                         SEQUENCE LISTING

<110>  Commonwealth Scientific and Industrial Research 
       Organisation
 
<120>  Immunosensors

<130>  529697PCT

<150>  AU2019901483
<151>  2019-05-01

<150>  AU2019901566
<151>  2019-05-08

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  1

Trp Ala Leu Gln Arg Pro His 
1               5           


<210>  2
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  2

Trp Glu Leu Gln Arg Pro Tyr 
1               5           


<210>  3
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker


<220>
<221>  X
<222>  (3)..(3)
<223>  X is any amino acid

<400>  3

Leu Pro Xaa Thr 
1               


<210>  4
<211>  365
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLAG-GFP2

<400>  4

Met Gly His His His His His His Gly Thr Glu Asn Leu Tyr Phe Gln 
1               5                   10                  15      


Ser Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys 
            20                  25                  30          


Pro Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly 
        35                  40                  45              


Ser Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg 
    50                  55                  60                  


Leu Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu 
65                  70                  75                  80  


Arg Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu 
                85                  90                  95      


Asp Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln 
            100                 105                 110         


Ile Gly Gly Gly Ser Asp Tyr Lys Asp Asp Asp Asp Lys Gly Ser Val 
        115                 120                 125             


Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu 
    130                 135                 140                 


Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly 
145                 150                 155                 160 


Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr 
                165                 170                 175     


Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Ser 
            180                 185                 190         


Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His 
        195                 200                 205             


Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr 
    210                 215                 220                 


Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys 
225                 230                 235                 240 


Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp 
                245                 250                 255     


Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr 
            260                 265                 270         


Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile 
        275                 280                 285             


Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln 
    290                 295                 300                 


Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val 
305                 310                 315                 320 


Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys 
                325                 330                 335     


Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr 
            340                 345                 350         


Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
        355                 360                 365 


<210>  5
<211>  340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-His-RLuc8

<400>  5

Met Gly His His His His His His Gly Thr Glu Asn Leu Tyr Phe Gln 
1               5                   10                  15      


Gly Ser Ala Ser Lys Val Tyr Asp Pro Glu Gln Arg Lys Arg Met Ile 
            20                  25                  30          


Thr Gly Pro Gln Trp Trp Ala Arg Cys Lys Gln Met Asn Val Leu Asp 
        35                  40                  45              


Ser Phe Ile Asn Tyr Tyr Asp Ser Glu Lys His Ala Glu Asn Ala Val 
    50                  55                  60                  


Ile Phe Leu His Gly Asn Ala Thr Ser Ser Tyr Leu Trp Arg His Val 
65                  70                  75                  80  


Val Pro His Ile Glu Pro Val Ala Arg Cys Ile Ile Pro Asp Leu Ile 
                85                  90                  95      


Gly Met Gly Lys Ser Gly Lys Ser Gly Asn Gly Ser Tyr Arg Leu Leu 
            100                 105                 110         


Asp His Tyr Lys Tyr Leu Thr Ala Trp Phe Glu Leu Leu Asn Leu Pro 
        115                 120                 125             


Lys Lys Ile Ile Phe Val Gly His Asp Trp Gly Ala Ala Leu Ala Phe 
    130                 135                 140                 


His Tyr Ala Tyr Glu His Gln Asp Arg Ile Lys Ala Ile Val His Met 
145                 150                 155                 160 


Glu Ser Val Val Asp Val Ile Glu Ser Trp Asp Glu Trp Pro Asp Ile 
                165                 170                 175     


Glu Glu Asp Ile Ala Leu Ile Lys Ser Glu Glu Gly Glu Lys Met Val 
            180                 185                 190         


Leu Glu Asn Asn Phe Phe Val Glu Thr Val Leu Pro Ser Lys Ile Met 
        195                 200                 205             


Arg Lys Leu Glu Pro Glu Glu Phe Ala Ala Tyr Leu Glu Pro Phe Lys 
    210                 215                 220                 


Glu Lys Gly Glu Val Arg Arg Pro Thr Leu Ser Trp Pro Arg Glu Ile 
225                 230                 235                 240 


Pro Leu Val Lys Gly Gly Lys Pro Asp Val Val Gln Ile Val Arg Asn 
                245                 250                 255     


