                         SEQUENCE LISTING

<110>  Conagen Inc.
 
<120>  Biosynthesis of Vanillin from Isoeugenol

<130>  CCN-1207WO00

<150>  US 62/840,284
<151>  2019-04-29

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  1620
<212>  DNA
<213>  Colletotrichum fioriniae

<400>  1
atggcaccgg gtcgtaccga tgatgaagca gcagaagttg caaccagcag caaaacccat       60

gcaattagca attatggtct gaccaccaaa tggcctgaag catacgatct ggcaggtagc      120

aatctgccgt gtcgtctgga aggtgaaatt ggtgatctgg ttgttctggg tgaaattccg      180

aaagaaattg atggcacctt ttatcgtgtt atgaccgatc cgtttgttcc gcctcatccg      240

aataatgttc cgctggatgg tgatggtaat attagcgcat ttcgcattaa agatggtcgc      300

gtggatatga aaatgcgtta tgttgaaacc gagcgctata aactggaacg caaagcaaat      360

aaagcactgt ttggtctgta tcgcaatccg tttacacatc atccgtgtgt tcgtgcagca      420

gttgatagca ccgcaaatac caatgttgtt ctgtgggcag atcattttct ggcactgaaa      480

gaaggtggtc tgccgtatag cgttgatccg cagacactgg aaaccatcaa atatgatcct      540

tttggccaga tcaaagccaa aacctttacc gcacatccga aaattgatcc gtataccaat      600

gaactggtgg tgtttggtta tgaagcacgt ggtctggcaa gcctggatat tgttatttat      660

gcactggata gcaacggcgt gaaacatgat gaacagtgga ttaaaagccc gtggtgtgca      720

ccgattcatg attgtgcaat taccccgaat tggattattc tggccctgtg gccgtttgaa      780

gcaagcgtgg atcgtatgaa aaaaggcggt catcattggg catgggatta tgatttaccg      840

gcaaccttta ttgttgcacc gcgtcgtagc agcaccaaac tgcctccagg ttggcagcct      900

ggtgaaagcc gtgtttatag ctggaaaaat tgcatgctga ttcataccgg tggtgcatgg      960

gaaagcgaag atggtcagac actgtttatg gaaaccacac gtgttcatga taatggcttt     1020

ccgttttttc cgaccaatga aggtgtttat ccggcacctg atccgaaaag cgattatgtt     1080

cgttggacat ttgatctgag tcagccgacc aatagcaaag ttcctgatcc ggaaattatc     1140

ctggatatgc ctgcagaatt tccgcgtctg gatgaacgtt ttctgagcaa aagctataat     1200

tatgtgtgga tcaacgtgta catccacgaa gatcagaacg gtgatggtca taccattgtt     1260

gaaggtctga atggtctggc catgcataat agcaaaacca aagaaaccaa atgctttaat     1320

gccggtgccg aaagtttttg tcaagaaccg atctttattc cgcgtagtga tgatgcaccg     1380

gaaggtgatg gttgggttat gacaatggtt gaacgtcgtg gtgcaaatcg taatgaaatt     1440

gttgtgctgg ataccaagga tttcgaaaaa ccgattgcag ttattcagct gccgtttcat     1500

gttaaagcac aggttcatgg taattgggtt gatcagcgtc gtctgaaagc atggcagaaa     1560

ctggcacgtg atgcagtgcc ggaaccgatt agcggtcagg gtgcactgga accgctgtaa     1620


<210>  2
<211>  539
<212>  PRT
<213>  Colletotrichum fioriniae

<400>  2

Met Ala Pro Gly Arg Thr Asp Asp Glu Ala Ala Glu Val Ala Thr Ser 
1               5                   10                  15      


Ser Lys Thr His Ala Ile Ser Asn Tyr Gly Leu Thr Thr Lys Trp Pro 
            20                  25                  30          


Glu Ala Tyr Asp Leu Ala Gly Ser Asn Leu Pro Cys Arg Leu Glu Gly 
        35                  40                  45              


Glu Ile Gly Asp Leu Val Val Leu Gly Glu Ile Pro Lys Glu Ile Asp 
    50                  55                  60                  


Gly Thr Phe Tyr Arg Val Met Thr Asp Pro Phe Val Pro Pro His Pro 
65                  70                  75                  80  


Asn Asn Val Pro Leu Asp Gly Asp Gly Asn Ile Ser Ala Phe Arg Ile 
                85                  90                  95      


Lys Asp Gly Arg Val Asp Met Lys Met Arg Tyr Val Glu Thr Glu Arg 
            100                 105                 110         


