                         SEQUENCE LISTING

<110>  The Trustees of the University of Pennsylvania
 
<120>  COMPOSITIONS USEFUL IN TREATMENT OF RETT SYNDROME

<130>  UPN-19-9007PCT

<150>  US 62/837,947
<151>  2019-04-24

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  5986
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Production plasmid 1


<220>
<221>  repeat_region
<222>  (1)..(130)
<223>  ITR

<220>
<221>  promoter
<222>  (213)..(678)
<223>  hSynapsin

<220>
<221>  misc_feature
<222>  (690)..(695)
<223>  Kozak

<220>
<221>  CDS
<222>  (696)..(2195)
<223>  human MeCP2alpha

<220>
<221>  misc_feature
<222>  (2190)..(2195)
<223>  Stop codons

<220>
<221>  polyA_signal
<222>  (2303)..(2534)
<223>  SV40 late polyadenylation

<220>
<221>  repeat_region
<222>  (2599)..(2728)
<223>  ITR

<220>
<221>  misc_feature
<222>  (2905)..(3343)
<223>  f1 ori

<220>
<221>  misc_feature
<222>  (3372)..(4014)
<223>  pUC origin of replication

<220>
<221>  misc_feature
<222>  (4689)..(5504)
<223>  Kan-r

<220>
<221>  misc_feature
<222>  (5747)..(5932)
<223>  Lac promoter

<400>  1
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct      180

aggaagatcc tctagaacta tagctagcat gcctgcagag ggccctgcgt atgagtgcaa      240

gtgggtttta ggaccaggat gaggcggggt gggggtgcct acctgacgac cgaccccgac      300

ccactggaca agcacccaac ccccattccc caaattgcgc atcccctatc agagaggggg      360

aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc gcggacagtg      420

ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg      480

tcactcgccg gtcccccgca aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc      540

gccggcccag ccggaccgca ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat      600

ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc      660

gtgtcgtgcc tgagagcgca gtcgaattcg ccacc atg gct gct gca gct gct         713
                                       Met Ala Ala Ala Ala Ala            
                                       1               5                  

gct gca cct tct ggt ggt ggc gga ggc gga gag gaa gag aga ctg gaa        761
Ala Ala Pro Ser Gly Gly Gly Gly Gly Gly Glu Glu Glu Arg Leu Glu           
            10                  15                  20                    

gag aag tcc gag gac cag gat ctg cag ggc ctg aag gac aag ccc ctg        809
Glu Lys Ser Glu Asp Gln Asp Leu Gln Gly Leu Lys Asp Lys Pro Leu           
        25                  30                  35                        

aag ttc aag aaa gtg aag aag gat aag aaa gag gaa aaa gag ggc aag        857
Lys Phe Lys Lys Val Lys Lys Asp Lys Lys Glu Glu Lys Glu Gly Lys           
    40                  45                  50                            

cac gag ccc gtg cag cca tct gct cac cat tct gcc gaa cct gcc gaa        905
His Glu Pro Val Gln Pro Ser Ala His His Ser Ala Glu Pro Ala Glu           
55                  60                  65                  70            

gcc gga aag gcc gaa aca tct gaa ggc tct gga agc gcc cct gcc gtg        953
Ala Gly Lys Ala Glu Thr Ser Glu Gly Ser Gly Ser Ala Pro Ala Val           
                75                  80                  85                

cct gaa gct tct gct tct cct aag cag cgg cgg agc atc atc aga gac       1001
Pro Glu Ala Ser Ala Ser Pro Lys Gln Arg Arg Ser Ile Ile Arg Asp           
            90                  95                  100                   

aga ggc cct atg tac gac gac ccc aca ctg cct gaa ggc tgg acc aga       1049
Arg Gly Pro Met Tyr Asp Asp Pro Thr Leu Pro Glu Gly Trp Thr Arg           
        105                 110                 115                       

aag ctg aag cag aga aag agc ggc aga agc gcc ggg aag tac gac gtg       1097
Lys Leu Lys Gln Arg Lys Ser Gly Arg Ser Ala Gly Lys Tyr Asp Val           
    120                 125                 130                           

tac ctg atc aac cct cag ggc aaa gcc ttc cgg tcc aag gtg gaa ctg       1145
Tyr Leu Ile Asn Pro Gln Gly Lys Ala Phe Arg Ser Lys Val Glu Leu           
135                 140                 145                 150           

atc gcc tac ttc gag aaa gtg ggc gac acc tct ctg gac ccc aac gac       1193
Ile Ala Tyr Phe Glu Lys Val Gly Asp Thr Ser Leu Asp Pro Asn Asp           
                155                 160                 165               

ttc gat ttc acc gtg aca ggc aga ggc agc ccc agc aga aga gag cag       1241
Phe Asp Phe Thr Val Thr Gly Arg Gly Ser Pro Ser Arg Arg Glu Gln           
            170                 175                 180                   

aag cct cct aag aag cct aag agc ccc aag gct cct ggc acc gga aga       1289
Lys Pro Pro Lys Lys Pro Lys Ser Pro Lys Ala Pro Gly Thr Gly Arg           
        185                 190                 195                       

ggt aga ggt aga cct aaa ggc agc ggc acc aca aga cct aag gcc gct       1337
Gly Arg Gly Arg Pro Lys Gly Ser Gly Thr Thr Arg Pro Lys Ala Ala           
    200                 205                 210                           

act tct gag ggc gtg caa gtg aag aga gtg ctg gaa aag agc ccc ggc       1385
Thr Ser Glu Gly Val Gln Val Lys Arg Val Leu Glu Lys Ser Pro Gly           
215                 220                 225                 230           

aag ctg ctg gtc aag atg ccc ttt cag aca agc cct ggc ggc aaa gca       1433
Lys Leu Leu Val Lys Met Pro Phe Gln Thr Ser Pro Gly Gly Lys Ala           
                235                 240                 245               

gaa ggc gga ggg gct aca aca agc acc caa gtg atg gtc atc aag agg       1481
Glu Gly Gly Gly Ala Thr Thr Ser Thr Gln Val Met Val Ile Lys Arg           
            250                 255                 260                   

ccc ggc aga aag cgg aag gcc gaa gct gat cct cag gct atc ccc aag       1529
Pro Gly Arg Lys Arg Lys Ala Glu Ala Asp Pro Gln Ala Ile Pro Lys           
        265                 270                 275                       

aag aga ggc cgg aag cct gga tct gtg gtt gct gct gcc gcc gca gag       1577
Lys Arg Gly Arg Lys Pro Gly Ser Val Val Ala Ala Ala Ala Ala Glu           
    280                 285                 290                           

gcc aag aaa aag gcc gtg aaa gag tcc agc atc cgc agc gtg caa gag       1625
Ala Lys Lys Lys Ala Val Lys Glu Ser Ser Ile Arg Ser Val Gln Glu           
295                 300                 305                 310           

aca gtg ctg ccc atc aag aag cgc aag acc cgg gaa acc gtg tcc atc       1673
Thr Val Leu Pro Ile Lys Lys Arg Lys Thr Arg Glu Thr Val Ser Ile           
                315                 320                 325               

gaa gtg aaa gag gtg gtc aag cct ctg ctg gtg tct acc ctg ggc gag       1721
Glu Val Lys Glu Val Val Lys Pro Leu Leu Val Ser Thr Leu Gly Glu           
            330                 335                 340                   

aag tct ggc aag gga ctg aaa acc tgc aag tcc cct ggc aga aag agc       1769
Lys Ser Gly Lys Gly Leu Lys Thr Cys Lys Ser Pro Gly Arg Lys Ser           
        345                 350                 355                       

aaa gag tct agc cct aag ggc aga agc agc agc gct agc tcc cca cct       1817
Lys Glu Ser Ser Pro Lys Gly Arg Ser Ser Ser Ala Ser Ser Pro Pro           
    360                 365                 370                           

aag aaa gaa cac cac cat cac cac cac cat agc gag tcc cca aag gct       1865
Lys Lys Glu His His His His His His His Ser Glu Ser Pro Lys Ala           
375                 380                 385                 390           

cca gtt cct ctg ctt cct cca ctg cct cca cct cca cct gag cct gag       1913
Pro Val Pro Leu Leu Pro Pro Leu Pro Pro Pro Pro Pro Glu Pro Glu           
                395                 400                 405               

tct agc gag gat cct aca agc cct cct gag cca cag gat ctg agc agc       1961
Ser Ser Glu Asp Pro Thr Ser Pro Pro Glu Pro Gln Asp Leu Ser Ser           
            410                 415                 420                   

tcc gtg tgc aaa gaa gag aag atg ccc aga ggc ggc tcc ctg gaa tct       2009
Ser Val Cys Lys Glu Glu Lys Met Pro Arg Gly Gly Ser Leu Glu Ser           
        425                 430                 435                       

gac ggc tgt cct aaa gag ccc gcc aag aca cag cct gcc gtt gcc aca       2057
Asp Gly Cys Pro Lys Glu Pro Ala Lys Thr Gln Pro Ala Val Ala Thr           
    440                 445                 450                           

gct gca aca gct gcc gag aag tac aag cac aga ggc gag ggc gag cgc       2105
Ala Ala Thr Ala Ala Glu Lys Tyr Lys His Arg Gly Glu Gly Glu Arg           
455                 460                 465                 470           

aag gat atc gtg tct agc agc atg ccc aga cct aac cgc gag gaa ccc       2153
Lys Asp Ile Val Ser Ser Ser Met Pro Arg Pro Asn Arg Glu Glu Pro           
                475                 480                 485               

gtg gat agc aga acc cct gtg acc gag agg gtg tcc tga tga               2195
Val Asp Ser Arg Thr Pro Val Thr Glu Arg Val Ser                           
            490                 495                                       

ctcgacggcg cgcccatggc gatatccaat tggagctcgc ggccgcctcg acttaccggt     2255

ttgatatctt ctagaaggat ccaacgcgta ggtaccggcg gccgcttcga gcagacatga     2315

taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta     2375

tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag     2435

ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt     2495

tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatctt cctagagcat     2555

ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg     2615

gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc     2675

gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagccttaat     2735

taacctaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc     2795

caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc     2855

cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt     2915

agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc     2975

agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc     3035

tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg     3095

cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga     3155

tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc     3215

caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg     3275

ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt     3335

aacaaaatca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg     3395

ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt     3455

cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc     3515

ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct     3575

tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc     3635

gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta     3695

tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca     3755

gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag     3815

tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag     3875

ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt     3935

agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa     3995

gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg     4055

attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttgat cctccggcgt     4115

tcagcctgtg ccacagccga caggatggtg accaccattt gccccatatc accgtcggta     4175

ctgatcccgt cgtcaataaa ccgaaccgct acaccctgag catcaaactc ttttatcagt     4235

tggatcatgt cggcggtgtc gcggccaaga cggtcgagct tcttcaccag aatgacatca     4295

ccttcctcca ccttcatcct cagcaaatcc agcccttccc gatctgttga actgccggat     4355

gccttgtcgg taaagatgcg gttagctttt acccctgcat ctttgagcgc tgaggtctgc     4415

ctcgtgaaga aggtgttgct gactcatacc aggcctgaat cgccccatca tccagccaga     4475

aagtgaggga gccacggttg atgagagctt tgttgtaggt ggaccagttg gtgattttga     4535

acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca     4595

actcagcaaa agttcgattt attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct     4655

ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg     4715

aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg     4775

taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc     4835

tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag     4895

gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt     4955

atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact     5015

cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc     5075

gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag     5135

cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt     5195

cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat     5255

ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc     5315

attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata     5375

caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata     5435

taaatcagca tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat     5495

atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga     5555

tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacca tgttctttcc     5615

tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc     5675

tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc     5735

aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag     5795

gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca     5855

ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag     5915

cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccagat ttaattaagg     5975

ccttaattag g                                                          5986


<210>  2
<211>  498
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  2

Met Ala Ala Ala Ala Ala Ala Ala Pro Ser Gly Gly Gly Gly Gly Gly 
1               5                   10                  15      


