                         SEQUENCE LISTING

<110>  THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
 
<120>  COMPOSITIONS USEFUL FOR TREATING POMPE DISEASE

<130>  19-8856PCT2

<150>  US 62/913,401
<151>  2019-10-10

<150>  US 62/840,911
<151>  2019-04-30

<160>  31    

<170>  PatentIn version 3.5

<210>  1
<211>  2211
<212>  DNA
<213>  adeno-associated virus hu.68


<220>
<221>  CDS
<222>  (1)..(2211)

<400>  1
atg gct gcc gat ggt tat ctt cca gat tgg ctc gag gac aac ctc agt         48
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser           
1               5                   10                  15                

gaa ggc att cgc gag tgg tgg gct ttg aaa cct gga gcc cct caa ccc         96
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro           
            20                  25                  30                    

aag gca aat caa caa cat caa gac aac gct cgg ggt ctt gtg ctt ccg        144
Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro           
        35                  40                  45                        

ggt tac aaa tac ctt gga ccc ggc aac gga ctc gac aag ggg gag ccg        192
Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro           
    50                  55                  60                            

gtc aac gaa gca gac gcg gcg gcc ctc gag cac gac aag gcc tac gac        240
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp           
65                  70                  75                  80            

cag cag ctc aag gcc gga gac aac ccg tac ctc aag tac aac cac gcc        288
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala           
                85                  90                  95                

gac gcc gag ttc cag gag cgg ctc aaa gaa gat acg tct ttt ggg ggc        336
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly           
            100                 105                 110                   

aac ctc ggg cga gca gtc ttc cag gcc aaa aag agg ctt ctt gaa cct        384
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro           
        115                 120                 125                       

ctt ggt ctg gtt gag gaa gcg gct aag acg gct cct gga aag aag agg        432
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg           
    130                 135                 140                           

cct gta gag cag tct cct cag gaa ccg gac tcc tcc gtg ggt att ggc        480
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Val Gly Ile Gly           
145                 150                 155                 160           

aaa tcg ggt gca cag ccc gct aaa aag aga ctc aat ttc ggt cag act        528
Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr           
                165                 170                 175               

ggc gac aca gag tca gtc ccc gac cct caa cca atc gga gaa cct ccc        576
Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro           
            180                 185                 190                   

gca gcc ccc tca ggt gtg gga tct ctt aca atg gct tca ggt ggt ggc        624
Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly           
        195                 200                 205                       

gca cca gtg gca gac aat aac gaa ggt gcc gat gga gtg ggt agt tcc        672
Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser           
    210                 215                 220                           

tcg gga aat tgg cat tgc gat tcc caa tgg ctg ggg gac aga gtc atc        720
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile           
225                 230                 235                 240           

acc acc agc acc cga acc tgg gcc ctg ccc acc tac aac aat cac ctc        768
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu           
                245                 250                 255               

tac aag caa atc tcc aac agc aca tct gga gga tct tca aat gac aac        816
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn           
            260                 265                 270                   

gcc tac ttc ggc tac agc acc ccc tgg ggg tat ttt gac ttc aac aga        864
Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg           
        275                 280                 285                       

ttc cac tgc cac ttc tca cca cgt gac tgg caa aga ctc atc aac aac        912
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn           
    290                 295                 300                           

aac tgg gga ttc cgg cct aag cga ctc aac ttc aag ctc ttc aac att        960
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile           
305                 310                 315                 320           

cag gtc aaa gag gtt acg gac aac aat gga gtc aag acc atc gct aat       1008
Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn           
                325                 330                 335               

aac ctt acc agc acg gtc cag gtc ttc acg gac tca gac tat cag ctc       1056
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu           
            340                 345                 350                   

ccg tac gtg ctc ggg tcg gct cac gag ggc tgc ctc ccg ccg ttc cca       1104
Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro           
        355                 360                 365                       

gcg gac gtt ttc atg att cct cag tac ggg tat cta acg ctt aat gat       1152
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp           
    370                 375                 380                           

gga agc caa gcc gtg ggt cgt tcg tcc ttt tac tgc ctg gaa tat ttc       1200
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe           
385                 390                 395                 400           

ccg tcg caa atg cta aga acg ggt aac aac ttc cag ttc agc tac gag       1248
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu           
                405                 410                 415               

ttt gag aac gta cct ttc cat agc agc tat gct cac agc caa agc ctg       1296
Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu           
            420                 425                 430                   

gac cga ctc atg aat cca ctc atc gac caa tac ttg tac tat ctc tca       1344
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser           
        435                 440                 445                       

aag act att aac ggt tct gga cag aat caa caa acg cta aaa ttc agt       1392
Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser           
    450                 455                 460                           

gtg gcc gga ccc agc aac atg gct gtc cag gga aga aac tac ata cct       1440
Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro           
465                 470                 475                 480           

gga ccc agc tac cga caa caa cgt gtc tca acc act gtg act caa aac       1488
Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn           
                485                 490                 495               

aac aac agc gaa ttt gct tgg cct gga gct tct tct tgg gct ctc aat       1536
Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn           
            500                 505                 510                   

gga cgt aat agc ttg atg aat cct gga cct gct atg gcc agc cac aaa       1584
Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys           
        515                 520                 525                       

gaa gga gag gac cgt ttc ttt cct ttg tct gga tct tta att ttt ggc       1632
Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly           
    530                 535                 540                           

aaa caa gga act gga aga gac aac gtg gat gcg gac aaa gtc atg ata       1680
Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile           
545                 550                 555                 560           

acc aac gaa gaa gaa att aaa act acc aac cca gta gca acg gag tcc       1728
Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser           
                565                 570                 575               

tat gga caa gtg gcc aca aac cac cag agt gcc caa gca cag gcg cag       1776
Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln           
            580                 585                 590                   

acc ggc tgg gtt caa aac caa gga ata ctt ccg ggt atg gtt tgg cag       1824
Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln           
        595                 600                 605                       

gac aga gat gtg tac ctg caa gga ccc att tgg gcc aaa att cct cac       1872
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His           
    610                 615                 620                           

acg gac ggc aac ttt cac cct tct ccg ctg atg gga ggg ttt gga atg       1920
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met           
625                 630                 635                 640           

aag cac ccg cct cct cag atc ctc atc aaa aac aca cct gta cct gcg       1968
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala           
                645                 650                 655               

gat cct cca acg gct ttc aac aag gac aag ctg aac tct ttc atc acc       2016
Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr           
            660                 665                 670                   

cag tat tct act ggc caa gtc agc gtg gag att gag tgg gag ctg cag       2064
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln           
        675                 680                 685                       

aag gaa aac agc aag cgc tgg aac ccg gag atc cag tac act tcc aac       2112
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn           
    690                 695                 700                           

tat tac aag tct aat aat gtt gaa ttt gct gtt aat act gaa ggt gtt       2160
Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val           
705                 710                 715                 720           

tat tct gaa ccc cgc ccc att ggc acc aga tac ctg act cgt aat ctg       2208
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu           
                725                 730                 735               

taa                                                                   2211


<210>  2
<211>  736
<212>  PRT
<213>  adeno-associated virus hu.68

<400>  2

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro 
            20                  25                  30          


Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Val Gly Ile Gly 
145                 150                 155                 160 


Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 
        195                 200                 205             


Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 
            260                 265                 270         


Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 
        275                 280                 285             


Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 
    290                 295                 300                 


Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 
305                 310                 315                 320 


Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 
                325                 330                 335     


Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 
            340                 345                 350         


Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 
        355                 360                 365             


Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 
    370                 375                 380                 


Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 
385                 390                 395                 400 


Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 
                405                 410                 415     


Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 
            420                 425                 430         


Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 
        435                 440                 445             


Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser 
    450                 455                 460                 


Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro 
465                 470                 475                 480 


Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn 
                485                 490                 495     


Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn 
            500                 505                 510         


Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys 
        515                 520                 525             


Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly 
    530                 535                 540                 


Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile 
545                 550                 555                 560 


Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser 
                565                 570                 575     


Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln 
            580                 585                 590         


Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln 
        595                 600                 605             


Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His 
    610                 615                 620                 


Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met 
625                 630                 635                 640 


Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala 
                645                 650                 655     


Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr 
            660                 665                 670         


Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 
        675                 680                 685             


Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn 
    690                 695                 700                 


Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val 
705                 710                 715                 720 


Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 
                725                 730                 735     


<210>  3
<211>  952
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct


<220>
<221>  MISC_FEATURE
<222>  (1)..(27)
<223>  Signal peptide

<220>
<221>  MISC_FEATURE
<222>  (70)..(952)

<220>
<221>  MISC_FEATURE
<222>  (123)..(952)
<223>  76kD GAA Protein with V780I

<220>
<221>  MISC_FEATURE
<222>  (204)..(952)
<223>  70 kD GAA Protein with V780I

<400>  3

Met Gly Val Arg His Pro Pro Cys Ser His Arg Leu Leu Ala Val Cys 
1               5                   10                  15      


Ala Leu Val Ser Leu Ala Thr Ala Ala Leu Leu Gly His Ile Leu Leu 
            20                  25                  30          


His Asp Phe Leu Leu Val Pro Arg Glu Leu Ser Gly Ser Ser Pro Val 
        35                  40                  45              


Leu Glu Glu Thr His Pro Ala His Gln Gln Gly Ala Ser Arg Pro Gly 
    50                  55                  60                  


Pro Arg Asp Ala Gln Ala His Pro Gly Arg Pro Arg Ala Val Pro Thr 
65                  70                  75                  80  


Gln Cys Asp Val Pro Pro Asn Ser Arg Phe Asp Cys Ala Pro Asp Lys 
                85                  90                  95      


Ala Ile Thr Gln Glu Gln Cys Glu Ala Arg Gly Cys Cys Tyr Ile Pro 
            100                 105                 110         


Ala Lys Gln Gly Leu Gln Gly Ala Gln Met Gly Gln Pro Trp Cys Phe 
        115                 120                 125             


Phe Pro Pro Ser Tyr Pro Ser Tyr Lys Leu Glu Asn Leu Ser Ser Ser 
    130                 135                 140                 


Glu Met Gly Tyr Thr Ala Thr Leu Thr Arg Thr Thr Pro Thr Phe Phe 
145                 150                 155                 160 


Pro Lys Asp Ile Leu Thr Leu Arg Leu Asp Val Met Met Glu Thr Glu 
                165                 170                 175     


Asn Arg Leu His Phe Thr Ile Lys Asp Pro Ala Asn Arg Arg Tyr Glu 
            180                 185                 190         


Val Pro Leu Glu Thr Pro His Val His Ser Arg Ala Pro Ser Pro Leu 
        195                 200                 205             


Tyr Ser Val Glu Phe Ser Glu Glu Pro Phe Gly Val Ile Val Arg Arg 
    210                 215                 220                 


Gln Leu Asp Gly Arg Val Leu Leu Asn Thr Thr Val Ala Pro Leu Phe 
225                 230                 235                 240 


Phe Ala Asp Gln Phe Leu Gln Leu Ser Thr Ser Leu Pro Ser Gln Tyr 
                245                 250                 255     


Ile Thr Gly Leu Ala Glu His Leu Ser Pro Leu Met Leu Ser Thr Ser 
            260                 265                 270         


Trp Thr Arg Ile Thr Leu Trp Asn Arg Asp Leu Ala Pro Thr Pro Gly 
        275                 280                 285             


Ala Asn Leu Tyr Gly Ser His Pro Phe Tyr Leu Ala Leu Glu Asp Gly 
    290                 295                 300                 


Gly Ser Ala His Gly Val Phe Leu Leu Asn Ser Asn Ala Met Asp Val 
305                 310                 315                 320 


Val Leu Gln Pro Ser Pro Ala Leu Ser Trp Arg Ser Thr Gly Gly Ile 
                325                 330                 335     


Leu Asp Val Tyr Ile Phe Leu Gly Pro Glu Pro Lys Ser Val Val Gln 
            340                 345                 350         


Gln Tyr Leu Asp Val Val Gly Tyr Pro Phe Met Pro Pro Tyr Trp Gly 
        355                 360                 365             


Leu Gly Phe His Leu Cys Arg Trp Gly Tyr Ser Ser Thr Ala Ile Thr 
    370                 375                 380                 


Arg Gln Val Val Glu Asn Met Thr Arg Ala His Phe Pro Leu Asp Val 
385                 390                 395                 400 


Gln Trp Asn Asp Leu Asp Tyr Met Asp Ser Arg Arg Asp Phe Thr Phe 
                405                 410                 415     


Asn Lys Asp Gly Phe Arg Asp Phe Pro Ala Met Val Gln Glu Leu His 
            420                 425                 430         


Gln Gly Gly Arg Arg Tyr Met Met Ile Val Asp Pro Ala Ile Ser Ser 
        435                 440                 445             


Ser Gly Pro Ala Gly Ser Tyr Arg Pro Tyr Asp Glu Gly Leu Arg Arg 
    450                 455                 460                 


Gly Val Phe Ile Thr Asn Glu Thr Gly Gln Pro Leu Ile Gly Lys Val 
465                 470                 475                 480 


Trp Pro Gly Ser Thr Ala Phe Pro Asp Phe Thr Asn Pro Thr Ala Leu 
                485                 490                 495     


Ala Trp Trp Glu Asp Met Val Ala Glu Phe His Asp Gln Val Pro Phe 
            500                 505                 510         


Asp Gly Met Trp Ile Asp Met Asn Glu Pro Ser Asn Phe Ile Arg Gly 
        515                 520                 525             


Ser Glu Asp Gly Cys Pro Asn Asn Glu Leu Glu Asn Pro Pro Tyr Val 
    530                 535                 540                 


Pro Gly Val Val Gly Gly Thr Leu Gln Ala Ala Thr Ile Cys Ala Ser 
545                 550                 555                 560 


Ser His Gln Phe Leu Ser Thr His Tyr Asn Leu His Asn Leu Tyr Gly 
                565                 570                 575     


Leu Thr Glu Ala Ile Ala Ser His Arg Ala Leu Val Lys Ala Arg Gly 
            580                 585                 590         


Thr Arg Pro Phe Val Ile Ser Arg Ser Thr Phe Ala Gly His Gly Arg 
        595                 600                 605             


Tyr Ala Gly His Trp Thr Gly Asp Val Trp Ser Ser Trp Glu Gln Leu 
    610                 615                 620                 


Ala Ser Ser Val Pro Glu Ile Leu Gln Phe Asn Leu Leu Gly Val Pro 
625                 630                 635                 640 


Leu Val Gly Ala Asp Val Cys Gly Phe Leu Gly Asn Thr Ser Glu Glu 
                645                 650                 655     


Leu Cys Val Arg Trp Thr Gln Leu Gly Ala Phe Tyr Pro Phe Met Arg 
            660                 665                 670         


Asn His Asn Ser Leu Leu Ser Leu Pro Gln Glu Pro Tyr Ser Phe Ser 
        675                 680                 685             


Glu Pro Ala Gln Gln Ala Met Arg Lys Ala Leu Thr Leu Arg Tyr Ala 
    690                 695                 700                 


Leu Leu Pro His Leu Tyr Thr Leu Phe His Gln Ala His Val Ala Gly 
705                 710                 715                 720 


Glu Thr Val Ala Arg Pro Leu Phe Leu Glu Phe Pro Lys Asp Ser Ser 
                725                 730                 735     


Thr Trp Thr Val Asp His Gln Leu Leu Trp Gly Glu Ala Leu Leu Ile 
            740                 745                 750         


Thr Pro Val Leu Gln Ala Gly Lys Ala Glu Val Thr Gly Tyr Phe Pro 
        755                 760                 765             


Leu Gly Thr Trp Tyr Asp Leu Gln Thr Val Pro Ile Glu Ala Leu Gly 
    770                 775                 780                 


Ser Leu Pro Pro Pro Pro Ala Ala Pro Arg Glu Pro Ala Ile His Ser 
785                 790                 795                 800 


Glu Gly Gln Trp Val Thr Leu Pro Ala Pro Leu Asp Thr Ile Asn Val 
                805                 810                 815     


His Leu Arg Ala Gly Tyr Ile Ile Pro Leu Gln Gly Pro Gly Leu Thr 
            820                 825                 830         


Thr Thr Glu Ser Arg Gln Gln Pro Met Ala Leu Ala Val Ala Leu Thr 
        835                 840                 845             


Lys Gly Gly Glu Ala Arg Gly Glu Leu Phe Trp Asp Asp Gly Glu Ser 
    850                 855                 860                 


Leu Glu Val Leu Glu Arg Gly Ala Tyr Thr Gln Val Ile Phe Leu Ala 
865                 870                 875                 880 


Arg Asn Asn Thr Ile Val Asn Glu Leu Val Arg Val Thr Ser Glu Gly 
                885                 890                 895     


Ala Gly Leu Gln Leu Gln Lys Val Thr Val Leu Gly Val Ala Thr Ala 
            900                 905                 910         


Pro Gln Gln Val Leu Ser Asn Gly Val Pro Val Ser Asn Phe Thr Tyr 
        915                 920                 925             


Ser Pro Asp Thr Lys Val Leu Asp Ile Cys Val Ser Leu Leu Met Gly 
    930                 935                 940                 


Glu Gln Phe Leu Val Ser Trp Cys 
945                 950         


<210>  4
<211>  2856
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Engineered hGAAI Coding sequence

<400>  4
atgggcgtta gacaccctcc ttgctctcac agactgctgg ccgtgtgtgc tctggtgtct       60

ctggctacag ctgccctgct gggacatatc ctgctgcacg actttctgct ggtgcccaga      120

gagctgtctg gcagctctcc tgtgctggaa gagacacacc ctgcacatca gcagggcgcc      180

tctagacctg gacctagaga cgctcaggcc catcctggca gacctagagc tgtgcccaca      240

cagtgtgacg tgccacctaa cagcagattc gactgcgccc ctgacaaggc catcacacaa      300

gagcagtgtg aagccagagg ctgctgctac atccctgcca aacaaggact gcagggcgct      360

cagatgggac agccctggtg cttcttccca ccatcttacc ccagctacaa gctggaaaac      420

ctgagcagca gcgagatggg ctacaccgcc acactgacca gaaccacacc tacattcttc      480

ccgaaggaca tcctgacact gcggctggac gtgatgatgg aaaccgagaa ccggctgcac      540

ttcaccatca aggaccccgc caatcggaga tacgaggtgc cactggaaac ccctcacgtg      600

cactctagag ccccatctcc actgtacagc gtggaatttt ccgaggaacc cttcggcgtg      660

atcgtgcgga gacagctgga tggaagagtg ctgctgaaca ccacagtggc ccctctgttc      720

ttcgccgacc agtttctgca gctgagcacc agcctgccta gccagtatat cacaggcctg      780

gccgagcacc tgtctccact gatgctgagc acatcctgga ccagaatcac cctgtggaac      840

agagatctgg cccctacacc tggcgccaac ctgtacggct ctcacccctt ttatctggcc      900

ctggaagacg gcggatctgc ccacggtgtt tttctgctga actccaacgc catggacgtg      960

gtgctgcagc catctcctgc tctgtcttgg agaagcacag gcggcatcct ggacgtgtac     1020

atctttctgg gccccgagcc taagagcgtg gtgcagcagt atctggacgt cgtgggctac     1080

cccttcatgc ctccttattg gggcctgggc ttccacctgt gcaggtgggg atacagcagc     1140

accgccatca ccagacaggt ggtggaaaac atgacccggg ctcacttccc actggacgtg     1200

cagtggaacg acctggacta catggacagc agacgggact tcaccttcaa caaggacggc     1260

ttcagagact tccccgccat ggtgcaagaa ctgcaccaag gcggcagacg gtacatgatg     1320

attgtggacc cagccatcag ctctagcggc cctgccggaa gctacagacc ttacgatgag     1380

ggcctgagaa gaggcgtgtt catcaccaac gagacaggcc agcctctgat cggcaaagtg     1440

tggcctggca gcacagcctt tccagacttc acaaacccca ccgctctggc ttggtgggaa     1500

gatatggtgg ccgagttcca cgatcaggtg cccttcgatg gcatgtggat cgacatgaac     1560

gagcccagca acttcatccg gggcagcgag gacggctgcc ccaacaacga actggaaaat     1620

cctccttacg tgcccggcgt tgtcggcgga acacttcagg ccgctacaat ctgtgccagc     1680

agccaccagt tcctcagcac ccactacaac ctgcacaacc tgtacggcct gaccgaggcc     1740

attgcctctc atagagccct ggttaaggcc agaggcaccc ggccttttgt gatcagcaga     1800

agcacattcg ccggccacgg cagatacgcc ggacattgga caggcgacgt gtggtctagt     1860

tgggagcagc tggctagcag cgtgccagag atcctgcagt tcaatctgct gggcgtgcca     1920

ctcgtgggag ccgacgtttg tggcttcctg ggcaacacca gcgaggaact gtgtgtgcgt     1980

tggacacagc tgggcgcctt ctatcccttc atgagaaacc acaacagcct gctgagcctg     2040

cctcaagagc cctacagctt tagcgagcct gcacagcagg ccatgagaaa ggccctgact     2100

ctgagatacg ctctgctgcc ccacctgtac accctgtttc accaggctca cgtggccggg     2160

gagacagtgg ctagacctct gttcctggaa tttcccaagg acagctccac ctggaccgtg     2220

gatcatcagc tgctgtgggg agaagccctg ctcatcacac ctgttctgca ggccggaaag     2280

gccgaagtga ccggctattt tcctctcggc acttggtacg acctgcagac cgtgcctatt     2340

gaggccctgg gatctcttcc tccacctcct gctgctccta gagagcctgc catccactct     2400

gaaggccagt gggttacact gcccgctcct ctggacacca tcaacgtgca cctgagagct     2460

ggctacatca tcccactgca aggccctggc ctgaccacaa ccgaatctag acagcagccc     2520

atggctctgg ccgtggctct tacaaaaggc ggagaggcta gaggcgagct gttctgggac     2580

gacggcgagt ctctggaagt gctggaacgg ggcgcttata cccaagtgat cttcctggcc     2640

agaaacaaca ccatcgtgaa cgaactcgtg cgcgtgacca gtgaaggtgc tggactgcaa     2700

ctgcagaaag tgaccgtgct cggagtggcc acagctcctc aacaggtgct gtctaacggc     2760

gtgcccgtgt ccaacttcac atacagcccc gacaccaagg tcctggacat ctgtgtgtca     2820

ctgctgatgg gcgagcagtt cctggtgtcc tggtgc                               2856


<210>  5
<211>  2859
<212>  DNA
<213>  Homo sapiens

<400>  5
atgggagtga ggcacccgcc ctgctcccac cggctcctgg ccgtctgcgc cctcgtgtcc       60

