                         SEQUENCE LISTING

<110>  KENT STATE UNIVERSITY
 
<120>  ANIMAL CELL LINES FOR FOODS CONTAINING CULTURED ANIMAL CELLS

<130>  KENT-BA-PROV-PCT

<150>  US 62/850,803
<151>  2019-05-21

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  24
<212>  DNA
<213>  Bovine GAPDH Forward primer

<400>  1
tcccaacgtg tctgttgtgg atct                                              24


<210>  2
<211>  24
<212>  DNA
<213>  Bovine GAPDH Reverse primer

<400>  2
tgttgaagtc gcaggagaca acct                                              24


<210>  3
<211>  20
<212>  DNA
<213>  Bovine Myoglobin Forward primer

<400>  3
tctgcatggt acctggcctc                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Bovine Myoglobin Reverse primer

<400>  4
caagtggaga gcctagcgtg                                                   20


<210>  5
<211>  461
<212>  DNA
<213>  Bovine Myoglobin

<400>  5
atggggctca gcgacgggga atggcagttg gtgctgaatg cctgggggaa ggtggaggct       60

gatgtcgcag gccatgggca ggaggtcctc atcaggctct tcacaggtca tcccgagacc      120

ctggagaaat ttgacaagtt caagcacctg aagacagagg ctgagatgaa ggcctccgag      180

gacctgaaga agcatggcaa cacggtgctc acggccctgg ggggtatcct gaagaaaaag      240

ggtcaccatg aggcagaggt gaagcacctg gccgagtcac atgccaacaa gcacaagatc      300

cctgtcaagt acctggagtt catctcggac gccatcatcc atgttctaca tgccaagcat      360

ccttcaactt cggtgctgat gcccaggctg ccatgagcaa ggccctggaa ctgttccgga      420

atgacatggc tgcccagtac aaggtgctgg gcttccatgg c                          461


<210>  6
<211>  24
<212>  DNA
<213>  Bovine Myogenin forward primer

<400>  6
agcctccaaa tccactccct gaaa                                              24


<210>  7
<211>  24
<212>  DNA
<213>  Bovine Myogenin reverse primer

<400>  7
agccactggc ataggaagag atga                                              24


<210>  8
<211>  900
<212>  DNA
<213>  Bovine BMI-1 promoter

<400>  8
gccgtgccgg cccctccccc gtgcccgccg ccgccgccgc cgccgccgcc gcccggcagc       60

cccgcacgcc cgccgaagct ccgggctcgg ccgggctccg cgcgcggagt tgcagcggtg      120

gccggatgcc aagtgtaagt gtaagttgct atggaaaccc cgacagaggc aagttccgaa      180

tccggagcga gacggagccc cgggcgccgc cggatccgcc cctcgcatcc cggcccccgg      240

gcgtccgcgc gctcaggccc cagcccgagg ccgactcgag gtgcttctcc tgcggcccga      300

gcacccagct ccggaaatgc cagggatgca gatagagcag aatttgcttt ccctttgtat      360

cacgtcaaaa cgtgccaggt tctggtggct ggaaccgcct aaaacaaccg gaacccctgg      420

gaagcggggg catgctcctg gattttcgat cgaagagccg taaggaagtt ttacgataaa      480

tttggagtcc tggaacaacc cctcgcggtt ggtaaatatc tgcggggagt gtgtggcgtc      540

tgcagcagcc gtggggctgc tgggctggag gacaaatgga agaaagcgac ccgaatactc      600

tcagctccca gcccccacct cagacctttt cttctccctc ctggaatgac ctgagggacc      660

agatattact tttttggggt tctttttcat cttttcagta gaattgatcg aggtccgatc      720

ggtgaattcc ttatgtagaa gatgttggga caatcctgct gcagtgttaa aaatgcattt      780

tatgaactcc tccaacatat cagagcgtat ggttcctggg agttggagat aatctcaatt      840

ctctttctgt gaattatagc cagtattact ttgtcttgca ggatctttta tcaagcagaa      900


<210>  9
<211>  981
<212>  DNA
<213>  Bovine BMI-1 coding sequence

<400>  9
atgcatcgaa caaccagaat caagatcact gagctaaatc cccacctaat gtgtgttctt       60