Glu Glu Glu Arg Leu Glu Glu Lys Ser Glu Asp Gln Asp Leu Gln Gly 
            20                  25                  30          


Leu Lys Asp Lys Pro Leu Lys Phe Lys Lys Val Lys Lys Asp Lys Lys 
        35                  40                  45              


Glu Glu Lys Glu Gly Lys His Glu Pro Val Gln Pro Ser Ala His His 
    50                  55                  60                  


Ser Ala Glu Pro Ala Glu Ala Gly Lys Ala Glu Thr Ser Glu Gly Ser 
65                  70                  75                  80  


Gly Ser Ala Pro Ala Val Pro Glu Ala Ser Ala Ser Pro Lys Gln Arg 
                85                  90                  95      


Arg Ser Ile Ile Arg Asp Arg Gly Pro Met Tyr Asp Asp Pro Thr Leu 
            100                 105                 110         


Pro Glu Gly Trp Thr Arg Lys Leu Lys Gln Arg Lys Ser Gly Arg Ser 
        115                 120                 125             


Ala Gly Lys Tyr Asp Val Tyr Leu Ile Asn Pro Gln Gly Lys Ala Phe 
    130                 135                 140                 


Arg Ser Lys Val Glu Leu Ile Ala Tyr Phe Glu Lys Val Gly Asp Thr 
145                 150                 155                 160 


Ser Leu Asp Pro Asn Asp Phe Asp Phe Thr Val Thr Gly Arg Gly Ser 
                165                 170                 175     


Pro Ser Arg Arg Glu Gln Lys Pro Pro Lys Lys Pro Lys Ser Pro Lys 
            180                 185                 190         


Ala Pro Gly Thr Gly Arg Gly Arg Gly Arg Pro Lys Gly Ser Gly Thr 
        195                 200                 205             


Thr Arg Pro Lys Ala Ala Thr Ser Glu Gly Val Gln Val Lys Arg Val 
    210                 215                 220                 


Leu Glu Lys Ser Pro Gly Lys Leu Leu Val Lys Met Pro Phe Gln Thr 
225                 230                 235                 240 


Ser Pro Gly Gly Lys Ala Glu Gly Gly Gly Ala Thr Thr Ser Thr Gln 
                245                 250                 255     


Val Met Val Ile Lys Arg Pro Gly Arg Lys Arg Lys Ala Glu Ala Asp 
            260                 265                 270         


Pro Gln Ala Ile Pro Lys Lys Arg Gly Arg Lys Pro Gly Ser Val Val 
        275                 280                 285             


Ala Ala Ala Ala Ala Glu Ala Lys Lys Lys Ala Val Lys Glu Ser Ser 
    290                 295                 300                 


Ile Arg Ser Val Gln Glu Thr Val Leu Pro Ile Lys Lys Arg Lys Thr 
305                 310                 315                 320 


Arg Glu Thr Val Ser Ile Glu Val Lys Glu Val Val Lys Pro Leu Leu 
                325                 330                 335     


Val Ser Thr Leu Gly Glu Lys Ser Gly Lys Gly Leu Lys Thr Cys Lys 
            340                 345                 350         


Ser Pro Gly Arg Lys Ser Lys Glu Ser Ser Pro Lys Gly Arg Ser Ser 
        355                 360                 365             


Ser Ala Ser Ser Pro Pro Lys Lys Glu His His His His His His His 
    370                 375                 380                 


Ser Glu Ser Pro Lys Ala Pro Val Pro Leu Leu Pro Pro Leu Pro Pro 
385                 390                 395                 400 


Pro Pro Pro Glu Pro Glu Ser Ser Glu Asp Pro Thr Ser Pro Pro Glu 
                405                 410                 415     


Pro Gln Asp Leu Ser Ser Ser Val Cys Lys Glu Glu Lys Met Pro Arg 
            420                 425                 430         


Gly Gly Ser Leu Glu Ser Asp Gly Cys Pro Lys Glu Pro Ala Lys Thr 
        435                 440                 445             


Gln Pro Ala Val Ala Thr Ala Ala Thr Ala Ala Glu Lys Tyr Lys His 
    450                 455                 460                 


Arg Gly Glu Gly Glu Arg Lys Asp Ile Val Ser Ser Ser Met Pro Arg 
465                 470                 475                 480 


Pro Asn Arg Glu Glu Pro Val Asp Ser Arg Thr Pro Val Thr Glu Arg 
                485                 490                 495     


Val Ser 
        


<210>  3
<211>  1500
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human MeCP2alpha

<400>  3
atggctgctg cagctgctgc tgcaccttct ggtggtggcg gaggcggaga ggaagagaga       60

ctggaagaga agtccgagga ccaggatctg cagggcctga aggacaagcc cctgaagttc      120

aagaaagtga agaaggataa gaaagaggaa aaagagggca agcacgagcc cgtgcagcca      180

tctgctcacc attctgccga acctgccgaa gccggaaagg ccgaaacatc tgaaggctct      240

ggaagcgccc ctgccgtgcc tgaagcttct gcttctccta agcagcggcg gagcatcatc      300

agagacagag gccctatgta cgacgacccc acactgcctg aaggctggac cagaaagctg      360

aagcagagaa agagcggcag aagcgccggg aagtacgacg tgtacctgat caaccctcag      420

ggcaaagcct tccggtccaa ggtggaactg atcgcctact tcgagaaagt gggcgacacc      480

tctctggacc ccaacgactt cgatttcacc gtgacaggca gaggcagccc cagcagaaga      540

gagcagaagc ctcctaagaa gcctaagagc cccaaggctc ctggcaccgg aagaggtaga      600

ggtagaccta aaggcagcgg caccacaaga cctaaggccg ctacttctga gggcgtgcaa      660

gtgaagagag tgctggaaaa gagccccggc aagctgctgg tcaagatgcc ctttcagaca      720

agccctggcg gcaaagcaga aggcggaggg gctacaacaa gcacccaagt gatggtcatc      780

aagaggcccg gcagaaagcg gaaggccgaa gctgatcctc aggctatccc caagaagaga      840

ggccggaagc ctggatctgt ggttgctgct gccgccgcag aggccaagaa aaaggccgtg      900

aaagagtcca gcatccgcag cgtgcaagag acagtgctgc ccatcaagaa gcgcaagacc      960

cgggaaaccg tgtccatcga agtgaaagag gtggtcaagc ctctgctggt gtctaccctg     1020

ggcgagaagt ctggcaaggg actgaaaacc tgcaagtccc ctggcagaaa gagcaaagag     1080

tctagcccta agggcagaag cagcagcgct agctccccac ctaagaaaga acaccaccat     1140

caccaccacc atagcgagtc cccaaaggct ccagttcctc tgcttcctcc actgcctcca     1200

cctccacctg agcctgagtc tagcgaggat cctacaagcc ctcctgagcc acaggatctg     1260

agcagctccg tgtgcaaaga agagaagatg cccagaggcg gctccctgga atctgacggc     1320

tgtcctaaag agcccgccaa gacacagcct gccgttgcca cagctgcaac agctgccgag     1380

aagtacaagc acagaggcga gggcgagcgc aaggatatcg tgtctagcag catgcccaga     1440

cctaaccgcg aggaacccgt ggatagcaga acccctgtga ccgagagggt gtcctgatga     1500


<210>  4
<211>  6769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Production plasmid 2


<220>
<221>  repeat_region
<222>  (1)..(130)
<223>  5' ITR

<220>
<221>  promoter
<222>  (198)..(579)
<223>  CMV IE promoter

<220>
<221>  promoter
<222>  (582)..(863)
<223>  CB promoter

<220>
<221>  TATA_signal
<222>  (836)..(839)
<223>  TATA

<220>
<221>  Intron
<222>  (957)..(1929)
<223>  chicken beta-actin intron

<220>
<221>  misc_feature
<222>  (1993)..(3486)
<223>  MECP2 e1

<220>
<221>  misc_feature
<222>  (3490)..(3537)
<223>  SpA

<220>
<221>  polyA_signal
<222>  (3604)..(3730)
<223>  Rabbit globin poly A

<220>
<221>  repeat_region
<222>  (3819)..(3948)
<223>  3' ITR

<220>
<221>  rep_origin
<222>  (4125)..(4580)
<223>  fi ori

<220>
<221>  misc_feature
<222>  (4711)..(5568)
<223>  AP(R)

<220>
<221>  rep_origin
<222>  (5742)..(6330)
<223>  Origin of replication

<400>  4
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg      180

atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat      240

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      300

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      360

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      420

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt      480

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc      540

tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac      600

gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat      660

tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg      720

ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca      780

gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa      840

aaagcgaagc gcgcggcggg cggggagtcg ctgcgcgctg ccttcgcccc gtgccccgct      900

ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga      960

gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg     1020

tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg     1080

ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg     1140

cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg     1200

tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga     1260

acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg     1320

tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg     1380

gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc     1440

aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc     1500

gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt     1560

tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga     1620

aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg     1680

gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc     1740

ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg     1800

cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg     1860

ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt     1920

ttggcaaaga attcctgtac ggaagtgtta cttctgctct aaaagctgcg gaattgtacc     1980

cgcgggccca ccatggccgc cgccgccgcc gccgcgccga gcggaggagg aggaggaggc     2040

gaggaggaga gactggaaga aaagtcagaa gaccaggacc tccagggcct caaggacaaa     2100

cccctcaagt ttaaaaaggt gaagaaagat aagaaagaag agaaagaggg caagcatgag     2160

cccgtgcagc catcagccca ccactctgct gagcccgcag aggcaggcaa agcagagaca     2220

tcagaagggt caggctccgc cccggctgtg ccggaagctt ctgcctcccc caaacagcgg     2280

cgctccatca tccgtgaccg gggacccatg tatgatgacc ccaccctgcc tgaaggctgg     2340

acacggaagc ttaagcaaag gaaatctggc cgctctgctg ggaagtatga tgtgtatttg     2400

atcaatcccc agggaaaagc ctttcgctct aaagtggagt tgattgcgta cttcgaaaag     2460

gtaggcgaca catccctgga ccctaatgat tttgacttca cggtaactgg gagagggagc     2520

ccctcccggc gagagcagaa accacctaag aagcccaaat ctcccaaagc tccaggaact     2580

ggcagaggcc ggggacgccc caaagggagc ggcaccacga gacccaaggc ggccacgtca     2640

gagggtgtgc aggtgaaaag ggtcctggag aaaagtcctg ggaagctcct tgtcaagatg     2700

ccttttcaaa cttcgccagg gggcaaggct gaggggggtg gggccaccac atccacccag     2760

gtcatggtga tcaaacgccc cggcaggaag cgaaaagctg aggccgaccc tcaggccatt     2820

cccaagaaac ggggccgaaa gccggggagt gtggtggcag ccgctgccgc cgaggccaaa     2880

aagaaagccg tgaaggagtc ttctatccga tctgtgcagg agaccgtact ccccatcaag     2940

aagcgcaaga cccgggagac ggtcagcatc gaggtcaagg aagtggtgaa gcccctgctg     3000

gtgtccaccc tcggtgagaa gagcgggaaa ggactgaaga cctgtaagag ccctgggcgg     3060

aaaagcaagg agagcagccc caaggggcgc agcagcagcg cctcctcacc ccccaagaag     3120

gagcaccacc accatcacca ccactcagag tccccaaagg cccccgtgcc actgctccca     3180

cccctgcccc cacctccacc tgagcccgag agctccgagg accccaccag cccccctgag     3240

ccccaggact tgagcagcag cgtctgcaaa gaggagaaga tgcccagagg aggctcactg     3300

gagagcgacg gctgccccaa ggagccagct aagactcagc ccgcggttgc caccgccgcc     3360

acggccgcag aaaagtacaa acaccgaggg gagggagagc gcaaagacat tgtttcatcc     3420

tccatgccaa ggccaaacag agaggagcct gtggacagcc ggacgcccgt gaccgagaga     3480

gttagctaga ataaagagct cagatgcatc gatcagagtg tgttggtttt ttgtgtgacg     3540

cgtggtacct ctagagtcga cccgggcggc ctcgaggacg gggtgaacta cgcctgagga     3600

tccgatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg     3660

acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt     3720

ctctcactcg gaagcaattc gttgatctga atttcgacca cccataatac ccattaccct     3780

ggtagataag tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt     3840

ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg     3900

acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagcc ttaattaacc     3960

taattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact     4020

taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac     4080

cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg     4140

cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc     4200

cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc     4260

ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct     4320

cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac     4380

ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac     4440

tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat     4500

ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa     4560

aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt     4620

tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa     4680

atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt     4740

attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa     4800

gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac     4860

agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt     4920

aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt     4980

cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat     5040

cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac     5100

actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg     5160

cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc     5220

ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa     5280

ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag     5340

gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct     5400

gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat     5460

ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa     5520

cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac     5580

caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc     5640

taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc     5700

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg     5760

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg     5820

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca     5880

aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg     5940

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg     6000

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga     6060

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac     6120

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat     6180

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc     6240

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga     6300

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc     6360

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg     6420

gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag     6480

cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc     6540

gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc     6600

agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac     6660

tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga     6720

aacagctatg accatgatta cgccagattt aattaaggcc ttaattagg                 6769


<210>  5
<211>  1494
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2

<400>  5
atggccgccg ccgccgccgc cgcgccgagc ggaggaggag gaggaggcga ggaggagaga       60

