               SEQUENCE LISTING

<110> Medizinische Hochschule Hannover

<120> Artificial signalling molecule

<130> M1084PCT

<150> EP19172048.1
<151> 2019-04-30

<160> 21

<170> BiSSAP 1.3.6

<210> 1
<211> 298
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..24
<223> signal peptide

<220> 
<223> antigen domain HLA-A*02:01

<220> 
<221> CHAIN
<222> 25..114
<223> alpha 1 domain

<220> 
<221> CHAIN
<222> 115..206
<223> alpha 2 domain

<220> 
<221> CHAIN
<222> 207..298
<223> alpha 3 domain

<400> 1
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala 
1               5                   10                  15      
Leu Ala Leu Thr Gln Thr Trp Ala Gly Ser His Ser Met Arg Tyr Phe 
            20                  25                  30          
Phe Thr Ser Val Ser Arg Pro Gly Arg Gly Glu Pro Arg Phe Ile Ala 
        35                  40                  45              
Val Gly Tyr Val Asp Asp Thr Gln Phe Val Arg Phe Asp Ser Asp Ala 
    50                  55                  60                  
Ala Ser Gln Arg Met Glu Pro Arg Ala Pro Trp Ile Glu Gln Glu Gly 
65                  70                  75                  80  
Pro Glu Tyr Trp Asp Gly Glu Thr Arg Lys Val Lys Ala His Ser Gln 
                85                  90                  95      
Thr His Arg Val Asp Leu Gly Thr Leu Arg Gly Tyr Tyr Asn Gln Ser 
            100                 105                 110         
Glu Ala Gly Ser His Thr Val Gln Arg Met Tyr Gly Cys Asp Val Gly 
        115                 120                 125             
Ser Asp Trp Arg Phe Leu Arg Gly Tyr His Gln Tyr Ala Tyr Asp Gly 
    130                 135                 140                 
Lys Asp Tyr Ile Ala Leu Lys Glu Asp Leu Arg Ser Trp Thr Ala Ala 
145                 150                 155                 160 
Asp Met Ala Ala Gln Thr Thr Lys His Lys Trp Glu Ala Ala His Val 
                165                 170                 175     
Ala Glu Gln Leu Arg Ala Tyr Leu Glu Gly Thr Cys Val Glu Trp Leu 
            180                 185                 190         
Arg Arg Tyr Leu Glu Asn Gly Lys Glu Thr Leu Gln Arg Thr Asp Ala 
        195                 200                 205             
Pro Lys Thr His Met Thr His His Ala Val Ser Asp His Glu Ala Thr 
    210                 215                 220                 
Leu Arg Cys Trp Ala Leu Ser Phe Tyr Pro Ala Glu Ile Thr Leu Thr 
225                 230                 235                 240 
Trp Gln Arg Asp Gly Glu Asp Gln Thr Gln Asp Thr Glu Leu Val Glu 
                245                 250                 255     
Thr Arg Pro Ala Gly Asp Gly Thr Phe Gln Lys Trp Ala Ala Val Val 
            260                 265                 270         
Val Pro Ser Gly Gln Glu Gln Arg Tyr Thr Cys His Val Gln His Glu 
        275                 280                 285             
Gly Leu Pro Lys Pro Leu Thr Leu Arg Trp 
    290                 295             

<210> 2
<211> 257
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..25
<223> signal peptide

<220> 
<223> fusion protein

<220> 
<221> CHAIN
<222> 26..109
<223> alpha 1 domain

<220> 
<221> CHAIN
<222> 110..203
<223> alpha 2 domain

<220> 
<221> CHAIN
<222> 204..216
<223> extracellular portion of transmembrane domain

<220> 
<221> CHAIN
<222> 217..223
<223> linker

<220> 
<221> CHAIN
<222> 224..257
<223> Jun zipper

<400> 2
Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      
Leu Met Ser Ala Gln Glu Ser Trp Ala Ile Lys Glu Glu His Val Ile 
            20                  25                  30          
Ile Gln Ala Glu Phe Tyr Leu Asn Pro Asp Gln Ser Gly Glu Phe Met 
        35                  40                  45              
Phe Asp Phe Asp Gly Asp Glu Ile Phe His Val Asp Met Ala Lys Lys 
    50                  55                  60                  
Glu Thr Val Trp Arg Leu Glu Glu Phe Gly Arg Phe Ala Ser Phe Glu 
65                  70                  75                  80  
Ala Gln Gly Ala Leu Ala Asn Ile Ala Val Asp Lys Ala Asn Leu Glu 
                85                  90                  95      
Ile Met Thr Lys Arg Ser Asn Tyr Thr Pro Ile Thr Asn Val Pro Pro 
            100                 105                 110         
Glu Val Thr Val Leu Thr Asn Ser Pro Val Glu Leu Arg Glu Pro Asn 
        115                 120                 125             
Val Leu Ile Cys Phe Ile Asp Lys Phe Thr Pro Pro Val Val Asn Val 
    130                 135                 140                 
Thr Trp Leu Arg Asn Gly Lys Pro Val Thr Thr Gly Val Ser Glu Thr 
145                 150                 155                 160 
Val Phe Leu Pro Arg Glu Asp His Leu Phe Arg Lys Phe His Tyr Leu 
                165                 170                 175     
Pro Phe Leu Pro Ser Thr Glu Asp Val Tyr Asp Cys Arg Val Glu His 
            180                 185                 190         
Trp Gly Leu Asp Glu Pro Leu Leu Lys His Trp Glu Phe Asp Ala Pro 
        195                 200                 205             
Ser Pro Leu Pro Glu Thr Thr Glu Ser Arg Gly Gly Gly Gly Gly Ala 
    210                 215                 220                 
Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Glu Leu 
225                 230                 235                 240 
Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala Gln Leu Lys Gln 
                245                 250                 255     
Ser 
    

<210> 3
<211> 267
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..29
<223> signal peptide

<220> 
<223> fusion protein

<220> 
<221> CHAIN
<222> 30..123
<223> beta 1 chain

<220> 
<221> CHAIN
<222> 124..217
<223> beta 2 chain

<220> 
<221> CHAIN
<222> 218..227
<223> extracellular part of transmembrane domain

<220> 
<221> CHAIN
<222> 228..234
<223> linker

<220> 
<221> CHAIN
<222> 235..267
<223> Fos zipper

<400> 3
Met Val Cys Leu Lys Leu Pro Gly Gly Ser Cys Met Thr Ala Leu Thr 
1               5                   10                  15      
Val Thr Leu Met Val Leu Ser Ser Pro Leu Ala Leu Ala Gly Asp Thr 
            20                  25                  30          
Arg Pro Arg Phe Leu Trp Gln Leu Lys Phe Glu Cys His Phe Phe Asn 
        35                  40                  45              
Gly Thr Glu Arg Val Arg Leu Leu Glu Arg Cys Ile Tyr Asn Gln Glu 
    50                  55                  60                  
Glu Ser Val Arg Phe Asp Ser Asp Val Gly Glu Tyr Arg Ala Val Thr 
65                  70                  75                  80  
Glu Leu Gly Arg Pro Asp Ala Glu Tyr Trp Asn Ser Gln Lys Asp Leu 
                85                  90                  95      
Leu Glu Gln Arg Arg Ala Ala Val Asp Thr Tyr Cys Arg His Asn Tyr 
            100                 105                 110         
Gly Val Gly Glu Ser Phe Thr Val Gln Arg Arg Val Glu Pro Lys Val 
        115                 120                 125             
Thr Val Tyr Pro Ser Lys Thr Gln Pro Leu Gln His His Asn Leu Leu 
    130                 135                 140                 
Val Cys Ser Val Ser Gly Phe Tyr Pro Gly Ser Ile Glu Val Arg Trp 
145                 150                 155                 160 
Phe Arg Asn Gly Gln Glu Glu Lys Ala Gly Val Val Ser Thr Gly Leu 
                165                 170                 175     
Ile Gln Asn Gly Asp Trp Thr Phe Gln Thr Leu Val Met Leu Glu Thr 
            180                 185                 190         
Val Pro Arg Ser Gly Glu Val Tyr Thr Cys Gln Val Glu His Pro Ser 
        195                 200                 205             
Val Thr Ser Pro Leu Thr Val Glu Trp Arg Ala Arg Ser Glu Ser Ala 
    210                 215                 220                 
Gln Ser Lys Ser Arg Gly Gly Gly Gly Gly Asp Thr Leu Gln Ala Glu 
225                 230                 235                 240 
Thr Asp Gln Leu Glu Asp Glu Lys Ser Ala Leu Gln Thr Glu Ile Ala 
                245                 250                 255     
Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe 
            260                 265         

<210> 4
<211> 34
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..4
<223> linker

<220> 
<223> linker domain-acidic zipper domain

<220> 
<221> CHAIN
<222> 5..34
<223> acidic zipper

<400> 4
Ser Arg Gly Thr Ala Gln Leu Glu Lys Glu Leu Gln Ala Leu Glu Lys 
1               5                   10                  15      
Glu Asn Ala Gln Leu Glu Trp Glu Leu Gln Ala Leu Glu Lys Glu Leu 
            20                  25                  30          
Ala Gln 
        

<210> 5
<211> 34
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..4
<223> linker

