                         SEQUENCE LISTING

<110>  The Trustees of the Univeristy of Pennsylvania
       Powell, Daniel J
       Minutolo, Nicholas
       Farwell, Michael
 
<120>  A DOTA-Binding Chimeric Antigen Receptor for Cellular Therapy

<130>  046483-7240WO1(02302)

<150>  62/837,569
<151>  2019-04-23

<160>  40    

<170>  PatentIn version 3.5

<210>  1
<211>  732
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Humanized DOTA-binding scFV (hC825)

<400>  1
catgttcagt tagtcgagtc cggtggagga ctggtgcaac caggaggttc tctgagattg       60

tcctgtgccg cctctggctt tagtctgaca gattacggag tgcactgggt taggcaggcc      120

cctgggaaag gtttggaatg gttaggagtt atttggtccg gcggagggac agcttacaat      180

accgcactga tttcaagatt cactatctca agggataaca gcaagaacac attgtatctg      240

caaatgaata gcttgagagc cgaggacacc gctgtctatt actgtgctag aaggggtagt      300

tatccctaca actatttcga cgcatggggc tgcggaactc tggtcacagt gtcttccggt      360

ggaggaggga gcggtggagg agggagtggt ggaggagggt ctcaggcagt ggtgacacaa      420

gaacccagtt tgaccgtctc tccaggtggc actgtgacat taacctgtgg gtcaagcact      480

ggtgctgtta cagcaagcaa ctacgcaaat tgggtgcagc agaaacctgg gcagtgtcct      540

cggggcctga ttggcgggca taataacaga cctcctgggg tgccagctcg gttcagcggc      600

agcctgctgg gagggaaggc agctctgacc ctgctgggag cacagcctga ggacgaagca      660

gagtactatt gcgccctgtg gtactctgat cactgggtca tcggtggtgg aaccaagctg      720

actgtcttgg gc                                                          732


<210>  2
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized DOTA-binding scFV (hC825)

<400>  2

His Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Leu Thr Asp Tyr 
            20                  25                  30          


Gly Val His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Ser Gly Gly Gly Thr Ala Tyr Asn Thr Ala Leu Ile 
    50                  55                  60                  


Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Arg Gly Ser Tyr Pro Tyr Asn Tyr Phe Asp Ala Trp Gly Cys Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gln Ala Val Val Thr Gln Glu Pro Ser Leu 
    130                 135                 140                 


Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Gly Ser Ser Thr 
145                 150                 155                 160 


Gly Ala Val Thr Ala Ser Asn Tyr Ala Asn Trp Val Gln Gln Lys Pro 
                165                 170                 175     


Gly Gln Cys Pro Arg Gly Leu Ile Gly Gly His Asn Asn Arg Pro Pro 
            180                 185                 190         


Gly Val Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly Lys Ala Ala 
        195                 200                 205             


Leu Thr Leu Leu Gly Ala Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys 
    210                 215                 220                 


Ala Leu Trp Tyr Ser Asp His Trp Val Ile Gly Gly Gly Thr Lys Leu 
225                 230                 235                 240 


Thr Val Leu Gly 
                


<210>  3
<211>  732
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Humanized DOTA-binding scFV (hC825/G54C)

<400>  3
catgttcagt tagtcgagtc cggtggagga ctggtgcaac caggaggttc tctgagattg       60

tcctgtgccg cctctggctt tagtctgaca gattacggag tgcactgggt taggcaggcc      120

cctgggaaag gtttggaatg gttaggagtt atttggtcct gcggagggac agcttacaat      180

accgcactga tttcaagatt cactatctca agggataaca gcaagaacac attgtatctg      240

caaatgaata gcttgagagc cgaggacacc gctgtctatt actgtgctag aaggggtagt      300

tatccctaca actatttcga cgcatggggc tgcggaactc tggtcacagt gtcttccggt      360

ggaggaggga gcggtggagg agggagtggt ggaggagggt ctcaggcagt ggtgacacaa      420

gaacccagtt tgaccgtctc tccaggtggc actgtgacat taacctgtgg gtcaagcact      480

ggtgctgtta cagcaagcaa ctacgcaaat tgggtgcagc agaaacctgg gcagtgtcct      540

cggggcctga ttggcgggca taataacaga cctcctgggg tgccagctcg gttcagcggc      600

agcctgctgg gagggaaggc agctctgacc ctgctgggag cacagcctga ggacgaagca      660

gagtactatt gcgccctgtg gtactctgat cactgggtca tcggtggtgg aaccaagctg      720

actgtcttgg gc                                                          732


<210>  4
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized DOTA-binding scFV (hC825/G54C)

<400>  4

His Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Leu Thr Asp Tyr 
            20                  25                  30          


Gly Val His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Ser Cys Gly Gly Thr Ala Tyr Asn Thr Ala Leu Ile 
    50                  55                  60                  


Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Arg Gly Ser Tyr Pro Tyr Asn Tyr Phe Asp Ala Trp Gly Cys Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gln Ala Val Val Thr Gln Glu Pro Ser Leu 
    130                 135                 140                 


Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Gly Ser Ser Thr 
145                 150                 155                 160 