ctggaagaaa agtcagaaga ccaggacctc cagggcctca aggacaaacc cctcaagttt      120

aaaaaggtga agaaagataa gaaagaagag aaagagggca agcatgagcc cgtgcagcca      180

tcagcccacc actctgctga gcccgcagag gcaggcaaag cagagacatc agaagggtca      240

ggctccgccc cggctgtgcc ggaagcttct gcctccccca aacagcggcg ctccatcatc      300

cgtgaccggg gacccatgta tgatgacccc accctgcctg aaggctggac acggaagctt      360

aagcaaagga aatctggccg ctctgctggg aagtatgatg tgtatttgat caatccccag      420

ggaaaagcct ttcgctctaa agtggagttg attgcgtact tcgaaaaggt aggcgacaca      480

tccctggacc ctaatgattt tgacttcacg gtaactggga gagggagccc ctcccggcga      540

gagcagaaac cacctaagaa gcccaaatct cccaaagctc caggaactgg cagaggccgg      600

ggacgcccca aagggagcgg caccacgaga cccaaggcgg ccacgtcaga gggtgtgcag      660

gtgaaaaggg tcctggagaa aagtcctggg aagctccttg tcaagatgcc ttttcaaact      720

tcgccagggg gcaaggctga ggggggtggg gccaccacat ccacccaggt catggtgatc      780

aaacgccccg gcaggaagcg aaaagctgag gccgaccctc aggccattcc caagaaacgg      840

ggccgaaagc cggggagtgt ggtggcagcc gctgccgccg aggccaaaaa gaaagccgtg      900

aaggagtctt ctatccgatc tgtgcaggag accgtactcc ccatcaagaa gcgcaagacc      960

cgggagacgg tcagcatcga ggtcaaggaa gtggtgaagc ccctgctggt gtccaccctc     1020

ggtgagaaga gcgggaaagg actgaagacc tgtaagagcc ctgggcggaa aagcaaggag     1080

agcagcccca aggggcgcag cagcagcgcc tcctcacccc ccaagaagga gcaccaccac     1140

catcaccacc actcagagtc cccaaaggcc cccgtgccac tgctcccacc cctgccccca     1200

cctccacctg agcccgagag ctccgaggac cccaccagcc cccctgagcc ccaggacttg     1260

agcagcagcg tctgcaaaga ggagaagatg cccagaggag gctcactgga gagcgacggc     1320

tgccccaagg agccagctaa gactcagccc gcggttgcca ccgccgccac ggccgcagaa     1380

aagtacaaac accgagggga gggagagcgc aaagacattg tttcatcctc catgccaagg     1440

ccaaacagag aggagcctgt ggacagccgg acgcccgtga ccgagagagt tagc           1494


<210>  6
<211>  6060
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Production plasmid 3


<220>
<221>  repeat_region
<222>  (1)..(130)
<223>  ITR

<220>
<221>  promoter
<222>  (213)..(678)
<223>  hSynapsin promoter

<220>
<221>  misc_feature
<222>  (690)..(695)
<223>  Kozak

<220>
<221>  misc_feature
<222>  (696)..(2195)
<223>  human MeCP2 coding sequence

<220>
<221>  misc_feature
<222>  (2190)..(2195)
<223>  stop codons

<220>
<221>  misc_feature
<222>  (2256)..(2277)
<223>  miRNA183

<220>
<221>  misc_feature
<222>  (2282)..(2303)
<223>  miRNA183

<220>
<221>  misc_feature
<222>  (2310)..(2331)
<223>  miRNA183

<220>
<221>  misc_feature
<222>  (2338)..(2359)
<223>  miRNA183

<220>
<221>  polyA_signal
<222>  (2377)..(2608)
<223>  SV40 late polyadenylation signal

<220>
<221>  repeat_region
<222>  (2673)..(2802)
<223>  ITR

<220>
<221>  misc_feature
<222>  (2979)..(3417)
<223>  f1 ori

<220>
<221>  misc_feature
<222>  (3446)..(4088)
<223>  pUC origin of replication

<220>
<221>  misc_feature
<222>  (4763)..(5578)
<223>  Kan-r

<220>
<221>  misc_feature
<222>  (5821)..(6006)
<223>  Lac promoter

<400>  6
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct      180

aggaagatcc tctagaacta tagctagcat gcctgcagag ggccctgcgt atgagtgcaa      240

gtgggtttta ggaccaggat gaggcggggt gggggtgcct acctgacgac cgaccccgac      300

ccactggaca agcacccaac ccccattccc caaattgcgc atcccctatc agagaggggg      360

aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc gcggacagtg      420

ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg      480

tcactcgccg gtcccccgca aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc      540

gccggcccag ccggaccgca ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat      600

ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc      660

gtgtcgtgcc tgagagcgca gtcgaattcg ccaccatggc tgctgcagct gctgctgcac      720

cttctggtgg tggcggaggc ggagaggaag agagactgga agagaagtcc gaggaccagg      780

atctgcaggg cctgaaggac aagcccctga agttcaagaa agtgaagaag gataagaaag      840

aggaaaaaga gggcaagcac gagcccgtgc agccatctgc tcaccattct gccgaacctg      900

ccgaagccgg aaaggccgaa acatctgaag gctctggaag cgcccctgcc gtgcctgaag      960

cttctgcttc tcctaagcag cggcggagca tcatcagaga cagaggccct atgtacgacg     1020

accccacact gcctgaaggc tggaccagaa agctgaagca gagaaagagc ggcagaagcg     1080

ccgggaagta cgacgtgtac ctgatcaacc ctcagggcaa agccttccgg tccaaggtgg     1140

aactgatcgc ctacttcgag aaagtgggcg acacctctct ggaccccaac gacttcgatt     1200

tcaccgtgac aggcagaggc agccccagca gaagagagca gaagcctcct aagaagccta     1260

agagccccaa ggctcctggc accggaagag gtagaggtag acctaaaggc agcggcacca     1320

caagacctaa ggccgctact tctgagggcg tgcaagtgaa gagagtgctg gaaaagagcc     1380

ccggcaagct gctggtcaag atgccctttc agacaagccc tggcggcaaa gcagaaggcg     1440

gaggggctac aacaagcacc caagtgatgg tcatcaagag gcccggcaga aagcggaagg     1500

ccgaagctga tcctcaggct atccccaaga agagaggccg gaagcctgga tctgtggttg     1560

ctgctgccgc cgcagaggcc aagaaaaagg ccgtgaaaga gtccagcatc cgcagcgtgc     1620

aagagacagt gctgcccatc aagaagcgca agacccggga aaccgtgtcc atcgaagtga     1680

aagaggtggt caagcctctg ctggtgtcta ccctgggcga gaagtctggc aagggactga     1740

aaacctgcaa gtcccctggc agaaagagca aagagtctag ccctaagggc agaagcagca     1800

gcgctagctc cccacctaag aaagaacacc accatcacca ccaccatagc gagtccccaa     1860

aggctccagt tcctctgctt cctccactgc ctccacctcc acctgagcct gagtctagcg     1920

aggatcctac aagccctcct gagccacagg atctgagcag ctccgtgtgc aaagaagaga     1980

agatgcccag aggcggctcc ctggaatctg acggctgtcc taaagagccc gccaagacac     2040

agcctgccgt tgccacagct gcaacagctg ccgagaagta caagcacaga ggcgagggcg     2100

agcgcaagga tatcgtgtct agcagcatgc ccagacctaa ccgcgaggaa cccgtggata     2160

gcagaacccc tgtgaccgag agggtgtcct gatgactcga cggcgcgccc atggcgatat     2220

ccaattggag ctcgcggccg cctcgactta ccggtagtga attctaccag tgccatagga     2280

tagtgaattc taccagtgcc atacacgtga gtgaattcta ccagtgccat agcatgcagt     2340

gaattctacc agtgccatag gtaccggcgg ccgcttcgag cagacatgat aagatacatt     2400

gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt     2460

tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac     2520

aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag     2580

taaaacctct acaaatgtgg taaaatcgat aaggatcttc ctagagcatg gctacgtaga     2640

taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac     2700

tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc     2760

gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agccttaatt aacctaattc     2820

actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg     2880

ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg     2940

cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt     3000

aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc     3060

gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca     3120

agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc     3180

caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt     3240

tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac     3300

aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc     3360

ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatcat     3420

gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt     3480

ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg     3540

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc     3600

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt     3660

ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa     3720

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta     3780

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa     3840

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa     3900

ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt     3960

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt     4020

ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat     4080

cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat     4140

gagattatca aaaaggatct tcacctagat ccttttgatc ctccggcgtt cagcctgtgc     4200

cacagccgac aggatggtga ccaccatttg ccccatatca ccgtcggtac tgatcccgtc     4260

gtcaataaac cgaaccgcta caccctgagc atcaaactct tttatcagtt ggatcatgtc     4320

ggcggtgtcg cggccaagac ggtcgagctt cttcaccaga atgacatcac cttcctccac     4380

cttcatcctc agcaaatcca gcccttcccg atctgttgaa ctgccggatg ccttgtcggt     4440

aaagatgcgg ttagctttta cccctgcatc tttgagcgct gaggtctgcc tcgtgaagaa     4500

ggtgttgctg actcatacca ggcctgaatc gccccatcat ccagccagaa agtgagggag     4560

ccacggttga tgagagcttt gttgtaggtg gaccagttgg tgattttgaa cttttgcttt     4620

gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa ctcagcaaaa     4680

gttcgattta ttcaacaaag ccgccgtccc gtcaagtcag cgtaatgctc tgccagtgtt     4740

acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt     4800

tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag     4860

aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga     4920

ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg     4980

agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta tgcatttctt     5040

tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca     5100

aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag     5160

gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa     5220

tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg     5280

cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag     5340

gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc     5400

tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga     5460

ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat     5520

ccatgttgga atttaatcgc ggcctcgagc aagacgtttc ccgttgaata tggctcataa     5580

caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt     5640

tatcttgtgc aatgtaacat cagagatttt gagacaccat gttctttcct gcgttatccc     5700

ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc     5760

gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac     5820

cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact     5880

ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc     5940

aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat     6000

ttcacacagg aaacagctat gaccatgatt acgccagatt taattaaggc cttaattagg     6060


<210>  7
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR183 binding site

<400>  7
agtgaattct accagtgcca ta                                                22