Tyr Asn Ala Tyr Leu Arg Ala Ser Asp Asp Leu Pro Lys Leu Phe Ile 
            260                 265                 270         


Glu Ser Asp Pro Gly Phe Phe Ser Asn Ala Ile Val Glu Gly Ala Lys 
        275                 280                 285             


Lys Phe Pro Asn Thr Glu Phe Val Lys Val Lys Gly Leu His Phe Leu 
    290                 295                 300                 


Gln Glu Asp Ala Pro Asp Glu Met Gly Lys Tyr Ile Lys Ser Phe Val 
305                 310                 315                 320 


Glu Arg Val Leu Lys Asn Glu Gln Leu Pro Glu Thr Gly Gly Ala Ala 
                325                 330                 335     


Leu Glu Ala Ser 
            340 


<210>  6
<211>  449
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLAG-RLuc8

<400>  6

Met Gly His His His His His His Gly Thr Glu Asn Leu Tyr Phe Gln 
1               5                   10                  15      


Ser Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys 
            20                  25                  30          


Pro Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly 
        35                  40                  45              


Ser Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg 
    50                  55                  60                  


Leu Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu 
65                  70                  75                  80  


Arg Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu 
                85                  90                  95      


Asp Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln 
            100                 105                 110         


Ile Gly Gly Gly Ser Asp Tyr Lys Asp Asp Asp Asp Lys Gly Ser Ala 
        115                 120                 125             


Ser Lys Val Tyr Asp Pro Glu Gln Arg Lys Arg Met Ile Thr Gly Pro 
    130                 135                 140                 


Gln Trp Trp Ala Arg Cys Lys Gln Met Asn Val Leu Asp Ser Phe Ile 
145                 150                 155                 160 


Asn Tyr Tyr Asp Ser Glu Lys His Ala Glu Asn Ala Val Ile Phe Leu 
                165                 170                 175     


His Gly Asn Ala Thr Ser Ser Tyr Leu Trp Arg His Val Val Pro His 
            180                 185                 190         


Ile Glu Pro Val Ala Arg Cys Ile Ile Pro Asp Leu Ile Gly Met Gly 
        195                 200                 205             


Lys Ser Gly Lys Ser Gly Asn Gly Ser Tyr Arg Leu Leu Asp His Tyr 
    210                 215                 220                 


Lys Tyr Leu Thr Ala Trp Phe Glu Leu Leu Asn Leu Pro Lys Lys Ile 
225                 230                 235                 240 


Ile Phe Val Gly His Asp Trp Gly Ala Ala Leu Ala Phe His Tyr Ala 
                245                 250                 255     


Tyr Glu His Gln Asp Arg Ile Lys Ala Ile Val His Met Glu Ser Val 
            260                 265                 270         


Val Asp Val Ile Glu Ser Trp Asp Glu Trp Pro Asp Ile Glu Glu Asp 
        275                 280                 285             


Ile Ala Leu Ile Lys Ser Glu Glu Gly Glu Lys Met Val Leu Glu Asn 
    290                 295                 300                 


Asn Phe Phe Val Glu Thr Val Leu Pro Ser Lys Ile Met Arg Lys Leu 
305                 310                 315                 320 


Glu Pro Glu Glu Phe Ala Ala Tyr Leu Glu Pro Phe Lys Glu Lys Gly 
                325                 330                 335     


Glu Val Arg Arg Pro Thr Leu Ser Trp Pro Arg Glu Ile Pro Leu Val 
            340                 345                 350         


Lys Gly Gly Lys Pro Asp Val Val Gln Ile Val Arg Asn Tyr Asn Ala 
        355                 360                 365             


Tyr Leu Arg Ala Ser Asp Asp Leu Pro Lys Leu Phe Ile Glu Ser Asp 
    370                 375                 380                 


Pro Gly Phe Phe Ser Asn Ala Ile Val Glu Gly Ala Lys Lys Phe Pro 
385                 390                 395                 400 


Asn Thr Glu Phe Val Lys Val Lys Gly Leu His Phe Leu Gln Glu Asp 
                405                 410                 415     


Ala Pro Asp Glu Met Gly Lys Tyr Ile Lys Ser Phe Val Glu Arg Val 
            420                 425                 430         