<220> 
<223> linker domain-basic zipper domain

<220> 
<221> CHAIN
<222> 5..34
<223> basic zipper

<400> 5
Ser Arg Gly Thr Ala Gln Leu Lys Lys Lys Leu Gln Ala Leu Lys Lys 
1               5                   10                  15      
Lys Asn Ala Gln Leu Lys Trp Lys Leu Gln Ala Leu Lys Lys Lys Leu 
            20                  25                  30          
Ala Gln 
        

<210> 6
<211> 47
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..4
<223> linker

<220> 
<223> linker domain-REL zipper domain

<220> 
<221> CHAIN
<222> 5..47
<223> REL zipper

<400> 6
Ser Arg Gly Ser Leu Glu Ile Arg Ala Ala Phe Leu Arg Gln Arg Asn 
1               5                   10                  15      
Thr Ala Leu Arg Thr Glu Val Ala Glu Leu Glu Gln Glu Val Gln Arg 
            20                  25                  30          
Leu Glu Asn Glu Val Ser Gln Tyr Glu Thr Arg Tyr Gly Pro Leu 
        35                  40                  45          

<210> 7
<211> 47
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..4
<223> linker

<220> 
<223> linker domain-ERL zipper domain

<220> 
<221> CHAIN
<222> 5..47
<223> ERL zipper

<400> 7
Ser Arg Gly Thr Leu Glu Ile Glu Ala Ala Phe Leu Glu Arg Glu Asn 
1               5                   10                  15      
Thr Ala Leu Glu Thr Arg Val Ala Glu Leu Arg Gln Arg Val Gln Arg 
            20                  25                  30          
Leu Arg Asn Arg Val Ser Gln Tyr Arg Thr Arg Tyr Gly Pro Leu 
        35                  40                  45          

<210> 8
<211> 873
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..24
<223> signal peptide

<220> 
<223> CLR with short spacer

<220> 
<221> CHAIN
<222> 25..114
<223> alpha 1 domain

<220> 
<221> CHAIN
<222> 115..206
<223> alpha 2 domain

<220> 
<221> CHAIN
<222> 207..298
<223> alpha 3 domain

<220> 
<221> CHAIN
<222> 299..310
<223> short spacer domain

<220> 
<221> CHAIN
<222> 311..338
<223> CD28 transmembrane domain

<220> 
<221> CHAIN
<222> 339..380
<223> 4-1BB costimulatory domain

<220> 
<221> CHAIN
<222> 381..492
<223> CD3 zeta signalling domain

<220> 
<221> CHAIN
<222> 493..516
<223> T2A element

<220> 
<221> CHAIN
<222> 517..538
<223> GM-CSF signal peptide

<220> 
<221> CHAIN
<222> 539..873
<223> truncated EGFR selection domain

<400> 8
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala 
1               5                   10                  15      
Leu Ala Leu Thr Gln Thr Trp Ala Gly Ser His Ser Met Arg Tyr Phe 
            20                  25                  30          
Phe Thr Ser Val Ser Arg Pro Gly Arg Gly Glu Pro Arg Phe Ile Ala 
        35                  40                  45              
Val Gly Tyr Val Asp Asp Thr Gln Phe Val Arg Phe Asp Ser Asp Ala 
    50                  55                  60                  
Ala Ser Gln Arg Met Glu Pro Arg Ala Pro Trp Ile Glu Gln Glu Gly 
65                  70                  75                  80  
Pro Glu Tyr Trp Asp Gly Glu Thr Arg Lys Val Lys Ala His Ser Gln 
                85                  90                  95      
Thr His Arg Val Asp Leu Gly Thr Leu Arg Gly Tyr Tyr Asn Gln Ser 
            100                 105                 110         
Glu Ala Gly Ser His Thr Val Gln Arg Met Tyr Gly Cys Asp Val Gly 
        115                 120                 125             
Ser Asp Trp Arg Phe Leu Arg Gly Tyr His Gln Tyr Ala Tyr Asp Gly 
    130                 135                 140                 
Lys Asp Tyr Ile Ala Leu Lys Glu Asp Leu Arg Ser Trp Thr Ala Ala 
145                 150                 155                 160 
Asp Met Ala Ala Gln Thr Thr Lys His Lys Trp Glu Ala Ala His Val 
                165                 170                 175     
Ala Glu Gln Leu Arg Ala Tyr Leu Glu Gly Thr Cys Val Glu Trp Leu 
            180                 185                 190         
Arg Arg Tyr Leu Glu Asn Gly Lys Glu Thr Leu Gln Arg Thr Asp Ala 
        195                 200                 205             
Pro Lys Thr His Met Thr His His Ala Val Ser Asp His Glu Ala Thr 
    210                 215                 220                 
Leu Arg Cys Trp Ala Leu Ser Phe Tyr Pro Ala Glu Ile Thr Leu Thr 
225                 230                 235                 240 
Trp Gln Arg Asp Gly Glu Asp Gln Thr Gln Asp Thr Glu Leu Val Glu 
                245                 250                 255     
Thr Arg Pro Ala Gly Asp Gly Thr Phe Gln Lys Trp Ala Ala Val Val 
            260                 265                 270         
Val Pro Ser Gly Gln Glu Gln Arg Tyr Thr Cys His Val Gln His Glu 
        275                 280                 285             
Gly Leu Pro Lys Pro Leu Thr Leu Arg Trp Glu Ser Lys Tyr Gly Pro 
    290                 295                 300                 
Pro Cys Pro Pro Cys Pro Met Phe Trp Val Leu Val Val Val Gly Gly 
305                 310                 315                 320 
Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
                325                 330                 335     
Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro 
            340                 345                 350         
Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys 
        355                 360                 365             
Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe 
    370                 375                 380                 
Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
385                 390                 395                 400 
Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
                405                 410                 415     
Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
            420                 425                 430         
Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
        435                 440                 445             
Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
    450                 455                 460                 
Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
465                 470                 475                 480 
Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Leu Glu Gly Gly 
                485                 490                 495     
Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn 
            500                 505                 510         
Pro Gly Pro Arg Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu 
        515                 520                 525             
Leu Pro His Pro Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly 
    530                 535                 540                 
Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn 
545                 550                 555                 560 
Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile 
                565                 570                 575     
Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu 
            580                 585                 590         
Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly 
        595                 600                 605             
Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala 
    610                 615                 620                 
Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln 
625                 630                 635                 640 
Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg 
                645                 650                 655     
Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys 
            660                 665                 670         
Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr 
        675                 680                 685             
Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys 
    690                 695                 700                 
Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys 
705                 710                 715                 720 
Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg 
                725                 730                 735     
Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg 
            740                 745                 750         
Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu 
        755                 760                 765             
Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys 
    770                 775                 780                 
Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys 
785                 790                 795                 800 
Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala 
                805                 810                 815     
Asp Ala Gly His Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly 
            820                 825                 830         
Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile 
        835                 840                 845             
Pro Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val 
    850                 855                 860                 
Val Ala Leu Gly Ile Gly Leu Phe Met 
865                 870             

<210> 9
<211> 1089
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..24
<223> signal peptide