ttggcaaccg ctgcactcct ggggcacatc ctactccatg atttcctgct ggttccccga      120

gagctgagtg gctcctcccc agtcctggag gagactcacc cagctcacca gcagggagcc      180

agtagaccag ggccccggga tgcccaggca caccccggcc gtcccagagc agtgcccaca      240

cagtgcgacg tcccccccaa cagccgcttc gattgcgccc ctgacaaggc catcacccag      300

gaacagtgcg aggcccgcgg ctgttgctac atccctgcaa agcaggggct gcagggagcc      360

cagatggggc agccctggtg cttcttccca cccagctacc ccagctacaa gctggagaac      420

ctgagctcct ctgaaatggg ctacacggcc accctgaccc gtaccacccc caccttcttc      480

cccaaggaca tcctgaccct gcggctggac gtgatgatgg agactgagaa ccgcctccac      540

ttcacgatca aagatccagc taacaggcgc tacgaggtgc ccttggagac cccgcatgtc      600

cacagccggg caccgtcccc actctacagc gtggagttct ccgaggagcc cttcggggtg      660

atcgtgcgcc ggcagctgga cggccgcgtg ctgctgaaca cgacggtggc gcccctgttc      720

tttgcggacc agttccttca gctgtccacc tcgctgccct cgcagtatat cacaggcctc      780

gccgagcacc tcagtcccct gatgctcagc accagctgga ccaggatcac cctgtggaac      840

cgggaccttg cgcccacgcc cggtgcgaac ctctacgggt ctcacccttt ctacctggcg      900

ctggaggacg gcgggtcggc acacggggtg ttcctgctaa acagcaatgc catggatgtg      960

gtcctgcagc cgagccctgc ccttagctgg aggtcgacag gtgggatcct ggatgtctac     1020

atcttcctgg gcccagagcc caagagcgtg gtgcagcagt acctggacgt tgtgggatac     1080

ccgttcatgc cgccatactg gggcctgggc ttccacctgt gccgctgggg ctactcctcc     1140

accgctatca cccgccaggt ggtggagaac atgaccaggg cccacttccc cctggacgtc     1200

cagtggaacg acctggacta catggactcc cggagggact tcacgttcaa caaggatggc     1260

ttccgggact tcccggccat ggtgcaggag ctgcaccagg gcggccggcg ctacatgatg     1320

atcgtggatc ctgccatcag cagctcgggc cctgccggga gctacaggcc ctacgacgag     1380

ggtctgcgga ggggggtttt catcaccaac gagaccggcc agccgctgat tgggaaggta     1440

tggcccgggt ccactgcctt ccccgacttc accaacccca cagccctggc ctggtgggag     1500

gacatggtgg ctgagttcca tgaccaggtg cccttcgacg gcatgtggat tgacatgaac     1560

gagccttcca acttcatcag gggctctgag gacggctgcc ccaacaatga gctggagaac     1620

ccaccctacg tgcctggggt ggttgggggg accctccagg cggccaccat ctgtgcctcc     1680

agccaccagt ttctctccac acactacaac ctgcacaacc tctacggcct gaccgaagcc     1740

atcgcctccc acagggcgct ggtgaaggct cgggggacac gcccatttgt gatctcccgc     1800

tcgacctttg ctggccacgg ccgatacgcc ggccactgga cgggggacgt gtggagctcc     1860

tgggagcagc tcgcctcctc cgtgccagaa atcctgcagt ttaacctgct gggggtgcct     1920

ctggtcgggg ccgacgtctg cggcttcctg ggcaacacct cagaggagct gtgtgtgcgc     1980

tggacccagc tgggggcctt ctaccccttc atgcggaacc acaacagcct gctcagtctg     2040

ccccaggagc cgtacagctt cagcgagccg gcccagcagg ccatgaggaa ggccctcacc     2100

ctgcgctacg cactcctccc ccacctctac acactgttcc accaggccca cgtcgcgggg     2160

gagaccgtgg cccggcccct cttcctggag ttccccaagg actctagcac ctggactgtg     2220

gaccaccagc tcctgtgggg ggaggccctg ctcatcaccc cagtgctcca ggccgggaag     2280

gccgaagtga ctggctactt ccccttgggc acatggtacg acctgcagac ggtgccaata     2340

gaggcccttg gcagcctccc acccccacct gcagctcccc gtgagccagc catccacagc     2400

gaggggcagt gggtgacgct gccggccccc ctggacacca tcaacgtcca cctccgggct     2460

gggtacatca tccccctgca gggccctggc ctcacaacca cagagtcccg ccagcagccc     2520

atggccctgg ctgtggccct gaccaagggt ggggaggccc gaggggagct tttctgggac     2580

gatggagaga gcctggaagt gctggagcga ggggcctaca cacaggtcat cttcctggcc     2640

aggaataaca cgatcgtgaa tgagctggta cgtgtgacca gtgagggagc tggcctgcag     2700

ctgcagaagg tgactgtcct gggcgtggcc acggcgcccc agcaggtcct ctccaacggt     2760

gtccctgtct ccaacttcac ctacagcccc gacaccaagg tcctggacat ctgtgtctcg     2820

ctgttgatgg gagagcagtt tctcgtcagc tggtgttag                            2859


<210>  6
<211>  982
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Fusion Protein comprising hGAA780I

<400>  6

Met Lys Leu Ser Leu Val Ala Ala Met Leu Leu Leu Leu Ser Ala Ala 
1               5                   10                  15      


Arg Ala Ser Arg Thr Leu Cys Gly Gly Glu Leu Val Asp Thr Leu Gln 
            20                  25                  30          


Phe Val Cys Gly Asp Arg Gly Phe Leu Phe Ser Arg Pro Ala Ser Arg 
        35                  40                  45              


Val Ser Arg Arg Ser Arg Gly Ile Val Glu Glu Cys Cys Phe Arg Ser 
    50                  55                  60                  


Cys Asp Leu Ala Leu Leu Glu Thr Tyr Cys Ala Thr Pro Ala Arg Ser 
65                  70                  75                  80  


Glu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Arg Pro Gly Pro Arg 
                85                  90                  95      


Asp Ala Gln Ala His Pro Gly Arg Pro Arg Ala Val Pro Thr Gln Cys 
            100                 105                 110         


Asp Val Pro Pro Asn Ser Arg Phe Asp Cys Ala Pro Asp Lys Ala Ile 
        115                 120                 125             


Thr Gln Glu Gln Cys Glu Ala Arg Gly Cys Cys Tyr Ile Pro Ala Lys 
    130                 135                 140                 


Gln Gly Leu Gln Gly Ala Gln Met Gly Gln Pro Trp Cys Phe Phe Pro 
145                 150                 155                 160 


Pro Ser Tyr Pro Ser Tyr Lys Leu Glu Asn Leu Ser Ser Ser Glu Met 
                165                 170                 175     


Gly Tyr Thr Ala Thr Leu Thr Arg Thr Thr Pro Thr Phe Phe Pro Lys 
            180                 185                 190         


Asp Ile Leu Thr Leu Arg Leu Asp Val Met Met Glu Thr Glu Asn Arg 
        195                 200                 205             


Leu His Phe Thr Ile Lys Asp Pro Ala Asn Arg Arg Tyr Glu Val Pro 
    210                 215                 220                 


Leu Glu Thr Pro His Val His Ser Arg Ala Pro Ser Pro Leu Tyr Ser 
225                 230                 235                 240 


Val Glu Phe Ser Glu Glu Pro Phe Gly Val Ile Val Arg Arg Gln Leu 
                245                 250                 255     


Asp Gly Arg Val Leu Leu Asn Thr Thr Val Ala Pro Leu Phe Phe Ala 
            260                 265                 270         


Asp Gln Phe Leu Gln Leu Ser Thr Ser Leu Pro Ser Gln Tyr Ile Thr 
        275                 280                 285             


Gly Leu Ala Glu His Leu Ser Pro Leu Met Leu Ser Thr Ser Trp Thr 
    290                 295                 300                 


Arg Ile Thr Leu Trp Asn Arg Asp Leu Ala Pro Thr Pro Gly Ala Asn 
305                 310                 315                 320 


Leu Tyr Gly Ser His Pro Phe Tyr Leu Ala Leu Glu Asp Gly Gly Ser 
                325                 330                 335     


Ala His Gly Val Phe Leu Leu Asn Ser Asn Ala Met Asp Val Val Leu 
            340                 345                 350         


Gln Pro Ser Pro Ala Leu Ser Trp Arg Ser Thr Gly Gly Ile Leu Asp 
        355                 360                 365             


Val Tyr Ile Phe Leu Gly Pro Glu Pro Lys Ser Val Val Gln Gln Tyr 
    370                 375                 380                 


Leu Asp Val Val Gly Tyr Pro Phe Met Pro Pro Tyr Trp Gly Leu Gly 
385                 390                 395                 400 


Phe His Leu Cys Arg Trp Gly Tyr Ser Ser Thr Ala Ile Thr Arg Gln 
                405                 410                 415     


Val Val Glu Asn Met Thr Arg Ala His Phe Pro Leu Asp Val Gln Trp 
            420                 425                 430         


Asn Asp Leu Asp Tyr Met Asp Ser Arg Arg Asp Phe Thr Phe Asn Lys 
        435                 440                 445             


Asp Gly Phe Arg Asp Phe Pro Ala Met Val Gln Glu Leu His Gln Gly 
    450                 455                 460                 


Gly Arg Arg Tyr Met Met Ile Val Asp Pro Ala Ile Ser Ser Ser Gly 
465                 470                 475                 480 


Pro Ala Gly Ser Tyr Arg Pro Tyr Asp Glu Gly Leu Arg Arg Gly Val 
                485                 490                 495     


Phe Ile Thr Asn Glu Thr Gly Gln Pro Leu Ile Gly Lys Val Trp Pro 
            500                 505                 510         


Gly Ser Thr Ala Phe Pro Asp Phe Thr Asn Pro Thr Ala Leu Ala Trp 
        515                 520                 525             


Trp Glu Asp Met Val Ala Glu Phe His Asp Gln Val Pro Phe Asp Gly 
    530                 535                 540                 


Met Trp Ile Asp Met Asn Glu Pro Ser Asn Phe Ile Arg Gly Ser Glu 
545                 550                 555                 560 


Asp Gly Cys Pro Asn Asn Glu Leu Glu Asn Pro Pro Tyr Val Pro Gly 
                565                 570                 575     


Val Val Gly Gly Thr Leu Gln Ala Ala Thr Ile Cys Ala Ser Ser His 
            580                 585                 590         


Gln Phe Leu Ser Thr His Tyr Asn Leu His Asn Leu Tyr Gly Leu Thr 
        595                 600                 605             


Glu Ala Ile Ala Ser His Arg Ala Leu Val Lys Ala Arg Gly Thr Arg 
    610                 615                 620                 


Pro Phe Val Ile Ser Arg Ser Thr Phe Ala Gly His Gly Arg Tyr Ala 
625                 630                 635                 640 


Gly His Trp Thr Gly Asp Val Trp Ser Ser Trp Glu Gln Leu Ala Ser 
                645                 650                 655     


Ser Val Pro Glu Ile Leu Gln Phe Asn Leu Leu Gly Val Pro Leu Val 
            660                 665                 670         


Gly Ala Asp Val Cys Gly Phe Leu Gly Asn Thr Ser Glu Glu Leu Cys 
        675                 680                 685             


Val Arg Trp Thr Gln Leu Gly Ala Phe Tyr Pro Phe Met Arg Asn His 
    690                 695                 700                 


Asn Ser Leu Leu Ser Leu Pro Gln Glu Pro Tyr Ser Phe Ser Glu Pro 
705                 710                 715                 720 


Ala Gln Gln Ala Met Arg Lys Ala Leu Thr Leu Arg Tyr Ala Leu Leu 
                725                 730                 735     


Pro His Leu Tyr Thr Leu Phe His Gln Ala His Val Ala Gly Glu Thr 
            740                 745                 750         


Val Ala Arg Pro Leu Phe Leu Glu Phe Pro Lys Asp Ser Ser Thr Trp 
        755                 760                 765             


Thr Val Asp His Gln Leu Leu Trp Gly Glu Ala Leu Leu Ile Thr Pro 
    770                 775                 780                 


Val Leu Gln Ala Gly Lys Ala Glu Val Thr Gly Tyr Phe Pro Leu Gly 
785                 790                 795                 800 


Thr Trp Tyr Asp Leu Gln Thr Val Pro Ile Glu Ala Leu Gly Ser Leu 
                805                 810                 815     


Pro Pro Pro Pro Ala Ala Pro Arg Glu Pro Ala Ile His Ser Glu Gly 
            820                 825                 830         


Gln Trp Val Thr Leu Pro Ala Pro Leu Asp Thr Ile Asn Val His Leu 
        835                 840                 845             


Arg Ala Gly Tyr Ile Ile Pro Leu Gln Gly Pro Gly Leu Thr Thr Thr 
    850                 855                 860                 


Glu Ser Arg Gln Gln Pro Met Ala Leu Ala Val Ala Leu Thr Lys Gly 
865                 870                 875                 880 


Gly Glu Ala Arg Gly Glu Leu Phe Trp Asp Asp Gly Glu Ser Leu Glu 
                885                 890                 895     


Val Leu Glu Arg Gly Ala Tyr Thr Gln Val Ile Phe Leu Ala Arg Asn 
            900                 905                 910         


Asn Thr Ile Val Asn Glu Leu Val Arg Val Thr Ser Glu Gly Ala Gly 
        915                 920                 925             


Leu Gln Leu Gln Lys Val Thr Val Leu Gly Val Ala Thr Ala Pro Gln 
    930                 935                 940                 


Gln Val Leu Ser Asn Gly Val Pro Val Ser Asn Phe Thr Tyr Ser Pro 
945                 950                 955                 960 


Asp Thr Lys Val Leu Asp Ile Cys Val Ser Leu Leu Met Gly Glu Gln 
                965                 970                 975     


Phe Leu Val Ser Trp Cys 
            980         


<210>  7
<211>  2952
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Engineered sequence encoding fusion protein comprising GAAV780I


<220>
<221>  misc_feature
<222>  (810)..(810)
<223>  V810I

<400>  7
atgaagctgt ctctggtggc tgctatgctg ctgctcctgt ctgccgccag agccagcaga       60

acactttgtg gcggagagct ggtggacacc ctgcagtttg tgtgtggcga cagaggcttc      120

ctgttcagca gacctgccag ccgggtttcc agacggtcta gaggaatcgt ggaagagtgc      180

tgcttcagaa gctgcgatct ggccctgctg gaaacctact gtgccacacc agccagatct      240

gaaggcggcg gaggatctgg cggaggcgga tctagacctg gacctagaga cgcccaggct      300

caccctggta gacctagagc tgtgcctaca cagtgcgacg tgccacctaa cagcagattc      360

gactgcgccc ctgacaaggc catcacacaa gagcagtgtg aagccagagg ctgctgctac      420

atccctgcca aacaaggact gcagggcgcc cagatgggac agccttggtg cttcttccca      480

ccatcttacc ccagctacaa gctggaaaac ctgagcagct ccgagatggg ctacaccgcc      540

acactgacca gaaccacacc tacattcttc ccgaaggaca tcctgacact gcggctggac      600

gtgatgatgg aaaccgagaa ccggctgcac ttcaccatca aggaccccgc caatcggaga      660

tacgaggtgc ccctggaaac accccacgtg cactctagag caccctctcc actgtacagc      720

gtggaatttt ccgaggaacc cttcggcgtg atcgtgcgga gacagctgga tggcagagtg      780

ctcctgaata ccacagtggc ccctctgttc ttcgccgacc agtttctgca gctgagcacc      840

agcctgccta gccagtatat cacaggcctg gccgagcatc tgagccctct gatgctgagc      900

acatcctgga ccagaatcac cctgtggaac cgcgacctgg ctcctacacc tggcgccaat      960

ctgtacggct ctcacccctt ctacctggca ctggaagacg gtggatctgc ccacggtgtc     1020

tttctgctga atagcaacgc catggacgtg gtgctgcagc cctctcctgc actgtcttgg     1080

agatctacag gcggcatcct ggacgtgtac atctttctgg gccccgagcc taagagcgtg     1140

gtgcagcagt atctggacgt cgtgggctac cccttcatgc ctccttattg gggcctgggc     1200

ttccacctgt gtaggtgggg ctacagcagc accgccatca ccagacaggt ggtggaaaac     1260

atgacccggg ctcacttccc actggacgtg cagtggaacg acctggacta catggacagc     1320

agacgggact tcaccttcaa caaggacggc ttcagagact tccccgccat ggtgcaagag     1380

ctgcatcaag gcggacggcg gtacatgatg attgtggacc ctgccatcag cagctctgga     1440

ccagccggca gctacagacc ttacgatgag ggactgagaa gaggcgtgtt catcaccaac     1500

gagacaggcc agcctctgat cggcaaagtg tggcctggca gcacagcctt tccagacttc     1560

acaaacccca ccgctctggc ttggtgggaa gatatggtgg ccgagttcca cgatcaggtg     1620

cccttcgatg gcatgtggat cgacatgaac gagcccagca acttcatccg gggcagcgag     1680

gatggctgcc ccaacaacga actagaaaat cctccttacg tgcccggcgt tgtcggcgga     1740

acacttcagg ccgctacaat ctgtgccagc agccatcagt ttctgagcac ccactacaac     1800

ctgcacaacc tgtacggcct gaccgaggcc attgcctctc atagagccct ggttaaggcc     1860

agaggcaccc ggccttttgt gatcagcaga agcacattcg ccggccacgg cagatacgca     1920

ggacattgga caggcgacgt gtggtctagt tgggagcagc tggctagcag cgtgccagag     1980

atcctgcagt tcaatctgct gggcgtgcca ctcgtgggag ccgacgtttg tggcttcctg     2040

ggcaacacca gcgaggaact gtgtgtgcgt tggacacagc tgggcgcctt ctatcccttc     2100

atgagaaacc acaacagcct gctgagcctg cctcaagagc cctacagctt tagcgagcct     2160

gcacagcagg ccatgagaaa ggccctgact ctgagatacg ccctgctgcc tcacctgtac     2220

accctgtttc atcaggccca cgtggcaggc gagacagtgg ctagacctct gttcctggaa     2280

tttcccaagg acagctccac ctggaccgtg gatcatcagc tgctgtgggg agaagccctg     2340

ctgattacac cagtgctgca ggccggaaag gccgaagtga caggctattt ccctctcggc     2400

acttggtacg acctgcagac cgtgcctatc gaggctctgg gatctcttcc tccacctcct     2460

gccgctccta gagagcctgc cattcactct gaaggccagt gggttaccct gcctgctcct     2520

ctggacacca tcaacgtgca cctgagagcc ggctacatca tccctctgca aggccctggc     2580

ctgaccacaa ccgaatctag acagcagccc atggcactgg ccgtggctct tacaaaaggc     2640

ggagaggcta gaggcgagct gttctgggat gatggcgaga gcctagaagt gctggaacgg     2700

ggcgcttata cccaagtgat cttcctggcc agaaacaaca ccatcgtgaa cgaactcgtg     2760

cgcgtgacca gtgaaggtgc tggactgcaa ctgcagaaag tgaccgtgct cggagtggcc     2820

acagctcctc agcaggttct gtctaatggc gtgcccgtgt ccaacttcac atacagcccc     2880

gacaccaagg tcctggacat ctgtgtgtcc ctgcttatgg gcgagcagtt cctggtgtcc     2940

tggtgctgat aa                                                         2952


<210>  8
<211>  934
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CAG promoter


<220>
<221>  misc_feature
<222>  (1)..(243)
<223>  CMV early enhancer element

<220>
<221>  misc_feature
<222>  (244)..(525)
<223>  Chicken Beta actin promoter

<220>
<221>  misc_feature
<222>  (526)..(934)
<223>  hybrid intron in CAG (b-actin intron and rabbit beta 1-globin

<220>
<221>  misc_feature
<222>  (526)..(934)
<223>  hybrid intron

<400>  8
attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg       60

tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat      120

gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca      180

gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat      240

taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc      300

acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg      360

gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg      420

gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag      480

gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg      540

ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact      600

gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta      660

gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct      720

ccgggagggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg gacggctgcc      780

ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag      840

cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg      900

ttattgtgct gtctcatcat tttggcaaag aatt                                  934


<210>  9
<211>  127
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Rabbit globin polyA

<400>  9
gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact       60

tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc      120

tcactcg                                                                127


<210>  10
<211>  188
<212>  DNA
<213>  adeno-associated virus 2

<400>  10
gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt       60

tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac      120

taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc      180

taggaaga                                                               188


<210>  11
<211>  188
<212>  DNA
<213>  adeno-associated virus 2

<400>  11
tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg       60

aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg      120

ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag      180

cgcgcagc                                                               188


<210>  12
<211>  81
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Engineered hGAAV780I signal peptide


