                         SEQUENCE LISTING

<110>  Nanjing Agricultural University
 
<120>  Methods for improving rice grain yield

<130>  PC928133WOA

<150>  PCT/CN2019/088133
<151>  2019-05-23

<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  242
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NRT2.3 (partial) promoter

<400>  1
gacgcgagcg cggagacggc agcgccggcc tcccaccggt cgcgtaagat cacgcccgaa       60

atctttattc attttctctc caccggttgc cctctcgccg cacccaacca tcgcgccacg      120

ccgcgccgcg ctgccggagc cgcgctttcc gctatgctat aagagctgac gcgcagggca      180

cagcggatgt acgtacacac agtcactagc taagctgcta gccttgctac cacgtgttgg      240

ag                                                                     242


<210>  2
<211>  242
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NRT2.3 promoter with polymorphism at -81 upstream of ATG.

<400>  2
gacgcgagcg cggagacggc agcgccggcc tcccaccggt cgcgtaagat cacgcccgaa       60

atctttattc attttctctc caccggttgc cctctcgccg cacccaacca tcgcgccacg      120

ccgcgccgcg ctgccggagc cgcgctttcc gctatgctac aagagctgac gcgcagggca      180

cagcggatgt acgtacacac agtcactagc taagctgcta gccttgctac cacgtgttgg      240

ag                                                                     242


<210>  3
<211>  242
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NRT2.3 promoter with polymorphism at -81, -42 and -21 upstream of
       ATG.

<400>  3
gacgcgagcg cggagacggc agcgccggcc tcccaccggt cgcgtaagat cacgcccgaa       60

atctttattc attttctctc caccggttgc cctctcgccg cacccaacca tcgcgccacg      120

ccgcgccgcg ctgccggagc cgcgctttcc gctatgctac aagagctgac gcgcagggca      180

cagcggatgt acgtacacac cgtcactagc taagctgcta gtcttgctac cacgtgttgg      240

ag                                                                     242


<210>  4
<211>  180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  180 C-terminal residues of the NRT2.3 promoter

<400>  4
ctttattcat tttctctcca ccggttgccc tctcgccgca cccaaccatc gcgccacgcc       60

gcgccgcgct gccggagccg cgctttccgc tatgctacaa gagctgacgc gcagggcaca      120

gcggatgtac gtacacaccg tcactagcta agctgctagt cttgctacca cgtgttggag      180


<210>  5
<211>  141
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  141 C-terminal residues of the NRT 2.3 promoter

<400>  5
acccaaccat cgcgccacgc cgcgccgcgc tgccggagcc gcgctttccg ctatgctaca       60

agagctgacg cgcagggcac agcggatgta cgtacacacc gtcactagct aagctgctag      120

tcttgctacc acgtgttgga g                                                141


<210>  6
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  deletion of 62 5' nucleotides of the NRT2.3 promoter

<400>  6
gacgcgagcg cggagacggc agcgccggcc tcccaccggt cgcgtaagat cacgcccgaa       60

at                                                                      62


<210>  7
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  deletion of the 101 5' nucleotides of the NRT2.3 promoter

<400>  7
gacgcgagcg cggagacggc agcgccggcc tcccaccggt cgcgtaagat cacgcccgaa       60

atctttattc attttctctc caccggttgc cctctcgccg c                          101


<210>  8
<211>  2074
<212>  DNA
<213>  Oryza sativa

<400>  8
gagcgccggc ctcccaccgg tcgcgtaaga tcacgcccga aatctttatt cattttctct       60