tgtggagggt acttcattga tgccacaacc ataatagaat gtctacattc cttctgtaaa      120

acgtgtattg tgcgttacct ggagaccagc aagtattgtc ctatctgtga tgtccaagtt      180

cacaaaacca gaccactact gaatataagg tcagataaaa ctcttcaaga tattgtatac      240

aaattagttc cagggctttt caaaaatgaa atgaagagaa gaagggattt ttatgccgct      300

catccttcag ctgatgctgc caatggctct aatgaagaca gaggagaagt ggctgatgaa      360

gataagagaa ttataactga tgatgagata ataagtttat ccattgaatt ctttgaccag      420

aacagattgg atcggaaaat aaacaaggac aaagagaaat ctaaggagga ggtgaatgat      480

aaaagatatt tacgatgccc agcagcaatg actgtaatgc acctaagaaa gtttctcaga      540

agtaaaatgg acatacctaa tactttccag attgatgtca tgtatgaaga ggaaccttta      600

aaagattact atacactaat ggatattgcc tacatttata cctggagaag gaatggccca      660

cttcctttga aatacagagt tcgacctact tgtaaaagaa tgaagatcag tcatcagaga      720

gatggactga ctaacactgg agaactggaa agtgactctg ggagtgacaa ggccaacagc      780

ccagcaggag gcatcccctc cacctcttcc tgtttgccca gtcccagcac tccagtccag      840

tctcctcatc ctcagtttcc tcacatttcc agtactatga atggaaccag cagcagcccc      900

agcggtaacc accaatcttc ctttgccaat agacctcgaa aatcatcagt aaatgggtcg      960

tcagcaactt catctggttg a                                                981


<210>  10
<211>  34
<212>  DNA
<213>  FRT

<400>  10
gaagttccta ttctctagaa agtataggaa cttc                                   34


<210>  11
<211>  465
<212>  DNA
<213>  Bovine myoglobin coding sequence with last amine acid

<400>  11
atggggctca gcgacgggga atggcagttg gtgctgaatg cctgggggaa ggtggaggct       60

gatgtcgcag gccatgggca ggaggtcctc atcaggctct tcacaggtca tcccgagacc      120

ctggagaaat ttgacaagtt caagcacctg aagacagagg ctgagatgaa ggcctccgag      180

gacctgaaga agcatggcaa cacggtgctc acggccctgg ggggtatcct gaagaaaaag      240

ggtcaccatg aggcagaggt gaagcacctg gccgagtcac atgccaacaa gcacaagatc      300

cctgtcaagt acctggagtt catctcggac gccatcatcc atgttctaca tgccaagcat      360

ccttcagact tcggtgctga tgcccaggct gccatgagca aggccctgga actgttccgg      420

aatgacatgg ctgcccagta caaggtgctg ggcttccatg gctaa                      465


<210>  12
<211>  771
<212>  DNA
<213>  Bovine myoglobin promoter

<400>  12
gaggtaagca gtgtgacagg aacacatgcg aataggtgga aagggcaggc agttaattgt       60

gccttgaggg ggcacatgac gcacaatttt tcagaggaaa atatctgaac aatatttgag      120

ctttctgggt ggagtgggaa aatgcaggct ccaagagggt atggatctgc ctgggttcac      180

ccagttataa gcaggaaacc atcgaggttc cttcccacca ctctgaaaag tgagaggcat      240

tctggcaaag tgggcttcta gacggtgggc aaagagactg ctaaggccag gacagtccca      300

gggccaagcc agggtgcctg ctgccctggg cttagagata tgacaggtcc tcttggggtg      360

gctgacagca gggggagttg ggtttcaggc cactggcgtc agccctagcc ttgccctttc      420

tgttggcctc tgagagtcca aacagtggcc cagcctcctc cccactctcc gcacacacaa      480

ccccaccacc accacacccg tgacctgagt tggcctacct ccccacaatg gcacctgcct      540

caaaatagct tccatgtgag ggctagagaa aggaaaagat tagaccccta catgagagag      600

gggggtgggg aggagggaga gagagagtga gtgagctgtc aagtgatccc tgttaagcat      660

ctgggaaggt ataaaatccc tctggggcca ggcagcctca aaccccagct gtcggagaca      720

ggacacccag tcagtccgcc cttgttcttt ttctcttctt cagactgcgc c               771


<210>  13
<211>  548
<212>  DNA
<213>  Bovine myoglobin 3' UTR and putative terminator

<400>  13
gccccacccc tgtgcccctc accccaccca cctgggcagg gtgggcgggg actgaatccc       60