<220>
<221>  sig_peptide
<222>  (1)..(81)

<220>
<221>  CDS
<222>  (1)..(81)

<400>  12
atg ggc gtt aga cac cct cct tgc tct cac aga ctg ctg gcc gtg tgt         48
Met Gly Val Arg His Pro Pro Cys Ser His Arg Leu Leu Ala Val Cys           
1               5                   10                  15                

gct ctg gtg tct ctg gct aca gct gcc ctg ctg                             81
Ala Leu Val Ser Leu Ala Thr Ala Ala Leu Leu                               
            20                  25                                        


<210>  13
<211>  27
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  13

Met Gly Val Arg His Pro Pro Cys Ser His Arg Leu Leu Ala Val Cys 
1               5                   10                  15      


Ala Leu Val Ser Leu Ala Thr Ala Ala Leu Leu 
            20                  25          


<210>  14
<211>  2649
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  engineered hGAAV780I mature protein


<220>
<221>  CDS
<222>  (1)..(2649)

<400>  14
gcc cat cct ggc aga cct aga gct gtg ccc aca cag tgt gac gtg cca         48
Ala His Pro Gly Arg Pro Arg Ala Val Pro Thr Gln Cys Asp Val Pro           
1               5                   10                  15                

cct aac agc aga ttc gac tgc gcc cct gac aag gcc atc aca caa gag         96
Pro Asn Ser Arg Phe Asp Cys Ala Pro Asp Lys Ala Ile Thr Gln Glu           
            20                  25                  30                    

cag tgt gaa gcc aga ggc tgc tgc tac atc cct gcc aaa caa gga ctg        144
Gln Cys Glu Ala Arg Gly Cys Cys Tyr Ile Pro Ala Lys Gln Gly Leu           
        35                  40                  45                        

cag ggc gct cag atg gga cag ccc tgg tgc ttc ttc cca cca tct tac        192
Gln Gly Ala Gln Met Gly Gln Pro Trp Cys Phe Phe Pro Pro Ser Tyr           
    50                  55                  60                            

ccc agc tac aag ctg gaa aac ctg agc agc agc gag atg ggc tac acc        240
Pro Ser Tyr Lys Leu Glu Asn Leu Ser Ser Ser Glu Met Gly Tyr Thr           
65                  70                  75                  80            

gcc aca ctg acc aga acc aca cct aca ttc ttc ccg aag gac atc ctg        288
Ala Thr Leu Thr Arg Thr Thr Pro Thr Phe Phe Pro Lys Asp Ile Leu           
                85                  90                  95                

aca ctg cgg ctg gac gtg atg atg gaa acc gag aac cgg ctg cac ttc        336
Thr Leu Arg Leu Asp Val Met Met Glu Thr Glu Asn Arg Leu His Phe           
            100                 105                 110                   

acc atc aag gac ccc gcc aat cgg aga tac gag gtg cca ctg gaa acc        384
Thr Ile Lys Asp Pro Ala Asn Arg Arg Tyr Glu Val Pro Leu Glu Thr           
        115                 120                 125                       

cct cac gtg cac tct aga gcc cca tct cca ctg tac agc gtg gaa ttt        432
Pro His Val His Ser Arg Ala Pro Ser Pro Leu Tyr Ser Val Glu Phe           
    130                 135                 140                           

tcc gag gaa ccc ttc ggc gtg atc gtg cgg aga cag ctg gat gga aga        480
Ser Glu Glu Pro Phe Gly Val Ile Val Arg Arg Gln Leu Asp Gly Arg           
145                 150                 155                 160           

gtg ctg ctg aac acc aca gtg gcc cct ctg ttc ttc gcc gac cag ttt        528
Val Leu Leu Asn Thr Thr Val Ala Pro Leu Phe Phe Ala Asp Gln Phe           
                165                 170                 175               

ctg cag ctg agc acc agc ctg cct agc cag tat atc aca ggc ctg gcc        576
Leu Gln Leu Ser Thr Ser Leu Pro Ser Gln Tyr Ile Thr Gly Leu Ala           
            180                 185                 190                   

gag cac ctg tct cca ctg atg ctg agc aca tcc tgg acc aga atc acc        624
Glu His Leu Ser Pro Leu Met Leu Ser Thr Ser Trp Thr Arg Ile Thr           
        195                 200                 205                       

ctg tgg aac aga gat ctg gcc cct aca cct ggc gcc aac ctg tac ggc        672
Leu Trp Asn Arg Asp Leu Ala Pro Thr Pro Gly Ala Asn Leu Tyr Gly           
    210                 215                 220                           

tct cac ccc ttt tat ctg gcc ctg gaa gac ggc gga tct gcc cac ggt        720
Ser His Pro Phe Tyr Leu Ala Leu Glu Asp Gly Gly Ser Ala His Gly           
225                 230                 235                 240           

gtt ttt ctg ctg aac tcc aac gcc atg gac gtg gtg ctg cag cca tct        768
Val Phe Leu Leu Asn Ser Asn Ala Met Asp Val Val Leu Gln Pro Ser           
                245                 250                 255               

cct gct ctg tct tgg aga agc aca ggc ggc atc ctg gac gtg tac atc        816
Pro Ala Leu Ser Trp Arg Ser Thr Gly Gly Ile Leu Asp Val Tyr Ile           
            260                 265                 270                   

ttt ctg ggc ccc gag cct aag agc gtg gtg cag cag tat ctg gac gtc        864
Phe Leu Gly Pro Glu Pro Lys Ser Val Val Gln Gln Tyr Leu Asp Val           
        275                 280                 285                       

gtg ggc tac ccc ttc atg cct cct tat tgg ggc ctg ggc ttc cac ctg        912
Val Gly Tyr Pro Phe Met Pro Pro Tyr Trp Gly Leu Gly Phe His Leu           
    290                 295                 300                           

tgc agg tgg gga tac agc agc acc gcc atc acc aga cag gtg gtg gaa        960
Cys Arg Trp Gly Tyr Ser Ser Thr Ala Ile Thr Arg Gln Val Val Glu           
305                 310                 315                 320           

aac atg acc cgg gct cac ttc cca ctg gac gtg cag tgg aac gac ctg       1008
Asn Met Thr Arg Ala His Phe Pro Leu Asp Val Gln Trp Asn Asp Leu           
                325                 330                 335               

gac tac atg gac agc aga cgg gac ttc acc ttc aac aag gac ggc ttc       1056
Asp Tyr Met Asp Ser Arg Arg Asp Phe Thr Phe Asn Lys Asp Gly Phe           
            340                 345                 350                   

aga gac ttc ccc gcc atg gtg caa gaa ctg cac caa ggc ggc aga cgg       1104
Arg Asp Phe Pro Ala Met Val Gln Glu Leu His Gln Gly Gly Arg Arg           
        355                 360                 365                       

tac atg atg att gtg gac cca gcc atc agc tct agc ggc cct gcc gga       1152
Tyr Met Met Ile Val Asp Pro Ala Ile Ser Ser Ser Gly Pro Ala Gly           
    370                 375                 380                           

agc tac aga cct tac gat gag ggc ctg aga aga ggc gtg ttc atc acc       1200
Ser Tyr Arg Pro Tyr Asp Glu Gly Leu Arg Arg Gly Val Phe Ile Thr           
385                 390                 395                 400           

aac gag aca ggc cag cct ctg atc ggc aaa gtg tgg cct ggc agc aca       1248
Asn Glu Thr Gly Gln Pro Leu Ile Gly Lys Val Trp Pro Gly Ser Thr           
                405                 410                 415               

gcc ttt cca gac ttc aca aac ccc acc gct ctg gct tgg tgg gaa gat       1296
Ala Phe Pro Asp Phe Thr Asn Pro Thr Ala Leu Ala Trp Trp Glu Asp           
            420                 425                 430                   

atg gtg gcc gag ttc cac gat cag gtg ccc ttc gat ggc atg tgg atc       1344
Met Val Ala Glu Phe His Asp Gln Val Pro Phe Asp Gly Met Trp Ile           
        435                 440                 445                       

gac atg aac gag ccc agc aac ttc atc cgg ggc agc gag gac ggc tgc       1392
Asp Met Asn Glu Pro Ser Asn Phe Ile Arg Gly Ser Glu Asp Gly Cys           
    450                 455                 460                           

ccc aac aac gaa ctg gaa aat cct cct tac gtg ccc ggc gtt gtc ggc       1440
Pro Asn Asn Glu Leu Glu Asn Pro Pro Tyr Val Pro Gly Val Val Gly           
465                 470                 475                 480           

gga aca ctt cag gcc gct aca atc tgt gcc agc agc cac cag ttc ctc       1488
Gly Thr Leu Gln Ala Ala Thr Ile Cys Ala Ser Ser His Gln Phe Leu           
                485                 490                 495               

agc acc cac tac aac ctg cac aac ctg tac ggc ctg acc gag gcc att       1536
Ser Thr His Tyr Asn Leu His Asn Leu Tyr Gly Leu Thr Glu Ala Ile           
            500                 505                 510                   

gcc tct cat aga gcc ctg gtt aag gcc aga ggc acc cgg cct ttt gtg       1584
Ala Ser His Arg Ala Leu Val Lys Ala Arg Gly Thr Arg Pro Phe Val           
        515                 520                 525                       

atc agc aga agc aca ttc gcc ggc cac ggc aga tac gcc gga cat tgg       1632
Ile Ser Arg Ser Thr Phe Ala Gly His Gly Arg Tyr Ala Gly His Trp           
    530                 535                 540                           

aca ggc gac gtg tgg tct agt tgg gag cag ctg gct agc agc gtg cca       1680
Thr Gly Asp Val Trp Ser Ser Trp Glu Gln Leu Ala Ser Ser Val Pro           
545                 550                 555                 560           

gag atc ctg cag ttc aat ctg ctg ggc gtg cca ctc gtg gga gcc gac       1728
Glu Ile Leu Gln Phe Asn Leu Leu Gly Val Pro Leu Val Gly Ala Asp           
                565                 570                 575               

gtt tgt ggc ttc ctg ggc aac acc agc gag gaa ctg tgt gtg cgt tgg       1776
Val Cys Gly Phe Leu Gly Asn Thr Ser Glu Glu Leu Cys Val Arg Trp           
            580                 585                 590                   

aca cag ctg ggc gcc ttc tat ccc ttc atg aga aac cac aac agc ctg       1824
Thr Gln Leu Gly Ala Phe Tyr Pro Phe Met Arg Asn His Asn Ser Leu           
        595                 600                 605                       

ctg agc ctg cct caa gag ccc tac agc ttt agc gag cct gca cag cag       1872
Leu Ser Leu Pro Gln Glu Pro Tyr Ser Phe Ser Glu Pro Ala Gln Gln           
    610                 615                 620                           

gcc atg aga aag gcc ctg act ctg aga tac gct ctg ctg ccc cac ctg       1920
Ala Met Arg Lys Ala Leu Thr Leu Arg Tyr Ala Leu Leu Pro His Leu           
625                 630                 635                 640           

tac acc ctg ttt cac cag gct cac gtg gcc ggg gag aca gtg gct aga       1968
Tyr Thr Leu Phe His Gln Ala His Val Ala Gly Glu Thr Val Ala Arg           
                645                 650                 655               

cct ctg ttc ctg gaa ttt ccc aag gac agc tcc acc tgg acc gtg gat       2016
Pro Leu Phe Leu Glu Phe Pro Lys Asp Ser Ser Thr Trp Thr Val Asp           
            660                 665                 670                   

cat cag ctg ctg tgg gga gaa gcc ctg ctc atc aca cct gtt ctg cag       2064
His Gln Leu Leu Trp Gly Glu Ala Leu Leu Ile Thr Pro Val Leu Gln           
        675                 680                 685                       

gcc gga aag gcc gaa gtg acc ggc tat ttt cct ctc ggc act tgg tac       2112
Ala Gly Lys Ala Glu Val Thr Gly Tyr Phe Pro Leu Gly Thr Trp Tyr           
    690                 695                 700                           

gac ctg cag acc gtg cct att gag gcc ctg gga tct ctt cct cca cct       2160
Asp Leu Gln Thr Val Pro Ile Glu Ala Leu Gly Ser Leu Pro Pro Pro           
705                 710                 715                 720           

cct gct gct cct aga gag cct gcc atc cac tct gaa ggc cag tgg gtt       2208
Pro Ala Ala Pro Arg Glu Pro Ala Ile His Ser Glu Gly Gln Trp Val           
                725                 730                 735               

aca ctg ccc gct cct ctg gac acc atc aac gtg cac ctg aga gct ggc       2256
Thr Leu Pro Ala Pro Leu Asp Thr Ile Asn Val His Leu Arg Ala Gly           
            740                 745                 750                   

tac atc atc cca ctg caa ggc cct ggc ctg acc aca acc gaa tct aga       2304
Tyr Ile Ile Pro Leu Gln Gly Pro Gly Leu Thr Thr Thr Glu Ser Arg           
        755                 760                 765                       

cag cag ccc atg gct ctg gcc gtg gct ctt aca aaa ggc gga gag gct       2352
Gln Gln Pro Met Ala Leu Ala Val Ala Leu Thr Lys Gly Gly Glu Ala           
    770                 775                 780                           

aga ggc gag ctg ttc tgg gac gac ggc gag tct ctg gaa gtg ctg gaa       2400
Arg Gly Glu Leu Phe Trp Asp Asp Gly Glu Ser Leu Glu Val Leu Glu           
785                 790                 795                 800           

cgg ggc gct tat acc caa gtg atc ttc ctg gcc aga aac aac acc atc       2448
Arg Gly Ala Tyr Thr Gln Val Ile Phe Leu Ala Arg Asn Asn Thr Ile           
                805                 810                 815               

gtg aac gaa ctc gtg cgc gtg acc agt gaa ggt gct gga ctg caa ctg       2496
Val Asn Glu Leu Val Arg Val Thr Ser Glu Gly Ala Gly Leu Gln Leu           
            820                 825                 830                   

cag aaa gtg acc gtg ctc gga gtg gcc aca gct cct caa cag gtg ctg       2544
Gln Lys Val Thr Val Leu Gly Val Ala Thr Ala Pro Gln Gln Val Leu           
        835                 840                 845                       

tct aac ggc gtg ccc gtg tcc aac ttc aca tac agc ccc gac acc aag       2592
Ser Asn Gly Val Pro Val Ser Asn Phe Thr Tyr Ser Pro Asp Thr Lys           
    850                 855                 860                           

gtc ctg gac atc tgt gtg tca ctg ctg atg ggc gag cag ttc ctg gtg       2640
Val Leu Asp Ile Cys Val Ser Leu Leu Met Gly Glu Gln Phe Leu Val           
865                 870                 875                 880           

tcc tgg tgc                                                           2649
Ser Trp Cys                                                               
                                                                          


<210>  15
<211>  883
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  15

Ala His Pro Gly Arg Pro Arg Ala Val Pro Thr Gln Cys Asp Val Pro 
1               5                   10                  15      


Pro Asn Ser Arg Phe Asp Cys Ala Pro Asp Lys Ala Ile Thr Gln Glu 
            20                  25                  30          


Gln Cys Glu Ala Arg Gly Cys Cys Tyr Ile Pro Ala Lys Gln Gly Leu 
        35                  40                  45              


Gln Gly Ala Gln Met Gly Gln Pro Trp Cys Phe Phe Pro Pro Ser Tyr 
    50                  55                  60                  


Pro Ser Tyr Lys Leu Glu Asn Leu Ser Ser Ser Glu Met Gly Tyr Thr 
65                  70                  75                  80  


Ala Thr Leu Thr Arg Thr Thr Pro Thr Phe Phe Pro Lys Asp Ile Leu 
                85                  90                  95      


Thr Leu Arg Leu Asp Val Met Met Glu Thr Glu Asn Arg Leu His Phe 
            100                 105                 110         


Thr Ile Lys Asp Pro Ala Asn Arg Arg Tyr Glu Val Pro Leu Glu Thr 
        115                 120                 125             


Pro His Val His Ser Arg Ala Pro Ser Pro Leu Tyr Ser Val Glu Phe 
    130                 135                 140                 


Ser Glu Glu Pro Phe Gly Val Ile Val Arg Arg Gln Leu Asp Gly Arg 
145                 150                 155                 160 


Val Leu Leu Asn Thr Thr Val Ala Pro Leu Phe Phe Ala Asp Gln Phe 
                165                 170                 175     


Leu Gln Leu Ser Thr Ser Leu Pro Ser Gln Tyr Ile Thr Gly Leu Ala 
            180                 185                 190         


Glu His Leu Ser Pro Leu Met Leu Ser Thr Ser Trp Thr Arg Ile Thr 
        195                 200                 205             


Leu Trp Asn Arg Asp Leu Ala Pro Thr Pro Gly Ala Asn Leu Tyr Gly 
    210                 215                 220                 


Ser His Pro Phe Tyr Leu Ala Leu Glu Asp Gly Gly Ser Ala His Gly 
225                 230                 235                 240 


Val Phe Leu Leu Asn Ser Asn Ala Met Asp Val Val Leu Gln Pro Ser 
                245                 250                 255     


Pro Ala Leu Ser Trp Arg Ser Thr Gly Gly Ile Leu Asp Val Tyr Ile 
            260                 265                 270         


Phe Leu Gly Pro Glu Pro Lys Ser Val Val Gln Gln Tyr Leu Asp Val 
        275                 280                 285             


Val Gly Tyr Pro Phe Met Pro Pro Tyr Trp Gly Leu Gly Phe His Leu 
    290                 295                 300                 


Cys Arg Trp Gly Tyr Ser Ser Thr Ala Ile Thr Arg Gln Val Val Glu 
305                 310                 315                 320 


Asn Met Thr Arg Ala His Phe Pro Leu Asp Val Gln Trp Asn Asp Leu 
                325                 330                 335     


Asp Tyr Met Asp Ser Arg Arg Asp Phe Thr Phe Asn Lys Asp Gly Phe 
            340                 345                 350         


Arg Asp Phe Pro Ala Met Val Gln Glu Leu His Gln Gly Gly Arg Arg 
        355                 360                 365             


Tyr Met Met Ile Val Asp Pro Ala Ile Ser Ser Ser Gly Pro Ala Gly 
    370                 375                 380                 


Ser Tyr Arg Pro Tyr Asp Glu Gly Leu Arg Arg Gly Val Phe Ile Thr 
385                 390                 395                 400 


Asn Glu Thr Gly Gln Pro Leu Ile Gly Lys Val Trp Pro Gly Ser Thr 
                405                 410                 415     


Ala Phe Pro Asp Phe Thr Asn Pro Thr Ala Leu Ala Trp Trp Glu Asp 
            420                 425                 430         


Met Val Ala Glu Phe His Asp Gln Val Pro Phe Asp Gly Met Trp Ile 
        435                 440                 445             


Asp Met Asn Glu Pro Ser Asn Phe Ile Arg Gly Ser Glu Asp Gly Cys 
    450                 455                 460                 


Pro Asn Asn Glu Leu Glu Asn Pro Pro Tyr Val Pro Gly Val Val Gly 
465                 470                 475                 480 


Gly Thr Leu Gln Ala Ala Thr Ile Cys Ala Ser Ser His Gln Phe Leu 
                485                 490                 495     


Ser Thr His Tyr Asn Leu His Asn Leu Tyr Gly Leu Thr Glu Ala Ile 
            500                 505                 510         


Ala Ser His Arg Ala Leu Val Lys Ala Arg Gly Thr Arg Pro Phe Val 
        515                 520                 525             


Ile Ser Arg Ser Thr Phe Ala Gly His Gly Arg Tyr Ala Gly His Trp 
    530                 535                 540                 


Thr Gly Asp Val Trp Ser Ser Trp Glu Gln Leu Ala Ser Ser Val Pro 
545                 550                 555                 560 


Glu Ile Leu Gln Phe Asn Leu Leu Gly Val Pro Leu Val Gly Ala Asp 
                565                 570                 575     


Val Cys Gly Phe Leu Gly Asn Thr Ser Glu Glu Leu Cys Val Arg Trp 
            580                 585                 590         


Thr Gln Leu Gly Ala Phe Tyr Pro Phe Met Arg Asn His Asn Ser Leu 
        595                 600                 605             


Leu Ser Leu Pro Gln Glu Pro Tyr Ser Phe Ser Glu Pro Ala Gln Gln 
    610                 615                 620                 


Ala Met Arg Lys Ala Leu Thr Leu Arg Tyr Ala Leu Leu Pro His Leu 
625                 630                 635                 640 


Tyr Thr Leu Phe His Gln Ala His Val Ala Gly Glu Thr Val Ala Arg 
                645                 650                 655     


Pro Leu Phe Leu Glu Phe Pro Lys Asp Ser Ser Thr Trp Thr Val Asp 
            660                 665                 670         


His Gln Leu Leu Trp Gly Glu Ala Leu Leu Ile Thr Pro Val Leu Gln 
        675                 680                 685             


Ala Gly Lys Ala Glu Val Thr Gly Tyr Phe Pro Leu Gly Thr Trp Tyr 
    690                 695                 700                 


Asp Leu Gln Thr Val Pro Ile Glu Ala Leu Gly Ser Leu Pro Pro Pro 
705                 710                 715                 720 


Pro Ala Ala Pro Arg Glu Pro Ala Ile His Ser Glu Gly Gln Trp Val 
                725                 730                 735     


Thr Leu Pro Ala Pro Leu Asp Thr Ile Asn Val His Leu Arg Ala Gly 
            740                 745                 750         


Tyr Ile Ile Pro Leu Gln Gly Pro Gly Leu Thr Thr Thr Glu Ser Arg 
        755                 760                 765             


Gln Gln Pro Met Ala Leu Ala Val Ala Leu Thr Lys Gly Gly Glu Ala 
    770                 775                 780                 


