﻿                         序列表

<110>  广州妇女儿童医疗中心

<120>  一种基于内含肽介导纳米载体模块平台及其构建方法与应用

<130>  OWO20005455

<150>  CN201910421408.9
<151>  2019-5-21

<160>  20

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  238
<212>  PRT
<213>  GFP

<400>  1
Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      
Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          
Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              
Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  
Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  
His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      
Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         
Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             
Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 
Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 
Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     
Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         
Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
        195                 200                 205             
Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 
Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             

<210>  2
<211>  8
<212>  PRT
<213>  strep2

<400>  2
Trp Ser His Pro Gln Phe Glu Lys 
1               5               

<210>  3
<211>  42
<212>  PRT
<213>  gp41-1-inteinC

<400>  3
Met Leu Lys Lys Ile Leu Lys Ile Glu Glu Leu Asp Glu Arg Glu Leu 
1               5                   10                  15      
Ile Asp Ile Glu Val Ser Gly Asn His Leu Phe Tyr Ala Asn Asp Ile 
            20                  25                  30          
Leu Thr His Asn Ser Ser Ser Ser Asp Val 
        35                  40          

<210>  4
<211>  93
<212>  PRT
<213>  gp41-1-inteinN

<400>  4
Thr Arg Ser Gly Tyr Cys Leu Asp Leu Lys Thr Gln Val Gln Thr Pro 
1               5                   10                  15      
Gln Gly Met Lys Glu Ile Ser Asn Ile Gln Val Gly Asp Leu Val Leu 
            20                  25                  30          
Ser Asn Thr Gly Tyr Asn Glu Val Leu Asn Val Phe Pro Lys Ser Lys 
        35                  40                  45              
Lys Lys Ser Tyr Lys Ile Thr Leu Glu Asp Gly Lys Glu Ile Ile Cys 
    50                  55                  60                  
Ser Glu Glu His Leu Phe Pro Thr Gln Thr Gly Glu Met Asn Ile Ser 
65                  70                  75                  80  
Gly Gly Leu Lys Glu Gly Met Cys Leu Tyr Val Lys Glu 
                85                  90              

<210>  5
<211>  16
<212>  PRT
<213>  SP70

<400>  5
Tyr Pro Thr Phe Gly Glu His Lys Gln Glu Lys Asp Leu Glu Tyr Gly 
1               5                   10                  15      

<210>  6
<211>  56
<212>  PRT
<213>  gb1

<400>  6
Gln Tyr Lys Leu Ile Leu Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr 
1               5                   10                  15      
Thr Glu Ala Val Asp Ala Ala Thr Ala Glu Lys Val Phe Lys Gln Tyr 
            20                  25                  30          
Ala Asn Asp Asn Gly Val Asp Gly Glu Trp Thr Tyr Asp Asp Ala Thr 
        35                  40                  45              
Lys Thr Phe Thr Val Thr Glu Lys 
    50                  55      

<210>  7
<211>  128
<212>  PRT
<213>  Rhizavidin

<400>  7
Phe Asp Ala Ser Asn Phe Lys Asp Phe Ser Ser Ile Ala Ser Ala Ser 
1               5                   10                  15      
Ser Ser Trp Gln Asn Gln His Gly Ser Thr Met Ile Ile Gln Val Asp 
            20                  25                  30          
Ser Phe Gly Asn Val Ser Gly Gln Tyr Val Asn Arg Ala Glu Gly Thr 
        35                  40                  45              
Gly Cys Gln Asn Ser Pro Tyr Pro Leu Thr Gly Arg Val Asn Gly Thr 
    50                  55                  60                  
Phe Ile Asp Phe Ser Val Lys Trp Asn Asn Ser Thr Glu Asn Cys Asn 
65                  70                  75                  80  
Ser Asn Thr Gln Trp Thr Gly Tyr Ala Gln Val Asn Gly Asn Asn Thr 
                85                  90                  95      
Glu Ile Val Thr Arg Trp Asn Leu Lys Tyr Glu Gly Gly Ser Gly Pro 
            100                 105                 110         
Ala Ile Trp Gln Gly Gln Asp Thr Phe Gln Tyr Val Pro Thr Thr Glu 
        115                 120                 125             