<220> 
<223> CLR long spacer construct

<220> 
<221> CHAIN
<222> 25..114
<223> alpha 1 domain

<220> 
<221> CHAIN
<222> 115..206
<223> alpha 2 domain

<220> 
<221> CHAIN
<222> 207..298
<223> alpha 3 domain

<220> 
<221> CHAIN
<222> 299..526
<223> long spacer domain

<220> 
<221> CHAIN
<222> 527..554
<223> CD28 transmembrane domain

<220> 
<221> CHAIN
<222> 555..596
<223> 4-1BB costimulatory domain

<220> 
<221> CHAIN
<222> 597..708
<223> CD3 zeta signalling domain

<220> 
<221> CHAIN
<222> 709..732
<223> T2A element

<220> 
<221> CHAIN
<222> 734..754
<223> GM-CSF signal peptide

<220> 
<221> CHAIN
<222> 755..1089
<223> truncated EGFR selection domain

<400> 9
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala 
1               5                   10                  15      
Leu Ala Leu Thr Gln Thr Trp Ala Gly Ser His Ser Met Arg Tyr Phe 
            20                  25                  30          
Phe Thr Ser Val Ser Arg Pro Gly Arg Gly Glu Pro Arg Phe Ile Ala 
        35                  40                  45              
Val Gly Tyr Val Asp Asp Thr Gln Phe Val Arg Phe Asp Ser Asp Ala 
    50                  55                  60                  
Ala Ser Gln Arg Met Glu Pro Arg Ala Pro Trp Ile Glu Gln Glu Gly 
65                  70                  75                  80  
Pro Glu Tyr Trp Asp Gly Glu Thr Arg Lys Val Lys Ala His Ser Gln 
                85                  90                  95      
Thr His Arg Val Asp Leu Gly Thr Leu Arg Gly Tyr Tyr Asn Gln Ser 
            100                 105                 110         
Glu Ala Gly Ser His Thr Val Gln Arg Met Tyr Gly Cys Asp Val Gly 
        115                 120                 125             
Ser Asp Trp Arg Phe Leu Arg Gly Tyr His Gln Tyr Ala Tyr Asp Gly 
    130                 135                 140                 
Lys Asp Tyr Ile Ala Leu Lys Glu Asp Leu Arg Ser Trp Thr Ala Ala 
145                 150                 155                 160 
Asp Met Ala Ala Gln Thr Thr Lys His Lys Trp Glu Ala Ala His Val 
                165                 170                 175     
Ala Glu Gln Leu Arg Ala Tyr Leu Glu Gly Thr Cys Val Glu Trp Leu 
            180                 185                 190         
Arg Arg Tyr Leu Glu Asn Gly Lys Glu Thr Leu Gln Arg Thr Asp Ala 
        195                 200                 205             
Pro Lys Thr His Met Thr His His Ala Val Ser Asp His Glu Ala Thr 
    210                 215                 220                 
Leu Arg Cys Trp Ala Leu Ser Phe Tyr Pro Ala Glu Ile Thr Leu Thr 
225                 230                 235                 240 
Trp Gln Arg Asp Gly Glu Asp Gln Thr Gln Asp Thr Glu Leu Val Glu 
                245                 250                 255     
Thr Arg Pro Ala Gly Asp Gly Thr Phe Gln Lys Trp Ala Ala Val Val 
            260                 265                 270         
Val Pro Ser Gly Gln Glu Gln Arg Tyr Thr Cys His Val Gln His Glu 
        275                 280                 285             
Gly Leu Pro Lys Pro Leu Thr Leu Arg Trp Glu Ser Lys Tyr Gly Pro 
    290                 295                 300                 
Pro Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe 
305                 310                 315                 320 
Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
                325                 330                 335     
Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val 
            340                 345                 350         
Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
        355                 360                 365             
Lys Pro Arg Glu Glu Gln Phe Gln Ser Thr Tyr Arg Val Val Ser Val 
    370                 375                 380                 
Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
385                 390                 395                 400 
Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser 
                405                 410                 415     
Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
            420                 425                 430         
Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
        435                 440                 445             
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
    450                 455                 460                 
Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
465                 470                 475                 480 
Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp 
                485                 490                 495     
Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
            500                 505                 510         
Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys Met Phe 
        515                 520                 525             
Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu 
    530                 535                 540                 
Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys 
545                 550                 555                 560 
Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr 
                565                 570                 575     
Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly 
            580                 585                 590         
Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
        595                 600                 605             
Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
    610                 615                 620                 
Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
625                 630                 635                 640 
Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
                645                 650                 655     
Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
            660                 665                 670         
Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
        675                 680                 685             
Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
    690                 695                 700                 
Leu Pro Pro Arg Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu 
705                 710                 715                 720 
Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Arg Met Leu Leu Leu 
                725                 730                 735     
Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala Phe Leu Leu 
            740                 745                 750         
Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp 
        755                 760                 765             
Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr 
    770                 775                 780                 
Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp 
785                 790                 795                 800 
Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu 
                805                 810                 815     
Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro 
            820                 825                 830         
Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg 
        835                 840                 845             
Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu 
    850                 855                 860                 
Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly 
865                 870                 875                 880 
Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile 
                885                 890                 895     
Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile 
            900                 905                 910         
Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His 
        915                 920                 925             
Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys 
    930                 935                 940                 
Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys 
945                 950                 955                 960 
Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys 
                965                 970                 975     
Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys 
            980                 985                 990         
Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp 
        995                 1000                1005            
Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn 
    1010                1015                1020                
Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His Leu 
1025                1030                1035                1040
Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly 
                1045                1050                1055    
Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val 
            1060                1065                1070        
Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe 
        1075                1080                1085            
Met 
    

<210> 10
<211> 832
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..25
<223> signal peptide

<220> 
<223> HLA-DRA*01:01 CLR construct with Jun-zipper

<220> 
<221> CHAIN
<222> 26..109
<223> alpha 1 domain

<220> 
<221> CHAIN
<222> 110..203
<223> alpha 2 domain

<220> 
<221> CHAIN
<222> 204..216
<223> extracellular part of transmembrane domain

<220> 
<221> CHAIN
<222> 217..223
<223> linker

<220> 
<221> CHAIN
<222> 224..257
<223> Jun-Zipper

<220> 
<221> CHAIN
<222> 258..269
<223> short spacer domain

<220> 
<221> CHAIN
<222> 270..297
<223> CD28 transmembrane domain

<220> 
<221> CHAIN
<222> 298..339
<223> 4-1BB costimulatory domain

<220> 
<221> CHAIN
<222> 340..451
<223> CD32 zeta signalling domain

<220> 
<221> CHAIN
<222> 452..475
<223> T2A element

<220> 
<221> CHAIN
<222> 476..497
<223> GM-CSF signal peptide

<220> 
<221> CHAIN
<222> 498..832
<223> truncated EGFR selection domain

<400> 10
Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      
Leu Met Ser Ala Gln Glu Ser Trp Ala Ile Lys Glu Glu His Val Ile 
            20                  25                  30          
Ile Gln Ala Glu Phe Tyr Leu Asn Pro Asp Gln Ser Gly Glu Phe Met 
        35                  40                  45              
Phe Asp Phe Asp Gly Asp Glu Ile Phe His Val Asp Met Ala Lys Lys 
    50                  55                  60                  
Glu Thr Val Trp Arg Leu Glu Glu Phe Gly Arg Phe Ala Ser Phe Glu 
65                  70                  75                  80  
Ala Gln Gly Ala Leu Ala Asn Ile Ala Val Asp Lys Ala Asn Leu Glu 
                85                  90                  95      
Ile Met Thr Lys Arg Ser Asn Tyr Thr Pro Ile Thr Asn Val Pro Pro 
            100                 105                 110         
Glu Val Thr Val Leu Thr Asn Ser Pro Val Glu Leu Arg Glu Pro Asn 
        115                 120                 125             
Val Leu Ile Cys Phe Ile Asp Lys Phe Thr Pro Pro Val Val Asn Val 
    130                 135                 140                 
Thr Trp Leu Arg Asn Gly Lys Pro Val Thr Thr Gly Val Ser Glu Thr 
145                 150                 155                 160 
Val Phe Leu Pro Arg Glu Asp His Leu Phe Arg Lys Phe His Tyr Leu 
                165                 170                 175     
Pro Phe Leu Pro Ser Thr Glu Asp Val Tyr Asp Cys Arg Val Glu His 
            180                 185                 190         
Trp Gly Leu Asp Glu Pro Leu Leu Lys His Trp Glu Phe Asp Ala Pro 
        195                 200                 205             
Ser Pro Leu Pro Glu Thr Thr Glu Ser Arg Gly Gly Gly Gly Gly Ala 
    210                 215                 220                 
Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Glu Leu 
225                 230                 235                 240 
Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala Gln Leu Lys Gln 
                245                 250                 255     
Ser Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Met Phe Trp 
            260                 265                 270         
Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
        275                 280                 285             
Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys Leu 
    290                 295                 300                 
Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln 
305                 310                 315                 320 
Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly 
                325                 330                 335     
Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
            340                 345                 350         
Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
        355                 360                 365             
Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
    370                 375                 380                 
Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
385                 390                 395                 400 
Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
                405                 410                 415     
Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
            420                 425                 430         
Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
        435                 440                 445             
Pro Pro Arg Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr 
    450                 455                 460                 
Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Arg Met Leu Leu Leu Val 
465                 470                 475                 480 
Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala Phe Leu Leu Ile 
                485                 490                 495     
Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser 
            500                 505                 510         
Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser 
        515                 520                 525             
Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser 
    530                 535                 540                 
Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys 
545                 550                 555                 560 
Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu 
                565                 570                 575     
Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly 
            580                 585                 590         
Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn 
        595                 600                 605             
Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp 
    610                 615                 620                 
Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn 
625                 630                 635                 640 
Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser 
                645                 650                 655     
Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala 
            660                 665                 670         
Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val 
        675                 680                 685             
Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn 
    690                 695                 700                 
Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile 
705                 710                 715                 720 
Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr 
                725                 730                 735     
Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly 
            740                 745                 750         
Pro His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn 
        755                 760                 765             
Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys 
    770                 775                 780                 
His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys 
785                 790                 795                 800 
Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly 
                805                 810                 815     
Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met 
            820                 825                 830         



<210> 11
<211> 842
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..29
<223> signal peptide

<220> 
<223> HLA-DRB1*01:01 CLR short construct with Fos-zipper

<220> 
<221> CHAIN
<222> 30..123
<223> beta 1 domain

<220> 
<221> CHAIN
<222> 124..217
<223> beta 2 domain

<220> 
<221> CHAIN
<222> 218..227
<223> extracellular part of transmembrane domain

