                               SEQUENCE LISTING

<110> CREATICS LLC
 
<120> METHODS FOR CANCER DETECTION BY EVALUATION OF ALPHA-1-ANTITRYPSIN
      (AAT) AND CHYMOTRYPSIN-LIKE ELASTASE IN GASTROINTESTINAL LAVAGE 
      FLUID SAMPLES

<130> 121940-00420

<140> PCT/US2020/031062
<141> 2020-05-01

<150> 62/842,135
<151> 2019-05-02

<160> 7     

<170> PatentIn version 3.5

<210> 1
<211> 418
<212> PRT
<213> Homo sapiens

<400> 1
Met Pro Ser Ser Val Ser Trp Gly Ile Leu Leu Leu Ala Gly Leu Cys 
1               5                   10                  15      


Cys Leu Val Pro Val Ser Leu Ala Glu Asp Pro Gln Gly Asp Ala Ala 
            20                  25                  30          


Gln Lys Thr Asp Thr Ser His His Asp Gln Asp His Pro Thr Phe Asn 
        35                  40                  45              


Lys Ile Thr Pro Asn Leu Ala Glu Phe Ala Phe Ser Leu Tyr Arg Gln 
    50                  55                  60                  


Leu Ala His Gln Ser Asn Ser Thr Asn Ile Phe Phe Ser Pro Val Ser 
65                  70                  75                  80  


Ile Ala Thr Ala Phe Ala Met Leu Ser Leu Gly Thr Lys Ala Asp Thr 
                85                  90                  95      


His Asp Glu Ile Leu Glu Gly Leu Asn Phe Asn Leu Thr Glu Ile Pro 
            100                 105                 110         


Glu Ala Gln Ile His Glu Gly Phe Gln Glu Leu Leu Arg Thr Leu Asn 
        115                 120                 125             


Gln Pro Asp Ser Gln Leu Gln Leu Thr Thr Gly Asn Gly Leu Phe Leu 
    130                 135                 140                 


Ser Glu Gly Leu Lys Leu Val Asp Lys Phe Leu Glu Asp Val Lys Lys 
145                 150                 155                 160 


Leu Tyr His Ser Glu Ala Phe Thr Val Asn Phe Gly Asp Thr Glu Glu 
                165                 170                 175     


Ala Lys Lys Gln Ile Asn Asp Tyr Val Glu Lys Gly Thr Gln Gly Lys 
            180                 185                 190         


Ile Val Asp Leu Val Lys Glu Leu Asp Arg Asp Thr Val Phe Ala Leu 
        195                 200                 205             


Val Asn Tyr Ile Phe Phe Lys Gly Lys Trp Glu Arg Pro Phe Glu Val 
    210                 215                 220                 


Lys Asp Thr Glu Glu Glu Asp Phe His Val Asp Gln Val Thr Thr Val 
225                 230                 235                 240 


Lys Val Pro Met Met Lys Arg Leu Gly Met Phe Asn Ile Gln His Cys 
                245                 250                 255     


Lys Lys Leu Ser Ser Trp Val Leu Leu Met Lys Tyr Leu Gly Asn Ala 
            260                 265                 270         


Thr Ala Ile Phe Phe Leu Pro Asp Glu Gly Lys Leu Gln His Leu Glu 
        275                 280                 285             


Asn Glu Leu Thr His Asp Ile Ile Thr Lys Phe Leu Glu Asn Glu Asp 
    290                 295                 300                 


Arg Arg Ser Ala Ser Leu His Leu Pro Lys Leu Ser Ile Thr Gly Thr 
305                 310                 315                 320 


Tyr Asp Leu Lys Ser Val Leu Gly Gln Leu Gly Ile Thr Lys Val Phe 
                325                 330                 335     


Ser Asn Gly Ala Asp Leu Ser Gly Val Thr Glu Glu Ala Pro Leu Lys 
            340                 345                 350         


Leu Ser Lys Ala Val His Lys Ala Val Leu Thr Ile Asp Glu Lys Gly 
        355                 360                 365             


Thr Glu Ala Ala Gly Ala Met Phe Leu Glu Ala Ile Pro Met Ser Ile 
    370                 375                 380                 


Pro Pro Glu Val Lys Phe Asn Lys Pro Phe Val Phe Leu Met Ile Glu 
385                 390                 395                 400 


Gln Asn Thr Lys Ser Pro Leu Phe Met Gly Lys Val Val Asn Pro Thr 
                405                 410                 415     