<210>  8
<211>  295
<212>  PRT
<213>  CBLB

<400>  8
Ala Gln Val Ile Asn Thr Asn Ser Leu Ser Leu Leu Thr Gln Asn Asn 
1               5                   10                  15      
Leu Asn Lys Ser Gln Ser Ser Leu Ser Ser Ala Ile Glu Arg Leu Ser 
            20                  25                  30          
Ser Gly Leu Arg Ile Asn Ser Ala Lys Asp Asp Ala Ala Gly Gln Ala 
        35                  40                  45              
Ile Ala Asn Arg Phe Thr Ser Asn Ile Lys Gly Leu Thr Gln Ala Ser 
    50                  55                  60                  
Arg Asn Ala Asn Asp Gly Ile Ser Ile Ala Gln Thr Thr Glu Gly Ala 
65                  70                  75                  80  
Leu Asn Glu Ile Asn Asn Asn Leu Gln Arg Val Arg Glu Leu Ser Val 
                85                  90                  95      
Gln Ala Thr Asn Gly Thr Asn Ser Asp Ser Asp Leu Lys Ser Ile Gln 
            100                 105                 110         
Asp Glu Ile Gln Gln Arg Leu Glu Glu Ile Asp Arg Val Ser Asn Gln 
        115                 120                 125             
Thr Gln Phe Asn Gly Val Lys Val Leu Ser Gln Asp Asn Gln Met Lys 
    130                 135                 140                 
Ile Gln Val Gly Ala Asn Asp Gly Glu Thr Ile Thr Ile Asp Leu Gln 
145                 150                 155                 160 
Lys Ile Asp Val Lys Ser Leu Gly Leu Asp Gly Phe Asn Val Asn Ser 
                165                 170                 175     
Pro Gly Ile Ser Gly Gly Gly Gly Gly Ile Leu Asp Ser Met Gly Thr 
            180                 185                 190         
Leu Ile Asn Glu Asp Ala Ala Ala Ala Lys Lys Ser Thr Ala Asn Pro 
        195                 200                 205             
Leu Ala Ser Ile Asp Ser Ala Leu Ser Lys Val Asp Ala Val Arg Ser 
    210                 215                 220                 
Ser Leu Gly Ala Ile Gln Asn Arg Phe Asp Ser Ala Ile Thr Asn Leu 
225                 230                 235                 240 
Gly Asn Thr Val Thr Asn Leu Asn Ser Ala Arg Ser Arg Ile Glu Asp 
                245                 250                 255     
Ala Asp Tyr Ala Thr Glu Val Ser Asn Met Ser Lys Ala Gln Ile Leu 
            260                 265                 270         
Gln Gln Ala Gly Thr Ser Val Leu Ala Gln Ala Asn Gln Val Pro Gln 
        275                 280                 285             
Asn Val Leu Ser Leu Leu Arg 
    290                 295 

<210>  9
<211>  172
<212>  PRT
<213>  H1HA10

<400>  9
Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser 
1               5                   10                  15      
Val Asn Leu Leu Glu Asp Ser His Gly Ser Ala Asn Ser Ser Leu Pro 
            20                  25                  30          
Tyr Gln Asn Thr His Pro Thr Thr Asn Gly Glu Ser Pro Lys Tyr Val 
        35                  40                  45              
Arg Ser Ala Lys Leu Arg Met Val Thr Gly Leu Arg Asn Gly Ser Ala 
    50                  55                  60                  
Gly Ser Ala Thr Gln Asn Ala Ile Asn Gly Ile Thr Asn Lys Val Asn 
65                  70                  75                  80  
Thr Val Ile Glu Lys Met Asn Ile Gln Asp Thr Ala Thr Gly Lys Glu 
                85                  90                  95      
Phe Asn Lys Asp Glu Lys Arg Met Glu Asn Leu Asn Lys Lys Val Asp 
            100                 105                 110         
Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu 
        115                 120                 125             
Leu Glu Asn Glu Arg Thr Leu Asp Ala His Asp Ser Gln Gly Thr Gly 
    130                 135                 140                 
Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg 
145                 150                 155                 160 
Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu 
                165                 170         