<220> 
<221> CHAIN
<222> 228..234
<223> linker

<220> 
<221> CHAIN
<222> 235..267
<223> Fos-Zipper

<220> 
<221> CHAIN
<222> 268..279
<223> short hinge domain short spacer domain

<220> 
<221> CHAIN
<222> 280..307
<223> CD28 transmembrane domain

<220> 
<221> CHAIN
<222> 308..349
<223> 4-1BB costimulatory domain

<220> 
<221> CHAIN
<222> 350..461
<223> CD3 zeta signalling domain

<220> 
<221> CHAIN
<222> 462..485
<223> T2A element

<220> 
<221> CHAIN
<222> 486..507
<223> GM-CSF signal peptide

<220> 
<221> CHAIN
<222> 508..842
<223> truncated EGFR selection domain

<400> 11
Met Val Cys Leu Lys Leu Pro Gly Gly Ser Cys Met Thr Ala Leu Thr 
1               5                   10                  15      
Val Thr Leu Met Val Leu Ser Ser Pro Leu Ala Leu Ala Gly Asp Thr 
            20                  25                  30          
Arg Pro Arg Phe Leu Trp Gln Leu Lys Phe Glu Cys His Phe Phe Asn 
        35                  40                  45              
Gly Thr Glu Arg Val Arg Leu Leu Glu Arg Cys Ile Tyr Asn Gln Glu 
    50                  55                  60                  
Glu Ser Val Arg Phe Asp Ser Asp Val Gly Glu Tyr Arg Ala Val Thr 
65                  70                  75                  80  
Glu Leu Gly Arg Pro Asp Ala Glu Tyr Trp Asn Ser Gln Lys Asp Leu 
                85                  90                  95      
Leu Glu Gln Arg Arg Ala Ala Val Asp Thr Tyr Cys Arg His Asn Tyr 
            100                 105                 110         
Gly Val Gly Glu Ser Phe Thr Val Gln Arg Arg Val Glu Pro Lys Val 
        115                 120                 125             
Thr Val Tyr Pro Ser Lys Thr Gln Pro Leu Gln His His Asn Leu Leu 
    130                 135                 140                 
Val Cys Ser Val Ser Gly Phe Tyr Pro Gly Ser Ile Glu Val Arg Trp 
145                 150                 155                 160 
Phe Arg Asn Gly Gln Glu Glu Lys Ala Gly Val Val Ser Thr Gly Leu 
                165                 170                 175     
Ile Gln Asn Gly Asp Trp Thr Phe Gln Thr Leu Val Met Leu Glu Thr 
            180                 185                 190         
Val Pro Arg Ser Gly Glu Val Tyr Thr Cys Gln Val Glu His Pro Ser 
        195                 200                 205             
Val Thr Ser Pro Leu Thr Val Glu Trp Arg Ala Arg Ser Glu Ser Ala 
    210                 215                 220                 
Gln Ser Lys Ser Arg Gly Gly Gly Gly Gly Asp Thr Leu Gln Ala Glu 
225                 230                 235                 240 
Thr Asp Gln Leu Glu Asp Glu Lys Ser Ala Leu Gln Thr Glu Ile Ala 
                245                 250                 255     
Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe Glu Ser Lys Tyr Gly 
            260                 265                 270         
Pro Pro Cys Pro Pro Cys Pro Met Phe Trp Val Leu Val Val Val Gly 
        275                 280                 285             
Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
    290                 295                 300                 
Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
305                 310                 315                 320 
Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
                325                 330                 335     
Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
            340                 345                 350         
Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
        355                 360                 365             
Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
    370                 375                 380                 
Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
385                 390                 395                 400 
Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
                405                 410                 415     
Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
            420                 425                 430         
Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
        435                 440                 445             
Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Leu Glu Gly 
    450                 455                 460                 
Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
465                 470                 475                 480 
Asn Pro Gly Pro Arg Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys 
                485                 490                 495     
Glu Leu Pro His Pro Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn 
            500                 505                 510         
Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr 
        515                 520                 525             
Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His 
    530                 535                 540                 
Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro 
545                 550                 555                 560 
Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr 
                565                 570                 575     
Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His 
            580                 585                 590         
Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly 
        595                 600                 605             
Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu 
    610                 615                 620                 
Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn 
625                 630                 635                 640 
Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly 
                645                 650                 655     
Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser 
            660                 665                 670         
Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly 
        675                 680                 685             
Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser 
    690                 695                 700                 
Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro 
705                 710                 715                 720 
Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys 
                725                 730                 735     
Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn 
            740                 745                 750         
Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys Thr 
        755                 760                 765             
Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr 
    770                 775                 780                 
Ala Asp Ala Gly His Val Cys His Leu Cys His Pro Asn Cys Thr Tyr 
785                 790                 795                 800 
Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys 
                805                 810                 815     
Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu 
            820                 825                 830         
Val Val Ala Leu Gly Ile Gly Leu Phe Met 
        835                 840         

<210> 12
<211> 1048
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..25
<223> signal peptide

<220> 
<223> HLA-DRA*01:01 CLR long construct with Jun-Zipper

<220> 
<221> CHAIN
<222> 26..109
<223> alpha 1 domain

<220> 
<221> CHAIN
<222> 110..203
<223> alpha 2 domain

<220> 
<221> CHAIN
<222> 204..216
<223> extracellular part of transmembrane domain

<220> 
<221> CHAIN
<222> 217..223
<223> linker

<220> 
<221> CHAIN
<222> 224..257
<223> Jun-Zipper

<220> 
<221> CHAIN
<222> 258..485
<223> long spacer domain

<220> 
<221> CHAIN
<222> 486..513
<223> CD28 transmembrane domain

<220> 
<221> CHAIN
<222> 514..555
<223> 4-1BB costimulatory domain

<220> 
<221> CHAIN
<222> 556..667
<223> CD3 zeta signalling domain

<220> 
<221> CHAIN
<222> 668..691
<223> T2A element

<220> 
<221> CHAIN
<222> 692..713
<223> GM-CSF signal peptide

<220> 
<221> CHAIN
<222> 714..1048
<223> truncated EGFR selection domain

<400> 12
Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      
Leu Met Ser Ala Gln Glu Ser Trp Ala Ile Lys Glu Glu His Val Ile 
            20                  25                  30          
Ile Gln Ala Glu Phe Tyr Leu Asn Pro Asp Gln Ser Gly Glu Phe Met 
        35                  40                  45              
Phe Asp Phe Asp Gly Asp Glu Ile Phe His Val Asp Met Ala Lys Lys 
    50                  55                  60                  
Glu Thr Val Trp Arg Leu Glu Glu Phe Gly Arg Phe Ala Ser Phe Glu 
65                  70                  75                  80  
Ala Gln Gly Ala Leu Ala Asn Ile Ala Val Asp Lys Ala Asn Leu Glu 
                85                  90                  95      
Ile Met Thr Lys Arg Ser Asn Tyr Thr Pro Ile Thr Asn Val Pro Pro 
            100                 105                 110         
Glu Val Thr Val Leu Thr Asn Ser Pro Val Glu Leu Arg Glu Pro Asn 
        115                 120                 125             
Val Leu Ile Cys Phe Ile Asp Lys Phe Thr Pro Pro Val Val Asn Val 
    130                 135                 140                 
Thr Trp Leu Arg Asn Gly Lys Pro Val Thr Thr Gly Val Ser Glu Thr 
145                 150                 155                 160 
Val Phe Leu Pro Arg Glu Asp His Leu Phe Arg Lys Phe His Tyr Leu 
                165                 170                 175     
Pro Phe Leu Pro Ser Thr Glu Asp Val Tyr Asp Cys Arg Val Glu His 
            180                 185                 190         
Trp Gly Leu Asp Glu Pro Leu Leu Lys His Trp Glu Phe Asp Ala Pro 
        195                 200                 205             
Ser Pro Leu Pro Glu Thr Thr Glu Ser Arg Gly Gly Gly Gly Gly Ala 
    210                 215                 220                 
Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Glu Leu 
225                 230                 235                 240 
Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala Gln Leu Lys Gln 
                245                 250                 255     
Ser Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Pro 
            260                 265                 270         
Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
        275                 280                 285             
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
    290                 295                 300                 
Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
305                 310                 315                 320 
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser 
                325                 330                 335     
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
            340                 345                 350         
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
        355                 360                 365             
Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
    370                 375                 380                 
Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
385                 390                 395                 400 
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
                405                 410                 415     
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
            420                 425                 430         
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
        435                 440                 445             
Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
    450                 455                 460                 
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
465                 470                 475                 480 
Leu Ser Leu Gly Lys Met Phe Trp Val Leu Val Val Val Gly Gly Val 
                485                 490                 495     
Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
            500                 505                 510         
Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
        515                 520                 525             
Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
    530                 535                 540                 
Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser 
545                 550                 555                 560 
Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
                565                 570                 575     
Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
            580                 585                 590         
Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
        595                 600                 605             
Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
    610                 615                 620                 
Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
625                 630                 635                 640 
His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
                645                 650                 655     
Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Leu Glu Gly Gly Gly 
            660                 665                 670         
Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
        675                 680                 685             
Gly Pro Arg Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu 
    690                 695                 700                 
Pro His Pro Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile 
705                 710                 715                 720 
Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile 
                725                 730                 735     
Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu 
            740                 745                 750         
Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp 
        755                 760                 765             
Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe 
    770                 775                 780                 
Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe 
785                 790                 795                 800 
Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe 
                805                 810                 815     
Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser 
            820                 825                 830         
Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn 
        835                 840                 845             
Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser 
    850                 855                 860                 
Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys 
865                 870                 875                 880 
Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp 
                885                 890                 895     
Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly 
            900                 905                 910         
Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu 
        915                 920                 925             
Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro 
    930                 935                 940                 
Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile 
945                 950                 955                 960 
Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro 
                965                 970                 975     
Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp 
            980                 985                 990         
Ala Gly His Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys 
        995                 1000                1005            
Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro 
    1010                1015                1020                
Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val 
1025                1030                1035                1040
Ala Leu Gly Ile Gly Leu Phe Met 
                1045            

<210> 13
<211> 1058
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..29
<223> signal peptide