Gly Ala Val Thr Ala Ser Asn Tyr Ala Asn Trp Val Gln Gln Lys Pro 
                165                 170                 175     


Gly Gln Cys Pro Arg Gly Leu Ile Gly Gly His Asn Asn Arg Pro Pro 
            180                 185                 190         


Gly Val Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly Lys Ala Ala 
        195                 200                 205             


Leu Thr Leu Leu Gly Ala Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys 
    210                 215                 220                 


Ala Leu Trp Tyr Ser Asp His Trp Val Ile Gly Gly Gly Thr Lys Leu 
225                 230                 235                 240 


Thr Val Leu Gly 
                


<210>  5
<211>  1491
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DOTA CAR hC825-28z

<400>  5
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgggatccc atgttcagtt agtcgagtcc ggtggaggac tggtgcaacc aggaggttct      120

ctgagattgt cctgtgccgc ctctggcttt agtctgacag attacggagt gcactgggtt      180

aggcaggccc ctgggaaagg tttggaatgg ttaggagtta tttggtccgg cggagggaca      240

gcttacaata ccgcactgat ttcaagattc actatctcaa gggataacag caagaacaca      300

ttgtatctgc aaatgaatag cttgagagcc gaggacaccg ctgtctatta ctgtgctaga      360

aggggtagtt atccctacaa ctatttcgac gcatggggct gcggaactct ggtcacagtg      420

tcttccggtg gaggagggag cggtggagga gggagtggtg gaggagggtc tcaggcagtg      480

gtgacacaag aacccagttt gaccgtctct ccaggtggca ctgtgacatt aacctgtggg      540

tcaagcactg gtgctgttac agcaagcaac tacgcaaatt gggtgcagca gaaacctggg      600

cagtgtcctc ggggcctgat tggcgggcat aataacagac ctcctggggt gccagctcgg      660

ttcagcggca gcctgctggg agggaaggca gctctgaccc tgctgggagc acagcctgag      720

gacgaagcag agtactattg cgccctgtgg tactctgatc actgggtcat cggtggtgga      780

accaagctga ctgtcttggg cgctagcacc acgacgccag cgccgcgacc accaacaccg      840

gcgcccacca tcgcgtcgca gcccctgtcc ctgcgcccag aggcgtgccg gccagcggcg      900

gggggcgcag tgcacacgag ggggctggac ttcgcctgtg atttttgggt gctggtggtg      960

gttggtggag tcctggcttg ctatagcttg ctagtaacag tggcctttat tattttctgg     1020

gtgaggagta agaggagcag gctcctgcac agtgactaca tgaacatgac tccccgccgc     1080

cccgggccca cccgcaagca ttaccagccc tatgccccac cacgcgactt cgcagcctat     1140

cgctccatcg atagagtgaa gttcagcagg agcgcagacg cccccgcgta ccagcagggc     1200

cagaaccagc tctataacga gctcaatcta ggacgaagag aggagtacga tgttttggac     1260

aagagacgtg gccgggaccc tgagatgggg ggaaagccga gaaggaagaa ccctcaggaa     1320

ggcctgtaca atgaactgca gaaagataag atggcggagg cctacagtga gattgggatg     1380

aaaggcgagc gccggagggg caaggggcac gatggccttt accagggtct cagtacagcc     1440

accaaggaca cctacgacgc ccttcacatg caggccctgc cccctcgcta a              1491


<210>  6
<211>  496
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DOTA CAR hC825-28z

<400>  6

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gly Ser His Val Gln Leu Val Glu Ser Gly Gly 
            20                  25                  30          


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
        35                  40                  45              


Gly Phe Ser Leu Thr Asp Tyr Gly Val His Trp Val Arg Gln Ala Pro 
    50                  55                  60                  


Gly Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Ser Gly Gly Gly Thr 
65                  70                  75                  80  


Ala Tyr Asn Thr Ala Leu Ile Ser Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Arg Gly Ser Tyr Pro Tyr Asn Tyr 
        115                 120                 125             


Phe Asp Ala Trp Gly Cys Gly Thr Leu Val Thr Val Ser Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val 
145                 150                 155                 160 


Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr 
                165                 170                 175     


Leu Thr Cys Gly Ser Ser Thr Gly Ala Val Thr Ala Ser Asn Tyr Ala 
            180                 185                 190         


Asn Trp Val Gln Gln Lys Pro Gly Gln Cys Pro Arg Gly Leu Ile Gly 
        195                 200                 205             


Gly His Asn Asn Arg Pro Pro Gly Val Pro Ala Arg Phe Ser Gly Ser 
    210                 215                 220                 


Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Leu Gly Ala Gln Pro Glu 
225                 230                 235                 240 


Asp Glu Ala Glu Tyr Tyr Cys Ala Leu Trp Tyr Ser Asp His Trp Val 
                245                 250                 255     


Ile Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ala Ser Thr Thr Thr 
            260                 265                 270         


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
        275                 280                 285             


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
    290                 295                 300                 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Phe Trp Val Leu Val Val 
305                 310                 315                 320 


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
                325                 330                 335     


Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp 
            340                 345                 350         