<210>  10
<211>  182
<212>  PRT
<213>  HFT

<400>  10
Thr Thr Ala Ser Thr Ser Gln Val Arg Gln Asn Tyr His Gln Asp Ser 
1               5                   10                  15      
Glu Ala Ala Ile Asn Arg Gln Ile Asn Leu Glu Leu Tyr Ala Ser Tyr 
            20                  25                  30          
Val Tyr Leu Ser Met Ser Tyr Tyr Phe Asp Arg Asp Asp Val Ala Leu 
        35                  40                  45              
Lys Asn Phe Ala Lys Tyr Phe Leu His Gln Ser His Glu Glu Arg Glu 
    50                  55                  60                  
His Ala Glu Lys Leu Met Lys Leu Gln Asn Gln Arg Gly Gly Arg Ile 
65                  70                  75                  80  
Phe Leu Gln Asp Ile Lys Lys Pro Asp Cys Asp Asp Trp Glu Ser Gly 
                85                  90                  95      
Leu Asn Ala Met Glu Cys Ala Leu His Leu Glu Lys Asn Val Asn Gln 
            100                 105                 110         
Ser Leu Leu Glu Leu His Lys Leu Ala Thr Asp Lys Asn Asp Pro His 
        115                 120                 125             
Leu Cys Asp Phe Ile Glu Thr His Tyr Leu Asn Glu Gln Val Lys Ala 
    130                 135                 140                 
Ile Lys Glu Leu Gly Asp His Val Thr Asn Leu Arg Lys Met Gly Ala 
145                 150                 155                 160 
Pro Glu Ser Gly Leu Ala Glu Tyr Leu Phe Asp Lys His Thr Leu Gly 
                165                 170                 175     
Asp Ser Asp Asn Glu Ser 
            180         

<210>  11
<211>  173
<212>  PRT
<213>  PFT

<400>  11
Leu Ser Glu Arg Met Leu Lys Ala Leu Asn Asp Gln Leu Asn Arg Glu 
1               5                   10                  15      
Leu Tyr Ser Ala Tyr Leu Tyr Phe Ala Met Ala Ala Tyr Phe Glu Asp 
            20                  25                  30          
Leu Gly Leu Glu Gly Phe Ala Asn Trp Met Lys Ala Gln Ala Glu Glu 
        35                  40                  45              
Glu Ile Gly His Ala Pro Arg Phe Tyr Asn Tyr Ile Tyr Asp Arg Asn 
    50                  55                  60                  
Gly Arg Val Glu Leu Asp Glu Ile Pro Lys Pro Pro Lys Glu Trp Glu 
65                  70                  75                  80  
Ser Pro Leu Lys Ala Phe Glu Ala Ala Tyr Glu His Glu Lys Phe Ile 
                85                  90                  95      
Ser Lys Ser Ile Tyr Glu Leu Ala Ala Leu Ala Glu Glu Glu Lys Asp 
            100                 105                 110         
Tyr Ser Thr Arg Ala Phe Leu Glu Trp Phe Ile Asn Glu Gln Val Glu 
        115                 120                 125             
Glu Glu Ala Ser Val Lys Lys Ile Leu Asp Lys Leu Lys Phe Ala Lys 
    130                 135                 140                 
Asp Ser Pro Gln Ile Leu Phe Met Leu Asp Lys Glu Leu Ser Ala Arg 
145                 150                 155                 160 
Ala Pro Lys Leu Pro Gly Leu Leu Met Gln Gly Gly Glu 
                165                 170             

<210>  12
<211>  405
<212>  PRT
<213>  strep2-GFP-gp41-1-inteinN-gb1-6xHis

<400>  12
Met Gly Trp Ser His Pro Gln Phe Glu Lys Val Ser Lys Gly Glu Glu 
1               5                   10                  15      
Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val 
            20                  25                  30          
Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr 
        35                  40                  45              
Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro 
    50                  55                  60                  
Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys 
65                  70                  75                  80  
Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser 
                85                  90                  95      
Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp 
            100                 105                 110         
Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr 
        115                 120                 125             
Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly 
    130                 135                 140                 
Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val 
145                 150                 155                 160 
Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys 
                165                 170                 175     
Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr 
            180                 185                 190         
Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn 
        195                 200                 205             
His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys 
    210                 215                 220                 
Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr 
225                 230                 235                 240 
Leu Gly Met Asp Glu Leu Tyr Lys Thr Arg Ser Gly Tyr Cys Leu Asp 
                245                 250                 255     
Leu Lys Thr Gln Val Gln Thr Pro Gln Gly Met Lys Glu Ile Ser Asn 
            260                 265                 270         
Ile Gln Val Gly Asp Leu Val Leu Ser Asn Thr Gly Tyr Asn Glu Val 
        275                 280                 285             
Leu Asn Val Phe Pro Lys Ser Lys Lys Lys Ser Tyr Lys Ile Thr Leu 
    290                 295                 300                 
Glu Asp Gly Lys Glu Ile Ile Cys Ser Glu Glu His Leu Phe Pro Thr 
305                 310                 315                 320 
Gln Thr Gly Glu Met Asn Ile Ser Gly Gly Leu Lys Glu Gly Met Cys 
                325                 330                 335     
Leu Tyr Val Lys Glu Gln Tyr Lys Leu Ile Leu Asn Gly Lys Thr Leu 
            340                 345                 350         
Lys Gly Glu Thr Thr Thr Glu Ala Val Asp Ala Ala Thr Ala Glu Lys 
        355                 360                 365             
Val Phe Lys Gln Tyr Ala Asn Asp Asn Gly Val Asp Gly Glu Trp Thr 
    370                 375                 380                 
Tyr Asp Asp Ala Thr Lys Thr Phe Thr Val Thr Glu Lys Leu Glu His 
385                 390                 395                 400 
His His His His His 
                405 

