                         SEQUENCE LISTING

<110>  ϺĿҽҩƼ޹˾
 
<120>  һL-Ȱøͻ弰Ӧ

<130>  P20401332C

<150>  CN201910434350.1
<151>  2019-05-23

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  457
<212>  PRT
<213>  Lysinibacillus sphaericus

<400>  1

Met Thr Ile Thr Thr Val Ser Asn Glu Gln Leu Ala Lys Glu Tyr Val 
1               5                   10                  15      


Asp Gly Val Phe Glu Gln Leu Lys Gln Gln Asn Cys His Gln Ala Glu 
            20                  25                  30          


Phe Leu Gln Ala Ala Glu Glu Ile Phe Ile Ser Leu Val Pro Val Phe 
        35                  40                  45              


Val Gln His Pro Glu Tyr Ile Lys Ala Asn Ile Leu Ser Arg Ile Val 
    50                  55                  60                  


Glu Pro Asp Arg Ile Ile Ser Phe Arg Val Ala Trp Gln Asp Asp His 
65                  70                  75                  80  


Asn Gln Val Gln Val Asn Arg Gly Tyr Arg Val Gln Tyr Ser Asn Val 
                85                  90                  95      


Met Gly Pro Tyr Lys Gly Gly Leu Arg Phe His Pro Ser Val Asn Glu 
            100                 105                 110         


Ser Ile Ile Lys Phe Leu Gly Phe Glu Gln Ile Phe Lys Asn Ala Leu 
        115                 120                 125             


Thr Gly Gln Pro Ile Gly Gly Gly Lys Gly Gly Ser Asn Phe Asp Pro 
    130                 135                 140                 


Lys Gly Lys Ser Asp Ser Glu Ile Met Arg Phe Cys Gln Ala Phe Met 
145                 150                 155                 160 


Thr Glu Leu Tyr Arg His Ile Gly Pro Asp Val Asp Val Pro Ala Gly 
                165                 170                 175     


Asp Ile Gly Val Gly Ala Arg Glu Val Gly Tyr Leu Trp Gly Gln Tyr 
            180                 185                 190         


Lys Arg Leu Thr Lys Ala Ser Glu Ser Gly Val Leu Thr Gly Lys Thr 
        195                 200                 205             


Pro Gly Tyr Gly Gly Ser Leu Ala Arg Lys Glu Ala Thr Gly Tyr Gly 
    210                 215                 220                 


Thr Val Tyr Phe Val Asn Glu Met Leu Lys Asp Val Asn Asp Ser Phe 
225                 230                 235                 240 


Glu Gly Lys Thr Val Val Val Ser Gly Ser Gly Asn Val Ser Thr Tyr 
                245                 250                 255     


Ala Ile Glu Lys Ala Gln Gln Tyr Gly Ala Lys Val Val Ala Cys Ser 
            260                 265                 270         


Asp Ser Ser Gly Tyr Ile Tyr Asp Pro Glu Gly Leu Asp Leu Asp Val 
        275                 280                 285             


Ile Lys Glu Ile Lys Glu Val Lys Gly Asp Arg Ile Ser Thr Tyr Val 
    290                 295                 300                 


Ser Tyr Arg Pro Asn Ala Thr Phe Thr Asn Gly Cys Thr Gly Ile Trp 
305                 310                 315                 320 


Thr Ile Pro Cys Asp Ile Ala Leu Pro Cys Ala Thr Gln Asn Glu Ile 
                325                 330                 335     


Asn Gly Glu Ser Ala Arg Thr Leu Ile Ser Asn Gly Val Lys Ala Ile 
            340                 345                 350         


Gly Glu Gly Ala Asn Met Pro Ser Asp Leu Glu Ala Ile Asn Glu Phe 
        355                 360                 365             


Leu Asn Ala Gly Val Leu Phe Gly Pro Ala Lys Ala Ala Asn Ala Gly 
    370                 375                 380                 


Gly Val Ala Val Ser Ala Leu Glu Met Ala Gln Asp Ser Ser Arg Val 
385                 390                 395                 400 


Phe Trp Ser Phe Glu Glu Val Asp Ala Lys Leu His Gln Ile Met Lys 
                405                 410                 415     


Asp Ile Tyr Ser Asp Ser Lys Ala Ala Ala Glu Lys Tyr Gly Phe Pro 
            420                 425                 430         


Gly Asn Leu Val Met Gly Ala Asn Ile Ala Gly Phe Ile Lys Val Ala 
        435                 440                 445             


Asp Gly Met Leu Thr Glu Gly Ile Tyr 
    450                 455         


<210>  2
<211>  1371
<212>  DNA
<213>  Lysinibacillus sphaericus

<400>  2
atgaccatca ccaccgtttc taacgaacag ctggctaaag aatacgttga cggtgttttc       60

gaacagctga aacagcagaa ctgccaccag gctgaattcc tgcaggctgc tgaagaaatc      120

ttcatctctc tggttccggt tttcgttcag cacccggaat acatcaaagc taacatcctg      180

tctcgtatcg ttgaaccgga ccgtatcatc tctttccgtg ttgcttggca ggacgaccac      240

aaccaggttc aggttaaccg tggttaccgt gttcagtact ctaacgttat gggtccgtac      300

aaaggtggtc tgcgtttcca cccgtctgtt aacgaatcta tcatcaaatt cctgggtttc      360

gaacagatct tcaaaaacgc tctgaccggt cagccgatcg gtggtggtaa aggtggttct      420

aacttcgacc cgaaaggtaa atctgactct gaaatcatgc gtttctgcca ggctttcatg      480

accgaactgt accgtcacat cggtccggac gttgacgttc cggctggtga catcggtgtt      540

ggtgctcgtg aagttggtta cctgtggggt cagtacaaac gtctgaccaa agcttctgaa      600

tctggtgttc tgaccggtaa aaccccgggt tacggtggtt ctctggctcg taaagaagct      660

accggttacg gtaccgttta cttcgttaac gaaatgctga aagacgttaa cgactctttc      720

gaaggtaaaa ccgttgttgt ttctggttct ggtaacgttt ctacctacgc tatcgaaaaa      780

gctcagcagt acggtgctaa agttgttgct tgctctgact cttctggtta catctacgac      840

ccggaaggtc tggacctgga cgttatcaaa gaaatcaaag aagttaaagg tgaccgtatc      900

tctacctacg tttcttaccg tccgaacgct accttcacca acggttgcac cggtatctgg      960

accatcccgt gcgacatcgc tctgccgtgc gctacccaga acgaaatcaa cggtgaatct     1020

gctcgtaccc tgatctctaa cggtgttaaa gctatcggtg aaggtgctaa catgccgtct     1080

gacctggaag ctatcaacga attcctgaac gctggtgttc tgttcggtcc ggctaaagct     1140

gctaacgctg gtggtgttgc tgtttctgct ctggaaatgg ctcaggactc ttctcgtgtt     1200

ttctggtctt tcgaagaagt tgacgctaaa ctgcaccaga tcatgaaaga catctactct     1260

gactctaaag ctgctgctga aaaatacggt ttcccgggta acctggttat gggtgctaac     1320

atcgctggtt tcatcaaagt tgctgacggt atgctgaccg aaggtatcta c              1371


<210>  3
<211>  457
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LsGluDH-A175G

<400>  3

Met Thr Ile Thr Thr Val Ser Asn Glu Gln Leu Ala Lys Glu Tyr Val 
1               5                   10                  15      


Asp Gly Val Phe Glu Gln Leu Lys Gln Gln Asn Cys His Gln Ala Glu 
            20                  25                  30          


Phe Leu Gln Ala Ala Glu Glu Ile Phe Ile Ser Leu Val Pro Val Phe 
        35                  40                  45              


Val Gln His Pro Glu Tyr Ile Lys Ala Asn Ile Leu Ser Arg Ile Val 
    50                  55                  60                  