<220> 
<223> HLA-DRB1*01:01 CLR long construct with Fos-Zipper

<220> 
<221> CHAIN
<222> 30..123
<223> beta 1 domain

<220> 
<221> CHAIN
<222> 124..217
<223> beta 2 domain

<220> 
<221> CHAIN
<222> 218..227
<223> extracellular part of transmembrane domain

<220> 
<221> CHAIN
<222> 228..234
<223> linker

<220> 
<221> CHAIN
<222> 234..267
<223> Fos-Zipper

<220> 
<221> CHAIN
<222> 268..495
<223> long spacer domain

<220> 
<221> CHAIN
<222> 496..523
<223> CD28 transmembrane domain

<220> 
<221> CHAIN
<222> 524..565
<223> 4-1BB costimulatory domain

<220> 
<221> CHAIN
<222> 566..677
<223> CD3 zeta signalling domain

<220> 
<221> CHAIN
<222> 678..701
<223> T2A element

<220> 
<221> CHAIN
<222> 702..723
<223> GM-CSF signal peptide

<220> 
<221> CHAIN
<222> 724..1058
<223> truncated EGFR selection domain

<400> 13
Met Val Cys Leu Lys Leu Pro Gly Gly Ser Cys Met Thr Ala Leu Thr 
1               5                   10                  15      
Val Thr Leu Met Val Leu Ser Ser Pro Leu Ala Leu Ala Gly Asp Thr 
            20                  25                  30          
Arg Pro Arg Phe Leu Trp Gln Leu Lys Phe Glu Cys His Phe Phe Asn 
        35                  40                  45              
Gly Thr Glu Arg Val Arg Leu Leu Glu Arg Cys Ile Tyr Asn Gln Glu 
    50                  55                  60                  
Glu Ser Val Arg Phe Asp Ser Asp Val Gly Glu Tyr Arg Ala Val Thr 
65                  70                  75                  80  
Glu Leu Gly Arg Pro Asp Ala Glu Tyr Trp Asn Ser Gln Lys Asp Leu 
                85                  90                  95      
Leu Glu Gln Arg Arg Ala Ala Val Asp Thr Tyr Cys Arg His Asn Tyr 
            100                 105                 110         
Gly Val Gly Glu Ser Phe Thr Val Gln Arg Arg Val Glu Pro Lys Val 
        115                 120                 125             
Thr Val Tyr Pro Ser Lys Thr Gln Pro Leu Gln His His Asn Leu Leu 
    130                 135                 140                 
Val Cys Ser Val Ser Gly Phe Tyr Pro Gly Ser Ile Glu Val Arg Trp 
145                 150                 155                 160 
Phe Arg Asn Gly Gln Glu Glu Lys Ala Gly Val Val Ser Thr Gly Leu 
                165                 170                 175     
Ile Gln Asn Gly Asp Trp Thr Phe Gln Thr Leu Val Met Leu Glu Thr 
            180                 185                 190         
Val Pro Arg Ser Gly Glu Val Tyr Thr Cys Gln Val Glu His Pro Ser 
        195                 200                 205             
Val Thr Ser Pro Leu Thr Val Glu Trp Arg Ala Arg Ser Glu Ser Ala 
    210                 215                 220                 
Gln Ser Lys Ser Arg Gly Gly Gly Gly Gly Asp Thr Leu Gln Ala Glu 
225                 230                 235                 240 
Thr Asp Gln Leu Glu Asp Glu Lys Ser Ala Leu Gln Thr Glu Ile Ala 
                245                 250                 255     
Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe Glu Ser Lys Tyr Gly 
            260                 265                 270         
Pro Pro Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val 
        275                 280                 285             
Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
    290                 295                 300                 
Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu 
305                 310                 315                 320 
Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
                325                 330                 335     
Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser Thr Tyr Arg Val Val Ser 
            340                 345                 350         
Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
        355                 360                 365             
Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile 
    370                 375                 380                 
Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
385                 390                 395                 400 
Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
                405                 410                 415     
Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
            420                 425                 430         
Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
        435                 440                 445             
Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg 
    450                 455                 460                 
Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
465                 470                 475                 480 
His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys Met 
                485                 490                 495     
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
            500                 505                 510         
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys 
        515                 520                 525             
Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr 
    530                 535                 540                 
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu 
545                 550                 555                 560 
Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
                565                 570                 575     
Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
            580                 585                 590         
Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
        595                 600                 605             
Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
    610                 615                 620                 
Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
625                 630                 635                 640 
Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
                645                 650                 655     
Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
            660                 665                 670         
Ala Leu Pro Pro Arg Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu 
        675                 680                 685             
Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Arg Met Leu Leu 
    690                 695                 700                 
Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala Phe Leu 
705                 710                 715                 720 
Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys 
                725                 730                 735     
Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys 
            740                 745                 750         
Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly 
        755                 760                 765             
Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile 
    770                 775                 780                 
Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp 
785                 790                 795                 800 
Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile 
                805                 810                 815     
Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser 
            820                 825                 830         
Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp 
        835                 840                 845             
Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr 
    850                 855                 860                 
Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile 
865                 870                 875                 880 
Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys 
                885                 890                 895     
His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp 
            900                 905                 910         
Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys 
        915                 920                 925             
Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu 
    930                 935                 940                 
Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr 
945                 950                 955                 960 
Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile 
                965                 970                 975     
Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu 
            980                 985                 990         
Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His 
        995                 1000                1005            
Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu 
    1010                1015                1020                
Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met 
1025                1030                1035                1040
Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu 
                1045                1050                1055    
Phe Met 
        

<210> 14
<211> 832
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..25
<223> signal peptide

<220> 
<223> HLA-DRA*01:01 CLR short construct with Jun-Zipper

<220> 
<221> CHAIN
<222> 26..109
<223> alpha 1 chain

<220> 
<221> CHAIN
<222> 110..203
<223> alpha 2 chain

<220> 
<221> CHAIN
<222> 204..216
<223> extrcellular part of transmembrane domain

<220> 
<221> CHAIN
<222> 217..223
<223> linker

<220> 
<221> CHAIN
<222> 224..257
<223> Jun-Zipper

<220> 
<221> CHAIN
<222> 258..269
<223> short spacer domain

<220> 
<221> CHAIN
<222> 270..297
<223> CD28 transmembrane domain

<220> 
<221> CHAIN
<222> 298..339
<223> 4-1BB costimulatory domain

<220> 
<221> CHAIN
<222> 340..451
<223> CD3 zeta signalling domain

<220> 
<221> CHAIN
<222> 452..475
<223> T2A element

<220> 
<221> CHAIN
<222> 476..497
<223> GM-CSF signal peptide

<220> 
<221> CHAIN
<222> 498..832
<223> truncated EGFR selection domain

<400> 14
Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      
Leu Met Ser Ala Gln Glu Ser Trp Ala Ile Lys Glu Glu His Val Ile 
            20                  25                  30          
Ile Gln Ala Glu Phe Tyr Leu Asn Pro Asp Gln Ser Gly Glu Phe Met 
        35                  40                  45              
Phe Asp Phe Asp Gly Asp Glu Ile Phe His Val Asp Met Ala Lys Lys 
    50                  55                  60                  
Glu Thr Val Trp Arg Leu Glu Glu Phe Gly Arg Phe Ala Ser Phe Glu 
65                  70                  75                  80  
Ala Gln Gly Ala Leu Ala Asn Ile Ala Val Asp Lys Ala Asn Leu Glu 
                85                  90                  95      
Ile Met Thr Lys Arg Ser Asn Tyr Thr Pro Ile Thr Asn Val Pro Pro 
            100                 105                 110         
Glu Val Thr Val Leu Thr Asn Ser Pro Val Glu Leu Arg Glu Pro Asn 
        115                 120                 125             
Val Leu Ile Cys Phe Ile Asp Lys Phe Thr Pro Pro Val Val Asn Val 
    130                 135                 140                 
Thr Trp Leu Arg Asn Gly Lys Pro Val Thr Thr Gly Val Ser Glu Thr 
145                 150                 155                 160 
Val Phe Leu Pro Arg Glu Asp His Leu Phe Arg Lys Phe His Tyr Leu 
                165                 170                 175     
Pro Phe Leu Pro Ser Thr Glu Asp Val Tyr Asp Cys Arg Val Glu His 
            180                 185                 190         
Trp Gly Leu Asp Glu Pro Leu Leu Lys His Trp Glu Phe Asp Ala Pro 
        195                 200                 205             
Ser Pro Leu Pro Glu Thr Thr Glu Ser Arg Gly Gly Gly Gly Gly Ala 
    210                 215                 220                 
Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Glu Leu 
225                 230                 235                 240 
Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala Gln Leu Lys Gln 
                245                 250                 255     
Ser Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Met Phe Trp 
            260                 265                 270         
Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
        275                 280                 285             
Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys Leu 
    290                 295                 300                 
Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln 
305                 310                 315                 320 
Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly 
                325                 330                 335     
Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
            340                 345                 350         
Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
        355                 360                 365             
Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
    370                 375                 380                 
Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
385                 390                 395                 400 
Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
                405                 410                 415     
Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
            420                 425                 430         
Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
        435                 440                 445             
Pro Pro Arg Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr 
    450                 455                 460                 
Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Arg Met Leu Leu Leu Val 
465                 470                 475                 480 
Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala Phe Leu Leu Ile 
                485                 490                 495     
Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser 
            500                 505                 510         
Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser 
        515                 520                 525             
Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser 
    530                 535                 540                 
Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys 
545                 550                 555                 560 
Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu 
                565                 570                 575     
Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly 
            580                 585                 590         
Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn 
        595                 600                 605             
Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp 
    610                 615                 620                 
Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn 
625                 630                 635                 640 
Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser 
                645                 650                 655     
Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala 
            660                 665                 670         
Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val 
        675                 680                 685             
Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn 
    690                 695                 700                 
Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile 
705                 710                 715                 720 
Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr 
                725                 730                 735     
Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly 
            740                 745                 750         
Pro His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn 
        755                 760                 765             
Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys 
    770                 775                 780                 
His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys 
785                 790                 795                 800 
Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly 
                805                 810                 815     
Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met 
            820                 825                 830         