<210>  13
<211>  172
<212>  PRT
<213>  strep2-gp41-1-inteinN-gb1-6xHis

<400>  13
Met Gly Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Gly Ser Thr 
1               5                   10                  15      
Arg Ser Gly Tyr Cys Leu Asp Leu Lys Thr Gln Val Gln Thr Pro Gln 
            20                  25                  30          
Gly Met Lys Glu Ile Ser Asn Ile Gln Val Gly Asp Leu Val Leu Ser 
        35                  40                  45              
Asn Thr Gly Tyr Asn Glu Val Leu Asn Val Phe Pro Lys Ser Lys Lys 
    50                  55                  60                  
Lys Ser Tyr Lys Ile Thr Leu Glu Asp Gly Lys Glu Ile Ile Cys Ser 
65                  70                  75                  80  
Glu Glu His Leu Phe Pro Thr Gln Thr Gly Glu Met Asn Ile Ser Gly 
                85                  90                  95      
Gly Leu Lys Glu Gly Met Cys Leu Tyr Val Lys Glu Gln Tyr Lys Leu 
            100                 105                 110         
Ile Leu Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr Thr Glu Ala Val 
        115                 120                 125             
Asp Ala Ala Thr Ala Glu Lys Val Phe Lys Gln Tyr Ala Asn Asp Asn 
    130                 135                 140                 
Gly Val Asp Gly Glu Trp Thr Tyr Asp Asp Ala Thr Lys Thr Phe Thr 
145                 150                 155                 160 
Val Thr Glu Lys Leu Glu His His His His His His 
                165                 170         

<210>  14
<211>  185
<212>  PRT
<213>  SP70-gp41-1-inteinN-gb1-strep2-6xHis

<400>  14
Met Gly Tyr Pro Thr Phe Gly Glu His Lys Gln Glu Lys Asp Leu Glu 
1               5                   10                  15      
Tyr Gly Thr Arg Ser Gly Tyr Cys Leu Asp Leu Lys Thr Gln Val Gln 
            20                  25                  30          
Thr Pro Gln Gly Met Lys Glu Ile Ser Asn Ile Gln Val Gly Asp Leu 
        35                  40                  45              
Val Leu Ser Asn Thr Gly Tyr Asn Glu Val Leu Asn Val Phe Pro Lys 
    50                  55                  60                  
Ser Lys Lys Lys Ser Tyr Lys Ile Thr Leu Glu Asp Gly Lys Glu Ile 
65                  70                  75                  80  
Ile Cys Ser Glu Glu His Leu Phe Pro Thr Gln Thr Gly Glu Met Asn 
                85                  90                  95      
Ile Ser Gly Gly Leu Lys Glu Gly Met Cys Leu Tyr Val Lys Glu Gln 
            100                 105                 110         
Tyr Lys Leu Ile Leu Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr Thr 
        115                 120                 125             
Glu Ala Val Asp Ala Ala Thr Ala Glu Lys Val Phe Lys Gln Tyr Ala 
    130                 135                 140                 
Asn Asp Asn Gly Val Asp Gly Glu Trp Thr Tyr Asp Asp Ala Thr Lys 
145                 150                 155                 160 
Thr Phe Thr Val Thr Glu Lys Trp Ser His Pro Gln Phe Glu Lys Gly 
                165                 170                 175     
Ser Leu Glu His His His His His His 
            180                 185 

