
                                SEQUENCE LISTING

<110> Michael C. Jensen
      Soheil Meshinchi

<120> COMBINATORIAL CAR T CELL AND
  HEMATOPOEITIC STEM CELL GENETIC ENGINEERING FOR SPECIFIC
  IMMUNOTHERAPY OF MYELOID LEUKEMIAS

<130> SCRI.122WO

<150> 62838808            
<151> 2019-04-25  

<160> 38

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 321
<212> DNA
<213> Artificial Sequence

<220> 
<223> M-spacer

<400> 1
ggccagccta gagaacccca ggtgtacacc ctgcctccca gccaggaaga gatgaccaag 60
aaccaggtgt ccctgacctg cctggtcaaa ggcttctacc ccagcgatat cgccgtggaa 120
tgggagagca acggccagcc cgagaacaac tacaagacca ccccccctgt gctggacagc 180
gacggcagct tcttcctgta ctcccggctg accgtggaca agagccggtg gcaggaaggc 240
aacgtcttca gctgcagcgt gatgcacgag gccctgcaca accactacac ccagaagtcc 300
ctgagcctga gcctgggcaa g                                           321

<210> 2
<211> 651
<212> DNA
<213> Artificial Sequence

<220> 
<223> L-spacer

<400> 2
gcccccgagt tcgacggcgg acccagcgtg ttcctgttcc cccccaagcc caaggacacc 60
ctgatgatca gccggacccc cgaggtgacc tgcgtggtgg tggacgtgag ccaggaagat 120
cccgaggtcc agttcaattg gtacgtggac ggcgtggaag tgcacaacgc caagaccaag 180
cccagagagg aacagttcca gagcacctac cgggtggtgt ctgtgctgac cgtgctgcac 240
caggactggc tgaacggcaa agaatacaag tgcaaggtgt ccaacaaggg cctgcccagc 300
agcatcgaaa agaccatcag caaggccaag ggccagcctc gcgagcccca ggtgtacacc 360
ctgcctccct cccaggaaga gatgaccaag aaccaggtgt ccctgacctg cctggtgaag 420
ggcttctacc ccagcgacat cgccgtggag tgggagagca acggccagcc tgagaacaac 480
tacaagacca cccctcccgt gctggacagc gacggcagct tcttcctgta cagccggctg 540
accgtggaca agagccggtg gcaggaaggc aacgtcttta gctgcagcgt gatgcacgag 600
gccctgcaca accactacac ccagaagagc ctgagcctgt ccctgggcaa g          651

<210> 3
<211> 90
<212> PRT
<213> Artificial Sequence

<220> 
<223> M-spacer

<400> 3
Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
 1               5                  10                  15      
Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn
            20                  25                  30          
Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
        35                  40                  45              
Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn
    50                  55                  60                  
Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr
65                  70                  75                  80  
Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
                85                  90  

<210> 4
<211> 196
<212> PRT
<213> Artificial Sequence

<220> 
<223> L-spacer

<400> 4
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser
 1               5                  10                  15      
Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu
            20                  25                  30          
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser Thr
        35                  40                  45              
Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn
    50                  55                  60                  
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser
65                  70                  75                  80  
Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln
                85                  90                  95      
Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val
            100                 105                 110         
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val
        115                 120                 125             
Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro
    130                 135                 140                 
Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr
145                 150                 155                 160 
Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val
                165                 170                 175     
Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu
            180                 185                 190         
Ser Leu Gly Lys
        195

<210> 5
<211> 117
<212> PRT
<213> Artificial Sequence

<220> 
<223> Anti-CD33 VH domain

<400> 5
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala
 1               5                  10                  15      
Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr
            20                  25                  30          
Asp Ile Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile
        35                  40                  45              
Gly Trp Ile Tyr Pro Gly Asp Gly Ser Thr Lys Tyr Asn Glu Lys Phe
    50                  55                  60                  
Lys Ala Lys Ala Thr Leu Thr Ala Asp Thr Ser Thr Ser Thr Ala Tyr
65                  70                  75                  80  
Met Glu Leu Arg Ser Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95      
Ala Ser Gly Tyr Glu Asp Ala Met Asp Tyr Trp Gly Gln Gly Thr Thr
            100                 105                 110         
Val Thr Val Ser Ser
        115

