                         SEQUENCE LISTING

<110>  University of Massachusetts
 
<120>  GENETICALLY ENGINEERED MICROORGANISMS AND METHODS OF USE

<130>  08387-0027WO2

<140>  PCT/US2020/031182
<141>  2020-05-01

<150>  US 62/942,537
<151>  2019-12-02

<150>  US 62/843,149
<151>  2019-05-03

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  1119
<212>  DNA
<213>  Escherichia coli

<400>  1
atgcgaaaac gtattctttt tattggccca ccgctgtacg gtttgttata cccattgatt       60

tctctggctc aggcctttcg tgtaatcgga catgatgtag taattagtag tgctggcaaa      120

ttcgcgaata aagcagcaga agctggactg gttgtttttg atgcagttcc aggtttagat      180

tcagaggctg gatatcgcca tcaggaagag ttgaggaaaa aaagtaatat tattggtcat      240

ttctcttttt ttagcgatga aatggcagat aacctcatcg attttgcagg aaaatggagg      300

ccagatttaa tagtctatcc cccgcttggt ccggcaggcc cattggttgc tgctaaatat      360

agaattcctt cagtgatgct ggctgttgga ttcgcgcata catctgccca tattcagatg      420

ttaaaccgtt ctttaagcaa tgcttacagg cggcatggag tcagcggtcc actatgtgat      480

ttagcatgga ttgatgttgc tcccccaagt atgagcattc ttaaaaatgc tgaagaaccg      540

gttatctcaa tgagatatat tccttataac ggaggtgctg taaaggaaac atggtgggac      600

agggattctg atcgaaaacg tttactcatc agccttggca ctgtaaaacc aatggttgat      660

ggtctggagc tgatttcatg ggttatggat tctgcaaatg aagttgatgc tgatatcatt      720

ttgcaacttg caataaatgc tcgtactgga ttacgaaaac taccatcaaa tgtacgtctg      780

gttgactgga tacctatggg tgtattcctt aatggagctg atggatttat tcatcatggt      840

ggcgcaggta ataccctgac agcgttgtat agtgggatac cacagattgt gtttggcgaa      900

ggtgcagatc gctctgttaa tgcagaaatt gttgcgatgc gtgggtgtgg gattattccg      960

gacaagcatg gactgaccag tgatttggta aatcgcctgc tttatgatga ttcactacgc     1020

ttctgttcag atcaggtagc cgctgaaatg gctgaacaac ccagtcctgc agagatcgca     1080

gaggttttga tgagaaaatt aaaaaacaac gggaaataa                            1119


<210>  2
<211>  1551
<212>  DNA
<213>  Escherichia coli

<400>  2
atgagtcatc agtgttcact ttctgaactg aatgaaaacc tggtgccttt cactgccagg       60

