                         SEQUENCE LISTING

<110>  Agency for Science, Technology and Research
       ImmunoSCAPE Pte. Ltd.
 
<120>  High Yield Production and Use of Enzymatic-Exchangeable Peptide 
       Major Histrocompatibility Complex Class I Single Chain Trimer 
       Tetramer

<130>  SP100642WO

<150>  SG10201904052S
<151>  2019-05-06

<160>  54    

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mellitin secretion leader

<400>  1

Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      


Ser Tyr Ile Tyr Ala 
            20      


<210>  2
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Enterokinase cleavable linker

<400>  2

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 
1               5                   10                  15      


Asp Asp Asp Lys Gly 
            20      


<210>  3
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GS-Linker

<400>  3

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  4
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide ligand 1

<400>  4

Ala Val Phe Ala Ala Ala Ser Asp Ala Lys 
1               5                   10  


<210>  5
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide ligand 2

<400>  5

Ala Val Phe Asp Arg Lys Ser Asp Ala Lys 
1               5                   10  


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide ligand 3

<400>  6

Lys Ile Leu Gly Arg Val Phe Phe Val 
1               5                   


<210>  7
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide ligand 4

<400>  7

Lys Leu Ala Glu Ala Ile Phe Lys Leu 
1               5                   


<210>  8
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide ligand 5

<400>  8

Tyr Ala Glu Thr Ala Ala Phe Ala Tyr 
1               5                   


<210>  9
<211>  99
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  beta-2 microglobulin

<400>  9

Ile Gln Arg Thr Pro Lys Ile Gln Val Tyr Ser Arg His Pro Ala Glu 
1               5                   10                  15      


Asn Gly Lys Ser Asn Phe Leu Asn Cys Tyr Val Ser Gly Phe His Pro 
            20                  25                  30          


Ser Asp Ile Glu Val Asp Leu Leu Lys Asn Gly Glu Arg Ile Glu Lys 
        35                  40                  45              


Val Glu His Ser Asp Leu Ser Phe Ser Lys Asp Trp Ser Phe Tyr Leu 
    50                  55                  60                  


Leu Tyr Tyr Thr Glu Phe Thr Pro Thr Glu Lys Asp Glu Tyr Ala Cys 
65                  70                  75                  80  


Arg Val Asn His Val Thr Leu Ser Gln Pro Lys Ile Val Lys Trp Asp 
                85                  90                  95      


Arg Asp Met 
            


<210>  10
<211>  273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A*11:01 Heavy chain

<400>  10

His Ser Met Arg Tyr Phe Tyr Thr Ser Val Ser Arg Pro Gly Arg Gly 
1               5                   10                  15      


Glu Pro Arg Phe Ile Ala Val Gly Tyr Val Asp Asp Thr Gln Phe Val 
            20                  25                  30          


Arg Phe Asp Ser Asp Ala Ala Ser Gln Arg Met Glu Pro Arg Ala Pro 
        35                  40                  45              


Trp Ile Glu Gln Glu Gly Pro Glu Tyr Trp Asp Gln Glu Thr Arg Asn 
    50                  55                  60                  


Val Lys Ala Gln Ser Gln Thr Asp Arg Val Asp Leu Gly Thr Leu Arg 
65                  70                  75                  80  


Gly Tyr Tyr Asn Gln Ser Glu Asp Gly Ser His Thr Ile Gln Ile Met 
                85                  90                  95      


Tyr Gly Cys Asp Val Gly Pro Asp Gly Arg Phe Leu Arg Gly Tyr Arg 
            100                 105                 110         


Gln Asp Ala Tyr Asp Gly Lys Asp Tyr Ile Ala Leu Asn Glu Asp Leu 
        115                 120                 125             


Arg Ser Trp Thr Ala Ala Asp Met Ala Ala Gln Ile Thr Lys Arg Lys 
    130                 135                 140                 


Trp Glu Ala Ala His Ala Ala Glu Gln Gln Arg Ala Tyr Leu Glu Gly 
145                 150                 155                 160 


Arg Cys Val Glu Trp Leu Arg Arg Tyr Leu Glu Asn Gly Lys Glu Thr 
                165                 170                 175     


Leu Gln Arg Thr Asp Pro Pro Lys Thr His Met Thr His His Pro Ile 
            180                 185                 190         


Ser Asp His Glu Ala Thr Leu Arg Cys Trp Ala Leu Gly Phe Tyr Pro 
        195                 200                 205             


Ala Glu Ile Thr Leu Thr Trp Gln Arg Asp Gly Glu Asp Gln Thr Gln 
    210                 215                 220                 


Asp Thr Glu Leu Val Glu Thr Arg Pro Ala Gly Asp Gly Thr Phe Gln 
225                 230                 235                 240 


Lys Trp Ala Ala Val Val Val Pro Ser Gly Glu Glu Gln Arg Tyr Thr 
                245                 250                 255     


Cys His Val Gln His Glu Gly Leu Pro Lys Pro Leu Thr Leu Arg Trp 
            260                 265                 270         


Glu 
    


<210>  11
<211>  273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A*02:01 Heavy chain

<400>  11

His Ser Met Arg Tyr Phe Phe Thr Ser Val Ser Arg Pro Gly Arg Gly 
1               5                   10                  15      


Glu Pro Arg Phe Ile Ala Val Gly Tyr Val Asp Asp Thr Gln Phe Val 
            20                  25                  30          


Arg Phe Asp Ser Asp Ala Ala Ser Gln Arg Met Glu Pro Arg Ala Pro 
        35                  40                  45              


Trp Ile Glu Gln Glu Gly Pro Glu Tyr Trp Asp Gly Glu Thr Arg Lys 
    50                  55                  60                  


Val Lys Ala His Ser Gln Thr His Arg Val Asp Leu Gly Thr Leu Arg 
65                  70                  75                  80  


Gly Tyr Tyr Asn Gln Ser Glu Ala Gly Ser His Thr Val Gln Arg Met 
                85                  90                  95      


Tyr Gly Cys Asp Val Gly Ser Asp Trp Arg Phe Leu Arg Gly Tyr His 
            100                 105                 110         


Gln Tyr Ala Tyr Asp Gly Lys Asp Tyr Ile Ala Leu Lys Glu Asp Leu 
        115                 120                 125             


Arg Ser Trp Thr Ala Ala Asp Met Ala Ala Gln Thr Thr Lys His Lys 
    130                 135                 140                 


Trp Glu Ala Ala His Val Ala Glu Gln Leu Arg Ala Tyr Leu Glu Gly 
145                 150                 155                 160 