ccaccggttg ccctctcgcc gcacccaacc atcgcgccac gccgcgccgc gctgccggag      120

ccgcgctttc cgctatgcta taagagctga cgcgcagggc acagcggatg tacgtacaca      180

cagtcactag ctaagctgct agccttgcta ccacgtgttg gagatggagg ctaagccggt      240

ggcgatggag gtggaggggg tcgaggcggc ggggggcaag ccgcggttca ggatgccggt      300

ggactccgac ctcaaggcga cggagttctg gctcttctcc ttcgcgaggc cacacatggc      360

ctccttccac atggcgtggt tctccttctt ctgctgcttc gtgtccacgt tcgccgcgcc      420

gccgctgctg ccgctcatcc gcgacaccct cgggctcacg gccacggaca tcggcaacgc      480

cgggatcgcg tccgtgtcgg gcgccgtgtt cgcgcgtctg gccatgggca cggcgtgcga      540

cctggtcggg cccaggctgg cctccgcgtc tctgatcctc ctcaccacac cggcggtgta      600

ctgctcctcc atcatccagt ccccgtcggg gtacctcctc gtgcgcttct tcacgggcat      660

ctcgctggcg tcgttcgtgt cggcgcagtt ctggatgagc tccatgttct cggcccccaa      720

agtggggctg gccaacggcg tggccggcgg ctggggcaac ctcggcggcg gcgccgtcca      780

gctgctcatg ccgctcgtgt acgaggccat ccacaagatc ggtagcacgc cgttcacggc      840

gtggcgcatc gccttcttca tcccgggcct gatgcagacg ttctcggcca tcgccgtgct      900

ggcgttcggg caggacatgc ccggcggcaa ctacgggaag ctccacaaga ctggcgacat      960

gcacaaggac agcttcggca acgtgctgcg ccacgccctc accaactacc gcggctggat     1020

cctggcgctc acctacggct acagcttcgg cgtcgagctc accatcgaca acgtcgtgca     1080

ccagtacttc tacgaccgct tcgacgtcaa cctccagacc gccgggctca tcgccgccag     1140

cttcgggatg gccaacatca tctcccgccc cggcggcggg ctactctccg actggctctc     1200

cagccggtac ggcatgcgcg gcaggctgtg ggggctgtgg actgtgcaga ccatcggcgg     1260

cgtcctctgc gtggtgctcg gaatcgtcga cttctccttc gccgcgtccg tcgccgtgat     1320

ggtgctcttc tccttcttcg tccaggccgc gtgcgggctc accttcggca tcgtgccgtt     1380

cgtgtcgcgg aggtcgctgg ggctcatctc cgggatgacc ggcggcgggg gcaacgtggg     1440

cgccgtgctg acgcagtaca tcttcttcca cggcacaaag tacaagacgg agaccgggat     1500

caagtacatg gggctcatga tcatcgcgtg cacgctgccc gtcatgctca tctacttccc     1560

gcagtggggc ggcatgctcg taggcccgag gaagggggcc acggcggagg agtactacag     1620

ccgggagtgg tcggatcacg agcgcgagaa gggtttcaac gcggccagcg tgcggttcgc     1680

ggagaacagc gtgcgcgagg gcgggaggtc gtcggcgaat ggcggacagc ccaggcacac     1740

cgtccccgtc gacgcgtcgc cggccggggt gtgaagaatg ccacggacaa taaggtcgcg     1800

gttgtagtac aactgtacaa attgatggta cgtgtcgttt gaccgcgcgc gcgcacagtg     1860

tgggtcgtgg cctcgtgggc ttagtggagt acagtgaggg gtgtacgtgt gtcgtggcgc     1920

gcgcggtcac ctcggtggcc ttgggattgg gggggcacta tacgctagta ctccagatat     1980

atacgggttt gatttacttc tgtggatcgg cgcttgttgg tggtttgctc cctgtggttt     2040

ttgtgatggt aatcatactc atactcaaac agtc                                 2074


<210>  9
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NRT2.3 TATA box

<400>  9
ccgctatgct ataagagctg ac                                                22


<210>  10
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OSE2ROOTNODULE

<400>  000



<210>  11
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ASF1MOTIFCAMV

<400>  000



<210>  12
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR target sequence

<400>  12
gctataagag ctgacgcgca ggg                                               23


<210>  13
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR target sequence

<400>  13
ccggagccgc gctttccgct atg                                               23