<210>  15
<211>  317
<212>  PRT
<213>  rhizavidin-gp41-1-intN-gb1-6xHis

<400>  15
Met Gly Phe Asp Ala Ser Asn Phe Lys Asp Phe Ser Ser Ile Ala Ser 
1               5                   10                  15      
Ala Ser Ser Ser Trp Gln Asn Gln His Gly Ser Thr Met Ile Ile Gln 
            20                  25                  30          
Val Asp Ser Phe Gly Asn Val Ser Gly Gln Tyr Val Asn Arg Ala Glu 
        35                  40                  45              
Gly Thr Gly Cys Gln Asn Ser Pro Tyr Pro Leu Thr Gly Arg Val Asn 
    50                  55                  60                  
Gly Thr Phe Ile Asp Phe Ser Val Lys Trp Asn Asn Ser Thr Glu Asn 
65                  70                  75                  80  
Cys Asn Ser Asn Thr Gln Trp Thr Gly Tyr Ala Gln Val Asn Gly Asn 
                85                  90                  95      
Asn Thr Glu Ile Val Thr Arg Trp Asn Leu Lys Tyr Glu Gly Gly Ser 
            100                 105                 110         
Gly Pro Ala Ile Trp Gln Gly Gln Asp Thr Phe Gln Tyr Val Pro Thr 
        115                 120                 125             
Thr Glu Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala 
    130                 135                 140                 
Ala Lys Ala Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Thr Arg 
145                 150                 155                 160 
Ser Gly Tyr Cys Leu Asp Leu Lys Thr Gln Val Gln Thr Pro Gln Gly 
                165                 170                 175     
Met Lys Glu Ile Ser Asn Ile Gln Val Gly Asp Leu Val Leu Ser Asn 
            180                 185                 190         
Thr Gly Tyr Asn Glu Val Leu Asn Val Phe Pro Lys Ser Lys Lys Lys 
        195                 200                 205             
Ser Tyr Lys Ile Thr Leu Glu Asp Gly Lys Glu Ile Ile Cys Ser Glu 
    210                 215                 220                 
Glu His Leu Phe Pro Thr Gln Thr Gly Glu Met Asn Ile Ser Gly Gly 
225                 230                 235                 240 
Leu Lys Glu Gly Met Cys Leu Tyr Val Lys Glu Gln Tyr Lys Leu Ile 
                245                 250                 255     
Leu Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr Thr Glu Ala Val Asp 
            260                 265                 270         
Ala Ala Thr Ala Glu Lys Val Phe Lys Gln Tyr Ala Asn Asp Asn Gly 
        275                 280                 285             
Val Asp Gly Glu Trp Thr Tyr Asp Asp Ala Thr Lys Thr Phe Thr Val 
    290                 295                 300                 
Thr Glu Lys Gly Ser Leu Glu His His His His His His 
305                 310                 315         

<210>  16
<211>  408
<212>  PRT
<213>  CBLB-gp41-1-inteinN-strep2-6xHis

<400>  16
Met Gly Ala Gln Val Ile Asn Thr Asn Ser Leu Ser Leu Leu Thr Gln 
1               5                   10                  15      
Asn Asn Leu Asn Lys Ser Gln Ser Ser Leu Ser Ser Ala Ile Glu Arg 
            20                  25                  30          
Leu Ser Ser Gly Leu Arg Ile Asn Ser Ala Lys Asp Asp Ala Ala Gly 
        35                  40                  45              
Gln Ala Ile Ala Asn Arg Phe Thr Ser Asn Ile Lys Gly Leu Thr Gln 
    50                  55                  60                  
Ala Ser Arg Asn Ala Asn Asp Gly Ile Ser Ile Ala Gln Thr Thr Glu 
65                  70                  75                  80  
Gly Ala Leu Asn Glu Ile Asn Asn Asn Leu Gln Arg Val Arg Glu Leu 
                85                  90                  95      
Ser Val Gln Ala Thr Asn Gly Thr Asn Ser Asp Ser Asp Leu Lys Ser 
            100                 105                 110         
Ile Gln Asp Glu Ile Gln Gln Arg Leu Glu Glu Ile Asp Arg Val Ser 
        115                 120                 125             
Asn Gln Thr Gln Phe Asn Gly Val Lys Val Leu Ser Gln Asp Asn Gln 
    130                 135                 140                 
Met Lys Ile Gln Val Gly Ala Asn Asp Gly Glu Thr Ile Thr Ile Asp 
145                 150                 155                 160 
Leu Gln Lys Ile Asp Val Lys Ser Leu Gly Leu Asp Gly Phe Asn Val 
                165                 170                 175     
Asn Ser Pro Gly Ile Ser Gly Gly Gly Gly Gly Ile Leu Asp Ser Met 
            180                 185                 190         
Gly Thr Leu Ile Asn Glu Asp Ala Ala Ala Ala Lys Lys Ser Thr Ala 
        195                 200                 205             
Asn Pro Leu Ala Ser Ile Asp Ser Ala Leu Ser Lys Val Asp Ala Val 
    210                 215                 220                 
Arg Ser Ser Leu Gly Ala Ile Gln Asn Arg Phe Asp Ser Ala Ile Thr 
225                 230                 235                 240 
Asn Leu Gly Asn Thr Val Thr Asn Leu Asn Ser Ala Arg Ser Arg Ile 
                245                 250                 255     
Glu Asp Ala Asp Tyr Ala Thr Glu Val Ser Asn Met Ser Lys Ala Gln 
            260                 265                 270         
Ile Leu Gln Gln Ala Gly Thr Ser Val Leu Ala Gln Ala Asn Gln Val 
        275                 280                 285             
Pro Gln Asn Val Leu Ser Leu Leu Arg Thr Arg Ser Gly Tyr Cys Leu 
    290                 295                 300                 
Asp Leu Lys Thr Gln Val Gln Thr Pro Gln Gly Met Lys Glu Ile Ser 
305                 310                 315                 320 
Asn Ile Gln Val Gly Asp Leu Val Leu Ser Asn Thr Gly Tyr Asn Glu 
                325                 330                 335     
Val Leu Asn Val Phe Pro Lys Ser Lys Lys Lys Ser Tyr Lys Ile Thr 
            340                 345                 350         
Leu Glu Asp Gly Lys Glu Ile Ile Cys Ser Glu Glu His Leu Phe Pro 
        355                 360                 365             
Thr Gln Thr Gly Glu Met Asn Ile Ser Gly Gly Leu Lys Glu Gly Met 
    370                 375                 380                 
Cys Leu Tyr Val Lys Glu Trp Ser His Pro Gln Phe Glu Lys Gly Ser 
385                 390                 395                 400 
Leu Glu His His His His His His 
                405             