Tyr Lys Leu Glu Arg Lys Ala Asn Lys Ala Leu Phe Gly Leu Tyr Arg 
        115                 120                 125             


Asn Pro Phe Thr His His Pro Cys Val Arg Ala Ala Val Asp Ser Thr 
    130                 135                 140                 


Ala Asn Thr Asn Val Val Leu Trp Ala Asp His Phe Leu Ala Leu Lys 
145                 150                 155                 160 


Glu Gly Gly Leu Pro Tyr Ser Val Asp Pro Gln Thr Leu Glu Thr Ile 
                165                 170                 175     


Lys Tyr Asp Pro Phe Gly Gln Ile Lys Ala Lys Thr Phe Thr Ala His 
            180                 185                 190         


Pro Lys Ile Asp Pro Tyr Thr Asn Glu Leu Val Val Phe Gly Tyr Glu 
        195                 200                 205             


Ala Arg Gly Leu Ala Ser Leu Asp Ile Val Ile Tyr Ala Leu Asp Ser 
    210                 215                 220                 


Asn Gly Val Lys His Asp Glu Gln Trp Ile Lys Ser Pro Trp Cys Ala 
225                 230                 235                 240 


Pro Ile His Asp Cys Ala Ile Thr Pro Asn Trp Ile Ile Leu Ala Leu 
                245                 250                 255     


Trp Pro Phe Glu Ala Ser Val Asp Arg Met Lys Lys Gly Gly His His 
            260                 265                 270         


Trp Ala Trp Asp Tyr Asp Leu Pro Ala Thr Phe Ile Val Ala Pro Arg 
        275                 280                 285             


Arg Ser Ser Thr Lys Leu Pro Pro Gly Trp Gln Pro Gly Glu Ser Arg 
    290                 295                 300                 


Val Tyr Ser Trp Lys Asn Cys Met Leu Ile His Thr Gly Gly Ala Trp 
305                 310                 315                 320 


Glu Ser Glu Asp Gly Gln Thr Leu Phe Met Glu Thr Thr Arg Val His 
                325                 330                 335     


Asp Asn Gly Phe Pro Phe Phe Pro Thr Asn Glu Gly Val Tyr Pro Ala 
            340                 345                 350         


Pro Asp Pro Lys Ser Asp Tyr Val Arg Trp Thr Phe Asp Leu Ser Gln 
        355                 360                 365             


Pro Thr Asn Ser Lys Val Pro Asp Pro Glu Ile Ile Leu Asp Met Pro 
    370                 375                 380                 


Ala Glu Phe Pro Arg Leu Asp Glu Arg Phe Leu Ser Lys Ser Tyr Asn 
385                 390                 395                 400 


Tyr Val Trp Ile Asn Val Tyr Ile His Glu Asp Gln Asn Gly Asp Gly 
                405                 410                 415     


His Thr Ile Val Glu Gly Leu Asn Gly Leu Ala Met His Asn Ser Lys 
            420                 425                 430         


Thr Lys Glu Thr Lys Cys Phe Asn Ala Gly Ala Glu Ser Phe Cys Gln 
        435                 440                 445             


Glu Pro Ile Phe Ile Pro Arg Ser Asp Asp Ala Pro Glu Gly Asp Gly 
    450                 455                 460                 


Trp Val Met Thr Met Val Glu Arg Arg Gly Ala Asn Arg Asn Glu Ile 
465                 470                 475                 480 


Val Val Leu Asp Thr Lys Asp Phe Glu Lys Pro Ile Ala Val Ile Gln 
                485                 490                 495     


Leu Pro Phe His Val Lys Ala Gln Val His Gly Asn Trp Val Asp Gln 
            500                 505                 510         


Arg Arg Leu Lys Ala Trp Gln Lys Leu Ala Arg Asp Ala Val Pro Glu 
        515                 520                 525             


