                         SEQUENCE LISTING

<110>  BASF SE
 
<120>  Beta-Amylase Variants

<130>  190259

<150>  US62576930
<151>  2019-04-23

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  519
<212>  PRT
<213>  Clostridium Thermosulfurogenes

<400>  1

Ser Ile Ala Pro Asn Phe Lys Val Phe Val Met Gly Pro Leu Glu Lys 
1               5                   10                  15      


Val Thr Asp Phe Asn Ala Phe Lys Asp Gln Leu Ile Thr Leu Lys Asn 
            20                  25                  30          


Asn Gly Val Tyr Gly Ile Thr Thr Asp Ile Trp Trp Gly Tyr Val Glu 
        35                  40                  45              


Asn Ala Gly Glu Asn Gln Phe Asp Trp Ser Tyr Tyr Lys Thr Tyr Ala 
    50                  55                  60                  


Asp Thr Val Arg Ala Ala Gly Leu Lys Trp Val Pro Ile Met Ser Thr 
65                  70                  75                  80  


His Ala Cys Gly Gly Asn Val Gly Asp Thr Val Asn Ile Pro Ile Pro 
                85                  90                  95      


Ser Trp Val Trp Thr Lys Asp Thr Gln Asp Asn Met Gln Tyr Lys Asp 
            100                 105                 110         


Glu Ala Gly Asn Trp Asp Asn Glu Ala Val Ser Pro Trp Tyr Ser Gly 
        115                 120                 125             


Leu Thr Gln Leu Tyr Asn Glu Phe Tyr Ser Ser Phe Ala Ser Asn Phe 
    130                 135                 140                 


Ser Ser Tyr Lys Asp Ile Ile Thr Lys Ile Tyr Ile Ser Gly Gly Pro 
145                 150                 155                 160 


Ser Gly Glu Leu Arg Tyr Pro Ser Tyr Asn Pro Ser His Gly Trp Thr 
                165                 170                 175     


Tyr Pro Gly Arg Gly Ser Leu Gln Cys Tyr Ser Lys Ala Ala Ile Thr 
            180                 185                 190         


Ser Phe Gln Asn Ala Met Lys Ser Lys Tyr Gly Thr Ile Ala Ala Val 
        195                 200                 205             


Asn Ser Ala Trp Gly Thr Ser Leu Thr Asp Phe Ser Gln Ile Ser Pro 
    210                 215                 220                 


Pro Thr Asp Gly Asp Asn Phe Phe Thr Asn Gly Tyr Lys Thr Thr Tyr 
225                 230                 235                 240 


Gly Asn Asp Phe Leu Thr Trp Tyr Gln Ser Val Leu Thr Asn Glu Leu 
                245                 250                 255     


Ala Asn Ile Ala Ser Val Ala His Ser Cys Phe Asp Pro Val Phe Asn 
            260                 265                 270         


Val Pro Ile Gly Ala Lys Ile Ala Gly Val His Trp Leu Tyr Asn Ser 
        275                 280                 285             


Pro Thr Met Pro His Ala Ala Glu Tyr Cys Ala Gly Tyr Tyr Asn Tyr 
    290                 295                 300                 


Ser Thr Leu Leu Asp Gln Phe Lys Ala Ser Asn Leu Ala Met Thr Phe 
305                 310                 315                 320 


Thr Cys Leu Glu Met Asp Asp Ser Asn Ala Tyr Val Ser Pro Tyr Tyr 
                325                 330                 335     


Ser Ala Pro Met Thr Leu Val His Tyr Val Ala Asn Leu Ala Asn Asn 
            340                 345                 350         


Lys Gly Ile Val His Asn Gly Glu Asn Ala Leu Ala Ile Ser Asn Asn 
        355                 360                 365             


Asn Gln Ala Tyr Val Asn Cys Ala Asn Glu Leu Thr Gly Tyr Asn Phe 
    370                 375                 380                 


Ser Gly Phe Thr Leu Leu Arg Leu Ser Asn Ile Val Asn Ser Asp Gly 
385                 390                 395                 400 


Ser Val Thr Ser Glu Met Ala Pro Phe Val Ile Asn Ile Val Thr Leu 
                405                 410                 415     


Thr Pro Asn Gly Thr Ile Pro Val Thr Phe Thr Ile Asn Asn Ala Thr 
            420                 425                 430         


Thr Tyr Tyr Gly Gln Asn Val Tyr Ile Val Gly Ser Thr Ser Asp Leu 
        435                 440                 445             


Gly Asn Trp Asn Thr Thr Tyr Ala Arg Gly Pro Ala Ser Cys Pro Asn 
    450                 455                 460                 


Tyr Pro Thr Trp Thr Ile Thr Leu Asn Leu Leu Pro Gly Glu Gln Ile 
465                 470                 475                 480 


Gln Phe Lys Ala Val Lys Ile Asp Ser Ser Gly Asn Val Thr Trp Glu 
                485                 490                 495     


Gly Gly Ser Asn His Thr Tyr Thr Val Pro Thr Ser Gly Thr Gly Ser 
            500                 505                 510         


