                         SEQUENCE LISTING

<110>  INSERM
       CNRS
       Universit de Bordeaux
 
<120>  Lipid oligonucleotide antisense against antibiotic resistance

<130>  BET 20P1464

<150>  EP19305585.2
<151>  2019-05-09

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the LASOalpha antisense oligonucleotide

<400>  1
gcgcagtgat tttttaacca tggga                                             25


<210>  2
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the LASObeta antisense oligonucleotide

<400>  2
cgtgtaggta cggcagatc                                                    19


<210>  3
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the LASOgamma antisense oligonucleotide

<400>  3
tgaactggcg cagtgatttt ttaac                                             25


<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the LASOdelta antisense oligonucleotide

<400>  4
gtcggctcgg tacggtcgag a                                                 21


<210>  5
<211>  876
<212>  DNA
<213>  Escherichia coli

<400>  5
atggttaaaa aatcactgcg ccagttcacg ctgatggcga cggcaaccgt cacgctgttg       60

ttaggaagtg tgccgctgta tgcgcaaacg gcggacgtac agcaaaaact tgccgaatta      120

gagcggcagt cgggaggcag actgggtgtg gcattgatta acacagcaga taattcgcaa      180

atactttatc gtgctgatga gcgctttgcg atgtgcagca ccagtaaagt gatggccgcg      240

gccgcggtgc tgaagaaaag tgaaagcgaa ccgaatctgt taaatcagcg agttgagatc      300

aaaaaatctg accttgttaa ctataatccg attgcggaaa agcacgtcaa tgggacgatg      360

tcactggctg agcttagcgc ggccgcgcta cagtacagcg ataacgtggc gatgaataag      420

ctgattgctc acgttggcgg cccggctagc gtcaccgcgt tcgcccgaca gctgggagac      480

gaaacgttcc gtctcgaccg taccgagccg acgttaaaca ccgccattcc gggcgatccg      540

cgtgatacca cttcacctcg ggcaatggcg caaactctgc ggaatctgac gctgggtaaa      600

gcattgggcg acagccaacg ggcgcagctg gtgacatgga tgaaaggcaa taccaccggt      660

gcagcgagca ttcaggctgg actgcctgct tcctgggttg tgggggataa aaccggcagc      720

ggtggctatg gcaccaccaa cgatatcgcg gtgatctggc caaaagatcg tgcgccgctg      780

attctggtca cttacttcac ccagcctcaa cctaaggcag aaagccgtcg cgatgtatta      840

gcgtcggcgg ctaaaatcgt caccgacggt ttgtaa                                876


<210>  6
<211>  291
<212>  PRT
<213>  Escherichia coli

<400>  6

Met Val Lys Lys Ser Leu Arg Gln Phe Thr Leu Met Ala Thr Ala Thr 
1               5                   10                  15      


Val Thr Leu Leu Leu Gly Ser Val Pro Leu Tyr Ala Gln Thr Ala Asp 
            20                  25                  30          


Val Gln Gln Lys Leu Ala Glu Leu Glu Arg Gln Ser Gly Gly Arg Leu 
        35                  40                  45              


Gly Val Ala Leu Ile Asn Thr Ala Asp Asn Ser Gln Ile Leu Tyr Arg 
    50                  55                  60                  


Ala Asp Glu Arg Phe Ala Met Cys Ser Thr Ser Lys Val Met Ala Ala 
65                  70                  75                  80  


Ala Ala Val Leu Lys Lys Ser Glu Ser Glu Pro Asn Leu Leu Asn Gln 
                85                  90                  95      


Arg Val Glu Ile Lys Lys Ser Asp Leu Val Asn Tyr Asn Pro Ile Ala 
            100                 105                 110         


Glu Lys His Val Asn Gly Thr Met Ser Leu Ala Glu Leu Ser Ala Ala 
        115                 120                 125             


Ala Leu Gln Tyr Ser Asp Asn Val Ala Met Asn Lys Leu Ile Ala His 
    130                 135                 140                 


Val Gly Gly Pro Ala Ser Val Thr Ala Phe Ala Arg Gln Leu Gly Asp 
145                 150                 155                 160 