Pro Ile Ser Gly Gln Gly Ala Leu Glu Pro Leu 
    530                 535                 


<210>  3
<211>  1437
<212>  DNA
<213>  Pseudomonas putida

<400>  3
atggcgagac tcaaccgcaa cgacccgcaa ttagtaggaa cacttctccc cacccgtatc       60

gaggcagact tgttcgatct agaggttgac ggcgaaatcc caaaatcaat aaatggaacg      120

ttctaccgta atacgccaga acctcaagtt accccgcaaa aattccacac cttcatagat      180

ggagatggaa tggcctctgc cttccacttc gaagatggtc atgtcgactt catcagtcgc      240

tgggttaaaa ccgctcgatt cacggccgaa cgactagcgc gaaaatcgct atttggcatg      300

tacagaaacc cctataccga cgacaccagt gtaaaaggac tagaccgcac cgttgccaat      360

acaagcatca ttagccatca cggcaaggtg ctggcggtga aggaagacgg cctaccgtac      420

gaactggatc ctcgtacact tgaaactcgc ggacgcttcg actacgacgg ccaagttacc      480

agccaaaccc acaccgccca tccaaaatat gacccggaaa cgggtgactt gttgttcttc      540

ggttcggcag ctaagggcga agcaactcca gacatggcct attacattgt cgacaagcac      600

ggcaaggtga cacatgaaac ttggtttgag cagccctatg gcgcattcat gcacgacttt      660

gccattaccc gaaattggtc cattttccca attatgccgg ccaccaacag cctgtcccgc      720

ctcaaggcga aacagccaat ttatatgtgg gagccggaac tgggcagcta cattggcgta      780

ctgccgcgcc gcggccaggg cagtcagatt cgctggctca aggcaccggc gctctgggta      840

tttcatgttg tgaatgcttg ggaagtcgga accaagattt atatcgacct tatggaaagt      900

gaaatcctgc cgttcccctt ccccaactca caaaaccaac ccttcgcccc tgagaaagcc      960

gtaccacgcc tgactcgttg ggaaattgac ctcgatagca gcagcgacga gatcaagcga     1020

acccggctac acgatttctt tgcggaaatg ccaatcatgg attttcgctt cgccctgcaa     1080

tgcaaccgct atggctttat gggggtggac gatccacgca aaccacttgc gcatcagcag     1140

gccgagaaga tatttgcgta caactcactc ggcatctggg acaaccaccg aggtgactac     1200

gacctctggt actccggaga agcctcggcg gcccaggagc cggccttcgt ccctagaagt     1260

ccgaccgccg ccgaaggtga tgggtacttg ctgaccgtgg ttggtcgtct cgatgaaaat     1320

cgcagtgatc tggtaattct cgacactcaa gacatccagt ctggtcccgt ggcaaccatc     1380

aagctgccat tccggttaag ggccgctctc catggctgct gggtacccag accttaa        1437


<210>  4
<211>  478
<212>  PRT
<213>  Pseudomonas putida

<400>  4

Met Ala Thr Phe Asp Arg Asn Asp Pro Gln Leu Ala Gly Thr Met Phe 
1               5                   10                  15      


Pro Thr Arg Ile Glu Ala Asn Val Phe Asp Leu Glu Ile Glu Gly Glu 
            20                  25                  30          


Ile Pro Arg Ala Ile Asn Gly Ser Phe Phe Arg Asn Thr Pro Glu Pro 
        35                  40                  45              


Gln Val Thr Thr Gln Pro Phe His Thr Phe Ile Asp Gly Asp Gly Leu 
    50                  55                  60                  


Ala Ser Ala Phe His Phe Glu Asp Gly Gln Val Asp Phe Val Ser Arg 
65                  70                  75                  80  


Trp Val Cys Thr Pro Arg Phe Glu Ala Glu Arg Ser Ala Arg Lys Ser 
                85                  90                  95      


Leu Phe Gly Met Tyr Arg Asn Pro Phe Thr Asp Asp Pro Ser Val Glu 
            100                 105                 110         


Gly Ile Asp Arg Thr Val Ala Asn Thr Ser Ile Ile Thr His His Gly 
        115                 120                 125             


Lys Val Leu Ala Ala Lys Glu Asp Gly Leu Pro Tyr Glu Leu Asp Pro 
    130                 135                 140                 


Gln Thr Leu Glu Thr Arg Gly Arg Tyr Asp Tyr Lys Gly Gln Val Thr 
145                 150                 155                 160 


Ser His Thr His Thr Ala His Pro Lys Phe Asp Pro Gln Thr Gly Glu 
                165                 170                 175     


Met Leu Leu Phe Gly Ser Ala Ala Lys Gly Glu Arg Thr Leu Asp Met 
            180                 185                 190         


Ala Tyr Tyr Ile Val Asp Arg Tyr Gly Lys Val Thr His Glu Thr Trp 
        195                 200                 205             


Phe Lys Gln Pro Tyr Gly Ala Phe Met His Asp Phe Ala Val Thr Arg 
    210                 215                 220                 


Asn Trp Ser Ile Phe Pro Ile Met Pro Ala Thr Asn Ser Leu Glu Arg 
225                 230                 235                 240 