aagtagttat agggtttgct tctgagtgtg tgctttgttt aggagaggtg ggtggaagag      120

gtggatgggt taggggtgga gggagccttg ggagaggcct ggggaccagg ctttcagtgg      180

agggtcatca acttgggaac catgagaagc ttgactgtgg ctggctgagt ctgggtcaaa      240

ctcaactttc ctttcacctc aatgccaacc caattcctac caacctctaa actgacctgc      300

acctttaccc tcaccttaaa tccccaatcc gagctgtcaa cataaactcc agcctaattc      360

tctgacccca tcacccagcc ccttgaagac agcagagtgt cttgcttgcc ctgagaagga      420

agtgtgggcc gggtgggacg gccacaccca gccctaggga ggcatggagg catggtgtct      480

gcaacataaa tgtcccttct caggtagggg agtgacacct ggtttaataa aggatttctc      540

acatcaca                                                               548


<210>  14
<211>  1580
<212>  DNA
<213>  Tet transactivator

<400>  14
tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat       60

agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg      120

cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata      180

gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta      240

catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc      300

gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac      360

gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga      420

tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg      480

ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg      540

caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac      600

cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac      660

cgatccagcc tccgcggccc cgaattcacc atgtctagac tggacaagag caaagtcata      720

aactctgctc tggaattact caatggagtc ggtatcgaag gcctgacgac aaggaaactc      780

gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc      840

ctgctcgatg ccctgccaat cgagatgctg gacaggcatc atacccactc ctgccccctg      900

gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc      960

ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac     1020

gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca     1080

ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag     1140

catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg     1200

aaacaagcaa ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg     1260

gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac     1320

gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt     1380

gatatgctgc ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaacta     1440

agtaaggatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg     1500

cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt     1560

ataagctgca ataaacaagt                                                 1580


<210>  15
<211>  1028
<212>  DNA
<213>  Tet inducible promoter

<400>  15
tcctcgagtt tactccctat cagtgataga gaacgtatga agagtttact ccctatcagt       60

gatagagaac gtatgcagac tttactccct atcagtgata gagaacgtat aaggagttta      120

ctccctatca gtgatagaga acgtatgacc agtttactcc ctatcagtga tagagaacgt      180

atctacagtt tactccctat cagtgataga gaacgtatat ccagtttact ccctatcagt      240

gatagagaac gtataagctt taggcgtgta cggtgggcgc ctataaaagc agagctcgtt      300

tagtgaaccg tcagatcgcc tggagcaatt ccacaacact tttgtcttat accaactttc      360

cgtaccactt cctaccctcg taaagtcgac accggggccc agatctatcg atcggccggc      420

ccctctccct cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg      480

tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg      540

gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg      600

aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca      660

aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct      720

ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca      780

cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa      840

ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggta      900

cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg      960

ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccggg ccggatatca     1020

cgcgtcat                                                              1028


<210>  16
<211>  1498
<212>  DNA
<213>  FLP with poly A

<400>  16
atgccacaat ttggtatatt atgtaaaaca ccacctaagg tgcttgttcg tcagtttgtg       60

gaaaggtttg aaagaccttc aggtgagaaa atagcattat gtgctgctga actaacctat      120

ttatgttgga tgattacaca taacggaaca gcaatcaaga gagccacatt catgagctat      180

aatactatca taagcaattc gctgagtttc gatattgtca ataaatcact ccagtttaaa      240

tacaagacgc aaaaagcaac aattctggaa gcctcattaa agaaattgat tcctgcttgg      300

gaatttacaa ttattcctta ctatggacaa aaacatcaat ctgatatcac tgatattgta      360

agtagtttgc aattacagtt cgaatcatcg gaagaagcag ataagggaaa tagccacagt      420

aaaaaaatgc ttaaagcact tctaagtgag ggtgaaagca tctgggagat cactgagaaa      480

atactaaatt cgtttgagta tacttcgaga tttacaaaaa caaaaacttt ataccaattc      540

ctcttcctag ctactttcat caattgtgga agattcagcg atattaagaa cgttgatccg      600

aaatcattta aattagtcca aaataagtat ctgggagtaa taatccagtg tttagtgaca      660

gagacaaaga caagcgttag taggcacata tacttcttta gcgcaagggg taggatcgat      720

ccacttgtat atttggatga atttttgagg aattctgaac cagtcctaaa acgagtaaat      780

aggaccggca attcttcaag caataaacag gaataccaat tattaaaaga taacttagtc      840

agatcgtaca ataaagcttt gaagaaaaat gcgccttatt caatctttgc tataaaaaat      900

ggcccaaaat ctctcattgg aagacatttg atgacctcat ttctttcaat gaagggccta      960

acggagttga ctaatgttgt gggaaattgg agcgataagc gtgcttctgc cgtggccagg     1020

acaacgtata ctcatcagat aacagcaata cctgatcact acttcgcact agtttctcgg     1080

tactatgcat atgatccaat atcaaaggaa atgatagcat tgaaggatga gactaatcca     1140

attgaggagt ggcagcatat agaacagcta aagggtagtg ctgaaggaag catacgatac     1200

cccgcatgga atgggataat atcacaggag gtactagact acctttcatc ctacataaat     1260

agacgcatat aagtacgcat ttaagcataa acacgcacta tgccgttctt ctcatgtata     1320

tatatataca ggcaacacgc agatataggt gcgacgtgaa cagtgagctg tatgtgcgca     1380

gctcgcgttg cattttcgga agcgctcgtt ttcggaaacg ctttgaagtt cctattccga     1440

agttcctatt ctctagttct agagcggccg ccaccgcggt ggagctccag cttttgtt       1498