cagatcaagt cctcattaat ctggtgtgca gaggatgtca gaaatccagg cgagctgcaa      120

aatgcctgca gttatattat cgatcctgac agtacggctt ctgccaaagt gttccatgca      180

gagcgctatg gtggcagtgg tattcagcgt aatggaggtg gtgcacgttg tgggtttgat      240

ggtaactacc aggttaaagg aataggaagt aatccgttgg ttggtgaagg tactgacgaa      300

cgtcattcta atggtgcact cggcgctgtt catgcaatat atgaggcttt gtggggagaa      360

gtactggctc aaatattacc ttatagtgct gtgcgggttc gggcggtttt acttacagat      420

ctctatactg aaaaggcatt tgagcgctcc ggtatgaaat cacgaagagc cctgttggta      480

cgtgagcctg ttgttcgccc ggcgcatttt gaacgggcac catacttcca agtaaaaccg      540

gagtattcca gtcagttaat tcacgatgcc tgtcgggtta gatctgtgat ccacaagctg      600

ccaggatatc tacctgtacc accggaagaa attgatgctg aagcacgaac tgatccccgg      660

atttattgca ttgagggatt atgtgaactg gcacgtcgtg aggcctggca aatggcattt      720

tgtcgaacac gtttcctgag attgacaact tctccttcta atattgcaat ggatggcaga      780

ttaatggatt ttaacggact cagttgctcg tttccgggag attccccagc tgattttggg      840

tataaactaa gattagctga actggcaaaa gaaccgatgg tacttatgca agggctgtct      900

gatctctgct tgtatatcgg aaaatatatg tttgaccctg acttcactct tgcagcccgt      960

ttgaaggttg aggagatatt tcagaaaact tttcatgaag catgttatta ctgttatcta     1020

gaactgttgg gtattcctgg agaatttata acacaaaaag agatacctga tatattgaaa     1080

caactggtta acagttttgt tgcattactc aataaatact gcgagaaatc acatgcccaa     1140

gatattgtca atcaggatgg ttcaccattg caaaagttgg ttgtgacgct aatccatcat     1200

aggcataatc aaaagcaggc actgaatagt agcatcaaga atgatgttta tttcaccgtt     1260

gcacaacagt gtttttccca gactatccac tggctgacgc aaggcagtac cagacgtcag     1320

ataaatgctt cattactcct gaaagaaatt gaacatcata ccatgaaaag gctgcaaccc     1380

agggaagagc tgaggaaaga gaatatgtgc gaaaaaattg ccatcctgct ggataatcat     1440

ggcgatgatc cccttttttt acaagaagca atttctgata tgaaaaattt tatgcttaag     1500

ttttccagag atgcatttgg atatcttgaa ccgataagaa acacagtgta a              1551


<210>  3
<211>  453
<212>  DNA
<213>  Escherichia coli

<400>  3
atgtcttata taagggaaac catcagagga aaagatgaat ggactgttta tgaacagata       60

ggttttgcgg tcagttgtat gctctacaat cgtaattaca gtctgtatcc ggtgttaacc      120

attcaatact ggactgaata tgcgatacag cataatcaga ttaaattcct gtttgattca      180

cgaggttttc cactggcgta tataacctgg gcatatcttg aggctgatac ggaagcgcgc      240

ctgctcaggg atccagaatt caggttgcat ccgtctgaat ggaatgaaga tggaaggatc      300

tggatcctgg atttctgttg taaaccaggc tttggtcgaa aagttattga ctatctcata      360

cagcttcagc catgggggga aggagaagta cgatggttaa gcaggcgaaa gaaaattgtg      420

acatacatcc ctgagcggct gcataaaacg tag                                   453


<210>  4
<211>  228
<212>  DNA
<213>  Escherichia coli

<400>  4
atgcgagaaa taacagaatc acagttaaga tatatttccg gggcgggagg tgcgccagcg       60

acttcagcta atgccgcagg tgctgcagct attgttggag ctctcgccgg aatacctggt      120

ggtccacttg gggttgtagt tggagccgta tctgccggtt tgacaacagc aattggctcg      180

accgtgggaa gtggtagtgc cagttcttct gctggtggcg gtagctaa                   228


<210>  5
<211>  1275
<212>  DNA
<213>  Escherichia coli

<400>  5
ttgtttcgtc aggatgcttt agaaaacaga aaaatgaagt ggcagggacg ggcaatatta       60

cttcccggaa taccactatg gttaatcatg ctgggaagca ttgtgtttat tacggcattt      120

ctgatgttca ttattgttgg tacctatagc cgccgtgtta atgtcagtgg tgaggtcaca      180

acctggccaa gagctgtcaa tatatattca ggtgtacagg gatttgttgt caggcaattt      240

gttcatgaag ggcagttgat aaaaaaaggg gatcctgttt atctgattga catcagtaaa      300

agtacacgta gtggtattgt cactgataat catcggcggg atatagaaaa tcagctggtt      360

cgtgtggaca acattatttc ccgtctggaa gaaagtaaaa aaataacgtt agataccctg      420

gaaaaacaac gtctgcaata cacagatgcg tttcgtcgct catcagatat tatacagcgt      480

gcagaggaag ggataaaaat aatgaaaaac aatatggaga attacagaaa ctatcaggca      540

aaagggctga ttaataaaga tcagttaact aaccaggtgg cattatatta tcagcaacaa      600

aacaatcttc tcagcctgag cggacagaac gaacagaatg ccctgcagat aaccactctg      660

gagagtcaga ttcagactca ggctgcagat tttgataacc gtatctacca gatggaactg      720

caacggtacg agttacagaa agaactggtt aacactgatg tggagggcga aattattatc      780

cgggcgttga ctgacgggaa agttgactcc ctgagtgtca ctgtcgggca aatggtcaat      840

accggagaca gccttctgca ggttattcct gagaacattg aaaactatta tcttattctc      900

tgggtcccaa atgatgctgt tccttatatt tcggctggtg acaaagtgaa tattcgttat      960

gaagcctttc cggcagaaaa atttgggcag ttctctgcta cggttaaaac tatatccagg     1020

actcctgcgt caacacagga aatgttgacc tataagggtg caccacagaa tacgccgggc     1080

gcctctgttc cctggtataa agtcattgcg atgcctgaaa agcagattat cagatatgac     1140

gaaaaatacc tccctctgga aaatggaatg aaagccgaaa gtacactatt tctggaaaaa     1200

aggcgtattt accagtggat gctttctcct ttctatgaca tgaaacacag tgcaacagga     1260

ccgctcaatg actaa                                                      1275


<210>  6
<211>  2097
<212>  DNA
<213>  Escherichia coli

<400>  6
atgactaacg ggagtttcag acaaattata aatcagcttg atatgcgctg gcgacgtcgt       60