Glu Pro Asp Arg Ile Ile Ser Phe Arg Val Ala Trp Gln Asp Asp His 
65                  70                  75                  80  


Asn Gln Val Gln Val Asn Arg Gly Tyr Arg Val Gln Tyr Ser Asn Val 
                85                  90                  95      


Met Gly Pro Tyr Lys Gly Gly Leu Arg Phe His Pro Ser Val Asn Glu 
            100                 105                 110         


Ser Ile Ile Lys Phe Leu Gly Phe Glu Gln Ile Phe Lys Asn Ala Leu 
        115                 120                 125             


Thr Gly Gln Pro Ile Gly Gly Gly Lys Gly Gly Ser Asn Phe Asp Pro 
    130                 135                 140                 


Lys Gly Lys Ser Asp Ser Glu Ile Met Arg Phe Cys Gln Ala Phe Met 
145                 150                 155                 160 


Thr Glu Leu Tyr Arg His Ile Gly Pro Asp Val Asp Val Pro Gly Gly 
                165                 170                 175     


Asp Ile Gly Val Gly Ala Arg Glu Val Gly Tyr Leu Trp Gly Gln Tyr 
            180                 185                 190         


Lys Arg Leu Thr Lys Ala Ser Glu Ser Gly Val Leu Thr Gly Lys Thr 
        195                 200                 205             


Pro Gly Tyr Gly Gly Ser Leu Ala Arg Lys Glu Ala Thr Gly Tyr Gly 
    210                 215                 220                 


Thr Val Tyr Phe Val Asn Glu Met Leu Lys Asp Val Asn Asp Ser Phe 
225                 230                 235                 240 


Glu Gly Lys Thr Val Val Val Ser Gly Ser Gly Asn Val Ser Thr Tyr 
                245                 250                 255     


Ala Ile Glu Lys Ala Gln Gln Tyr Gly Ala Lys Val Val Ala Cys Ser 
            260                 265                 270         


Asp Ser Ser Gly Tyr Ile Tyr Asp Pro Glu Gly Leu Asp Leu Asp Val 
        275                 280                 285             


Ile Lys Glu Ile Lys Glu Val Lys Gly Asp Arg Ile Ser Thr Tyr Val 
    290                 295                 300                 


Ser Tyr Arg Pro Asn Ala Thr Phe Thr Asn Gly Cys Thr Gly Ile Trp 
305                 310                 315                 320 


Thr Ile Pro Cys Asp Ile Ala Leu Pro Cys Ala Thr Gln Asn Glu Ile 
                325                 330                 335     


Asn Gly Glu Ser Ala Arg Thr Leu Ile Ser Asn Gly Val Lys Ala Ile 
            340                 345                 350         


Gly Glu Gly Ala Asn Met Pro Ser Asp Leu Glu Ala Ile Asn Glu Phe 
        355                 360                 365             


Leu Asn Ala Gly Val Leu Phe Gly Pro Ala Lys Ala Ala Asn Ala Gly 
    370                 375                 380                 


Gly Val Ala Val Ser Ala Leu Glu Met Ala Gln Asp Ser Ser Arg Val 
385                 390                 395                 400 


Phe Trp Ser Phe Glu Glu Val Asp Ala Lys Leu His Gln Ile Met Lys 
                405                 410                 415     


Asp Ile Tyr Ser Asp Ser Lys Ala Ala Ala Glu Lys Tyr Gly Phe Pro 
            420                 425                 430         


Gly Asn Leu Val Met Gly Ala Asn Ile Ala Gly Phe Ile Lys Val Ala 
        435                 440                 445             


Asp Gly Met Leu Thr Glu Gly Ile Tyr 
    450                 455         


<210>  4
<211>  1371
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LsGluDH-A175G

<400>  4
atgaccatca ccaccgtttc taacgaacag ctggctaaag aatacgttga cggtgttttc       60

gaacagctga aacagcagaa ctgccaccag gctgaattcc tgcaggctgc tgaagaaatc      120

ttcatctctc tggttccggt tttcgttcag cacccggaat acatcaaagc taacatcctg      180

tctcgtatcg ttgaaccgga ccgtatcatc tctttccgtg ttgcttggca ggacgaccac      240

aaccaggttc aggttaaccg tggttaccgt gttcagtact ctaacgttat gggtccgtac      300

aaaggtggtc tgcgtttcca cccgtctgtt aacgaatcta tcatcaaatt cctgggtttc      360

gaacagatct tcaaaaacgc tctgaccggt cagccgatcg gtggtggtaa aggtggttct      420

aacttcgacc cgaaaggtaa atctgactct gaaatcatgc gtttctgcca ggctttcatg      480

accgaactgt accgtcacat cggtccggac gttgacgttc cgggtggtga catcggtgtt      540

ggtgctcgtg aagttggtta cctgtggggt cagtacaaac gtctgaccaa agcttctgaa      600

tctggtgttc tgaccggtaa aaccccgggt tacggtggtt ctctggctcg taaagaagct      660

accggttacg gtaccgttta cttcgttaac gaaatgctga aagacgttaa cgactctttc      720

gaaggtaaaa ccgttgttgt ttctggttct ggtaacgttt ctacctacgc tatcgaaaaa      780

gctcagcagt acggtgctaa agttgttgct tgctctgact cttctggtta catctacgac      840

ccggaaggtc tggacctgga cgttatcaaa gaaatcaaag aagttaaagg tgaccgtatc      900

tctacctacg tttcttaccg tccgaacgct accttcacca acggttgcac cggtatctgg      960

accatcccgt gcgacatcgc tctgccgtgc gctacccaga acgaaatcaa cggtgaatct     1020

gctcgtaccc tgatctctaa cggtgttaaa gctatcggtg aaggtgctaa catgccgtct     1080

gacctggaag ctatcaacga attcctgaac gctggtgttc tgttcggtcc ggctaaagct     1140

gctaacgctg gtggtgttgc tgtttctgct ctggaaatgg ctcaggactc ttctcgtgtt     1200

ttctggtctt tcgaagaagt tgacgctaaa ctgcaccaga tcatgaaaga catctactct     1260

gactctaaag ctgctgctga aaaatacggt ttcccgggta acctggttat gggtgctaac     1320

atcgctggtt tcatcaaagt tgctgacggt atgctgaccg aaggtatcta c              1371


<210>  5
<211>  457
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LsGluDH-V386A

<400>  5

Met Thr Ile Thr Thr Val Ser Asn Glu Gln Leu Ala Lys Glu Tyr Val 
1               5                   10                  15      


Asp Gly Val Phe Glu Gln Leu Lys Gln Gln Asn Cys His Gln Ala Glu 
            20                  25                  30          


Phe Leu Gln Ala Ala Glu Glu Ile Phe Ile Ser Leu Val Pro Val Phe 
        35                  40                  45              


Val Gln His Pro Glu Tyr Ile Lys Ala Asn Ile Leu Ser Arg Ile Val 
    50                  55                  60                  


Glu Pro Asp Arg Ile Ile Ser Phe Arg Val Ala Trp Gln Asp Asp His 
65                  70                  75                  80  


Asn Gln Val Gln Val Asn Arg Gly Tyr Arg Val Gln Tyr Ser Asn Val 
                85                  90                  95      


Met Gly Pro Tyr Lys Gly Gly Leu Arg Phe His Pro Ser Val Asn Glu 
            100                 105                 110         


Ser Ile Ile Lys Phe Leu Gly Phe Glu Gln Ile Phe Lys Asn Ala Leu 
        115                 120                 125             


Thr Gly Gln Pro Ile Gly Gly Gly Lys Gly Gly Ser Asn Phe Asp Pro 
    130                 135                 140                 