Glu Thr Phe Arg Leu Asp Arg Thr Glu Pro Thr Leu Asn Thr Ala Ile 
                165                 170                 175     


Pro Gly Asp Pro Arg Asp Thr Thr Ser Pro Arg Ala Met Ala Gln Thr 
            180                 185                 190         


Leu Arg Asn Leu Thr Leu Gly Lys Ala Leu Gly Asp Ser Gln Arg Ala 
        195                 200                 205             


Gln Leu Val Thr Trp Met Lys Gly Asn Thr Thr Gly Ala Ala Ser Ile 
    210                 215                 220                 


Gln Ala Gly Leu Pro Ala Ser Trp Val Val Gly Asp Lys Thr Gly Ser 
225                 230                 235                 240 


Gly Gly Tyr Gly Thr Thr Asn Asp Ile Ala Val Ile Trp Pro Lys Asp 
                245                 250                 255     


Arg Ala Pro Leu Ile Leu Val Thr Tyr Phe Thr Gln Pro Gln Pro Lys 
            260                 265                 270         


Ala Glu Ser Arg Arg Asp Val Leu Ala Ser Ala Ala Lys Ile Val Thr 
        275                 280                 285             


Asp Gly Leu 
    290     


<210>  7
<211>  2315
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleic acid sequence of the Escherichia coli blaCTX-M-15 gene 
       preceded by the associated upstream insertional element ISEcp1

<400>  7
tgggattttt gattttattg aaaatgacct cgtatttgat aatgactcaa caaataaaat       60