<210>  14
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR target sequence

<400>  14
ccggttgccc tctcgccgca ccc                                               23


<210>  15
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR target sequence

<400>  15
ccgcgacgcg agcgcggaga cgg                                               23


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  protospacer sequence

<400>  16
gctataagag ctgacgcgca                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  protospacer sequence

<400>  17
gagccgcgct ttccgctatg                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  protospacer sequence

<400>  18
gttgccctct cgccgcaccc                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  protospacer sequence

<400>  19
cgacgcgagc gcggagacgg                                                   20


<210>  20
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  protospacer sequence

<400>  20
ggcagctata agagctgacg cgca                                              24


<210>  21
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  protospacer sequence

<400>  21
ggcacatagc ggaaagcgcg gctc                                              24


<210>  22
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  protospacer sequence

<400>  22
ggcagggtgc ggcgagaggg caac                                              24


<210>  23
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  protospacer sequence

<400>  23
ggcaccgtct ccgcgctcgc gtcg                                              24


<210>  24
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA nucleic acid sequence

<400>  24
atgctactac taaaaaaaag caccgactcg gtgccacttt ttcaagttga taacgactag       60
ccttatttta acttgctatg cttttcagca tagctctaaa ac                         102


<210>  25
<211>  388
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U3 promoter sequence

<400>  25
aaaggaagga atctttaaac atacgaacag atcacttaaa gttcttctga agcaacttaa       60
agttatcagg catgcatgga tcttggagga atcagatgtg cagtcaggga ccatagcaca      120
agacaggcgt cttctactgg tgctaccagc aaatgctgga agccgggaac actgggtacg      180
ttggaaacca cgtgtgatgt gaaggagtaa gataaactgt aggagaaaag catttcgtag      240
tgggccatga agcctttcag gacatgtatt gcagtatggg ccggcccatt acgcaattgg      300
acgacaacaa agactagtat tagtaccacc tcggctatcc acatagatca aagctggttt      360
aaaagagttg tgcagatgat ccgtggca                                         388


<210>  26
<211>  447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U6a promoter sequence

<400>  26
ttttttcctg tagttttccc acaaccattt tttaccatcc gaatgatagg ataggaaaaa       60
tatccaagtg aacagtattc ctataaaatt cccgtaaaaa gcctgcaatc cgaatgagcc      120
ctgaagtctg aactagccgg tcacctgtac aggctatcga gatgccatac aagagacggt      180
agtaggaact aggaagacga tggttgattc gtcaggcgaa atcgtcgtcc tgcagtcgca      240
tctatgggcc tggacggaat aggggaaaaa gttggccgga taggagggaa aggcccaggt      300
gcttacgtgc gaggtaggcc tgggctctca gcacttcgat tcgttggcac cggggtagga      360
tgcaatagag agcaacgttt agtaccacct cgcttagcta gagcaaactg gactgcctta      420
tatgcgcggg tgctggcttg gctgccg                                          447


<210>  27
<211>  333
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U6b promoter sequence

<400>  27
tgcaagaacg aactaagccg gacaaaaaaa aaaggagcac atatacaaac cggttttatt       60

catgaatggt cacgatggat gatggggctc agacttgagc tacgaggccg caggcgagag      120

aagcctagtg tgctctctgc ttgtttgggc cgtaacggag gatacggccg acgagcgtgt      180

actaccgcgc gggatgccgc tgggcgctgc gggggccgtt ggatggggat cggtgggtcg      240

cgggagcgtt gaggggagac aggtttagta ccacctcgcc taccgaacaa tgaagaaccc      300

accttataac cccgcgcgct gccgcttgtg ttg                                   333


<210>  28
<211>  325
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U3b in dicot plant