Thr Cys Val Glu Trp Leu Arg Arg Tyr Leu Glu Asn Gly Lys Glu Thr 
                165                 170                 175     


Leu Gln Arg Thr Asp Ala Pro Lys Thr His Met Thr His His Ala Val 
            180                 185                 190         


Ser Asp His Glu Ala Thr Leu Arg Cys Trp Ala Leu Ser Phe Tyr Pro 
        195                 200                 205             


Ala Glu Ile Thr Leu Thr Trp Gln Arg Asp Gly Glu Asp Gln Thr Gln 
    210                 215                 220                 


Asp Thr Glu Leu Val Glu Thr Arg Pro Ala Gly Asp Gly Thr Phe Gln 
225                 230                 235                 240 


Lys Trp Ala Ala Val Val Val Pro Ser Gly Gln Glu Gln Arg Tyr Thr 
                245                 250                 255     


Cys His Val Gln His Glu Gly Leu Pro Lys Pro Leu Thr Leu Arg Trp 
            260                 265                 270         


Glu 
    


<210>  12
<211>  274
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MHC-I heavy chain polypeptide

<400>  12

His Ser Met Arg Tyr Phe Phe Thr Ser Val Ser Arg Pro Gly Arg Gly 
1               5                   10                  15      


Glu Pro Arg Phe Ile Ala Val Gly Tyr Val Asp Asp Thr Gln Phe Val 
            20                  25                  30          


Arg Phe Asp Ser Asp Ala Ala Ser Gln Lys Met Glu Pro Arg Ala Pro 
        35                  40                  45              


Trp Ile Glu Gln Glu Gly Pro Glu Tyr Trp Asp Gln Glu Thr Arg Asn 
    50                  55                  60                  


Met Lys Ala His Ser Gln Thr Asp Arg Ala Asn Leu Gly Thr Leu Arg 
65                  70                  75                  80  


Gly Tyr Tyr Asn Gln Ser Glu Asp Gly Ser His Thr Ile Gln Ile Met 
                85                  90                  95      


Tyr Gly Cys Asp Val Gly Pro Asp Gly Arg Phe Leu Arg Gly Tyr Arg 
            100                 105                 110         


Gln Asp Ala Tyr Asp Gly Lys Asp Tyr Ile Ala Leu Asn Glu Asp Leu 
        115                 120                 125             


Arg Ser Trp Thr Ala Ala Asp Met Ala Ala Gln Ile Thr Lys Arg Lys 
    130                 135                 140                 


Trp Glu Ala Val His Ala Ala Glu Gln Arg Arg Val Tyr Leu Glu Gly 
145                 150                 155                 160 


Arg Cys Val Asp Gly Leu Arg Arg Tyr Leu Glu Asn Gly Lys Glu Thr 
                165                 170                 175     


Leu Gln Arg Thr Asp Pro Pro Lys Thr His Met Thr His His Pro Ile 
            180                 185                 190         


Ser Asp His Glu Ala Thr Leu Arg Cys Trp Ala Leu Gly Phe Tyr Pro 
        195                 200                 205             


Ala Glu Ile Thr Leu Thr Trp Gln Arg Asp Gly Glu Asp Gln Thr Gln 
    210                 215                 220                 


Asp Thr Glu Leu Val Glu Thr Arg Pro Ala Gly Asp Gly Thr Phe Gln 
225                 230                 235                 240 


Lys Trp Ala Ala Val Val Val Pro Ser Gly Glu Glu Gln Arg Tyr Thr 
                245                 250                 255     


Cys His Val Gln His Glu Gly Leu Pro Lys Pro Leu Thr Leu Arg Trp 
            260                 265                 270         


Glu Leu 
        


<210>  13
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BirA recognition site

<400>  13

Leu His His Ile Leu Asp Ala Gln Lys Met Val Trp Asn His Arg 
1               5                   10                  15  


<210>  14
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His6x

<400>  14

His His His His His His 
1               5       


<210>  15
<211>  468
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A*11:01-AVFAAASDAK

<400>  15

Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      


Ser Tyr Ile Tyr Ala Ala Val Phe Ala Ala Ala Ser Asp Ala Lys Gly 
            20                  25                  30          


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Asp 
        35                  40                  45              


Asp Asp Lys Gly Ile Gln Arg Thr Pro Lys Ile Gln Val Tyr Ser Arg 
    50                  55                  60                  


His Pro Ala Glu Asn Gly Lys Ser Asn Phe Leu Asn Cys Tyr Val Ser 
65                  70                  75                  80  


Gly Phe His Pro Ser Asp Ile Glu Val Asp Leu Leu Lys Asn Gly Glu 
                85                  90                  95      


Arg Ile Glu Lys Val Glu His Ser Asp Leu Ser Phe Ser Lys Asp Trp 
            100                 105                 110         


Ser Phe Tyr Leu Leu Tyr Tyr Thr Glu Phe Thr Pro Thr Glu Lys Asp 
        115                 120                 125             


Glu Tyr Ala Cys Arg Val Asn His Val Thr Leu Ser Gln Pro Lys Ile 
    130                 135                 140                 


Val Lys Trp Asp Arg Asp Met Gly Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser His Ser Met Arg Tyr 
                165                 170                 175     


Phe Tyr Thr Ser Val Ser Arg Pro Gly Arg Gly Glu Pro Arg Phe Ile 
            180                 185                 190         


Ala Val Gly Tyr Val Asp Asp Thr Gln Phe Val Arg Phe Asp Ser Asp 
        195                 200                 205             


Ala Ala Ser Gln Arg Met Glu Pro Arg Ala Pro Trp Ile Glu Gln Glu 
    210                 215                 220                 


Gly Pro Glu Tyr Trp Asp Gln Glu Thr Arg Asn Val Lys Ala Gln Ser 
225                 230                 235                 240 


Gln Thr Asp Arg Val Asp Leu Gly Thr Leu Arg Gly Tyr Tyr Asn Gln 
                245                 250                 255     


Ser Glu Asp Gly Ser His Thr Ile Gln Ile Met Tyr Gly Cys Asp Val 
            260                 265                 270         


Gly Pro Asp Gly Arg Phe Leu Arg Gly Tyr Arg Gln Asp Ala Tyr Asp 
        275                 280                 285             


Gly Lys Asp Tyr Ile Ala Leu Asn Glu Asp Leu Arg Ser Trp Thr Ala 
    290                 295                 300                 


Ala Asp Met Ala Ala Gln Ile Thr Lys Arg Lys Trp Glu Ala Ala His 
305                 310                 315                 320 