<210>  17
<211>  424
<212>  PRT
<213>  SP70-CBLB-gp41-1-inteinN-strep2-6xHis

<400>  17
Met Gly Tyr Pro Thr Phe Gly Glu His Lys Gln Glu Lys Asp Leu Glu 
1               5                   10                  15      
Tyr Gly Ala Gln Val Ile Asn Thr Asn Ser Leu Ser Leu Leu Thr Gln 
            20                  25                  30          
Asn Asn Leu Asn Lys Ser Gln Ser Ser Leu Ser Ser Ala Ile Glu Arg 
        35                  40                  45              
Leu Ser Ser Gly Leu Arg Ile Asn Ser Ala Lys Asp Asp Ala Ala Gly 
    50                  55                  60                  
Gln Ala Ile Ala Asn Arg Phe Thr Ser Asn Ile Lys Gly Leu Thr Gln 
65                  70                  75                  80  
Ala Ser Arg Asn Ala Asn Asp Gly Ile Ser Ile Ala Gln Thr Thr Glu 
                85                  90                  95      
Gly Ala Leu Asn Glu Ile Asn Asn Asn Leu Gln Arg Val Arg Glu Leu 
            100                 105                 110         
Ser Val Gln Ala Thr Asn Gly Thr Asn Ser Asp Ser Asp Leu Lys Ser 
        115                 120                 125             
Ile Gln Asp Glu Ile Gln Gln Arg Leu Glu Glu Ile Asp Arg Val Ser 
    130                 135                 140                 
Asn Gln Thr Gln Phe Asn Gly Val Lys Val Leu Ser Gln Asp Asn Gln 
145                 150                 155                 160 
Met Lys Ile Gln Val Gly Ala Asn Asp Gly Glu Thr Ile Thr Ile Asp 
                165                 170                 175     
Leu Gln Lys Ile Asp Val Lys Ser Leu Gly Leu Asp Gly Phe Asn Val 
            180                 185                 190         
Asn Ser Pro Gly Ile Ser Gly Gly Gly Gly Gly Ile Leu Asp Ser Met 
        195                 200                 205             
Gly Thr Leu Ile Asn Glu Asp Ala Ala Ala Ala Lys Lys Ser Thr Ala 
    210                 215                 220                 
Asn Pro Leu Ala Ser Ile Asp Ser Ala Leu Ser Lys Val Asp Ala Val 
225                 230                 235                 240 
Arg Ser Ser Leu Gly Ala Ile Gln Asn Arg Phe Asp Ser Ala Ile Thr 
                245                 250                 255     
Asn Leu Gly Asn Thr Val Thr Asn Leu Asn Ser Ala Arg Ser Arg Ile 
            260                 265                 270         
Glu Asp Ala Asp Tyr Ala Thr Glu Val Ser Asn Met Ser Lys Ala Gln 
        275                 280                 285             
Ile Leu Gln Gln Ala Gly Thr Ser Val Leu Ala Gln Ala Asn Gln Val 
    290                 295                 300                 
Pro Gln Asn Val Leu Ser Leu Leu Arg Thr Arg Ser Gly Tyr Cys Leu 
305                 310                 315                 320 
Asp Leu Lys Thr Gln Val Gln Thr Pro Gln Gly Met Lys Glu Ile Ser 
                325                 330                 335     
Asn Ile Gln Val Gly Asp Leu Val Leu Ser Asn Thr Gly Tyr Asn Glu 
            340                 345                 350         
Val Leu Asn Val Phe Pro Lys Ser Lys Lys Lys Ser Tyr Lys Ile Thr 
        355                 360                 365             
Leu Glu Asp Gly Lys Glu Ile Ile Cys Ser Glu Glu His Leu Phe Pro 
    370                 375                 380                 
Thr Gln Thr Gly Glu Met Asn Ile Ser Gly Gly Leu Lys Glu Gly Met 
385                 390                 395                 400 
Cys Leu Tyr Val Lys Glu Trp Ser His Pro Gln Phe Glu Lys Gly Ser 
                405                 410                 415     
Leu Glu His His His His His His 
            420                 

