SEQUENCE LISTING

<110> APPLICANT: University of Utah Research Foundation
<120> TITLE OF INVENTION: Metabolic engineering of non-pathogenic escherichia coli strains for the controlled production of low molecular weight heparosan and size-specific 
<160> NUMBER OF SEQ ID NOS.: 7
<210> SEQ ID NO 1
<211> LENGTH: 6517
<212> TYPE: DNA
<213> ORGANISM: E. coli
<223> OTHER INFORMATION: pBAD-myc/hisA-elmA-1 plasmid sequence
<400> SEQUENCE: 1

aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct	        60
tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca	        120
aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg	        180
attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg	        240
atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc	        300
taacaggagg aattaacc atg gcg gtc tca acc gaa gtt gac cac aac gaa tac aca ggt	360
aac ggc gtt acg aca tca ttt ccg tat acc ttc cgt att ttc aaa aaa tcc gac ctg gtt	420
gtt cag gtg tct gac ctt aac ggt aac gtt aca aaa cta gtg ctg gat gct ggt tat acg	480
gta aca ggg gcg gga act tat agt ggc ggt gca gtg gtt ctt ccg tcg ccg ctt gct gct	540
ggc tgg cga atc acg ata gag cgt gtg ctt gat gtg gtg cag gag act gat ctt cgc aat	600
cag gga aaa ttt ttc ccc gaa gtt cat gag gat gca ttt gac tac ctg acg atg ctg atc	660
cag cga tgt ttt ggg tgg ttc aga cgt gca ttg atg aaa cca tct ttg ctt gca aaa tat	720
tac gat gca aag caa aac aga ata tct aac ctt gcc gat cca tca ctt gag cag gac gct	780
gta aat aat cgc tca atg cgt aat tat gtc gat gct gca atc gcc gga gtt att ggt ggt	840
ttt ggt tgg ttt att cag tat ggt tct gga gcg gta tac aga acg ttc cag gat aag atg	900
cgt gat ggt gtc agc att aag gat ttt gga gct caa aat gga atc tta aat gat aac aag	960
gat gct ttt aca aaa tca tta cat tcg ttt agc agt gtt ttt gtt ccg gaa ggg gta ttc	1020
aat aca tct tta gtt tct ctt tca cgt tgt ggc ttg tac gga aca ggt ggg gga acg ata	1080
aaa cag tat gac aga gat ggt aat cat ctg gtt ttt aac atg ccc gat ggt ggc atg ctt	1140
agt acg cta aca att atg gga aat aaa tca gat gat agt gtg cag gga cac cag gtg tca	1200
ttt tca ggt ggc cat gat gta tcg gtt aaa aat atc aga ttt aca aat acg cga gga cca	1280
gga ttt agc ttg atc gct tat ccg gat aat ggt att ccg tca ggt tac att gtt aga gat	1340
ata aga gga gag tat tta ggg ttc gca aat aat aaa aaa gca ggt tgt gtg ctt ttt gat	1400
tca tcg caa aat acg cta att gat ggt gtg ata gcc aga aat tat cct cag ttt ggt gca	1460
gtg gaa ctt aaa aca gca gca aaa tat aac att gtc agc aat gtt att ggt gaa gag tgt	1500
cag cac gtt gtt tac aat gga act gag acg gaa act gcc cca acg aat aat atc att agc	1560
agt gta atg gct aac aac cca aaa tac gcc gca gta gtt gtt ggc aag ggg act ggt aac	1620
ctg att tcg gat gtg ctg gtt gat tac tct gaa tcg gac gca aag cag gcg cac ggc gtc	1700
acc gtt cag gga aat aat aat att gcc agt aat att cta atg act ggg tgt gat ggg aaa	1760
aat gaa tca gga gat ctg cag aca tct aca acc att cgt ttc tta gat gct gca cgc agt	1820
aat tat gcg tca ata ttc ccc atg tat agt tct tcc ggc gtg gtt acc ttc gag gaa ggg	1880
tgt atc agg aac ttt gtt gaa att aaa cat ccg ggt gac aga aat aat att ctg agt tct	1940
gca tca gcg gtg act ggt att tcc agt ata gac ggc act aca aat agc aat gtt gtt cac	2020
gtc cct gcg ctt ggt cag tac gtt ggg act atg tca ggg cgt ttt gaa tgg tgg gtt aaa	2080
tat ttt aac ctt gct aac cag acg ctt gtt tct gca gat aaa ttc aga atg ctt gct gaa	2140
ggc gat gta tct ctg gct gtg gga ggc ggt ata agt tcg caa ttg aaa tta ttc aat agt	2200
gat aat act aaa ggc act atg tcg cta ata aat gga aat att cga ata tct act gga aat	2260
tca gaa tat ata cag ttt tct gat tca gcc atg aca cca tcg aca acg aat act tat tct	2320
ctt ggg ttg gct ggt cgt gca tgg tcg ggg gga ttt acc cag tca gcg ttt acg gtg ctg	2380
tcc gat gcg cgt ttc aag act gct cca gag gtt att gat gag aaa ata ctg gac gca tgg	2440
gaa aga gtg gaa tgg gtt tca tac cag tac ctt gac agg atc gaa gtg aaa ggt aaa gac	2500
gga gca aga tgg cac ttt ggt gca gtt gcg cag cat gtt atc agt gta ttt cag aat gaa	2560
ggc ata gat gtg tca cga ctg gca ttt atc tgt tat gac aag tgg aat gag acc ccg gca	2600
gaa tac agg gat gtg acg gaa gaa gag cat tct gca gga gtt tac cca ctt ata cag aca	2660
aag gtt ctg gta cgc gaa gcc gtc gag gct ggt gaa tgt tac ggt atc cgt tat gaa gag	2720
gct ctg att ctg gaa tct gcg atg atg aga cgc agg gtt aaa aag ctg gaa gag caa gtt	2780
ttg caa tta aca ggg aat tggaattcga agcttgggcc cgaacaaaaa ctcatctcag 	        2838
aagaggatct gaatagcgcc gtcgaccatc atcatcatca tcattgagtt taaacggtct	        2898
ccagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga	        2958
acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc	        3018
tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc	        3078
ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact	        3138
gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc	        3258
cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc	        3318
cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt	        3378
ttctacaaac tcttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga	        3438
caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgagt attcaacatt	        3498
tccgtgtcgc ccttattccc ttttttgcggc attttgcctt cctgtttttgc tcacccagaa	        3558
acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa	        3618
ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg	        3678
atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa	        3738
gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc	        3798
acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc	        3858
atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta	        3918
accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag	        3978
ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca	        4038
acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata	        4098
gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc	        4158
tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca	        4218
ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca	        4278
actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg	        4338
taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa	        4398
tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt	        4458
gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat	        4518
cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg	        4578
gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga	        4638
gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac	        4698
tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt	        4758
ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag	        4818
cggtcgggctg aacggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga	        4878
actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc	        4938
ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg	        4998
gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg	        5058
atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt	        5118
tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc	        5178
tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg	        5238
aacgaccgag cgcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt	        5898
ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc	        5958
tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg	        6018
ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg	        6078
catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac	        6138
cgtcatcacc gaaacgcgcg aggcagcaga tcaattcgcg cgcgaaggcg aagcggcatg	        6198
cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca	        6258
agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt	        6318
tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc	        6378
gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc	        6438
gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga	        6498
cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat	        6558
gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac	        6618
aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc	        6678
gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc	        6738
cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat	        6798
ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt	        6858
aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac	        6918
cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt	        6978
ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt	        7038
tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta	        7098
aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt	        7158
cagccatact tttcatactc ccgccattca gag	                                        7191