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
        355                 360                 365             


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Ile Asp 
    370                 375                 380                 


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
385                 390                 395                 400 


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
                405                 410                 415     


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
            420                 425                 430         


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
        435                 440                 445             


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
    450                 455                 460                 


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
465                 470                 475                 480 


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490                 495     


<210>  7
<211>  1479
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DOTA CAR hC825-BBz

<400>  7
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgggatccc atgttcagtt agtcgagtcc ggtggaggac tggtgcaacc aggaggttct      120

ctgagattgt cctgtgccgc ctctggcttt agtctgacag attacggagt gcactgggtt      180

aggcaggccc ctgggaaagg tttggaatgg ttaggagtta tttggtccgg cggagggaca      240

gcttacaata ccgcactgat ttcaagattc actatctcaa gggataacag caagaacaca      300

ttgtatctgc aaatgaatag cttgagagcc gaggacaccg ctgtctatta ctgtgctaga      360

aggggtagtt atccctacaa ctatttcgac gcatggggct gcggaactct ggtcacagtg      420

tcttccggtg gaggagggag cggtggagga gggagtggtg gaggagggtc tcaggcagtg      480

gtgacacaag aacccagttt gaccgtctct ccaggtggca ctgtgacatt aacctgtggg      540

tcaagcactg gtgctgttac agcaagcaac tacgcaaatt gggtgcagca gaaacctggg      600

cagtgtcctc ggggcctgat tggcgggcat aataacagac ctcctggggt gccagctcgg      660

ttcagcggca gcctgctggg agggaaggca gctctgaccc tgctgggagc acagcctgag      720

gacgaagcag agtactattg cgccctgtgg tactctgatc actgggtcat cggtggtgga      780

accaagctga ctgtcttggg cgctagcacc acgacgccag cgccgcgacc accaacaccg      840

gcgcccacca tcgcgtcgca gcccctgtcc ctgcgcccag aggcgtgccg gccagcggcg      900

gggggcgcag tgcacacgag ggggctggac ttcgcctgtg atatctacat ctgggcgccc      960

ttggccggga cttgtggggt ccttctcctg tcactggtta tcacccttta ctgcaaacgg     1020

ggcagaaaga aactcctgta tatattcaaa caaccattta tgagaccagt acaaactact     1080

caagaggaag atggctgtag ctgccgattt ccagaagaag aagaaggagg atgtgaactg     1140

agagtgaagt tcagcaggag cgcagacgcc cccgcgtaca agcagggcca gaaccagctc     1200

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc     1260

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat     1320

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc     1380

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc     1440

tacgacgccc ttcacatgca ggccctgccc cctcgctaa                            1479


<210>  8
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DOTA CAR hC825-BBz

<400>  8

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gly Ser His Val Gln Leu Val Glu Ser Gly Gly 
            20                  25                  30          


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
        35                  40                  45              


Gly Phe Ser Leu Thr Asp Tyr Gly Val His Trp Val Arg Gln Ala Pro 
    50                  55                  60                  


Gly Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Ser Gly Gly Gly Thr 
65                  70                  75                  80  


Ala Tyr Asn Thr Ala Leu Ile Ser Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Arg Gly Ser Tyr Pro Tyr Asn Tyr 
        115                 120                 125             


Phe Asp Ala Trp Gly Cys Gly Thr Leu Val Thr Val Ser Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val 
145                 150                 155                 160 


Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr 
                165                 170                 175     


Leu Thr Cys Gly Ser Ser Thr Gly Ala Val Thr Ala Ser Asn Tyr Ala 
            180                 185                 190         


Asn Trp Val Gln Gln Lys Pro Gly Gln Cys Pro Arg Gly Leu Ile Gly 
        195                 200                 205             


Gly His Asn Asn Arg Pro Pro Gly Val Pro Ala Arg Phe Ser Gly Ser 
    210                 215                 220                 


Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Leu Gly Ala Gln Pro Glu 
225                 230                 235                 240 


Asp Glu Ala Glu Tyr Tyr Cys Ala Leu Trp Tyr Ser Asp His Trp Val 
                245                 250                 255     


Ile Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ala Ser Thr Thr Thr 
            260                 265                 270         


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
        275                 280                 285             


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
    290                 295                 300                 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro 
305                 310                 315                 320 


Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu 
                325                 330                 335     


Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro 
            340                 345                 350         


Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys 
        355                 360                 365             


Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe 
    370                 375                 380                 


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu 
385                 390                 395                 400 


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
                405                 410                 415     


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
            420                 425                 430         


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
        435                 440                 445             


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
    450                 455                 460                 


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
465                 470                 475                 480 


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490         


<210>  9
<211>  1041
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DOTA CAR hC825-dz