gttccggtta ttcatcagac ggagaccgct gaatgtggac tggcctgcct ggcaatgata      120

tgcggtcatt ttggtaagaa tattgacctg atatctcttc gccggaagtt taatctctcg      180

gcccgtggag caaaccttgc aggaatcaat ggaatagcgg agcagctggg gatggtcacc      240

cgggctcttt cactggagct ggatgaactt ggtgccctca aaatgccgtg tattctccac      300

tgggatttca gtcactttgt cgtgctggtc agcgtaaagc gtaaccgtta tgtactgcat      360

gatccggcca gaggcagaag atatctcggt cgggaggaaa tgagccggta ttttacgggc      420

attgcacttg aggtctggcc tggaagtgaa ttcctggcgg aaacccagca gatccgcata      480

agtctccgtt cactgattaa cagtatttac ggtattaaaa gaacactggc gaaaattttc      540

tgtctgtcag ttgtaattga agcaatcaat ctggtaatgc cggtggggac tcagctggtt      600

atggatcatg cgattccggc gggggacaga gggctgctga cgcttatttc tgctggcctg      660

atgttcttta tattgctcag ggccgcggtg agtatgctgc gtgcatggtc ctcactggtt      720

atgagcacgc tcatcaatat acagtggcag tcgggtctgt ttaaccatct tctcagactg      780

ccgctggcgt tttttgaacg ccgtaaatta ggtgatatcc agtcgcgttt tggctccctt      840

gacactttga gggccacctt taccacctgt gtggttgggg caatcatgga cagtattatg      900

gttgtggggg tttttgtgat gatgctgtta tatggaggat atcttacctg gatagtgctc      960

ggttttacca tggtttacgt tcttattcgt ctggtgacat acggctatta ccggcaaata     1020

tcggaagaaa ctcttgtcag gggggcccgg gccagctcct attttatgga aagcctgtat     1080

ggtattgcca cggtaaaaat ccaaggtatg gctgggatcc ggggaacaca ctggcttaac     1140

ctgaaaatag atgcgatcaa ttcaggtatt aagttaacca agatggattt gctcttcggg     1200

gggataaata cttttgttgc cgcctgtgat caggtggcga ttttatggct gggtgcaagc     1260

cttgtgatcg ataatcagat gacaataggg atgtttgtgg catttggttc ttttcgtggg     1320

cagttttcgg atcgggttgc ttcgctgacc agttttcttc ttcaactgag aataatgagt     1380

ctgcataatg agcgcattgc agatattgca ctacatgaaa aggaagaaaa gaaaccggaa     1440

attgaaatcg ttgctgacat gagcccggtt tcactggaaa ccactgattt aagctaccgg     1500

tatgacagcc agtcagcaca ggtattcagt ggtctgaatt tgtctgtggc tccgggagaa     1560

agtgtggcta taactggtgc ctccggtgcc ggaaaaacca cattaatgaa agtattatgt     1620

ggactgtttg aaccagatag tggaaaagta ctggttaatg gcacggatat acgtcaactt     1680

ggaataaata attatcaccg tatgatagcc tgtgttatgc aggacgaccg gctattttca     1740

ggatcaattc gtgaaaatat ctgtgggttt gcagaagaaa cagacgacga atggatgaca     1800

gaatgtgcca gagcaagtca tattcatgat gtgataatga aaatgccaat ggggtatgaa     1860

acgttaatag gtgaactggg ggaaggtctt tccggcggtc aaaaacagcg tatattcatt     1920

gcccgagctt tataccggaa acctggaata ttatttatgg atgaggctac aagttctctt     1980

gatacagaaa gtgaacgttt cgtgaatgct gccataaaaa aaatgaatat cacccgggtg     2040

attattgcac acagagaaac tacgttgaga actgttgaca ggattatttc tatttaa        2097


<210>  7
<211>  210
<212>  DNA
<213>  Escherichia coli

<400>  7
atgagttata aaaaactgta ccaattgacg gctatattta gtttacctct tactatctta       60

ttggtttcac tttcatccct tcggattgtt ggcgaaggga attcttatgt tgacgttttt      120

ctaagcttta taatatttct tggttttatt gagctgattc atgggattcg aaagattttg      180

gtctggtcag gctggaaaaa cggaagttaa                                       210


<210>  8
<211>  120
<212>  DNA
<213>  Escherichia coli

<400>  8
atggaatttg ctacaaacag ggttactgta aatgacagtc ggtcagcact gtcatcaact       60

ttgctgttgt ctttgatcat gagcgccact ctactggaat attctttatc gatgacctga      120


<210>  9
<211>  1266
<212>  DNA
<213>  Escherichia coli

<400>  9
atgaaaaact atcttttcca gactcccgaa gatatttgtg tacagttaaa aaaaatgaca       60