Lys Gly Lys Ser Asp Ser Glu Ile Met Arg Phe Cys Gln Ala Phe Met 
145                 150                 155                 160 


Thr Glu Leu Tyr Arg His Ile Gly Pro Asp Val Asp Val Pro Ala Gly 
                165                 170                 175     


Asp Ile Gly Val Gly Ala Arg Glu Val Gly Tyr Leu Trp Gly Gln Tyr 
            180                 185                 190         


Lys Arg Leu Thr Lys Ala Ser Glu Ser Gly Val Leu Thr Gly Lys Thr 
        195                 200                 205             


Pro Gly Tyr Gly Gly Ser Leu Ala Arg Lys Glu Ala Thr Gly Tyr Gly 
    210                 215                 220                 


Thr Val Tyr Phe Val Asn Glu Met Leu Lys Asp Val Asn Asp Ser Phe 
225                 230                 235                 240 


Glu Gly Lys Thr Val Val Val Ser Gly Ser Gly Asn Val Ser Thr Tyr 
                245                 250                 255     


Ala Ile Glu Lys Ala Gln Gln Tyr Gly Ala Lys Val Val Ala Cys Ser 
            260                 265                 270         


Asp Ser Ser Gly Tyr Ile Tyr Asp Pro Glu Gly Leu Asp Leu Asp Val 
        275                 280                 285             


Ile Lys Glu Ile Lys Glu Val Lys Gly Asp Arg Ile Ser Thr Tyr Val 
    290                 295                 300                 


Ser Tyr Arg Pro Asn Ala Thr Phe Thr Asn Gly Cys Thr Gly Ile Trp 
305                 310                 315                 320 


Thr Ile Pro Cys Asp Ile Ala Leu Pro Cys Ala Thr Gln Asn Glu Ile 
                325                 330                 335     


Asn Gly Glu Ser Ala Arg Thr Leu Ile Ser Asn Gly Val Lys Ala Ile 
            340                 345                 350         


Gly Glu Gly Ala Asn Met Pro Ser Asp Leu Glu Ala Ile Asn Glu Phe 
        355                 360                 365             


Leu Asn Ala Gly Val Leu Phe Gly Pro Ala Lys Ala Ala Asn Ala Gly 
    370                 375                 380                 


Gly Ala Ala Val Ser Ala Leu Glu Met Ala Gln Asp Ser Ser Arg Val 
385                 390                 395                 400 


Phe Trp Ser Phe Glu Glu Val Asp Ala Lys Leu His Gln Ile Met Lys 
                405                 410                 415     


Asp Ile Tyr Ser Asp Ser Lys Ala Ala Ala Glu Lys Tyr Gly Phe Pro 
            420                 425                 430         


Gly Asn Leu Val Met Gly Ala Asn Ile Ala Gly Phe Ile Lys Val Ala 
        435                 440                 445             


Asp Gly Met Leu Thr Glu Gly Ile Tyr 
    450                 455         


<210>  6
<211>  1371
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LsGluDH-V386A

<400>  6
atgaccatca ccaccgtttc taacgaacag ctggctaaag aatacgttga cggtgttttc       60

gaacagctga aacagcagaa ctgccaccag gctgaattcc tgcaggctgc tgaagaaatc      120

ttcatctctc tggttccggt tttcgttcag cacccggaat acatcaaagc taacatcctg      180

tctcgtatcg ttgaaccgga ccgtatcatc tctttccgtg ttgcttggca ggacgaccac      240

aaccaggttc aggttaaccg tggttaccgt gttcagtact ctaacgttat gggtccgtac      300

aaaggtggtc tgcgtttcca cccgtctgtt aacgaatcta tcatcaaatt cctgggtttc      360

gaacagatct tcaaaaacgc tctgaccggt cagccgatcg gtggtggtaa aggtggttct      420

aacttcgacc cgaaaggtaa atctgactct gaaatcatgc gtttctgcca ggctttcatg      480

accgaactgt accgtcacat cggtccggac gttgacgttc cggctggtga catcggtgtt      540

ggtgctcgtg aagttggtta cctgtggggt cagtacaaac gtctgaccaa agcttctgaa      600

tctggtgttc tgaccggtaa aaccccgggt tacggtggtt ctctggctcg taaagaagct      660

accggttacg gtaccgttta cttcgttaac gaaatgctga aagacgttaa cgactctttc      720

gaaggtaaaa ccgttgttgt ttctggttct ggtaacgttt ctacctacgc tatcgaaaaa      780

gctcagcagt acggtgctaa agttgttgct tgctctgact cttctggtta catctacgac      840

ccggaaggtc tggacctgga cgttatcaaa gaaatcaaag aagttaaagg tgaccgtatc      900

tctacctacg tttcttaccg tccgaacgct accttcacca acggttgcac cggtatctgg      960

accatcccgt gcgacatcgc tctgccgtgc gctacccaga acgaaatcaa cggtgaatct     1020

gctcgtaccc tgatctctaa cggtgttaaa gctatcggtg aaggtgctaa catgccgtct     1080

gacctggaag ctatcaacga attcctgaac gctggtgttc tgttcggtcc ggctaaagct     1140

gctaacgctg gtggtgctgc tgtttctgct ctggaaatgg ctcaggactc ttctcgtgtt     1200

ttctggtctt tcgaagaagt tgacgctaaa ctgcaccaga tcatgaaaga catctactct     1260

gactctaaag ctgctgctga aaaatacggt ttcccgggta acctggttat gggtgctaac     1320

atcgctggtt tcatcaaagt tgctgacggt atgctgaccg aaggtatcta c              1371


<210>  7
<211>  457
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LsGluDH-A175G-V386S

<400>  7

Met Thr Ile Thr Thr Val Ser Asn Glu Gln Leu Ala Lys Glu Tyr Val 
1               5                   10                  15      


Asp Gly Val Phe Glu Gln Leu Lys Gln Gln Asn Cys His Gln Ala Glu 
            20                  25                  30          


Phe Leu Gln Ala Ala Glu Glu Ile Phe Ile Ser Leu Val Pro Val Phe 
        35                  40                  45              


Val Gln His Pro Glu Tyr Ile Lys Ala Asn Ile Leu Ser Arg Ile Val 
    50                  55                  60                  


Glu Pro Asp Arg Ile Ile Ser Phe Arg Val Ala Trp Gln Asp Asp His 
65                  70                  75                  80  


Asn Gln Val Gln Val Asn Arg Gly Tyr Arg Val Gln Tyr Ser Asn Val 
                85                  90                  95      


Met Gly Pro Tyr Lys Gly Gly Leu Arg Phe His Pro Ser Val Asn Glu 
            100                 105                 110         


Ser Ile Ile Lys Phe Leu Gly Phe Glu Gln Ile Phe Lys Asn Ala Leu 
        115                 120                 125             


Thr Gly Gln Pro Ile Gly Gly Gly Lys Gly Gly Ser Asn Phe Asp Pro 
    130                 135                 140                 


Lys Gly Lys Ser Asp Ser Glu Ile Met Arg Phe Cys Gln Ala Phe Met 
145                 150                 155                 160 


Thr Glu Leu Tyr Arg His Ile Gly Pro Asp Val Asp Val Pro Gly Gly 
                165                 170                 175     


Asp Ile Gly Val Gly Ala Arg Glu Val Gly Tyr Leu Trp Gly Gln Tyr 
            180                 185                 190         


Lys Arg Leu Thr Lys Ala Ser Glu Ser Gly Val Leu Thr Gly Lys Thr 
        195                 200                 205             


Pro Gly Tyr Gly Gly Ser Leu Ala Arg Lys Glu Ala Thr Gly Tyr Gly 
    210                 215                 220                 


Thr Val Tyr Phe Val Asn Glu Met Leu Lys Asp Val Asn Asp Ser Phe 
225                 230                 235                 240 