<400>  9
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgggatccc atgttcagtt agtcgagtcc ggtggaggac tggtgcaacc aggaggttct      120

ctgagattgt cctgtgccgc ctctggcttt agtctgacag attacggagt gcactgggtt      180

aggcaggccc ctgggaaagg tttggaatgg ttaggagtta tttggtccgg cggagggaca      240

gcttacaata ccgcactgat ttcaagattc actatctcaa gggataacag caagaacaca      300

ttgtatctgc aaatgaatag cttgagagcc gaggacaccg ctgtctatta ctgtgctaga      360

aggggtagtt atccctacaa ctatttcgac gcatggggct gcggaactct ggtcacagtg      420

tcttccggtg gaggagggag cggtggagga gggagtggtg gaggagggtc tcaggcagtg      480

gtgacacaag aacccagttt gaccgtctct ccaggtggca ctgtgacatt aacctgtggg      540

tcaagcactg gtgctgttac agcaagcaac tacgcaaatt gggtgcagca gaaacctggg      600

cagtgtcctc ggggcctgat tggcgggcat aataacagac ctcctggggt gccagctcgg      660

ttcagcggca gcctgctggg agggaaggca gctctgaccc tgctgggagc acagcctgag      720

gacgaagcag agtactattg cgccctgtgg tactctgatc actgggtcat cggtggtgga      780

accaagctga ctgtcttggg cgctagcacc acgacgccag cgccgcgacc accaacaccg      840

gcgcccacca tcgcgtcgca gcccctgtcc ctgcgcccag aggcgtgccg gccagcggcg      900

gggggcgcag tgcacacgag ggggctggac ttcgcctgtg atttttgggt gctggtggtg      960

gttggtggag tcctggcttg ctatagcttg ctagtaacag tggcctttat tattttctgg     1020

gtgaggagta agaggagcta a                                               1041


<210>  10
<211>  346
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DOTA CAR hC825-dz

<400>  10

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gly Ser His Val Gln Leu Val Glu Ser Gly Gly 
            20                  25                  30          


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
        35                  40                  45              


Gly Phe Ser Leu Thr Asp Tyr Gly Val His Trp Val Arg Gln Ala Pro 
    50                  55                  60                  


Gly Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Ser Gly Gly Gly Thr 
65                  70                  75                  80  


Ala Tyr Asn Thr Ala Leu Ile Ser Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Arg Gly Ser Tyr Pro Tyr Asn Tyr 
        115                 120                 125             


Phe Asp Ala Trp Gly Cys Gly Thr Leu Val Thr Val Ser Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val 
145                 150                 155                 160 


Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr 
                165                 170                 175     


Leu Thr Cys Gly Ser Ser Thr Gly Ala Val Thr Ala Ser Asn Tyr Ala 
            180                 185                 190         


Asn Trp Val Gln Gln Lys Pro Gly Gln Cys Pro Arg Gly Leu Ile Gly 
        195                 200                 205             


Gly His Asn Asn Arg Pro Pro Gly Val Pro Ala Arg Phe Ser Gly Ser 
    210                 215                 220                 


Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Leu Gly Ala Gln Pro Glu 
225                 230                 235                 240 


Asp Glu Ala Glu Tyr Tyr Cys Ala Leu Trp Tyr Ser Asp His Trp Val 
                245                 250                 255     


Ile Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ala Ser Thr Thr Thr 
            260                 265                 270         


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
        275                 280                 285             


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
    290                 295                 300                 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Phe Trp Val Leu Val Val 
305                 310                 315                 320 


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
                325                 330                 335     


Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
            340                 345     


<210>  11
<211>  1491
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Covalent (G54C) DOTA CAR hC825G54C-28z

<400>  11
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgggatccc atgttcagtt agtcgagtcc ggtggaggac tggtgcaacc aggaggttct      120