Ala Ala Glu Gln Gln Arg Ala Tyr Leu Glu Gly Arg Cys Val Glu Trp 
                325                 330                 335     


Leu Arg Arg Tyr Leu Glu Asn Gly Lys Glu Thr Leu Gln Arg Thr Asp 
            340                 345                 350         


Pro Pro Lys Thr His Met Thr His His Pro Ile Ser Asp His Glu Ala 
        355                 360                 365             


Thr Leu Arg Cys Trp Ala Leu Gly Phe Tyr Pro Ala Glu Ile Thr Leu 
    370                 375                 380                 


Thr Trp Gln Arg Asp Gly Glu Asp Gln Thr Gln Asp Thr Glu Leu Val 
385                 390                 395                 400 


Glu Thr Arg Pro Ala Gly Asp Gly Thr Phe Gln Lys Trp Ala Ala Val 
                405                 410                 415     


Val Val Pro Ser Gly Glu Glu Gln Arg Tyr Thr Cys His Val Gln His 
            420                 425                 430         


Glu Gly Leu Pro Lys Pro Leu Thr Leu Arg Trp Glu Pro Gly Ser Leu 
        435                 440                 445             


His His Ile Leu Asp Ala Gln Lys Met Val Trp Asn His Arg His His 
    450                 455                 460                 


His His His His 
465             


<210>  16
<211>  468
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A*11:01-AVFDRKSDAK

<400>  16

Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      


Ser Tyr Ile Tyr Ala Ala Val Phe Asp Arg Lys Ser Asp Ala Lys Gly 
            20                  25                  30          


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Asp 
        35                  40                  45              


Asp Asp Lys Gly Ile Gln Arg Thr Pro Lys Ile Gln Val Tyr Ser Arg 
    50                  55                  60                  


His Pro Ala Glu Asn Gly Lys Ser Asn Phe Leu Asn Cys Tyr Val Ser 
65                  70                  75                  80  


Gly Phe His Pro Ser Asp Ile Glu Val Asp Leu Leu Lys Asn Gly Glu 
                85                  90                  95      


Arg Ile Glu Lys Val Glu His Ser Asp Leu Ser Phe Ser Lys Asp Trp 
            100                 105                 110         


Ser Phe Tyr Leu Leu Tyr Tyr Thr Glu Phe Thr Pro Thr Glu Lys Asp 
        115                 120                 125             


Glu Tyr Ala Cys Arg Val Asn His Val Thr Leu Ser Gln Pro Lys Ile 
    130                 135                 140                 


Val Lys Trp Asp Arg Asp Met Gly Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser His Ser Met Arg Tyr 
                165                 170                 175     


Phe Tyr Thr Ser Val Ser Arg Pro Gly Arg Gly Glu Pro Arg Phe Ile 
            180                 185                 190         


Ala Val Gly Tyr Val Asp Asp Thr Gln Phe Val Arg Phe Asp Ser Asp 
        195                 200                 205             


Ala Ala Ser Gln Arg Met Glu Pro Arg Ala Pro Trp Ile Glu Gln Glu 
    210                 215                 220                 


Gly Pro Glu Tyr Trp Asp Gln Glu Thr Arg Asn Val Lys Ala Gln Ser 
225                 230                 235                 240 


Gln Thr Asp Arg Val Asp Leu Gly Thr Leu Arg Gly Tyr Tyr Asn Gln 
                245                 250                 255     


Ser Glu Asp Gly Ser His Thr Ile Gln Ile Met Tyr Gly Cys Asp Val 
            260                 265                 270         


Gly Pro Asp Gly Arg Phe Leu Arg Gly Tyr Arg Gln Asp Ala Tyr Asp 
        275                 280                 285             


Gly Lys Asp Tyr Ile Ala Leu Asn Glu Asp Leu Arg Ser Trp Thr Ala 
    290                 295                 300                 


Ala Asp Met Ala Ala Gln Ile Thr Lys Arg Lys Trp Glu Ala Ala His 
305                 310                 315                 320 


Ala Ala Glu Gln Gln Arg Ala Tyr Leu Glu Gly Arg Cys Val Glu Trp 
                325                 330                 335     


Leu Arg Arg Tyr Leu Glu Asn Gly Lys Glu Thr Leu Gln Arg Thr Asp 
            340                 345                 350         


Pro Pro Lys Thr His Met Thr His His Pro Ile Ser Asp His Glu Ala 
        355                 360                 365             


Thr Leu Arg Cys Trp Ala Leu Gly Phe Tyr Pro Ala Glu Ile Thr Leu 
    370                 375                 380                 


Thr Trp Gln Arg Asp Gly Glu Asp Gln Thr Gln Asp Thr Glu Leu Val 
385                 390                 395                 400 


Glu Thr Arg Pro Ala Gly Asp Gly Thr Phe Gln Lys Trp Ala Ala Val 
                405                 410                 415     


Val Val Pro Ser Gly Glu Glu Gln Arg Tyr Thr Cys His Val Gln His 
            420                 425                 430         


Glu Gly Leu Pro Lys Pro Leu Thr Leu Arg Trp Glu Pro Gly Ser Leu 
        435                 440                 445             


His His Ile Leu Asp Ala Gln Lys Met Val Trp Asn His Arg His His 
    450                 455                 460                 


His His His His 
465             


<210>  17
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A*02:01-KILGRVFFV

<400>  17

Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      


Ser Tyr Ile Tyr Ala Lys Ile Leu Gly Arg Val Phe Phe Val Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Asp Asp 
        35                  40                  45              


Asp Lys Gly Ile Gln Arg Thr Pro Lys Ile Gln Val Tyr Ser Arg His 
    50                  55                  60                  


Pro Ala Glu Asn Gly Lys Ser Asn Phe Leu Asn Cys Tyr Val Ser Gly 
65                  70                  75                  80  


Phe His Pro Ser Asp Ile Glu Val Asp Leu Leu Lys Asn Gly Glu Arg 
                85                  90                  95      


Ile Glu Lys Val Glu His Ser Asp Leu Ser Phe Ser Lys Asp Trp Ser 
            100                 105                 110         


Phe Tyr Leu Leu Tyr Tyr Thr Glu Phe Thr Pro Thr Glu Lys Asp Glu 
        115                 120                 125             


Tyr Ala Cys Arg Val Asn His Val Thr Leu Ser Gln Pro Lys Ile Val 
    130                 135                 140                 


Lys Trp Asp Arg Asp Met Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser His Ser Met Arg Tyr Phe 
                165                 170                 175     


Phe Thr Ser Val Ser Arg Pro Gly Arg Gly Glu Pro Arg Phe Ile Ala 
            180                 185                 190         


