                         SEQUENCE LISTING

<110>  Conagen Inc.
 
<120>  Biosynthesis of Vanillin from Isoeugenol

<130>  CCN-1206WO00

<140>  PCT/US 20/030573
<141>  2020-04-29

<150>  US 62/840,276
<151>  2019-04-29

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1569
<212>  DNA
<213>  Unknown

<220>
<223>  Fusarium fujikuroi

<400>  1
atgagcgcac cggaagaaca tcatggtctg aaaagcaaat ggcctcaggc atttgatctg       60

gcaggtagca atagcccgtg tcgtattgaa ggtgaaattg gtgatctggt tgttctgggt      120

gaaattccgc ctgcaattga tggcaccttt tatcgtgtta tgtgtgatcc gtttgttccg      180

cctcatccgg ataatgttcc gctggatggt gatggtcatg ttagcgcatt tcgtatctat      240

aatggtcgcg tggacatgaa aatcaaatat gttgaaaccg agcgctacaa actggaacgt      300

aaagcaggta aagcactgtt tggtctgtat cgtaatccgt ttacacatca tccgtgtgtt      360

cgtgcagcag ttgatagcac cgcaaatacc aatatggttt attgggcaga tcgtctgctg      420

gcactgaaag aaagcgcact gccgtatgaa atgcatcctg atacactgga aaccctgtgt      480

tatgatcctt ttggtggtca ggttaaagca aaagcattta ccgcacatcc gaaaatcgat      540

ccgtttagtg atgaactggt tgtgtatggt tatgaagcaa aaggtctggc aacccgtgat      600

attgtgattt atagcctgga taaaaacggc atcaaacacg atgaacagtg gattgaaagc      660

ccgtggtgtg caccgattca tgattgtgtt attaccccga actttatcgt tctgattctg      720

tggccgtttg aagcaagcgt tgaacgtatg aaagccggta aacagcattg ggcatgggat      780

tatagtctgc ctgcaacctt tattgttgtt ccgcgtcgta aaaccagcaa aattccggca      840

agctggaaac agggtgaaca tcgtgtgtat cattggaaaa actgcatgaa cattcatgcc      900

ggtagcgcat gggaagaaga taacggtaaa ctgtatctgg aaaccagccg tgttcatgat      960

aatgcctttc cgttttttcc tccggttgat ggtcgtatgc ctgcaccgga tgcaaaagca     1020

gattttgttc gttgggaaat tgatctgaat gcaccgagcg gcacccagat ggcagatccg     1080

gaagttattc tggatgttcc gagcgaattt ccgcgtattg atgaacgttt tatgaccaaa     1140

cgtaacgagt acctgtttct gaatgtgttt attccggaaa ccagtcaagg tggcaccaac     1200

atttttcatg gcctgaatgg tctggccatg cataatcata gcaccggtga aaccaaatgg     1260

ttttttgccg gtaaagatag cctggttcaa gaaccgatct ttattccgcg taccgcagat     1320

gctccggaag gtgatggttg ggttattgca atgctggaac gtcgtgttgc aaatcgtagc     1380

gaactggtgg ttctggatac ccgtgaattt gaaaaaccgg ttgcatttat tcagctgccg     1440

atgcatctga aagcacaggt tcatggtaat tggattgata gtcgtacccg tgcaagcacc     1500

gaagcaattg ttcgtcagat tggtgaagtt aaagttagcg cacgtggtgc actggaaccg     1560

ctggcataa                                                             1569


<210>  2
<211>  522
<212>  PRT
<213>  UNKNOWN

<220>
<223>  Fusarium fujikuroi

<400>  2

Met Ser Ala Pro Glu Glu His His Gly Leu Lys Ser Lys Trp Pro Gln 
1               5                   10                  15      


Ala Phe Asp Leu Ala Gly Ser Asn Ser Pro Cys Arg Ile Glu Gly Glu 
            20                  25                  30          


Ile Gly Asp Leu Val Val Leu Gly Glu Ile Pro Pro Ala Ile Asp Gly 
        35                  40                  45              


Thr Phe Tyr Arg Val Met Cys Asp Pro Phe Val Pro Pro His Pro Asp 
    50                  55                  60                  


Asn Val Pro Leu Asp Gly Asp Gly His Val Ser Ala Phe Arg Ile Tyr 
65                  70                  75                  80  


Asn Gly Arg Val Asp Met Lys Ile Lys Tyr Val Glu Thr Glu Arg Tyr 
                85                  90                  95      


Lys Leu Glu Arg Lys Ala Gly Lys Ala Leu Phe Gly Leu Tyr Arg Asn 
            100                 105                 110         


Pro Phe Thr His His Pro Cys Val Arg Ala Ala Val Asp Ser Thr Ala 
        115                 120                 125             


Asn Thr Asn Met Val Tyr Trp Ala Asp Arg Leu Leu Ala Leu Lys Glu 
    130                 135                 140                 


Ser Ala Leu Pro Tyr Glu Met His Pro Asp Thr Leu Glu Thr Leu Cys 
145                 150                 155                 160 


Tyr Asp Pro Phe Gly Gly Gln Val Lys Ala Lys Ala Phe Thr Ala His 
                165                 170                 175     