Glu Gly Lys Thr Val Val Val Ser Gly Ser Gly Asn Val Ser Thr Tyr 
                245                 250                 255     


Ala Ile Glu Lys Ala Gln Gln Tyr Gly Ala Lys Val Val Ala Cys Ser 
            260                 265                 270         


Asp Ser Ser Gly Tyr Ile Tyr Asp Pro Glu Gly Leu Asp Leu Asp Val 
        275                 280                 285             


Ile Lys Glu Ile Lys Glu Val Lys Gly Asp Arg Ile Ser Thr Tyr Val 
    290                 295                 300                 


Ser Tyr Arg Pro Asn Ala Thr Phe Thr Asn Gly Cys Thr Gly Ile Trp 
305                 310                 315                 320 


Thr Ile Pro Cys Asp Ile Ala Leu Pro Cys Ala Thr Gln Asn Glu Ile 
                325                 330                 335     


Asn Gly Glu Ser Ala Arg Thr Leu Ile Ser Asn Gly Val Lys Ala Ile 
            340                 345                 350         


Gly Glu Gly Ala Asn Met Pro Ser Asp Leu Glu Ala Ile Asn Glu Phe 
        355                 360                 365             


Leu Asn Ala Gly Val Leu Phe Gly Pro Ala Lys Ala Ala Asn Ala Gly 
    370                 375                 380                 


Gly Ser Ala Val Ser Ala Leu Glu Met Ala Gln Asp Ser Ser Arg Val 
385                 390                 395                 400 


Phe Trp Ser Phe Glu Glu Val Asp Ala Lys Leu His Gln Ile Met Lys 
                405                 410                 415     


Asp Ile Tyr Ser Asp Ser Lys Ala Ala Ala Glu Lys Tyr Gly Phe Pro 
            420                 425                 430         


Gly Asn Leu Val Met Gly Ala Asn Ile Ala Gly Phe Ile Lys Val Ala 
        435                 440                 445             


Asp Gly Met Leu Thr Glu Gly Ile Tyr 
    450                 455         


<210>  8
<211>  1371
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LsGluDH-A175G-V386S

<400>  8
atgaccatca ccaccgtttc taacgaacag ctggctaaag aatacgttga cggtgttttc       60

gaacagctga aacagcagaa ctgccaccag gctgaattcc tgcaggctgc tgaagaaatc      120

ttcatctctc tggttccggt tttcgttcag cacccggaat acatcaaagc taacatcctg      180

tctcgtatcg ttgaaccgga ccgtatcatc tctttccgtg ttgcttggca ggacgaccac      240

aaccaggttc aggttaaccg tggttaccgt gttcagtact ctaacgttat gggtccgtac      300

aaaggtggtc tgcgtttcca cccgtctgtt aacgaatcta tcatcaaatt cctgggtttc      360

gaacagatct tcaaaaacgc tctgaccggt cagccgatcg gtggtggtaa aggtggttct      420

aacttcgacc cgaaaggtaa atctgactct gaaatcatgc gtttctgcca ggctttcatg      480

accgaactgt accgtcacat cggtccggac gttgacgttc cgggtggtga catcggtgtt      540

ggtgctcgtg aagttggtta cctgtggggt cagtacaaac gtctgaccaa agcttctgaa      600

tctggtgttc tgaccggtaa aaccccgggt tacggtggtt ctctggctcg taaagaagct      660

accggttacg gtaccgttta cttcgttaac gaaatgctga aagacgttaa cgactctttc      720

gaaggtaaaa ccgttgttgt ttctggttct ggtaacgttt ctacctacgc tatcgaaaaa      780

gctcagcagt acggtgctaa agttgttgct tgctctgact cttctggtta catctacgac      840

ccggaaggtc tggacctgga cgttatcaaa gaaatcaaag aagttaaagg tgaccgtatc      900

tctacctacg tttcttaccg tccgaacgct accttcacca acggttgcac cggtatctgg      960

accatcccgt gcgacatcgc tctgccgtgc gctacccaga acgaaatcaa cggtgaatct     1020

gctcgtaccc tgatctctaa cggtgttaaa gctatcggtg aaggtgctaa catgccgtct     1080

gacctggaag ctatcaacga attcctgaac gctggtgttc tgttcggtcc ggctaaagct     1140

gctaacgctg gtggttctgc tgtttctgct ctggaaatgg ctcaggactc ttctcgtgtt     1200

ttctggtctt tcgaagaagt tgacgctaaa ctgcaccaga tcatgaaaga catctactct     1260

gactctaaag ctgctgctga aaaatacggt ttcccgggta acctggttat gggtgctaac     1320

atcgctggtt tcatcaaagt tgctgacggt atgctgaccg aaggtatcta c              1371


<210>  9
<211>  457
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LsGluDH-A175G-V386A

<400>  9

Met Thr Ile Thr Thr Val Ser Asn Glu Gln Leu Ala Lys Glu Tyr Val 
1               5                   10                  15      


Asp Gly Val Phe Glu Gln Leu Lys Gln Gln Asn Cys His Gln Ala Glu 
            20                  25                  30          


Phe Leu Gln Ala Ala Glu Glu Ile Phe Ile Ser Leu Val Pro Val Phe 
        35                  40                  45              


Val Gln His Pro Glu Tyr Ile Lys Ala Asn Ile Leu Ser Arg Ile Val 
    50                  55                  60                  


Glu Pro Asp Arg Ile Ile Ser Phe Arg Val Ala Trp Gln Asp Asp His 
65                  70                  75                  80  


Asn Gln Val Gln Val Asn Arg Gly Tyr Arg Val Gln Tyr Ser Asn Val 
                85                  90                  95      


Met Gly Pro Tyr Lys Gly Gly Leu Arg Phe His Pro Ser Val Asn Glu 
            100                 105                 110         


Ser Ile Ile Lys Phe Leu Gly Phe Glu Gln Ile Phe Lys Asn Ala Leu 
        115                 120                 125             


Thr Gly Gln Pro Ile Gly Gly Gly Lys Gly Gly Ser Asn Phe Asp Pro 
    130                 135                 140                 


Lys Gly Lys Ser Asp Ser Glu Ile Met Arg Phe Cys Gln Ala Phe Met 
145                 150                 155                 160 


Thr Glu Leu Tyr Arg His Ile Gly Pro Asp Val Asp Val Pro Gly Gly 
                165                 170                 175     


Asp Ile Gly Val Gly Ala Arg Glu Val Gly Tyr Leu Trp Gly Gln Tyr 
            180                 185                 190         


Lys Arg Leu Thr Lys Ala Ser Glu Ser Gly Val Leu Thr Gly Lys Thr 
        195                 200                 205             


Pro Gly Tyr Gly Gly Ser Leu Ala Arg Lys Glu Ala Thr Gly Tyr Gly 
    210                 215                 220                 


Thr Val Tyr Phe Val Asn Glu Met Leu Lys Asp Val Asn Asp Ser Phe 
225                 230                 235                 240 


Glu Gly Lys Thr Val Val Val Ser Gly Ser Gly Asn Val Ser Thr Tyr 
                245                 250                 255     


Ala Ile Glu Lys Ala Gln Gln Tyr Gly Ala Lys Val Val Ala Cys Ser 
            260                 265                 270         


Asp Ser Ser Gly Tyr Ile Tyr Asp Pro Glu Gly Leu Asp Leu Asp Val 
        275                 280                 285             


Ile Lys Glu Ile Lys Glu Val Lys Gly Asp Arg Ile Ser Thr Tyr Val 
    290                 295                 300                 


Ser Tyr Arg Pro Asn Ala Thr Phe Thr Asn Gly Cys Thr Gly Ile Trp 
305                 310                 315                 320 


Thr Ile Pro Cys Asp Ile Ala Leu Pro Cys Ala Thr Gln Asn Glu Ile 
                325                 330                 335     