Val Gly Tyr Val Asp Asp Thr Gln Phe Val Arg Phe Asp Ser Asp Ala 
        195                 200                 205             


Ala Ser Gln Arg Met Glu Pro Arg Ala Pro Trp Ile Glu Gln Glu Gly 
    210                 215                 220                 


Pro Glu Tyr Trp Asp Gly Glu Thr Arg Lys Val Lys Ala His Ser Gln 
225                 230                 235                 240 


Thr His Arg Val Asp Leu Gly Thr Leu Arg Gly Tyr Tyr Asn Gln Ser 
                245                 250                 255     


Glu Ala Gly Ser His Thr Val Gln Arg Met Tyr Gly Cys Asp Val Gly 
            260                 265                 270         


Ser Asp Trp Arg Phe Leu Arg Gly Tyr His Gln Tyr Ala Tyr Asp Gly 
        275                 280                 285             


Lys Asp Tyr Ile Ala Leu Lys Glu Asp Leu Arg Ser Trp Thr Ala Ala 
    290                 295                 300                 


Asp Met Ala Ala Gln Thr Thr Lys His Lys Trp Glu Ala Ala His Val 
305                 310                 315                 320 


Ala Glu Gln Leu Arg Ala Tyr Leu Glu Gly Thr Cys Val Glu Trp Leu 
                325                 330                 335     


Arg Arg Tyr Leu Glu Asn Gly Lys Glu Thr Leu Gln Arg Thr Asp Ala 
            340                 345                 350         


Pro Lys Thr His Met Thr His His Ala Val Ser Asp His Glu Ala Thr 
        355                 360                 365             


Leu Arg Cys Trp Ala Leu Ser Phe Tyr Pro Ala Glu Ile Thr Leu Thr 
    370                 375                 380                 


Trp Gln Arg Asp Gly Glu Asp Gln Thr Gln Asp Thr Glu Leu Val Glu 
385                 390                 395                 400 


Thr Arg Pro Ala Gly Asp Gly Thr Phe Gln Lys Trp Ala Ala Val Val 
                405                 410                 415     


Val Pro Ser Gly Gln Glu Gln Arg Tyr Thr Cys His Val Gln His Glu 
            420                 425                 430         


Gly Leu Pro Lys Pro Leu Thr Leu Arg Trp Glu Pro Gly Ser Leu His 
        435                 440                 445             


His Ile Leu Asp Ala Gln Lys Met Val Trp Asn His Arg His His His 
    450                 455                 460                 


His His 
465     


<210>  18
<211>  467
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A*02:01-KLAEAIFKL

<400>  18

Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      


Ser Tyr Ile Tyr Ala Lys Leu Ala Glu Ala Ile Phe Lys Leu Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Asp Asp 
        35                  40                  45              


Asp Lys Gly Ile Gln Arg Thr Pro Lys Ile Gln Val Tyr Ser Arg His 
    50                  55                  60                  


Pro Ala Glu Asn Gly Lys Ser Asn Phe Leu Asn Cys Tyr Val Ser Gly 
65                  70                  75                  80  


Phe His Pro Ser Asp Ile Glu Val Asp Leu Leu Lys Asn Gly Glu Arg 
                85                  90                  95      


Ile Glu Lys Val Glu His Ser Asp Leu Ser Phe Ser Lys Asp Trp Ser 
            100                 105                 110         


Phe Tyr Leu Leu Tyr Tyr Thr Glu Phe Thr Pro Thr Glu Lys Asp Glu 
        115                 120                 125             


Tyr Ala Cys Arg Val Asn His Val Thr Leu Ser Gln Pro Lys Ile Val 
    130                 135                 140                 


Lys Trp Asp Arg Asp Met Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser His Ser Met Arg Tyr Phe 
                165                 170                 175     


Phe Thr Ser Val Ser Arg Pro Gly Arg Gly Glu Pro Arg Phe Ile Ala 
            180                 185                 190         


Val Gly Tyr Val Asp Asp Thr Gln Phe Val Arg Phe Asp Ser Asp Ala 
        195                 200                 205             


Ala Ser Gln Arg Met Glu Pro Arg Ala Pro Trp Ile Glu Gln Glu Gly 
    210                 215                 220                 


Pro Glu Tyr Trp Asp Gly Glu Thr Arg Lys Val Lys Ala His Ser Gln 
225                 230                 235                 240 


Thr His Arg Val Asp Leu Gly Thr Leu Arg Gly Tyr Tyr Asn Gln Ser 
                245                 250                 255     


Glu Ala Gly Ser His Thr Val Gln Arg Met Tyr Gly Cys Asp Val Gly 
            260                 265                 270         


Ser Asp Trp Arg Phe Leu Arg Gly Tyr His Gln Tyr Ala Tyr Asp Gly 
        275                 280                 285             


Lys Asp Tyr Ile Ala Leu Lys Glu Asp Leu Arg Ser Trp Thr Ala Ala 
    290                 295                 300                 


Asp Met Ala Ala Gln Thr Thr Lys His Lys Trp Glu Ala Ala His Val 
305                 310                 315                 320 


Ala Glu Gln Leu Arg Ala Tyr Leu Glu Gly Thr Cys Val Glu Trp Leu 
                325                 330                 335     


Arg Arg Tyr Leu Glu Asn Gly Lys Glu Thr Leu Gln Arg Thr Asp Ala 
            340                 345                 350         


Pro Lys Thr His Met Thr His His Ala Val Ser Asp His Glu Ala Thr 
        355                 360                 365             


Leu Arg Cys Trp Ala Leu Ser Phe Tyr Pro Ala Glu Ile Thr Leu Thr 
    370                 375                 380                 


Trp Gln Arg Asp Gly Glu Asp Gln Thr Gln Asp Thr Glu Leu Val Glu 
385                 390                 395                 400 


Thr Arg Pro Ala Gly Asp Gly Thr Phe Gln Lys Trp Ala Ala Val Val 
                405                 410                 415     


Val Pro Ser Gly Gln Glu Gln Arg Tyr Thr Cys His Val Gln His Glu 
            420                 425                 430         


Gly Leu Pro Lys Pro Leu Thr Leu Arg Trp Glu Pro Gly Ser Leu His 
        435                 440                 445             


His Ile Leu Asp Ala Gln Lys Met Val Trp Asn His Arg His His His 
    450                 455                 460                 


His His His 
465         


<210>  19
<211>  467
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A*01:01-YAETAAFAY

<400>  19

Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      


Ser Tyr Ile Tyr Ala Tyr Ala Glu Thr Ala Ala Phe Ala Tyr Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Asp Asp 
        35                  40                  45              