Arg Gly Glu Leu Phe Trp Asp Asp Gly Glu Ser Leu Glu Val Leu Glu 
785                 790                 795                 800 


Arg Gly Ala Tyr Thr Gln Val Ile Phe Leu Ala Arg Asn Asn Thr Ile 
                805                 810                 815     


Val Asn Glu Leu Val Arg Val Thr Ser Glu Gly Ala Gly Leu Gln Leu 
            820                 825                 830         


Gln Lys Val Thr Val Leu Gly Val Ala Thr Ala Pro Gln Gln Val Leu 
        835                 840                 845             


Ser Asn Gly Val Pro Val Ser Asn Phe Thr Tyr Ser Pro Asp Thr Lys 
    850                 855                 860                 


Val Leu Asp Ile Cys Val Ser Leu Leu Met Gly Glu Gln Phe Leu Val 
865                 870                 875                 880 


Ser Trp Cys 
            


<210>  16
<211>  2304
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Engineered DNA for hGAA780I 123-890


<220>
<221>  CDS
<222>  (1)..(2304)

<400>  16
gga cag ccc tgg tgc ttc ttc cca cca tct tac ccc agc tac aag ctg         48
Gly Gln Pro Trp Cys Phe Phe Pro Pro Ser Tyr Pro Ser Tyr Lys Leu           
1               5                   10                  15                

gaa aac ctg agc agc agc gag atg ggc tac acc gcc aca ctg acc aga         96
Glu Asn Leu Ser Ser Ser Glu Met Gly Tyr Thr Ala Thr Leu Thr Arg           
            20                  25                  30                    

acc aca cct aca ttc ttc ccg aag gac atc ctg aca ctg cgg ctg gac        144
Thr Thr Pro Thr Phe Phe Pro Lys Asp Ile Leu Thr Leu Arg Leu Asp           
        35                  40                  45                        

gtg atg atg gaa acc gag aac cgg ctg cac ttc acc atc aag gac ccc        192
Val Met Met Glu Thr Glu Asn Arg Leu His Phe Thr Ile Lys Asp Pro           
    50                  55                  60                            

gcc aat cgg aga tac gag gtg cca ctg gaa acc cct cac gtg cac tct        240
Ala Asn Arg Arg Tyr Glu Val Pro Leu Glu Thr Pro His Val His Ser           
65                  70                  75                  80            

aga gcc cca tct cca ctg tac agc gtg gaa ttt tcc gag gaa ccc ttc        288
Arg Ala Pro Ser Pro Leu Tyr Ser Val Glu Phe Ser Glu Glu Pro Phe           
                85                  90                  95                

ggc gtg atc gtg cgg aga cag ctg gat gga aga gtg ctg ctg aac acc        336
Gly Val Ile Val Arg Arg Gln Leu Asp Gly Arg Val Leu Leu Asn Thr           
            100                 105                 110                   

aca gtg gcc cct ctg ttc ttc gcc gac cag ttt ctg cag ctg agc acc        384
Thr Val Ala Pro Leu Phe Phe Ala Asp Gln Phe Leu Gln Leu Ser Thr           
        115                 120                 125                       

agc ctg cct agc cag tat atc aca ggc ctg gcc gag cac ctg tct cca        432
Ser Leu Pro Ser Gln Tyr Ile Thr Gly Leu Ala Glu His Leu Ser Pro           
    130                 135                 140                           

ctg atg ctg agc aca tcc tgg acc aga atc acc ctg tgg aac aga gat        480
Leu Met Leu Ser Thr Ser Trp Thr Arg Ile Thr Leu Trp Asn Arg Asp           
145                 150                 155                 160           

ctg gcc cct aca cct ggc gcc aac ctg tac ggc tct cac ccc ttt tat        528
Leu Ala Pro Thr Pro Gly Ala Asn Leu Tyr Gly Ser His Pro Phe Tyr           
                165                 170                 175               

ctg gcc ctg gaa gac ggc gga tct gcc cac ggt gtt ttt ctg ctg aac        576
Leu Ala Leu Glu Asp Gly Gly Ser Ala His Gly Val Phe Leu Leu Asn           
            180                 185                 190                   

tcc aac gcc atg gac gtg gtg ctg cag cca tct cct gct ctg tct tgg        624
Ser Asn Ala Met Asp Val Val Leu Gln Pro Ser Pro Ala Leu Ser Trp           
        195                 200                 205                       

aga agc aca ggc ggc atc ctg gac gtg tac atc ttt ctg ggc ccc gag        672
Arg Ser Thr Gly Gly Ile Leu Asp Val Tyr Ile Phe Leu Gly Pro Glu           
    210                 215                 220                           

cct aag agc gtg gtg cag cag tat ctg gac gtc gtg ggc tac ccc ttc        720
Pro Lys Ser Val Val Gln Gln Tyr Leu Asp Val Val Gly Tyr Pro Phe           
225                 230                 235                 240           

atg cct cct tat tgg ggc ctg ggc ttc cac ctg tgc agg tgg gga tac        768
Met Pro Pro Tyr Trp Gly Leu Gly Phe His Leu Cys Arg Trp Gly Tyr           
                245                 250                 255               

agc agc acc gcc atc acc aga cag gtg gtg gaa aac atg acc cgg gct        816
Ser Ser Thr Ala Ile Thr Arg Gln Val Val Glu Asn Met Thr Arg Ala           
            260                 265                 270                   

cac ttc cca ctg gac gtg cag tgg aac gac ctg gac tac atg gac agc        864
His Phe Pro Leu Asp Val Gln Trp Asn Asp Leu Asp Tyr Met Asp Ser           
        275                 280                 285                       

aga cgg gac ttc acc ttc aac aag gac ggc ttc aga gac ttc ccc gcc        912
Arg Arg Asp Phe Thr Phe Asn Lys Asp Gly Phe Arg Asp Phe Pro Ala           
    290                 295                 300                           

atg gtg caa gaa ctg cac caa ggc ggc aga cgg tac atg atg att gtg        960
Met Val Gln Glu Leu His Gln Gly Gly Arg Arg Tyr Met Met Ile Val           
305                 310                 315                 320           

gac cca gcc atc agc tct agc ggc cct gcc gga agc tac aga cct tac       1008
Asp Pro Ala Ile Ser Ser Ser Gly Pro Ala Gly Ser Tyr Arg Pro Tyr           
                325                 330                 335               

gat gag ggc ctg aga aga ggc gtg ttc atc acc aac gag aca ggc cag       1056
Asp Glu Gly Leu Arg Arg Gly Val Phe Ile Thr Asn Glu Thr Gly Gln           
            340                 345                 350                   

cct ctg atc ggc aaa gtg tgg cct ggc agc aca gcc ttt cca gac ttc       1104
Pro Leu Ile Gly Lys Val Trp Pro Gly Ser Thr Ala Phe Pro Asp Phe           
        355                 360                 365                       

aca aac ccc acc gct ctg gct tgg tgg gaa gat atg gtg gcc gag ttc       1152
Thr Asn Pro Thr Ala Leu Ala Trp Trp Glu Asp Met Val Ala Glu Phe           
    370                 375                 380                           

cac gat cag gtg ccc ttc gat ggc atg tgg atc gac atg aac gag ccc       1200
His Asp Gln Val Pro Phe Asp Gly Met Trp Ile Asp Met Asn Glu Pro           
385                 390                 395                 400           

agc aac ttc atc cgg ggc agc gag gac ggc tgc ccc aac aac gaa ctg       1248
Ser Asn Phe Ile Arg Gly Ser Glu Asp Gly Cys Pro Asn Asn Glu Leu           
                405                 410                 415               

gaa aat cct cct tac gtg ccc ggc gtt gtc ggc gga aca ctt cag gcc       1296
Glu Asn Pro Pro Tyr Val Pro Gly Val Val Gly Gly Thr Leu Gln Ala           
            420                 425                 430                   

gct aca atc tgt gcc agc agc cac cag ttc ctc agc acc cac tac aac       1344
Ala Thr Ile Cys Ala Ser Ser His Gln Phe Leu Ser Thr His Tyr Asn           
        435                 440                 445                       

ctg cac aac ctg tac ggc ctg acc gag gcc att gcc tct cat aga gcc       1392
Leu His Asn Leu Tyr Gly Leu Thr Glu Ala Ile Ala Ser His Arg Ala           
    450                 455                 460                           

ctg gtt aag gcc aga ggc acc cgg cct ttt gtg atc agc aga agc aca       1440
Leu Val Lys Ala Arg Gly Thr Arg Pro Phe Val Ile Ser Arg Ser Thr           
465                 470                 475                 480           

ttc gcc ggc cac ggc aga tac gcc gga cat tgg aca ggc gac gtg tgg       1488
Phe Ala Gly His Gly Arg Tyr Ala Gly His Trp Thr Gly Asp Val Trp           
                485                 490                 495               

tct agt tgg gag cag ctg gct agc agc gtg cca gag atc ctg cag ttc       1536
Ser Ser Trp Glu Gln Leu Ala Ser Ser Val Pro Glu Ile Leu Gln Phe           
            500                 505                 510                   

aat ctg ctg ggc gtg cca ctc gtg gga gcc gac gtt tgt ggc ttc ctg       1584
Asn Leu Leu Gly Val Pro Leu Val Gly Ala Asp Val Cys Gly Phe Leu           
        515                 520                 525                       

ggc aac acc agc gag gaa ctg tgt gtg cgt tgg aca cag ctg ggc gcc       1632
Gly Asn Thr Ser Glu Glu Leu Cys Val Arg Trp Thr Gln Leu Gly Ala           
    530                 535                 540                           

ttc tat ccc ttc atg aga aac cac aac agc ctg ctg agc ctg cct caa       1680
Phe Tyr Pro Phe Met Arg Asn His Asn Ser Leu Leu Ser Leu Pro Gln           
545                 550                 555                 560           

gag ccc tac agc ttt agc gag cct gca cag cag gcc atg aga aag gcc       1728
Glu Pro Tyr Ser Phe Ser Glu Pro Ala Gln Gln Ala Met Arg Lys Ala           
                565                 570                 575               

ctg act ctg aga tac gct ctg ctg ccc cac ctg tac acc ctg ttt cac       1776
Leu Thr Leu Arg Tyr Ala Leu Leu Pro His Leu Tyr Thr Leu Phe His           
            580                 585                 590                   

cag gct cac gtg gcc ggg gag aca gtg gct aga cct ctg ttc ctg gaa       1824
Gln Ala His Val Ala Gly Glu Thr Val Ala Arg Pro Leu Phe Leu Glu           
        595                 600                 605                       

ttt ccc aag gac agc tcc acc tgg acc gtg gat cat cag ctg ctg tgg       1872
Phe Pro Lys Asp Ser Ser Thr Trp Thr Val Asp His Gln Leu Leu Trp           
    610                 615                 620                           

gga gaa gcc ctg ctc atc aca cct gtt ctg cag gcc gga aag gcc gaa       1920
Gly Glu Ala Leu Leu Ile Thr Pro Val Leu Gln Ala Gly Lys Ala Glu           
625                 630                 635                 640           

gtg acc ggc tat ttt cct ctc ggc act tgg tac gac ctg cag acc gtg       1968
Val Thr Gly Tyr Phe Pro Leu Gly Thr Trp Tyr Asp Leu Gln Thr Val           
                645                 650                 655               

cct att gag gcc ctg gga tct ctt cct cca cct cct gct gct cct aga       2016
Pro Ile Glu Ala Leu Gly Ser Leu Pro Pro Pro Pro Ala Ala Pro Arg           
            660                 665                 670                   

gag cct gcc atc cac tct gaa ggc cag tgg gtt aca ctg ccc gct cct       2064
Glu Pro Ala Ile His Ser Glu Gly Gln Trp Val Thr Leu Pro Ala Pro           
        675                 680                 685                       

ctg gac acc atc aac gtg cac ctg aga gct ggc tac atc atc cca ctg       2112
Leu Asp Thr Ile Asn Val His Leu Arg Ala Gly Tyr Ile Ile Pro Leu           
    690                 695                 700                           

caa ggc cct ggc ctg acc aca acc gaa tct aga cag cag ccc atg gct       2160
Gln Gly Pro Gly Leu Thr Thr Thr Glu Ser Arg Gln Gln Pro Met Ala           
705                 710                 715                 720           

ctg gcc gtg gct ctt aca aaa ggc gga gag gct aga ggc gag ctg ttc       2208
Leu Ala Val Ala Leu Thr Lys Gly Gly Glu Ala Arg Gly Glu Leu Phe           
                725                 730                 735               

tgg gac gac ggc gag tct ctg gaa gtg ctg gaa cgg ggc gct tat acc       2256
Trp Asp Asp Gly Glu Ser Leu Glu Val Leu Glu Arg Gly Ala Tyr Thr           
            740                 745                 750                   

caa gtg atc ttc ctg gcc aga aac aac acc atc gtg aac gaa ctc gtg       2304
Gln Val Ile Phe Leu Ala Arg Asn Asn Thr Ile Val Asn Glu Leu Val           
        755                 760                 765                       


<210>  17
<211>  768
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  17

Gly Gln Pro Trp Cys Phe Phe Pro Pro Ser Tyr Pro Ser Tyr Lys Leu 
1               5                   10                  15      


Glu Asn Leu Ser Ser Ser Glu Met Gly Tyr Thr Ala Thr Leu Thr Arg 
            20                  25                  30          


Thr Thr Pro Thr Phe Phe Pro Lys Asp Ile Leu Thr Leu Arg Leu Asp 
        35                  40                  45              


Val Met Met Glu Thr Glu Asn Arg Leu His Phe Thr Ile Lys Asp Pro 
    50                  55                  60                  


Ala Asn Arg Arg Tyr Glu Val Pro Leu Glu Thr Pro His Val His Ser 
65                  70                  75                  80  


Arg Ala Pro Ser Pro Leu Tyr Ser Val Glu Phe Ser Glu Glu Pro Phe 
                85                  90                  95      


Gly Val Ile Val Arg Arg Gln Leu Asp Gly Arg Val Leu Leu Asn Thr 
            100                 105                 110         


Thr Val Ala Pro Leu Phe Phe Ala Asp Gln Phe Leu Gln Leu Ser Thr 
        115                 120                 125             


Ser Leu Pro Ser Gln Tyr Ile Thr Gly Leu Ala Glu His Leu Ser Pro 
    130                 135                 140                 


Leu Met Leu Ser Thr Ser Trp Thr Arg Ile Thr Leu Trp Asn Arg Asp 
145                 150                 155                 160 


Leu Ala Pro Thr Pro Gly Ala Asn Leu Tyr Gly Ser His Pro Phe Tyr 
                165                 170                 175     


Leu Ala Leu Glu Asp Gly Gly Ser Ala His Gly Val Phe Leu Leu Asn 
            180                 185                 190         


Ser Asn Ala Met Asp Val Val Leu Gln Pro Ser Pro Ala Leu Ser Trp 
        195                 200                 205             


Arg Ser Thr Gly Gly Ile Leu Asp Val Tyr Ile Phe Leu Gly Pro Glu 
    210                 215                 220                 


Pro Lys Ser Val Val Gln Gln Tyr Leu Asp Val Val Gly Tyr Pro Phe 
225                 230                 235                 240 


Met Pro Pro Tyr Trp Gly Leu Gly Phe His Leu Cys Arg Trp Gly Tyr 
                245                 250                 255     


Ser Ser Thr Ala Ile Thr Arg Gln Val Val Glu Asn Met Thr Arg Ala 
            260                 265                 270         


His Phe Pro Leu Asp Val Gln Trp Asn Asp Leu Asp Tyr Met Asp Ser 
        275                 280                 285             


Arg Arg Asp Phe Thr Phe Asn Lys Asp Gly Phe Arg Asp Phe Pro Ala 
    290                 295                 300                 


Met Val Gln Glu Leu His Gln Gly Gly Arg Arg Tyr Met Met Ile Val 
305                 310                 315                 320 


Asp Pro Ala Ile Ser Ser Ser Gly Pro Ala Gly Ser Tyr Arg Pro Tyr 
                325                 330                 335     


Asp Glu Gly Leu Arg Arg Gly Val Phe Ile Thr Asn Glu Thr Gly Gln 
            340                 345                 350         


Pro Leu Ile Gly Lys Val Trp Pro Gly Ser Thr Ala Phe Pro Asp Phe 
        355                 360                 365             


Thr Asn Pro Thr Ala Leu Ala Trp Trp Glu Asp Met Val Ala Glu Phe 
    370                 375                 380                 


His Asp Gln Val Pro Phe Asp Gly Met Trp Ile Asp Met Asn Glu Pro 
385                 390                 395                 400 


Ser Asn Phe Ile Arg Gly Ser Glu Asp Gly Cys Pro Asn Asn Glu Leu 
                405                 410                 415     


Glu Asn Pro Pro Tyr Val Pro Gly Val Val Gly Gly Thr Leu Gln Ala 
            420                 425                 430         


Ala Thr Ile Cys Ala Ser Ser His Gln Phe Leu Ser Thr His Tyr Asn 
        435                 440                 445             


Leu His Asn Leu Tyr Gly Leu Thr Glu Ala Ile Ala Ser His Arg Ala 
    450                 455                 460                 


Leu Val Lys Ala Arg Gly Thr Arg Pro Phe Val Ile Ser Arg Ser Thr 
465                 470                 475                 480 


Phe Ala Gly His Gly Arg Tyr Ala Gly His Trp Thr Gly Asp Val Trp 
                485                 490                 495     


Ser Ser Trp Glu Gln Leu Ala Ser Ser Val Pro Glu Ile Leu Gln Phe 
            500                 505                 510         


Asn Leu Leu Gly Val Pro Leu Val Gly Ala Asp Val Cys Gly Phe Leu 
        515                 520                 525             


Gly Asn Thr Ser Glu Glu Leu Cys Val Arg Trp Thr Gln Leu Gly Ala 
    530                 535                 540                 


Phe Tyr Pro Phe Met Arg Asn His Asn Ser Leu Leu Ser Leu Pro Gln 
545                 550                 555                 560 


Glu Pro Tyr Ser Phe Ser Glu Pro Ala Gln Gln Ala Met Arg Lys Ala 
                565                 570                 575     


Leu Thr Leu Arg Tyr Ala Leu Leu Pro His Leu Tyr Thr Leu Phe His 
            580                 585                 590         


Gln Ala His Val Ala Gly Glu Thr Val Ala Arg Pro Leu Phe Leu Glu 
        595                 600                 605             


Phe Pro Lys Asp Ser Ser Thr Trp Thr Val Asp His Gln Leu Leu Trp 
    610                 615                 620                 


Gly Glu Ala Leu Leu Ile Thr Pro Val Leu Gln Ala Gly Lys Ala Glu 
625                 630                 635                 640 


Val Thr Gly Tyr Phe Pro Leu Gly Thr Trp Tyr Asp Leu Gln Thr Val 
                645                 650                 655     


Pro Ile Glu Ala Leu Gly Ser Leu Pro Pro Pro Pro Ala Ala Pro Arg 
            660                 665                 670         


Glu Pro Ala Ile His Ser Glu Gly Gln Trp Val Thr Leu Pro Ala Pro 
        675                 680                 685             


Leu Asp Thr Ile Asn Val His Leu Arg Ala Gly Tyr Ile Ile Pro Leu 
    690                 695                 700                 


Gln Gly Pro Gly Leu Thr Thr Thr Glu Ser Arg Gln Gln Pro Met Ala 
705                 710                 715                 720 


Leu Ala Val Ala Leu Thr Lys Gly Gly Glu Ala Arg Gly Glu Leu Phe 
                725                 730                 735     


Trp Asp Asp Gly Glu Ser Leu Glu Val Leu Glu Arg Gly Ala Tyr Thr 
            740                 745                 750         


Gln Val Ile Phe Leu Ala Arg Asn Asn Thr Ile Val Asn Glu Leu Val 
        755                 760                 765             


<210>  18
<211>  2247
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Engineered hGAA 70kD cDNA


<220>
<221>  CDS
<222>  (1)..(2247)