Asn Gly Glu Ser Ala Arg Thr Leu Ile Ser Asn Gly Val Lys Ala Ile 
            340                 345                 350         


Gly Glu Gly Ala Asn Met Pro Ser Asp Leu Glu Ala Ile Asn Glu Phe 
        355                 360                 365             


Leu Asn Ala Gly Val Leu Phe Gly Pro Ala Lys Ala Ala Asn Ala Gly 
    370                 375                 380                 


Gly Ala Ala Val Ser Ala Leu Glu Met Ala Gln Asp Ser Ser Arg Val 
385                 390                 395                 400 


Phe Trp Ser Phe Glu Glu Val Asp Ala Lys Leu His Gln Ile Met Lys 
                405                 410                 415     


Asp Ile Tyr Ser Asp Ser Lys Ala Ala Ala Glu Lys Tyr Gly Phe Pro 
            420                 425                 430         


Gly Asn Leu Val Met Gly Ala Asn Ile Ala Gly Phe Ile Lys Val Ala 
        435                 440                 445             


Asp Gly Met Leu Thr Glu Gly Ile Tyr 
    450                 455         


<210>  10
<211>  1371
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LsGluDH-A175G-V386A

<400>  10
atgaccatca ccaccgtttc taacgaacag ctggctaaag aatacgttga cggtgttttc       60

gaacagctga aacagcagaa ctgccaccag gctgaattcc tgcaggctgc tgaagaaatc      120

ttcatctctc tggttccggt tttcgttcag cacccggaat acatcaaagc taacatcctg      180

tctcgtatcg ttgaaccgga ccgtatcatc tctttccgtg ttgcttggca ggacgaccac      240

aaccaggttc aggttaaccg tggttaccgt gttcagtact ctaacgttat gggtccgtac      300

aaaggtggtc tgcgtttcca cccgtctgtt aacgaatcta tcatcaaatt cctgggtttc      360

gaacagatct tcaaaaacgc tctgaccggt cagccgatcg gtggtggtaa aggtggttct      420

aacttcgacc cgaaaggtaa atctgactct gaaatcatgc gtttctgcca ggctttcatg      480

accgaactgt accgtcacat cggtccggac gttgacgttc cgggtggtga catcggtgtt      540

ggtgctcgtg aagttggtta cctgtggggt cagtacaaac gtctgaccaa agcttctgaa      600

tctggtgttc tgaccggtaa aaccccgggt tacggtggtt ctctggctcg taaagaagct      660

accggttacg gtaccgttta cttcgttaac gaaatgctga aagacgttaa cgactctttc      720

gaaggtaaaa ccgttgttgt ttctggttct ggtaacgttt ctacctacgc tatcgaaaaa      780

gctcagcagt acggtgctaa agttgttgct tgctctgact cttctggtta catctacgac      840

ccggaaggtc tggacctgga cgttatcaaa gaaatcaaag aagttaaagg tgaccgtatc      900

tctacctacg tttcttaccg tccgaacgct accttcacca acggttgcac cggtatctgg      960

accatcccgt gcgacatcgc tctgccgtgc gctacccaga acgaaatcaa cggtgaatct     1020

gctcgtaccc tgatctctaa cggtgttaaa gctatcggtg aaggtgctaa catgccgtct     1080

gacctggaag ctatcaacga attcctgaac gctggtgttc tgttcggtcc ggctaaagct     1140

gctaacgctg gtggtgctgc tgtttctgct ctggaaatgg ctcaggactc ttctcgtgtt     1200

ttctggtctt tcgaagaagt tgacgctaaa ctgcaccaga tcatgaaaga catctactct     1260

gactctaaag ctgctgctga aaaatacggt ttcccgggta acctggttat gggtgctaac     1320

atcgctggtt tcatcaaagt tgctgacggt atgctgaccg aaggtatcta c              1371


<210>  11
<211>  449
<212>  PRT
<213>  Pseudomonas putida

<400>  11

Met Ser Thr Met Ile Glu Ser Val Asp Asn Phe Leu Ala Arg Leu Lys 
1               5                   10                  15      


Gln Arg Asp Pro Gly Gln Pro Glu Phe His Gln Ala Val Glu Glu Val 
            20                  25                  30          


Leu Arg Thr Leu Trp Pro Phe Leu Glu Ala Asn Pro His Tyr Leu Gln 
        35                  40                  45              


Ser Gly Ile Leu Glu Arg Met Val Glu Pro Glu Arg Ala Val Leu Phe 
    50                  55                  60                  


Arg Val Ser Trp Val Asp Asp Gln Gly Lys Val Gln Val Asn Arg Gly 
65                  70                  75                  80  


Tyr Arg Ile Gln Met Ser Ser Ala Ile Gly Pro Tyr Lys Gly Gly Leu 
                85                  90                  95      


Arg Phe His Pro Ser Val Asn Leu Ser Val Leu Lys Phe Leu Ala Phe 
            100                 105                 110         


Glu Gln Val Phe Lys Asn Ser Leu Thr Ser Leu Pro Met Gly Gly Gly 
        115                 120                 125             


Lys Gly Gly Ser Asp Phe Asp Pro Lys Gly Lys Ser Asp Ala Glu Val 
    130                 135                 140                 


Met Arg Phe Cys Gln Ala Phe Met Ser Glu Leu Tyr Arg His Ile Gly 
145                 150                 155                 160 


Ala Asp Cys Asp Val Pro Ala Gly Asp Ile Gly Val Gly Ala Arg Glu 
                165                 170                 175     


Ile Gly Phe Met Phe Gly Gln Tyr Lys Arg Leu Ala Asn Gln Phe Thr 
            180                 185                 190         


Ser Val Leu Thr Gly Lys Gly Met Thr Tyr Gly Gly Ser Leu Ile Arg 
        195                 200                 205             


Pro Glu Ala Thr Gly Tyr Gly Cys Val Tyr Phe Ala Glu Glu Met Leu 
    210                 215                 220                 


Lys Arg Gln Asp Lys Arg Ile Asp Gly Arg Arg Val Ala Val Ser Gly 
225                 230                 235                 240 


Ser Gly Asn Val Ala Gln Tyr Ala Ala Arg Lys Val Met Asp Leu Gly 
                245                 250                 255     


Gly Lys Val Ile Ser Leu Ser Asp Ser Glu Gly Thr Leu Tyr Ala Glu 
            260                 265                 270         


Ala Gly Leu Thr Asp Ala Gln Trp Asp Ala Leu Met Glu Leu Lys Asn 
        275                 280                 285             


Val Lys Arg Gly Arg Ile Ser Glu Leu Ala Gly Gln Phe Gly Leu Glu 
    290                 295                 300                 


Phe Arg Lys Gly Gln Thr Pro Trp Ser Leu Pro Cys Asp Ile Ala Leu 
305                 310                 315                 320 


Pro Cys Ala Thr Gln Asn Glu Leu Gly Ala Glu Asp Ala Arg Thr Leu 
                325                 330                 335     


Leu Arg Asn Gly Cys Ile Cys Val Ala Glu Gly Ala Asn Met Pro Thr 
            340                 345                 350         


Thr Leu Glu Ala Val Asp Ile Phe Leu Asp Ala Gly Ile Leu Tyr Ala 
        355                 360                 365             


Pro Gly Lys Ala Ser Asn Ala Gly Gly Val Ala Val Ser Gly Leu Glu 
    370                 375                 380                 


Met Ser Gln Asn Ala Met Arg Leu Leu Trp Thr Ala Gly Glu Val Asp 
385                 390                 395                 400 


Ser Lys Leu His Asn Ile Met Gln Ser Ile His His Ala Cys Val His 
                405                 410                 415     


Tyr Gly Glu Glu Ala Asp Gly Arg Ile Asn Tyr Val Lys Gly Ala Asn 
            420                 425                 430         


