SEQUENCE LISTING

<110>   KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN 

<120>   IMPROVED CULTURE MEDOD USING INTEGRIN AGONIST

<130>   P075582WO

<140>
<141>   2020-05-18

<160>   10

<170>   SeqWin2010, version 1.0

<210>   1
<211>   470
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   TS2/16 H-chain

<400>   1
Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      

Val His Ser Met Asp Val Lys Leu Val Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          

Lys Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr 
        35                  40                  45              

Phe Ser Ser Tyr Thr Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg 
    50                  55                  60                  

Leu Glu Trp Val Ala Thr Ile Ser Ser Gly Gly Ser Tyr Thr Tyr Tyr 
65                  70                  75                  80  

Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Lys Ala Lys 
                85                  90                  95      

Asn Thr Leu Tyr Leu Gln Met Gly Ser Leu Lys Ser Glu Asp Thr Ala 
            100                 105                 110         

Met Tyr Tyr Cys Thr Arg Ile Gly Tyr Asp Glu Asp Tyr Ala Met Asp 
        115                 120                 125             

His Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala Ser Thr Lys 
    130                 135                 140                 

Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
145                 150                 155                 160 

Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
                165                 170                 175     

Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
            180                 185                 190         

Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
        195                 200                 205             

Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
    210                 215                 220                 

Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
225                 230                 235                 240 

Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
                245                 250                 255     

Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
            260                 265                 270         

Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
        275                 280                 285             

Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
    290                 295                 300                 

Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
305                 310                 315                 320 

Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
                325                 330                 335     

Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
            340                 345                 350         

Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
        355                 360                 365             

Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn 
    370                 375                 380                 

Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
385                 390                 395                 400 

Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
                405                 410                 415     

Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
            420                 425                 430         

Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
        435                 440                 445             

Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
    450                 455                 460                 

Ser Leu Ser Pro Gly Lys 
465                 470 

<210>   2
<211>   234
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   TS2-16  kL-chain

<400>   2
Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      

Val His Ser Glu Ile Val Val Thr Gln Ser Pro Thr Thr Met Ala Ala 
            20                  25                  30          

Ser Pro Gly Asp Lys Ile Thr Ile Thr Cys Ser Val Ser Ser Ile Ile 
        35                  40                  45              

Ser Ser Asn Tyr Leu His Trp Tyr Ser Gln Lys Pro Gly Phe Ser Pro 
    50                  55                  60                  

Lys Leu Leu Ile Tyr Arg Thr Ser Asn Leu Ala Ser Gly Val Pro Pro 
65                  70                  75                  80  

Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Gly 
                85                  90                  95      

Thr Met Glu Ala Glu Asp Val Ala Thr Tyr Tyr Cys Gln Gln Gly Ser 
            100                 105                 110         

Asp Ile Pro Leu Thr Phe Gly Asp Gly Thr Lys Leu Asp Leu Lys Arg 
        115                 120                 125             

Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
    130                 135                 140                 

Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
145                 150                 155                 160 

Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
                165                 170                 175     

Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
            180                 185                 190         

Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
        195                 200                 205             

His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
    210                 215                 220                 

Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 

<210>   3
<211>   121
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Humanised TS2/16 H-chain

<400>   3
Met Asp Val Lys Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly 
1               5                   10                  15      

Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser 
            20                  25                  30          

Tyr Thr Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp 
        35                  40                  45              

Val Ala Thr Ile Ser Ser Gly Gly Ser Tyr Thr Tyr Tyr Pro Asp Ser 
    50                  55                  60                  

Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Lys Ala Lys Asn Thr Leu 
65                  70                  75                  80  

Tyr Leu Gln Met Gly Ser Leu Lys Ser Glu Asp Thr Ala Met Tyr Tyr 
                85                  90                  95      

Cys Thr Arg Ile Gly Tyr Asp Glu Asp Tyr Ala Met Asp His Trp Gly 
            100                 105                 110         

Gln Gly Thr Ser Val Thr Val Ser Ser 
        115                 120     

<210>   4
<211>   108
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Humanised TS2/16 L-chain

<400>   4
Glu Ile Val Val Thr Gln Ser Pro Thr Thr Met Ala Ala Ser Pro Gly 
1               5                   10                  15      

Asp Lys Ile Thr Ile Thr Cys Ser Val Ser Ser Ile Ile Ser Ser Asn 
            20                  25                  30          

Tyr Leu His Trp Tyr Ser Gln Lys Pro Gly Phe Ser Pro Lys Leu Leu 
        35                  40                  45              

Ile Tyr Arg Thr Ser Asn Leu Ala Ser Gly Val Pro Pro Arg Phe Ser 
    50                  55                  60                  

Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Gly Thr Met Glu 
65                  70                  75                  80  

Ala Glu Asp Val Ala Thr Tyr Tyr Cys Gln Gln Gly Ser Asp Ile Pro 
                85                  90                  95      

Leu Thr Phe Gly Asp Gly Thr Lys Leu Asp Leu Lys 
            100                 105             

<210>   5
<211>   10
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Humanised TS2/16 H-chain HCDR1

<400>   5
Gly Phe Thr Phe Ser Ser Tyr Thr Met Ser 
1               5                   10  

<210>   6
<211>   17
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Humanised TS2/16 H-chain HCDR2

<400>   6
Thr Ile Ser Ser Gly Gly Ser Tyr Thr Tyr Tyr Pro Asp Ser Val Lys 
1               5                   10                  15      

Gly 
    

<210>   7
<211>   11
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Humanised TS2/16 H-chain HCDR3

<400>   7
Ile Gly Tyr Asp Glu Asp Tyr Ala Met Asp His 
1               5                   10      

<210>   8
<211>   12
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Humanised TS2/16 L-chain LCDR1

<400>   8
Ser Val Ser Ser Ile Ile Ser Ser Asn Tyr Leu His 
1               5                   10          

<210>   9
<211>   12
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Humanised TS2/16 L-chain LCDR2

<400>   9
Ser Val Ser Ser Ile Ile Ser Ser Asn Tyr Leu His 
1               5                   10          

<210>   10
<211>   9
<212>   PRT
<213>   Humanised TS2/16 L-chain LCDR3

<400>   10
Gln Gln Gly Ser Asp Ile Pro Leu Thr 
1               5                   