<210> 15
<211> 267
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..29
<223> signal peptide

<220> 
<223> HLA-DRB1*01:01 ligand with linker and Fos-Zipper

<220> 
<221> CHAIN
<222> 30..123
<223> beta 1 domain

<220> 
<221> CHAIN
<222> 124..217
<223> beta 2 domain

<220> 
<221> CHAIN
<222> 218..227
<223> extracellular part of transmembrane domain

<220> 
<221> CHAIN
<222> 228..234
<223> linker

<220> 
<221> CHAIN
<222> 235..267
<223> Fos-Zipper

<400> 15
Met Val Cys Leu Lys Leu Pro Gly Gly Ser Cys Met Thr Ala Leu Thr 
1               5                   10                  15      
Val Thr Leu Met Val Leu Ser Ser Pro Leu Ala Leu Ala Gly Asp Thr 
            20                  25                  30          
Arg Pro Arg Phe Leu Trp Gln Leu Lys Phe Glu Cys His Phe Phe Asn 
        35                  40                  45              
Gly Thr Glu Arg Val Arg Leu Leu Glu Arg Cys Ile Tyr Asn Gln Glu 
    50                  55                  60                  
Glu Ser Val Arg Phe Asp Ser Asp Val Gly Glu Tyr Arg Ala Val Thr 
65                  70                  75                  80  
Glu Leu Gly Arg Pro Asp Ala Glu Tyr Trp Asn Ser Gln Lys Asp Leu 
                85                  90                  95      
Leu Glu Gln Arg Arg Ala Ala Val Asp Thr Tyr Cys Arg His Asn Tyr 
            100                 105                 110         
Gly Val Gly Glu Ser Phe Thr Val Gln Arg Arg Val Glu Pro Lys Val 
        115                 120                 125             
Thr Val Tyr Pro Ser Lys Thr Gln Pro Leu Gln His His Asn Leu Leu 
    130                 135                 140                 
Val Cys Ser Val Ser Gly Phe Tyr Pro Gly Ser Ile Glu Val Arg Trp 
145                 150                 155                 160 
Phe Arg Asn Gly Gln Glu Glu Lys Ala Gly Val Val Ser Thr Gly Leu 
                165                 170                 175     
Ile Gln Asn Gly Asp Trp Thr Phe Gln Thr Leu Val Met Leu Glu Thr 
            180                 185                 190         
Val Pro Arg Ser Gly Glu Val Tyr Thr Cys Gln Val Glu His Pro Ser 
        195                 200                 205             
Val Thr Ser Pro Leu Thr Val Glu Trp Arg Ala Arg Ser Glu Ser Ala 
    210                 215                 220                 
Gln Ser Lys Ser Arg Gly Gly Gly Gly Gly Asp Thr Leu Gln Ala Glu 
225                 230                 235                 240 
Thr Asp Gln Leu Glu Asp Glu Lys Ser Ala Leu Gln Thr Glu Ile Ala 
                245                 250                 255     
Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe 
            260                 265         

<210> 16
<211> 1048
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..25
<223> signal peptide

<220> 
<223> HLA-DRA*01:01 CLR long construct with Jun-Zipper

<220> 
<221> CHAIN
<222> 26..109
<223> alpha 1 domain

<220> 
<221> CHAIN
<222> 110..203
<223> alpha 2 domain

<220> 
<221> CHAIN
<222> 204..216
<223> extracellular part of transmembrane domain

<220> 
<221> CHAIN
<222> 217..223
<223> linker

<220> 
<221> CHAIN
<222> 224..257
<223> Jun-Zipper

<220> 
<221> CHAIN
<222> 258..485
<223> long spacer domain

<220> 
<221> CHAIN
<222> 486..513
<223> CD28 transmembrane domain

<220> 
<221> CHAIN
<222> 514..555
<223> 4-1BB costimulatory domain

<220> 
<221> CHAIN
<222> 556..667
<223> CD3 zeta signalling domain

<220> 
<221> CHAIN
<222> 668..691
<223> T2A element

<220> 
<221> CHAIN
<222> 692..713
<223> GM-CSF signal peptied

<220> 
<221> CHAIN
<222> 714..1048
<223> EGFR selection domain

<400> 16
Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      
Leu Met Ser Ala Gln Glu Ser Trp Ala Ile Lys Glu Glu His Val Ile 
            20                  25                  30          
Ile Gln Ala Glu Phe Tyr Leu Asn Pro Asp Gln Ser Gly Glu Phe Met 
        35                  40                  45              
Phe Asp Phe Asp Gly Asp Glu Ile Phe His Val Asp Met Ala Lys Lys 
    50                  55                  60                  
Glu Thr Val Trp Arg Leu Glu Glu Phe Gly Arg Phe Ala Ser Phe Glu 
65                  70                  75                  80  
Ala Gln Gly Ala Leu Ala Asn Ile Ala Val Asp Lys Ala Asn Leu Glu 
                85                  90                  95      
Ile Met Thr Lys Arg Ser Asn Tyr Thr Pro Ile Thr Asn Val Pro Pro 
            100                 105                 110         
Glu Val Thr Val Leu Thr Asn Ser Pro Val Glu Leu Arg Glu Pro Asn 
        115                 120                 125             
Val Leu Ile Cys Phe Ile Asp Lys Phe Thr Pro Pro Val Val Asn Val 
    130                 135                 140                 
Thr Trp Leu Arg Asn Gly Lys Pro Val Thr Thr Gly Val Ser Glu Thr 
145                 150                 155                 160 
Val Phe Leu Pro Arg Glu Asp His Leu Phe Arg Lys Phe His Tyr Leu 
                165                 170                 175     
Pro Phe Leu Pro Ser Thr Glu Asp Val Tyr Asp Cys Arg Val Glu His 
            180                 185                 190         
Trp Gly Leu Asp Glu Pro Leu Leu Lys His Trp Glu Phe Asp Ala Pro 
        195                 200                 205             
Ser Pro Leu Pro Glu Thr Thr Glu Ser Arg Gly Gly Gly Gly Gly Ala 
    210                 215                 220                 
Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Glu Leu 
225                 230                 235                 240 
Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala Gln Leu Lys Gln 
                245                 250                 255     
Ser Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Pro 
            260                 265                 270         
Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
        275                 280                 285             
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
    290                 295                 300                 
Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
305                 310                 315                 320 
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser 
                325                 330                 335     
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
            340                 345                 350         
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
        355                 360                 365             
Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
    370                 375                 380                 
Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
385                 390                 395                 400 
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
                405                 410                 415     
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
            420                 425                 430         
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
        435                 440                 445             
Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
    450                 455                 460                 
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
465                 470                 475                 480 
Leu Ser Leu Gly Lys Met Phe Trp Val Leu Val Val Val Gly Gly Val 
                485                 490                 495     
Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
            500                 505                 510         
Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
        515                 520                 525             
Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
    530                 535                 540                 
Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser 
545                 550                 555                 560 
Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
                565                 570                 575     
Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
            580                 585                 590         
Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
        595                 600                 605             
Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
    610                 615                 620                 
Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
625                 630                 635                 640 
His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
                645                 650                 655     
Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Leu Glu Gly Gly Gly 
            660                 665                 670         
Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
        675                 680                 685             
Gly Pro Arg Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu 
    690                 695                 700                 
Pro His Pro Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile 
705                 710                 715                 720 
Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile 
                725                 730                 735     
Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu 
            740                 745                 750         
Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp 
        755                 760                 765             
Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe 
    770                 775                 780                 
Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe 
785                 790                 795                 800 
Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe 
                805                 810                 815     
Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser 
            820                 825                 830         
Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn 
        835                 840                 845             
Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser 
    850                 855                 860                 
Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys 
865                 870                 875                 880 
Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp 
                885                 890                 895     
Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly 
            900                 905                 910         
Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu 
        915                 920                 925             
Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro 
    930                 935                 940                 
Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile 
945                 950                 955                 960 
Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro 
                965                 970                 975     
Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp 
            980                 985                 990         
Ala Gly His Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys 
        995                 1000                1005            
Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro 
    1010                1015                1020                
Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val 
1025                1030                1035                1040
Ala Leu Gly Ile Gly Leu Phe Met 
                1045            

<210> 17
<211> 267
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..29
<223> signal peptide

<220> 
<223> HLA-DRB1*01:01 ligand with linker and Fos-Zipper

<220> 
<221> CHAIN
<222> 30..123
<223> beta 1 domain

<220> 
<221> CHAIN
<222> 124..217
<223> beta 2 domain

<220> 
<221> CHAIN
<222> 218..227
<223> extracellular part of transmembrane domain