Ile Ala Gly Phe Val Lys Val Ala Asp Ala Met Leu Ala Gln Gly Val 
        435                 440                 445             


Val 
    


<210>  12
<211>  1347
<212>  DNA
<213>  Pseudomonas putida

<400>  12
atgtctacca tgatcgaatc tgttgacaac ttcctggctc gtctgaaaca gcgtgacccg       60

ggtcagccgg aattccacca ggctgttgaa gaagttctgc gtaccctgtg gccgttcctg      120

gaagctaacc cgcactacct gcagtctggt atcctggaac gtatggttga accggaacgt      180

gctgttctgt tccgtgtttc ttgggttgac gaccagggta aagttcaggt taaccgtggt      240

taccgtatcc agatgtcttc tgctatcggt ccgtacaaag gtggtctgcg tttccacccg      300

tctgttaacc tgtctgttct gaaattcctg gctttcgaac aggttttcaa aaactctctg      360

acctctctgc cgatgggtgg tggtaaaggt ggttctgact tcgacccgaa aggtaaatct      420

gacgctgaag ttatgcgttt ctgccaggct ttcatgtctg aactgtaccg tcacatcggt      480

gctgactgcg acgttccggc tggtgacatc ggtgttggtg ctcgtgaaat cggtttcatg      540

ttcggtcagt acaaacgtct ggctaaccag ttcacctctg ttctgaccgg taaaggtatg      600

acctacggtg gttctctgat ccgtccggaa gctaccggtt acggttgcgt ttacttcgct      660

gaagaaatgc tgaaacgtca ggacaaacgt atcgacggtc gtcgtgttgc tgtttctggt      720

tctggtaacg ttgctcagta cgctgctcgt aaagttatgg acctgggtgg taaagttatc      780

tctctgtctg actctgaagg taccctgtac gctgaagctg gtctgaccga cgctcagtgg      840

gacgctctga tggaactgaa aaacgttaaa cgtggtcgta tctctgaact ggctggtcag      900

ttcggtctgg aattccgtaa aggtcagacc ccgtggtctc tgccgtgcga catcgctctg      960

ccgtgcgcta cccagaacga actgggtgct gaagacgctc gtaccctgct gcgtaacggt     1020

tgcatctgcg ttgctgaagg tgctaacatg ccgaccaccc tggaagctgt tgacatcttc     1080

ctggacgctg gtatcctgta cgctccgggt aaagcttcta acgctggtgg tgttgctgtt     1140

tctggtctgg aaatgtctca gaacgctatg cgtctgctgt ggaccgctgg tgaagttgac     1200

tctaaactgc acaacatcat gcagtctatc caccacgctt gcgttcacta cggtgaagaa     1260

gctgacggtc gtatcaacta cgttaaaggt gctaacatcg ctggtttcgt taaagttgct     1320

gacgctatgc tggctcaggg tgttgtt                                         1347


<210>  13
<211>  449
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PpGluDH-A167G

<400>  13

Met Ser Thr Met Ile Glu Ser Val Asp Asn Phe Leu Ala Arg Leu Lys 
1               5                   10                  15      


Gln Arg Asp Pro Gly Gln Pro Glu Phe His Gln Ala Val Glu Glu Val 
            20                  25                  30          


Leu Arg Thr Leu Trp Pro Phe Leu Glu Ala Asn Pro His Tyr Leu Gln 
        35                  40                  45              


Ser Gly Ile Leu Glu Arg Met Val Glu Pro Glu Arg Ala Val Leu Phe 
    50                  55                  60                  


Arg Val Ser Trp Val Asp Asp Gln Gly Lys Val Gln Val Asn Arg Gly 
65                  70                  75                  80  


Tyr Arg Ile Gln Met Ser Ser Ala Ile Gly Pro Tyr Lys Gly Gly Leu 
                85                  90                  95      


Arg Phe His Pro Ser Val Asn Leu Ser Val Leu Lys Phe Leu Ala Phe 
            100                 105                 110         


Glu Gln Val Phe Lys Asn Ser Leu Thr Ser Leu Pro Met Gly Gly Gly 
        115                 120                 125             


Lys Gly Gly Ser Asp Phe Asp Pro Lys Gly Lys Ser Asp Ala Glu Val 
    130                 135                 140                 


Met Arg Phe Cys Gln Ala Phe Met Ser Glu Leu Tyr Arg His Ile Gly 
145                 150                 155                 160 


Ala Asp Cys Asp Val Pro Gly Gly Asp Ile Gly Val Gly Ala Arg Glu 
                165                 170                 175     


Ile Gly Phe Met Phe Gly Gln Tyr Lys Arg Leu Ala Asn Gln Phe Thr 
            180                 185                 190         


Ser Val Leu Thr Gly Lys Gly Met Thr Tyr Gly Gly Ser Leu Ile Arg 
        195                 200                 205             


Pro Glu Ala Thr Gly Tyr Gly Cys Val Tyr Phe Ala Glu Glu Met Leu 
    210                 215                 220                 


Lys Arg Gln Asp Lys Arg Ile Asp Gly Arg Arg Val Ala Val Ser Gly 
225                 230                 235                 240 


Ser Gly Asn Val Ala Gln Tyr Ala Ala Arg Lys Val Met Asp Leu Gly 
                245                 250                 255     


Gly Lys Val Ile Ser Leu Ser Asp Ser Glu Gly Thr Leu Tyr Ala Glu 
            260                 265                 270         


Ala Gly Leu Thr Asp Ala Gln Trp Asp Ala Leu Met Glu Leu Lys Asn 
        275                 280                 285             


Val Lys Arg Gly Arg Ile Ser Glu Leu Ala Gly Gln Phe Gly Leu Glu 
    290                 295                 300                 


Phe Arg Lys Gly Gln Thr Pro Trp Ser Leu Pro Cys Asp Ile Ala Leu 
305                 310                 315                 320 


Pro Cys Ala Thr Gln Asn Glu Leu Gly Ala Glu Asp Ala Arg Thr Leu 
                325                 330                 335     


Leu Arg Asn Gly Cys Ile Cys Val Ala Glu Gly Ala Asn Met Pro Thr 
            340                 345                 350         


Thr Leu Glu Ala Val Asp Ile Phe Leu Asp Ala Gly Ile Leu Tyr Ala 
        355                 360                 365             


Pro Gly Lys Ala Ser Asn Ala Gly Gly Val Ala Val Ser Gly Leu Glu 
    370                 375                 380                 


Met Ser Gln Asn Ala Met Arg Leu Leu Trp Thr Ala Gly Glu Val Asp 
385                 390                 395                 400 


Ser Lys Leu His Asn Ile Met Gln Ser Ile His His Ala Cys Val His 
                405                 410                 415     


Tyr Gly Glu Glu Ala Asp Gly Arg Ile Asn Tyr Val Lys Gly Ala Asn 
            420                 425                 430         


Ile Ala Gly Phe Val Lys Val Ala Asp Ala Met Leu Ala Gln Gly Val 
        435                 440                 445             


Val 
    


<210>  14
<211>  1347
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PpGluDH-A167G