Pro Lys Ile Asp Pro Phe Ser Asp Glu Leu Val Val Tyr Gly Tyr Glu 
            180                 185                 190         


Ala Lys Gly Leu Ala Thr Arg Asp Ile Val Ile Tyr Ser Leu Asp Lys 
        195                 200                 205             


Asn Gly Ile Lys His Asp Glu Gln Trp Ile Glu Ser Pro Trp Cys Ala 
    210                 215                 220                 


Pro Ile His Asp Cys Val Ile Thr Pro Asn Phe Ile Val Leu Ile Leu 
225                 230                 235                 240 


Trp Pro Phe Glu Ala Ser Val Glu Arg Met Lys Ala Gly Lys Gln His 
                245                 250                 255     


Trp Ala Trp Asp Tyr Ser Leu Pro Ala Thr Phe Ile Val Val Pro Arg 
            260                 265                 270         


Arg Lys Thr Ser Lys Ile Pro Ala Ser Trp Lys Gln Gly Glu His Arg 
        275                 280                 285             


Val Tyr His Trp Lys Asn Cys Met Asn Ile His Ala Gly Ser Ala Trp 
    290                 295                 300                 


Glu Glu Asp Asn Gly Lys Leu Tyr Leu Glu Thr Ser Arg Val His Asp 
305                 310                 315                 320 


Asn Ala Phe Pro Phe Phe Pro Pro Val Asp Gly Arg Met Pro Ala Pro 
                325                 330                 335     


Asp Ala Lys Ala Asp Phe Val Arg Trp Glu Ile Asp Leu Asn Ala Pro 
            340                 345                 350         


Ser Gly Thr Gln Met Ala Asp Pro Glu Val Ile Leu Asp Val Pro Ser 
        355                 360                 365             


Glu Phe Pro Arg Ile Asp Glu Arg Phe Met Thr Lys Arg Asn Glu Tyr 
    370                 375                 380                 


Leu Phe Leu Asn Val Phe Ile Pro Glu Thr Ser Gln Gly Gly Thr Asn 
385                 390                 395                 400 


Ile Phe His Gly Leu Asn Gly Leu Ala Met His Asn His Ser Thr Gly 
                405                 410                 415     


Glu Thr Lys Trp Phe Phe Ala Gly Lys Asp Ser Leu Val Gln Glu Pro 
            420                 425                 430         


Ile Phe Ile Pro Arg Thr Ala Asp Ala Pro Glu Gly Asp Gly Trp Val 
        435                 440                 445             


Ile Ala Met Leu Glu Arg Arg Val Ala Asn Arg Ser Glu Leu Val Val 
    450                 455                 460                 


Leu Asp Thr Arg Glu Phe Glu Lys Pro Val Ala Phe Ile Gln Leu Pro 
465                 470                 475                 480 


Met His Leu Lys Ala Gln Val His Gly Asn Trp Ile Asp Ser Arg Thr 
                485                 490                 495     


Arg Ala Ser Thr Glu Ala Ile Val Arg Gln Ile Gly Glu Val Lys Val 
            500                 505                 510         


Ser Ala Arg Gly Ala Leu Glu Pro Leu Ala 
        515                 520         


<210>  3
<211>  2325
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid

<400>  3
ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc atcgtttagg       60

caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat      120

aacaatttca actataagaa ggagatatac atatggcgga tccgaattcg gcgcgccaga      180

tctcaattgg atatcggccg gccgacgtcg gtaccgcggc cgccaccgct gagcaataac      240

tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa      300

ctatatccgg gtaacgaatt caagcttgat atcattcagg acgagcctca gactccagcg      360

taactggact gcaatcaact cactggctca ccttcacggg tgggcctttc ttcggtagaa      420

aatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa      480

aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc      540

gaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag      600

ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg      660

ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga      720

tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc      780

ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc      840

acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga      900

gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt      960

cgccacctct gacttgagca tcgatttttg tgatgctcgt caggggggcg gagcctatgg     1020

aaaaacgcca gcaacgcaga aaggcccacc cgaaggtgag ccaggtgatt acatttgggc     1080

cctcatcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca     1140

tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg     1200

agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt     1260

ttttcctgtt tggtcattta ccaatgctta atcagtgagg cacctatctc agcgatctgt     1320

ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag     1380

ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca     1440

gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact     1500

ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca     1560

gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg     1620

tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc     1680

atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg     1740

gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca     1800

tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt     1860

atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc     1920

agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc     1980

ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca     2040

tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa     2100

aagggaataa gggcgacacg gaaatgttga atactcatag ctcctgaaaa tctcgataac     2160

tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg     2220

tgccgatcaa gtcaaaagcc tccggtcgga ggcttttgac tttctgctat ggaggtcagg     2280

tatgatttaa atggtcagta ttgagcgata tctagagaat tcgtc                     2325


<210>  4
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  4
ggaattccat atgagcgcac cggaagaaca tcatggtc                               38


<210>  5
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
ccgctcgagt tatgccagcg gttccagtgc accacg                                 36


<210>  6
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
aaggaaaaaa gcggccgctt atgccagcgg ttccagtgca ccacg                       45