Val Thr Ile Thr Trp Gln Asn 
        515                 


<210>  2
<211>  519
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic generation

<400>  2

Ser Ile Ala Pro Asn Phe Lys Val Phe Val Met Gly Pro Leu Glu Lys 
1               5                   10                  15      


Val Thr Asp Phe Asn Ala Phe Lys Lys Gln Cys Ile Thr Leu Lys Asn 
            20                  25                  30          


Asn Gly Val Tyr Gly Ile Thr Thr Asp Ile Trp Trp Gly Tyr Val Glu 
        35                  40                  45              


Asn Ala Gly Glu Asn Gln Phe Asp Trp Ser Tyr Tyr Lys Thr Tyr Ala 
    50                  55                  60                  


Asp Thr Val Arg Ala Ala Gly Leu Lys Trp Val Pro Ile Met Ser Thr 
65                  70                  75                  80  


His Ala Cys Gly Gly Asn Val Gly Asp Thr Val Asn Ile Pro Ile Pro 
                85                  90                  95      


Ser Trp Val Trp Thr Lys Asp Thr Gln Asp Asn Met Gln Tyr Lys Asp 
            100                 105                 110         


Glu Ala Gly Asn Trp Asp Asn Glu Ala Val Ser Pro Trp Tyr Ser Gly 
        115                 120                 125             


Leu Thr Gln Leu Tyr Asn Glu Phe Tyr Ser Ser Phe Ala Ser Asn Phe 
    130                 135                 140                 


Ser Ser Tyr Lys Asp Ile Ile Thr Lys Ile Tyr Ile Ser Gly Gly Pro 
145                 150                 155                 160 


Ser Gly Glu Leu Arg Tyr Pro Ser Tyr Asn Pro Ser His Gly Trp Thr 
                165                 170                 175     


Tyr Pro Gly Arg Gly Ser Leu Gln Cys Tyr Ser Lys Ala Ala Ile Thr 
            180                 185                 190         


Ser Phe Gln Asn Ala Met Lys Ser Lys Tyr Gly Thr Ile Ala Ala Val 
        195                 200                 205             


Asn Ser Ala Trp Gly Thr Ser Leu Thr Asp Phe Leu Gln Ile Ser Pro 
    210                 215                 220                 


Pro Thr Asp Gly Asp Asn Phe Phe Thr Asn Gly Tyr Lys Thr Thr Tyr 
225                 230                 235                 240 


Gly Asn Asp Phe Leu Thr Trp Tyr Gln Ser Val Leu Thr Asn Glu Leu 
                245                 250                 255     


Ala Asn Ile Ala Ser Val Ala His Ser Cys Phe Asp Pro Val Phe Asn 
            260                 265                 270         


Val Pro Ile Gly Ala Lys Ile Ala Gly Val His Trp Leu Tyr Asn Ser 
        275                 280                 285             


Pro Thr Met Pro His Ala Ala Glu Tyr Cys Ala Gly Tyr Tyr Asn Tyr 
    290                 295                 300                 


Ser Thr Leu Leu Asp Gln Phe Lys Ala Ser Asn Leu Ala Met Thr Phe 
305                 310                 315                 320 


Thr Cys Leu Glu Met Asp Asp Ser Asn Ala Tyr Val Ser Pro Tyr Tyr 
                325                 330                 335     


Ser Ala Pro Met Thr Leu Val His Tyr Val Ala Asn Leu Ala Asn Asn 
            340                 345                 350         


Lys Gly Ile Val His Asn Gly Glu Asn Ala Leu Pro Ile Ser Asn Asn 
        355                 360                 365             


Pro Gln Ala Tyr Val Asn Cys Ala Asn Glu Leu Thr Gly Tyr Asn Phe 
    370                 375                 380                 


Ser Gly Phe Thr Leu Leu Arg Leu Ser Asn Ile Val Asn Pro Asp Gly 
385                 390                 395                 400 


Ser Val Thr Ser Glu Met Ala Pro Phe Val Ile Asn Ile Val Thr Leu 
                405                 410                 415     


Thr Pro Asn Gly Thr Ile Pro Val Thr Phe Thr Ile Asn Asn Ala Thr 
            420                 425                 430         


Thr Tyr Tyr Gly Gln Asn Val Tyr Ile Val Gly Ser Thr Ser Asp Leu 
        435                 440                 445             


Gly Asn Trp Asn Thr Thr Tyr Ala Arg Gly Pro Ala Ser Cys Pro Asn 
    450                 455                 460                 


Tyr Pro Thr Trp Thr Ile Thr Leu Asn Leu Leu Pro Gly Glu Gln Ile 
465                 470                 475                 480 


Gln Phe Lys Ala Val Lys Ile Asp Ser Ser Gly Asn Val Thr Trp Glu 
                485                 490                 495     


Gly Gly Ser Asn His Thr Tyr Thr Val Pro Thr Ser Gly Thr Gly Ser 
            500                 505                 510         