Asp Lys Gly Ile Gln Arg Thr Pro Lys Ile Gln Val Tyr Ser Arg His 
    50                  55                  60                  


Pro Ala Glu Asn Gly Lys Ser Asn Phe Leu Asn Cys Tyr Val Ser Gly 
65                  70                  75                  80  


Phe His Pro Ser Asp Ile Glu Val Asp Leu Leu Lys Asn Gly Glu Arg 
                85                  90                  95      


Ile Glu Lys Val Glu His Ser Asp Leu Ser Phe Ser Lys Asp Trp Ser 
            100                 105                 110         


Phe Tyr Leu Leu Tyr Tyr Thr Glu Phe Thr Pro Thr Glu Lys Asp Glu 
        115                 120                 125             


Tyr Ala Cys Arg Val Asn His Val Thr Leu Ser Gln Pro Lys Ile Val 
    130                 135                 140                 


Lys Trp Asp Arg Asp Met Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser His Ser Met Arg Tyr Phe 
                165                 170                 175     


Phe Thr Ser Val Ser Arg Pro Gly Arg Gly Glu Pro Arg Phe Ile Ala 
            180                 185                 190         


Val Gly Tyr Val Asp Asp Thr Gln Phe Val Arg Phe Asp Ser Asp Ala 
        195                 200                 205             


Ala Ser Gln Lys Met Glu Pro Arg Ala Pro Trp Ile Glu Gln Glu Gly 
    210                 215                 220                 


Pro Glu Tyr Trp Asp Gln Glu Thr Arg Asn Met Lys Ala His Ser Gln 
225                 230                 235                 240 


Thr Asp Arg Ala Asn Leu Gly Thr Leu Arg Gly Tyr Tyr Asn Gln Ser 
                245                 250                 255     


Glu Asp Gly Ser His Thr Ile Gln Ile Met Tyr Gly Cys Asp Val Gly 
            260                 265                 270         


Pro Asp Gly Arg Phe Leu Arg Gly Tyr Arg Gln Asp Ala Tyr Asp Gly 
        275                 280                 285             


Lys Asp Tyr Ile Ala Leu Asn Glu Asp Leu Arg Ser Trp Thr Ala Ala 
    290                 295                 300                 


Asp Met Ala Ala Gln Ile Thr Lys Arg Lys Trp Glu Ala Val His Ala 
305                 310                 315                 320 


Ala Glu Gln Arg Arg Val Tyr Leu Glu Gly Arg Cys Val Asp Gly Leu 
                325                 330                 335     


Arg Arg Tyr Leu Glu Asn Gly Lys Glu Thr Leu Gln Arg Thr Asp Pro 
            340                 345                 350         


Pro Lys Thr His Met Thr His His Pro Ile Ser Asp His Glu Ala Thr 
        355                 360                 365             


Leu Arg Cys Trp Ala Leu Gly Phe Tyr Pro Ala Glu Ile Thr Leu Thr 
    370                 375                 380                 


Trp Gln Arg Asp Gly Glu Asp Gln Thr Gln Asp Thr Glu Leu Val Glu 
385                 390                 395                 400 


Thr Arg Pro Ala Gly Asp Gly Thr Phe Gln Lys Trp Ala Ala Val Val 
                405                 410                 415     


Val Pro Ser Gly Glu Glu Gln Arg Tyr Thr Cys His Val Gln His Glu 
            420                 425                 430         


Gly Leu Pro Lys Pro Leu Thr Leu Arg Trp Glu Pro Gly Ser Leu His 
        435                 440                 445             


His Ile Leu Asp Ala Gln Lys Met Val Trp Asn His Arg His His His 
    450                 455                 460                 


His His His 
465         


<210>  20
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mellitin nucleotide sequence 1

<400>  20
atgaagtttc ttgtgaatgt agcgctcgtt ttcatggtgg tatatatctc atatatctat       60

gcg                                                                     63


<210>  21
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Enterokinase cleavable linker

<400>  21
ggtggaggtg gctctggagg cggcggctcc ggaggcggtg gtagtgatga cgatgacaaa       60

gga                                                                     63


<210>  22
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GS-Linker

<400>  22
ggaggaggcg gatcaggagg cggaggaagt ggtggtggag gttctggtgg tggcggtagt       60


<210>  23
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AVFAAASDAK

<400>  23
gctgtattcg ctgcggcttc cgatgccaaa                                        30


<210>  24
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AVFDRKSDAK

<400>  24
gctgtattcg ataggaagtc cgatgccaaa                                        30


<210>  25
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KILGRVFFV

<400>  25
aaaattttgg gtagggtttt ttttgtg                                           27


<210>  26
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KLAEAIFKL

<400>  26
aagctcgcag aggcgatttt caaactg                                           27


<210>  27
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YAETAAFAY

<400>  27
tatgcagaaa cggcggcatt tgcttat                                           27


<210>  28
<211>  297
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Beta-2 microglobulin

<400>  28
atccaaagaa ctccgaagat ccaagtgtac tctagacacc ctgctgaaaa tggtaaatcc       60

aacttcctca actgttacgt gagcggtttc cacccgtctg atatagaggt tgacctcctc      120

aagaatggcg aacgcatcga aaaggttgaa cactcagacc tctctttcag caaagattgg      180

agcttttatc ttttgtatta cactgagttt accccgacag agaaagacga atatgcatgc      240

agagtgaatc acgtgacgtt gtcgcagccg aaaatcgtga aatgggaccg cgatatg         297


<210>  29
<211>  819
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A*11:01 Heavy chain

<400>  29
cattcgatgc gttacttcta cacgtcggtg tctaggcccg gcagaggaga gccccgtttc       60

atagcggtag gttacgttga cgacacgcaa ttcgtgcgtt ttgactcgga cgccgcctca      120

caaagaatgg aaccccgcgc gccctggatt gagcaagagg gacctgaata ctgggatcaa      180

gaaacgagga acgtgaaggc gcagtcgcaa actgataggg tcgatctggg aacccttagg      240

ggatattata accaaagcga agacggatct catactattc aaattatgta tggttgcgac      300

gttggccccg atggccgctt tcttcgtgga tacaggcagg acgcgtacga cggaaaggat      360

tatattgcgt tgaatgaaga tcttaggtcg tggacagcgg cggacatggc cgctcaaatc      420

accaaacgta aatgggaagc cgcacacgcc gcagaacaac agcgcgctta tttggaaggc      480

agatgtgtag agtggttgcg tcgttatctc gaaaacggta aagaaacact ccaacgcacc      540

gatcccccga agactcatat gactcaccac ccaatatctg accatgaggc gacgttgaga      600

tgttgggcat tgggattcta tccggcagaa ataacgctca catggcagcg tgacggagag      660

gatcaaacgc aagatacgga acttgtggag acacgccctg cgggtgacgg aacctttcag      720

aagtgggcag cagttgtagt cccaagcggc gaggaacaac gctatacctg ccacgtacaa      780

cacgaaggac tccctaagcc actcactctc cgctgggaa                             819


<210>  30
<211>  819
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A*02:01 Heavy chain