<210>  18
<211>  340
<212>  PRT
<213>  H1HA10-gp41-1-inteinN-gb1-6xHis

<400>  18
Met Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His 
1               5                   10                  15      
Ser Val Asn Leu Leu Glu Asp Ser His Gly Ser Ala Asn Ser Ser Leu 
            20                  25                  30          
Pro Tyr Gln Asn Thr His Pro Thr Thr Asn Gly Glu Ser Pro Lys Tyr 
        35                  40                  45              
Val Arg Ser Ala Lys Leu Arg Met Val Thr Gly Leu Arg Asn Gly Ser 
    50                  55                  60                  
Ala Gly Ser Ala Thr Gln Asn Ala Ile Asn Gly Ile Thr Asn Lys Val 
65                  70                  75                  80  
Asn Thr Val Ile Glu Lys Met Asn Ile Gln Asp Thr Ala Thr Gly Lys 
                85                  90                  95      
Glu Phe Asn Lys Asp Glu Lys Arg Met Glu Asn Leu Asn Lys Lys Val 
            100                 105                 110         
Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val 
        115                 120                 125             
Leu Leu Glu Asn Glu Arg Thr Leu Asp Ala His Asp Ser Gln Gly Thr 
    130                 135                 140                 
Gly Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val 
145                 150                 155                 160 
Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu Gly Gly Gly 
                165                 170                 175     
Ser Gly Gly Gly Ser Thr Arg Ser Gly Tyr Cys Leu Asp Leu Lys Thr 
            180                 185                 190         
Gln Val Gln Thr Pro Gln Gly Met Lys Glu Ile Ser Asn Ile Gln Val 
        195                 200                 205             
Gly Asp Leu Val Leu Ser Asn Thr Gly Tyr Asn Glu Val Leu Asn Val 
    210                 215                 220                 
Phe Pro Lys Ser Lys Lys Lys Ser Tyr Lys Ile Thr Leu Glu Asp Gly 
225                 230                 235                 240 
Lys Glu Ile Ile Cys Ser Glu Glu His Leu Phe Pro Thr Gln Thr Gly 
                245                 250                 255     
Glu Met Asn Ile Ser Gly Gly Leu Lys Glu Gly Met Cys Leu Tyr Val 
            260                 265                 270         
Lys Glu Gln Tyr Lys Leu Ile Leu Asn Gly Lys Thr Leu Lys Gly Glu 
        275                 280                 285             
Thr Thr Thr Glu Ala Val Asp Ala Ala Thr Ala Glu Lys Val Phe Lys 
    290                 295                 300                 
Gln Tyr Ala Asn Asp Asn Gly Val Asp Gly Glu Trp Thr Tyr Asp Asp 
305                 310                 315                 320 
Ala Thr Lys Thr Phe Thr Val Thr Glu Lys Gly Ser Leu Glu His His 
                325                 330                 335     
His His His His 
            340 