<210>  8
<211>  486
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hMECP2 Isoform A

<400>  8

Met Val Ala Gly Met Leu Gly Leu Arg Glu Glu Lys Ser Glu Asp Gln 
1               5                   10                  15      


Asp Leu Gln Gly Leu Lys Asp Lys Pro Leu Lys Phe Lys Lys Val Lys 
            20                  25                  30          


Lys Asp Lys Lys Glu Glu Lys Glu Gly Lys His Glu Pro Val Gln Pro 
        35                  40                  45              


Ser Ala His His Ser Ala Glu Pro Ala Glu Ala Gly Lys Ala Glu Thr 
    50                  55                  60                  


Ser Glu Gly Ser Gly Ser Ala Pro Ala Val Pro Glu Ala Ser Ala Ser 
65                  70                  75                  80  


Pro Lys Gln Arg Arg Ser Ile Ile Arg Asp Arg Gly Pro Met Tyr Asp 
                85                  90                  95      


Asp Pro Thr Leu Pro Glu Gly Trp Thr Arg Lys Leu Lys Gln Arg Lys 
            100                 105                 110         


Ser Gly Arg Ser Ala Gly Lys Tyr Asp Val Tyr Leu Ile Asn Pro Gln 
        115                 120                 125             


Gly Lys Ala Phe Arg Ser Lys Val Glu Leu Ile Ala Tyr Phe Glu Lys 
    130                 135                 140                 


Val Gly Asp Thr Ser Leu Asp Pro Asn Asp Phe Asp Phe Thr Val Thr 
145                 150                 155                 160 


Gly Arg Gly Ser Pro Ser Arg Arg Glu Gln Lys Pro Pro Lys Lys Pro 
                165                 170                 175     


Lys Ser Pro Lys Ala Pro Gly Thr Gly Arg Gly Arg Gly Arg Pro Lys 
            180                 185                 190         


Gly Ser Gly Thr Thr Arg Pro Lys Ala Ala Thr Ser Glu Gly Val Gln 
        195                 200                 205             


Val Lys Arg Val Leu Glu Lys Ser Pro Gly Lys Leu Leu Val Lys Met 
    210                 215                 220                 


Pro Phe Gln Thr Ser Pro Gly Gly Lys Ala Glu Gly Gly Gly Ala Thr 
225                 230                 235                 240 


Thr Ser Thr Gln Val Met Val Ile Lys Arg Pro Gly Arg Lys Arg Lys 
                245                 250                 255     


Ala Glu Ala Asp Pro Gln Ala Ile Pro Lys Lys Arg Gly Arg Lys Pro 
            260                 265                 270         


Gly Ser Val Val Ala Ala Ala Ala Ala Glu Ala Lys Lys Lys Ala Val 
        275                 280                 285             


Lys Glu Ser Ser Ile Arg Ser Val Gln Glu Thr Val Leu Pro Ile Lys 
    290                 295                 300                 


Lys Arg Lys Thr Arg Glu Thr Val Ser Ile Glu Val Lys Glu Val Val 
305                 310                 315                 320 


Lys Pro Leu Leu Val Ser Thr Leu Gly Glu Lys Ser Gly Lys Gly Leu 
                325                 330                 335     


Lys Thr Cys Lys Ser Pro Gly Arg Lys Ser Lys Glu Ser Ser Pro Lys 
            340                 345                 350         


Gly Arg Ser Ser Ser Ala Ser Ser Pro Pro Lys Lys Glu His His His 
        355                 360                 365             


His His His His Ser Glu Ser Pro Lys Ala Pro Val Pro Leu Leu Pro 
    370                 375                 380                 


Pro Leu Pro Pro Pro Pro Pro Glu Pro Glu Ser Ser Glu Asp Pro Thr 
385                 390                 395                 400 


Ser Pro Pro Glu Pro Gln Asp Leu Ser Ser Ser Val Cys Lys Glu Glu 
                405                 410                 415     


Lys Met Pro Arg Gly Gly Ser Leu Glu Ser Asp Gly Cys Pro Lys Glu 
            420                 425                 430         


Pro Ala Lys Thr Gln Pro Ala Val Ala Thr Ala Ala Thr Ala Ala Glu 
        435                 440                 445             


Lys Tyr Lys His Arg Gly Glu Gly Glu Arg Lys Asp Ile Val Ser Ser 
    450                 455                 460                 


Ser Met Pro Arg Pro Asn Arg Glu Glu Pro Val Asp Ser Arg Thr Pro 
465                 470                 475                 480 


Val Thr Glu Arg Val Ser 
                485     


<210>  9
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miRNA target sequence.

<400>  9
agcaaaaatg tgctagtgcc aaa                                               23


<210>  10
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miRNA target sequence.

<400>  10
agtgtgagtt ctaccattgc caaa                                              24


<210>  11
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miRNA target sequence.

<400>  11
agggattcct gggaaaactg gac                                               23


<210>  12
<211>  666
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chicken beta actin promoter with a cytomegalovirus enhancer (CB7)

<400>  12
ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc       60

atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac      120

cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa      180

tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc cacttggcag      240

tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc      300

ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct      360

acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc      420

ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg      480

cagcgatggg ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg      540

ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa      600

agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc      660

gggcgg                                                                 666


<210>  13
<211>  1180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human elongation initiation factor 1 alpha promoter (EF1a)

<400>  13
tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg       60

gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag      120

tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc      180

agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc      240

cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat      300

tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg      360

ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg      420

gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc      480

tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg      540

gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc      600

cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg      660

agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc      720

tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc      780

agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag      840

gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc      900

gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga      960

ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat     1020

ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta     1080

attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac     1140

agtggttcaa agtttttttc ttccatttca ggtgtcgtga                           1180


<210>  14
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spacer

<400>  14
atgacttaaa ccaggt                                                       16


<210>  15
<211>  2802
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression vector


<220>
<221>  repeat_region
<222>  (1)..(130)
<223>  5'ITR

<220>
<221>  promoter
<222>  (213)..(678)
<223>  hSynapsin

<220>
<221>  misc_feature
<222>  (690)..(695)
<223>  Kozak

<220>
<221>  misc_feature
<222>  (696)..(2195)
<223>  human Mecp2alpha

<220>
<221>  misc_binding
<222>  (2256)..(2359)
<223>  (4x)

<220>
<221>  polyA_signal
<222>  (2377)..(2608)
<223>  SV40 late polyadenylation signal

<220>
<221>  repeat_region
<222>  (2673)..(2802)
<223>  3'ITR

<400>  15
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct      180

aggaagatcc tctagaacta tagctagcat gcctgcagag ggccctgcgt atgagtgcaa      240

gtgggtttta ggaccaggat gaggcggggt gggggtgcct acctgacgac cgaccccgac      300

ccactggaca agcacccaac ccccattccc caaattgcgc atcccctatc agagaggggg      360

aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc gcggacagtg      420

ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg      480

tcactcgccg gtcccccgca aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc      540

gccggcccag ccggaccgca ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat      600

ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc      660

gtgtcgtgcc tgagagcgca gtcgaattcg ccaccatggc tgctgcagct gctgctgcac      720

cttctggtgg tggcggaggc ggagaggaag agagactgga agagaagtcc gaggaccagg      780

atctgcaggg cctgaaggac aagcccctga agttcaagaa agtgaagaag gataagaaag      840

aggaaaaaga gggcaagcac gagcccgtgc agccatctgc tcaccattct gccgaacctg      900

ccgaagccgg aaaggccgaa acatctgaag gctctggaag cgcccctgcc gtgcctgaag      960

cttctgcttc tcctaagcag cggcggagca tcatcagaga cagaggccct atgtacgacg     1020

accccacact gcctgaaggc tggaccagaa agctgaagca gagaaagagc ggcagaagcg     1080

ccgggaagta cgacgtgtac ctgatcaacc ctcagggcaa agccttccgg tccaaggtgg     1140

aactgatcgc ctacttcgag aaagtgggcg acacctctct ggaccccaac gacttcgatt     1200

tcaccgtgac aggcagaggc agccccagca gaagagagca gaagcctcct aagaagccta     1260

agagccccaa ggctcctggc accggaagag gtagaggtag acctaaaggc agcggcacca     1320

caagacctaa ggccgctact tctgagggcg tgcaagtgaa gagagtgctg gaaaagagcc     1380

ccggcaagct gctggtcaag atgccctttc agacaagccc tggcggcaaa gcagaaggcg     1440

gaggggctac aacaagcacc caagtgatgg tcatcaagag gcccggcaga aagcggaagg     1500

ccgaagctga tcctcaggct atccccaaga agagaggccg gaagcctgga tctgtggttg     1560

ctgctgccgc cgcagaggcc aagaaaaagg ccgtgaaaga gtccagcatc cgcagcgtgc     1620

aagagacagt gctgcccatc aagaagcgca agacccggga aaccgtgtcc atcgaagtga     1680

aagaggtggt caagcctctg ctggtgtcta ccctgggcga gaagtctggc aagggactga     1740

aaacctgcaa gtcccctggc agaaagagca aagagtctag ccctaagggc agaagcagca     1800

gcgctagctc cccacctaag aaagaacacc accatcacca ccaccatagc gagtccccaa     1860

aggctccagt tcctctgctt cctccactgc ctccacctcc acctgagcct gagtctagcg     1920

aggatcctac aagccctcct gagccacagg atctgagcag ctccgtgtgc aaagaagaga     1980

agatgcccag aggcggctcc ctggaatctg acggctgtcc taaagagccc gccaagacac     2040

agcctgccgt tgccacagct gcaacagctg ccgagaagta caagcacaga ggcgagggcg     2100

agcgcaagga tatcgtgtct agcagcatgc ccagacctaa ccgcgaggaa cccgtggata     2160

gcagaacccc tgtgaccgag agggtgtcct gatgactcga cggcgcgccc atggcgatat     2220

ccaattggag ctcgcggccg cctcgactta ccggtagtga attctaccag tgccatagga     2280

tagtgaattc taccagtgcc atacacgtga gtgaattcta ccagtgccat agcatgcagt     2340

gaattctacc agtgccatag gtaccggcgg ccgcttcgag cagacatgat aagatacatt     2400

gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt     2460

tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac     2520

aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag     2580

taaaacctct acaaatgtgg taaaatcgat aaggatcttc ctagagcatg gctacgtaga     2640

taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac     2700

tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc     2760

gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc ag                        2802


<210>  16
<211>  2026
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mRNA transcript variant