<210> 6
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH-CDR1

<400> 6
Asn Tyr Asp Ile Asn
 1               5

<210> 7
<211> 17
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH-CDR2

<400> 7
Trp Ile Tyr Pro Gly Asp Gly Ser Thr Lys Tyr Asn Glu Lys Phe Lys
 1               5                  10                  15      
Ala


<210> 8
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH-CDR3

<400> 8
Gly Tyr Glu Asp Ala Met Asp Tyr
 1               5

<210> 9
<211> 107
<212> PRT
<213> Artificial Sequence

<220> 
<223> Anti-CD33 VL domain

<400> 9
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
 1               5                  10                  15      
Asp Arg Val Thr Ile Asn Cys Lys Ala Ser Gln Asp Ile Asn Ser Tyr
            20                  25                  30          
Leu Ser Trp Phe Gln Gln Lys Pro Gly Lys Ala Pro Lys Thr Leu Ile
        35                  40                  45              
Tyr Arg Ala Asn Arg Leu Val Asp Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Gln Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Tyr Asp Glu Phe Pro Leu
                85                  90                  95      
Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
            100                 105

<210> 10
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL-CDR1

<400> 10
Lys Ala Ser Gln Asp Ile Asn Ser Tyr Leu Ser
 1               5                  10

<210> 11
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL-CDR2

<400> 11
Arg Ala Asn Asn Arg Leu Val Asp
 1               5

<210> 12
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL-CDR3

<400> 12
Leu Gln Tyr Asp Glu Phe Pro Leu Thr
 1               5

<210> 13
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> scFv linker

<400> 13
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
 1               5                  10                  15

<210> 14
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> S spacer

<400> 14
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro
 1               5                  10

<210> 15
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> S spacer

<400> 15
gaatctaagt acggaccgcc ctgcccccct tgccct                           36

<210> 16
<211> 119
<212> PRT
<213> Artificial Sequence

<220> 
<223> M spacer

<400> 16
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Gly Gln Pro Arg
 1               5                  10                  15      
Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys
            20                  25                  30          
Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp
        35                  40                  45              
Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys
    50                  55                  60                  
Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser
65                  70                  75                  80  
Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser
                85                  90                  95      
Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser
            100                 105                 110         
Leu Ser Leu Ser Leu Gly Lys
        115

<210> 17
<211> 357
<212> DNA
<213> Artificial Sequence

<220> 
<223> M spacer

<400> 17
gaatctaagt acggaccgcc ctgcccccct tgccctggcc agcctagaga accccaggtg 60
tacaccctgc ctcccagcca ggaagagatg accaagaacc aggtgtccct gacctgcctg 120
gtcaaaggct tctaccccag cgatatcgcc gtggaatggg agagcaacgg ccagcccgag 180
aacaactaca agaccacccc ccctgtgctg gacagcgacg gcagcttctt cctgtactcc 240
cggctgaccg tggacaagag ccggtggcag gaaggcaacg tcttcagctg cagcgtgatg 300
cacgaggccc tgcacaacca ctacacccag aagtccctga gcctgagcct gggcaag    357

<210> 18
<211> 229
<212> PRT
<213> Artificial Sequence

<220> 
<223> L spacer

<400> 18
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe
 1               5                  10                  15      
Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
            20                  25                  30          
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
        35                  40                  45              
Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val
    50                  55                  60                  
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser
65                  70                  75                  80  
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
                85                  90                  95      
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser
            100                 105                 110         
Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
        115                 120                 125             
Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln
    130                 135                 140                 
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
145                 150                 155                 160 
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
                165                 170                 175     
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu
            180                 185                 190         
Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser
        195                 200                 205             
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
    210                 215                 220                 
Leu Ser Leu Gly Lys
225