Gln Lys 
        


<210> 2
<211> 269
<212> PRT
<213> Homo sapiens

<400> 2
Met Ile Arg Thr Leu Leu Leu Ser Thr Leu Val Ala Gly Ala Leu Ser 
1               5                   10                  15      


Cys Gly Asp Pro Thr Tyr Pro Pro Tyr Val Thr Arg Val Val Gly Gly 
            20                  25                  30          


Glu Glu Ala Arg Pro Asn Ser Trp Pro Trp Gln Val Ser Leu Gln Tyr 
        35                  40                  45              


Ser Ser Asn Gly Lys Trp Tyr His Thr Cys Gly Gly Ser Leu Ile Ala 
    50                  55                  60                  


Asn Ser Trp Val Leu Thr Ala Ala His Cys Ile Ser Ser Ser Arg Thr 
65                  70                  75                  80  


Tyr Arg Val Gly Leu Gly Arg His Asn Leu Tyr Val Ala Glu Ser Gly 
                85                  90                  95      


Ser Leu Ala Val Ser Val Ser Lys Ile Val Val His Lys Asp Trp Asn 
            100                 105                 110         


Ser Asn Gln Ile Ser Lys Gly Asn Asp Ile Ala Leu Leu Lys Leu Ala 
        115                 120                 125             


Asn Pro Val Ser Leu Thr Asp Lys Ile Gln Leu Ala Cys Leu Pro Pro 
    130                 135                 140                 


Ala Gly Thr Ile Leu Pro Asn Asn Tyr Pro Cys Tyr Val Thr Gly Trp 
145                 150                 155                 160 


Gly Arg Leu Gln Thr Asn Gly Ala Val Pro Asp Val Leu Gln Gln Gly 
                165                 170                 175     


Arg Leu Leu Val Val Asp Tyr Ala Thr Cys Ser Ser Ser Ala Trp Trp 
            180                 185                 190         


Gly Ser Ser Val Lys Thr Ser Met Ile Cys Ala Gly Gly Asp Gly Val 
        195                 200                 205             


Ile Ser Ser Cys Asn Gly Asp Ser Gly Gly Pro Leu Asn Cys Gln Ala 
    210                 215                 220                 


Ser Asp Gly Arg Trp Gln Val His Gly Ile Val Ser Phe Gly Ser Arg 
225                 230                 235                 240 


Leu Gly Cys Asn Tyr Tyr His Lys Pro Ser Val Phe Thr Arg Val Ser 
                245                 250                 255     


Asn Tyr Ile Asp Trp Ile Asn Ser Val Ile Ala Asn Asn 
            260                 265                 


<210> 3
<211> 36
<212> PRT
<213> Homo sapiens

<400> 3
Ser Ile Pro Pro Glu Val Lys Phe Asn Lys Pro Phe Val Phe Leu Met 
1               5                   10                  15      


Ile Glu Gln Asn Thr Lys Ser Pro Leu Phe Met Gly Lys Val Val Asn 
            20                  25                  30          


Pro Thr Gln Lys 
        35      


<210> 4
<211> 14
<212> PRT
<213> Homo sapiens

<400> 4
Leu Gly Cys Asn Tyr Tyr His Lys Pro Ser Val Phe Thr Arg 
1               5                   10                  


<210> 5
<211> 270
<212> PRT
<213> Homo sapiens

<400> 5
Met Met Leu Arg Leu Leu Ser Ser Leu Leu Leu Val Ala Val Ala Ser 
1               5                   10                  15      


Gly Tyr Gly Pro Pro Ser Ser His Ser Ser Ser Arg Val Val His Gly 
            20                  25                  30          


Glu Asp Ala Val Pro Tyr Ser Trp Pro Trp Gln Val Ser Leu Gln Tyr 
        35                  40                  45              


Glu Lys Ser Gly Ser Phe Tyr His Thr Cys Gly Gly Ser Leu Ile Ala 
    50                  55                  60                  


Pro Asp Trp Val Val Thr Ala Gly His Cys Ile Ser Arg Asp Leu Thr 
65                  70                  75                  80  


Tyr Gln Val Val Leu Gly Glu Tyr Asn Leu Ala Val Lys Glu Gly Pro 
                85                  90                  95      


Glu Gln Val Ile Pro Ile Asn Ser Glu Glu Leu Phe Val His Pro Leu 
            100                 105                 110         