<400>  16
ccggcgtcgg cggcgcgcgc gctccctcct ctcggagaga gggctgtggt aaaagccgtc       60

cggaaaatgg ccgccgccgc cgccgccgcg ccgagcggag gaggaggagg aggcgaggag      120

gagagactgc tccataaaaa tacagactca ccagttcctg ctttgatgtg acatgtgact      180

ccccagaata caccttgctt ctgtagacca gctccaacag gattccatgg tagctgggat      240

gttagggctc agctgcaaga tgggattcag atctgttctc aagcctgtcg ttccaggacc      300

caggagggag aagcagctgc caggggaagt ctcttcgtag gcggaggtca ggagtccaag      360

aggagtgagc agagtcacag aagcctctta aagcctcttc ttcccccatc ccatcaacac      420

ggaagaaaag tcagaagacc aggacctcca gggcctcaag gacaaacccc tcaagtttaa      480

aaaggtgaag aaagataaga aagaagagaa agagggcaag catgagcccg tgcagccatc      540

agcccaccac tctgctgagc ccgcagaggc aggcaaagca gagacatcag aagggtcagg      600

ctccgccccg gctgtgccgg aagcttctgc ctcccccaaa cagcggcgct ccatcatccg      660

tgaccgggga cccatgtatg atgaccccac cctgcctgaa ggctggacac ggaagcttaa      720

gcaaaggaaa tctggccgct ctgctgggaa gtatgatgtg tatttgatca atccccaggg      780

aaaagccttt cgctctaaag tggagttgat tgcgtacttc gaaaaggtag gcgacacatc      840

cctggaccct aatgattttg acttcacggt aactgggaga gggagcccct cccggcgaga      900

gcagaaacca cctaagaagc ccaaatctcc caaagctcca ggaactggca gaggccgggg      960

acgccccaaa gggagcggca ccacgagacc caaggcggcc acgtcagagg gtgtgcaggt     1020

gaaaagggtc ctggagaaaa gtcctgggaa gctccttgtc aagatgcctt ttcaaacttc     1080

gccagggggc aaggctgagg ggggtggggc caccacatcc acccaggtca tggtgatcaa     1140

acgccccggc aggaagcgaa aagctgaggc cgaccctcag gccattccca agaaacgggg     1200

ccgaaagccg gggagtgtgg tggcagccgc tgccgccgag gccaaaaaga aagccgtgaa     1260

ggagtcttct atccgatctg tgcaggagac cgtactcccc atcaagaagc gcaagacccg     1320

ggagacggtc agcatcgagg tcaaggaagt ggtgaagccc ctgctggtgt ccaccctcgg     1380

tgagaagagc gggaaaggac tgaagacctg taagagccct gggcggaaaa gcaaggagag     1440

cagccccaag gggcgcagca gcagcgcctc ctcacccccc aagaaggagc accaccacca     1500

tcaccaccac tcagagtccc caaaggcccc cgtgccactg ctcccacccc tgcccccacc     1560

tccacctgag cccgagagct ccgaggaccc caccagcccc cctgagcccc aggacttgag     1620

cagcagcgtc tgcaaagagg agaagatgcc cagaggaggc tcactggaga gcgacggctg     1680

ccccaaggag ccagctaaga ctcagcccgc ggttgccacc gccgccacgg ccgcagaaaa     1740

gtacaaacac cgaggggagg gagagcgcaa agacattgtt tcatcctcca tgccaaggcc     1800

aaacagagag gagcctgtgg acagccggac gcccgtgacc gagagagtta gctgacttta     1860

cacggagcgg attgcaaagc aaaccaacaa gaataaaggc agctgttgtc tcttctcctt     1920

atgggtaggg ctctgacaaa gcttcccgat taactgaaat aaaaaatatt tttttttctt     1980

tcagtaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa                    2026


<210>  17
<211>  10241
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mRNA transcript variant

<400>  17
ccggcgtcgg cggcgcgcgc gctccctcct ctcggagaga gggctgtggt aaaagccgtc       60