<110> APPLICANT: University of Utah Research Foundation
<120> TITLE OF INVENTION: Metabolic engineering of non-pathogenic escherichia coli strains for the controlled production of low molecular weight heparosan and size-specific 
<160> NUMBER OF SEQ ID NOS.: 7
<210> SEQ ID NO 2
<211> LENGTH: 6184
<212> TYPE: DNA
<213> ORGANISM: E. coli
<223> OTHER INFORMATION: pBAD-myc/hisA-elmA-2 plasmid sequence
<400> SEQUENCE: 2

aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct	        60
tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca	        120
aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg	        180
attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg	        240
atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc	        300
taacaggagg aattaacc atg gtg atc cag cga tgt ttt ggg tgg ttc aga cgt gca	        359
ttg atg aaa cca tct ttg ctt gca aaa tat tac gat gca aag caa aac aga ata tct aac	419
ctt gcc gat cca tca ctt gag cag gac gct gta aat aat cgc tca atg cgt aat tat gtc	479
gat gct gca atc gcc gga gtt att ggt ggt ttt ggt tgg ttt att cag tat ggt tct gga	539
gcg gta tac aga acg ttc cag gat aag atg cgt gat ggt gtc agc att aag gat ttt gga	599
gct caa aat gga atc tta aat gat aac aag gat gct ttt aca aaa tca tta cat tcg ttt	659
agc agt gtt ttt gtt ccg gaa ggg gta ttc aat aca tct tta gtt tct ctt tca cgt tgt	719
ggc ttg tac gga aca ggt ggg gga acg ata aaa cag tat gac aga gat ggt aat cat ctg	779
gtt ttt aac atg ccc gat ggt ggc atg ctt agt acg cta aca att atg gga aat aaa tca	839
gat gat agt gtg cag gga cac cag gtg tca ttt tca ggt ggc cat gat gta tcg gtt aaa	899
aat atc aga ttt aca aat acg cga gga cca gga ttt agc ttg atc gct tat ccg gat aat	959
ggt att ccg tca ggt tac att gtt aga gat ata aga gga gag tat tta ggg ttc gca aat	1019
aat aaa aaa gca ggt tgt gtg ctt ttt gat tca tcg caa aat acg cta att gat ggt gtg	1079
ata gcc aga aat tat cct cag ttt ggt gca gtg gaa ctt aaa aca gca gca aaa tat aac	1139
att gtc agc aat gtt att ggt gaa gag tgt cag cac gtt gtt tac aat gga act gag acg	1199
gaa act gcc cca acg aat aat atc att agc agt gta atg gct aac aac cca aaa tac gcc	2458
gca gta gtt gtt ggc aag ggg act ggt aac ctg att tcg gat gtg ctg gtt gat tac tct	2518
gaa tcg gac gca aag cag gcg cac ggc gtc acc gtt cag gga aat aat aat att gcc agt	2578
aat att cta atg act ggg tgt gat ggg aaa aat gaa tca gga gat ctg cag aca tct aca	2638
acc att cgt ttc tta gat gct gca cgc agt aat tat gcg tca ata ttc ccc atg tat agt	2698
tct tcc ggc gtg gtt acc ttc gag gaa ggg tgt atc agg aac ttt gtt gaa att aaa cat	2758
ccg ggt gac aga aat aat att ctg agt tct gca tca gcg gtg act ggt att tcc agt ata	2818
gac ggc act aca aat agc aat gtt gtt cac gtc cct gcg ctt ggt cag tac gtt ggg act	2878
atg tca ggg cgt ttt gaa tgg tgg gtt aaa tat ttt aac ctt gct aac cag acg ctt gtt	2938
tct gca gat aaa ttc aga atg ctt gct gaa ggc gat gta tct ctg gct gtg gga ggc ggt	2998
ata agt tcg caa ttg aaa tta ttc aat agt gat aat act aaa ggc act atg tcg cta ata	3058
aat gga aat att cga ata tct act gga aat tca gaa tat ata cag ttt tct gat tca gcc	3118
atg aca cca tcg aca acg aat act tat tct ctt ggg ttg gct ggt cgt gca tgg tcg ggg	3178
gga ttt acc cag tca gcg ttt acg gtg ctg tcc gat gcg cgt ttc aag act gct cca gag	3238
gtt att gat gag aaa ata ctg gac gca tgg gaa aga gtg gaa tgg gtt tca tac cag tac	3298
ctt gac agg atc gaa gtg aaa ggt aaa gac gga gca aga tgg cac ttt ggt gca gtt gcg	3358
cag cat gtt atc agt gta ttt cag aat gaa ggc ata gat gtg tca cga ctg gca ttt atc	3418
tgt tat gac aag tgg aat gag acc ccg gca gaa tac agg gat gtg acg gaa gaa gag cat	3478
tct gca gga gtt tac cca ctt ata cag aca aag gtt ctg gta cgc gaa gcc gtc gag gct	3538
ggt gaa tgt tac ggt atc cgt tat gaa gag gct ctg att ctg gaa tct gcg atg atg aga	3598
cgc agg gtt aaa aag ctg gaa gag caa gtt ttg caa tta aca ggg aa ttggaattcg	3655
aagcttgggc ccgaacaaaa actcatctca gaagaggatc tgaatagcgc cgtcgaccat	        3715
catcatcatc atcattgagt ttaaacggtc tccagcttgg ctgttttggc ggatgagaga	        3775
agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt	        3835
tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac	        3895
gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat	        3955
caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg	        4015
gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa	        4075
cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag	        4135
aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta	        4195
aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata	        4255
ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc	        4375
agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct	        4425
tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg	        4495
tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta	        4555
ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat	        4515
gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt	        4675
acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga	        4735
tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga	        4795
gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga	        4855
actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc	        4915
aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc	        4975
cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg	        5035
tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat	        5095
cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata	        5155
tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct	        5215
ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga	        5275
ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg	        5335
cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc	        5395
aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct	        5455
agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc	        5515
tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt	        5575
ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg	        5635
cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct	        5695
atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag	        5755
ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag	        5815
tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg	        5875
gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg	        5935
gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac	        5995
cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt	        6055
gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat	        6115
ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca	        6175
gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca	        6235
cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg	        6295
accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg	        6355
cagcagatca attcgcgcgc gaaggcgaag cggcatgcat aatgtgcctg tcaaatggac	        6415
gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt	        6475
accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac	        6535
tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca	        6595
aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc	        6655
gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg	        6775
aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca	        6835
aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc	        6895
atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc	        6955
agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc	        7015
ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg	        7075
gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc	        7135
cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc	        7195
gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc	        7255
ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata	        7315
aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta	        7435
aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg	        7495
ccattcagag	7505