<400>  18
gcc cca tct cca ctg tac agc gtg gaa ttt tcc gag gaa ccc ttc ggc         48
Ala Pro Ser Pro Leu Tyr Ser Val Glu Phe Ser Glu Glu Pro Phe Gly           
1               5                   10                  15                

gtg atc gtg cgg aga cag ctg gat gga aga gtg ctg ctg aac acc aca         96
Val Ile Val Arg Arg Gln Leu Asp Gly Arg Val Leu Leu Asn Thr Thr           
            20                  25                  30                    

gtg gcc cct ctg ttc ttc gcc gac cag ttt ctg cag ctg agc acc agc        144
Val Ala Pro Leu Phe Phe Ala Asp Gln Phe Leu Gln Leu Ser Thr Ser           
        35                  40                  45                        

ctg cct agc cag tat atc aca ggc ctg gcc gag cac ctg tct cca ctg        192
Leu Pro Ser Gln Tyr Ile Thr Gly Leu Ala Glu His Leu Ser Pro Leu           
    50                  55                  60                            

atg ctg agc aca tcc tgg acc aga atc acc ctg tgg aac aga gat ctg        240
Met Leu Ser Thr Ser Trp Thr Arg Ile Thr Leu Trp Asn Arg Asp Leu           
65                  70                  75                  80            

gcc cct aca cct ggc gcc aac ctg tac ggc tct cac ccc ttt tat ctg        288
Ala Pro Thr Pro Gly Ala Asn Leu Tyr Gly Ser His Pro Phe Tyr Leu           
                85                  90                  95                

gcc ctg gaa gac ggc gga tct gcc cac ggt gtt ttt ctg ctg aac tcc        336
Ala Leu Glu Asp Gly Gly Ser Ala His Gly Val Phe Leu Leu Asn Ser           
            100                 105                 110                   

aac gcc atg gac gtg gtg ctg cag cca tct cct gct ctg tct tgg aga        384
Asn Ala Met Asp Val Val Leu Gln Pro Ser Pro Ala Leu Ser Trp Arg           
        115                 120                 125                       

agc aca ggc ggc atc ctg gac gtg tac atc ttt ctg ggc ccc gag cct        432
Ser Thr Gly Gly Ile Leu Asp Val Tyr Ile Phe Leu Gly Pro Glu Pro           
    130                 135                 140                           

aag agc gtg gtg cag cag tat ctg gac gtc gtg ggc tac ccc ttc atg        480
Lys Ser Val Val Gln Gln Tyr Leu Asp Val Val Gly Tyr Pro Phe Met           
145                 150                 155                 160           

cct cct tat tgg ggc ctg ggc ttc cac ctg tgc agg tgg gga tac agc        528
Pro Pro Tyr Trp Gly Leu Gly Phe His Leu Cys Arg Trp Gly Tyr Ser           
                165                 170                 175               

agc acc gcc atc acc aga cag gtg gtg gaa aac atg acc cgg gct cac        576
Ser Thr Ala Ile Thr Arg Gln Val Val Glu Asn Met Thr Arg Ala His           
            180                 185                 190                   

ttc cca ctg gac gtg cag tgg aac gac ctg gac tac atg gac agc aga        624
Phe Pro Leu Asp Val Gln Trp Asn Asp Leu Asp Tyr Met Asp Ser Arg           
        195                 200                 205                       

cgg gac ttc acc ttc aac aag gac ggc ttc aga gac ttc ccc gcc atg        672
Arg Asp Phe Thr Phe Asn Lys Asp Gly Phe Arg Asp Phe Pro Ala Met           
    210                 215                 220                           

gtg caa gaa ctg cac caa ggc ggc aga cgg tac atg atg att gtg gac        720
Val Gln Glu Leu His Gln Gly Gly Arg Arg Tyr Met Met Ile Val Asp           
225                 230                 235                 240           

cca gcc atc agc tct agc ggc cct gcc gga agc tac aga cct tac gat        768
Pro Ala Ile Ser Ser Ser Gly Pro Ala Gly Ser Tyr Arg Pro Tyr Asp           
                245                 250                 255               

gag ggc ctg aga aga ggc gtg ttc atc acc aac gag aca ggc cag cct        816
Glu Gly Leu Arg Arg Gly Val Phe Ile Thr Asn Glu Thr Gly Gln Pro           
            260                 265                 270                   

ctg atc ggc aaa gtg tgg cct ggc agc aca gcc ttt cca gac ttc aca        864
Leu Ile Gly Lys Val Trp Pro Gly Ser Thr Ala Phe Pro Asp Phe Thr           
        275                 280                 285                       

aac ccc acc gct ctg gct tgg tgg gaa gat atg gtg gcc gag ttc cac        912
Asn Pro Thr Ala Leu Ala Trp Trp Glu Asp Met Val Ala Glu Phe His           
    290                 295                 300                           

gat cag gtg ccc ttc gat ggc atg tgg atc gac atg aac gag ccc agc        960
Asp Gln Val Pro Phe Asp Gly Met Trp Ile Asp Met Asn Glu Pro Ser           
305                 310                 315                 320           

aac ttc atc cgg ggc agc gag gac ggc tgc ccc aac aac gaa ctg gaa       1008
Asn Phe Ile Arg Gly Ser Glu Asp Gly Cys Pro Asn Asn Glu Leu Glu           
                325                 330                 335               

aat cct cct tac gtg ccc ggc gtt gtc ggc gga aca ctt cag gcc gct       1056
Asn Pro Pro Tyr Val Pro Gly Val Val Gly Gly Thr Leu Gln Ala Ala           
            340                 345                 350                   

aca atc tgt gcc agc agc cac cag ttc ctc agc acc cac tac aac ctg       1104
Thr Ile Cys Ala Ser Ser His Gln Phe Leu Ser Thr His Tyr Asn Leu           
        355                 360                 365                       

cac aac ctg tac ggc ctg acc gag gcc att gcc tct cat aga gcc ctg       1152
His Asn Leu Tyr Gly Leu Thr Glu Ala Ile Ala Ser His Arg Ala Leu           
    370                 375                 380                           

gtt aag gcc aga ggc acc cgg cct ttt gtg atc agc aga agc aca ttc       1200
Val Lys Ala Arg Gly Thr Arg Pro Phe Val Ile Ser Arg Ser Thr Phe           
385                 390                 395                 400           

gcc ggc cac ggc aga tac gcc gga cat tgg aca ggc gac gtg tgg tct       1248
Ala Gly His Gly Arg Tyr Ala Gly His Trp Thr Gly Asp Val Trp Ser           
                405                 410                 415               

agt tgg gag cag ctg gct agc agc gtg cca gag atc ctg cag ttc aat       1296
Ser Trp Glu Gln Leu Ala Ser Ser Val Pro Glu Ile Leu Gln Phe Asn           
            420                 425                 430                   

ctg ctg ggc gtg cca ctc gtg gga gcc gac gtt tgt ggc ttc ctg ggc       1344
Leu Leu Gly Val Pro Leu Val Gly Ala Asp Val Cys Gly Phe Leu Gly           
        435                 440                 445                       

aac acc agc gag gaa ctg tgt gtg cgt tgg aca cag ctg ggc gcc ttc       1392
Asn Thr Ser Glu Glu Leu Cys Val Arg Trp Thr Gln Leu Gly Ala Phe           
    450                 455                 460                           

tat ccc ttc atg aga aac cac aac agc ctg ctg agc ctg cct caa gag       1440
Tyr Pro Phe Met Arg Asn His Asn Ser Leu Leu Ser Leu Pro Gln Glu           
465                 470                 475                 480           

ccc tac agc ttt agc gag cct gca cag cag gcc atg aga aag gcc ctg       1488
Pro Tyr Ser Phe Ser Glu Pro Ala Gln Gln Ala Met Arg Lys Ala Leu           
                485                 490                 495               

act ctg aga tac gct ctg ctg ccc cac ctg tac acc ctg ttt cac cag       1536
Thr Leu Arg Tyr Ala Leu Leu Pro His Leu Tyr Thr Leu Phe His Gln           
            500                 505                 510                   

gct cac gtg gcc ggg gag aca gtg gct aga cct ctg ttc ctg gaa ttt       1584
Ala His Val Ala Gly Glu Thr Val Ala Arg Pro Leu Phe Leu Glu Phe           
        515                 520                 525                       

ccc aag gac agc tcc acc tgg acc gtg gat cat cag ctg ctg tgg gga       1632
Pro Lys Asp Ser Ser Thr Trp Thr Val Asp His Gln Leu Leu Trp Gly           
    530                 535                 540                           

gaa gcc ctg ctc atc aca cct gtt ctg cag gcc gga aag gcc gaa gtg       1680
Glu Ala Leu Leu Ile Thr Pro Val Leu Gln Ala Gly Lys Ala Glu Val           
545                 550                 555                 560           

acc ggc tat ttt cct ctc ggc act tgg tac gac ctg cag acc gtg cct       1728
Thr Gly Tyr Phe Pro Leu Gly Thr Trp Tyr Asp Leu Gln Thr Val Pro           
                565                 570                 575               

att gag gcc ctg gga tct ctt cct cca cct cct gct gct cct aga gag       1776
Ile Glu Ala Leu Gly Ser Leu Pro Pro Pro Pro Ala Ala Pro Arg Glu           
            580                 585                 590                   

cct gcc atc cac tct gaa ggc cag tgg gtt aca ctg ccc gct cct ctg       1824
Pro Ala Ile His Ser Glu Gly Gln Trp Val Thr Leu Pro Ala Pro Leu           
        595                 600                 605                       

gac acc atc aac gtg cac ctg aga gct ggc tac atc atc cca ctg caa       1872
Asp Thr Ile Asn Val His Leu Arg Ala Gly Tyr Ile Ile Pro Leu Gln           
    610                 615                 620                           

ggc cct ggc ctg acc aca acc gaa tct aga cag cag ccc atg gct ctg       1920
Gly Pro Gly Leu Thr Thr Thr Glu Ser Arg Gln Gln Pro Met Ala Leu           
625                 630                 635                 640           

gcc gtg gct ctt aca aaa ggc gga gag gct aga ggc gag ctg ttc tgg       1968
Ala Val Ala Leu Thr Lys Gly Gly Glu Ala Arg Gly Glu Leu Phe Trp           
                645                 650                 655               

gac gac ggc gag tct ctg gaa gtg ctg gaa cgg ggc gct tat acc caa       2016
Asp Asp Gly Glu Ser Leu Glu Val Leu Glu Arg Gly Ala Tyr Thr Gln           
            660                 665                 670                   

gtg atc ttc ctg gcc aga aac aac acc atc gtg aac gaa ctc gtg cgc       2064
Val Ile Phe Leu Ala Arg Asn Asn Thr Ile Val Asn Glu Leu Val Arg           
        675                 680                 685                       

gtg acc agt gaa ggt gct gga ctg caa ctg cag aaa gtg acc gtg ctc       2112
Val Thr Ser Glu Gly Ala Gly Leu Gln Leu Gln Lys Val Thr Val Leu           
    690                 695                 700                           

gga gtg gcc aca gct cct caa cag gtg ctg tct aac ggc gtg ccc gtg       2160
Gly Val Ala Thr Ala Pro Gln Gln Val Leu Ser Asn Gly Val Pro Val           
705                 710                 715                 720           

tcc aac ttc aca tac agc ccc gac acc aag gtc ctg gac atc tgt gtg       2208
Ser Asn Phe Thr Tyr Ser Pro Asp Thr Lys Val Leu Asp Ile Cys Val           
                725                 730                 735               

tca ctg ctg atg ggc gag cag ttc ctg gtg tcc tgg tgc                   2247
Ser Leu Leu Met Gly Glu Gln Phe Leu Val Ser Trp Cys                       
            740                 745                                       


<210>  19
<211>  749
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  19

Ala Pro Ser Pro Leu Tyr Ser Val Glu Phe Ser Glu Glu Pro Phe Gly 
1               5                   10                  15      


Val Ile Val Arg Arg Gln Leu Asp Gly Arg Val Leu Leu Asn Thr Thr 
            20                  25                  30          


Val Ala Pro Leu Phe Phe Ala Asp Gln Phe Leu Gln Leu Ser Thr Ser 
        35                  40                  45              


Leu Pro Ser Gln Tyr Ile Thr Gly Leu Ala Glu His Leu Ser Pro Leu 
    50                  55                  60                  


Met Leu Ser Thr Ser Trp Thr Arg Ile Thr Leu Trp Asn Arg Asp Leu 
65                  70                  75                  80  


Ala Pro Thr Pro Gly Ala Asn Leu Tyr Gly Ser His Pro Phe Tyr Leu 
                85                  90                  95      


Ala Leu Glu Asp Gly Gly Ser Ala His Gly Val Phe Leu Leu Asn Ser 
            100                 105                 110         


Asn Ala Met Asp Val Val Leu Gln Pro Ser Pro Ala Leu Ser Trp Arg 
        115                 120                 125             


Ser Thr Gly Gly Ile Leu Asp Val Tyr Ile Phe Leu Gly Pro Glu Pro 
    130                 135                 140                 


Lys Ser Val Val Gln Gln Tyr Leu Asp Val Val Gly Tyr Pro Phe Met 
145                 150                 155                 160 


Pro Pro Tyr Trp Gly Leu Gly Phe His Leu Cys Arg Trp Gly Tyr Ser 
                165                 170                 175     


Ser Thr Ala Ile Thr Arg Gln Val Val Glu Asn Met Thr Arg Ala His 
            180                 185                 190         


Phe Pro Leu Asp Val Gln Trp Asn Asp Leu Asp Tyr Met Asp Ser Arg 
        195                 200                 205             


Arg Asp Phe Thr Phe Asn Lys Asp Gly Phe Arg Asp Phe Pro Ala Met 
    210                 215                 220                 


Val Gln Glu Leu His Gln Gly Gly Arg Arg Tyr Met Met Ile Val Asp 
225                 230                 235                 240 


Pro Ala Ile Ser Ser Ser Gly Pro Ala Gly Ser Tyr Arg Pro Tyr Asp 
                245                 250                 255     


Glu Gly Leu Arg Arg Gly Val Phe Ile Thr Asn Glu Thr Gly Gln Pro 
            260                 265                 270         


Leu Ile Gly Lys Val Trp Pro Gly Ser Thr Ala Phe Pro Asp Phe Thr 
        275                 280                 285             


Asn Pro Thr Ala Leu Ala Trp Trp Glu Asp Met Val Ala Glu Phe His 
    290                 295                 300                 


Asp Gln Val Pro Phe Asp Gly Met Trp Ile Asp Met Asn Glu Pro Ser 
305                 310                 315                 320 


Asn Phe Ile Arg Gly Ser Glu Asp Gly Cys Pro Asn Asn Glu Leu Glu 
                325                 330                 335     


Asn Pro Pro Tyr Val Pro Gly Val Val Gly Gly Thr Leu Gln Ala Ala 
            340                 345                 350         


Thr Ile Cys Ala Ser Ser His Gln Phe Leu Ser Thr His Tyr Asn Leu 
        355                 360                 365             


His Asn Leu Tyr Gly Leu Thr Glu Ala Ile Ala Ser His Arg Ala Leu 
    370                 375                 380                 


Val Lys Ala Arg Gly Thr Arg Pro Phe Val Ile Ser Arg Ser Thr Phe 
385                 390                 395                 400 


Ala Gly His Gly Arg Tyr Ala Gly His Trp Thr Gly Asp Val Trp Ser 
                405                 410                 415     


Ser Trp Glu Gln Leu Ala Ser Ser Val Pro Glu Ile Leu Gln Phe Asn 
            420                 425                 430         


Leu Leu Gly Val Pro Leu Val Gly Ala Asp Val Cys Gly Phe Leu Gly 
        435                 440                 445             


Asn Thr Ser Glu Glu Leu Cys Val Arg Trp Thr Gln Leu Gly Ala Phe 
    450                 455                 460                 


Tyr Pro Phe Met Arg Asn His Asn Ser Leu Leu Ser Leu Pro Gln Glu 
465                 470                 475                 480 


Pro Tyr Ser Phe Ser Glu Pro Ala Gln Gln Ala Met Arg Lys Ala Leu 
                485                 490                 495     


Thr Leu Arg Tyr Ala Leu Leu Pro His Leu Tyr Thr Leu Phe His Gln 
            500                 505                 510         


Ala His Val Ala Gly Glu Thr Val Ala Arg Pro Leu Phe Leu Glu Phe 
        515                 520                 525             


Pro Lys Asp Ser Ser Thr Trp Thr Val Asp His Gln Leu Leu Trp Gly 
    530                 535                 540                 


Glu Ala Leu Leu Ile Thr Pro Val Leu Gln Ala Gly Lys Ala Glu Val 
545                 550                 555                 560 


Thr Gly Tyr Phe Pro Leu Gly Thr Trp Tyr Asp Leu Gln Thr Val Pro 
                565                 570                 575     


Ile Glu Ala Leu Gly Ser Leu Pro Pro Pro Pro Ala Ala Pro Arg Glu 
            580                 585                 590         


Pro Ala Ile His Ser Glu Gly Gln Trp Val Thr Leu Pro Ala Pro Leu 
        595                 600                 605             


Asp Thr Ile Asn Val His Leu Arg Ala Gly Tyr Ile Ile Pro Leu Gln 
    610                 615                 620                 


Gly Pro Gly Leu Thr Thr Thr Glu Ser Arg Gln Gln Pro Met Ala Leu 
625                 630                 635                 640 


Ala Val Ala Leu Thr Lys Gly Gly Glu Ala Arg Gly Glu Leu Phe Trp 
                645                 650                 655     


Asp Asp Gly Glu Ser Leu Glu Val Leu Glu Arg Gly Ala Tyr Thr Gln 
            660                 665                 670         


Val Ile Phe Leu Ala Arg Asn Asn Thr Ile Val Asn Glu Leu Val Arg 
        675                 680                 685             


Val Thr Ser Glu Gly Ala Gly Leu Gln Leu Gln Lys Val Thr Val Leu 
    690                 695                 700                 


Gly Val Ala Thr Ala Pro Gln Gln Val Leu Ser Asn Gly Val Pro Val 
705                 710                 715                 720 


Ser Asn Phe Thr Tyr Ser Pro Asp Thr Lys Val Leu Asp Ile Cys Val 
                725                 730                 735     


Ser Leu Leu Met Gly Glu Gln Phe Leu Val Ser Trp Cys 
            740                 745                 


<210>  20
<211>  2490
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered DNA for hGAAV780I 76 kD protein


<220>
<221>  CDS
<222>  (1)..(2490)

<400>  20
gga cag ccc tgg tgc ttc ttc cca cca tct tac ccc agc tac aag ctg         48
Gly Gln Pro Trp Cys Phe Phe Pro Pro Ser Tyr Pro Ser Tyr Lys Leu           
1               5                   10                  15                

gaa aac ctg agc agc agc gag atg ggc tac acc gcc aca ctg acc aga         96
Glu Asn Leu Ser Ser Ser Glu Met Gly Tyr Thr Ala Thr Leu Thr Arg           
            20                  25                  30                    

acc aca cct aca ttc ttc ccg aag gac atc ctg aca ctg cgg ctg gac        144
Thr Thr Pro Thr Phe Phe Pro Lys Asp Ile Leu Thr Leu Arg Leu Asp           
        35                  40                  45                        

gtg atg atg gaa acc gag aac cgg ctg cac ttc acc atc aag gac ccc        192
Val Met Met Glu Thr Glu Asn Arg Leu His Phe Thr Ile Lys Asp Pro           
    50                  55                  60                            

gcc aat cgg aga tac gag gtg cca ctg gaa acc cct cac gtg cac tct        240
Ala Asn Arg Arg Tyr Glu Val Pro Leu Glu Thr Pro His Val His Ser           
65                  70                  75                  80            

aga gcc cca tct cca ctg tac agc gtg gaa ttt tcc gag gaa ccc ttc        288
Arg Ala Pro Ser Pro Leu Tyr Ser Val Glu Phe Ser Glu Glu Pro Phe           
                85                  90                  95                

ggc gtg atc gtg cgg aga cag ctg gat gga aga gtg ctg ctg aac acc        336
Gly Val Ile Val Arg Arg Gln Leu Asp Gly Arg Val Leu Leu Asn Thr           
            100                 105                 110                   

aca gtg gcc cct ctg ttc ttc gcc gac cag ttt ctg cag ctg agc acc        384
Thr Val Ala Pro Leu Phe Phe Ala Asp Gln Phe Leu Gln Leu Ser Thr           
        115                 120                 125                       

agc ctg cct agc cag tat atc aca ggc ctg gcc gag cac ctg tct cca        432
Ser Leu Pro Ser Gln Tyr Ile Thr Gly Leu Ala Glu His Leu Ser Pro           
    130                 135                 140                           

ctg atg ctg agc aca tcc tgg acc aga atc acc ctg tgg aac aga gat        480
Leu Met Leu Ser Thr Ser Trp Thr Arg Ile Thr Leu Trp Asn Arg Asp           
145                 150                 155                 160           

ctg gcc cct aca cct ggc gcc aac ctg tac ggc tct cac ccc ttt tat        528
Leu Ala Pro Thr Pro Gly Ala Asn Leu Tyr Gly Ser His Pro Phe Tyr           
                165                 170                 175               

ctg gcc ctg gaa gac ggc gga tct gcc cac ggt gtt ttt ctg ctg aac        576
Leu Ala Leu Glu Asp Gly Gly Ser Ala His Gly Val Phe Leu Leu Asn           
            180                 185                 190                   

tcc aac gcc atg gac gtg gtg ctg cag cca tct cct gct ctg tct tgg        624
Ser Asn Ala Met Asp Val Val Leu Gln Pro Ser Pro Ala Leu Ser Trp           
        195                 200                 205                       

aga agc aca ggc ggc atc ctg gac gtg tac atc ttt ctg ggc ccc gag        672
Arg Ser Thr Gly Gly Ile Leu Asp Val Tyr Ile Phe Leu Gly Pro Glu           
    210                 215                 220                           

cct aag agc gtg gtg cag cag tat ctg gac gtc gtg ggc tac ccc ttc        720
Pro Lys Ser Val Val Gln Gln Tyr Leu Asp Val Val Gly Tyr Pro Phe           
225                 230                 235                 240           

atg cct cct tat tgg ggc ctg ggc ttc cac ctg tgc agg tgg gga tac        768
Met Pro Pro Tyr Trp Gly Leu Gly Phe His Leu Cys Arg Trp Gly Tyr           
                245                 250                 255               

agc agc acc gcc atc acc aga cag gtg gtg gaa aac atg acc cgg gct        816
Ser Ser Thr Ala Ile Thr Arg Gln Val Val Glu Asn Met Thr Arg Ala           
            260                 265                 270                   

cac ttc cca ctg gac gtg cag tgg aac gac ctg gac tac atg gac agc        864
His Phe Pro Leu Asp Val Gln Trp Asn Asp Leu Asp Tyr Met Asp Ser           
        275                 280                 285                       

aga cgg gac ttc acc ttc aac aag gac ggc ttc aga gac ttc ccc gcc        912
Arg Arg Asp Phe Thr Phe Asn Lys Asp Gly Phe Arg Asp Phe Pro Ala           
    290                 295                 300                           

atg gtg caa gaa ctg cac caa ggc ggc aga cgg tac atg atg att gtg        960
Met Val Gln Glu Leu His Gln Gly Gly Arg Arg Tyr Met Met Ile Val           
305                 310                 315                 320           