caagatgaat catataaaga ccatgctctg cggtcacttc attggcattg ataagttaga      120

acgtctaaag ctacttcaaa atgatcccct cgtcaacgag tttgatattt ccgtaaaaga      180

acctgaaaca gtgtcacggt ttctaggaaa cttcaacttc aagacaaccc aaatgtttag      240

agacattaat tttaaagtct ttaaaaaact gctcactaaa agtaaattga catccattac      300

gattgatatt gatagtagtg taattaacgt agaaggtcat caagaaggtg cgtcaaaagg      360

atataatcct aagaaactgg gaaaccgatg ctacaatatc caatttgcat tttgcgacga      420

attaaaagca tatgttaccg gatttgtaag aagtggcaat acttacactg caaacggtgc      480

tgcggaaatg atcaaagaaa ttgttgctaa catcaaatca gacgatttag aaattttatt      540

tcgaatggat agtggctact ttgatgaaaa aattatcgaa acgatagaat ctcttggatg      600

caaatattta attaaagcca aaagttattc tacactcacc tcacaagcaa cgaattcatc      660

aattgtattc gttaaaggag aagaaggtag agaaactaca gaactgtata caaaattagt      720

taaatgggaa aaagacagaa gatttgtcgt atctcgcgta ctgaaaccag aaaaagaaag      780

agcacaatta tcacttttag aaggttccga atacgactac tttttctttg taacaaatac      840

taccttgctt tctgaaaaag tagttatata ctatgaaaag cgtggtaatg ctgaaaacta      900

tatcaaagaa gccaaatacg acatggcggt gggtcatctc ttgctaaagt cattttgggc      960

gaatgaagcc gtgtttcaaa tgatgatgct ttcatataac ctatttttgt tgttcaagtt     1020

tgattccttg gactcttcag aatacagaca gcaaataaag acctttcgtt tgaagtatgt     1080

atttcttgca gcaaaaataa tcaaaaccgc aagatatgta atcatgaagt tgtcggaaaa     1140

ctatccgtac aagggagtgt atgaaaaatg tctggtataa taagaatatc atcaataaaa     1200

ttgagtgttg ctctgtggat aacttgcaga gtttattaag tatcattgca gcaaagatga     1260

aatcaatgat ttatcaaaaa tgattgaaag gtggttgtaa ataatgttac aatgtgtgag     1320

aagcagtcta aattcttcgt gaaatagtga tttttgaagc taataaaaaa cacacgtgga     1380

atttagggac tattcatgtt gttgttattt cgtatcttcc agaataagga atcccatggt     1440

taaaaaatca ctgcgccagt tcacgctgat ggcgacggca accgtcacgc tgttgttagg     1500

aagtgtgccg ctgtatgcgc aaacggcgga cgtacagcaa aaacttgccg aattagagcg     1560

gcagtcggga ggcagactgg gtgtggcatt gattaacaca gcagataatt cgcaaatact     1620

ttatcgtgct gatgagcgct ttgcgatgtg cagcaccagt aaagtgatgg ccgcggccgc     1680

ggtgctgaag aaaagtgaaa gcgaaccgaa tctgttaaat cagcgagttg agatcaaaaa     1740

atctgacctt gttaactata atccgattgc ggaaaagcac gtcaatggga cgatgtcact     1800

ggctgagctt agcgcggccg cgctacagta cagcgataac gtggcgatga ataagctgat     1860

tgctcacgtt ggcggcccgg ctagcgtcac cgcgttcgcc cgacagctgg gagacgaaac     1920

gttccgtctc gaccgtaccg agccgacgtt aaacaccgcc attccgggcg atccgcgtga     1980

taccacttca cctcgggcaa tggcgcaaac tctgcggaat ctgacgctgg gtaaagcatt     2040

gggcgacagc caacgggcgc agctggtgac atggatgaaa ggcaatacca ccggtgcagc     2100

gagcattcag gctggactgc ctgcttcctg ggttgtgggg gataaaaccg gcagcggtgg     2160

ctatggcacc accaacgata tcgcggtgat ctggccaaaa gatcgtgcgc cgctgattct     2220

ggtcacttac ttcacccagc ctcaacctaa ggcagaaagc cgtcgcgatg tattagcgtc     2280

ggcggctaaa atcgtcaccg acggtttgta atagg                                2315


<210>  8
<211>  917
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleic acid sequence of the Escherichia coli blaCTX-M-15 gene by
       the 41 bases of the associated upstream insertional element 
       ISEcp1 directly preceding the blaCTX-M-15 gene

<400>  8
catgttgttg ttatttcgta tcttccagaa taaggaatcc catggttaaa aaatcactgc       60

gccagttcac gctgatggcg acggcaaccg tcacgctgtt gttaggaagt gtgccgctgt      120

atgcgcaaac ggcggacgta cagcaaaaac ttgccgaatt agagcggcag tcgggaggca      180

gactgggtgt ggcattgatt aacacagcag ataattcgca aatactttat cgtgctgatg      240

agcgctttgc gatgtgcagc accagtaaag tgatggccgc ggccgcggtg ctgaagaaaa      300

gtgaaagcga accgaatctg ttaaatcagc gagttgagat caaaaaatct gaccttgtta      360

actataatcc gattgcggaa aagcacgtca atgggacgat gtcactggct gagcttagcg      420

cggccgcgct acagtacagc gataacgtgg cgatgaataa gctgattgct cacgttggcg      480

gcccggctag cgtcaccgcg ttcgcccgac agctgggaga cgaaacgttc cgtctcgacc      540

gtaccgagcc gacgttaaac accgccattc cgggcgatcc gcgtgatacc acttcacctc      600

gggcaatggc gcaaactctg cggaatctga cgctgggtaa agcattgggc gacagccaac      660

gggcgcagct ggtgacatgg atgaaaggca ataccaccgg tgcagcgagc attcaggctg      720

gactgcctgc ttcctgggtt gtgggggata aaaccggcag cggtggctat ggcaccacca      780

acgatatcgc ggtgatctgg ccaaaagatc gtgcgccgct gattctggtc acttacttca      840

cccagcctca acctaaggca gaaagccgtc gcgatgtatt agcgtcggcg gctaaaatcg      900

tcaccgacgg tttgtaa                                                     917


<210>  9
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LONcontrol

<400>  9
ttagttgggg ttcagttgg                                                    19


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the LASOepsilon antisense oligonucleotide

<400>  10
cggcacactt cctaacaaca                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the LASOdzeta antisense oligonucleotide

<400>  11
acggtcgaga cggaacgttt                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the LASOeta antisense oligonucleotide

<400>  12
aggctgggtg aagtaagtga                                                   20


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the gapmer LNA-5'LASOalpha and of the 
       gapmer LNA-ASOalpha antisense oligonucleotides

<400>  13
gcgcagtgat tttttaacca tggga                                             25


