                               SEQUENCE LISTING

<110> CHAN ZUCKERBERG BIOHUB, INC.
      THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR 
      UNIVERSITY
 
<120> A NOVEL CHROMATIN-OPENING ELEMENT FOR STABLE LONG TERM GENE 
      EXPRESSION

<130> 103182-1180463-002610WO

<140>
<141>

<150> 62/841,151
<151> 2019-04-30

<160> 40    

<170> PatentIn version 3.5

<210> 1
<211> 1002
<212> DNA
<213> Homo sapiens

<400> 1
gcacacgacc acaattccac tgaaagcatt ttaatacgga acttgtcact cccagggagc       60

ctccgctcag ccggcagttg gttcatttca atccccacga caacccttca aagtgcaggg      120

cagacagcag gtggctctgc ccaggcgcct ggatcacagc ccggcctgca gccctcacct      180

gggcgcgggg agaccctgag gacgctcctc caggcggcgc tggccggggc ctgcggacac      240

ggacgggcgg gctgagctcc gggacccctc cccgcgcccc gcaccccgca ccccgcaccc      300

cgcaccccgc acccggcgct cacccgtccc agccccgccg cccgcagccc cagctgcaac      360

gcagccaccg ccgccatcgc acccggcccc gcgggcgctt ccgggacgca ggaggcatct      420

gcatccgggg cgccgctgag tcccgcccag agccccgccc ccggctccag gttctgcgag      480

cggcttccgc cgggctgctc cgcgggcgcg tcggccatga gcgagttgcc gggcgacgtg      540

cgggcgtttc tgcgggagca cccgagcctg cggctccaga cggacgcccg caaggttcgc      600

agcgcgggag gggaacggag tggcggagaa gggcgcagtt gggatgaggg gctgagggga      660

gggcagggga gaggagaggg caggggagag gggagagggg agagcaggag agaggggaag      720

gcaggggaga gggcgcggcg ggatcagggg aggagaggga agggggcgcg gcaggagggg      780

gcaccaggga gcggagccct ggccctcctg acgtcctgcc cgcccacgcg tccgcaggtg      840

aggtgcatcc tgacaggtca cgagctgccc tgccgcctgc cggagctcca ggtctacacc      900

cgcggcaaaa agtaccagcg gctggtccgc gcctccccgg ccttcgacta tgcagagttc      960

gagccgcaca tcgtgcccag caccaagaac ccgtaggtgg tc                        1002


<210> 2
<211> 896
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 2
ttcaaagtgc agggcagaca gcaggtggct ctgcccaggc gcctggatca cagcccggcc       60

tgcagccctc acctgggcgc ggggagaccc tgaggacgct cctccaggcg gcgctggccg      120

gggcctgcgg acacggacgg gcgggctgag ctccgggacc cctccccgcg ccccgcaccc      180

cgcaccccgc accccgcacc ccgcacccgg cgctcacccg tcccagcccc gccgcccgca      240

gccccagctg caacgcagcc accgccgcca tcgcacccgg ccccgcgggc gcttccggga      300

cgcaggaggc atctgcatcc ggggcgccgc tgagtcccgc ccagagcccc gcccccggct      360

ccaggttctg cgagcggctt ccgccgggct gctccgcggg cgcgtcggcc atgagcgagt      420

tgccgggcga cgtgcgggcg tttctgcggg agcacccgag cctgcggctc cagacggacg      480

cccgcaaggt tcgcagcgcg ggaggggaac ggagtggcgg agaagggcgc agttgggatg      540

aggggctgag gggagggcag gggagaggag agggcagggg agaggggaga ggggagagca      600

ggagagaggg gaaggcaggg gagagggcgc ggcgggatca ggggaggaga gggaaggggg      660

cgcggcagga gggggcacca gggagcggag ccctggccct cctgacgtcc tgcccgccca      720

cgcgtccgca ggtgaggtgc atcctgacag gtcacgagct gccctgccgc ctgccggagc      780

tccaggtcta cacccgcggc aaaaagtacc agcggctggt ccgcgcctcc ccggccttcg      840

actatgcaga gttcgagccg cacatcgtgc ccagcaccaa gaacccgtag gtggtc          896


<210> 3
<211> 524
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 3
agcggcttcc gccgggctgc tccgcgggcg cgtcggccat gagcgagttg ccgggcgacg       60

tgcgggcgtt tctgcgggag cacccgagcc tgcggctcca gacggacgcc cgcaaggttc      120

gcagcgcggg aggggaacgg agtggcggag aagggcgcag ttgggatgag gggctgaggg      180

gagggcaggg gagaggagag ggcaggggag aggggagagg ggagagcagg agagagggga      240

aggcagggga gagggcgcgg cgggatcagg ggaggagagg gaagggggcg cggcaggagg      300

gggcaccagg gagcggagcc ctggccctcc tgacgtcctg cccgcccacg cgtccgcagg      360

tgaggtgcat cctgacaggt cacgagctgc cctgccgcct gccggagctc caggtctaca      420

cccgcggcaa aaagtaccag cggctggtcc gcgcctcccc ggccttcgac tatgcagagt      480

tcgagccgca catcgtgccc agcaccaaga acccgtaggt ggtc                       524


<210> 4
<211> 761
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 4
gcacacgacc acaattccac tgaaagcatt ttaatacgga acttgtcact cccagggagc       60