<220> 
<221> CHAIN
<222> 228..234
<223> linker

<220> 
<221> CHAIN
<222> 235..267
<223> Fos-Zipper

<400> 17
Met Val Cys Leu Lys Leu Pro Gly Gly Ser Cys Met Thr Ala Leu Thr 
1               5                   10                  15      
Val Thr Leu Met Val Leu Ser Ser Pro Leu Ala Leu Ala Gly Asp Thr 
            20                  25                  30          
Arg Pro Arg Phe Leu Trp Gln Leu Lys Phe Glu Cys His Phe Phe Asn 
        35                  40                  45              
Gly Thr Glu Arg Val Arg Leu Leu Glu Arg Cys Ile Tyr Asn Gln Glu 
    50                  55                  60                  
Glu Ser Val Arg Phe Asp Ser Asp Val Gly Glu Tyr Arg Ala Val Thr 
65                  70                  75                  80  
Glu Leu Gly Arg Pro Asp Ala Glu Tyr Trp Asn Ser Gln Lys Asp Leu 
                85                  90                  95      
Leu Glu Gln Arg Arg Ala Ala Val Asp Thr Tyr Cys Arg His Asn Tyr 
            100                 105                 110         
Gly Val Gly Glu Ser Phe Thr Val Gln Arg Arg Val Glu Pro Lys Val 
        115                 120                 125             
Thr Val Tyr Pro Ser Lys Thr Gln Pro Leu Gln His His Asn Leu Leu 
    130                 135                 140                 
Val Cys Ser Val Ser Gly Phe Tyr Pro Gly Ser Ile Glu Val Arg Trp 
145                 150                 155                 160 
Phe Arg Asn Gly Gln Glu Glu Lys Ala Gly Val Val Ser Thr Gly Leu 
                165                 170                 175     
Ile Gln Asn Gly Asp Trp Thr Phe Gln Thr Leu Val Met Leu Glu Thr 
            180                 185                 190         
Val Pro Arg Ser Gly Glu Val Tyr Thr Cys Gln Val Glu His Pro Ser 
        195                 200                 205             
Val Thr Ser Pro Leu Thr Val Glu Trp Arg Ala Arg Ser Glu Ser Ala 
    210                 215                 220                 
Gln Ser Lys Ser Arg Gly Gly Gly Gly Gly Asp Thr Leu Gln Ala Glu 
225                 230                 235                 240 
Thr Asp Gln Leu Glu Asp Glu Lys Ser Ala Leu Gln Thr Glu Ile Ala 
                245                 250                 255     
Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe 
            260                 265         

<210> 18
<211> 842
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..29
<223> signal peptide

<220> 
<223> HLA-DRB1*01:01 CLR short construct with Fos-Zipper

<220> 
<221> CHAIN
<222> 30..123
<223> beta 1 domain

<220> 
<221> CHAIN
<222> 124..217
<223> beta 2 domain

<220> 
<221> CHAIN
<222> 218..227
<223> extracellular part of transmembrane domain

<220> 
<221> CHAIN
<222> 228..234
<223> linker

<220> 
<221> CHAIN
<222> 235..267
<223> Fos-Zipper

<220> 
<221> CHAIN
<222> 268..279
<223> short spacer domain

<220> 
<221> CHAIN
<222> 280..307
<223> CD28 transmembrane domain

<220> 
<221> CHAIN
<222> 308..349
<223> 4-1BB costimulatory domain

<220> 
<221> CHAIN
<222> 350..461
<223> CD3 zeta signalling domain

<220> 
<221> CHAIN
<222> 462..485
<223> T2A element

<220> 
<221> CHAIN
<222> 486..507
<223> GM-CSF signal peptide

<220> 
<221> CHAIN
<222> 508..842
<223> truncated EGFR selection domain

<400> 18
Met Val Cys Leu Lys Leu Pro Gly Gly Ser Cys Met Thr Ala Leu Thr 
1               5                   10                  15      
Val Thr Leu Met Val Leu Ser Ser Pro Leu Ala Leu Ala Gly Asp Thr 
            20                  25                  30          
Arg Pro Arg Phe Leu Trp Gln Leu Lys Phe Glu Cys His Phe Phe Asn 
        35                  40                  45              
Gly Thr Glu Arg Val Arg Leu Leu Glu Arg Cys Ile Tyr Asn Gln Glu 
    50                  55                  60                  
Glu Ser Val Arg Phe Asp Ser Asp Val Gly Glu Tyr Arg Ala Val Thr 
65                  70                  75                  80  
Glu Leu Gly Arg Pro Asp Ala Glu Tyr Trp Asn Ser Gln Lys Asp Leu 
                85                  90                  95      
Leu Glu Gln Arg Arg Ala Ala Val Asp Thr Tyr Cys Arg His Asn Tyr 
            100                 105                 110         
Gly Val Gly Glu Ser Phe Thr Val Gln Arg Arg Val Glu Pro Lys Val 
        115                 120                 125             
Thr Val Tyr Pro Ser Lys Thr Gln Pro Leu Gln His His Asn Leu Leu 
    130                 135                 140                 
Val Cys Ser Val Ser Gly Phe Tyr Pro Gly Ser Ile Glu Val Arg Trp 
145                 150                 155                 160 
Phe Arg Asn Gly Gln Glu Glu Lys Ala Gly Val Val Ser Thr Gly Leu 
                165                 170                 175     
Ile Gln Asn Gly Asp Trp Thr Phe Gln Thr Leu Val Met Leu Glu Thr 
            180                 185                 190         
Val Pro Arg Ser Gly Glu Val Tyr Thr Cys Gln Val Glu His Pro Ser 
        195                 200                 205             
Val Thr Ser Pro Leu Thr Val Glu Trp Arg Ala Arg Ser Glu Ser Ala 
    210                 215                 220                 
Gln Ser Lys Ser Arg Gly Gly Gly Gly Gly Asp Thr Leu Gln Ala Glu 
225                 230                 235                 240 
Thr Asp Gln Leu Glu Asp Glu Lys Ser Ala Leu Gln Thr Glu Ile Ala 
                245                 250                 255     
Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe Glu Ser Lys Tyr Gly 
            260                 265                 270         
Pro Pro Cys Pro Pro Cys Pro Met Phe Trp Val Leu Val Val Val Gly 
        275                 280                 285             
Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
    290                 295                 300                 
Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
305                 310                 315                 320 
Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
                325                 330                 335     
Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
            340                 345                 350         
Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
        355                 360                 365             
Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
    370                 375                 380                 
Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
385                 390                 395                 400 
Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
                405                 410                 415     
Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
            420                 425                 430         
Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
        435                 440                 445             
Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Leu Glu Gly 
    450                 455                 460                 
Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
465                 470                 475                 480 
Asn Pro Gly Pro Arg Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys 
                485                 490                 495     
Glu Leu Pro His Pro Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn 
            500                 505                 510         
Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr 
        515                 520                 525             
Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His 
    530                 535                 540                 
Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro 
545                 550                 555                 560 
Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr 
                565                 570                 575     
Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His 
            580                 585                 590         
Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly 
        595                 600                 605             
Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu 
    610                 615                 620                 
Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn 
625                 630                 635                 640 
Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly 
                645                 650                 655     
Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser 
            660                 665                 670         
Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly 
        675                 680                 685             
Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser 
    690                 695                 700                 
Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro 
705                 710                 715                 720 
Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys 
                725                 730                 735     
Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn 
            740                 745                 750         
Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys Thr 
        755                 760                 765             
Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr 
    770                 775                 780                 
Ala Asp Ala Gly His Val Cys His Leu Cys His Pro Asn Cys Thr Tyr 
785                 790                 795                 800 
Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys 
                805                 810                 815     
Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu 
            820                 825                 830         
Val Val Ala Leu Gly Ile Gly Leu Phe Met 
        835                 840         

<210> 19
<211> 257
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..25
<223> signal peptide

<220> 
<223> HLA-DRA*01:01 ligand with linker and Jun-Zipper

<220> 
<221> CHAIN
<222> 26..109
<223> alpha 1 chain

<220> 
<221> CHAIN
<222> 110..203
<223> alpha 2 chain

<220> 
<221> CHAIN
<222> 204..216
<223> extracellular part of transmembrane domain