<210> 19
<211> 685
<212> DNA
<213> Artificial Sequence

<220> 
<223> L spacer

<400> 19
atctaagtac ggaccgccct gccccccttg ccctgccccc gagttcgacg gcggacccag 60
cgtgttcctg ttccccccca agcccaagga caccctgatg atcagccgga cccccgaggt 120
gacctgcgtg gtggtggacg tgagccagga agatcccgag gtccagttca attggtacgt 180
ggacggcgtg gaagtgcaca acgccaagac caagcccaga gaggaacagt tccagagcac 240
ctaccgggtg gtgtctgtgc tgaccgtgct gcaccaggac tggctgaacg gcaaagaata 300
caagtgcaag gtgtccaaca agggcctgcc cagcagcatc gaaaagacca tcagcaaggc 360
caagggccag cctcgcgagc cccaggtgta caccctgcct ccctcccagg aagagatgac 420
caagaaccag gtgtccctga cctgcctggt gaagggcttc taccccagcg acatcgccgt 480
ggagtgggag agcaacggcc agcctgagaa caactacaag accacccctc ccgtgctgga 540
cagcgacggc agcttcttcc tgtacagccg gctgaccgtg gacaagagcc ggtggcagga 600
aggcaacgtc tttagctgca gcgtgatgca cgaggccctg cacaaccact acacccagaa 660
gagcctgagc ctgtccctgg gcaag                                       685

<210> 20
<211> 28
<212> PRT
<213> Artificial Sequence

<220> 
<223> CD28tm

<400> 20
Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser
 1               5                  10                  15      
Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val
            20                  25

<210> 21
<211> 84
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD28tm

<400> 21
atgttctggg tgctggtggt ggtcggaggc gtgctggcct gctacagcct gctggtcacc 60
gtggccttca tcatcttttg ggtg                                        84

<210> 22
<211> 126
<212> DNA
<213> Artificial Sequence

<220> 
<223> 41-BB

<400> 22
aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa 60
actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt 120
gaactg                                                            126

<210> 23
<211> 336
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD3zeta

<400> 23
cgggtgaagt tcagcagaag cgccgacgcc cctgcctacc agcagggcca gaatcagctg 60
tacaacgagc tgaacctggg cagaagggaa gagtacgacg tcctggataa gcggagaggc 120
cgggaccctg agatgggcgg caagcctcgg cggaagaacc cccaggaagg cctgtataac 180
gaactgcaga aagacaagat ggccgaggcc tacagcgaga tcggcatgaa gggcgagcgg 240
aggcggggca agggccacga cggcctgtat cagggcctgt ccaccgccac caaggatacc 300
tacgacgccc tgcacatgca ggccctgccc ccaagg                           336

<210> 24
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A

<400> 24
ctcgagggcg gcggagaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat 60
cccggcccta gg                                                     72

<210> 25
<211> 1005
<212> DNA
<213> Artificial Sequence

<220> 
<223> EGFRt

<400> 25
cgcaaagtgt gtaacggaat aggtattggt gaatttaaag actcactctc cataaatgct 60
acgaatatta aacacttcaa aaactgcacc tccatcagtg gcgatctcca catcctgccg 120
gtggcattta ggggtgactc cttcacacat actcctcctc tggatccaca ggaactggat 180
attctgaaaa ccgtaaagga aatcacaggg tttttgctga ttcaggcttg gcctgaaaac 240
aggacggacc tccatgcctt tgagaaccta gaaatcatac gcggcaggac caagcaacat 300
ggtcagtttt ctcttgcagt cgtcagcctg aacataacat ccttgggatt acgctccctc 360
aaggagataa gtgatggaga tgtgataatt tcaggaaaca aaaatttgtg ctatgcaaat 420
acaataaact ggaaaaaact gtttgggacc tccggtcaga aaaccaaaat tataagcaac 480
agaggtgaaa acagctgcaa ggccacaggc caggtctgcc atgccttgtg ctcccccgag 540
ggctgctggg gcccggagcc cagggactgc gtctcttgcc ggaatgtcag ccgaggcagg 600
gaatgcgtgg acaagtgcaa ccttctggag ggtgagccaa gggagtttgt ggagaactct 660
gagtgcatac agtgccaccc agagtgcctg cctcaggcca tgaacatcac ctgcacagga 720
cggggaccag acaactgtat ccagtgtgcc cactacattg acggccccca ctgcgtcaag 780
acctgcccgg caggagtcat gggagaaaac aacaccctgg tctggaagta cgcagacgcc 840
ggccatgtgt gccacctgtg ccatccaaac tgcacctacg gatgcactgg gccaggtctt 900
gaaggctgtc caacgaatgg gcctaagatc ccgtccatcg ccactgggat ggtgggggcc 960
ctcctcttgc tgctggtggt ggccctgggg atcggcctct tcatg                 1005