<400>  30
catagtatga ggtatttctt tacgagtgtg tcgcgccccg gcagaggcga acctcgcttt       60

atagctgtag gatatgtcga tgacacacaa ttcgtacgct ttgattccga tgcagcgtct      120

caaagaatgg agcctagagc gccgtggata gagcaagaag gccctgaata ttgggatggc      180

gagactcgca aagtaaaggc tcacagtcaa actcatcgtg tagatctggg cacgttgcgt      240

ggctattaca atcaatctga agcaggatcg cacactgtac agcgtatgta cggctgcgat      300

gttggcagcg actggcgctt tcttagaggt taccatcaat atgcgtatga cggcaaggac      360

tacattgcat tgaaggagga cttgaggagt tggaccgcag cggatatggc cgcgcagacc      420

acaaagcata aatgggaggc cgctcatgtc gctgaacagc ttagagctta tttggaaggc      480

acctgcgtag aatggctgcg ccgttacttg gagaacggca aggaaacctt gcagaggacc      540

gatgcgccta aaacacatat gacacaccac gccgtatccg atcacgaagc taccctgagg      600

tgttgggctc tttcctttta ccctgccgag attacattga catggcagcg tgacggcgaa      660

gaccaaacac aagacactga gttggtggag acccgcccgg ctggcgacgg aaccttccaa      720

aagtgggccg ctgtagtagt cccatccgga caggaacaac gctatacgtg tcacgttcaa      780

catgaaggtc tgcctaagcc gctcaccttg cgctgggaa                             819


<210>  31
<211>  819
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A*01:01 Heavy chain

<400>  31
cattcaatgc gttacttctt tacgtccgta tccaggccag gcaggggaga gccgagattc       60

atagctgtcg gctacgtcga tgacacgcag ttcgttcgtt tcgatagcga cgccgcgtcc      120

cagaaaatgg aacccagggc tccgtggatc gagcaggagg gaccggaata ctgggatcaa      180

gagacacgta atatgaaggc acacagtcaa acagatcgtg caaacctcgg aacgctgaga      240

ggttattata atcagtccga agacggttcc cacaccatac agattatgta cggatgtgac      300

gtgggccctg atggcagatt cctcagaggc taccgccagg acgcctatga tggcaaagat      360

tatattgcct tgaatgagga tctgcgttca tggacggcgg ctgatatggc cgcgcagatc      420

acaaagagaa aatgggaagc tgttcatgcg gcggaacagc gccgtgttta cttggagggt      480

aggtgcgtcg acggactgag acgttatttg gagaatggca aggaaacgct tcaaagaacg      540

gaccctccca agacacacat gactcatcat cccatatctg atcatgaggc aactcttagg      600

tgttgggctc ttggcttcta ccctgctgag attactctta catggcaaag ggacggcgaa      660

gatcagaccc aagacactga gttggttgag accagacctg cgggtgacgg tacatttcag      720

aaatgggcgg cagtcgtggt gccgagcggt gaggaacaac gttacacatg tcatgttcag      780

catgaaggtc tgcctaagcc ccttacactg cgctgggaa                             819


<210>  32
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BirA recognition site

<400>  32
ctgcaccaca tcttggacgc acaaaagatg gtctggaatc accgt                       45


<210>  33
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  His6X

<400>  33
catcaccatc atcaccac                                                     18


<210>  34
<211>  1419
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A*11:01-AVFDRKSDAK nucleotide

<400>  34
ggatccatga agtttcttgt gaatgtagcg ctcgttttca tggtggtata tatctcatat       60

atctatgcgg ctgtattcga taggaagtcc gatgccaaag gtggaggtgg ctctggaggc      120

ggcggctccg gaggcggtgg tagtgatgac gatgacaaag gaatccaaag aactccgaag      180

atccaagtgt actctagaca ccctgctgaa aatggtaaat ccaacttcct caactgttac      240

gtgagcggtt tccacccgtc tgatatagag gttgacctcc tcaagaatgg cgaacgcatc      300

gaaaaggttg aacactcaga cctctctttc agcaaagatt ggagctttta tcttttgtat      360

tacactgagt ttaccccgac agagaaagac gaatatgcat gcagagtgaa tcacgtgacg      420

ttgtcgcagc cgaaaatcgt gaaatgggac cgcgatatgg gaggaggcgg atcaggaggc      480

ggaggaagtg gtggtggagg ttctggtggt ggcggtagtc attcgatgcg ttacttctac      540

acgtcggtgt ctaggcccgg cagaggagag ccccgtttca tagcggtagg ttacgttgac      600

gacacgcaat tcgtgcgttt tgactcggac gccgcctcac aaagaatgga accccgcgcg      660

ccctggattg agcaagaggg acctgaatac tgggatcaag aaacgaggaa cgtgaaggcg      720

cagtcgcaaa ctgatagggt cgatctggga acccttaggg gatattataa ccaaagcgaa      780

gacggatctc atactattca aattatgtat ggttgcgacg ttggccccga tggccgcttt      840

cttcgtggat acaggcagga cgcgtacgac ggaaaggatt atattgcgtt gaatgaagat      900

cttaggtcgt ggacagcggc ggacatggcc gctcaaatca ccaaacgtaa atgggaagcc      960

gcacacgccg cagaacaaca gcgcgcttat ttggaaggca gatgtgtaga gtggttgcgt     1020

cgttatctcg aaaacggtaa agaaacactc caacgcaccg atcccccgaa gactcatatg     1080

actcaccacc caatatctga ccatgaggcg acgttgagat gttgggcatt gggattctat     1140

ccggcagaaa taacgctcac atggcagcgt gacggagagg atcaaacgca agatacggaa     1200

cttgtggaga cacgccctgc gggtgacgga acctttcaga agtgggcagc agttgtagtc     1260

ccaagcggcg aggaacaacg ctatacctgc cacgtacaac acgaaggact ccctaagcca     1320

ctcactctcc gctgggaacc gggtagtctg caccacatct tggacgcaca aaagatggtc     1380

tggaatcacc gtcatcacca tcatcaccac tagctgcag                            1419


<210>  35
<211>  1419
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A*11:01-AVFAAASDAK nucleotide