cggaaaatgg ccgccgccgc cgccgccgcg ccgagcggag gaggaggagg aggcgaggag      120

gagagactgc tccataaaaa tacagactca ccagttcctg ctttgatgtg acatgtgact      180

ccccagaata caccttgctt ctgtagacca gctccaacag gattccatgg tagctgggat      240

gttagggctc agggaagaaa agtcagaaga ccaggacctc cagggcctca aggacaaacc      300

cctcaagttt aaaaaggtga agaaagataa gaaagaagag aaagagggca agcatgagcc      360

cgtgcagcca tcagcccacc actctgctga gcccgcagag gcaggcaaag cagagacatc      420

agaagggtca ggctccgccc cggctgtgcc ggaagcttct gcctccccca aacagcggcg      480

ctccatcatc cgtgaccggg gacccatgta tgatgacccc accctgcctg aaggctggac      540

acggaagctt aagcaaagga aatctggccg ctctgctggg aagtatgatg tgtatttgat      600

caatccccag ggaaaagcct ttcgctctaa agtggagttg attgcgtact tcgaaaaggt      660

aggcgacaca tccctggacc ctaatgattt tgacttcacg gtaactggga gagggagccc      720

ctcccggcga gagcagaaac cacctaagaa gcccaaatct cccaaagctc caggaactgg      780

cagaggccgg ggacgcccca aagggagcgg caccacgaga cccaaggcgg ccacgtcaga      840

gggtgtgcag gtgaaaaggg tcctggagaa aagtcctggg aagctccttg tcaagatgcc      900

ttttcaaact tcgccagggg gcaaggctga ggggggtggg gccaccacat ccacccaggt      960

catggtgatc aaacgccccg gcaggaagcg aaaagctgag gccgaccctc aggccattcc     1020

caagaaacgg ggccgaaagc cggggagtgt ggtggcagcc gctgccgccg aggccaaaaa     1080

gaaagccgtg aaggagtctt ctatccgatc tgtgcaggag accgtactcc ccatcaagaa     1140

gcgcaagacc cgggagacgg tcagcatcga ggtcaaggaa gtggtgaagc ccctgctggt     1200

gtccaccctc ggtgagaaga gcgggaaagg actgaagacc tgtaagagcc ctgggcggaa     1260

aagcaaggag agcagcccca aggggcgcag cagcagcgcc tcctcacccc ccaagaagga     1320

gcaccaccac catcaccacc actcagagtc cccaaaggcc cccgtgccac tgctcccacc     1380

cctgccccca cctccacctg agcccgagag ctccgaggac cccaccagcc cccctgagcc     1440

ccaggacttg agcagcagcg tctgcaaaga ggagaagatg cccagaggag gctcactgga     1500

gagcgacggc tgccccaagg agccagctaa gactcagccc gcggttgcca ccgccgccac     1560

ggccgcagaa aagtacaaac accgagggga gggagagcgc aaagacattg tttcatcctc     1620

catgccaagg ccaaacagag aggagcctgt ggacagccgg acgcccgtga ccgagagagt     1680

tagctgactt tacacggagc ggattgcaaa gcaaaccaac aagaataaag gcagctgttg     1740

tctcttctcc ttatgggtag ggctctgaca aagcttcccg attaactgaa ataaaaaata     1800

tttttttttc tttcagtaaa cttagagttt cgtggcttca gggtgggagt agttggagca     1860

ttggggatgt ttttcttacc gacaagcaca gtcaggttga agacctaacc agggccagaa     1920

gtagctttgc acttttctaa actaggctcc ttcaacaagg cttgctgcag atactactga     1980

ccagacaagc tgttgaccag gcacctcccc tcccgcccaa acctttcccc catgtggtcg     2040

ttagagacag agcgacagag cagttgagag gacactcccg ttttcggtgc catcagtgcc     2100

ccgtctacag ctcccccagc tccccccacc tcccccactc ccaaccacgt tgggacaggg     2160

aggtgtgagg caggagagac agttggattc tttagagaag atggatatga ccagtggcta     2220

tggcctgtgc gatcccaccc gtggtggctc aagtctggcc ccacaccagc cccaatccaa     2280

aactggcaag gacgcttcac aggacaggaa agtggcacct gtctgctcca gctctggcat     2340

ggctaggagg ggggagtccc ttgaactact gggtgtagac tggcctgaac cacaggagag     2400

gatggcccag ggtgaggtgg catggtccat tctcaaggga cgtcctccaa cgggtggcgc     2460

tagaggccat ggaggcagta ggacaaggtg caggcaggct ggcctggggt caggccgggc     2520

agagcacagc ggggtgagag ggattcctaa tcactcagag cagtctgtga cttagtggac     2580

aggggagggg gcaaaggggg aggagaagaa aatgttcttc cagttacttt ccaattctcc     2640

tttagggaca gcttagaatt atttgcacta ttgagtcttc atgttcccac ttcaaaacaa     2700

acagatgctc tgagagcaaa ctggcttgaa ttggtgacat ttagtccctc aagccaccag     2760

atgtgacagt gttgagaact acctggattt gtatatatac ctgcgcttgt tttaaagtgg     2820

gctcagcaca tagggttccc acgaagctcc gaaactctaa gtgtttgctg caattttata     2880

aggacttcct gattggtttc tcttctcccc ttccatttct gccttttgtt catttcatcc     2940

tttcacttct ttcccttcct ccgtcctcct ccttcctagt tcatcccttc tcttccaggc     3000

agccgcggtg cccaaccaca cttgtcggct ccagtcccca gaactctgcc tgccctttgt     3060

cctcctgctg ccagtaccag ccccaccctg ttttgagccc tgaggaggcc ttgggctctg     3120

ctgagtccga cctggcctgt ctgtgaagag caagagagca gcaaggtctt gctctcctag     3180

gtagccccct cttccctggt aagaaaaagc aaaaggcatt tcccaccctg aacaacgagc     3240

cttttcaccc ttctactcta gagaagtgga ctggaggagc tgggcccgat ttggtagttg     3300

aggaaagcac agaggcctcc tgtggcctgc cagtcatcga gtggcccaac aggggctcca     3360

tgccagccga ccttgacctc actcagaagt ccagagtcta gcgtagtgca gcagggcagt     3420

agcggtacca atgcagaact cccaagaccc gagctgggac cagtacctgg gtccccagcc     3480

cttcctctgc tccccctttt ccctcggagt tcttcttgaa tggcaatgtt ttgcttttgc     3540

tcgatgcaga cagggggcca gaacaccaca catttcactg tctgtctggt ccatagctgt     3600

ggtgtagggg cttagaggca tgggcttgct gtgggttttt aattgatcag ttttcatgtg     3660

ggatcccatc tttttaacct ctgttcagga agtccttatc tagctgcata tcttcatcat     3720

attggtatat ccttttctgt gtttacagag atgtctctta tatctaaatc tgtccaactg     3780

agaagtacct tatcaaagta gcaaatgaga cagcagtctt atgcttccag aaacacccac     3840

aggcatgtcc catgtgagct gctgccatga actgtcaagt gtgtgttgtc ttgtgtattt     3900

cagttattgt ccctggcttc cttactatgg tgtaatcatg aaggagtgaa acatcataga     3960

aactgtctag cacttccttg ccagtcttta gtgatcagga accatagttg acagttccaa     4020

tcagtagctt aagaaaaaac cgtgtttgtc tcttctggaa tggttagaag tgagggagtt     4080

tgccccgttc tgtttgtaga gtctcatagt tggactttct agcatatatg tgtccatttc     4140

cttatgctgt aaaagcaagt cctgcaacca aactcccatc agcccaatcc ctgatccctg     4200

atcccttcca cctgctctgc tgatgacccc cccagcttca cttctgactc ttccccagga     4260

agggaagggg ggtcagaaga gagggtgagt cctccagaac tcttcctcca aggacagaag     4320

gctcctgccc ccatagtggc ctcgaactcc tggcactacc aaaggacact tatccacgag     4380

agcgcagcat ccgaccaggt tgtcactgag aagatgttta ttttggtcag ttgggttttt     4440

atgtattata cttagtcaaa tgtaatgtgg cttctggaat cattgtccag agctgcttcc     4500

ccgtcacctg ggcgtcatct ggtcctggta agaggagtgc gtggcccacc aggcccccct     4560

gtcacccatg acagttcatt cagggccgat ggggcagtcg tggttgggaa cacagcattt     4620

caagcgtcac tttatttcat tcgggcccca cctgcagctc cctcaaagag gcagttgccc     4680

agcctctttc ccttccagtt tattccagag ctgccagtgg ggcctgaggc tccttagggt     4740

tttctctcta tttccccctt tcttcctcat tccctcgtct ttcccaaagg catcacgagt     4800

cagtcgcctt tcagcaggca gccttggcgg tttatcgccc tggcaggcag gggccctgca     4860

gctctcatgc tgcccctgcc ttggggtcag gttgacagga ggttggaggg aaagccttaa     4920

gctgcaggat tctcaccagc tgtgtccggc ccagttttgg ggtgtgacct caatttcaat     4980

tttgtctgta cttgaacatt atgaagatgg gggcctcttt cagtgaattt gtgaacagca     5040

gaattgaccg acagctttcc agtacccatg gggctaggtc attaaggcca catccacagt     5100

ctcccccacc cttgttccag ttgttagtta ctacctcctc tcctgacaat actgtatgtc     5160

gtcgagctcc ccccaggtct acccctcccg gccctgcctg ctggtgggct tgtcatagcc     5220

agtgggattg ccggtcttga cagctcagtg agctggagat acttggtcac agccaggcgc     5280

tagcacagct cccttctgtt gatgctgtat tcccatatca aaagacacag gggacaccca     5340

gaaacgccac atcccccaat ccatcagtgc caaactagcc aacggcccca gcttctcagc     5400

tcgctggatg gcggaagctg ctactcgtga gcgccagtgc gggtgcagac aatcttctgt     5460

tgggtggcat cattccaggc ccgaagcatg aacagtgcac ctgggacagg gagcagcccc     5520

aaattgtcac ctgcttctct gcccagcttt tcattgctgt gacagtgatg gcgaaagagg     5580

gtaataacca gacacaaact gccaagttgg gtggagaaag gagtttcttt agctgacaga     5640

atctctgaat tttaaatcac ttagtaagcg gctcaagccc aggagggagc agagggatac     5700

gagcggagtc ccctgcgcgg gaccatctgg aattggttta gcccaagtgg agcctgacag     5760

ccagaactct gtgtcccccg tctaaccaca gctccttttc cagagcattc cagtcaggct     5820

ctctgggctg actgggccag gggaggttac aggtaccagt tctttaagaa gatctttggg     5880

catatacatt tttagcctgt gtcattgccc caaatggatt cctgtttcaa gttcacacct     5940

gcagattcta ggacctgtgt cctagacttc agggagtcag ctgtttctag agttcctacc     6000

atggagtggg tctggaggac ctgcccggtg ggggggcaga gccctgctcc ctccgggtct     6060

tcctactctt ctctctgctc tgacgggatt tgttgattct ctccattttg gtgtctttct     6120

cttttagata ttgtatcaat ctttagaaaa ggcatagtct acttgttata aatcgttagg     6180

atactgcctc ccccagggtc taaaattaca tattagaggg gaaaagctga acactgaagt     6240

cagttctcaa caatttagaa ggaaaaccta gaaaacattt ggcagaaaat tacatttcga     6300

tgtttttgaa tgaatacgag caagctttta caacagtgct gatctaaaaa tacttagcac     6360

ttggcctgag atgcctggtg agcattacag gcaaggggaa tctggaggta gccgacctga     6420

ggacatggct tctgaacctg tcttttggga gtggtatgga aggtggagcg ttcaccagtg     6480

acctggaagg cccagcacca ccctccttcc cactcttctc atcttgacag agcctgcccc     6540

agcgctgacg tgtcaggaaa acacccaggg aactaggaag gcacttctgc ctgaggggca     6600

gcctgccttg cccactcctg ctctgctcgc ctcggatcag ctgagccttc tgagctggcc     6660

tctcactgcc tccccaaggc cccctgcctg ccctgtcagg aggcagaagg aagcaggtgt     6720

gagggcagtg caaggaggga gcacaacccc cagctcccgc tccgggctcc gacttgtgca     6780

caggcagagc ccagaccctg gaggaaatcc tacctttgaa ttcaagaaca tttggggaat     6840

ttggaaatct ctttgccccc aaacccccat tctgtcctac ctttaatcag gtcctgctca     6900

gcagtgagag cagatgaggt gaaaaggcca agaggtttgg ctcctgccca ctgatagccc     6960

ctctccccgc agtgtttgtg tgtcaagtgg caaagctgtt cttcctggtg accctgatta     7020

tatccagtaa cacatagact gtgcgcatag gcctgctttg tctcctctat cctgggcttt     7080

tgttttgctt tttagttttg cttttagttt ttctgtccct tttatttaac gcaccgacta     7140

gacacacaaa gcagttgaat ttttatatat atatctgtat attgcacaat tataaactca     7200

ttttgcttgt ggctccacac acacaaaaaa agacctgtta aaattatacc tgttgcttaa     7260

ttacaatatt tctgataacc atagcatagg acaagggaaa ataaaaaaag aaaaaaaaga     7320

aaaaaaaacg acaaatctgt ctgctggtca cttcttctgt ccaagcagat tcgtggtctt     7380

ttcctcgctt ctttcaaggg ctttcctgtg ccaggtgaag gaggctccag gcagcaccca     7440

ggttttgcac tcttgtttct cccgtgcttg tgaaagaggt cccaaggttc tgggtgcagg     7500

agcgctccct tgacctgctg aagtccggaa cgtagtcggc acagcctggt cgccttccac     7560

ctctgggagc tggagtccac tggggtggcc tgactccccc agtccccttc ccgtgacctg     7620

gtcagggtga gcccatgtgg agtcagcctc gcaggcctcc ctgccagtag ggtccgagtg     7680

tgtttcatcc ttcccactct gtcgagcctg ggggctggag cggagacggg aggcctggcc     7740

tgtctcggaa cctgtgagct gcaccaggta gaacgccagg gaccccagaa tcatgtgcgt     7800

cagtccaagg ggtcccctcc aggagtagtg aagactccag aaatgtccct ttcttctccc     7860

ccatcctacg agtaattgca tttgcttttg taattcttaa tgagcaatat ctgctagaga     7920

gtttagctgt aacagttctt tttgatcatc tttttttaat aattagaaac accaaaaaaa     7980

tccagaaact tgttcttcca aagcagagag cattataatc accagggcca aaagcttccc     8040

tccctgctgt cattgcttct tctgaggcct gaatccaaaa gaaaaacagc cataggccct     8100

ttcagtggcc gggctacccg tgagcccttc ggaggaccag ggctggggca gcctctgggc     8160

ccacatccgg ggccagctcc ggcgtgtgtt cagtgttagc agtgggtcat gatgctcttt     8220

cccacccagc ctgggatagg ggcagaggag gcgaggaggc cgttgccgct gatgtttggc     8280

cgtgaacagg tgggtgtctg cgtgcgtcca cgtgcgtgtt ttctgactga catgaaatcg     8340

acgcccgagt tagcctcacc cggtgacctc tagccctgcc cggatggagc ggggcccacc     8400

cggttcagtg tttctgggga gctggacagt ggagtgcaaa aggcttgcag aacttgaagc     8460

ctgctccttc ccttgctacc acggcctcct ttccgtttga tttgtcactg cttcaatcaa     8520

taacagccgc tccagagtca gtagtcaatg aatatatgac caaatatcac caggactgtt     8580

actcaatgtg tgccgagccc ttgcccatgc tgggctcccg tgtatctgga cactgtaacg     8640

tgtgctgtgt ttgctcccct tccccttcct tctttgccct ttacttgtct ttctggggtt     8700

tttctgtttg ggtttggttt ggtttttatt tctccttttg tgttccaaac atgaggttct     8760

ctctactggt cctcttaact gtggtgttga ggcttatatt tgtgtaattt ttggtgggtg     8820

aaaggaattt tgctaagtaa atctcttctg tgtttgaact gaagtctgta ttgtaactat     8880

gtttaaagta attgttccag agacaaatat ttctagacac tttttcttta caaacaaaag     8940

cattcggagg gagggggatg gtgactgaga tgagagggga gagctgaaca gatgacccct     9000

gcccagatca gccagaagcc acccaaagca gtggagccca ggagtcccac tccaagccag     9060

caagccgaat agctgatgtg ttgccacttt ccaagtcact gcaaaaccag gttttgttcc     9120

gcccagtgga ttcttgtttt gcttcccctc cccccgagat tattaccacc atcccgtgct     9180

tttaaggaaa ggcaagattg atgtttcctt gaggggagcc aggaggggat gtgtgtgtgc     9240

agagctgaag agctggggag aatggggctg ggcccaccca agcaggaggc tgggacgctc     9300

tgctgtgggc acaggtcagg ctaatgttgg cagatgcagc tcttcctgga caggccaggt     9360

ggtgggcatt ctctctccaa ggtgtgcccc gtgggcatta ctgtttaaga cacttccgtc     9420

acatcccacc ccatcctcca gggctcaaca ctgtgacatc tctattcccc accctcccct     9480

tcccagggca ataaaatgac catggagggg gcttgcactc tcttggctgt cacccgatcg     9540

ccagcaaaac ttagatgtga gaaaacccct tcccattcca tggcgaaaac atctccttag     9600

aaaagccatt accctcatta ggcatggttt tgggctccca aaacacctga cagcccctcc     9660

ctcctctgag aggcggagag tgctgactgt agtgaccatt gcatgccggg tgcagcatct     9720

ggaagagcta ggcagggtgt ctgccccctc ctgagttgaa gtcatgctcc cctgtgccag     9780

cccagaggcc gagagctatg gacagcattg ccagtaacac aggccaccct gtgcagaagg     9840

gagctggctc cagcctggaa acctgtctga ggttgggaga ggtgcacttg gggcacaggg     9900

agaggccggg acacacttag ctggagatgt ctctaaaagc cctgtatcgt attcaccttc     9960

agtttttgtg ttttgggaca attactttag aaaataagta ggtcgtttta aaaacaaaaa    10020

ttattgattg cttttttgta gtgttcagaa aaaaggttct ttgtgtatag ccaaatgact    10080

gaaagcactg atatatttaa aaacaaaagg caatttatta aggaaatttg taccatttca    10140

gtaaacctgt ctgaatgtac ctgtatacgt ttcaaaaaca cccccccccc actgaatccc    10200

tgtaacctat ttattatata aagagtttgc cttataaatt t                        10241


<210>  18
<211>  1734
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mRNA transcript variant

<400>  18
ccggcgtcgg cggcgcgcgc gctccctcct ctcggagaga gggctgtggt aaaagccgtc       60

cggaaaatgg ccgccgccgc cgccgccgcg ccgagcggag gaggaggagg aggcgaggag      120

gagagactgg aagaaaagtc agaagaccag gacctccagg gcctcaagga caaacccctc      180

aagtttaaaa aggtgaagaa agataagaaa gaagagaaag agggcaagca tgagcccgtg      240

cagccatcag cccaccactc tgctgagccc gcagaggcag gcaaagcaga gacatcagaa      300

gggtcaggct ccgccccggc tgtgccggaa gcttctgcct cccccaaaca gcggcgctcc      360

atcatccgtg accggggacc catgtatgat gaccccaccc tgcctgaagg ctggacacgg      420

aagcttaagc aaaggaaatc tggccgctct gctgggaagt atgatgtgta tttgatcaat      480

ccccagggaa aagcctttcg ctctaaagtg gagttgattg cgtacttcga aaaggtaggc      540

gacacatccc tggaccctaa tgattttgac ttcacggtaa ctgggagagg gagcccctcc      600

cggcgagagc agaaaccacc taagaagccc aaatctccca aagctccagg aactggcaga      660

ggccggggac gccccaaagg gagcggcacc acgagaccca aggcggccac gtcagagggt      720

gtgcaggtga aaagggtcct ggagaaaagt cctgggaagc tccttgtcaa gatgcctttt      780

caaacttcgc cagggggcaa ggctgagggg ggtggggcca ccacatccac ccaggtcatg      840

gtgatcaaac gccccggcag gaagcgaaaa gctgaggccg accctcaggc cattcccaag      900

aaacggggcc gaaagccggg gagtgtggtg gcagccgctg ccgccgaggc caaaaagaaa      960

gccgtgaagg agtcttctat ccgatctgtg caggagaccg tactccccat caagaagcgc     1020

aagacccggg agacggtcag catcgaggtc aaggaagtgg tgaagcccct gctggtgtcc     1080

accctcggtg agaagagcgg gaaaggactg aagacctgta agagccctgg gcggaaaagc     1140

aaggagagca gccccaaggg gcgcagcagc agcgcctcct caccccccaa gaaggagcac     1200

caccaccatc accaccactc agagtcccca aaggcccccg tgccactgct cccacccctg     1260

cccccacctc cacctgagcc cgagagctcc gaggacccca ccagcccccc tgagccccag     1320

gacttgagca gcagcgtctg caaagaggag aagatgccca gaggaggctc actggagagc     1380

gacggctgcc ccaaggagcc agctaagact cagcccgcgg ttgccaccgc cgccacggcc     1440

gcagaaaagt acaaacaccg aggggaggga gagcgcaaag acattgtttc atcctccatg     1500

ccaaggccaa acagagagga gcctgtggac agccggacgc ccgtgaccga gagagttagc     1560

tgactttaca cggagcggat tgcaaagcaa accaacaaga ataaaggcag ctgttgtctc     1620

ttctccttat gggtagggct ctgacaaagc ttcccgatta actgaaataa aaaatatttt     1680

tttttctttc agtaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa           1734