<400>  14
atgtctacca tgatcgaatc tgttgacaac ttcctggctc gtctgaaaca gcgtgacccg       60

ggtcagccgg aattccacca ggctgttgaa gaagttctgc gtaccctgtg gccgttcctg      120

gaagctaacc cgcactacct gcagtctggt atcctggaac gtatggttga accggaacgt      180

gctgttctgt tccgtgtttc ttgggttgac gaccagggta aagttcaggt taaccgtggt      240

taccgtatcc agatgtcttc tgctatcggt ccgtacaaag gtggtctgcg tttccacccg      300

tctgttaacc tgtctgttct gaaattcctg gctttcgaac aggttttcaa aaactctctg      360

acctctctgc cgatgggtgg tggtaaaggt ggttctgact tcgacccgaa aggtaaatct      420

gacgctgaag ttatgcgttt ctgccaggct ttcatgtctg aactgtaccg tcacatcggt      480

gctgactgcg acgttccggg tggtgacatc ggtgttggtg ctcgtgaaat cggtttcatg      540

ttcggtcagt acaaacgtct ggctaaccag ttcacctctg ttctgaccgg taaaggtatg      600

acctacggtg gttctctgat ccgtccggaa gctaccggtt acggttgcgt ttacttcgct      660

gaagaaatgc tgaaacgtca ggacaaacgt atcgacggtc gtcgtgttgc tgtttctggt      720

tctggtaacg ttgctcagta cgctgctcgt aaagttatgg acctgggtgg taaagttatc      780

tctctgtctg actctgaagg taccctgtac gctgaagctg gtctgaccga cgctcagtgg      840

gacgctctga tggaactgaa aaacgttaaa cgtggtcgta tctctgaact ggctggtcag      900

ttcggtctgg aattccgtaa aggtcagacc ccgtggtctc tgccgtgcga catcgctctg      960

ccgtgcgcta cccagaacga actgggtgct gaagacgctc gtaccctgct gcgtaacggt     1020

tgcatctgcg ttgctgaagg tgctaacatg ccgaccaccc tggaagctgt tgacatcttc     1080

ctggacgctg gtatcctgta cgctccgggt aaagcttcta acgctggtgg tgttgctgtt     1140

tctggtctgg aaatgtctca gaacgctatg cgtctgctgt ggaccgctgg tgaagttgac     1200

tctaaactgc acaacatcat gcagtctatc caccacgctt gcgttcacta cggtgaagaa     1260

gctgacggtc gtatcaacta cgttaaaggt gctaacatcg ctggtttcgt taaagttgct     1320

gacgctatgc tggctcaggg tgttgtt                                         1347


<210>  15
<211>  449
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PpGluDH-V378A

<400>  15

Met Ser Thr Met Ile Glu Ser Val Asp Asn Phe Leu Ala Arg Leu Lys 
1               5                   10                  15      


Gln Arg Asp Pro Gly Gln Pro Glu Phe His Gln Ala Val Glu Glu Val 
            20                  25                  30          


Leu Arg Thr Leu Trp Pro Phe Leu Glu Ala Asn Pro His Tyr Leu Gln 
        35                  40                  45              


Ser Gly Ile Leu Glu Arg Met Val Glu Pro Glu Arg Ala Val Leu Phe 
    50                  55                  60                  


Arg Val Ser Trp Val Asp Asp Gln Gly Lys Val Gln Val Asn Arg Gly 
65                  70                  75                  80  


Tyr Arg Ile Gln Met Ser Ser Ala Ile Gly Pro Tyr Lys Gly Gly Leu 
                85                  90                  95      


Arg Phe His Pro Ser Val Asn Leu Ser Val Leu Lys Phe Leu Ala Phe 
            100                 105                 110         


Glu Gln Val Phe Lys Asn Ser Leu Thr Ser Leu Pro Met Gly Gly Gly 
        115                 120                 125             


Lys Gly Gly Ser Asp Phe Asp Pro Lys Gly Lys Ser Asp Ala Glu Val 
    130                 135                 140                 


Met Arg Phe Cys Gln Ala Phe Met Ser Glu Leu Tyr Arg His Ile Gly 
145                 150                 155                 160 


Ala Asp Cys Asp Val Pro Ala Gly Asp Ile Gly Val Gly Ala Arg Glu 
                165                 170                 175     


Ile Gly Phe Met Phe Gly Gln Tyr Lys Arg Leu Ala Asn Gln Phe Thr 
            180                 185                 190         


Ser Val Leu Thr Gly Lys Gly Met Thr Tyr Gly Gly Ser Leu Ile Arg 
        195                 200                 205             


Pro Glu Ala Thr Gly Tyr Gly Cys Val Tyr Phe Ala Glu Glu Met Leu 
    210                 215                 220                 


Lys Arg Gln Asp Lys Arg Ile Asp Gly Arg Arg Val Ala Val Ser Gly 
225                 230                 235                 240 


Ser Gly Asn Val Ala Gln Tyr Ala Ala Arg Lys Val Met Asp Leu Gly 
                245                 250                 255     


Gly Lys Val Ile Ser Leu Ser Asp Ser Glu Gly Thr Leu Tyr Ala Glu 
            260                 265                 270         


Ala Gly Leu Thr Asp Ala Gln Trp Asp Ala Leu Met Glu Leu Lys Asn 
        275                 280                 285             


Val Lys Arg Gly Arg Ile Ser Glu Leu Ala Gly Gln Phe Gly Leu Glu 
    290                 295                 300                 


Phe Arg Lys Gly Gln Thr Pro Trp Ser Leu Pro Cys Asp Ile Ala Leu 
305                 310                 315                 320 


Pro Cys Ala Thr Gln Asn Glu Leu Gly Ala Glu Asp Ala Arg Thr Leu 
                325                 330                 335     


Leu Arg Asn Gly Cys Ile Cys Val Ala Glu Gly Ala Asn Met Pro Thr 
            340                 345                 350         


Thr Leu Glu Ala Val Asp Ile Phe Leu Asp Ala Gly Ile Leu Tyr Ala 
        355                 360                 365             


Pro Gly Lys Ala Ser Asn Ala Gly Gly Ala Ala Val Ser Gly Leu Glu 
    370                 375                 380                 


Met Ser Gln Asn Ala Met Arg Leu Leu Trp Thr Ala Gly Glu Val Asp 
385                 390                 395                 400 


Ser Lys Leu His Asn Ile Met Gln Ser Ile His His Ala Cys Val His 
                405                 410                 415     


Tyr Gly Glu Glu Ala Asp Gly Arg Ile Asn Tyr Val Lys Gly Ala Asn 
            420                 425                 430         


Ile Ala Gly Phe Val Lys Val Ala Asp Ala Met Leu Ala Gln Gly Val 
        435                 440                 445             


Val 
    


<210>  16
<211>  1347
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PpGluDH-V378A

<400>  16
atgtctacca tgatcgaatc tgttgacaac ttcctggctc gtctgaaaca gcgtgacccg       60

ggtcagccgg aattccacca ggctgttgaa gaagttctgc gtaccctgtg gccgttcctg      120

gaagctaacc cgcactacct gcagtctggt atcctggaac gtatggttga accggaacgt      180

gctgttctgt tccgtgtttc ttgggttgac gaccagggta aagttcaggt taaccgtggt      240

taccgtatcc agatgtcttc tgctatcggt ccgtacaaag gtggtctgcg tttccacccg      300

tctgttaacc tgtctgttct gaaattcctg gctttcgaac aggttttcaa aaactctctg      360

acctctctgc cgatgggtgg tggtaaaggt ggttctgact tcgacccgaa aggtaaatct      420

gacgctgaag ttatgcgttt ctgccaggct ttcatgtctg aactgtaccg tcacatcggt      480

gctgactgcg acgttccggc tggtgacatc ggtgttggtg ctcgtgaaat cggtttcatg      540

ttcggtcagt acaaacgtct ggctaaccag ttcacctctg ttctgaccgg taaaggtatg      600

acctacggtg gttctctgat ccgtccggaa gctaccggtt acggttgcgt ttacttcgct      660

gaagaaatgc tgaaacgtca ggacaaacgt atcgacggtc gtcgtgttgc tgtttctggt      720

tctggtaacg ttgctcagta cgctgctcgt aaagttatgg acctgggtgg taaagttatc      780

tctctgtctg actctgaagg taccctgtac gctgaagctg gtctgaccga cgctcagtgg      840

gacgctctga tggaactgaa aaacgttaaa cgtggtcgta tctctgaact ggctggtcag      900

ttcggtctgg aattccgtaa aggtcagacc ccgtggtctc tgccgtgcga catcgctctg      960

ccgtgcgcta cccagaacga actgggtgct gaagacgctc gtaccctgct gcgtaacggt     1020

tgcatctgcg ttgctgaagg tgctaacatg ccgaccaccc tggaagctgt tgacatcttc     1080

ctggacgctg gtatcctgta cgctccgggt aaagcttcta acgctggtgg tgctgctgtt     1140

tctggtctgg aaatgtctca gaacgctatg cgtctgctgt ggaccgctgg tgaagttgac     1200

tctaaactgc acaacatcat gcagtctatc caccacgctt gcgttcacta cggtgaagaa     1260

gctgacggtc gtatcaacta cgttaaaggt gctaacatcg ctggtttcgt taaagttgct     1320

gacgctatgc tggctcaggg tgttgtt                                         1347


<210>  17
<211>  449
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PpGluDH-A167G-V378A