<400>  28
tttactttaa attttttctt atgcagcctg tgatggataa ctgaatcaaa caaatggcgt       60

ctgggtttaa gaagatctgt tttggctatg ttggacgaaa caagtgaact tttaggatca      120

acttcagttt atatatggag cttatatcga gcaataagat aagtgggctt tttatgtaat      180

ttaatgggct atcgtccata gattcactaa tacccatgcc cagtacccat gtatgcgttt      240

catataagct cctaatttct cccacatcgc tcaaatctaa acaaatcttg ttgtatatat      300

aacactgagg gagcaacatt ggtca                                            325


<210>  29
<211>  305
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U6-1 in dicot plant

<400>  29
agaaatctca aaattccggc agaacaattt tgaatctcga tccgtagaaa cgagacggtc       60

attgttttag ttccaccacg attatatttg aaatttacgt gagtgtgagt gagacttgca      120

taagaaaata aaatctttag ttgggaaaaa attcaataat ataaatgggc ttgagaagga      180

agcgagggat aggccttttt ctaaaatagg cccatttaag ctattaacaa tcttcaaaag      240

taccacagcg cttaggtaaa gaaagcagct gagtttatat atggttagag acgaagtagt      300

gattg                                                                  305


<210>  30
<211>  4131
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  30
atggccccaa agaagaagcg caaggtcgac aagaagtact ccatcggcct cgacatcggc       60