ctccgctcag ccggcagttg gttcatttca atccccacga caacccttca aagtgcaggg      120

cagacagcag gtggctctgc ccaggcgcct ggatcacagc ccggcctgca gccctcacct      180

gggcgcgggg agaccctgag gacgctcctc caggcggcgc tggccggggc ctgcggacac      240

ggacgggcgg gctgagctcc gggacccctc cccgcgcccc gcaccccgca ccccgcaccc      300

cgcaccccgc acccggcgct cacccgtccc agccccgccg cccgcagccc cagctgcaac      360

gcagccaccg ccgccatcgc acccggcccc gcgggcgctt ccgggacgca ggaggcatct      420

gcatccgggg cgccgctgag tcccgcccag agccccgccc ccggctccag gttctgcgag      480

cggcttccgc cgggctgctc cgcgggcgcg tcggccatga gcgagttgcc gggcgacgtg      540

cgggcgtttc tgcgggagca cccgagcctg cggctccaga cggacgcccg caaggttcgc      600

agcgcgggag gggaacggag tggcggagaa gggcgcagtt gggatgaggg gctgagggga      660

gggcagggga gaggagaggg caggggagag gggagagggg agagcaggag agaggggaag      720

gcaggggaga gggcgcggcg ggatcagggg aggagaggga a                          761


<210> 5
<211> 283
<212> DNA
<213> Homo sapiens

<400> 5
agcggcttcc gccgggctgc tccgcgggcg cgtcggccat gagcgagttg ccgggcgacg       60

tgcgggcgtt tctgcgggag cacccgagcc tgcggctcca gacggacgcc cgcaaggttc      120

gcagcgcggg aggggaacgg agtggcggag aagggcgcag ttgggatgag gggctgaggg      180

gagggcaggg gagaggagag ggcaggggag aggggagagg ggagagcagg agagagggga      240

aggcagggga gagggcgcgg cgggatcagg ggaggagagg gaa                        283


<210> 6
<211> 262
<212> DNA
<213> Rous sarcoma virus

<400> 6
aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca       60

tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga      120

tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt      180

ccgcattgca gagatattgt atttaagtgc ctagctcgat acaataaacg ccatttgacc      240

attcaccaca ttggtgtgca cc                                               262


<210> 7
<211> 589
<212> DNA
<213> Cytomegalovirus sp. 

<400> 7
gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata       60

gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc      120

ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag      180

ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac      240

atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg      300

cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg      360

tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat      420

agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt      480

tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc      540

aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctct                  589


<210> 8
<211> 543
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      Ef1-alpha promoter sequence

<400> 8
gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga       60

gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa      120

ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta      180

tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca      240

gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg      300

ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc      360

gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc      420

ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcacct      480

ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc      540

tac                                                                    543


<210> 9
<211> 515
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      PGK promoter sequence

<400> 9
ccacggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct       60

gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca      120

cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc      180

ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac      240

aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg      300

cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcgggc gcgccgagag      360

cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt      420

cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc      480

cctcgttgac cgaatcaccg acctctctcc ccagg                                 515


<210> 10
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 10
actaagagtc gacccatctt gacggcagcg ata                                    33


<210> 11
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 11
tagttctgct agccgctgag acgatctcgg aaa                                    33


<210> 12
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 12
actaagagtc gaccgctgag acgatctcgg aaa                                    33


<210> 13
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 13
tagttctgct agcccatctt gacggcagcg ata                                    33


<210> 14
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 14
actaagagtc gactcaccct cacggttagc tact                                   34


<210> 15
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 15
tagttctgct agccaacgta caacgcagca ctc                                    33


<210> 16
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 16
actaagagtc gaccagaccg atctgattca ctgg                                   34


<210> 17
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 17
actaagagtc gacacttttc cacacactac ttccctc                                37


<210> 18
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 18
tagttctgct agctctgtct ttccagcagc gtt                                    33


<210> 19
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 19
actaagagtc gacgcacacg accacaattc cac                                    33


<210> 20
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 20
tagttctgct agcgaccacc tacgggttct tgg                                    33


<210> 21
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 21
actaagagtc gacgaccacc tacgggttct tgg                                    33


<210> 22
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 22
tagttctgct agcgcacacg accacaattc cac                                    33


<210> 23
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 23
actaagagtc gacaagcaca cggccctaga aat                                    33


<210> 24
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 24
tagttctgct agctggagag gaaaactacc ggc                                    33


<210> 25
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 25
actaagagtc gacgtcctgc ccacgtatct acc                                    33


<210> 26
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 26
tagttctgct agcctagcga ggagttagca cgg                                    33


<210> 27
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 27
actaagagtc gacctagcga ggagttagca cgg                                    33


<210> 28
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 28
tagttctgct agcgtcctgc ccacgtatct acc                                    33


<210> 29
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 29
actaagagtc gaccaagttc actgtgtgct gtgtatt                                37


<210> 30
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 30
tagttctgct agcgtcttcg ttgccaacag gct                                    33


<210> 31
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 31
actaagagtc gacgaggggt tgggggtaaa attagt                                 36


<210> 32
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 32
tagttctgct agcaggttcc ttagtgggca aca                                    33


<210> 33
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 33
actaagagtc gacagcaggg aaagcgagag aac                                    33


<210> 34
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 34
tagttctgct agcaaaggcc ttcccactga tcg                                    33


<210> 35
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 35
actaagagtc gacaaaggcc ttcccactga tcg                                    33


<210> 36
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 36
tagttctgct agcagcaggg aaagcgagag aac                                    33


<210> 37
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 37
actaagagtc gacttcaaag tgcagggcag aca                                    33


<210> 38
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 38
actaagagtc gacttctgcg agcggcttcc                                        30


<210> 39
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 39
tagttctgct agcttccctc tcctcccctg atc                                    33


<210> 40
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 40
tagttctgct agccggtcgc ataggccgag                                        30