Trp Asn Arg Ser Cys Val Ala Cys Gly Asn Asp Ile Ala Leu Ile Lys 
        115                 120                 125             


Leu Ser Arg Ser Ala Gln Leu Gly Asp Ala Val Gln Leu Ala Ser Leu 
    130                 135                 140                 


Pro Pro Ala Gly Asp Ile Leu Pro Asn Lys Thr Pro Cys Tyr Ile Thr 
145                 150                 155                 160 


Gly Trp Gly Arg Leu Tyr Thr Asn Gly Pro Leu Pro Asp Lys Leu Gln 
                165                 170                 175     


Gln Ala Arg Leu Pro Val Val Asp Tyr Lys His Cys Ser Arg Trp Asn 
            180                 185                 190         


Trp Trp Gly Ser Thr Val Lys Lys Thr Met Val Cys Ala Gly Gly Tyr 
        195                 200                 205             


Ile Arg Ser Gly Cys Asn Gly Asp Ser Gly Gly Pro Leu Asn Cys Pro 
    210                 215                 220                 


Thr Glu Asp Gly Gly Trp Gln Val His Gly Val Thr Ser Phe Val Ser 
225                 230                 235                 240 


Ala Phe Gly Cys Asn Phe Ile Trp Lys Pro Thr Val Phe Thr Arg Val 
                245                 250                 255     


Ser Ala Phe Ile Asp Trp Ile Glu Glu Thr Ile Ala Ser His 
            260                 265                 270 


<210> 6
<211> 270
<212> PRT
<213> Homo sapiens

<400> 6
Met Met Leu Arg Leu Leu Ser Ser Leu Leu Leu Val Ala Val Ala Ser 
1               5                   10                  15      


Gly Tyr Gly Pro Pro Ser Ser Arg Pro Ser Ser Arg Val Val Asn Gly 
            20                  25                  30          


Glu Asp Ala Val Pro Tyr Ser Trp Pro Trp Gln Val Ser Leu Gln Tyr 
        35                  40                  45              


Glu Lys Ser Gly Ser Phe Tyr His Thr Cys Gly Gly Ser Leu Ile Ala 
    50                  55                  60                  


Pro Asp Trp Val Val Thr Ala Gly His Cys Ile Ser Ser Ser Arg Thr 
65                  70                  75                  80  


Tyr Gln Val Val Leu Gly Glu Tyr Asp Arg Ala Val Lys Glu Gly Pro 
                85                  90                  95      


Glu Gln Val Ile Pro Ile Asn Ser Gly Asp Leu Phe Val His Pro Leu 
            100                 105                 110         


Trp Asn Arg Ser Cys Val Ala Cys Gly Asn Asp Ile Ala Leu Ile Lys 
        115                 120                 125             


Leu Ser Arg Ser Ala Gln Leu Gly Asp Ala Val Gln Leu Ala Ser Leu 
    130                 135                 140                 


Pro Pro Ala Gly Asp Ile Leu Pro Asn Glu Thr Pro Cys Tyr Ile Thr 
145                 150                 155                 160 


Gly Trp Gly Arg Leu Tyr Thr Asn Gly Pro Leu Pro Asp Lys Leu Gln 
                165                 170                 175     


Glu Ala Leu Leu Pro Val Val Asp Tyr Glu His Cys Ser Arg Trp Asn 
            180                 185                 190         


Trp Trp Gly Ser Ser Val Lys Lys Thr Met Val Cys Ala Gly Gly Asp 
        195                 200                 205             


Ile Arg Ser Gly Cys Asn Gly Asp Ser Gly Gly Pro Leu Asn Cys Pro 
    210                 215                 220                 


Thr Glu Asp Gly Gly Trp Gln Val His Gly Val Thr Ser Phe Val Ser 
225                 230                 235                 240 


Ala Phe Gly Cys Asn Thr Arg Arg Lys Pro Thr Val Phe Thr Arg Val 
                245                 250                 255     


Ser Ala Phe Ile Asp Trp Ile Glu Glu Thr Ile Ala Ser His 
            260                 265                 270 


<210> 7
<211> 37
<212> PRT
<213> Homo sapiens

<400> 7
Met Ser Ile Pro Pro Glu Val Lys Phe Asn Lys Pro Phe Val Phe Leu 
1               5                   10                  15      


Met Ile Glu Gln Asn Thr Lys Ser Pro Leu Phe Met Gly Lys Val Val 
            20                  25                  30          


Asn Pro Thr Gln Lys 
        35          