ctgagattgt cctgtgccgc ctctggcttt agtctgacag attacggagt gcactgggtt      180

aggcaggccc ctgggaaagg tttggaatgg ttaggagtta tttggtcctg cggagggaca      240

gcttacaata ccgcactgat ttcaagattc actatctcaa gggataacag caagaacaca      300

ttgtatctgc aaatgaatag cttgagagcc gaggacaccg ctgtctatta ctgtgctaga      360

aggggtagtt atccctacaa ctatttcgac gcatggggct gcggaactct ggtcacagtg      420

tcttccggtg gaggagggag cggtggagga gggagtggtg gaggagggtc tcaggcagtg      480

gtgacacaag aacccagttt gaccgtctct ccaggtggca ctgtgacatt aacctgtggg      540

tcaagcactg gtgctgttac agcaagcaac tacgcaaatt gggtgcagca gaaacctggg      600

cagtgtcctc ggggcctgat tggcgggcat aataacagac ctcctggggt gccagctcgg      660

ttcagcggca gcctgctggg agggaaggca gctctgaccc tgctgggagc acagcctgag      720

gacgaagcag agtactattg cgccctgtgg tactctgatc actgggtcat cggtggtgga      780

accaagctga ctgtcttggg cgctagcacc acgacgccag cgccgcgacc accaacaccg      840

gcgcccacca tcgcgtcgca gcccctgtcc ctgcgcccag aggcgtgccg gccagcggcg      900

gggggcgcag tgcacacgag ggggctggac ttcgcctgtg atttttgggt gctggtggtg      960

gttggtggag tcctggcttg ctatagcttg ctagtaacag tggcctttat tattttctgg     1020

gtgaggagta agaggagcag gctcctgcac agtgactaca tgaacatgac tccccgccgc     1080

cccgggccca cccgcaagca ttaccagccc tatgccccac cacgcgactt cgcagcctat     1140

cgctccatcg atagagtgaa gttcagcagg agcgcagacg cccccgcgta ccagcagggc     1200

cagaaccagc tctataacga gctcaatcta ggacgaagag aggagtacga tgttttggac     1260

aagagacgtg gccgggaccc tgagatgggg ggaaagccga gaaggaagaa ccctcaggaa     1320

ggcctgtaca atgaactgca gaaagataag atggcggagg cctacagtga gattgggatg     1380

aaaggcgagc gccggagggg caaggggcac gatggccttt accagggtct cagtacagcc     1440

accaaggaca cctacgacgc ccttcacatg caggccctgc cccctcgcta a              1491


<210>  12
<211>  496
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Covalent (G54C) DOTA CAR hC825G54C-28z

<400>  12

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gly Ser His Val Gln Leu Val Glu Ser Gly Gly 
            20                  25                  30          


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
        35                  40                  45              


Gly Phe Ser Leu Thr Asp Tyr Gly Val His Trp Val Arg Gln Ala Pro 
    50                  55                  60                  


Gly Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Ser Cys Gly Gly Thr 
65                  70                  75                  80  


Ala Tyr Asn Thr Ala Leu Ile Ser Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Arg Gly Ser Tyr Pro Tyr Asn Tyr 
        115                 120                 125             


Phe Asp Ala Trp Gly Cys Gly Thr Leu Val Thr Val Ser Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val 
145                 150                 155                 160 


Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr 
                165                 170                 175     


Leu Thr Cys Gly Ser Ser Thr Gly Ala Val Thr Ala Ser Asn Tyr Ala 
            180                 185                 190         


Asn Trp Val Gln Gln Lys Pro Gly Gln Cys Pro Arg Gly Leu Ile Gly 
        195                 200                 205             


Gly His Asn Asn Arg Pro Pro Gly Val Pro Ala Arg Phe Ser Gly Ser 
    210                 215                 220                 


Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Leu Gly Ala Gln Pro Glu 
225                 230                 235                 240 


Asp Glu Ala Glu Tyr Tyr Cys Ala Leu Trp Tyr Ser Asp His Trp Val 
                245                 250                 255     


Ile Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ala Ser Thr Thr Thr 
            260                 265                 270         


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
        275                 280                 285             


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
    290                 295                 300                 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Phe Trp Val Leu Val Val 
305                 310                 315                 320 


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
                325                 330                 335     


Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp 
            340                 345                 350         


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
        355                 360                 365             


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Ile Asp 
    370                 375                 380                 


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
385                 390                 395                 400 


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
                405                 410                 415     


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
            420                 425                 430         


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
        435                 440                 445             


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
    450                 455                 460                 


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
465                 470                 475                 480 


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490                 495     


<210>  13
<211>  1479
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Covalent (G54C) DOTA CAR hC825G54C-BBz

<400>  13
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgggatccc atgttcagtt agtcgagtcc ggtggaggac tggtgcaacc aggaggttct      120

ctgagattgt cctgtgccgc ctctggcttt agtctgacag attacggagt gcactgggtt      180

aggcaggccc ctgggaaagg tttggaatgg ttaggagtta tttggtcctg cggagggaca      240

gcttacaata ccgcactgat ttcaagattc actatctcaa gggataacag caagaacaca      300

ttgtatctgc aaatgaatag cttgagagcc gaggacaccg ctgtctatta ctgtgctaga      360

aggggtagtt atccctacaa ctatttcgac gcatggggct gcggaactct ggtcacagtg      420

tcttccggtg gaggagggag cggtggagga gggagtggtg gaggagggtc tcaggcagtg      480

gtgacacaag aacccagttt gaccgtctct ccaggtggca ctgtgacatt aacctgtggg      540

tcaagcactg gtgctgttac agcaagcaac tacgcaaatt gggtgcagca gaaacctggg      600

cagtgtcctc ggggcctgat tggcgggcat aataacagac ctcctggggt gccagctcgg      660

ttcagcggca gcctgctggg agggaaggca gctctgaccc tgctgggagc acagcctgag      720

gacgaagcag agtactattg cgccctgtgg tactctgatc actgggtcat cggtggtgga      780

accaagctga ctgtcttggg cgctagcacc acgacgccag cgccgcgacc accaacaccg      840

gcgcccacca tcgcgtcgca gcccctgtcc ctgcgcccag aggcgtgccg gccagcggcg      900

gggggcgcag tgcacacgag ggggctggac ttcgcctgtg atatctacat ctgggcgccc      960

ttggccggga cttgtggggt ccttctcctg tcactggtta tcacccttta ctgcaaacgg     1020

ggcagaaaga aactcctgta tatattcaaa caaccattta tgagaccagt acaaactact     1080

caagaggaag atggctgtag ctgccgattt ccagaagaag aagaaggagg atgtgaactg     1140

agagtgaagt tcagcaggag cgcagacgcc cccgcgtaca agcagggcca gaaccagctc     1200

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc     1260

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat     1320

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc     1380

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc     1440

tacgacgccc ttcacatgca ggccctgccc cctcgctaa                            1479


<210>  14
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Covalent (G54C) DOTA CAR hC825G54C-BBz