<210>  19
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MECP2 variant

<400>  19

Met Tyr Asp Asp Pro Thr Leu Pro Glu Gly Trp Thr Arg Lys Leu Lys 
1               5                   10                  15      


Gln Arg Lys Ser Gly Arg Ser Ala Gly Lys Tyr Asp Val Tyr Leu Ile 
            20                  25                  30          


Asn Pro Gln Gly Lys Ala Phe Arg Ser Lys Val Glu Leu Ile Ala Tyr 
        35                  40                  45              


Phe Glu Lys Val Gly Asp Thr Ser Leu Asp Pro Asn Asp Phe Asp Phe 
    50                  55                  60                  


Thr Val Thr Gly Arg Gly Ser Pro Ser Arg Arg Glu Gln Lys Pro Pro 
65                  70                  75                  80  


Lys Lys Pro Lys Ser Pro Lys Ala Pro Gly Thr Gly Arg Gly Arg Gly 
                85                  90                  95      


Arg Pro Lys Gly Ser Gly Thr Thr Arg Pro Lys Ala Ala Thr Ser Glu 
            100                 105                 110         


Gly Val Gln Val Lys Arg Val Leu Glu Lys Ser Pro Gly Lys Leu Leu 
        115                 120                 125             


Val Lys Met Pro Phe Gln Thr Ser Pro Gly Gly Lys Ala Glu Gly Gly 
    130                 135                 140                 


Gly Ala Thr Thr Ser Thr Gln Val Met Val Ile Lys Arg Pro Gly Arg 
145                 150                 155                 160 


Lys Arg Lys Ala Glu Ala Asp Pro Gln Ala Ile Pro Lys Lys Arg Gly 
                165                 170                 175     


Arg Lys Pro Gly Ser Val Val Ala Ala Ala Ala Ala Glu Ala Lys Lys 
            180                 185                 190         


Lys Ala Val Lys Glu Ser Ser Ile Arg Ser Val Gln Glu Thr Val Leu 
        195                 200                 205             


Pro Ile Lys Lys Arg Lys Thr Arg Glu Thr Val Ser Ile Glu Val Lys 
    210                 215                 220                 


Glu Val Val Lys Pro Leu Leu Val Ser Thr Leu Gly Glu Lys Ser Gly 
225                 230                 235                 240 


Lys Gly Leu Lys Thr Cys Lys Ser Pro Gly Arg Lys Ser Lys Glu Ser 
                245                 250                 255     


Ser Pro Lys Gly Arg Ser Ser Ser Ala Ser Ser Pro Pro Lys Lys Glu 
            260                 265                 270         


His His His His His His His Ser Glu Ser Pro Lys Ala Pro Val Pro 
        275                 280                 285             


Leu Leu Pro Pro Leu Pro Pro Pro Pro Pro Glu Pro Glu Ser Ser Glu 
    290                 295                 300                 


Asp Pro Thr Ser Pro Pro Glu Pro Gln Asp Leu Ser Ser Ser Val Cys 
305                 310                 315                 320 


Lys Glu Glu Lys Met Pro Arg Gly Gly Ser Leu Glu Ser Asp Gly Cys 
                325                 330                 335     


Pro Lys Glu Pro Ala Lys Thr Gln Pro Ala Val Ala Thr Ala Ala Thr 
            340                 345                 350         


Ala Ala Glu Lys Tyr Lys His Arg Gly Glu Gly Glu Arg Lys Asp Ile 
        355                 360                 365             


Val Ser Ser Ser Met Pro Arg Pro Asn Arg Glu Glu Pro Val Asp Ser 
    370                 375                 380                 


Arg Thr Pro Val Thr Glu Arg Val Ser 
385                 390             


<210>  20
<211>  486
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MECP2 variant

<400>  20

Met Val Ala Gly Met Leu Gly Leu Arg Glu Glu Lys Ser Glu Asp Gln 
1               5                   10                  15      


Asp Leu Gln Gly Leu Lys Asp Lys Pro Leu Lys Phe Lys Lys Val Lys 
            20                  25                  30          


Lys Asp Lys Lys Glu Glu Lys Glu Gly Lys His Glu Pro Val Gln Pro 
        35                  40                  45              


Ser Ala His His Ser Ala Glu Pro Ala Glu Ala Gly Lys Ala Glu Thr 
    50                  55                  60                  


Ser Glu Gly Ser Gly Ser Ala Pro Ala Val Pro Glu Ala Ser Ala Ser 
65                  70                  75                  80  


Pro Lys Gln Arg Arg Ser Ile Ile Arg Asp Arg Gly Pro Met Tyr Asp 
                85                  90                  95      


Asp Pro Thr Leu Pro Glu Gly Trp Thr Arg Lys Leu Lys Gln Arg Lys 
            100                 105                 110         


Ser Gly Arg Ser Ala Gly Lys Tyr Asp Val Tyr Leu Ile Asn Pro Gln 
        115                 120                 125             


Gly Lys Ala Phe Arg Ser Lys Val Glu Leu Ile Ala Tyr Phe Glu Lys 
    130                 135                 140                 


Val Gly Asp Thr Ser Leu Asp Pro Asn Asp Phe Asp Phe Thr Val Thr 
145                 150                 155                 160 


Gly Arg Gly Ser Pro Ser Arg Arg Glu Gln Lys Pro Pro Lys Lys Pro 
                165                 170                 175     


Lys Ser Pro Lys Ala Pro Gly Thr Gly Arg Gly Arg Gly Arg Pro Lys 
            180                 185                 190         


Gly Ser Gly Thr Thr Arg Pro Lys Ala Ala Thr Ser Glu Gly Val Gln 
        195                 200                 205             


Val Lys Arg Val Leu Glu Lys Ser Pro Gly Lys Leu Leu Val Lys Met 
    210                 215                 220                 


Pro Phe Gln Thr Ser Pro Gly Gly Lys Ala Glu Gly Gly Gly Ala Thr 
225                 230                 235                 240 


Thr Ser Thr Gln Val Met Val Ile Lys Arg Pro Gly Arg Lys Arg Lys 
                245                 250                 255     


Ala Glu Ala Asp Pro Gln Ala Ile Pro Lys Lys Arg Gly Arg Lys Pro 
            260                 265                 270         


Gly Ser Val Val Ala Ala Ala Ala Ala Glu Ala Lys Lys Lys Ala Val 
        275                 280                 285             


Lys Glu Ser Ser Ile Arg Ser Val Gln Glu Thr Val Leu Pro Ile Lys 
    290                 295                 300                 


Lys Arg Lys Thr Arg Glu Thr Val Ser Ile Glu Val Lys Glu Val Val 
305                 310                 315                 320 


Lys Pro Leu Leu Val Ser Thr Leu Gly Glu Lys Ser Gly Lys Gly Leu 
                325                 330                 335     


Lys Thr Cys Lys Ser Pro Gly Arg Lys Ser Lys Glu Ser Ser Pro Lys 
            340                 345                 350         


Gly Arg Ser Ser Ser Ala Ser Ser Pro Pro Lys Lys Glu His His His 
        355                 360                 365             


His His His His Ser Glu Ser Pro Lys Ala Pro Val Pro Leu Leu Pro 
    370                 375                 380                 


Pro Leu Pro Pro Pro Pro Pro Glu Pro Glu Ser Ser Glu Asp Pro Thr 
385                 390                 395                 400 


Ser Pro Pro Glu Pro Gln Asp Leu Ser Ser Ser Val Cys Lys Glu Glu 
                405                 410                 415     


Lys Met Pro Arg Gly Gly Ser Leu Glu Ser Asp Gly Cys Pro Lys Glu 
            420                 425                 430         


Pro Ala Lys Thr Gln Pro Ala Val Ala Thr Ala Ala Thr Ala Ala Glu 
        435                 440                 445             


Lys Tyr Lys His Arg Gly Glu Gly Glu Arg Lys Asp Ile Val Ser Ser 
    450                 455                 460                 


Ser Met Pro Arg Pro Asn Arg Glu Glu Pro Val Asp Ser Arg Thr Pro 
465                 470                 475                 480 


Val Thr Glu Arg Val Ser 
                485     


<210>  21
<211>  498
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MECP2 variant

<400>  21

Met Ala Ala Ala Ala Ala Ala Ala Pro Ser Gly Gly Gly Gly Gly Gly 
1               5                   10                  15      


Glu Glu Glu Arg Leu Glu Glu Lys Ser Glu Asp Gln Asp Leu Gln Gly 
            20                  25                  30          


Leu Lys Asp Lys Pro Leu Lys Phe Lys Lys Val Lys Lys Asp Lys Lys 
        35                  40                  45              


Glu Glu Lys Glu Gly Lys His Glu Pro Val Gln Pro Ser Ala His His 
    50                  55                  60                  


Ser Ala Glu Pro Ala Glu Ala Gly Lys Ala Glu Thr Ser Glu Gly Ser 
65                  70                  75                  80  


Gly Ser Ala Pro Ala Val Pro Glu Ala Ser Ala Ser Pro Lys Gln Arg 
                85                  90                  95      


Arg Ser Ile Ile Arg Asp Arg Gly Pro Met Tyr Asp Asp Pro Thr Leu 
            100                 105                 110         


Pro Glu Gly Trp Thr Arg Lys Leu Lys Gln Arg Lys Ser Gly Arg Ser 
        115                 120                 125             


Ala Gly Lys Tyr Asp Val Tyr Leu Ile Asn Pro Gln Gly Lys Ala Phe 
    130                 135                 140                 


Arg Ser Lys Val Glu Leu Ile Ala Tyr Phe Glu Lys Val Gly Asp Thr 
145                 150                 155                 160 


Ser Leu Asp Pro Asn Asp Phe Asp Phe Thr Val Thr Gly Arg Gly Ser 
                165                 170                 175     


Pro Ser Arg Arg Glu Gln Lys Pro Pro Lys Lys Pro Lys Ser Pro Lys 
            180                 185                 190         


Ala Pro Gly Thr Gly Arg Gly Arg Gly Arg Pro Lys Gly Ser Gly Thr 
        195                 200                 205             


Thr Arg Pro Lys Ala Ala Thr Ser Glu Gly Val Gln Val Lys Arg Val 
    210                 215                 220                 


Leu Glu Lys Ser Pro Gly Lys Leu Leu Val Lys Met Pro Phe Gln Thr 
225                 230                 235                 240 


Ser Pro Gly Gly Lys Ala Glu Gly Gly Gly Ala Thr Thr Ser Thr Gln 
                245                 250                 255     


Val Met Val Ile Lys Arg Pro Gly Arg Lys Arg Lys Ala Glu Ala Asp 
            260                 265                 270         


Pro Gln Ala Ile Pro Lys Lys Arg Gly Arg Lys Pro Gly Ser Val Val 
        275                 280                 285             


Ala Ala Ala Ala Ala Glu Ala Lys Lys Lys Ala Val Lys Glu Ser Ser 
    290                 295                 300                 


Ile Arg Ser Val Gln Glu Thr Val Leu Pro Ile Lys Lys Arg Lys Thr 
305                 310                 315                 320 