<110> APPLICANT: University of Utah Research Foundation
<120> TITLE OF INVENTION: Metabolic engineering of non-pathogenic escherichia coli strains for the controlled production of low molecular weight heparosan and size-specific 
<160> NUMBER OF SEQ ID NOS.: 6
<210> SEQ ID NO 3
<211> LENGTH: 5938
<212> TYPE: DNA
<213> ORGANISM: E. coli
<223> OTHER INFORMATION: pBAD-myc/hisA-elmA-3 plasmid sequence
<400> SEQUENCE: 3

aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct	        60
tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca	        120
aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg	        180
attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg	        240
atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc	        300
taacaggagg aattaacc atg ggt gat ggt gtc agc att aag gat ttt gga gct caa	        359
aat gga atc tta aat gat aac aag gat gct ttt aca aaa tca tta cat tcg ttt agc agt	419
gtt ttt gtt ccg gaa ggg gta ttc aat aca tct tta gtt tct ctt tca cgt tgt ggc ttg	479
tac gga aca ggt ggg gga acg ata aaa cag tat gac aga gat ggt aat cat ctg gtt	539
ttt aac atg ccc gat ggt ggc atg ctt agt acg cta aca att atg gga aat aaa tca gat	599
gat agt gtg cag gga cac cag gtg tca ttt tca ggt ggc cat gat gta tcg gtt aaa	659
aat atc aga ttt aca aat acg cga gga cca gga ttt agc ttg atc gct tat ccg gat aat	719
ggt att ccg tca ggt tac att gtt aga gat ata aga gga gag tat tta ggg ttc gca	779
aat aat aaa aaa gca ggt tgt gtg ctt ttt gat tca tcg caa aat acg cta att gat ggt	839
gtg ata gcc aga aat tat cct cag ttt ggt gca gtg gaa ctt aaa aca gca gca aaa	899
tat aac att gtc agc aat gtt att ggt gaa gag tgt cag cac gtt gtt tac aat gga act	959
gag acg gaa act gcc cca acg aat aat atc att agc agt gta atg gct aac aac cca	1019
aaa tac gcc gca gta gtt gtt ggc aag ggg act ggt aac ctg att tcg gat gtg ctg gtt	1079
gat tac tct gaa tcg gac gca aag cag gcg cac ggc gtc acc gtt cag gga aat aat	1139
aat att gcc agt aat att cta atg act ggg tgt gat ggg aaa aat gaa tca gga gat ctg	1199
cag aca tct aca acc att cgt ttc tta gat gct gca cgc agt aat tat gcg tca ata	1259
ttc ccc atg tat agt tct tcc ggc gtg gtt acc ttc gag gaa ggg tgt atc agg aac ttt	1319
gtt gaa att aaa cat ccg ggt gac aga aat aat att ctg agt tct gca tca gcg gtg	1379
act ggt att tcc agt ata gac ggc act aca aat agc aat gtt gtt cac gtc cct gcg ctt	1439
ggt1499 cag tac gtt ggg act atg tca ggg cgt ttt gaa tgg tgg gtt aaa tat ttt aac	1499
ctt gct aac cag acg ctt gtt tct gca gat aaa ttc aga atg ctt gct gaa ggc gat gta	1559
tct ctg gct gtg gga ggc ggt ata agt tcg caa ttg aaa tta ttc aat agt gat aat	1619
act aaa ggc act atg tcg cta ata aat gga aat att cga ata tct act gga aat tca gaa	1679
tat ata cag ttt tct gat tca gcc atg aca cca tcg aca acg aat act tat tct ctt	1739
ggg ttg gct ggt cgt gca tgg tcg ggg gga ttt acc cag tca gcg ttt acg gtg ctg tcc	1799
gat gcg cgt ttc aag act gct cca gag gtt att gat gag aaa ata ctg gac gca tgg	1859
gaa aga gtg gaa tgg gtt tca tac cag tac ctt gac agg atc gaa gtg aaa ggt aaa gac	1919
gga gca aga tgg cac ttt ggt gca gtt gcg cag cat gtt atc agt gta ttt cag aat	1979
gaa ggc ata gat gtg tca cga ctg gca ttt atc tgt tat gac aag tgg aat gag acc ccg	2039
gca gaa tac agg gat gtg acg gaa gaa gag cat tct gca gga gtt tac cca ctt ata	2099
cag aca aag gtt ctg gta cgc gaa gcc gtc gag gct ggt gaa tgt tac ggt atc cgt tat	2159
gaa gag gct ctg att ctg gaa tct gcg atg atg aga cgc agg gtt aaa aag ctg gaa	2219
gag caa gtt ttg caa tta aca ggg aa ttggaattcg aagcttgggc ccgaacaaaa	        2275
actcatctca gaagaggatc tgaatagcgc cgtcgaccat catcatcatc atcattgagt	        2335
ttaaacggtc tccagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag	        2455
attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg	        2515
gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt	        2575
gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca	        2635
gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag	        2695
gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc	        2755
aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg	        2875
cctttttgcg tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc	        2935
cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga	        3055
gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt	        3115
ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag	        3175
tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag	        3235
aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg	        3295
ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg	        3355
agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca	        3415
gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag	        3475
gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc	        3535
gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg	        3595
tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc	        3655
ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg	        3715
cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg	        3775
gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga	        3835
cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac	        3895
tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa	        3955
aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca	        4015
aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag	        4075
gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac	        4135
cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa	        4195
ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc	        4255
accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag	        4315
tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac	        4375
cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc	        4435
gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc	        4495
ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca	        4555
cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc	        4615
tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg	        4675
ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct	        4735
ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata	        4795
ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc	        4855
gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca	        4915
ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct	        4975
acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg	        5035
ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat	        5095
gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagcagatca attcgcgcgc	        5155
gaaggcgaag cggcatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac	        5215
cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac	        5275
ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt	        5335
gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac	        5395
ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc	        5455
ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga	        5515
cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg	        5575
atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc	        5635
gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc	        5695
cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg	        5755
cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt	        5815
aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg	        5875
agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc	        5935
cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag	        5995
attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt	        6055
ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag	        6115
gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag	                        6165