<400>  17

Met Ser Thr Met Ile Glu Ser Val Asp Asn Phe Leu Ala Arg Leu Lys 
1               5                   10                  15      


Gln Arg Asp Pro Gly Gln Pro Glu Phe His Gln Ala Val Glu Glu Val 
            20                  25                  30          


Leu Arg Thr Leu Trp Pro Phe Leu Glu Ala Asn Pro His Tyr Leu Gln 
        35                  40                  45              


Ser Gly Ile Leu Glu Arg Met Val Glu Pro Glu Arg Ala Val Leu Phe 
    50                  55                  60                  


Arg Val Ser Trp Val Asp Asp Gln Gly Lys Val Gln Val Asn Arg Gly 
65                  70                  75                  80  


Tyr Arg Ile Gln Met Ser Ser Ala Ile Gly Pro Tyr Lys Gly Gly Leu 
                85                  90                  95      


Arg Phe His Pro Ser Val Asn Leu Ser Val Leu Lys Phe Leu Ala Phe 
            100                 105                 110         


Glu Gln Val Phe Lys Asn Ser Leu Thr Ser Leu Pro Met Gly Gly Gly 
        115                 120                 125             


Lys Gly Gly Ser Asp Phe Asp Pro Lys Gly Lys Ser Asp Ala Glu Val 
    130                 135                 140                 


Met Arg Phe Cys Gln Ala Phe Met Ser Glu Leu Tyr Arg His Ile Gly 
145                 150                 155                 160 


Ala Asp Cys Asp Val Pro Gly Gly Asp Ile Gly Val Gly Ala Arg Glu 
                165                 170                 175     


Ile Gly Phe Met Phe Gly Gln Tyr Lys Arg Leu Ala Asn Gln Phe Thr 
            180                 185                 190         


Ser Val Leu Thr Gly Lys Gly Met Thr Tyr Gly Gly Ser Leu Ile Arg 
        195                 200                 205             


Pro Glu Ala Thr Gly Tyr Gly Cys Val Tyr Phe Ala Glu Glu Met Leu 
    210                 215                 220                 


Lys Arg Gln Asp Lys Arg Ile Asp Gly Arg Arg Val Ala Val Ser Gly 
225                 230                 235                 240 


Ser Gly Asn Val Ala Gln Tyr Ala Ala Arg Lys Val Met Asp Leu Gly 
                245                 250                 255     


Gly Lys Val Ile Ser Leu Ser Asp Ser Glu Gly Thr Leu Tyr Ala Glu 
            260                 265                 270         


Ala Gly Leu Thr Asp Ala Gln Trp Asp Ala Leu Met Glu Leu Lys Asn 
        275                 280                 285             


Val Lys Arg Gly Arg Ile Ser Glu Leu Ala Gly Gln Phe Gly Leu Glu 
    290                 295                 300                 


Phe Arg Lys Gly Gln Thr Pro Trp Ser Leu Pro Cys Asp Ile Ala Leu 
305                 310                 315                 320 


Pro Cys Ala Thr Gln Asn Glu Leu Gly Ala Glu Asp Ala Arg Thr Leu 
                325                 330                 335     


Leu Arg Asn Gly Cys Ile Cys Val Ala Glu Gly Ala Asn Met Pro Thr 
            340                 345                 350         


Thr Leu Glu Ala Val Asp Ile Phe Leu Asp Ala Gly Ile Leu Tyr Ala 
        355                 360                 365             


Pro Gly Lys Ala Ser Asn Ala Gly Gly Ala Ala Val Ser Gly Leu Glu 
    370                 375                 380                 


Met Ser Gln Asn Ala Met Arg Leu Leu Trp Thr Ala Gly Glu Val Asp 
385                 390                 395                 400 


Ser Lys Leu His Asn Ile Met Gln Ser Ile His His Ala Cys Val His 
                405                 410                 415     


Tyr Gly Glu Glu Ala Asp Gly Arg Ile Asn Tyr Val Lys Gly Ala Asn 
            420                 425                 430         


Ile Ala Gly Phe Val Lys Val Ala Asp Ala Met Leu Ala Gln Gly Val 
        435                 440                 445             


Val 
    


<210>  18
<211>  1347
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PpGluDH-A167G-V378A

<400>  18
atgtctacca tgatcgaatc tgttgacaac ttcctggctc gtctgaaaca gcgtgacccg       60

ggtcagccgg aattccacca ggctgttgaa gaagttctgc gtaccctgtg gccgttcctg      120

gaagctaacc cgcactacct gcagtctggt atcctggaac gtatggttga accggaacgt      180

gctgttctgt tccgtgtttc ttgggttgac gaccagggta aagttcaggt taaccgtggt      240

taccgtatcc agatgtcttc tgctatcggt ccgtacaaag gtggtctgcg tttccacccg      300

tctgttaacc tgtctgttct gaaattcctg gctttcgaac aggttttcaa aaactctctg      360

acctctctgc cgatgggtgg tggtaaaggt ggttctgact tcgacccgaa aggtaaatct      420

gacgctgaag ttatgcgttt ctgccaggct ttcatgtctg aactgtaccg tcacatcggt      480

gctgactgcg acgttccggg tggtgacatc ggtgttggtg ctcgtgaaat cggtttcatg      540

ttcggtcagt acaaacgtct ggctaaccag ttcacctctg ttctgaccgg taaaggtatg      600

acctacggtg gttctctgat ccgtccggaa gctaccggtt acggttgcgt ttacttcgct      660

gaagaaatgc tgaaacgtca ggacaaacgt atcgacggtc gtcgtgttgc tgtttctggt      720

tctggtaacg ttgctcagta cgctgctcgt aaagttatgg acctgggtgg taaagttatc      780

tctctgtctg actctgaagg taccctgtac gctgaagctg gtctgaccga cgctcagtgg      840

gacgctctga tggaactgaa aaacgttaaa cgtggtcgta tctctgaact ggctggtcag      900

ttcggtctgg aattccgtaa aggtcagacc ccgtggtctc tgccgtgcga catcgctctg      960

ccgtgcgcta cccagaacga actgggtgct gaagacgctc gtaccctgct gcgtaacggt     1020

tgcatctgcg ttgctgaagg tgctaacatg ccgaccaccc tggaagctgt tgacatcttc     1080

ctggacgctg gtatcctgta cgctccgggt aaagcttcta acgctggtgg tgctgctgtt     1140

tctggtctgg aaatgtctca gaacgctatg cgtctgctgt ggaccgctgg tgaagttgac     1200

tctaaactgc acaacatcat gcagtctatc caccacgctt gcgttcacta cggtgaagaa     1260

gctgacggtc gtatcaacta cgttaaaggt gctaacatcg ctggtttcgt taaagttgct     1320

gacgctatgc tggctcaggg tgttgtt                                         1347