accaattctg ttggctgggc cgtgatcacc gacgagtaca aggtgccgtc caagaagttc      120

aaggtcctcg gcaacaccga ccgccactcc atcaagaaga atctcatcgg cgccctgctg      180

ttcgactctg gcgagacagc cgaggctaca aggctcaaga ggaccgctag acgcaggtac      240

accaggcgca agaaccgcat ctgctacctc caagagatct tctccaacga gatggccaag      300

gtggacgaca gcttcttcca caggctcgag gagagcttcc tcgtcgagga ggacaagaag      360

cacgagcgcc atccgatctt cggcaacatc gtggatgagg tggcctacca cgagaagtac      420

ccgaccatct accacctccg caagaagctc gtcgactcca ccgataaggc cgacctcagg      480

ctcatctacc tcgccctcgc ccacatgatc aagttcaggg gccacttcct catcgagggc      540

gacctcaacc cggacaactc cgatgtggac aagctgttca tccagctcgt gcagacctac      600

aaccagctgt tcgaggagaa cccgatcaac gcctctggcg ttgacgccaa ggctattctc      660

tctgccaggc tctctaagtc ccgcaggctc gagaatctga tcgcccaact tccgggcgag      720

aagaagaatg gcctcttcgg caacctgatc gccctctctc ttggcctcac cccgaacttc      780

aagtccaact tcgacctcgc cgaggacgcc aagctccagc tttccaagga cacctacgac      840

gacgacctcg acaatctcct cgcccagatt ggcgatcagt acgccgatct gttcctcgcc      900

gccaagaatc tctccgacgc catcctcctc agcgacatcc tcagggtgaa caccgagatc      960

accaaggccc cactctccgc ctccatgatc aagaggtacg acgagcacca ccaggacctc     1020

acactcctca aggccctcgt gagacagcag ctcccagaga agtacaagga gatcttcttc     1080

gaccagtcca agaacggcta cgccggctac atcgatggcg gcgcttctca agaggagttc     1140

tacaagttca tcaagccgat cctcgagaag atggacggca ccgaggagct gctcgtgaag     1200

ctcaatagag aggacctcct ccgcaagcag cgcaccttcg ataatggctc catcccgcac     1260

cagatccacc tcggcgagct tcatgctatc ctccgcaggc aagaggactt ctacccgttc     1320

ctcaaggaca accgcgagaa gattgagaag atcctcacct tccgcatccc gtactacgtg     1380

ggcccgctcg ccaggggcaa ctccaggttc gcctggatga ccagaaagtc cgaggagaca     1440

atcaccccct ggaacttcga ggaggtggtg gataagggcg cctctgccca gtctttcatc     1500

gagcgcatga ccaacttcga caagaacctc ccgaacgaga aggtgctccc gaagcactca     1560

ctcctctacg agtacttcac cgtgtacaac gagctgacca aggtgaagta cgtgaccgag     1620

gggatgagga agccagcttt ccttagcggc gagcaaaaga aggccatcgt cgacctgctg     1680

ttcaagacca accgcaaggt gaccgtgaag cagctcaagg aggactactt caagaaaatc     1740

gagtgcttcg actccgtcga gatctccggc gtcgaggata ggttcaatgc ctccctcggg     1800

acctaccacg acctcctcaa gattatcaag gacaaggact tcctcgacaa cgaggagaac     1860

gaggacatcc tcgaggacat cgtgctcacc ctcaccctct tcgaggaccg cgagatgatc     1920

gaggagcgcc tcaagacata cgcccacctc ttcgacgaca aggtgatgaa gcagctgaag     1980

cgcaggcgct ataccggctg gggcaggctc tctaggaagc tcatcaacgg catccgcgac     2040

aagcagtccg gcaagacgat cctcgacttc ctcaagtccg acggcttcgc caaccgcaac     2100

ttcatgcagc tcatccacga cgactccctc accttcaagg aggacatcca aaaggcccag     2160

gtgtccggcc aaggcgattc cctccatgag catatcgcca atctcgccgg ctccccggct     2220

atcaagaagg gcattctcca gaccgtgaag gtggtggacg agctggtgaa ggtgatgggc     2280

aggcacaagc cagagaacat cgtgatcgag atggcccgcg agaaccagac cacacagaag     2340

ggccaaaaga actcccgcga gcgcatgaag aggatcgagg agggcattaa ggagctgggc     2400

tcccagatcc tcaaggagca cccagtcgag aacacccagc tccagaacga gaagctctac     2460

ctctactacc tccagaacgg ccgcgacatg tacgtggacc aagagctgga catcaaccgc     2520

ctctccgact acgacgtgga ccatattgtg ccgcagtcct tcctgaagga cgactccatc     2580

gacaacaagg tgctcacccg ctccgacaag aacaggggca agtccgataa cgtgccgtcc     2640

gaagaggtcg tcaagaagat gaagaactac tggcgccagc tcctcaacgc caagctcatc     2700

acccagagga agttcgacaa cctcaccaag gccgagagag gcggcctttc cgagcttgat     2760

aaggccggct tcatcaagcg ccagctcgtc gagacacgcc agatcacaaa gcacgtggcc     2820

cagatcctcg actcccgcat gaacaccaag tacgacgaga acgacaagct catccgcgag     2880

gtgaaggtca tcaccctcaa gtccaagctc gtgtccgact tccgcaagga cttccagttc     2940