gac cca gcc atc agc tct agc ggc cct gcc gga agc tac aga cct tac       1008
Asp Pro Ala Ile Ser Ser Ser Gly Pro Ala Gly Ser Tyr Arg Pro Tyr           
                325                 330                 335               

gat gag ggc ctg aga aga ggc gtg ttc atc acc aac gag aca ggc cag       1056
Asp Glu Gly Leu Arg Arg Gly Val Phe Ile Thr Asn Glu Thr Gly Gln           
            340                 345                 350                   

cct ctg atc ggc aaa gtg tgg cct ggc agc aca gcc ttt cca gac ttc       1104
Pro Leu Ile Gly Lys Val Trp Pro Gly Ser Thr Ala Phe Pro Asp Phe           
        355                 360                 365                       

aca aac ccc acc gct ctg gct tgg tgg gaa gat atg gtg gcc gag ttc       1152
Thr Asn Pro Thr Ala Leu Ala Trp Trp Glu Asp Met Val Ala Glu Phe           
    370                 375                 380                           

cac gat cag gtg ccc ttc gat ggc atg tgg atc gac atg aac gag ccc       1200
His Asp Gln Val Pro Phe Asp Gly Met Trp Ile Asp Met Asn Glu Pro           
385                 390                 395                 400           

agc aac ttc atc cgg ggc agc gag gac ggc tgc ccc aac aac gaa ctg       1248
Ser Asn Phe Ile Arg Gly Ser Glu Asp Gly Cys Pro Asn Asn Glu Leu           
                405                 410                 415               

gaa aat cct cct tac gtg ccc ggc gtt gtc ggc gga aca ctt cag gcc       1296
Glu Asn Pro Pro Tyr Val Pro Gly Val Val Gly Gly Thr Leu Gln Ala           
            420                 425                 430                   

gct aca atc tgt gcc agc agc cac cag ttc ctc agc acc cac tac aac       1344
Ala Thr Ile Cys Ala Ser Ser His Gln Phe Leu Ser Thr His Tyr Asn           
        435                 440                 445                       

ctg cac aac ctg tac ggc ctg acc gag gcc att gcc tct cat aga gcc       1392
Leu His Asn Leu Tyr Gly Leu Thr Glu Ala Ile Ala Ser His Arg Ala           
    450                 455                 460                           

ctg gtt aag gcc aga ggc acc cgg cct ttt gtg atc agc aga agc aca       1440
Leu Val Lys Ala Arg Gly Thr Arg Pro Phe Val Ile Ser Arg Ser Thr           
465                 470                 475                 480           

ttc gcc ggc cac ggc aga tac gcc gga cat tgg aca ggc gac gtg tgg       1488
Phe Ala Gly His Gly Arg Tyr Ala Gly His Trp Thr Gly Asp Val Trp           
                485                 490                 495               

tct agt tgg gag cag ctg gct agc agc gtg cca gag atc ctg cag ttc       1536
Ser Ser Trp Glu Gln Leu Ala Ser Ser Val Pro Glu Ile Leu Gln Phe           
            500                 505                 510                   

aat ctg ctg ggc gtg cca ctc gtg gga gcc gac gtt tgt ggc ttc ctg       1584
Asn Leu Leu Gly Val Pro Leu Val Gly Ala Asp Val Cys Gly Phe Leu           
        515                 520                 525                       

ggc aac acc agc gag gaa ctg tgt gtg cgt tgg aca cag ctg ggc gcc       1632
Gly Asn Thr Ser Glu Glu Leu Cys Val Arg Trp Thr Gln Leu Gly Ala           
    530                 535                 540                           

ttc tat ccc ttc atg aga aac cac aac agc ctg ctg agc ctg cct caa       1680
Phe Tyr Pro Phe Met Arg Asn His Asn Ser Leu Leu Ser Leu Pro Gln           
545                 550                 555                 560           

gag ccc tac agc ttt agc gag cct gca cag cag gcc atg aga aag gcc       1728
Glu Pro Tyr Ser Phe Ser Glu Pro Ala Gln Gln Ala Met Arg Lys Ala           
                565                 570                 575               

ctg act ctg aga tac gct ctg ctg ccc cac ctg tac acc ctg ttt cac       1776
Leu Thr Leu Arg Tyr Ala Leu Leu Pro His Leu Tyr Thr Leu Phe His           
            580                 585                 590                   

cag gct cac gtg gcc ggg gag aca gtg gct aga cct ctg ttc ctg gaa       1824
Gln Ala His Val Ala Gly Glu Thr Val Ala Arg Pro Leu Phe Leu Glu           
        595                 600                 605                       

ttt ccc aag gac agc tcc acc tgg acc gtg gat cat cag ctg ctg tgg       1872
Phe Pro Lys Asp Ser Ser Thr Trp Thr Val Asp His Gln Leu Leu Trp           
    610                 615                 620                           

gga gaa gcc ctg ctc atc aca cct gtt ctg cag gcc gga aag gcc gaa       1920
Gly Glu Ala Leu Leu Ile Thr Pro Val Leu Gln Ala Gly Lys Ala Glu           
625                 630                 635                 640           

gtg acc ggc tat ttt cct ctc ggc act tgg tac gac ctg cag acc gtg       1968
Val Thr Gly Tyr Phe Pro Leu Gly Thr Trp Tyr Asp Leu Gln Thr Val           
                645                 650                 655               

cct att gag gcc ctg gga tct ctt cct cca cct cct gct gct cct aga       2016
Pro Ile Glu Ala Leu Gly Ser Leu Pro Pro Pro Pro Ala Ala Pro Arg           
            660                 665                 670                   

gag cct gcc atc cac tct gaa ggc cag tgg gtt aca ctg ccc gct cct       2064
Glu Pro Ala Ile His Ser Glu Gly Gln Trp Val Thr Leu Pro Ala Pro           
        675                 680                 685                       

ctg gac acc atc aac gtg cac ctg aga gct ggc tac atc atc cca ctg       2112
Leu Asp Thr Ile Asn Val His Leu Arg Ala Gly Tyr Ile Ile Pro Leu           
    690                 695                 700                           

caa ggc cct ggc ctg acc aca acc gaa tct aga cag cag ccc atg gct       2160
Gln Gly Pro Gly Leu Thr Thr Thr Glu Ser Arg Gln Gln Pro Met Ala           
705                 710                 715                 720           

ctg gcc gtg gct ctt aca aaa ggc gga gag gct aga ggc gag ctg ttc       2208
Leu Ala Val Ala Leu Thr Lys Gly Gly Glu Ala Arg Gly Glu Leu Phe           
                725                 730                 735               

tgg gac gac ggc gag tct ctg gaa gtg ctg gaa cgg ggc gct tat acc       2256
Trp Asp Asp Gly Glu Ser Leu Glu Val Leu Glu Arg Gly Ala Tyr Thr           
            740                 745                 750                   

caa gtg atc ttc ctg gcc aga aac aac acc atc gtg aac gaa ctc gtg       2304
Gln Val Ile Phe Leu Ala Arg Asn Asn Thr Ile Val Asn Glu Leu Val           
        755                 760                 765                       

cgc gtg acc agt gaa ggt gct gga ctg caa ctg cag aaa gtg acc gtg       2352
Arg Val Thr Ser Glu Gly Ala Gly Leu Gln Leu Gln Lys Val Thr Val           
    770                 775                 780                           

ctc gga gtg gcc aca gct cct caa cag gtg ctg tct aac ggc gtg ccc       2400
Leu Gly Val Ala Thr Ala Pro Gln Gln Val Leu Ser Asn Gly Val Pro           
785                 790                 795                 800           

gtg tcc aac ttc aca tac agc ccc gac acc aag gtc ctg gac atc tgt       2448
Val Ser Asn Phe Thr Tyr Ser Pro Asp Thr Lys Val Leu Asp Ile Cys           
                805                 810                 815               

gtg tca ctg ctg atg ggc gag cag ttc ctg gtg tcc tgg tgc               2490
Val Ser Leu Leu Met Gly Glu Gln Phe Leu Val Ser Trp Cys                   
            820                 825                 830                   


<210>  21
<211>  830
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  21

Gly Gln Pro Trp Cys Phe Phe Pro Pro Ser Tyr Pro Ser Tyr Lys Leu 
1               5                   10                  15      


Glu Asn Leu Ser Ser Ser Glu Met Gly Tyr Thr Ala Thr Leu Thr Arg 
            20                  25                  30          


Thr Thr Pro Thr Phe Phe Pro Lys Asp Ile Leu Thr Leu Arg Leu Asp 
        35                  40                  45              


Val Met Met Glu Thr Glu Asn Arg Leu His Phe Thr Ile Lys Asp Pro 
    50                  55                  60                  


Ala Asn Arg Arg Tyr Glu Val Pro Leu Glu Thr Pro His Val His Ser 
65                  70                  75                  80  


Arg Ala Pro Ser Pro Leu Tyr Ser Val Glu Phe Ser Glu Glu Pro Phe 
                85                  90                  95      


Gly Val Ile Val Arg Arg Gln Leu Asp Gly Arg Val Leu Leu Asn Thr 
            100                 105                 110         


Thr Val Ala Pro Leu Phe Phe Ala Asp Gln Phe Leu Gln Leu Ser Thr 
        115                 120                 125             


Ser Leu Pro Ser Gln Tyr Ile Thr Gly Leu Ala Glu His Leu Ser Pro 
    130                 135                 140                 


Leu Met Leu Ser Thr Ser Trp Thr Arg Ile Thr Leu Trp Asn Arg Asp 
145                 150                 155                 160 


Leu Ala Pro Thr Pro Gly Ala Asn Leu Tyr Gly Ser His Pro Phe Tyr 
                165                 170                 175     


Leu Ala Leu Glu Asp Gly Gly Ser Ala His Gly Val Phe Leu Leu Asn 
            180                 185                 190         


Ser Asn Ala Met Asp Val Val Leu Gln Pro Ser Pro Ala Leu Ser Trp 
        195                 200                 205             


Arg Ser Thr Gly Gly Ile Leu Asp Val Tyr Ile Phe Leu Gly Pro Glu 
    210                 215                 220                 


Pro Lys Ser Val Val Gln Gln Tyr Leu Asp Val Val Gly Tyr Pro Phe 
225                 230                 235                 240 


Met Pro Pro Tyr Trp Gly Leu Gly Phe His Leu Cys Arg Trp Gly Tyr 
                245                 250                 255     


Ser Ser Thr Ala Ile Thr Arg Gln Val Val Glu Asn Met Thr Arg Ala 
            260                 265                 270         


His Phe Pro Leu Asp Val Gln Trp Asn Asp Leu Asp Tyr Met Asp Ser 
        275                 280                 285             


Arg Arg Asp Phe Thr Phe Asn Lys Asp Gly Phe Arg Asp Phe Pro Ala 
    290                 295                 300                 


Met Val Gln Glu Leu His Gln Gly Gly Arg Arg Tyr Met Met Ile Val 
305                 310                 315                 320 


Asp Pro Ala Ile Ser Ser Ser Gly Pro Ala Gly Ser Tyr Arg Pro Tyr 
                325                 330                 335     


Asp Glu Gly Leu Arg Arg Gly Val Phe Ile Thr Asn Glu Thr Gly Gln 
            340                 345                 350         


Pro Leu Ile Gly Lys Val Trp Pro Gly Ser Thr Ala Phe Pro Asp Phe 
        355                 360                 365             


Thr Asn Pro Thr Ala Leu Ala Trp Trp Glu Asp Met Val Ala Glu Phe 
    370                 375                 380                 


His Asp Gln Val Pro Phe Asp Gly Met Trp Ile Asp Met Asn Glu Pro 
385                 390                 395                 400 


Ser Asn Phe Ile Arg Gly Ser Glu Asp Gly Cys Pro Asn Asn Glu Leu 
                405                 410                 415     


Glu Asn Pro Pro Tyr Val Pro Gly Val Val Gly Gly Thr Leu Gln Ala 
            420                 425                 430         


Ala Thr Ile Cys Ala Ser Ser His Gln Phe Leu Ser Thr His Tyr Asn 
        435                 440                 445             


Leu His Asn Leu Tyr Gly Leu Thr Glu Ala Ile Ala Ser His Arg Ala 
    450                 455                 460                 


Leu Val Lys Ala Arg Gly Thr Arg Pro Phe Val Ile Ser Arg Ser Thr 
465                 470                 475                 480 


Phe Ala Gly His Gly Arg Tyr Ala Gly His Trp Thr Gly Asp Val Trp 
                485                 490                 495     


Ser Ser Trp Glu Gln Leu Ala Ser Ser Val Pro Glu Ile Leu Gln Phe 
            500                 505                 510         


Asn Leu Leu Gly Val Pro Leu Val Gly Ala Asp Val Cys Gly Phe Leu 
        515                 520                 525             


Gly Asn Thr Ser Glu Glu Leu Cys Val Arg Trp Thr Gln Leu Gly Ala 
    530                 535                 540                 


Phe Tyr Pro Phe Met Arg Asn His Asn Ser Leu Leu Ser Leu Pro Gln 
545                 550                 555                 560 


Glu Pro Tyr Ser Phe Ser Glu Pro Ala Gln Gln Ala Met Arg Lys Ala 
                565                 570                 575     


Leu Thr Leu Arg Tyr Ala Leu Leu Pro His Leu Tyr Thr Leu Phe His 
            580                 585                 590         


Gln Ala His Val Ala Gly Glu Thr Val Ala Arg Pro Leu Phe Leu Glu 
        595                 600                 605             


Phe Pro Lys Asp Ser Ser Thr Trp Thr Val Asp His Gln Leu Leu Trp 
    610                 615                 620                 


Gly Glu Ala Leu Leu Ile Thr Pro Val Leu Gln Ala Gly Lys Ala Glu 
625                 630                 635                 640 


Val Thr Gly Tyr Phe Pro Leu Gly Thr Trp Tyr Asp Leu Gln Thr Val 
                645                 650                 655     


Pro Ile Glu Ala Leu Gly Ser Leu Pro Pro Pro Pro Ala Ala Pro Arg 
            660                 665                 670         


Glu Pro Ala Ile His Ser Glu Gly Gln Trp Val Thr Leu Pro Ala Pro 
        675                 680                 685             


Leu Asp Thr Ile Asn Val His Leu Arg Ala Gly Tyr Ile Ile Pro Leu 
    690                 695                 700                 


Gln Gly Pro Gly Leu Thr Thr Thr Glu Ser Arg Gln Gln Pro Met Ala 
705                 710                 715                 720 


Leu Ala Val Ala Leu Thr Lys Gly Gly Glu Ala Arg Gly Glu Leu Phe 
                725                 730                 735     


Trp Asp Asp Gly Glu Ser Leu Glu Val Leu Glu Arg Gly Ala Tyr Thr 
            740                 745                 750         


Gln Val Ile Phe Leu Ala Arg Asn Asn Thr Ile Val Asn Glu Leu Val 
        755                 760                 765             


Arg Val Thr Ser Glu Gly Ala Gly Leu Gln Leu Gln Lys Val Thr Val 
    770                 775                 780                 


Leu Gly Val Ala Thr Ala Pro Gln Gln Val Leu Ser Asn Gly Val Pro 
785                 790                 795                 800 


Val Ser Asn Phe Thr Tyr Ser Pro Asp Thr Lys Val Leu Asp Ile Cys 
                805                 810                 815     


Val Ser Leu Leu Met Gly Glu Gln Phe Leu Val Ser Trp Cys 
            820                 825                 830 


<210>  22
<211>  2952
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic construct


<220>
<221>  CDS
<222>  (1)..(2952)

<220>
<221>  misc_feature
<222>  (1)..(270)
<223>  BiP signal peptide + vIGF2 + 2GS extension

<220>
<221>  misc_feature
<222>  (271)..(2952)
<223>  engineered DNA for hGAA 61 - 952 780I

<220>
<221>  misc_feature
<222>  (2428)..(2430)
<223>  Ile codon

<400>  22
atg aag ctg tct ctg gtg gct gct atg ctg ctg ctc ctg tct gcc gcc         48
Met Lys Leu Ser Leu Val Ala Ala Met Leu Leu Leu Leu Ser Ala Ala           
1               5                   10                  15                

aga gcc agc aga aca ctt tgt ggc gga gag ctg gtg gac acc ctg cag         96
Arg Ala Ser Arg Thr Leu Cys Gly Gly Glu Leu Val Asp Thr Leu Gln           
            20                  25                  30                    

ttt gtg tgt ggc gac aga ggc ttc ctg ttc agc aga cct gcc agc cgg        144
Phe Val Cys Gly Asp Arg Gly Phe Leu Phe Ser Arg Pro Ala Ser Arg           
        35                  40                  45                        

gtt tcc aga cgg tct aga gga atc gtg gaa gag tgc tgc ttc aga agc        192
Val Ser Arg Arg Ser Arg Gly Ile Val Glu Glu Cys Cys Phe Arg Ser           
    50                  55                  60                            

tgc gat ctg gcc ctg ctg gaa acc tac tgt gcc aca cca gcc aga tct        240
Cys Asp Leu Ala Leu Leu Glu Thr Tyr Cys Ala Thr Pro Ala Arg Ser           
65                  70                  75                  80            

gaa ggc ggc gga gga tct ggc gga ggc gga tct aga cct gga cct aga        288
Glu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Arg Pro Gly Pro Arg           
                85                  90                  95                

gac gcc cag gct cac cct ggt aga cct aga gct gtg cct aca cag tgc        336
Asp Ala Gln Ala His Pro Gly Arg Pro Arg Ala Val Pro Thr Gln Cys           
            100                 105                 110                   

gac gtg cca cct aac agc aga ttc gac tgc gcc cct gac aag gcc atc        384
Asp Val Pro Pro Asn Ser Arg Phe Asp Cys Ala Pro Asp Lys Ala Ile           
        115                 120                 125                       

aca caa gag cag tgt gaa gcc aga ggc tgc tgc tac atc cct gcc aaa        432
Thr Gln Glu Gln Cys Glu Ala Arg Gly Cys Cys Tyr Ile Pro Ala Lys           
    130                 135                 140                           

caa gga ctg cag ggc gcc cag atg gga cag cct tgg tgc ttc ttc cca        480
Gln Gly Leu Gln Gly Ala Gln Met Gly Gln Pro Trp Cys Phe Phe Pro           
145                 150                 155                 160           

cca tct tac ccc agc tac aag ctg gaa aac ctg agc agc tcc gag atg        528
Pro Ser Tyr Pro Ser Tyr Lys Leu Glu Asn Leu Ser Ser Ser Glu Met           
                165                 170                 175               

ggc tac acc gcc aca ctg acc aga acc aca cct aca ttc ttc ccg aag        576
Gly Tyr Thr Ala Thr Leu Thr Arg Thr Thr Pro Thr Phe Phe Pro Lys           
            180                 185                 190                   

gac atc ctg aca ctg cgg ctg gac gtg atg atg gaa acc gag aac cgg        624
Asp Ile Leu Thr Leu Arg Leu Asp Val Met Met Glu Thr Glu Asn Arg           
        195                 200                 205                       

ctg cac ttc acc atc aag gac ccc gcc aat cgg aga tac gag gtg ccc        672
Leu His Phe Thr Ile Lys Asp Pro Ala Asn Arg Arg Tyr Glu Val Pro           
    210                 215                 220                           

ctg gaa aca ccc cac gtg cac tct aga gca ccc tct cca ctg tac agc        720
Leu Glu Thr Pro His Val His Ser Arg Ala Pro Ser Pro Leu Tyr Ser           
225                 230                 235                 240           

gtg gaa ttt tcc gag gaa ccc ttc ggc gtg atc gtg cgg aga cag ctg        768
Val Glu Phe Ser Glu Glu Pro Phe Gly Val Ile Val Arg Arg Gln Leu           
                245                 250                 255               

gat ggc aga gtg ctc ctg aat acc aca gtg gcc cct ctg ttc ttc gcc        816
Asp Gly Arg Val Leu Leu Asn Thr Thr Val Ala Pro Leu Phe Phe Ala           
            260                 265                 270                   

gac cag ttt ctg cag ctg agc acc agc ctg cct agc cag tat atc aca        864
Asp Gln Phe Leu Gln Leu Ser Thr Ser Leu Pro Ser Gln Tyr Ile Thr           
        275                 280                 285                       

ggc ctg gcc gag cat ctg agc cct ctg atg ctg agc aca tcc tgg acc        912
Gly Leu Ala Glu His Leu Ser Pro Leu Met Leu Ser Thr Ser Trp Thr           
    290                 295                 300                           

aga atc acc ctg tgg aac cgc gac ctg gct cct aca cct ggc gcc aat        960
Arg Ile Thr Leu Trp Asn Arg Asp Leu Ala Pro Thr Pro Gly Ala Asn           
305                 310                 315                 320           

ctg tac ggc tct cac ccc ttc tac ctg gca ctg gaa gac ggt gga tct       1008
Leu Tyr Gly Ser His Pro Phe Tyr Leu Ala Leu Glu Asp Gly Gly Ser           
                325                 330                 335               

gcc cac ggt gtc ttt ctg ctg aat agc aac gcc atg gac gtg gtg ctg       1056
Ala His Gly Val Phe Leu Leu Asn Ser Asn Ala Met Asp Val Val Leu           
            340                 345                 350                   

cag ccc tct cct gca ctg tct tgg aga tct aca ggc ggc atc ctg gac       1104
Gln Pro Ser Pro Ala Leu Ser Trp Arg Ser Thr Gly Gly Ile Leu Asp           
        355                 360                 365                       

gtg tac atc ttt ctg ggc ccc gag cct aag agc gtg gtg cag cag tat       1152
Val Tyr Ile Phe Leu Gly Pro Glu Pro Lys Ser Val Val Gln Gln Tyr           
    370                 375                 380                           