<400>  35
ggatccatga agtttcttgt gaatgtagcg ctcgttttca tggtggtata tatctcatat       60

atctatgcgg ctgtattcgc tgcggcttcc gatgccaaag gtggaggtgg ctctggaggc      120

ggcggctccg gaggcggtgg tagtgatgac gatgacaaag gaatccaaag aactccgaag      180

atccaagtgt actctagaca ccctgctgaa aatggtaaat ccaacttcct caactgttac      240

gtgagcggtt tccacccgtc tgatatagag gttgacctcc tcaagaatgg cgaacgcatc      300

gaaaaggttg aacactcaga cctctctttc agcaaagatt ggagctttta tcttttgtat      360

tacactgagt ttaccccgac agagaaagac gaatatgcat gcagagtgaa tcacgtgacg      420

ttgtcgcagc cgaaaatcgt gaaatgggac cgcgatatgg gaggaggcgg atcaggaggc      480

ggaggaagtg gtggtggagg ttctggtggt ggcggtagtc attcgatgcg ttacttctac      540

acgtcggtgt ctaggcccgg cagaggagag ccccgtttca tagcggtagg ttacgttgac      600

gacacgcaat tcgtgcgttt tgactcggac gccgcctcac aaagaatgga accccgcgcg      660

ccctggattg agcaagaggg acctgaatac tgggatcaag aaacgaggaa cgtgaaggcg      720

cagtcgcaaa ctgatagggt cgatctggga acccttaggg gatattataa ccaaagcgaa      780

gacggatctc atactattca aattatgtat ggttgcgacg ttggccccga tggccgcttt      840

cttcgtggat acaggcagga cgcgtacgac ggaaaggatt atattgcgtt gaatgaagat      900

cttaggtcgt ggacagcggc ggacatggcc gctcaaatca ccaaacgtaa atgggaagcc      960

gcacacgccg cagaacaaca gcgcgcttat ttggaaggca gatgtgtaga gtggttgcgt     1020

cgttatctcg aaaacggtaa agaaacactc caacgcaccg atcccccgaa gactcatatg     1080

actcaccacc caatatctga ccatgaggcg acgttgagat gttgggcatt gggattctat     1140

ccggcagaaa taacgctcac atggcagcgt gacggagagg atcaaacgca agatacggaa     1200

cttgtggaga cacgccctgc gggtgacgga acctttcaga agtgggcagc agttgtagtc     1260

ccaagcggcg aggaacaacg ctatacctgc cacgtacaac acgaaggact ccctaagcca     1320

ctcactctcc gctgggaacc gggtagtctg caccacatct tggacgcaca aaagatggtc     1380

tggaatcacc gtcatcacca tcatcaccac tagctgcag                            1419


<210>  36
<211>  1416
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A*02:01-KILGRVFFV nucleotide

<400>  36
ggatccatga aatttctcgt taatgttgca cttgtcttca tggtggtcta tataagctac       60

atatacgcga aaattttggg tagggttttt tttgtgggcg gcggtggatc tggcggaggc      120

ggttcaggcg gaggcggtag tgatgacgat gataagggca tacagcgtac tcctaagatc      180

caggtttact ctagacatcc ggctgagaac ggcaaatcaa actttcttaa ttgctacgtc      240

agcggctttc atccatcaga tatagaggtc gacctgctga agaatggcga acgcattgaa      300

aaagtcgaac atagtgacct ctcattcagc aaagactggt ccttttatct cctctactat      360

acggagttta cacctactga aaaggacgag tacgcctgta gagtaaatca tgtgacgctc      420

agccagccga agatagtgaa atgggatcgt gacatgggag gcggaggctc tggaggcggt      480

ggctccggtg gtggtggtag cggaggtgga ggaagccata gtatgaggta tttctttacg      540

agtgtgtcgc gccccggcag aggcgaacct cgctttatag ctgtaggata tgtcgatgac      600

acacaattcg tacgctttga ttccgatgca gcgtctcaaa gaatggagcc tagagcgccg      660

tggatagagc aagaaggccc tgaatattgg gatggcgaga ctcgcaaagt aaaggctcac      720

agtcaaactc atcgtgtaga tctgggcacg ttgcgtggct attacaatca atctgaagca      780

ggatcgcaca ctgtacagcg tatgtacggc tgcgatgttg gcagcgactg gcgctttctt      840

agaggttacc atcaatatgc gtatgacggc aaggactaca ttgcattgaa ggaggacttg      900

aggagttgga ccgcagcgga tatggccgcg cagaccacaa agcataaatg ggaggccgct      960

catgtcgctg aacagcttag agcttatttg gaaggcacct gcgtagaatg gctgcgccgt     1020

tacttggaga acggcaagga aaccttgcag aggaccgatg cgcctaaaac acatatgaca     1080

caccacgccg tatccgatca cgaagctacc ctgaggtgtt gggctctttc cttttaccct     1140

gccgagatta cattgacatg gcagcgtgac ggcgaagacc aaacacaaga cactgagttg     1200

gtggagaccc gcccggctgg cgacggaacc ttccaaaagt gggccgctgt agtagtccca     1260

tccggacagg aacaacgcta tacgtgtcac gttcaacatg aaggtctgcc taagccgctc     1320

accttgcgct gggaaccagg ctcattgcat catattcttg atgcacagaa aatggtatgg     1380

aatcatcgcc atcatcatca tcatcactag ctgcag                               1416


<210>  37
<211>  1416
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A*02:01-KLAEAIFKL nucleotide

<400>  37
ggatccatga agtttttggt gaacgtcgcg ctcgtcttca tggtcgttta catatcatac       60