catcctgtca caataagaac aacagatatt gctaatttct ggcactatct tgagtcagca      120

actcttccgg tgatcacaaa aagcaccact acagaaaatc gggaggttac atttctgtgg      180

cgctcagaga aagcagtgca aggcgtatat cttcgcctga atcgtgttac agataaaaaa      240

gatgtcaaaa aaggactaat gactcatatc ccttcgacag atatctggat gctgacactg      300

gtgttaccag cttcatatcg gggctcatac tcatttatag aaattcccac agatatgaca      360

caaaaagaca tatttcaact aggaagtcgc ttctctccat tacccggtaa atctgatcca      420

tttaacaaaa cagcagaaat aaatatacga ggattcggag aatcagtcct ttctcttgat      480

atggctcctg aacaaaagga atgggatgat acttcccata aatgtacagg tattctttca      540

acattacatt cctttgttgc aggatatcaa cgccggattc gtttatattt tccccagaat      600

ccaacatcag tacctcttgg attacttgtg ttacctgatg ctgaaatatg gtttgaccgg      660

atggatatta cccgggcatt agatatggcc attaccactg gtcatattgc gccaatggca      720

attatgggga tagacaatat taatgaatct gatcgtatga atatactggg aggcaataaa      780

gaacttatct ttgatatagc ggaaaatctg ataccccagt tatacagaga ctacccgaat      840

atcgtatggg ctggtcgttc taatactata ctggccggtc agagcctcgg tggagtgaca      900

gcactgatgg cagctatata tgcgtcgaca acatttggta caatcattag ccactcacct      960

tcaatgtggt ggaaccctga ccagggcagc ccgattttgt ttactgagaa tgatatctcc     1020

tgggtaagtg agcagatact ttcagcgcct ccgaaagatg taaatatcca acttggagtc     1080

ggttctttag aaggtacaac cgtctcacat gttcagcggt tgcatcagtc gttaatcgca     1140

gcaggtttgg aaagtaacct cactgtctat gccggtggtc atgattatgc ctggtggcgc     1200

ggagcaatta ttgatgcatt agcaaattat aattgcagga agatatcaga taataacttt     1260

gtgtaa                                                                1266


<210>  10
<211>  246
<212>  DNA
<213>  Escherichia coli

<400>  10
atgaattgtg ataataatca cagaaatgaa gaattcattg ttacctttga taaaggcaac       60

aagcaagaca attcaagacg aaaacacgat aattttccta tagaggtaga atcctccgta      120

gagctggaga cacactgtat cacaaataat aagtcggctt ccggtatagt aacacatgac      180

tatgatgccg attatatttg tggttgtggt gaaattatgt gtcctggttg cggtcatgac      240

ctataa                                                                 246


<210>  11
<211>  234
<212>  DNA
<213>  Escherichia coli

<400>  11
atgagagaaa tatcagataa catgcttgat tccgtgaaag gagggatgaa tcttaatgga       60

ttacctgctt ctactaatgt aatagatcta cgtggaaaag atatgggaac atatattgat      120

gctaatggag catgctgggc tccggatact ccatccatca tcatgtatcc ggggggaagt      180

ggaccttctt atagtatgag tagttccaca tccagtgcaa acagcggcag ttaa            234


<210>  12
<211>  435
<212>  DNA
<213>  Escherichia coli

<400>  12
atgtatctta cgaaaaagat tataataagt atgatgttta tattaccatc tgctgcattt       60

tcatcagatc cacctcccct tcaacaatcg ttagaaaaaa caacctattt ttctataggt      120

atgaatgggt ttataggcta tcagagcgaa ggggaaaaat tatacacaca cattcttaca      180

ttagataatc ccgaagagat atttaaaaat ataataaaaa atagaaagtc aactaaggag      240

tctaaaattt atgctgcttg tgggctatat tatttaaacg tagaaaatat agagtcattg      300

tttaatgaaa atgataaaca agaatatgtg tctgtcttaa gaggggatat tttaacaaaa      360

ataaaactga atgatattct gaattctgtg ataataaatg gttgcaacac caaattaata      420

tctgaacata aatga                                                       435


<210>  13
<211>  122
<212>  DNA
<213>  Artificial

<220>
<223>  pBAD promoter sequence

<400>  13
ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc       60

attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg      120

aa                                                                     122


<210>  14
<211>  163
<212>  DNA
<213>  Artificial

<220>
<223>  Pttr promoter sequence

<400>  14
cccaatatcc ctgtcaatta tgttgtttta gatcaacaac aagccgggta tgtggttaac       60

cacaatagag cgcaccccgc ctcgattttt acactgtaaa tcatcgacat tttttattca      120

ttacacatga accaacatcg tgacaaatgt ttcattgttg gca                        163


<210>  15
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  J23119 promoter sequence

<400>  15
ttgacagcta gctcagtcct aggtataatg ctag                                   34