<210> 26
<211> 187
<212> PRT
<213> Artificial Sequence

<220> 
<223> DHFRdm

<400> 26
Met Val Gly Ser Leu Asn Cys Ile Val Ala Val Ser Gln Asn Met Gly
 1               5                  10                  15      
Ile Gly Lys Asn Gly Asp Phe Pro Trp Pro Pro Leu Arg Asn Glu Ser
            20                  25                  30          
Arg Tyr Phe Gln Arg Met Thr Thr Thr Ser Ser Val Glu Gly Lys Gln
        35                  40                  45              
Asn Leu Val Ile Met Gly Lys Lys Thr Trp Phe Ser Ile Pro Glu Lys
    50                  55                  60                  
Asn Arg Pro Leu Lys Gly Arg Ile Asn Leu Val Leu Ser Arg Glu Leu
65                  70                  75                  80  
Lys Glu Pro Pro Gln Gly Ala His Phe Leu Ser Arg Ser Leu Asp Asp
                85                  90                  95      
Ala Leu Lys Leu Thr Glu Gln Pro Glu Leu Ala Asn Lys Val Asp Met
            100                 105                 110         
Val Trp Ile Val Gly Gly Ser Ser Val Tyr Lys Glu Ala Met Asn His
        115                 120                 125             
Pro Gly His Leu Lys Leu Phe Val Thr Arg Ile Met Gln Asp Phe Glu
    130                 135                 140                 
Ser Asp Thr Phe Phe Pro Glu Ile Asp Leu Glu Lys Tyr Lys Leu Leu
145                 150                 155                 160 
Pro Glu Tyr Pro Gly Val Leu Ser Asp Val Gln Glu Glu Lys Gly Ile
                165                 170                 175     
Lys Tyr Lys Phe Glu Val Tyr Glu Lys Asn Asp
            180                 185

<210> 27
<211> 564
<212> DNA
<213> Artificial Sequence

<220> 
<223> DHFRdm

<400> 27
atggttggtt cgctaaactg catcgtcgct gtgtcccaga acatgggcat cggcaagaac 60
ggggacttcc cctggccacc gctcaggaat gaatccagat atttccagag aatgaccaca 120
acctcttcag tagaaggtaa acagaatctg gtgattatgg gtaagaagac ctggttctcc 180
attcctgaga agaatcgacc tttaaagggt agaattaatt tagttctcag cagagaactc 240
aaggaacctc cacaaggagc tcattttctt tccagaagtc tagatgatgc cttaaaactt 300
actgaacaac cagaattagc aaataaagta gacatggtct ggatagttgg tggcagttct 360
gtttataagg aagccatgaa tcacccaggc catcttaaac tatttgtgac aaggatcatg 420
caagactttg aaagtgacac gttttttcca gaaattgatt tggagaaata taaacttctg 480
ccagaatacc caggtgttct ctctgatgtc caggaggaga aaggcattaa gtacaaattt 540
gaagtatatg agaagaatga ttaa                                        564

<210> 28
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> CD19 signal sequence

<400> 28
Met Pro Pro Pro Arg Leu Leu Phe Phe Leu Leu Phe Leu Thr Pro
 1               5                  10                  15