tacaaggtgc gcgagatcaa caactaccac cacgcccacg acgcctacct caatgccgtg     3000

gtgggcacag ccctcatcaa gaagtaccca aagctcgagt ccgagttcgt gtacggcgac     3060

tacaaggtgt acgacgtgcg caagatgatc gccaagtccg agcaagagat cggcaaggcg     3120

accgccaagt acttcttcta ctccaacatc atgaatttct tcaagaccga gatcacgctc     3180

gccaacggcg agattaggaa gaggccgctc atcgagacaa acggcgagac aggcgagatc     3240

gtgtgggaca agggcaggga tttcgccaca gtgcgcaagg tgctctccat gccgcaagtg     3300

aacatcgtga agaagaccga ggttcagacc ggcggcttct ccaaggagtc catcctccca     3360

aagcgcaact ccgacaagct gatcgcccgc aagaaggact gggacccgaa gaagtatggc     3420

ggcttcgatt ctccgaccgt ggcctactct gtgctcgtgg ttgccaaggt cgagaagggc     3480

aagagcaaga agctcaagtc cgtcaaggag ctgctgggca tcacgatcat ggagcgcagc     3540

agcttcgaga agaacccaat cgacttcctc gaggccaagg gctacaagga ggtgaagaag     3600

gacctcatca tcaagctccc gaagtacagc ctcttcgagc ttgagaacgg ccgcaagaga     3660

atgctcgcct ctgctggcga gcttcagaag ggcaacgagc ttgctctccc gtccaagtac     3720

gtgaacttcc tctacctcgc ctcccactac gagaagctca agggctcccc agaggacaac     3780

gagcaaaagc agctgttcgt cgagcagcac aagcactacc tcgacgagat catcgagcag     3840

atctccgagt tctccaagcg cgtgatcctc gccgatgcca acctcgataa ggtgctcagc     3900

gcctacaaca agcaccgcga taagccaatt cgcgagcagg ccgagaacat catccacctc     3960

ttcaccctca ccaacctcgg cgctccagcc gccttcaagt acttcgacac caccatcgac     4020

cgcaagcgct acacctctac caaggaggtt ctcgacgcca ccctcatcca ccagtctatc     4080

acaggcctct acgagacacg catcgacctc tcacaactcg gcggcgattg a              4131


<210>  31
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZIIIB

<400>  31
cctgcaggtc gccacattag caatgccaca ttagcaatgc cgactctaga ggatccc          57


<210>  32
<211>  208
<212>  PRT
<213>  Oryza sativa

<400>  32

Met Ala Ala Ala Glu Ala Ala Ala Glu Ala Ala Ala Ala Leu Glu Gly 
1               5                   10                  15      


Ser Glu Pro Val Asp Leu Val Lys His Pro Ser Gly Ile Ile Pro Thr 
            20                  25                  30          


Leu Gln Asn Ile Val Ser Thr Val Asn Leu Asp Cys Lys Leu Asp Leu 
        35                  40                  45              


Lys Ala Ile Ala Leu Gln Ala Arg Asn Ala Glu Tyr Asn Pro Lys Arg 
    50                  55                  60                  


Phe Ala Ala Val Ile Met Arg Ile Arg Glu Pro Lys Thr Thr Ala Leu 
65                  70                  75                  80  


Ile Phe Ala Ser Gly Lys Met Val Cys Thr Gly Ala Lys Ser Glu Gln 
                85                  90                  95      


Gln Ser Lys Leu Ala Ala Arg Lys Tyr Ala Arg Ile Ile Gln Lys Leu 
            100                 105                 110         


Gly Phe Ala Ala Lys Phe Lys Asp Phe Lys Ile Gln Asn Ile Val Gly 
        115                 120                 125             


Ser Cys Asp Val Lys Phe Pro Ile Arg Leu Glu Gly Leu Ala Tyr Ser 
    130                 135                 140                 


His Gly Ala Phe Ser Ser Tyr Glu Pro Glu Leu Phe Pro Gly Leu Ile 
145                 150                 155                 160 


Tyr Arg Met Lys Gln Pro Lys Ile Val Leu Leu Ile Phe Val Ser Gly 
                165                 170                 175     


Lys Ile Val Leu Thr Gly Ala Lys Val Arg Asp Glu Thr Tyr Thr Ala 
            180                 185                 190         


Phe Glu Asn Ile Tyr Pro Val Leu Thr Glu Phe Arg Lys Val Gln Gln 
        195                 200                 205             


<210>  33
<211>  4467
<212>  DNA
<213>  Oryza sativa

<400>  33
gatttccgat cgctatataa aagacctagg atttcgaaat ttttccctcc ccctccccct       60

