<220>
<223>  T7A2 full-1 1524276 forward

<400>  19
gctaggtaac agacgcaatc aatgggtgca tggcaaaacg ctgtatgtca g                51


<210>  20
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7A2 full-2 1524600 reverse

<400>  20
gctaggtaac atgatgattg tgcgtgctac ctggttatag gctggtttgg tg               52


<210>  21
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1863950 reverse

<400>  21
ggatgatcga ccacaatgag gtcc                                              24


<210>  22
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spike-in RNA (C gene 500nt) forward

<400>  22
tacgtaatac gactcactat aggggtgaca gcagagctgc gtaatctc                    48


<210>  23
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spike-in RNA (C gene 500nt) reverse

<400>  23
gccgggaggc ggcactggca ag                                                22


<210>  24
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spike-in RNA (B gene 1000nt) forward

<400>  24
tacgtaatac gactcactat agggatgaaa acgcccacca ttcccac                     47


<210>  25
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spike-in RNA (B gene 1000nt) reverse

<400>  25
ccgtatcctg agccgtctgc ag                                                22


<210>  26
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spike-in RNA (A gene 1500nt) forward

<400>  26
tacgtaatac gactcactat aggggtgaat atatcgaaca gtcagg                      46


<210>  27
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spike-in RNA (A gene 1500nt) reverse

<400>  27
ggccaccggc tttccgtaga cgg                                               23


<210>  28
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spike-in RNA (H gene 2000nt) forward

<400>  28
tacgtaatac gactcactat agggatggct gaaccggtag gcgatctg                    48


<210>  29
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spike-in RNA (H gene 2000nt) reverse

<400>  29
tcatcatgga cagcacggaa cggg                                              24


<210>  30
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TR kana stop site forward

<400>  30
gcatacataa aaaacctgcc agcgatggca ggtttttttt attccgggga tccgtcgacc       60


<210>  31
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TR kana stop site reverse

<400>  31
aaccacgttt aaaaaaggtg ctcaatgagc accttttttc tgtaggctgg agctgcttcg       60


<210>  32
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  yoak PD_stop forward

<400>  32
tttcgtcgct gcgagaacgc attcagcagc aaatacgtgg gcatacataa aaaacctgcc       60


<210>  33
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  yoak PD_stop reverse

<400>  33
ctttatgctc ccggggcagc atagccgcca ataaaaaacc aaccacgttt aaaaaaggtg       60


<210>  34
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ynaj PD_stop forward

<400>  34
atcgaataca tgaaagccag catccgggcc agggtggagc gcatacataa aaaacctgcc       60


<210>  35
<211>  60
<212>  DNA
<213>  ARtificial Sequence

<220>
<223>  ynaj PD_stop reverse

<400>  35
aaatttcttc cctttcgctg acttcagttt cgccataatc aaccacgttt aaaaaaggtg       60


<210>  36
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  uspe PD_stop forward

<400>  36
tcttctggca gacctttttc tacgtgcgtc atgttttcat gcatacataa aaaacctgcc       60


<210>  37
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  

uspe PD_stop reverse

<400>  37
aaatttttct gatttattga tctggcagaa ggttcatcac aaccacgttt aaaaaaggtg       60


<210>  38
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hspq PD_stop forward

<400>  38
ccagctcatc aggcgacggt tcagagagcg aataaaccgg gcatacataa aaaacctgcc       60


<210>  39
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hspq PD_stop reverse

<400>  39
tattgcacgg cagaacgttg agctgaacaa actggatctg aaccacgttt aaaaaaggtg       60


<210>  40
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  yoak PD_S pcr forward

<400>  40
acccatcata ataatcgttg tt                                                22


<210>  41
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  yoak PD_S pcr reverse

<400>  41
ttcattacag cggtcgta                                                     18


<210>  42
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  yoak PD_S RT primer

<400>  42
taatcttcat tacagcg                                                      17


<210>  43
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ynaj PD_S pcr forward

<400>  43
gtaaagacca ggctataaac aa                                                22


<210>  44
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ynaj PD_S pcr reverse

<400>  44
tattccgctg tctgagtg                                                     18


<210>  45
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ynaj RT primer

<400>  45
aatttctctt tggtttg                                                      17


<210>  46
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  uspe PD_S qpcr forward

<400>  46
tcttcttcgt catccagtt                                                    19


<210>  47
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  uspe PD_S qpcr reverse

<400>  47
cggtatttca gcagcatt                                                     18


<210>  48
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  uspe PD_S RT primer

<400>  48
ggtgattcct gatttgg                                                      17


<210>  49
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hspq PD_S qpcr forward

<400>  49
atggctgttc aggatgtt                                                     18


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hspq PD_S qpcr reverse

<400>  50
gtagtgatgg aggacgataa                                                   20


<210>  51
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hspq PD_S RT primer

<400>  51
cggtgaatga cgagcttc                                                     18


<210>  52
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pnp del forward

<400>  52
ccccccgccg cagcggaggg caaatggcaa ccttactcgc attccgggga tccgtcgacc       60


<210>  53
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pnp del reverse

<400>  53
ttcccactga ccgttaacta tcaggagcgt acctacgctg tgtaggctgg agctgcttcg       60


<210>  54
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rnr del forward

<400>  54
atcctttcca ggaacgcgaa gctgaaaaat acgcgaatcc attccgggga tccgtcgacc       60


<210>  55
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rnr del reverse

<400>  55
cctcttcttt taaagagggt attgatcact ctgccacttt tgtaggctgg agctgcttcg       60


<210>  56
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rnb del forward

<400>  56
cgcgaccggg cgcgcaataa tgctgcgggt ttccatgcgg attccgggga tccgtcgacc       60