ctg gac gtc gtg ggc tac ccc ttc atg cct cct tat tgg ggc ctg ggc       1200
Leu Asp Val Val Gly Tyr Pro Phe Met Pro Pro Tyr Trp Gly Leu Gly           
385                 390                 395                 400           

ttc cac ctg tgt agg tgg ggc tac agc agc acc gcc atc acc aga cag       1248
Phe His Leu Cys Arg Trp Gly Tyr Ser Ser Thr Ala Ile Thr Arg Gln           
                405                 410                 415               

gtg gtg gaa aac atg acc cgg gct cac ttc cca ctg gac gtg cag tgg       1296
Val Val Glu Asn Met Thr Arg Ala His Phe Pro Leu Asp Val Gln Trp           
            420                 425                 430                   

aac gac ctg gac tac atg gac agc aga cgg gac ttc acc ttc aac aag       1344
Asn Asp Leu Asp Tyr Met Asp Ser Arg Arg Asp Phe Thr Phe Asn Lys           
        435                 440                 445                       

gac ggc ttc aga gac ttc ccc gcc atg gtg caa gag ctg cat caa ggc       1392
Asp Gly Phe Arg Asp Phe Pro Ala Met Val Gln Glu Leu His Gln Gly           
    450                 455                 460                           

gga cgg cgg tac atg atg att gtg gac cct gcc atc agc agc tct gga       1440
Gly Arg Arg Tyr Met Met Ile Val Asp Pro Ala Ile Ser Ser Ser Gly           
465                 470                 475                 480           

cca gcc ggc agc tac aga cct tac gat gag gga ctg aga aga ggc gtg       1488
Pro Ala Gly Ser Tyr Arg Pro Tyr Asp Glu Gly Leu Arg Arg Gly Val           
                485                 490                 495               

ttc atc acc aac gag aca ggc cag cct ctg atc ggc aaa gtg tgg cct       1536
Phe Ile Thr Asn Glu Thr Gly Gln Pro Leu Ile Gly Lys Val Trp Pro           
            500                 505                 510                   

ggc agc aca gcc ttt cca gac ttc aca aac ccc acc gct ctg gct tgg       1584
Gly Ser Thr Ala Phe Pro Asp Phe Thr Asn Pro Thr Ala Leu Ala Trp           
        515                 520                 525                       

tgg gaa gat atg gtg gcc gag ttc cac gat cag gtg ccc ttc gat ggc       1632
Trp Glu Asp Met Val Ala Glu Phe His Asp Gln Val Pro Phe Asp Gly           
    530                 535                 540                           

atg tgg atc gac atg aac gag ccc agc aac ttc atc cgg ggc agc gag       1680
Met Trp Ile Asp Met Asn Glu Pro Ser Asn Phe Ile Arg Gly Ser Glu           
545                 550                 555                 560           

gat ggc tgc ccc aac aac gaa cta gaa aat cct cct tac gtg ccc ggc       1728
Asp Gly Cys Pro Asn Asn Glu Leu Glu Asn Pro Pro Tyr Val Pro Gly           
                565                 570                 575               

gtt gtc ggc gga aca ctt cag gcc gct aca atc tgt gcc agc agc cat       1776
Val Val Gly Gly Thr Leu Gln Ala Ala Thr Ile Cys Ala Ser Ser His           
            580                 585                 590                   

cag ttt ctg agc acc cac tac aac ctg cac aac ctg tac ggc ctg acc       1824
Gln Phe Leu Ser Thr His Tyr Asn Leu His Asn Leu Tyr Gly Leu Thr           
        595                 600                 605                       

gag gcc att gcc tct cat aga gcc ctg gtt aag gcc aga ggc acc cgg       1872
Glu Ala Ile Ala Ser His Arg Ala Leu Val Lys Ala Arg Gly Thr Arg           
    610                 615                 620                           

cct ttt gtg atc agc aga agc aca ttc gcc ggc cac ggc aga tac gca       1920
Pro Phe Val Ile Ser Arg Ser Thr Phe Ala Gly His Gly Arg Tyr Ala           
625                 630                 635                 640           

gga cat tgg aca ggc gac gtg tgg tct agt tgg gag cag ctg gct agc       1968
Gly His Trp Thr Gly Asp Val Trp Ser Ser Trp Glu Gln Leu Ala Ser           
                645                 650                 655               

agc gtg cca gag atc ctg cag ttc aat ctg ctg ggc gtg cca ctc gtg       2016
Ser Val Pro Glu Ile Leu Gln Phe Asn Leu Leu Gly Val Pro Leu Val           
            660                 665                 670                   

gga gcc gac gtt tgt ggc ttc ctg ggc aac acc agc gag gaa ctg tgt       2064
Gly Ala Asp Val Cys Gly Phe Leu Gly Asn Thr Ser Glu Glu Leu Cys           
        675                 680                 685                       

gtg cgt tgg aca cag ctg ggc gcc ttc tat ccc ttc atg aga aac cac       2112
Val Arg Trp Thr Gln Leu Gly Ala Phe Tyr Pro Phe Met Arg Asn His           
    690                 695                 700                           

aac agc ctg ctg agc ctg cct caa gag ccc tac agc ttt agc gag cct       2160
Asn Ser Leu Leu Ser Leu Pro Gln Glu Pro Tyr Ser Phe Ser Glu Pro           
705                 710                 715                 720           

gca cag cag gcc atg aga aag gcc ctg act ctg aga tac gcc ctg ctg       2208
Ala Gln Gln Ala Met Arg Lys Ala Leu Thr Leu Arg Tyr Ala Leu Leu           
                725                 730                 735               

cct cac ctg tac acc ctg ttt cat cag gcc cac gtg gca ggc gag aca       2256
Pro His Leu Tyr Thr Leu Phe His Gln Ala His Val Ala Gly Glu Thr           
            740                 745                 750                   

gtg gct aga cct ctg ttc ctg gaa ttt ccc aag gac agc tcc acc tgg       2304
Val Ala Arg Pro Leu Phe Leu Glu Phe Pro Lys Asp Ser Ser Thr Trp           
        755                 760                 765                       

acc gtg gat cat cag ctg ctg tgg gga gaa gcc ctg ctg att aca cca       2352
Thr Val Asp His Gln Leu Leu Trp Gly Glu Ala Leu Leu Ile Thr Pro           
    770                 775                 780                           

gtg ctg cag gcc gga aag gcc gaa gtg aca ggc tat ttc cct ctc ggc       2400
Val Leu Gln Ala Gly Lys Ala Glu Val Thr Gly Tyr Phe Pro Leu Gly           
785                 790                 795                 800           

act tgg tac gac ctg cag acc gtg cct atc gag gct ctg gga tct ctt       2448
Thr Trp Tyr Asp Leu Gln Thr Val Pro Ile Glu Ala Leu Gly Ser Leu           
                805                 810                 815               

cct cca cct cct gcc gct cct aga gag cct gcc att cac tct gaa ggc       2496
Pro Pro Pro Pro Ala Ala Pro Arg Glu Pro Ala Ile His Ser Glu Gly           
            820                 825                 830                   

cag tgg gtt acc ctg cct gct cct ctg gac acc atc aac gtg cac ctg       2544
Gln Trp Val Thr Leu Pro Ala Pro Leu Asp Thr Ile Asn Val His Leu           
        835                 840                 845                       

aga gcc ggc tac atc atc cct ctg caa ggc cct ggc ctg acc aca acc       2592
Arg Ala Gly Tyr Ile Ile Pro Leu Gln Gly Pro Gly Leu Thr Thr Thr           
    850                 855                 860                           

gaa tct aga cag cag ccc atg gca ctg gcc gtg gct ctt aca aaa ggc       2640
Glu Ser Arg Gln Gln Pro Met Ala Leu Ala Val Ala Leu Thr Lys Gly           
865                 870                 875                 880           

gga gag gct aga ggc gag ctg ttc tgg gat gat ggc gag agc cta gaa       2688
Gly Glu Ala Arg Gly Glu Leu Phe Trp Asp Asp Gly Glu Ser Leu Glu           
                885                 890                 895               

gtg ctg gaa cgg ggc gct tat acc caa gtg atc ttc ctg gcc aga aac       2736
Val Leu Glu Arg Gly Ala Tyr Thr Gln Val Ile Phe Leu Ala Arg Asn           
            900                 905                 910                   

aac acc atc gtg aac gaa ctc gtg cgc gtg acc agt gaa ggt gct gga       2784
Asn Thr Ile Val Asn Glu Leu Val Arg Val Thr Ser Glu Gly Ala Gly           
        915                 920                 925                       

ctg caa ctg cag aaa gtg acc gtg ctc gga gtg gcc aca gct cct cag       2832
Leu Gln Leu Gln Lys Val Thr Val Leu Gly Val Ala Thr Ala Pro Gln           
    930                 935                 940                           

cag gtt ctg tct aat ggc gtg ccc gtg tcc aac ttc aca tac agc ccc       2880
Gln Val Leu Ser Asn Gly Val Pro Val Ser Asn Phe Thr Tyr Ser Pro           
945                 950                 955                 960           

gac acc aag gtc ctg gac atc tgt gtg tcc ctg ctt atg ggc gag cag       2928
Asp Thr Lys Val Leu Asp Ile Cys Val Ser Leu Leu Met Gly Glu Gln           
                965                 970                 975               

ttc ctg gtg tcc tgg tgc tga taa                                       2952
Phe Leu Val Ser Trp Cys                                                   
            980                                                           


<210>  23
<211>  982
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  23

Met Lys Leu Ser Leu Val Ala Ala Met Leu Leu Leu Leu Ser Ala Ala 
1               5                   10                  15      


Arg Ala Ser Arg Thr Leu Cys Gly Gly Glu Leu Val Asp Thr Leu Gln 
            20                  25                  30          


Phe Val Cys Gly Asp Arg Gly Phe Leu Phe Ser Arg Pro Ala Ser Arg 
        35                  40                  45              


Val Ser Arg Arg Ser Arg Gly Ile Val Glu Glu Cys Cys Phe Arg Ser 
    50                  55                  60                  


Cys Asp Leu Ala Leu Leu Glu Thr Tyr Cys Ala Thr Pro Ala Arg Ser 
65                  70                  75                  80  


Glu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Arg Pro Gly Pro Arg 
                85                  90                  95      


Asp Ala Gln Ala His Pro Gly Arg Pro Arg Ala Val Pro Thr Gln Cys 
            100                 105                 110         


Asp Val Pro Pro Asn Ser Arg Phe Asp Cys Ala Pro Asp Lys Ala Ile 
        115                 120                 125             


Thr Gln Glu Gln Cys Glu Ala Arg Gly Cys Cys Tyr Ile Pro Ala Lys 
    130                 135                 140                 


Gln Gly Leu Gln Gly Ala Gln Met Gly Gln Pro Trp Cys Phe Phe Pro 
145                 150                 155                 160 


Pro Ser Tyr Pro Ser Tyr Lys Leu Glu Asn Leu Ser Ser Ser Glu Met 
                165                 170                 175     


Gly Tyr Thr Ala Thr Leu Thr Arg Thr Thr Pro Thr Phe Phe Pro Lys 
            180                 185                 190         


Asp Ile Leu Thr Leu Arg Leu Asp Val Met Met Glu Thr Glu Asn Arg 
        195                 200                 205             


Leu His Phe Thr Ile Lys Asp Pro Ala Asn Arg Arg Tyr Glu Val Pro 
    210                 215                 220                 


Leu Glu Thr Pro His Val His Ser Arg Ala Pro Ser Pro Leu Tyr Ser 
225                 230                 235                 240 


Val Glu Phe Ser Glu Glu Pro Phe Gly Val Ile Val Arg Arg Gln Leu 
                245                 250                 255     


Asp Gly Arg Val Leu Leu Asn Thr Thr Val Ala Pro Leu Phe Phe Ala 
            260                 265                 270         


Asp Gln Phe Leu Gln Leu Ser Thr Ser Leu Pro Ser Gln Tyr Ile Thr 
        275                 280                 285             


Gly Leu Ala Glu His Leu Ser Pro Leu Met Leu Ser Thr Ser Trp Thr 
    290                 295                 300                 


Arg Ile Thr Leu Trp Asn Arg Asp Leu Ala Pro Thr Pro Gly Ala Asn 
305                 310                 315                 320 


Leu Tyr Gly Ser His Pro Phe Tyr Leu Ala Leu Glu Asp Gly Gly Ser 
                325                 330                 335     


Ala His Gly Val Phe Leu Leu Asn Ser Asn Ala Met Asp Val Val Leu 
            340                 345                 350         


Gln Pro Ser Pro Ala Leu Ser Trp Arg Ser Thr Gly Gly Ile Leu Asp 
        355                 360                 365             


Val Tyr Ile Phe Leu Gly Pro Glu Pro Lys Ser Val Val Gln Gln Tyr 
    370                 375                 380                 


Leu Asp Val Val Gly Tyr Pro Phe Met Pro Pro Tyr Trp Gly Leu Gly 
385                 390                 395                 400 


Phe His Leu Cys Arg Trp Gly Tyr Ser Ser Thr Ala Ile Thr Arg Gln 
                405                 410                 415     


Val Val Glu Asn Met Thr Arg Ala His Phe Pro Leu Asp Val Gln Trp 
            420                 425                 430         


Asn Asp Leu Asp Tyr Met Asp Ser Arg Arg Asp Phe Thr Phe Asn Lys 
        435                 440                 445             


Asp Gly Phe Arg Asp Phe Pro Ala Met Val Gln Glu Leu His Gln Gly 
    450                 455                 460                 


Gly Arg Arg Tyr Met Met Ile Val Asp Pro Ala Ile Ser Ser Ser Gly 
465                 470                 475                 480 


Pro Ala Gly Ser Tyr Arg Pro Tyr Asp Glu Gly Leu Arg Arg Gly Val 
                485                 490                 495     


Phe Ile Thr Asn Glu Thr Gly Gln Pro Leu Ile Gly Lys Val Trp Pro 
            500                 505                 510         


Gly Ser Thr Ala Phe Pro Asp Phe Thr Asn Pro Thr Ala Leu Ala Trp 
        515                 520                 525             


Trp Glu Asp Met Val Ala Glu Phe His Asp Gln Val Pro Phe Asp Gly 
    530                 535                 540                 


Met Trp Ile Asp Met Asn Glu Pro Ser Asn Phe Ile Arg Gly Ser Glu 
545                 550                 555                 560 


Asp Gly Cys Pro Asn Asn Glu Leu Glu Asn Pro Pro Tyr Val Pro Gly 
                565                 570                 575     


Val Val Gly Gly Thr Leu Gln Ala Ala Thr Ile Cys Ala Ser Ser His 
            580                 585                 590         


Gln Phe Leu Ser Thr His Tyr Asn Leu His Asn Leu Tyr Gly Leu Thr 
        595                 600                 605             


Glu Ala Ile Ala Ser His Arg Ala Leu Val Lys Ala Arg Gly Thr Arg 
    610                 615                 620                 


Pro Phe Val Ile Ser Arg Ser Thr Phe Ala Gly His Gly Arg Tyr Ala 
625                 630                 635                 640 


Gly His Trp Thr Gly Asp Val Trp Ser Ser Trp Glu Gln Leu Ala Ser 
                645                 650                 655     


Ser Val Pro Glu Ile Leu Gln Phe Asn Leu Leu Gly Val Pro Leu Val 
            660                 665                 670         


Gly Ala Asp Val Cys Gly Phe Leu Gly Asn Thr Ser Glu Glu Leu Cys 
        675                 680                 685             


Val Arg Trp Thr Gln Leu Gly Ala Phe Tyr Pro Phe Met Arg Asn His 
    690                 695                 700                 


Asn Ser Leu Leu Ser Leu Pro Gln Glu Pro Tyr Ser Phe Ser Glu Pro 
705                 710                 715                 720 


Ala Gln Gln Ala Met Arg Lys Ala Leu Thr Leu Arg Tyr Ala Leu Leu 
                725                 730                 735     


Pro His Leu Tyr Thr Leu Phe His Gln Ala His Val Ala Gly Glu Thr 
            740                 745                 750         


Val Ala Arg Pro Leu Phe Leu Glu Phe Pro Lys Asp Ser Ser Thr Trp 
        755                 760                 765             


Thr Val Asp His Gln Leu Leu Trp Gly Glu Ala Leu Leu Ile Thr Pro 
    770                 775                 780                 


Val Leu Gln Ala Gly Lys Ala Glu Val Thr Gly Tyr Phe Pro Leu Gly 
785                 790                 795                 800 


Thr Trp Tyr Asp Leu Gln Thr Val Pro Ile Glu Ala Leu Gly Ser Leu 
                805                 810                 815     


Pro Pro Pro Pro Ala Ala Pro Arg Glu Pro Ala Ile His Ser Glu Gly 
            820                 825                 830         


Gln Trp Val Thr Leu Pro Ala Pro Leu Asp Thr Ile Asn Val His Leu 
        835                 840                 845             


Arg Ala Gly Tyr Ile Ile Pro Leu Gln Gly Pro Gly Leu Thr Thr Thr 
    850                 855                 860                 


Glu Ser Arg Gln Gln Pro Met Ala Leu Ala Val Ala Leu Thr Lys Gly 
865                 870                 875                 880 


Gly Glu Ala Arg Gly Glu Leu Phe Trp Asp Asp Gly Glu Ser Leu Glu 
                885                 890                 895     


Val Leu Glu Arg Gly Ala Tyr Thr Gln Val Ile Phe Leu Ala Arg Asn 
            900                 905                 910         


Asn Thr Ile Val Asn Glu Leu Val Arg Val Thr Ser Glu Gly Ala Gly 
        915                 920                 925             


Leu Gln Leu Gln Lys Val Thr Val Leu Gly Val Ala Thr Ala Pro Gln 
    930                 935                 940                 


Gln Val Leu Ser Asn Gly Val Pro Val Ser Asn Phe Thr Tyr Ser Pro 
945                 950                 955                 960 


Asp Thr Lys Val Leu Asp Ile Cys Val Ser Leu Leu Met Gly Glu Gln 
                965                 970                 975     


Phe Leu Val Ser Trp Cys 
            980         


<210>  24
<211>  2952
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic construct


<220>
<221>  CDS
<222>  (1)..(2952)

<220>
<221>  misc_feature
<222>  (1)..(270)
<223>  BiP-vIGF peptide

<220>
<221>  misc_feature
<222>  (1)..(270)
<223>  BiP signal peptide + vIGF2+2GS extension

<220>
<221>  misc_feature
<222>  (271)..(2952)
<223>  hGAA 61-952 V780 DNA

<220>
<221>  misc_feature
<222>  (2428)..(2430)
<223>  codon for hGAA 780 Valine

<400>  24
atg aag ctg tct ctg gtg gct gct atg ctg ctg ctc ctg tct gcc gcc         48
Met Lys Leu Ser Leu Val Ala Ala Met Leu Leu Leu Leu Ser Ala Ala           
1               5                   10                  15                

aga gcc agc aga aca ctt tgt ggc gga gag ctg gtg gac acc ctg cag         96
Arg Ala Ser Arg Thr Leu Cys Gly Gly Glu Leu Val Asp Thr Leu Gln           
            20                  25                  30                    

ttt gtg tgt ggc gac aga ggc ttc ctg ttc agc aga cct gcc agc cgg        144
Phe Val Cys Gly Asp Arg Gly Phe Leu Phe Ser Arg Pro Ala Ser Arg           
        35                  40                  45                        

gtt tcc aga cgg tct aga gga atc gtg gaa gag tgc tgc ttc aga agc        192
Val Ser Arg Arg Ser Arg Gly Ile Val Glu Glu Cys Cys Phe Arg Ser           
    50                  55                  60                            

tgc gat ctg gcc ctg ctg gaa acc tac tgt gcc aca cca gcc aga tct        240
Cys Asp Leu Ala Leu Leu Glu Thr Tyr Cys Ala Thr Pro Ala Arg Ser           
65                  70                  75                  80            

gaa ggc ggc gga gga tct ggc gga ggc gga tct aga cct gga cct aga        288
Glu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Arg Pro Gly Pro Arg           
                85                  90                  95                

gac gcc cag gct cac cct ggt aga cct aga gct gtg cct aca cag tgc        336
Asp Ala Gln Ala His Pro Gly Arg Pro Arg Ala Val Pro Thr Gln Cys           
            100                 105                 110                   

gac gtg cca cct aac agc aga ttc gac tgc gcc cct gac aag gcc atc        384
Asp Val Pro Pro Asn Ser Arg Phe Asp Cys Ala Pro Asp Lys Ala Ile           
        115                 120                 125                       

aca caa gag cag tgt gaa gcc aga ggc tgc tgc tac atc cct gcc aaa        432
Thr Gln Glu Gln Cys Glu Ala Arg Gly Cys Cys Tyr Ile Pro Ala Lys           
    130                 135                 140                           

caa gga ctg cag ggc gcc cag atg gga cag cct tgg tgc ttc ttc cca        480
Gln Gly Leu Gln Gly Ala Gln Met Gly Gln Pro Trp Cys Phe Phe Pro           
145                 150                 155                 160           

cca tct tac ccc agc tac aag ctg gaa aac ctg agc agc tcc gag atg        528
Pro Ser Tyr Pro Ser Tyr Lys Leu Glu Asn Leu Ser Ser Ser Glu Met           
                165                 170                 175               

ggc tac acc gcc aca ctg acc aga acc aca cct aca ttc ttc ccg aag        576
Gly Tyr Thr Ala Thr Leu Thr Arg Thr Thr Pro Thr Phe Phe Pro Lys           
            180                 185                 190                   

gac atc ctg aca ctg cgg ctg gac gtg atg atg gaa acc gag aac cgg        624
Asp Ile Leu Thr Leu Arg Leu Asp Val Met Met Glu Thr Glu Asn Arg           
        195                 200                 205                       