<400>  14

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gly Ser His Val Gln Leu Val Glu Ser Gly Gly 
            20                  25                  30          


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
        35                  40                  45              


Gly Phe Ser Leu Thr Asp Tyr Gly Val His Trp Val Arg Gln Ala Pro 
    50                  55                  60                  


Gly Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Ser Cys Gly Gly Thr 
65                  70                  75                  80  


Ala Tyr Asn Thr Ala Leu Ile Ser Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Arg Gly Ser Tyr Pro Tyr Asn Tyr 
        115                 120                 125             


Phe Asp Ala Trp Gly Cys Gly Thr Leu Val Thr Val Ser Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val 
145                 150                 155                 160 


Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr 
                165                 170                 175     


Leu Thr Cys Gly Ser Ser Thr Gly Ala Val Thr Ala Ser Asn Tyr Ala 
            180                 185                 190         


Asn Trp Val Gln Gln Lys Pro Gly Gln Cys Pro Arg Gly Leu Ile Gly 
        195                 200                 205             


Gly His Asn Asn Arg Pro Pro Gly Val Pro Ala Arg Phe Ser Gly Ser 
    210                 215                 220                 


Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Leu Gly Ala Gln Pro Glu 
225                 230                 235                 240 


Asp Glu Ala Glu Tyr Tyr Cys Ala Leu Trp Tyr Ser Asp His Trp Val 
                245                 250                 255     


Ile Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ala Ser Thr Thr Thr 
            260                 265                 270         


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
        275                 280                 285             


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
    290                 295                 300                 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro 
305                 310                 315                 320 


Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu 
                325                 330                 335     


Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro 
            340                 345                 350         


Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys 
        355                 360                 365             


Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe 
    370                 375                 380                 


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu 
385                 390                 395                 400 


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
                405                 410                 415     


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
            420                 425                 430         


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
        435                 440                 445             


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
    450                 455                 460                 


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
465                 470                 475                 480 


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490         


<210>  15
<211>  1041
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Covalent (G54C) DOTA CAR hC825G54C-dz

<400>  15
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgggatccc atgttcagtt agtcgagtcc ggtggaggac tggtgcaacc aggaggttct      120

ctgagattgt cctgtgccgc ctctggcttt agtctgacag attacggagt gcactgggtt      180

aggcaggccc ctgggaaagg tttggaatgg ttaggagtta tttggtcctg cggagggaca      240

gcttacaata ccgcactgat ttcaagattc actatctcaa gggataacag caagaacaca      300

ttgtatctgc aaatgaatag cttgagagcc gaggacaccg ctgtctatta ctgtgctaga      360

aggggtagtt atccctacaa ctatttcgac gcatggggct gcggaactct ggtcacagtg      420

tcttccggtg gaggagggag cggtggagga gggagtggtg gaggagggtc tcaggcagtg      480

gtgacacaag aacccagttt gaccgtctct ccaggtggca ctgtgacatt aacctgtggg      540

tcaagcactg gtgctgttac agcaagcaac tacgcaaatt gggtgcagca gaaacctggg      600

cagtgtcctc ggggcctgat tggcgggcat aataacagac ctcctggggt gccagctcgg      660

ttcagcggca gcctgctggg agggaaggca gctctgaccc tgctgggagc acagcctgag      720

gacgaagcag agtactattg cgccctgtgg tactctgatc actgggtcat cggtggtgga      780

accaagctga ctgtcttggg cgctagcacc acgacgccag cgccgcgacc accaacaccg      840

gcgcccacca tcgcgtcgca gcccctgtcc ctgcgcccag aggcgtgccg gccagcggcg      900

gggggcgcag tgcacacgag ggggctggac ttcgcctgtg atttttgggt gctggtggtg      960

gttggtggag tcctggcttg ctatagcttg ctagtaacag tggcctttat tattttctgg     1020

gtgaggagta agaggagcta a                                               1041


<210>  16
<211>  346
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Covalent (G54C) DOTA CAR hC825G54C-dz

<400>  16

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gly Ser His Val Gln Leu Val Glu Ser Gly Gly 
            20                  25                  30          


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
        35                  40                  45              


Gly Phe Ser Leu Thr Asp Tyr Gly Val His Trp Val Arg Gln Ala Pro 
    50                  55                  60                  


Gly Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Ser Cys Gly Gly Thr 
65                  70                  75                  80  


Ala Tyr Asn Thr Ala Leu Ile Ser Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Arg Gly Ser Tyr Pro Tyr Asn Tyr 
        115                 120                 125             


Phe Asp Ala Trp Gly Cys Gly Thr Leu Val Thr Val Ser Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val 
145                 150                 155                 160 


Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr 
                165                 170                 175     


Leu Thr Cys Gly Ser Ser Thr Gly Ala Val Thr Ala Ser Asn Tyr Ala 
            180                 185                 190         


Asn Trp Val Gln Gln Lys Pro Gly Gln Cys Pro Arg Gly Leu Ile Gly 
        195                 200                 205             