Leu Lys Ala Lys Gln Pro Ile Tyr Met Trp Glu Pro Glu Arg Gly Ser 
                245                 250                 255     


Tyr Ile Gly Val Leu Pro Arg Arg Gly Gln Gly Lys Asp Ile Arg Trp 
            260                 265                 270         


Phe Arg Ala Pro Ala Leu Trp Val Phe His Val Val Asn Ala Trp Glu 
        275                 280                 285             


Glu Gly Asn Arg Ile Leu Ile Asp Leu Met Glu Ser Glu Ile Leu Pro 
    290                 295                 300                 


Phe Pro Phe Pro Asn Ser Gln Asn Leu Pro Phe Asp Pro Ser Lys Ala 
305                 310                 315                 320 


Val Pro Arg Leu Thr Arg Trp Glu Ile Asp Leu Asn Ser Gly Asn Asp 
                325                 330                 335     


Glu Met Lys Arg Thr Gln Leu His Glu Tyr Phe Ala Glu Met Pro Ile 
            340                 345                 350         


Met Asp Phe Arg Phe Ala Leu Gln Asp His Arg Tyr Ala Tyr Met Gly 
        355                 360                 365             


Val Asp Asp Pro Arg Arg Pro Leu Ala His Gln Gln Ala Glu Lys Ile 
    370                 375                 380                 


Phe Ala Tyr Asn Ser Leu Gly Val Trp Asp Asn His Arg Lys Asp Tyr 
385                 390                 395                 400 


Glu Leu Trp Phe Thr Gly Lys Met Ser Ala Ala Gln Glu Pro Ala Phe 
                405                 410                 415     


Val Pro Arg Ser Pro Asp Ala Pro Glu Gly Asp Gly Tyr Leu Leu Ser 
            420                 425                 430         


Val Val Gly Arg Leu Asp Glu Asp Arg Ser Asp Leu Val Ile Leu Asp 
        435                 440                 445             


Thr Gln Cys Leu Ala Ala Gly Pro Val Ala Thr Val Lys Leu Pro Phe 
    450                 455                 460                 


Arg Leu Arg Ala Ala Leu His Gly Cys Trp Gln Ser Lys Asn 
465                 470                 475             


<210>  5
<211>  2325
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid

<400>  5
ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc atcgtttagg       60

caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat      120

aacaatttca actataagaa ggagatatac atatggcgga tccgaattcg gcgcgccaga      180

tctcaattgg atatcggccg gccgacgtcg gtaccgcggc cgccaccgct gagcaataac      240

tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa      300

ctatatccgg gtaacgaatt caagcttgat atcattcagg acgagcctca gactccagcg      360

taactggact gcaatcaact cactggctca ccttcacggg tgggcctttc ttcggtagaa      420

aatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa      480

aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc      540

gaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag      600

ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg      660

ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga      720

tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc      780

ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc      840

acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga      900

gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt      960

cgccacctct gacttgagca tcgatttttg tgatgctcgt caggggggcg gagcctatgg     1020

aaaaacgcca gcaacgcaga aaggcccacc cgaaggtgag ccaggtgatt acatttgggc     1080

cctcatcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca     1140

tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg     1200

agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt     1260

ttttcctgtt tggtcattta ccaatgctta atcagtgagg cacctatctc agcgatctgt     1320

ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag     1380

ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca     1440

gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact     1500

ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca     1560

gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg     1620

tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc     1680

atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg     1740

gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca     1800

tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt     1860

atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc     1920

agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc     1980

ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca     2040

tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa     2100

aagggaataa gggcgacacg gaaatgttga atactcatag ctcctgaaaa tctcgataac     2160

tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg     2220

tgccgatcaa gtcaaaagcc tccggtcgga ggcttttgac tttctgctat ggaggtcagg     2280

tatgatttaa atggtcagta ttgagcgata tctagagaat tcgtc                     2325


<210>  6
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
ggaattccat atggcaccgg gtcgtaccga tgatgaagc                              39


<210>  7
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  7
aaggaaaaaa gcggccgcca gcggttccag tgcaccctga cc                          42


<210>  8
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
aaggaaaaaa gcggccgctt acagcggttc cagtgcaccc tgacc                       45


<210>  9
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  9
ggaattccat atgagcgcac cggaagaaca tcatgg                                 36


<210>  10
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  10
aaggaaaaaa gcggccgctg ccagcggttc cagtgcacca cg                          42


<210>  11
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  11
aaggaaaaaa gcggccgctt atgccagcgg ttccagtgca ccacg                       45