tcgcgcgcgc gctctcttcc cgcccttttt ttttccttct tcttcaccgg tgggattgat      120

tcgtggggtg cggatctggt ttttgggggt gtgtatggcg gcggcggagg cggcggcgga      180

ggcggcggcg gcgctggagg ggagcgagcc cgtggacctg gtcaagcacc cctccggcat      240

catccccacg ctccagtaag tcccccccgc ccccgcccgg atctgtatgt gcgggttcat      300

cgagctggtt gagtttgagg tggagcggtt aactcgtcgc gcgctgttga tttggttttg      360

ttttgggggc cggggatatt atttgttgtt gcaaatgatg tgattggtgg taggtttagc      420

ttggggggtt actacatgtt tgaggttctg atttgcttga ttgatgaaaa aggggggcga      480

tctgagatcc gcgagtccgg tacgagaaga aatagaggca gccgatgtgc ttgtgctgtg      540

acggatccat tgtccggtct tcataacaac tctttttagc tggacagatg tttgttttct      600

catgaatgaa ttcatatggt tggagtcttg gagaatcagc ttgtcagctg ttcttttttt      660

taaaaaaaaa ttaaggttta taaataaata aataatcaat caatcaatca tgcatctaaa      720

ttgtaggatt taattctttc ccctcatcac gtatttcaac cttagaggga ggtattgtgt      780

ctgtggagag tttatggtgc tcatgtgata catacagttg acaatgggtc aatgcatgtt      840

tttagacagc atcaagctct gaacaagtga accacatatt taggcttgat ccatgtataa      900

ccccactaag ttttatactt gtttgtacgg gccaatatgc ccaaacctgg ctgtttatta      960

gcatgctgta cttgctggtg tgcatcgaaa cgtttttggg gtttgcctac attgctacca     1020

ggcatgtata ttttgcatgc tggtgtgcat tgcagaaaaa gtcgagcatg cttatattgc     1080

tgcttgtcat agtgtacctg cttccatgtg ttctcacaag tgcgcatgcc tgttttgctg     1140

cctttctggg ttgtttatgt ttcagcgttg ggatcatagc tggtagactg catgtgttcc     1200

actctgtttt ggcttatcat gcttcctttg gatactctca ccaaggaaat tgaagtcctc     1260

attatgtgtt ctcatctatt ttgatggcag aaacatcgtg tcgacggtca atttggattg     1320

caaattagac ctcaaagcta tagctttgca agcacgcaat gcagaatata atccaaaggt     1380

attatgatgg ctttggtgtg ttcttgtgtc tttgattttg ctcgaaagga tatcttcttt     1440

gcatgtgaaa attttaccta ttttaatcct gctagcatgt aatatgtaga caagtccata     1500

caatcctatg gtctgttcct aggatgatac ttcctttgtg tataacgcaa gtgtgagtgc     1560

aattttagag gtcggtatgg ataaatgcat agctggcagt ccagttttat attgtaaatc     1620

tcagttaaga gagtgaatac attcatcacg ctcagcttgt cccatggata agcaaattgc     1680

tttacaatta agattctaaa attgctctat ttagtgctta tgcactttct aatctgtgct     1740

gcacgtatca gatacagcat gatgtattgg gggattttgt aattatatgt aatatcataa     1800

tctaaatcta ggctattatg tttattttaa taatggatac attttgaaag aaacagagat     1860

aactgcacac tgaggttata gcatctctat ctactccatc cgttccaaaa tataagcact     1920

gtaatatatg ttttagtttg ctaactcctc ttttcacaaa cagcgttttg ctgcagttat     1980

catgagaata agagaaccga aaactacagc tctgatattt gcatcgggta aaatggtatg     2040

gttcaacctc tttgtatagc taagactgca aatattctgt ttgttcatct tcttattgac     2100

ttgtgaattg ctttatcacc tgtctttgtt gctcttgtag ctattataac gcatattgat     2160

tgatgaactc tttttgctga tgtgtaactg ctttctatta gcctactatc tttttagttt     2220

ttctttgttt ttttaacagc tatcacatcc atacctgatc catgcagcct gtagttcaat     2280

gctgaatctg taaatttaag tttgctctga ttttctgaca tgtttgctgt tgcattgctg     2340

ttacataata acagataagt agtgctcttt ctgtaaatgt atagtttgtc cttgttaact     2400

tttttattgt cttaaagcat aatcagaatt gacaataaat tgaacctgtc atatttattt     2460

gttgatggat aatcagcaat gacacatcac atggacttca tgtcgcgtca atatcttaat     2520

tttaaaaggc ttgtttatga aaattcactt gattttcctt ttggatcaat gtcttgatcc     2580