Leu Lys Asn Glu Gln Leu Pro Glu Thr Gly Gly Ala Ala Leu Glu Ala 
        435                 440                 445             


Ser 
    


<210>  7
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLAG peptide

<400>  7

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  8
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nanobody Sensor

<400>  8

Met Gly His His His His His His Gly Thr Glu Asn Leu Tyr Phe Gln 
1               5                   10                  15      


Ser Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Thr 
            20                  25                  30          


Gly Asp Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Tyr Thr 
        35                  40                  45              


Pro Tyr Ala Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu 
    50                  55                  60                  


Phe Val Ala Gly Ile Gly Gly Ile Asp Gly Thr Ala Ala Tyr Ala Asp 
65                  70                  75                  80  


Ser Val Arg Gly Arg Ala Thr Ile Ser Arg Asp Ser Ala Lys Lys Thr 
                85                  90                  95      


Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr 
            100                 105                 110         


Ser Cys Ala Thr Arg Ala Ser Met Gln Val Leu Thr Ser Pro Arg Val 
        115                 120                 125             


Tyr Pro Ile Trp Gly Arg Gly Thr Gln Val Thr Val Ser Ser Gly Ser 
    130                 135                 140                 


Ala Ser Lys Val Tyr Asp Pro Glu Gln Arg Lys Arg Met Ile Thr Gly 
145                 150                 155                 160 


Pro Gln Trp Trp Ala Arg Cys Lys Gln Met Asn Val Leu Asp Ser Phe 
                165                 170                 175     


Ile Asn Tyr Tyr Asp Ser Glu Lys His Ala Glu Asn Ala Val Ile Phe 
            180                 185                 190         


Leu His Gly Asn Ala Thr Ser Ser Tyr Leu Trp Arg His Val Val Pro 
        195                 200                 205             


His Ile Glu Pro Val Ala Arg Cys Ile Ile Pro Asp Leu Ile Gly Met 
    210                 215                 220                 


Gly Lys Ser Gly Lys Ser Gly Asn Gly Ser Tyr Arg Leu Leu Asp His 
225                 230                 235                 240 


Tyr Lys Tyr Leu Thr Ala Trp Phe Glu Leu Leu Asn Leu Pro Lys Lys 
                245                 250                 255     


Ile Ile Phe Val Gly His Asp Trp Gly Ala Ala Leu Ala Phe His Tyr 
            260                 265                 270         


Ala Tyr Glu His Gln Asp Arg Ile Lys Ala Ile Val His Met Glu Ser 
        275                 280                 285             


Val Val Asp Val Ile Glu Ser Trp Asp Glu Trp Pro Asp Ile Glu Glu 
    290                 295                 300                 


Asp Ile Ala Leu Ile Lys Ser Glu Glu Gly Glu Lys Met Val Leu Glu 
305                 310                 315                 320 


Asn Asn Phe Phe Val Glu Thr Val Leu Pro Ser Lys Ile Met Arg Lys 
                325                 330                 335     


Leu Glu Pro Glu Glu Phe Ala Ala Tyr Leu Glu Pro Phe Lys Glu Lys 
            340                 345                 350         


Gly Glu Val Arg Arg Pro Thr Leu Ser Trp Pro Arg Glu Ile Pro Leu 
        355                 360                 365             


Val Lys Gly Gly Lys Pro Asp Val Val Gln Ile Val Arg Asn Tyr Asn 
    370                 375                 380                 


Ala Tyr Leu Arg Ala Ser Asp Asp Leu Pro Lys Leu Phe Ile Glu Ser 
385                 390                 395                 400 


Asp Pro Gly Phe Phe Ser Asn Ala Ile Val Glu Gly Ala Lys Lys Phe 
                405                 410                 415     


Pro Asn Thr Glu Phe Val Lys Val Lys Gly Leu His Phe Leu Gln Glu 
            420                 425                 430         


Asp Ala Pro Asp Glu Met Gly Lys Tyr Ile Lys Ser Phe Val Glu Arg 
        435                 440                 445             


Val Leu Lys Asn Glu Gln Leu Pro Glu Thr Gly Gly Ala Ala Leu Glu 
    450                 455                 460                 


Ala Ser 
465     


