                         SEQUENCE LISTING

<110>  MODERNATX, INC.
 
<120>  DIFFERENTIALLY EXPRESSED IMMUNE CELL MICRORNAS FOR REGULATION OF 
       PROTEIN EXPRESSION

<130>  MDN-822PC

<140>  PCT/US2020/031885
<141>  2020-05-07

<150>  US 62/844,704
<151>  2019-05-07

<160>  83    

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-150-5p

<400>  1
ucucccaacc cuuguaccag ug                                                22


<210>  2
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-150-5p binding site

<400>  2
cactggtaca agggttggga ga                                                22


<210>  3
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-146b-5p

<400>  3
ugagaacuga auuccauagg cug                                               23


<210>  4
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-146b-5p binding site

<400>  4
cagcctatgg aattcagttc tca                                               23


<210>  5
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR 4286

<400>  5
accccacucc ugguacc                                                      17


<210>  6
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-4286 binding site

<400>  6
ggtaccagga gtggggt                                                      17


<210>  7
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-579-3b

<400>  7
uucauuuggu auaaaccgcg auu                                               23


<210>  8
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-579-3b binding site

<400>  8
aatcgcggtt tataccaaat gaa                                               23


<210>  9
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-4516

<400>  9
gggagaaggg ucggggc                                                      17


<210>  10
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-4516 binding site

<400>  10
gccccgaccc ttctccc                                                      17


<210>  11
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-146a-5p

<400>  11
ugagaacuga auuccauggg uu                                                22


<210>  12
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-146a-5p binding site

<400>  12
aacccatgga attcagttct ca                                                22


<210>  13
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-664b-3p

<400>  13
uucauuugcc ucccagccua ca                                                22


<210>  14
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-664b-3p binding site

<400>  14
tgtaggctgg gaggcaaatg aa                                                22


<210>  15
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-342-3p

<400>  15
ucucacacag aaaucgcacc cgu                                               23


<210>  16
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-342-3p binding site

<400>  16
acgggtgcga tttctgtgtg aga                                               23


<210>  17
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-342-5p

<400>  17
aggggugcua ucugugauug a                                                 21


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-342-5p binding site

<400>  18
tcaatcacag atagcacccc t                                                 21


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-1915-3p

<400>  19
ccccagggcg acgcggcggg                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-1915-3p binding site

<400>  20
cccgccgcgt cgccctgggg                                                   20


<210>  21
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-26b-5p

<400>  21
uucaaguaau ucaggauagg u                                                 21


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-26b-5p binding site

<400>  22
acctatcctg aattacttga a                                                 21


<210>  23
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-18a-5p

<400>  23
uaaggugcau cuagugcaga uag                                               23


<210>  24
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-18a-5p binding site

<400>  24
ctatctgcac tagatgcacc tta                                               23


<210>  25
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-1246

<400>  25
aauggauuuu uggagcagg                                                    19


<210>  26
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-1246 binding site

<400>  26
cctgctccaa aaatccatt                                                    19


<210>  27
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR 126-3p

<400>  27
ucguaccgug aguaauaaug cg                                                22


<210>  28
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR 126-3p binding site

<400>  28
cgcattatta ctcacggtac ga                                                22


<210>  29
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: 2A peptide amino acid

<400>  29

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  30
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 2A peptide nucleotide

<400>  30
ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct       60

ggacct                                                                  66


<210>  31
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 2A peptide nucleotide

<400>  31
tccggactca gatccgggga tctcaaaatt gtcgctcctg tcaaacaaac tcttaacttt       60

gatttactca aactggctgg ggatgtagaa agcaatccag gtccactc                   108


<210>  32
<211>  6
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Kozak

<400>  32
gccrcc                                                                   6


<210>  33
<211>  10
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: V1

<400>  33
ccccggcgcc                                                              10


<210>  34
<211>  7
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: V2

<400>  34
ccccggc                                                                  7


<210>  35
<211>  6
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: EK

<400>  35
gccgcc                                                                   6


<210>  36
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR

<400>  36
gggaaauaag agagaaaaga agaguaagaa gaaauauaag a                           41


<210>  37
<211>  57
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR

<400>  37
gggaaauaag agagaaaaga agaguaagaa gaaauauaag accccggcgc cgccacc          57


<210>  38
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Standard 5' UTR

<400>  38
gggaaauaag agagaaaaga agaguaagaa gaaauauaag agccacc                     47


<210>  39
<211>  57
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: V1-UTR

<400>  39
gggaaauaag agagaaaaga agaguaagaa gaaauauaag accccggcgc cgccacc          57


<210>  40
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: V2-UTR

<400>  40
gggaaauaag agagaaaaga agaguaagaa gaaauauaag accccggcgc cacc             54


