                         SEQUENCE LISTING

<110>  Auckland Uniservices Limited
 
<120>  PEPTIDE CONJUGATE AMYLIN AGONISTS AND USES THEREOF

<130>  826187

<150>  US62/845145
<151>  2019-05-08

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  37
<212>  PRT
<213>  Homo sapiens

<400>  1

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Asn Phe Gly Ala Ile Leu Ser Ser Thr Asn Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  2
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide sequence

<400>  2

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Asn Phe Gly Pro Ile Leu Pro Pro Thr Asn Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  3
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide sequence


<220>
<221>  MISC_FEATURE
<223>  the N-terminal is optionally acetylated

<220>
<221>  MISC_FEATURE
<223>  one or more of Xaa1, Xaa3-Xaa5, Xaa10, Xaa11, Xaa14-Xaa18, 
       Xaa20-Xaa24, Xaa26, Xaa27, Xaa31, Xaa32, and Xaa34 to Xaa36 is 
       cysteine or homocysteine and is covently conjugated to a 
       lipid-containing moiety

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa1 is deleted or independently selected from Ala, Ile, Val, 
       Leu, Pro, Phe, Met, Typ, Lys, Arg, Gln, Asn, Ser, Glu, Gly, Cys, 
       Hcy and His

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa3 is independently selected from Gly, Pro, His, Arg, Ser, Asn,
       Gln, Cys, Hcy and Lys

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa4 is independently selected from Met; Leu, Phe, Ile, Thr, Cys,
       Hcy and Ser

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa5 is independently selected from Ser, Thr, Ala, Val, Leu, Cys,
       Hcy and Ile

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa10 is independently selected from Ala, Val, Leu, Ile, Gln, 
       Cys, Hcy and Asn

<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  Xaa11 is independently selected from His, Asn, Gln, Lys, Cys, Hcy
       and Arg

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa14 is independently selected from Ala, Val, Leu, Ile, Glu, 
       Asn, Gln, Lys, His, Cys, Hcy and Arg

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  Xaa15 is independently selected from Ala, Met, Leu, Tyr, Trp, 
       Cys, Hcy and Phe

<220>
<221>  MISC_FEATURE
<222>  (16)..(16)
<223>  Xaa16 is independently selected from Ala, Ile, Val, Met, Phe, 
       Cys, Hcy and Leu

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa17 is independently selected from Ala, Asp, Val, Leu, Ile, 
       His, Arg, Lys, Val, Met, Cys, Hcy and Phe

<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  Xaa18 is independently selected from Arg, Pro, Lys, Gln, Asn, 
       Cys, Hcy and His

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  Xaa20 is independently selected from Arg, Lys, Gln, Asn, Ser, 
       Cys, Hcy and Thr

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa21 is independently selected from Ala, Lys, Gln, Ser, Asn, 
       His, Cys, Hcy and Arg

<220>
<221>  MISC_FEATURE
<222>  (22)..(22)
<223>  Xaa22 is independently selected from Lys, Arg, Glu, Gln, Ser, 
       Thr, Asn, His, Cys, Hcy and Arg

<220>
<221>  MISC_FEATURE
<222>  (23)..(23)
<223>  Xaa23 is independently selected from Leu, Ile, Val, Met, Ala, 
       Cys, Hcy and Phe

<220>
<221>  MISC_FEATURE
<222>  (24)..(24)
<223>  Xaa24 is independently selected from Ala, Gly, Cys, Hcy and Pro

<220>
<221>  MISC_FEATURE
<222>  (26)..(26)
<223>  Xaa26 is independently selected from Ala, Val, Ile, Leu, Met, 
       Phe, Pro, Arg, Cys, Hcy and Ile

<220>
<221>  MISC_FEATURE
<222>  (27)..(27)
<223>  Xaa27 is independently selected from Phe, Val, Ile, Ala, Met, 
       Cys, Hcy and Leu

<220>
<221>  MISC_FEATURE
<222>  (31)..(31)
<223>  Xaa31 is independently selected from Lys, Arg, Gln, Ser, Glu, 
       Asp, Asn, Cys, Hcy and His

<220>
<221>  MISC_FEATURE
<222>  (32)..(32)
<223>  Xaa32 is independently selected from Met, Leu, Phe, Ile, Ala, 
       Cys, Hcy and Val

<220>
<221>  MISC_FEATURE
<222>  (34)..(34)
<223>  Xaa34 is independently selected from Ala, Val, Leu, Ile, Cys, 
       Hcy, Thr, Tyr, Asp, Gln and Ser

<220>
<221>  MISC_FEATURE
<222>  (35)..(35)
<223>  Xaa35 is independently selected from His, Arg, Lys, Asp, Gln, 
       Glu, Cys, Hcy and Asn

<220>
<221>  MISC_FEATURE
<222>  (36)..(36)
<223>  Xaa36 is independently selected from Ala, Cys, Hcy, Ser, Tyr, 
       Asp, Gln and Thr

<220>
<221>  MISC_FEATURE
<222>  (37)..(37)
<223>  Xaa37 is independently selected from Pro and Tyr

