                         SEQUENCE LISTING

<110>  DNAE DIAGNOSTICS LTD
 
<120>  Combined solution phase and solid phase DNA amplification

<130>  P9527WO1

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  18
<212>  DNA
<213>  Ser.marcescens_23s_F
<220>
<223>

<400>  1
agcccagaac ctgaatcg                                                     18


<210>  2
<211>  20
<212>  DNA
<213>  Ser.marcescens_23s_R
<220>
<223>

<400>  2
ccctactcat cgaactcacg                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  NH2_iSp18_SmR
<220>
<223> The C at position 1 is attached to Spacer 18, which is an 18-atom hexa-ethyleneglycol spacer (iSp18), which is attached to an amino modifier with six carbons (5AmMC6).

<400>  3
ccctactcat cgaactcacg                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  NH2_iSp18_SmIntRof
<220>
<223> The T at position 1 is attached to Spacer 18, which is an 18-atom hexa-ethyleneglycol spacer (iSp18), which is attached to an amino modifier with six carbons (5AmMC6).

<400>  4
tgtgtgttag tggaagcgtc                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  NH2_iSp18_EfR
<220>
<223> The G at position 1 is attached to Spacer 18, which is an 18-atom hexa-ethyleneglycol spacer (iSp18), which is attached to an amino modifier with six carbons (5AmMC6).

<400>  5
gggaggttca gttactaacg                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  NH2_iSp18_EfIntRof
<220>
<223> The G at position 1 is attached to Spacer 18, which is an 18-atom hexa-ethyleneglycol spacer (iSp18), which is attached to an amino modifier with six carbons (5AmMC6).

<400>  6
gatcgtaaaa ctctgttgtt                                                   20


<210>  7
<211>  99
<212>  DNA
<213>  NH2_ddl_5'+T15
<220>
<223> The T at position 1 is attached to an amino modifier with six carbons (5AmMC6).

<400>  7
tttttttttt tttttcgcgc ttcaattcct tgttcaacga ttgctcgaga atcatactga       60

taggctgttg ctaaagcatt ttgcagctct tctcggttt                              99


<210>  8
<211>  20
<212>  DNA
<213>  SM_IntRof_Cy3
<220>
<223> The T at position 1 is attached to a fluorescent dye (5 Cy3).

<400>  8
tgtgtgttag tggaagcgtc                                                   20


<210>  9
<211>  24
<212>  DNA
<213>  Spom_gp_1_608_23_PAr
<220>
<223>

<400>  9
ttcagtgagt tctcctgttg attg                                              24


<210>  10
<211>  23
<212>  DNA
<213>  Spom_gp_1_270_24_IDf
<220>
<223>

<400>  10
aacgttggta taggagaatg aag                                               23


<210>  11
<211>  62
<212>  DNA
<213>  T10C10_iSp18_Spo_gp_1_338+19_5'_Rof
<220>
<223> In between the C at position 20 and the A at position 21 is Spacer 18, which is an 18-atom hexa-ethyleneglycol spacer (iSp18).

<400>  11
tttttttttt cccccccccc aatactatta atatcacctc agtttcagct gggactaatg       60

aa                                                                      62


<210>  12
<211>  63
<212>  DNA
<213>  T10C10_iSp18_Spo_gp_1_369+24_5'_Rof
<220>
<223> In between the C at position 20 and the G at position 21 is Spacer 18, which is an 18-atom hexa-ethyleneglycol spacer (iSp18).

<400>  12
tttttttttt cccccccccc gggactaatg aattgttttt acctacttta tcccatgctc       60

acg                                                                     63


<210>  13
<211>  58
<212>  DNA
<213>  T10C10_iSp18_Spo_gp_1_436+15_5'_Rof
<220>
<223> In between the C at position 20 and the C at position 21 is Spacer 18, which is an 18-atom hexa-ethyleneglycol spacer (iSp18).

<400>  13
tttttttttt cccccccccc ccatacacag acataagata taacggcttc ccagaacc         58


<210>  14
<211>  61
<212>  DNA
<213>  T10C10_iSp18_Spo_gp_1_495+ 20_5'_Rof
<220>
<223> In between the C at position 20 and the G at position 21 is Spacer 18, which is an 18-atom hexa-ethyleneglycol spacer (iSp18).

<400>  14
tttttttttt cccccccccc gagcatatta tcctttaata ccattcgttt aatcccgtcc       60

a                                                                       61


<210>  15
<211>  62
<212>  DNA
<213>  T10C10_iSp18_Sa_nuc_1_186+19_Rof
<220>
<223> In between the C at position 20 and the A at position 21 is Spacer 18, which is an 18-atom hexa-ethyleneglycol spacer (iSp18).

<400>  15
tttttttttt cccccccccc agtctaagta gctcagcaaa tgcatcacaa acagataacg       60

gc                                                                      62


<210>  16
<211>  61
<212>  DNA
<213>  T10C10_iSp18_Sa_nuc_1_220+18_Rof
<220>
<223> In between the C at position 20 and the G at position 21 is Spacer 18, which is an 18-atom hexa-ethyleneglycol spacer (iSp18).

<400>  16
tttttttttt cccccccccc gataacggcg taaatagaag tggttctgaa gatccaacag       60

t                                                                       61


<210>  17
<211>  63
<212>  DNA
<213>  T10C10_iSp18_Sa_nuc_1_369+20_Rof
<220>
<223> In between the C at position 20 and the T at position 21 is Spacer 18, which is an 18-atom hexa-ethyleneglycol spacer (iSp18).

<400>  17
tttttttttt cccccccccc ttagactatt attggttgat acacctgaaa caaagcatcc       60

taa                                                                     63


<210>  18
<211>  62
<212>  DNA
<213>  T10C10_iSp18_Sa_nuc_1_415+19_Rof
<220>
<223> In between the C at position 20 and the A at position 21 is Spacer 18, which is an 18-atom hexa-ethyleneglycol spacer (iSp18).

<400>  18
tttttttttt cccccccccc aggtgtagag aaatatggtc ctgaagcaag tgcatttacg       60

aa                                                                      62


<210>  19
<211>  99
<212>  DNA
<213>  T10C10_ddl_5'-5
<220>
<223>

<400>  19
tttttttttt cccccccccc ttcaattcct tgttcaacga ttgctcgaga atcatactga       60

taggctgttg ctaaagcatt ttgcagctct tctcggttt                              99


<210>  20
<211>  23
<212>  DNA
<213>  Spo_gp_1_578_23_Cy3P_RC
<220>
<223> The C at position 1 is attached to a fluorescent dye (5 Cy3).

<400>  20
ctgcgcgttt acctactttg act                                               23