<210>  41
<211>  9
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Kozak consensus

<400>  41
ccrccaugg                                                                9


<210>  42
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR-001

<400>  42
gggaaauaag agagaaaaga agaguaagaa gaaauauaag agccacc                     47


<210>  43
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR-002

<400>  43
gggagaucag agagaaaaga agaguaagaa gaaauauaag agccacc                     47


<210>  44
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR-004

<400>  44
gggagacaag cuuggcauuc cgguacuguu gguaaagcca cc                          42


<210>  45
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR-005

<400>  45
gggagaucag agagaaaaga agaguaagaa gaaauauaag agccacc                     47


<210>  46
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR-007

<400>  46
gggagacaag cuuggcauuc cgguacuguu gguaaagcca cc                          42


<210>  47
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR-008

<400>  47
gggaauuaac agagaaaaga agaguaagaa gaaauauaag agccacc                     47


<210>  48
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR-009

<400>  48
gggaaauuag acagaaaaga agaguaagaa gaaauauaag agccacc                     47


<210>  49
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR-010

<400>  49
gggaaauaag agaguaaaga acaguaagaa gaaauauaag agccacc                     47


<210>  50
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR-011

<400>  50
gggaaaaaag agagaaaaga agacuaagaa gaaauauaag agccacc                     47


<210>  51
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR-012

<400>  51
gggaaauaag agagaaaaga agaguaagaa gauauauaag agccacc                     47


<210>  52
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR-013

<400>  52
gggaaauaag agacaaaaca agaguaagaa gaaauauaag agccacc                     47


<210>  53
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR-014

<400>  53
gggaaauuag agaguaaaga acaguaagua gaauuaaaag agccacc                     47


<210>  54
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR-015

<400>  54
gggaaauaag agagaauaga agaguaagaa gaaauauaag agccacc                     47


<210>  55
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR-016

<400>  55
gggaaauaag agagaaaaga agaguaagaa gaaaauuaag agccacc                     47


<210>  56
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR-017

<400>  56
gggaaauaag agagaaaaga agaguaagaa gaaauuuaag agccacc                     47


<210>  57
<211>  92
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR-018

<400>  57
ucaagcuuuu ggacccucgu acagaagcua auacgacuca cuauagggaa auaagagaga       60

aaagaagagu aagaagaaau auaagagcca cc                                     92


<210>  58
<211>  142
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' UTR with miR-142-3p binding site

<400>  58
ugauaauagu ccauaaagua ggaaacacua cagcuggagc cucgguggcc augcuucuug       60

ccccuugggc cuccccccag ccccuccucc ccuuccugca cccguacccc cguggucuuu      120

gaauaaaguc ugagugggcg gc                                               142


<210>  59
<211>  142
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' UTR with miR-142-3p binding site

<400>  59
ugauaauagg cuggagccuc gguggcucca uaaaguagga aacacuacac augcuucuug       60

ccccuugggc cuccccccag ccccuccucc ccuuccugca cccguacccc cguggucuuu      120

gaauaaaguc ugagugggcg gc                                               142


<210>  60
<211>  142
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' UTR with miR-142-3p binding site

<400>  60
ugauaauagg cuggagccuc gguggccaug cuucuugccc cuuccauaaa guaggaaaca       60

cuacaugggc cuccccccag ccccuccucc ccuuccugca cccguacccc cguggucuuu      120

gaauaaaguc ugagugggcg gc                                               142


<210>  61
<211>  142
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' UTR with miR-142-3p binding site

<400>  61
ugauaauagg cuggagccuc gguggccaug cuucuugccc cuugggccuc cccccagucc       60

auaaaguagg aaacacuaca ccccuccucc ccuuccugca cccguacccc cguggucuuu      120

gaauaaaguc ugagugggcg gc                                               142


<210>  62
<211>  142
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' UTR with miR-142-3p binding site

<400>  62
ugauaauagg cuggagccuc gguggccaug cuucuugccc cuugggccuc cccccagccc       60

cuccuccccu ucuccauaaa guaggaaaca cuacacugca cccguacccc cguggucuuu      120

gaauaaaguc ugagugggcg gc                                               142


<210>  63
<211>  142
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' UTR with miR-142-3p binding site

<400>  63
ugauaauagg cuggagccuc gguggccaug cuucuugccc cuugggccuc cccccagccc       60

cuccuccccu uccugcaccc guacccccuc cauaaaguag gaaacacuac aguggucuuu      120

gaauaaaguc ugagugggcg gc                                               142


<210>  64
<211>  142
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' UTR with miR-142-3p binding site