<210> 29
<211> 22
<212> PRT
<213> Artificial Sequence

<220> 
<223> GMCSFR signal sequence

<400> 29
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro
 1               5                  10                  15      
Ala Phe Leu Leu Ile Pro
            20

<210> 30
<211> 972
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD19t

<400> 30
atgccacctc ctcgcctcct cttcttcctc ctcttcctca cccccatgga agtcaggccc 60
gaggaacctc tagtggtgaa ggtggaagag ggagataacg ctgtgctgca gtgcctcaag 120
gggacctcag atggccccac tcagcagctg acctggtctc gggagtcccc gcttaaaccc 180
ttcttaaaac tcagcctggg gctgccaggc ctgggaatcc acatgaggcc cctggccatc 240
tggcttttca tcttcaacgt ctctcaacag atggggggct tctacctgtg ccagccgggg 300
cccccctctg agaaggcctg gcagcctggc tggacagtca atgtggaggg cagcggggag 360
ctgttccggt ggaatgtttc ggacctaggt ggcctgggct gtggcctgaa gaacaggtcc 420
tcagagggcc ccagctcccc ttccgggaag ctcatgagcc ccaagctgta tgtgtgggcc 480
aaagaccgcc ctgagatctg ggagggagag cctccgtgtg tcccaccgag ggacagcctg 540
aaccagagcc tcagccagga cctcaccatg gcccctggct ccacactctg gctgtcctgt 600
ggggtacccc ctgactctgt gtccaggggc cccctctcct ggacccatgt gcaccccaag 660
gggcctaagt cattgctgag cctagagctg aaggacgatc gcccggccag agatatgtgg 720
gtaatggaga cgggtctgtt gttgccccgg gccacagctc aagacgctgg aaagtattat 780
tgtcaccgtg gcaacctgac catgtcattc cacctggaga tcactgctcg gccagtacta 840
tggcactggc tgctgaggac tggtggctgg aaggtctcag ctgtgacttt ggcttatctg 900
atcttctgcc tgtgttccct tgtgggcatt cttcatcttc aaagagccct ggtcctgagg 960
aggaaaagat aa                                                     972

<210> 31
<211> 323
<212> PRT
<213> Artificial Sequence

<220> 
<223> CD19t

<400> 31
Met Pro Pro Pro Arg Leu Leu Phe Phe Leu Leu Phe Leu Thr Pro Met
 1               5                  10                  15      
Glu Val Arg Pro Glu Glu Pro Leu Val Val Lys Val Glu Glu Gly Asp
            20                  25                  30          
Asn Ala Val Leu Gln Cys Leu Lys Gly Thr Ser Asp Gly Pro Thr Gln
        35                  40                  45              
Gln Leu Thr Trp Ser Arg Glu Ser Pro Leu Lys Pro Phe Leu Lys Leu
    50                  55                  60                  
Ser Leu Gly Leu Pro Gly Leu Gly Ile His Met Arg Pro Leu Ala Ile
65                  70                  75                  80  
Trp Leu Phe Ile Phe Asn Val Ser Gln Gln Met Gly Gly Phe Tyr Leu
                85                  90                  95      
Cys Gln Pro Gly Pro Pro Ser Glu Lys Ala Trp Gln Pro Gly Trp Thr
            100                 105                 110         
Val Asn Val Glu Gly Ser Gly Glu Leu Phe Arg Trp Asn Val Ser Asp
        115                 120                 125             
Leu Gly Gly Leu Gly Cys Gly Leu Lys Asn Arg Ser Ser Glu Gly Pro
    130                 135                 140                 
Ser Ser Pro Ser Gly Lys Leu Met Ser Pro Lys Leu Tyr Val Trp Ala
145                 150                 155                 160 
Lys Asp Arg Pro Glu Ile Trp Glu Gly Glu Pro Pro Cys Val Pro Pro
                165                 170                 175     
Arg Asp Ser Leu Asn Gln Ser Leu Ser Gln Asp Leu Thr Met Ala Pro
            180                 185                 190         
Gly Ser Thr Leu Trp Leu Ser Cys Gly Val Pro Pro Asp Ser Val Ser
        195                 200                 205             
Arg Gly Pro Leu Ser Trp Thr His Val His Pro Lys Gly Pro Lys Ser
    210                 215                 220                 
Leu Leu Ser Leu Glu Leu Lys Asp Asp Arg Pro Ala Arg Asp Met Trp
225                 230                 235                 240 
Val Met Glu Thr Gly Leu Leu Leu Pro Arg Ala Thr Ala Gln Asp Ala
                245                 250                 255     
Gly Lys Tyr Tyr Cys His Arg Gly Asn Leu Thr Met Ser Phe His Leu
            260                 265                 270         
Glu Ile Thr Ala Arg Pro Val Leu Trp His Trp Leu Leu Arg Thr Gly
        275                 280                 285             
Gly Trp Lys Val Ser Ala Val Thr Leu Ala Tyr Leu Ile Phe Cys Leu
    290                 295                 300                 
Cys Ser Leu Val Gly Ile Leu His Leu Gln Arg Ala Leu Val Leu Arg
305                 310                 315                 320 
Arg Lys Arg