<110> APPLICANT: University of Utah Research Foundation
<120> TITLE OF INVENTION: Metabolic engineering of non-pathogenic escherichia coli strains for the controlled production of low molecular weight heparosan and size-specific 
<160> NUMBER OF SEQ ID NOS.: 7
<210> SEQ ID NO 4
<211> LENGTH: 24
<212> TYPE: DNA
<213> ORGANISM: Artificial sequence
<220> FEATURE: primer_bind
<223> OTHER INFORMATION: Forward primer for SEQ ID NO 1
<400> SEQUENCE: 4

aagtccatgg cggtctcaac cgaa                            24

<110> APPLICANT: University of Utah Research Foundation
<120> TITLE OF INVENTION: Metabolic engineering of non-pathogenic escherichia coli strains for the controlled production of low molecular weight heparosan and size-specific 
<160> NUMBER OF SEQ ID NOS.: 7
<210> SEQ ID NO 5
<211> LENGTH: 30
<212> TYPE: DNA
<213> ORGANISM: Artificial sequence
<220> FEATURE: primer_bind
<223> OTHER INFORMATION: Forward Primer for SEQ ID NO 2
<400> SEQUENCE: 5

aagtccatgg tgatccagcg atgttttggg                      30

<110> APPLICANT: University of Utah Research Foundation
<120> TITLE OF INVENTION: Metabolic engineering of non-pathogenic escherichia coli strains for the controlled production of low molecular weight heparosan and size-specific heparosan oligosaccharides
<130> FILE REFERENCE: 76926-318144
<140> CURRENT PATENT APPLICATION: PCT/US2020/029902
<141> CURRENT FILING DATE: April 24, 2020
<150> EARLIER PATENT APPLICATIONS: U.S. Provisional Application Nos. 62/914,112 and 62/838,432
<151> EARLIER FILING DATES: Respectively, October 11, 2019 and April 25, 2019
<160> NUMBER OF SEQ ID NOS.: 7
<210> SEQ ID NO 6
<211> LENGTH: 31
<212> TYPE: DNA
<213> ORGANISM: Artificial sequence
<220> FEATURE: primer_bind
<223> OTHER INFORMATION: Forward Primer for SEQ ID NO 3
<400> SEQUENCE: 6

aagtccatgg gtgatggtgt cagcattaag g                   31
	
<110> APPLICANT: University of Utah Research Foundation
<120> TITLE OF INVENTION: Metabolic engineering of non-pathogenic escherichia coli strains for the controlled production of low molecular weight heparosan and size-specific heparosan OLIGOSACCHARIDES
<160> NUMBER OF SEQ ID NOS.: 7
<210> SEQ ID NO: 7
<211> LENGTH: 32
<212> TYPE: DNA
<213> ORGANISM: Artificial sequence
<220> FEATURE: primer_bind
<223> OTHER INFORMATION: Reverse Primer for SEQ ID NOs 1, 2, and 3
<400> SEQUENCE: 7

aaggaattca attccctgtt aattgcaaaa ct                   32