<210>  19
<211>  316
<212>  PRT
<213>  gb1-gp41-1-inteinC-HFT

<400>  19
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly Arg 
            20                  25                  30          
Gly Ser Glu Phe Gln Tyr Lys Leu Ile Leu Asn Gly Lys Thr Leu Lys 
        35                  40                  45              
Gly Glu Thr Thr Thr Glu Ala Val Asp Ala Ala Thr Ala Glu Lys Val 
    50                  55                  60                  
Phe Lys Gln Tyr Ala Asn Asp Asn Gly Val Asp Gly Glu Trp Thr Tyr 
65                  70                  75                  80  
Asp Asp Ala Thr Lys Thr Phe Thr Val Thr Glu Lys Met Leu Lys Lys 
                85                  90                  95      
Ile Leu Lys Ile Glu Glu Leu Asp Glu Arg Glu Leu Ile Asp Ile Glu 
            100                 105                 110         
Val Ser Gly Asn His Leu Phe Tyr Ala Asn Asp Ile Leu Thr His Asn 
        115                 120                 125             
Ser Ser Ser Ser Asp Val Thr Thr Ala Ser Thr Ser Gln Val Arg Gln 
    130                 135                 140                 
Asn Tyr His Gln Asp Ser Glu Ala Ala Ile Asn Arg Gln Ile Asn Leu 
145                 150                 155                 160 
Glu Leu Tyr Ala Ser Tyr Val Tyr Leu Ser Met Ser Tyr Tyr Phe Asp 
                165                 170                 175     
Arg Asp Asp Val Ala Leu Lys Asn Phe Ala Lys Tyr Phe Leu His Gln 
            180                 185                 190         
Ser His Glu Glu Arg Glu His Ala Glu Lys Leu Met Lys Leu Gln Asn 
        195                 200                 205             
Gln Arg Gly Gly Arg Ile Phe Leu Gln Asp Ile Lys Lys Pro Asp Cys 
    210                 215                 220                 
Asp Asp Trp Glu Ser Gly Leu Asn Ala Met Glu Cys Ala Leu His Leu 
225                 230                 235                 240 
Glu Lys Asn Val Asn Gln Ser Leu Leu Glu Leu His Lys Leu Ala Thr 
                245                 250                 255     
Asp Lys Asn Asp Pro His Leu Cys Asp Phe Ile Glu Thr His Tyr Leu 
            260                 265                 270         
Asn Glu Gln Val Lys Ala Ile Lys Glu Leu Gly Asp His Val Thr Asn 
        275                 280                 285             
Leu Arg Lys Met Gly Ala Pro Glu Ser Gly Leu Ala Glu Tyr Leu Phe 
    290                 295                 300                 
Asp Lys His Thr Leu Gly Asp Ser Asp Asn Glu Ser 
305                 310                 315     

<210>  20
<211>  307
<212>  PRT
<213>  gb1-gp41-1-inteinC-PFT

<400>  20
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly Arg 
            20                  25                  30          
Gly Ser Glu Phe Gln Tyr Lys Leu Ile Leu Asn Gly Lys Thr Leu Lys 
        35                  40                  45              
Gly Glu Thr Thr Thr Glu Ala Val Asp Ala Ala Thr Ala Glu Lys Val 
    50                  55                  60                  
Phe Lys Gln Tyr Ala Asn Asp Asn Gly Val Asp Gly Glu Trp Thr Tyr 
65                  70                  75                  80  
Asp Asp Ala Thr Lys Thr Phe Thr Val Thr Glu Lys Met Leu Lys Lys 
                85                  90                  95      
Ile Leu Lys Ile Glu Glu Leu Asp Glu Arg Glu Leu Ile Asp Ile Glu 
            100                 105                 110         
Val Ser Gly Asn His Leu Phe Tyr Ala Asn Asp Ile Leu Thr His Asn 
        115                 120                 125             
Ser Ser Ser Ser Asp Val Leu Ser Glu Arg Met Leu Lys Ala Leu Asn 
    130                 135                 140                 
Asp Gln Leu Asn Arg Glu Leu Tyr Ser Ala Tyr Leu Tyr Phe Ala Met 
145                 150                 155                 160 
Ala Ala Tyr Phe Glu Asp Leu Gly Leu Glu Gly Phe Ala Asn Trp Met 
                165                 170                 175     
Lys Ala Gln Ala Glu Glu Glu Ile Gly His Ala Pro Arg Phe Tyr Asn 
            180                 185                 190         
Tyr Ile Tyr Asp Arg Asn Gly Arg Val Glu Leu Asp Glu Ile Pro Lys 
        195                 200                 205             
Pro Pro Lys Glu Trp Glu Ser Pro Leu Lys Ala Phe Glu Ala Ala Tyr 
    210                 215                 220                 
Glu His Glu Lys Phe Ile Ser Lys Ser Ile Tyr Glu Leu Ala Ala Leu 
225                 230                 235                 240 
Ala Glu Glu Glu Lys Asp Tyr Ser Thr Arg Ala Phe Leu Glu Trp Phe 
                245                 250                 255     
Ile Asn Glu Gln Val Glu Glu Glu Ala Ser Val Lys Lys Ile Leu Asp 
            260                 265                 270         
Lys Leu Lys Phe Ala Lys Asp Ser Pro Gln Ile Leu Phe Met Leu Asp 
        275                 280                 285             
Lys Glu Leu Ser Ala Arg Ala Pro Lys Leu Pro Gly Leu Leu Met Gln 
    290                 295                 300                 
Gly Gly Glu 
305         