<220> 
<221> CHAIN
<222> 217..223
<223> linker

<220> 
<221> CHAIN
<222> 224..257
<223> Jun-Zipper

<400> 19
Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      
Leu Met Ser Ala Gln Glu Ser Trp Ala Ile Lys Glu Glu His Val Ile 
            20                  25                  30          
Ile Gln Ala Glu Phe Tyr Leu Asn Pro Asp Gln Ser Gly Glu Phe Met 
        35                  40                  45              
Phe Asp Phe Asp Gly Asp Glu Ile Phe His Val Asp Met Ala Lys Lys 
    50                  55                  60                  
Glu Thr Val Trp Arg Leu Glu Glu Phe Gly Arg Phe Ala Ser Phe Glu 
65                  70                  75                  80  
Ala Gln Gly Ala Leu Ala Asn Ile Ala Val Asp Lys Ala Asn Leu Glu 
                85                  90                  95      
Ile Met Thr Lys Arg Ser Asn Tyr Thr Pro Ile Thr Asn Val Pro Pro 
            100                 105                 110         
Glu Val Thr Val Leu Thr Asn Ser Pro Val Glu Leu Arg Glu Pro Asn 
        115                 120                 125             
Val Leu Ile Cys Phe Ile Asp Lys Phe Thr Pro Pro Val Val Asn Val 
    130                 135                 140                 
Thr Trp Leu Arg Asn Gly Lys Pro Val Thr Thr Gly Val Ser Glu Thr 
145                 150                 155                 160 
Val Phe Leu Pro Arg Glu Asp His Leu Phe Arg Lys Phe His Tyr Leu 
                165                 170                 175     
Pro Phe Leu Pro Ser Thr Glu Asp Val Tyr Asp Cys Arg Val Glu His 
            180                 185                 190         
Trp Gly Leu Asp Glu Pro Leu Leu Lys His Trp Glu Phe Asp Ala Pro 
        195                 200                 205             
Ser Pro Leu Pro Glu Thr Thr Glu Ser Arg Gly Gly Gly Gly Gly Ala 
    210                 215                 220                 
Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Glu Leu 
225                 230                 235                 240 
Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala Gln Leu Lys Gln 
                245                 250                 255     
Ser 
    

<210> 20
<211> 1058
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..29
<223> signal peptide

<220> 
<223> HLA-DRB1*01:01 CLR long construct with Fos-Zipper

<220> 
<221> CHAIN
<222> 30..123
<223> beta 1 domain

<220> 
<221> CHAIN
<222> 124..217
<223> beta 2 domain

<220> 
<221> CHAIN
<222> 218..227
<223> extracellular part of transmembrane domain

<220> 
<221> CHAIN
<222> 228..234
<223> linker

<220> 
<221> CHAIN
<222> 235..267
<223> Fos-Zipper

<220> 
<221> CHAIN
<222> 268..495
<223> long spacer domain

<220> 
<221> CHAIN
<222> 496..523
<223> CD28 transmembrane domain

<220> 
<221> CHAIN
<222> 524..565
<223> 4-1BB costimulatory domain

<220> 
<221> CHAIN
<222> 566..677
<223> CD3 zeta signalling domain

<220> 
<221> CHAIN
<222> 678..701
<223> T2A element

<220> 
<221> CHAIN
<222> 702..723
<223> GM-CSF signal peptide

<220> 
<221> CHAIN
<222> 724..1058
<223> EGFR selection domain

<400> 20
Met Val Cys Leu Lys Leu Pro Gly Gly Ser Cys Met Thr Ala Leu Thr 
1               5                   10                  15      
Val Thr Leu Met Val Leu Ser Ser Pro Leu Ala Leu Ala Gly Asp Thr 
            20                  25                  30          
Arg Pro Arg Phe Leu Trp Gln Leu Lys Phe Glu Cys His Phe Phe Asn 
        35                  40                  45              
Gly Thr Glu Arg Val Arg Leu Leu Glu Arg Cys Ile Tyr Asn Gln Glu 
    50                  55                  60                  
Glu Ser Val Arg Phe Asp Ser Asp Val Gly Glu Tyr Arg Ala Val Thr 
65                  70                  75                  80  
Glu Leu Gly Arg Pro Asp Ala Glu Tyr Trp Asn Ser Gln Lys Asp Leu 
                85                  90                  95      
Leu Glu Gln Arg Arg Ala Ala Val Asp Thr Tyr Cys Arg His Asn Tyr 
            100                 105                 110         
Gly Val Gly Glu Ser Phe Thr Val Gln Arg Arg Val Glu Pro Lys Val 
        115                 120                 125             
Thr Val Tyr Pro Ser Lys Thr Gln Pro Leu Gln His His Asn Leu Leu 
    130                 135                 140                 
Val Cys Ser Val Ser Gly Phe Tyr Pro Gly Ser Ile Glu Val Arg Trp 
145                 150                 155                 160 
Phe Arg Asn Gly Gln Glu Glu Lys Ala Gly Val Val Ser Thr Gly Leu 
                165                 170                 175     
Ile Gln Asn Gly Asp Trp Thr Phe Gln Thr Leu Val Met Leu Glu Thr 
            180                 185                 190         
Val Pro Arg Ser Gly Glu Val Tyr Thr Cys Gln Val Glu His Pro Ser 
        195                 200                 205             
Val Thr Ser Pro Leu Thr Val Glu Trp Arg Ala Arg Ser Glu Ser Ala 
    210                 215                 220                 
Gln Ser Lys Ser Arg Gly Gly Gly Gly Gly Asp Thr Leu Gln Ala Glu 
225                 230                 235                 240 
Thr Asp Gln Leu Glu Asp Glu Lys Ser Ala Leu Gln Thr Glu Ile Ala 
                245                 250                 255     
Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe Glu Ser Lys Tyr Gly 
            260                 265                 270         
Pro Pro Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val 
        275                 280                 285             
Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
    290                 295                 300                 
Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu 
305                 310                 315                 320 
Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
                325                 330                 335     
Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser Thr Tyr Arg Val Val Ser 
            340                 345                 350         
Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
        355                 360                 365             
Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile 
    370                 375                 380                 
Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
385                 390                 395                 400 
Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
                405                 410                 415     
Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
            420                 425                 430         
Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
        435                 440                 445             
Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg 
    450                 455                 460                 
Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
465                 470                 475                 480 
His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys Met 
                485                 490                 495     
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
            500                 505                 510         
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys 
        515                 520                 525             
Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr 
    530                 535                 540                 
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu 
545                 550                 555                 560 
Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
                565                 570                 575     
Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
            580                 585                 590         
Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
        595                 600                 605             
Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
    610                 615                 620                 
Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
625                 630                 635                 640 
Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
                645                 650                 655     
Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
            660                 665                 670         
Ala Leu Pro Pro Arg Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu 
        675                 680                 685             
Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Arg Met Leu Leu 
    690                 695                 700                 
Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala Phe Leu 
705                 710                 715                 720 
Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys 
                725                 730                 735     
Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys 
            740                 745                 750         
Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly 
        755                 760                 765             
Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile 
    770                 775                 780                 
Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp 
785                 790                 795                 800 
Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile 
                805                 810                 815     
Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser 
            820                 825                 830         
Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp 
        835                 840                 845             
Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr 
    850                 855                 860                 
Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile 
865                 870                 875                 880 
Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys 
                885                 890                 895     
His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp 
            900                 905                 910         
Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys 
        915                 920                 925             
Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu 
    930                 935                 940                 
Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr 
945                 950                 955                 960 
Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile 
                965                 970                 975     
Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu 
            980                 985                 990         
Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His 
        995                 1000                1005            
Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu 
    1010                1015                1020                
Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met 
1025                1030                1035                1040
Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu 
                1045                1050                1055    
Phe Met 
        

<210> 21
<211> 257
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..25
<223> signal peptide

<220> 
<223> HLA-DRA*01:01 Ligand with linker and Jun-Zipper

<220> 
<221> CHAIN
<222> 26..109
<223> alpha 1 domain

<220> 
<221> CHAIN
<222> 110..203
<223> alpha 2 domain

<220> 
<221> CHAIN
<222> 204..216
<223> extracellular part of transmembrane domain

<220> 
<221> CHAIN
<222> 217..223
<223> linker

<220> 
<221> CHAIN
<222> 224..257
<223> Jun-Zipper

<400> 21
Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      
Leu Met Ser Ala Gln Glu Ser Trp Ala Ile Lys Glu Glu His Val Ile 
            20                  25                  30          
Ile Gln Ala Glu Phe Tyr Leu Asn Pro Asp Gln Ser Gly Glu Phe Met 
        35                  40                  45              
Phe Asp Phe Asp Gly Asp Glu Ile Phe His Val Asp Met Ala Lys Lys 
    50                  55                  60                  
Glu Thr Val Trp Arg Leu Glu Glu Phe Gly Arg Phe Ala Ser Phe Glu 
65                  70                  75                  80  
Ala Gln Gly Ala Leu Ala Asn Ile Ala Val Asp Lys Ala Asn Leu Glu 
                85                  90                  95      
Ile Met Thr Lys Arg Ser Asn Tyr Thr Pro Ile Thr Asn Val Pro Pro 
            100                 105                 110         
Glu Val Thr Val Leu Thr Asn Ser Pro Val Glu Leu Arg Glu Pro Asn 
        115                 120                 125             
Val Leu Ile Cys Phe Ile Asp Lys Phe Thr Pro Pro Val Val Asn Val 
    130                 135                 140                 
Thr Trp Leu Arg Asn Gly Lys Pro Val Thr Thr Gly Val Ser Glu Thr 
145                 150                 155                 160 
Val Phe Leu Pro Arg Glu Asp His Leu Phe Arg Lys Phe His Tyr Leu 
                165                 170                 175     
Pro Phe Leu Pro Ser Thr Glu Asp Val Tyr Asp Cys Arg Val Glu His 
            180                 185                 190         
Trp Gly Leu Asp Glu Pro Leu Leu Lys His Trp Glu Phe Asp Ala Pro 
        195                 200                 205             
Ser Pro Leu Pro Glu Thr Thr Glu Ser Arg Gly Gly Gly Gly Gly Ala 
    210                 215                 220                 
Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Glu Leu 
225                 230                 235                 240 
Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala Gln Leu Lys Gln 
                245                 250                 255     
Ser 
    