Val Thr Ile Thr Trp Gln Asn 
        515                 


<210>  3
<211>  519
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Generation

<400>  3

Ser Ile Ala Pro Asn Phe Lys Val Phe Val Met Gly Pro Leu Glu Lys 
1               5                   10                  15      


Val Thr Asp Phe Asn Ala Phe Lys Lys Gln Cys Ile Thr Leu Lys Asn 
            20                  25                  30          


Asn Gly Val Tyr Gly Ile Thr Thr Asp Ile Trp Trp Gly Tyr Val Glu 
        35                  40                  45              


Asn Ala Gly Glu Asn Gln Phe Asp Trp Ser Tyr Tyr Lys Thr Tyr Ala 
    50                  55                  60                  


Asp Thr Val Arg Ala Ala Gly Leu Lys Trp Val Pro Ile Met Ser Thr 
65                  70                  75                  80  


His Ala Cys Gly Gly Asn Val Gly Asp Thr Val Asn Ile Pro Ile Pro 
                85                  90                  95      


Ser Trp Val Trp Thr Lys Asp Thr Gln Asp Asn Met Gln Tyr Lys Asp 
            100                 105                 110         


Glu Ala Gly Asn Trp Asp Asn Glu Ala Val Ser Pro Trp Tyr Ser Gly 
        115                 120                 125             


Leu Thr Gln Leu Tyr Asn Glu Phe Tyr Ser Ser Phe Ala Ser Asn Phe 
    130                 135                 140                 


Ser Ser Tyr Lys Asp Ile Ile Thr Lys Ile Tyr Ile Ser Gly Gly Pro 
145                 150                 155                 160 


Ser Gly Glu Leu Arg Tyr Pro Ser Tyr Asn Pro Ser His Gly Trp Thr 
                165                 170                 175     


Tyr Pro Gly Arg Gly Ser Leu Gln Cys Tyr Ser Lys Ala Ala Ile Thr 
            180                 185                 190         


Ser Phe Gln Asn Ala Met Lys Ser Lys Tyr Gly Thr Ile Ala Ala Val 
        195                 200                 205             


Asn Ser Ala Trp Gly Thr Ser Leu Thr Asp Phe Leu Gln Ile Ser Pro 
    210                 215                 220                 


Pro Thr Asp Gly Asp Asn Phe Phe Thr Asn Gly Tyr Lys Thr Thr Tyr 
225                 230                 235                 240 


Gly Asn Asp Phe Leu Thr Trp Tyr Gln Ser Val Leu Thr Asn Glu Leu 
                245                 250                 255     


Ala Asn Ile Ala Ser Val Ala His Ser Cys Phe Asp Pro Val Phe Asn 
            260                 265                 270         


Val Pro Ile Gly Ala Lys Ile Ala Gly Val His Trp Leu Tyr Asn Ser 
        275                 280                 285             


Pro Thr Met Pro His Ala Ala Glu Tyr Cys Ala Gly Tyr Tyr Asn Tyr 
    290                 295                 300                 


Ser Thr Leu Leu Asp Gln Phe Lys Ala Ser Asn Leu Ala Met Thr Phe 
305                 310                 315                 320 


Thr Cys Leu Glu Met Asp Asp Ser Asn Ala Tyr Val Ser Pro Tyr Tyr 
                325                 330                 335     


Ser Ala Pro Met Thr Leu Val His Tyr Val Ala Asn Leu Ala Asn Asn 
            340                 345                 350         


Lys Gly Ile Val His Asn Gly Glu Asn Ala Leu Pro Ile Ser Asn Asn 
        355                 360                 365             


Pro Gln Ala Tyr Val Asn Cys Ala Asn Glu Leu Thr Gly Tyr Asn Phe 
    370                 375                 380                 


Ser Gly Phe Thr Leu Leu Arg Leu Ser Asn Ile Val Asn Pro Asp Gly 
385                 390                 395                 400 


Ser Val Thr Ser Glu Met Ala Pro Phe Val Ile Asn Ile Val Thr Leu 
                405                 410                 415     


Thr Pro Glu Gly Thr Ile Pro Val Thr Phe Thr Ile Asn Asn Ala Thr 
            420                 425                 430         


Thr Tyr Tyr Gly Gln Asn Val Tyr Ile Val Gly Ser Thr Ser Asp Leu 
        435                 440                 445             


Gly Asn Trp Asn Thr Thr Tyr Ala Arg Gly Pro Ala Ser Cys Pro Asn 
    450                 455                 460                 


Tyr Pro Thr Trp Thr Ile Thr Leu Asn Leu Leu Pro Gly Glu Gln Ile 
465                 470                 475                 480 


Gln Phe Lys Ala Val Lys Ile Asp Ser Ser Gly Asn Val Glu Trp Glu 
                485                 490                 495     


Gly Gly Ser Asn His Glu Tyr Thr Val Pro Thr Ser Gly Thr Gly Ser 
            500                 505                 510         


Val Thr Ile Thr Trp Gln Asn 
        515                 