gaataagctt gttgaaggat aattggtggt cagcattttt gggataaaat cttcattaaa     2640

agttgctttg ctggttctca tcaggaggga taatatctgt gttacagact taaagtaata     2700

catttttctg ataataaatt cacattttaa gcatgagaac gaaggagttc acagcctagg     2760

cttactagta cgctgtatgg ggcctgacta ttaggcagtg gacttaagaa ggggttgtct     2820

tgggtttgat gcaccgattt gctgcgtctt tgcttctttt ttataaaaat caaatttgct     2880

tgttaaccaa aatttctctg tgggcaccat gacctgctag catttgtggt ggaattaaaa     2940

gcatgtttat aaatcttttg ttgtccttta ccaggtatgt actggggcaa agagcgaaca     3000

acaatcaaag cttgcagcaa gaaaggtatg gggagtattt attttatttc attttatttt     3060

gctacgtgaa gtctaacact tttttattgc cagtatgctc gtattatcca aaagcttggc     3120

tttgctgcta agtttaaggt aactatttca ggcttgatta attttcatct gtgcaaatgc     3180

ctaccatttt cattctgtaa gattgtacct aaatgtttaa ctcatttgca ctataccagg     3240

acttcaagat tcagaacatt gttggttctt gtgatgttaa atttccaatc aggctggagg     3300

gacttgcata ttctcatggt gctttctcaa gtgtaagttg aatccttgca tgttttttta     3360

ggatatttct ttcacatatg ttatgctatt ctcatgtctt gtgccttttg tcttccagta     3420

tgagcctgaa ctctttcctg gtctgatata tcggatgaag caaccgaaga ttgttcttct     3480

gatttttgtt tcaggcaaga ttgttttgac cggagcaaag gtaagcagcc tttccttttg     3540

tatacccttg attgtctatt cctttttgta tgtctgaatg tacttgtctt tataggtgag     3600

ggatgagacg tataccgcct ttgagaacat ataccctgtg ctaacagagt tcagaaaagt     3660

ccagcaatgg tacgtcttta ttttgttgtt taggtatgca gtgtgtggta gaaacatcgg     3720

aagttcgaag taagaattat acagtggata attgcacttg tgtcttttgt tatacgtggg     3780

agtgaacttt gagcacactc cttactaaat tgcaatatgt tgaatttttt aatattacaa     3840

gcacctataa atctgaactt tccaatttga atccagatgg tcagcagaaa ctgaaggaat     3900

aaatcatctc aagaaagatt ttaatttcca cactaccttg atcttagata ggatttggat     3960

acttgtttca gatatgattc atcactacca tgtgatggac tgttggctgc ccttgcctac     4020

ttactgtgct tgactgcagt ttacttccaa tttaggagaa cacaatttta aataataata     4080

ataatgatgg ctccttgcac cttcaaattt gcagataacc tatggaggtc acaactacaa     4140

cgcttccttg aggattttgc tgccttgtaa ctgctaattt taatctgtac atatatgtag     4200

tctggagggg cgtacagcat cttgtaattt atgtgagccc ctggatgaat gagcactgta     4260

gacttgtagc tgggtgagta tgttgttagt agtctcttgt ggcatggagt tcagtccaac     4320

cgatctgatg gagttttcgt acgtttgtag cccttgccga tctttttccc ttttcttccc     4380

aatagacatg ttgctaaact tttactaact tgttaacaga cagacagaat gataacatgg     4440

actgtggatg cttagcgttt gtggccg                                         4467


<210>  34
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-sense protospacer sequence

<400>  34
aaactgcgcg tcagctctta tagc                                              24


<210>  35
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-sense protospacer sequence

<400>  35
aaacgagccg cgctttccgc tatg                                              24


<210>  36
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-sense protospacer sequence

<400>  36
aaacgttgcc ctctcgccgc accc                                              24


<210>  37
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-sense protospace sequence

<400>  37
aaaccgacgc gagcgcggag acgg                                              24