Gly His Asn Asn Arg Pro Pro Gly Val Pro Ala Arg Phe Ser Gly Ser 
    210                 215                 220                 


Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Leu Gly Ala Gln Pro Glu 
225                 230                 235                 240 


Asp Glu Ala Glu Tyr Tyr Cys Ala Leu Trp Tyr Ser Asp His Trp Val 
                245                 250                 255     


Ile Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ala Ser Thr Thr Thr 
            260                 265                 270         


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
        275                 280                 285             


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
    290                 295                 300                 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Phe Trp Val Leu Val Val 
305                 310                 315                 320 


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
                325                 330                 335     


Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
            340                 345     


<210>  17
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8a hinge

<400>  17

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  18
<211>  135
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8a hinge

<400>  18
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg       60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      120

gacttcgcct gtgat                                                       135


<210>  19
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8a transmembrane domain

<400>  19

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  20
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8a transmembrane domain

<400>  20
atctacatct gggcgccctt ggccgggact tgtggggtcc ttctcctgtc actggttatc       60

accctttact gc                                                           72


<210>  21
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  41BB

<400>  21

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  22
<211>  126
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  41BB

<400>  22
aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa       60

actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt      120

gaactg                                                                 126


<210>  23
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3zeta

<400>  23

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  24
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD3zeta

<400>  24
agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat      180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc      240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc      300

tacgacgccc ttcacatgca ggccctgccc cctcgc                                336


<210>  25
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3zeta Q14K

<400>  25

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  26
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD3zeta Q14K

<400>  26
agagtgaagt tcagcaggag cgcagacgcc cccgcgtaca agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat      180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc      240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc      300

tacgacgccc ttcacatgca ggccctgccc cctcgctaa                             339


<210>  27
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain

<400>  27

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  28
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain

<400>  28
ttttgggtgc tggtggtggt tggtggagtc ctggcttgct atagcttgct agtaacagtg       60

gcctttatta ttttctgggt g                                                 81


<210>  29
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 intracellular domain

<400>  29

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  30
<211>  123
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD28 intracellular domain

<400>  30
aggagtaaga ggagcaggct cctgcacagt gactacatga acatgactcc ccgccgcccc       60

gggcccaccc gcaagcatta ccagccctat gccccaccac gcgacttcgc agcctatcgc      120

tcc                                                                    123


<210>  31
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LC CDR1

<400>  31

Gly Ser Ser Thr Gly Ala Val Thr Ala Ser Asn Tyr Ala Asn 
1               5                   10                  


<210>  32
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LC CDR2

<400>  32

Gly His Asn Asn Arg Pro Pro 
1               5           


<210>  33
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LC CDR3

<400>  33

Ala Leu Trp Tyr Ser Asp His Trp Val 
1               5                   


<210>  34
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HC CDR1

<400>  34

Gly Phe Ser Leu Thr Asp Tyr Gly Val His 
1               5                   10  


<210>  35
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HC CDR2 (hC825G54C)

<400>  35

Val Ile Trp Ser Cys Gly Gly Thr Ala Tyr Asn Thr Ala Leu Ile Ser 
1               5                   10                  15      


<210>  36
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HC CDR2

<400>  36

Val Ile Trp Ser Gly Gly Gly Thr Ala Tyr Asn Thr Ala Leu Ile Ser 
1               5                   10                  15      


<210>  37
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HC CDR3

<400>  37

Arg Gly Ser Tyr Pro Tyr Asn Tyr Phe Asp Ala 
1               5                   10      


<210>  38
<211>  9218
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pELNS-hC825-28Z

<400>  38
gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg       60

aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc      120

gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg      180

ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat      240

gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg      300

gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg      360

atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc      420

ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt      480

cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct      540

cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc      600

gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca      660

cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct      720

cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt      780

taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga      840

ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca      900

aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac      960

caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg     1020

taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag     1080

gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac     1140

cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt     1200

taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg     1260

agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc     1320

ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc     1380

gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc     1440

acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa     1500

acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt     1560

tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg     1620

ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag     1680

agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc     1740

acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc     1800

tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa     1860

ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg     1920

caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc     1980

aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga     2040

gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg     2100

aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga     2160

tattgtattt aagtgcctag ctcgatacaa taaacgggtc tctctggtta gaccagatct     2220

gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc     2280

cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc     2340

tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacctgaa     2400

agcgaaaggg aaaccagagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc     2460

aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa     2520

ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga     2580

aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc     2640

aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg     2700

tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc     2760

attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac     2820

caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca     2880

agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat     2940

tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga     3000

gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct     3060

tgggagcagc aggaagcact atgggcgcag cctcaatgac gctgacggta caggccagac     3120

aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac     3180

agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg     3240

tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca     3300

tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattg     3360

gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca     3420

ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt     3480

agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa     3540

attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc     3600

tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac     3660

cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga     3720

cagatccatt cgattagtga acggatctcg acggtatcga ttagactgta gcccaggaat     3780

atggcagcta gattgtacac atttagaagg aaaagttatc ttggtagcag ttcatgtagc     3840

cagtggatat atagaagcag aagtaattcc agcagagaca gggcaagaaa cagcatactt     3900

cctcttaaaa ttagcaggaa gatggccagt aaaaacagta catacagaca atggcagcaa     3960

tttcaccagt actacagtta aggccgcctg ttggtgggcg gggatcaagc aggaatttgg     4020

cattccctac aatccccaaa gtcaaggagt aatagaatct atgaataaag aattaaagaa     4080

aattatagga caggtaagag atcaggctga acatcttaag acagcagtac aaatggcagt     4140

attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat     4200

agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat     4260

tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggctgc atacgcgtcg     4320

tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg     4380

gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa     4440

agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt     4500

gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt     4560

gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga     4620

attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg     4680

tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc     4740

tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct     4800

gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc     4860

tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg     4920

gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg     4980

cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg     5040

cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca     5100

ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg     5160

aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt     5220

ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc     5280

gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg     5340

atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg     5400

taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag     5460

acagtggttc aaagtttttt tcttccattt caggtgtcgt gagctagacg actagtcgtc     5520

tagctctaga atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca     5580

cgccgccagg ccgggatccc atgttcagtt agtcgagtcc ggtggaggac tggtgcaacc     5640

aggaggttct ctgagattgt cctgtgccgc ctctggcttt agtctgacag attacggagt     5700

gcactgggtt aggcaggccc ctgggaaagg tttggaatgg ttaggagtta tttggtccgg     5760

cggagggaca gcttacaata ccgcactgat ttcaagattc actatctcaa gggataacag     5820

caagaacaca ttgtatctgc aaatgaatag cttgagagcc gaggacaccg ctgtctatta     5880

ctgtgctaga aggggtagtt atccctacaa ctatttcgac gcatggggct gcggaactct     5940

ggtcacagtg tcttccggtg gaggagggag cggtggagga gggagtggtg gaggagggtc     6000

tcaggcagtg gtgacacaag aacccagttt gaccgtctct ccaggtggca ctgtgacatt     6060

aacctgtggg tcaagcactg gtgctgttac agcaagcaac tacgcaaatt gggtgcagca     6120

gaaacctggg cagtgtcctc ggggcctgat tggcgggcat aataacagac ctcctggggt     6180

gccagctcgg ttcagcggca gcctgctggg agggaaggca gctctgaccc tgctgggagc     6240

acagcctgag gacgaagcag agtactattg cgccctgtgg tactctgatc actgggtcat     6300

cggtggtgga accaagctga ctgtcttggg cgctagcacc acgacgccag cgccgcgacc     6360

accaacaccg gcgcccacca tcgcgtcgca gcccctgtcc ctgcgcccag aggcgtgccg     6420

gccagcggcg gggggcgcag tgcacacgag ggggctggac ttcgcctgtg atttttgggt     6480

gctggtggtg gttggtggag tcctggcttg ctatagcttg ctagtaacag tggcctttat     6540

tattttctgg gtgaggagta agaggagcag gctcctgcac agtgactaca tgaacatgac     6600

tccccgccgc cccgggccca cccgcaagca ttaccagccc tatgccccac cacgcgactt     6660

cgcagcctat cgctccatcg atagagtgaa gttcagcagg agcgcagacg cccccgcgta     6720

ccagcagggc cagaaccagc tctataacga gctcaatcta ggacgaagag aggagtacga     6780

tgttttggac aagagacgtg gccgggaccc tgagatgggg ggaaagccga gaaggaagaa     6840

ccctcaggaa ggcctgtaca atgaactgca gaaagataag atggcggagg cctacagtga     6900

gattgggatg aaaggcgagc gccggagggg caaggggcac gatggccttt accagggtct     6960

cagtacagcc accaaggaca cctacgacgc ccttcacatg caggccctgc cccctcgcta     7020

agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta     7080

tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc     7140

ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga     7200

ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac     7260

ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc     7320

cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc     7380

tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg     7440

gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc     7500

ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc     7560

gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg     7620

aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac     7680

tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg     7740

ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc     7800

taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg     7860

tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg     7920

tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca     7980

aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa     8040

taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt     8100

ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa     8160

ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag     8220

aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag     8280

gcctagctag gcttttgcgt cgagacgtac ccaattcgcc ctatagtgag tcgtattacg     8340

cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac     8400

ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca     8460

ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcgac gcgccctgta     8520

gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca     8580

gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct     8640

ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc     8700

acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat     8760

agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc     8820

aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc     8880

cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta     8940

acaaaatatt aacgtttaca atttcccagg tggcactttt cggggaaatg tgcgcggaac     9000

ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc     9060

ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt     9120

cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct     9180

ggtgaaagta aaagatgctg aagatcagtt gggtgcac                             9218


<210>  39
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  portion of the CD28 intracellular domain

<400>  39
aggagtaaga ggagctaa                                                     18


<210>  40
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  portion of the CD28 intracellular domain

<400>  40

Arg Ser Lys Arg Ser 
1               5   