<400>  64
ugauaauagg cuggagccuc gguggccaug cuucuugccc cuugggccuc cccccagccc       60

cuccuccccu uccugcaccc guacccccgu ggucuuugaa uaaaguucca uaaaguagga      120

aacacuacac ugagugggcg gc                                               142


<210>  65
<211>  116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' UTR-018

<400>  65
uaauaggcug gagccucggu ggccaugcuu cuugccccuu gggccucccc ccagccccuc       60

cuccccuucc ugcacccgua cccccguggu cuuugaauaa agucugagug ggcggc          116


<210>  66
<211>  164
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' UTR with miR-142-3p binding site variant 1

<400>  66
ugauaauagu ccauaaagua ggaaacacua cagcuggagc cucgguggcc augcuucuug       60

ccccuugggc cuccccccag ccccuccucc ccuuccugca cccguacccc ccgcauuauu      120

acucacggua cgaguggucu uugaauaaag ucugaguggg cggc                       164


<210>  67
<211>  164
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' UTR with miR-142-3p binding site variant 2

<400>  67
ugauaauagu ccauaaagua ggaaacacua cagcuggagc cucgguggcc uagcuucuug       60

ccccuugggc cuccccccag ccccuccucc ccuuccugca cccguacccc ccgcauuauu      120

acucacggua cgaguggucu uugaauaaag ucugaguggg cggc                       164


<210>  68
<211>  142
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' UTR with miR-142-3p binding site variant 3

<400>  68
ugauaauagg cuggagccuc gguggccuag cuucuugccc cuugggccuc cccccagccc       60

cuccuccccu uccugcaccc guacccccuc cauaaaguag gaaacacuac aguggucuuu      120

gaauaaaguc ugagugggcg gc                                               142


<210>  69
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: T7 promoter sequence


<220>
<221>  misc_feature
<222>  (21)..(29)
<223>  n is a, c, g, or t

<400>  69
taatacgact cactataggg nnnnnnnnn                                         29


<210>  70
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: T7 promoter

<400>  70
taatacgact cactatag                                                     18


<210>  71
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-155-5p binding site

<400>  71
uuaaugcuaa ucgugauagg ggu                                               23


<210>  72
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miR-155-5p binding site

<400>  72
acccctatca cgattagcat taa                                               23


<210>  73
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' UTR

<400>  73
tcaagctttt ggaccctcgt acagaagcta atacgactca ctatagggaa ataagagaga       60

aaagaagagt aagaagaaat ataagagcca cc                                     92


<210>  74
<211>  119
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' UTR

<400>  74
tgataatagg ctggagcctc ggtggccatg cttcttgccc cttgggcctc cccccagccc       60

ctcctcccct tcctgcaccc gtacccccgt ggtctttgaa taaagtctga gtgggcggc       119


<210>  75
<211>  164
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' UTR with miR-122 and miR-142-3p sites

<400>  75
tgataatagg ctggagcctc ggtggccatg cttcttgccc cttgggccca aacaccattg       60

tcacactcca tccccccagc ccctcctccc cttcctccat aaagtaggaa acactacatg      120

cacccgtacc cccgtggtct ttgaataaag tctgagtggg cggc                       164


<210>  76
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: repeating GC-motif


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  This region contains n repeats of "CCG" wherein n = 1 to 10, n= 2
       to 8, n= 3 to 6, or n= 4 to 5; some positions may or may not be 
       present

<400>  76
ccgccgccgc cgccgccgcc gccgccgccg                                        30


<210>  77
<211>  15
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: repeating GC-motif


<220>
<221>  misc_feature
<222>  (1)..(15)
<223>  This region contains n repeats of "CCG" wherein n = 1, 2, 3, 4 or
       5; some positions may or may not be present

<400>  77
ccgccgccgc cgccg                                                        15


<210>  78
<211>  12
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: repeating GC-motif

<400>  78
ccgccgccgc cg                                                           12


<210>  79
<211>  15
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: repeating GC-motif

<400>  79
ccgccgccgc cgccg                                                        15


<210>  80
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: repeating GC-motif


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  This region contains n repeats of "CCG" wherein n = 1 - 10; some 
       positions may or may not be present

<400>  80
ccgccgccgc cgccgccgcc gccgccgccg                                        30


<210>  81
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: repeating GC-motif


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  This region contains n repeats of "GCC" wherein n = 1 - 10; some 
       positions may or may not be present

<400>  81
gccgccgccg ccgccgccgc cgccgccgcc                                        30


<210>  82
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: T7 promoter sequence


<220>
<221>  misc_feature
<222>  (20)..(29)
<223>  n is a, c, g, or t

<400>  82
taatacgact cactataagn nnnnnnnnn                                         29


<210>  83
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: T7 promoter sequence


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (20)..(29)
<223>  n is a, c, g, or t

<400>  83
attatgctga gtgatattcn nnnnnnnnn                                         29