atttatgcga agctcgcaga ggcgattttc aaactgggag gaggtggttc gggcggaggc      120

ggcagtggag gtggtggctc agatgacgat gacaagggta ttcaaaggac accgaaaatc      180

caagtgtatt ctagacatcc ggctgagaac ggcaaatcaa actttcttaa ttgctacgtc      240

agcggctttc atccatcaga tatagaggtc gacctgctga agaatggcga acgcattgaa      300

aaagtcgaac atagtgacct ctcattcagc aaagactggt ccttttatct cctctactat      360

acggagttta cacctactga aaaggacgag tacgcctgta gagtaaatca tgtgacgctc      420

agccagccga agatagtgaa atgggatcgt gacatgggag gcggaggctc tggaggcggt      480

ggctccggtg gtggtggtag cggaggtgga ggaagccata gtatgaggta tttctttacg      540

agtgtgtcgc gccccggcag aggcgaacct cgctttatag ctgtaggata tgtcgatgac      600

acacaattcg tacgctttga ttccgatgca gcgtctcaaa gaatggagcc tagagcgccg      660

tggatagagc aagaaggccc tgaatattgg gatggcgaga ctcgcaaagt aaaggctcac      720

agtcaaactc atcgtgtaga tctgggcacg ttgcgtggct attacaatca atctgaagca      780

ggatcgcaca ctgtacagcg tatgtacggc tgcgatgttg gcagcgactg gcgctttctt      840

agaggttacc atcaatatgc gtatgacggc aaggactaca ttgcattgaa ggaggacttg      900

aggagttgga ccgcagcgga tatggccgcg cagaccacaa agcataaatg ggaggccgct      960

catgtcgctg aacagcttag agcttatttg gaaggcacct gcgtagaatg gctgcgccgt     1020

tacttggaga acggcaagga aaccttgcag aggaccgatg cgcctaaaac acatatgaca     1080

caccacgccg tatccgatca cgaagctacc ctgaggtgtt gggctctttc cttttaccct     1140

gccgagatta cattgacatg gcagcgtgac ggcgaagacc aaacacaaga cactgagttg     1200

gtggagaccc gcccggctgg cgacggaacc ttccaaaagt gggccgctgt agtagtccca     1260

tccggacagg aacaacgcta tacgtgtcac gttcaacatg aaggtctgcc taagccgctc     1320

accttgcgct gggaaccagg ctcattgcat catattcttg atgcacagaa aatggtatgg     1380

aatcatcgcc atcatcatca tcatcactag ctgcag                               1416


<210>  38
<211>  1416
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A*01:01- YAETAAFAY nucleotide

<400>  38
ggatccatga aatttctcgt taatgttgca cttgtcttca tggtggtcta tataagctac       60

atatacgcgt atgcagaaac ggcggcattt gcttatggcg gcggtggatc tggcggaggc      120

ggttcaggcg gaggcggtag tgatgacgat gataagggca tacagcgtac tcctaagatc      180

caggtttact ctagacatcc ggctgagaac ggcaaatcaa actttcttaa ttgctacgtc      240

agcggctttc atccatcaga tatagaggtc gacctgctga agaatggcga acgcattgaa      300

aaagtcgaac atagtgacct ctcattcagc aaagactggt ccttttatct cctctactat      360

acggagttta cacctactga aaaggacgag tacgcctgta gagtaaatca tgtgacgctc      420

agccagccga agatagtgaa atgggatcgt gacatgggag gcggaggctc tggaggcggt      480

ggctccggtg gtggtggtag cggaggtgga ggaagccatt caatgcgtta cttctttacg      540

tccgtatcca ggccaggcag gggagagccg agattcatag ctgtcggcta cgtcgatgac      600

acgcagttcg ttcgtttcga tagcgacgcc gcgtcccaga aaatggaacc cagggctccg      660

tggatcgagc aggagggacc ggaatactgg gatcaagaga cacgtaatat gaaggcacac      720

agtcaaacag atcgtgcaaa cctcggaacg ctgagaggtt attataatca gtccgaagac      780

ggttcccaca ccatacagat tatgtacgga tgtgacgtgg gccctgatgg cagattcctc      840

agaggctacc gccaggacgc ctatgatggc aaagattata ttgccttgaa tgaggatctg      900

cgttcatgga cggcggctga tatggccgcg cagatcacaa agagaaaatg ggaagctgtt      960

catgcggcgg aacagcgccg tgtttacttg gagggtaggt gcgtcgacgg actgagacgt     1020

tatttggaga atggcaagga aacgcttcaa agaacggacc ctcccaagac acacatgact     1080

catcatccca tatctgatca tgaggcaact cttaggtgtt gggctcttgg cttctaccct     1140

gctgagatta ctcttacatg gcaaagggac ggcgaagatc agacccaaga cactgagttg     1200

gttgagacca gacctgcggg tgacggtaca tttcagaaat gggcggcagt cgtggtgccg     1260

agcggtgagg aacaacgtta cacatgtcat gttcagcatg aaggtctgcc taagcccctt     1320

acactgcgct gggaaccggg tagtctgcac cacatcttgg acgcacaaaa gatggtctgg     1380

aatcaccgtc atcaccatca tcaccactag ctgcag                               1416


<210>  39
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EBV peptide 1

<400>  39

Ile Val Thr Asp Phe Ser Val Ile Lys 
1               5                   


<210>  40
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EBV peptide 2

<400>  40

Asn Thr Leu Glu Gln Thr Val Lys Lys 
1               5                   


<210>  41
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CMV peptide

<400>  41

Asn Leu Val Pro Met Val Ala Thr Val 
1               5                   


<210>  42
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CMV-derived peptide

<400>  42

Val Glu Thr Glu His Asp Thr Leu Leu Tyr 
1               5                   10  


<210>  43
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavable DDDDK peptide

<400>  43

Asp Asp Asp Asp Lys 
1               5   


<210>  44
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GTSGSPIVNR

<400>  44

Gly Thr Ser Gly Ser Pro Ile Val Asn Arg 
1               5                   10  


<210>  45
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ATYGWNLVK

<400>  45

Ala Thr Tyr Gly Trp Asn Leu Val Lys 
1               5                   


<210>  46
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SIIPSGPLK

<400>  46

Ser Ile Ile Pro Ser Gly Pro Leu Lys 
1               5                   


<210>  47
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RMVLASTTAK

<400>  47

Arg Met Val Leu Ala Ser Thr Thr Ala Lys 
1               5                   10  


<210>  48
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KSMREEYRK

<400>  48

Lys Ser Met Arg Glu Glu Tyr Arg Lys 
1               5                   


<210>  49
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ATIGTAMYK

<400>  49

Ala Thr Ile Gly Thr Ala Met Tyr Lys 
1               5                   


<210>  50
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SSCSSCPLSK

<400>  50

Ser Ser Cys Ser Ser Cys Pro Leu Ser Lys 
1               5                   10  


<210>  51
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  YVNVNMGLK

<400>  51

Tyr Val Asn Val Asn Met Gly Leu Lys 
1               5                   


<210>  52
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLDKGTYTL

<400>  52

Phe Leu Asp Lys Gly Thr Tyr Thr Leu 
1               5                   


<210>  53
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VLEETSVML

<400>  53

Val Leu Glu Glu Thr Ser Val Met Leu 
1               5                   


<210>  54
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLYALALLL

<400>  54

Phe Leu Tyr Ala Leu Ala Leu Leu Leu 
1               5                   