<400>  3

Xaa Cys Xaa Xaa Xaa Thr Cys Ala Thr Xaa Xaa Leu Ala Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Ser Xaa Xaa Xaa Xaa Xaa Pro Xaa Xaa Pro Pro Thr Xaa Xaa 
            20                  25                  30          


Gly Xaa Xaa Xaa Xaa 
        35          


<210>  4
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide sequence


<220>
<221>  MISC_FEATURE
<223>  the C-terminal amino acid is amidated

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  X is a cysteine or homocysteine and wherein X in the peptide is 
       covalently conjugated to a lipid containing moiety

<400>  4

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Xaa Asn Phe Gly Pro Ile Leu Pro Pro Thr Asn Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  5
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide sequence


<220>
<221>  MISC_FEATURE
<223>  the C-terminal amino acid is amidated

<220>
<221>  MISC_FEATURE
<222>  (35)..(35)
<223>  X is a cysteine or homocysteine and wherein X in the peptide is 
       covalently conjugated to a lipid containing moiety

<400>  5

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Asn Phe Gly Pro Ile Leu Pro Pro Thr Asn Val 
            20                  25                  30          


Gly Ser Xaa Thr Tyr 
        35          


<210>  6
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide sequence


<220>
<221>  MISC_FEATURE
<223>  the N-terminal amino acid is acetylated

<220>
<221>  MISC_FEATURE
<223>  the C-terminal amino acid is amidated

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  X is a cysteine or homocysteine and wherein X in the peptide is 
       covalently conjugated to a lipid containing moiety

<400>  6

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Xaa Asn Phe Gly Pro Ile Leu Pro Pro Thr Asn Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  7
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide sequence


<220>
<221>  MISC_FEATURE
<223>  the C-terminal amino acid is amidated

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  X is a cysteine or homocysteine and wherein X in the peptide is 
       covalently conjugated to a lipid containing moiety

<400>  7

Xaa Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Asn Phe Gly Pro Ile Leu Pro Pro Thr Asn Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  8
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide sequence


<220>
<221>  MISC_FEATURE
<223>  the C-terminal amino acid is amidated

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  X is a cysteine or homocysteine and wherein X in the peptide is 
       covalently conjugated to a lipid containing moiety

<400>  8

Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu Val 
1               5                   10                  15      


His Ser Ser Xaa Asn Phe Gly Pro Ile Leu Pro Pro Thr Asn Val Gly 
            20                  25                  30          


Ser Asn Thr Tyr 
        35      


<210>  9
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide sequence


<220>
<221>  MISC_FEATURE
<223>  the N-terminal amino acid is acetylated

<220>
<221>  MISC_FEATURE
<223>  the C-terminal amino acid is amidated

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  X is a cysteine or homocysteine and wherein X in the peptide is 
       covalently conjugated to a lipid containing moiety

<400>  9

Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu Val 
1               5                   10                  15      


His Ser Ser Xaa Asn Phe Gly Pro Ile Leu Pro Pro Thr Asn Val Gly 
            20                  25                  30          


Ser Asn Thr Tyr 
        35      


<210>  10
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide sequence


<220>
<221>  MISC_FEATURE
<223>  the C-terminal amino acid is amidated

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  X is a cysteine or homocysteine and wherein X in the peptide is 
       covalently conjugated to a lipid containing moiety

<400>  10

Lys Cys Asn Thr Ala Thr Cys Ala Thr Ala Arg Leu Ala Ala Phe Leu 
1               5                   10                  15      


Ala His Ser Ser Xaa Asn Phe Gly Pro Ile Leu Pro Pro Thr Asn Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  11
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide sequence


<220>
<221>  MISC_FEATURE
<223>  the N-terminal amino acid is acetylated

<220>
<221>  MISC_FEATURE
<223>  the C-terminal amino acid is amidated

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  X is a cysteine or homocysteine and wherein X in the peptide is 
       covalently conjugated to a lipid containing moiety

<400>  11

Lys Cys Asn Thr Ala Thr Cys Ala Thr Ala Arg Leu Ala Ala Phe Leu 
1               5                   10                  15      


Ala His Ser Ser Xaa Asn Phe Gly Pro Ile Leu Pro Pro Thr Asn Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  12
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide sequence


<220>
<221>  MISC_FEATURE
<223>  the C-terminal amino acid is amidated

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  X is a cysteine or homocysteine and wherein X in the peptide is 
       covalently conjugated to a lipid containing moiety

<400>  12

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Xaa Asn Ala Gly Pro Ile Leu Pro Pro Thr Asn Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  13
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide sequence


<220>
<221>  MISC_FEATURE
<223>  the C-terminal amino acid is amidated

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  X is a cysteine or homocysteine and wherein X in the peptide is 
       covalently conjugated to a lipid containing moiety

<400>  13

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Xaa Asn Phe Ala Pro Ile Leu Pro Pro Thr Asn Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  14
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide sequence


<220>
<221>  MISC_FEATURE
<223>  the C-terminal amino acid is amidated

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa1 is cysteine or homocysteine and is covently conjugated to a 
       lipid-containing moiety

<400>  14

Xaa Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Asn Phe Gly Pro Ile Leu Pro Pro Thr Asn Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