Arg Glu Thr Val Ser Ile Glu Val Lys Glu Val Val Lys Pro Leu Leu 
                325                 330                 335     


Val Ser Thr Leu Gly Glu Lys Ser Gly Lys Gly Leu Lys Thr Cys Lys 
            340                 345                 350         


Ser Pro Gly Arg Lys Ser Lys Glu Ser Ser Pro Lys Gly Arg Ser Ser 
        355                 360                 365             


Ser Ala Ser Ser Pro Pro Lys Lys Glu His His His His His His His 
    370                 375                 380                 


Ser Glu Ser Pro Lys Ala Pro Val Pro Leu Leu Pro Pro Leu Pro Pro 
385                 390                 395                 400 


Pro Pro Pro Glu Pro Glu Ser Ser Glu Asp Pro Thr Ser Pro Pro Glu 
                405                 410                 415     


Pro Gln Asp Leu Ser Ser Ser Val Cys Lys Glu Glu Lys Met Pro Arg 
            420                 425                 430         


Gly Gly Ser Leu Glu Ser Asp Gly Cys Pro Lys Glu Pro Ala Lys Thr 
        435                 440                 445             


Gln Pro Ala Val Ala Thr Ala Ala Thr Ala Ala Glu Lys Tyr Lys His 
    450                 455                 460                 


Arg Gly Glu Gly Glu Arg Lys Asp Ile Val Ser Ser Ser Met Pro Arg 
465                 470                 475                 480 


Pro Asn Arg Glu Glu Pro Val Asp Ser Arg Thr Pro Val Thr Glu Arg 
                485                 490                 495     


Val Ser 
        


<210>  22
<211>  466
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  promoter

<400>  22
ctgcagaggg ccctgcgtat gagtgcaagt gggttttagg accaggatga ggcggggtgg       60

gggtgcctac ctgacgaccg accccgaccc actggacaag cacccaaccc ccattcccca      120

aattgcgcat cccctatcag agagggggag gggaaacagg atgcggcgag gcgcgtgcgc      180

actgccagct tcagcaccgc ggacagtgcc ttcgcccccg cctggcggcg cgcgccaccg      240

ccgcctcagc actgaaggcg cgctgacgtc actcgccggt cccccgcaaa ctccccttcc      300

cggccacctt ggtcgcgtcc gcgccgccgc cggcccagcc ggaccgcacc acgcgaggcg      360

cgagataggg gggcacgggc gcgaccatct gcgctgcggc gccggcgact cagcgctgcc      420

tcagtctgcg gtgggcagcg gaggagtcgt gtcgtgcctg agagcg                     466


<210>  23
<211>  2728
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression vector


<220>
<221>  repeat_region
<222>  (1)..(130)
<223>  5'ITR

<220>
<221>  promoter
<222>  (213)..(678)
<223>  hSynapsin

<220>
<221>  misc_feature
<222>  (690)..(695)
<223>  Kozak

<220>
<221>  misc_feature
<222>  (696)..(2195)
<223>  human MECP2 alpha

<220>
<221>  polyA_signal
<222>  (2303)..(2534)
<223>  SV40 late polyadenylation

<220>
<221>  repeat_region
<222>  (2599)..(2728)
<223>  3'ITR

<400>  23
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct      180

aggaagatcc tctagaacta tagctagcat gcctgcagag ggccctgcgt atgagtgcaa      240

gtgggtttta ggaccaggat gaggcggggt gggggtgcct acctgacgac cgaccccgac      300

ccactggaca agcacccaac ccccattccc caaattgcgc atcccctatc agagaggggg      360

aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc gcggacagtg      420

ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg      480

tcactcgccg gtcccccgca aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc      540

gccggcccag ccggaccgca ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat      600

ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc      660

gtgtcgtgcc tgagagcgca gtcgaattcg ccaccatggc tgctgcagct gctgctgcac      720

cttctggtgg tggcggaggc ggagaggaag agagactgga agagaagtcc gaggaccagg      780

atctgcaggg cctgaaggac aagcccctga agttcaagaa agtgaagaag gataagaaag      840

aggaaaaaga gggcaagcac gagcccgtgc agccatctgc tcaccattct gccgaacctg      900

ccgaagccgg aaaggccgaa acatctgaag gctctggaag cgcccctgcc gtgcctgaag      960

cttctgcttc tcctaagcag cggcggagca tcatcagaga cagaggccct atgtacgacg     1020

accccacact gcctgaaggc tggaccagaa agctgaagca gagaaagagc ggcagaagcg     1080

ccgggaagta cgacgtgtac ctgatcaacc ctcagggcaa agccttccgg tccaaggtgg     1140

aactgatcgc ctacttcgag aaagtgggcg acacctctct ggaccccaac gacttcgatt     1200

tcaccgtgac aggcagaggc agccccagca gaagagagca gaagcctcct aagaagccta     1260

agagccccaa ggctcctggc accggaagag gtagaggtag acctaaaggc agcggcacca     1320

caagacctaa ggccgctact tctgagggcg tgcaagtgaa gagagtgctg gaaaagagcc     1380

ccggcaagct gctggtcaag atgccctttc agacaagccc tggcggcaaa gcagaaggcg     1440

gaggggctac aacaagcacc caagtgatgg tcatcaagag gcccggcaga aagcggaagg     1500

ccgaagctga tcctcaggct atccccaaga agagaggccg gaagcctgga tctgtggttg     1560

ctgctgccgc cgcagaggcc aagaaaaagg ccgtgaaaga gtccagcatc cgcagcgtgc     1620

aagagacagt gctgcccatc aagaagcgca agacccggga aaccgtgtcc atcgaagtga     1680

aagaggtggt caagcctctg ctggtgtcta ccctgggcga gaagtctggc aagggactga     1740

aaacctgcaa gtcccctggc agaaagagca aagagtctag ccctaagggc agaagcagca     1800

gcgctagctc cccacctaag aaagaacacc accatcacca ccaccatagc gagtccccaa     1860

aggctccagt tcctctgctt cctccactgc ctccacctcc acctgagcct gagtctagcg     1920

aggatcctac aagccctcct gagccacagg atctgagcag ctccgtgtgc aaagaagaga     1980

agatgcccag aggcggctcc ctggaatctg acggctgtcc taaagagccc gccaagacac     2040

agcctgccgt tgccacagct gcaacagctg ccgagaagta caagcacaga ggcgagggcg     2100

agcgcaagga tatcgtgtct agcagcatgc ccagacctaa ccgcgaggaa cccgtggata     2160

gcagaacccc tgtgaccgag agggtgtcct gatgactcga cggcgcgccc atggcgatat     2220

ccaattggag ctcgcggccg cctcgactta ccggtttgat atcttctaga aggatccaac     2280

gcgtaggtac cggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca     2340

aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc     2400

tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt     2460

tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa     2520

atgtggtaaa atcgataagg atcttcctag agcatggcta cgtagataag tagcatggcg     2580

ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg     2640

ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg     2700

cggcctcagt gagcgagcga gcgcgcag                                        2728


<210>  24
<211>  3948
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression Vector


<220>
<221>  repeat_region
<222>  (1)..(130)
<223>  5'ITR

<220>
<221>  repeat_region
<222>  (198)..(579)
<223>  CMV IE promoter

<220>
<221>  promoter
<222>  (582)..(863)
<223>  CB promoter

<220>
<221>  TATA_signal
<222>  (836)..(839)
<223>  TATA

<220>
<221>  Intron
<222>  (957)..(1929)
<223>  chicken beta-actin intron

<220>
<221>  misc_feature
<222>  (1993)..(3486)
<223>  MECP2 e1

<220>
<221>  misc_feature
<222>  (3490)..(3537)
<223>  SpA

<220>
<221>  polyA_signal
<222>  (3604)..(3730)
<223>  Rabbit globin poly A

<220>
<221>  repeat_region
<222>  (3819)..(3948)
<223>  3'ITR

<400>  24
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg      180

atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat      240

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      300

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      360

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      420

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt      480

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc      540

tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac      600

gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat      660

tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg      720

ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca      780

gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa      840

aaagcgaagc gcgcggcggg cggggagtcg ctgcgcgctg ccttcgcccc gtgccccgct      900

ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga      960

gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg     1020

tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg     1080

ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg     1140

cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg     1200

tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga     1260

acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg     1320

tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg     1380

gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc     1440

aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc     1500

gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt     1560

tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga     1620

aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg     1680

gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc     1740

ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg     1800

cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg     1860

ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt     1920

ttggcaaaga attcctgtac ggaagtgtta cttctgctct aaaagctgcg gaattgtacc     1980

cgcgggccca ccatggccgc cgccgccgcc gccgcgccga gcggaggagg aggaggaggc     2040

gaggaggaga gactggaaga aaagtcagaa gaccaggacc tccagggcct caaggacaaa     2100

cccctcaagt ttaaaaaggt gaagaaagat aagaaagaag agaaagaggg caagcatgag     2160

cccgtgcagc catcagccca ccactctgct gagcccgcag aggcaggcaa agcagagaca     2220

tcagaagggt caggctccgc cccggctgtg ccggaagctt ctgcctcccc caaacagcgg     2280

cgctccatca tccgtgaccg gggacccatg tatgatgacc ccaccctgcc tgaaggctgg     2340

acacggaagc ttaagcaaag gaaatctggc cgctctgctg ggaagtatga tgtgtatttg     2400

atcaatcccc agggaaaagc ctttcgctct aaagtggagt tgattgcgta cttcgaaaag     2460

gtaggcgaca catccctgga ccctaatgat tttgacttca cggtaactgg gagagggagc     2520

ccctcccggc gagagcagaa accacctaag aagcccaaat ctcccaaagc tccaggaact     2580

ggcagaggcc ggggacgccc caaagggagc ggcaccacga gacccaaggc ggccacgtca     2640

gagggtgtgc aggtgaaaag ggtcctggag aaaagtcctg ggaagctcct tgtcaagatg     2700

ccttttcaaa cttcgccagg gggcaaggct gaggggggtg gggccaccac atccacccag     2760

gtcatggtga tcaaacgccc cggcaggaag cgaaaagctg aggccgaccc tcaggccatt     2820

cccaagaaac ggggccgaaa gccggggagt gtggtggcag ccgctgccgc cgaggccaaa     2880

aagaaagccg tgaaggagtc ttctatccga tctgtgcagg agaccgtact ccccatcaag     2940

aagcgcaaga cccgggagac ggtcagcatc gaggtcaagg aagtggtgaa gcccctgctg     3000

gtgtccaccc tcggtgagaa gagcgggaaa ggactgaaga cctgtaagag ccctgggcgg     3060

aaaagcaagg agagcagccc caaggggcgc agcagcagcg cctcctcacc ccccaagaag     3120

gagcaccacc accatcacca ccactcagag tccccaaagg cccccgtgcc actgctccca     3180

cccctgcccc cacctccacc tgagcccgag agctccgagg accccaccag cccccctgag     3240

ccccaggact tgagcagcag cgtctgcaaa gaggagaaga tgcccagagg aggctcactg     3300

gagagcgacg gctgccccaa ggagccagct aagactcagc ccgcggttgc caccgccgcc     3360

acggccgcag aaaagtacaa acaccgaggg gagggagagc gcaaagacat tgtttcatcc     3420

tccatgccaa ggccaaacag agaggagcct gtggacagcc ggacgcccgt gaccgagaga     3480

gttagctaga ataaagagct cagatgcatc gatcagagtg tgttggtttt ttgtgtgacg     3540

cgtggtacct ctagagtcga cccgggcggc ctcgaggacg gggtgaacta cgcctgagga     3600

tccgatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg     3660

acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt     3720

ctctcactcg gaagcaattc gttgatctga atttcgacca cccataatac ccattaccct     3780

ggtagataag tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt     3840

ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg     3900

acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcag                  3948