ctg cac ttc acc atc aag gac ccc gcc aat cgg aga tac gag gtg ccc        672
Leu His Phe Thr Ile Lys Asp Pro Ala Asn Arg Arg Tyr Glu Val Pro           
    210                 215                 220                           

ctg gaa aca ccc cac gtg cac tct aga gca ccc tct cca ctg tac agc        720
Leu Glu Thr Pro His Val His Ser Arg Ala Pro Ser Pro Leu Tyr Ser           
225                 230                 235                 240           

gtg gaa ttt tcc gag gaa ccc ttc ggc gtg atc gtg cgg aga cag ctg        768
Val Glu Phe Ser Glu Glu Pro Phe Gly Val Ile Val Arg Arg Gln Leu           
                245                 250                 255               

gat ggc aga gtg ctc ctg aat acc aca gtg gcc cct ctg ttc ttc gcc        816
Asp Gly Arg Val Leu Leu Asn Thr Thr Val Ala Pro Leu Phe Phe Ala           
            260                 265                 270                   

gac cag ttt ctg cag ctg agc acc agc ctg cct agc cag tat atc aca        864
Asp Gln Phe Leu Gln Leu Ser Thr Ser Leu Pro Ser Gln Tyr Ile Thr           
        275                 280                 285                       

ggc ctg gcc gag cat ctg agc cct ctg atg ctg agc aca tcc tgg acc        912
Gly Leu Ala Glu His Leu Ser Pro Leu Met Leu Ser Thr Ser Trp Thr           
    290                 295                 300                           

aga atc acc ctg tgg aac cgc gac ctg gct cct aca cct ggc gcc aat        960
Arg Ile Thr Leu Trp Asn Arg Asp Leu Ala Pro Thr Pro Gly Ala Asn           
305                 310                 315                 320           

ctg tac ggc tct cac ccc ttc tac ctg gca ctg gaa gac ggt gga tct       1008
Leu Tyr Gly Ser His Pro Phe Tyr Leu Ala Leu Glu Asp Gly Gly Ser           
                325                 330                 335               

gcc cac ggt gtc ttt ctg ctg aat agc aac gcc atg gac gtg gtg ctg       1056
Ala His Gly Val Phe Leu Leu Asn Ser Asn Ala Met Asp Val Val Leu           
            340                 345                 350                   

cag ccc tct cct gca ctg tct tgg aga tct aca ggc ggc atc ctg gac       1104
Gln Pro Ser Pro Ala Leu Ser Trp Arg Ser Thr Gly Gly Ile Leu Asp           
        355                 360                 365                       

gtg tac atc ttt ctg ggc ccc gag cct aag agc gtg gtg cag cag tat       1152
Val Tyr Ile Phe Leu Gly Pro Glu Pro Lys Ser Val Val Gln Gln Tyr           
    370                 375                 380                           

ctg gac gtc gtg ggc tac ccc ttc atg cct cct tat tgg ggc ctg ggc       1200
Leu Asp Val Val Gly Tyr Pro Phe Met Pro Pro Tyr Trp Gly Leu Gly           
385                 390                 395                 400           

ttc cac ctg tgt agg tgg ggc tac agc agc acc gcc atc acc aga cag       1248
Phe His Leu Cys Arg Trp Gly Tyr Ser Ser Thr Ala Ile Thr Arg Gln           
                405                 410                 415               

gtg gtg gaa aac atg acc cgg gct cac ttc cca ctg gac gtg cag tgg       1296
Val Val Glu Asn Met Thr Arg Ala His Phe Pro Leu Asp Val Gln Trp           
            420                 425                 430                   

aac gac ctg gac tac atg gac agc aga cgg gac ttc acc ttc aac aag       1344
Asn Asp Leu Asp Tyr Met Asp Ser Arg Arg Asp Phe Thr Phe Asn Lys           
        435                 440                 445                       

gac ggc ttc aga gac ttc ccc gcc atg gtg caa gag ctg cat caa ggc       1392
Asp Gly Phe Arg Asp Phe Pro Ala Met Val Gln Glu Leu His Gln Gly           
    450                 455                 460                           

gga cgg cgg tac atg atg att gtg gac cct gcc atc agc agc tct gga       1440
Gly Arg Arg Tyr Met Met Ile Val Asp Pro Ala Ile Ser Ser Ser Gly           
465                 470                 475                 480           

cca gcc ggc agc tac aga cct tac gat gag gga ctg aga aga ggc gtg       1488
Pro Ala Gly Ser Tyr Arg Pro Tyr Asp Glu Gly Leu Arg Arg Gly Val           
                485                 490                 495               

ttc atc acc aac gag aca ggc cag cct ctg atc ggc aaa gtg tgg cct       1536
Phe Ile Thr Asn Glu Thr Gly Gln Pro Leu Ile Gly Lys Val Trp Pro           
            500                 505                 510                   

ggc agc aca gcc ttt cca gac ttc aca aac ccc acc gct ctg gct tgg       1584
Gly Ser Thr Ala Phe Pro Asp Phe Thr Asn Pro Thr Ala Leu Ala Trp           
        515                 520                 525                       

tgg gaa gat atg gtg gcc gag ttc cac gat cag gtg ccc ttc gat ggc       1632
Trp Glu Asp Met Val Ala Glu Phe His Asp Gln Val Pro Phe Asp Gly           
    530                 535                 540                           

atg tgg atc gac atg aac gag ccc agc aac ttc atc cgg ggc agc gag       1680
Met Trp Ile Asp Met Asn Glu Pro Ser Asn Phe Ile Arg Gly Ser Glu           
545                 550                 555                 560           

gat ggc tgc ccc aac aac gaa cta gaa aat cct cct tac gtg ccc ggc       1728
Asp Gly Cys Pro Asn Asn Glu Leu Glu Asn Pro Pro Tyr Val Pro Gly           
                565                 570                 575               

gtt gtc ggc gga aca ctt cag gcc gct aca atc tgt gcc agc agc cat       1776
Val Val Gly Gly Thr Leu Gln Ala Ala Thr Ile Cys Ala Ser Ser His           
            580                 585                 590                   

cag ttt ctg agc acc cac tac aac ctg cac aac ctg tac ggc ctg acc       1824
Gln Phe Leu Ser Thr His Tyr Asn Leu His Asn Leu Tyr Gly Leu Thr           
        595                 600                 605                       

gag gcc att gcc tct cat aga gcc ctg gtt aag gcc aga ggc acc cgg       1872
Glu Ala Ile Ala Ser His Arg Ala Leu Val Lys Ala Arg Gly Thr Arg           
    610                 615                 620                           

cct ttt gtg atc agc aga agc aca ttc gcc ggc cac ggc aga tac gca       1920
Pro Phe Val Ile Ser Arg Ser Thr Phe Ala Gly His Gly Arg Tyr Ala           
625                 630                 635                 640           

gga cat tgg aca ggc gac gtg tgg tct agt tgg gag cag ctg gct agc       1968
Gly His Trp Thr Gly Asp Val Trp Ser Ser Trp Glu Gln Leu Ala Ser           
                645                 650                 655               

agc gtg cca gag atc ctg cag ttc aat ctg ctg ggc gtg cca ctc gtg       2016
Ser Val Pro Glu Ile Leu Gln Phe Asn Leu Leu Gly Val Pro Leu Val           
            660                 665                 670                   

gga gcc gac gtt tgt ggc ttc ctg ggc aac acc agc gag gaa ctg tgt       2064
Gly Ala Asp Val Cys Gly Phe Leu Gly Asn Thr Ser Glu Glu Leu Cys           
        675                 680                 685                       

gtg cgt tgg aca cag ctg ggc gcc ttc tat ccc ttc atg aga aac cac       2112
Val Arg Trp Thr Gln Leu Gly Ala Phe Tyr Pro Phe Met Arg Asn His           
    690                 695                 700                           

aac agc ctg ctg agc ctg cct caa gag ccc tac agc ttt agc gag cct       2160
Asn Ser Leu Leu Ser Leu Pro Gln Glu Pro Tyr Ser Phe Ser Glu Pro           
705                 710                 715                 720           

gca cag cag gcc atg aga aag gcc ctg act ctg aga tac gcc ctg ctg       2208
Ala Gln Gln Ala Met Arg Lys Ala Leu Thr Leu Arg Tyr Ala Leu Leu           
                725                 730                 735               

cct cac ctg tac acc ctg ttt cat cag gcc cac gtg gca ggc gag aca       2256
Pro His Leu Tyr Thr Leu Phe His Gln Ala His Val Ala Gly Glu Thr           
            740                 745                 750                   

gtg gct aga cct ctg ttc ctg gaa ttt ccc aag gac agc tcc acc tgg       2304
Val Ala Arg Pro Leu Phe Leu Glu Phe Pro Lys Asp Ser Ser Thr Trp           
        755                 760                 765                       

acc gtg gat cat cag ctg ctg tgg gga gaa gcc ctg ctg att aca cca       2352
Thr Val Asp His Gln Leu Leu Trp Gly Glu Ala Leu Leu Ile Thr Pro           
    770                 775                 780                           

gtg ctg cag gcc gga aag gcc gaa gtg aca ggc tat ttc cct ctc ggc       2400
Val Leu Gln Ala Gly Lys Ala Glu Val Thr Gly Tyr Phe Pro Leu Gly           
785                 790                 795                 800           

act tgg tac gac ctg cag acc gtg cct atc gag gct ctg gga tct ctt       2448
Thr Trp Tyr Asp Leu Gln Thr Val Pro Ile Glu Ala Leu Gly Ser Leu           
                805                 810                 815               

cct cca cct cct gcc gct cct aga gag cct gcc att cac tct gaa ggc       2496
Pro Pro Pro Pro Ala Ala Pro Arg Glu Pro Ala Ile His Ser Glu Gly           
            820                 825                 830                   

cag tgg gtt acc ctg cct gct cct ctg gac acc atc aac gtg cac ctg       2544
Gln Trp Val Thr Leu Pro Ala Pro Leu Asp Thr Ile Asn Val His Leu           
        835                 840                 845                       

aga gcc ggc tac atc atc cct ctg caa ggc cct ggc ctg acc aca acc       2592
Arg Ala Gly Tyr Ile Ile Pro Leu Gln Gly Pro Gly Leu Thr Thr Thr           
    850                 855                 860                           

gaa tct aga cag cag ccc atg gca ctg gcc gtg gct ctt aca aaa ggc       2640
Glu Ser Arg Gln Gln Pro Met Ala Leu Ala Val Ala Leu Thr Lys Gly           
865                 870                 875                 880           

gga gag gct aga ggc gag ctg ttc tgg gat gat ggc gag agc cta gaa       2688
Gly Glu Ala Arg Gly Glu Leu Phe Trp Asp Asp Gly Glu Ser Leu Glu           
                885                 890                 895               

gtg ctg gaa cgg ggc gct tat acc caa gtg atc ttc ctg gcc aga aac       2736
Val Leu Glu Arg Gly Ala Tyr Thr Gln Val Ile Phe Leu Ala Arg Asn           
            900                 905                 910                   

aac acc atc gtg aac gaa ctc gtg cgc gtg acc agt gaa ggt gct gga       2784
Asn Thr Ile Val Asn Glu Leu Val Arg Val Thr Ser Glu Gly Ala Gly           
        915                 920                 925                       

ctg caa ctg cag aaa gtg acc gtg ctc gga gtg gcc aca gct cct cag       2832
Leu Gln Leu Gln Lys Val Thr Val Leu Gly Val Ala Thr Ala Pro Gln           
    930                 935                 940                           

cag gtt ctg tct aat ggc gtg ccc gtg tcc aac ttc aca tac agc ccc       2880
Gln Val Leu Ser Asn Gly Val Pro Val Ser Asn Phe Thr Tyr Ser Pro           
945                 950                 955                 960           

gac acc aag gtc ctg gac atc tgt gtg tcc ctg ctt atg ggc gag cag       2928
Asp Thr Lys Val Leu Asp Ile Cys Val Ser Leu Leu Met Gly Glu Gln           
                965                 970                 975               

ttc ctg gtg tcc tgg tgc tga taa                                       2952
Phe Leu Val Ser Trp Cys                                                   
            980                                                           


<210>  25
<211>  982
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  25

Met Lys Leu Ser Leu Val Ala Ala Met Leu Leu Leu Leu Ser Ala Ala 
1               5                   10                  15      


Arg Ala Ser Arg Thr Leu Cys Gly Gly Glu Leu Val Asp Thr Leu Gln 
            20                  25                  30          


Phe Val Cys Gly Asp Arg Gly Phe Leu Phe Ser Arg Pro Ala Ser Arg 
        35                  40                  45              


Val Ser Arg Arg Ser Arg Gly Ile Val Glu Glu Cys Cys Phe Arg Ser 
    50                  55                  60                  


Cys Asp Leu Ala Leu Leu Glu Thr Tyr Cys Ala Thr Pro Ala Arg Ser 
65                  70                  75                  80  


Glu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Arg Pro Gly Pro Arg 
                85                  90                  95      


Asp Ala Gln Ala His Pro Gly Arg Pro Arg Ala Val Pro Thr Gln Cys 
            100                 105                 110         


Asp Val Pro Pro Asn Ser Arg Phe Asp Cys Ala Pro Asp Lys Ala Ile 
        115                 120                 125             


Thr Gln Glu Gln Cys Glu Ala Arg Gly Cys Cys Tyr Ile Pro Ala Lys 
    130                 135                 140                 


Gln Gly Leu Gln Gly Ala Gln Met Gly Gln Pro Trp Cys Phe Phe Pro 
145                 150                 155                 160 


Pro Ser Tyr Pro Ser Tyr Lys Leu Glu Asn Leu Ser Ser Ser Glu Met 
                165                 170                 175     


Gly Tyr Thr Ala Thr Leu Thr Arg Thr Thr Pro Thr Phe Phe Pro Lys 
            180                 185                 190         


Asp Ile Leu Thr Leu Arg Leu Asp Val Met Met Glu Thr Glu Asn Arg 
        195                 200                 205             


Leu His Phe Thr Ile Lys Asp Pro Ala Asn Arg Arg Tyr Glu Val Pro 
    210                 215                 220                 


Leu Glu Thr Pro His Val His Ser Arg Ala Pro Ser Pro Leu Tyr Ser 
225                 230                 235                 240 


Val Glu Phe Ser Glu Glu Pro Phe Gly Val Ile Val Arg Arg Gln Leu 
                245                 250                 255     


Asp Gly Arg Val Leu Leu Asn Thr Thr Val Ala Pro Leu Phe Phe Ala 
            260                 265                 270         


Asp Gln Phe Leu Gln Leu Ser Thr Ser Leu Pro Ser Gln Tyr Ile Thr 
        275                 280                 285             


Gly Leu Ala Glu His Leu Ser Pro Leu Met Leu Ser Thr Ser Trp Thr 
    290                 295                 300                 


Arg Ile Thr Leu Trp Asn Arg Asp Leu Ala Pro Thr Pro Gly Ala Asn 
305                 310                 315                 320 


Leu Tyr Gly Ser His Pro Phe Tyr Leu Ala Leu Glu Asp Gly Gly Ser 
                325                 330                 335     


Ala His Gly Val Phe Leu Leu Asn Ser Asn Ala Met Asp Val Val Leu 
            340                 345                 350         


Gln Pro Ser Pro Ala Leu Ser Trp Arg Ser Thr Gly Gly Ile Leu Asp 
        355                 360                 365             


Val Tyr Ile Phe Leu Gly Pro Glu Pro Lys Ser Val Val Gln Gln Tyr 
    370                 375                 380                 


Leu Asp Val Val Gly Tyr Pro Phe Met Pro Pro Tyr Trp Gly Leu Gly 
385                 390                 395                 400 


Phe His Leu Cys Arg Trp Gly Tyr Ser Ser Thr Ala Ile Thr Arg Gln 
                405                 410                 415     


Val Val Glu Asn Met Thr Arg Ala His Phe Pro Leu Asp Val Gln Trp 
            420                 425                 430         


Asn Asp Leu Asp Tyr Met Asp Ser Arg Arg Asp Phe Thr Phe Asn Lys 
        435                 440                 445             


Asp Gly Phe Arg Asp Phe Pro Ala Met Val Gln Glu Leu His Gln Gly 
    450                 455                 460                 


Gly Arg Arg Tyr Met Met Ile Val Asp Pro Ala Ile Ser Ser Ser Gly 
465                 470                 475                 480 


Pro Ala Gly Ser Tyr Arg Pro Tyr Asp Glu Gly Leu Arg Arg Gly Val 
                485                 490                 495     


Phe Ile Thr Asn Glu Thr Gly Gln Pro Leu Ile Gly Lys Val Trp Pro 
            500                 505                 510         


Gly Ser Thr Ala Phe Pro Asp Phe Thr Asn Pro Thr Ala Leu Ala Trp 
        515                 520                 525             


Trp Glu Asp Met Val Ala Glu Phe His Asp Gln Val Pro Phe Asp Gly 
    530                 535                 540                 


Met Trp Ile Asp Met Asn Glu Pro Ser Asn Phe Ile Arg Gly Ser Glu 
545                 550                 555                 560 


Asp Gly Cys Pro Asn Asn Glu Leu Glu Asn Pro Pro Tyr Val Pro Gly 
                565                 570                 575     


Val Val Gly Gly Thr Leu Gln Ala Ala Thr Ile Cys Ala Ser Ser His 
            580                 585                 590         


Gln Phe Leu Ser Thr His Tyr Asn Leu His Asn Leu Tyr Gly Leu Thr 
        595                 600                 605             


Glu Ala Ile Ala Ser His Arg Ala Leu Val Lys Ala Arg Gly Thr Arg 
    610                 615                 620                 


Pro Phe Val Ile Ser Arg Ser Thr Phe Ala Gly His Gly Arg Tyr Ala 
625                 630                 635                 640 


Gly His Trp Thr Gly Asp Val Trp Ser Ser Trp Glu Gln Leu Ala Ser 
                645                 650                 655     


Ser Val Pro Glu Ile Leu Gln Phe Asn Leu Leu Gly Val Pro Leu Val 
            660                 665                 670         


Gly Ala Asp Val Cys Gly Phe Leu Gly Asn Thr Ser Glu Glu Leu Cys 
        675                 680                 685             


Val Arg Trp Thr Gln Leu Gly Ala Phe Tyr Pro Phe Met Arg Asn His 
    690                 695                 700                 


Asn Ser Leu Leu Ser Leu Pro Gln Glu Pro Tyr Ser Phe Ser Glu Pro 
705                 710                 715                 720 


Ala Gln Gln Ala Met Arg Lys Ala Leu Thr Leu Arg Tyr Ala Leu Leu 
                725                 730                 735     


Pro His Leu Tyr Thr Leu Phe His Gln Ala His Val Ala Gly Glu Thr 
            740                 745                 750         


Val Ala Arg Pro Leu Phe Leu Glu Phe Pro Lys Asp Ser Ser Thr Trp 
        755                 760                 765             


Thr Val Asp His Gln Leu Leu Trp Gly Glu Ala Leu Leu Ile Thr Pro 
    770                 775                 780                 


Val Leu Gln Ala Gly Lys Ala Glu Val Thr Gly Tyr Phe Pro Leu Gly 
785                 790                 795                 800 


Thr Trp Tyr Asp Leu Gln Thr Val Pro Ile Glu Ala Leu Gly Ser Leu 
                805                 810                 815     


Pro Pro Pro Pro Ala Ala Pro Arg Glu Pro Ala Ile His Ser Glu Gly 
            820                 825                 830         


Gln Trp Val Thr Leu Pro Ala Pro Leu Asp Thr Ile Asn Val His Leu 
        835                 840                 845             


Arg Ala Gly Tyr Ile Ile Pro Leu Gln Gly Pro Gly Leu Thr Thr Thr 
    850                 855                 860                 


Glu Ser Arg Gln Gln Pro Met Ala Leu Ala Val Ala Leu Thr Lys Gly 
865                 870                 875                 880 


Gly Glu Ala Arg Gly Glu Leu Phe Trp Asp Asp Gly Glu Ser Leu Glu 
                885                 890                 895     


Val Leu Glu Arg Gly Ala Tyr Thr Gln Val Ile Phe Leu Ala Arg Asn 
            900                 905                 910         


Asn Thr Ile Val Asn Glu Leu Val Arg Val Thr Ser Glu Gly Ala Gly 
        915                 920                 925             


Leu Gln Leu Gln Lys Val Thr Val Leu Gly Val Ala Thr Ala Pro Gln 
    930                 935                 940                 


Gln Val Leu Ser Asn Gly Val Pro Val Ser Asn Phe Thr Tyr Ser Pro 
945                 950                 955                 960 


Asp Thr Lys Val Leu Asp Ile Cys Val Ser Leu Leu Met Gly Glu Gln 
                965                 970                 975     


Phe Leu Val Ser Trp Cys 
            980         


<210>  26
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miRNA target sequence

<400>  26
agtgaattct accagtgcca ta                                                22


<210>  27
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miRNA target sequence

<400>  27
agtgtgagtt ctaccattgc caaa                                              24


<210>  28
<211>  6
<212>  PRT
<213>  Homo sapiens

<400>  28

Trp Ile Asp Met Asn Glu 
1               5       


<210>  29
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  29

Thr Val Pro Ile Glu Ala Leu 
1               5           


<210>  30
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  30

Gln Thr Val Pro Ile Glu Ala Leu Gly 
1               5                   


<210>  31
<211>  25
<212>  PRT
<213>  Homo sapiens

<400>  31

Pro Leu Gly Thr Trp Tyr Asp Leu Gln Thr Val Pro Ile Glu Ala Leu 
1               5                   10                  15      


Gly Ser Leu Pro Pro Pro Pro Ala Ala 
            20                  25  