<210> 32
<211> 66
<212> DNA
<213> Artificial Sequence

<220> 
<223> GM-CSF receptor-chain signal sequence

<400> 32
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg 60
atccca                                                            66

<210> 33
<211> 22
<212> PRT
<213> Artificial Sequence

<220> 
<223> GM-CSF receptor-chain signal sequence

<400> 33
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro
 1               5                  10                  15      
Ala Phe Leu Leu Ile Pro
            20

<210> 34
<211> 2175
<212> DNA
<213> Artificial Sequence

<220> 
<223> pJ03975_CD33VH-VL-IgG4Hinge-CD28tm-4-1BB-T2A-EGFRt
      _epHIV72

<400> 34
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg 60
atcccacagg ttcagctggt gcagtctgga gctgaggtga agaagcctgg ggcctcagtg 120
aaggtctcct gcaaggcttc tggttacacc tttaccaatt atgatataaa ttgggtgaga 180
caggcccctg gacaagggct tgagtggatt ggatggattt atcctggaga tggtagtacc 240
aaatataatg agaaattcaa ggccaaggct accctgacag ctgacacatc caccagcaca 300
gcctacatgg agctgaggag cctgagatct gatgacacag ctgtgtatta ctgtgcttct 360
ggatatgaag atgctatgga ctactggggg caagggacca cagtcacagt ctcctcaggt 420
ggcggtggca gcggcggtgg tggttccgga ggcggcggtt ctgacatcca gatgacccag 480
tctccatcct cactgtctgc atctgtagga gacagagtca ccatcaattg taaggctagt 540
caggacatta atagctattt gagctggttt cagcagaaac cagggaaagc ccctaagacc 600
ctgatctata gagcaaatag attggtagat ggggtcccat caaggttctc tggcagtgga 660
tctgggcaag attatactct caccatcagc agcctgcagc ctgaagattt tgcaacttat 720
tactgcttgc agtatgatga gtttcctctc acatttggag gagggaccaa ggtggagatc 780
aaagaatcta agtacggacc gccctgcccc ccttgcccta tgttctgggt gctggtggtg 840
gtcggaggcg tgctggcctg ctacagcctg ctggtcaccg tggccttcat catcttttgg 900
gtgaaacggg gcagaaagaa actcctgtat atattcaaac aaccatttat gagaccagta 960
caaactactc aagaggaaga tggctgtagc tgccgatttc cagaagaaga agaaggagga 1020
tgtgaactgc tcgagggcgg cggagagggc agaggaagtc ttctaacatg cggtgacgtg 1080
gaggagaatc ccggccctag gatgcttctc ctggtgacaa gccttctgct ctgtgagtta 1140
ccacacccag cattcctcct gatcccacgc aaagtgtgta acggaatagg tattggtgaa 1200
tttaaagact cactctccat aaatgctacg aatattaaac acttcaaaaa ctgcacctcc 1260
atcagtggcg atctccacat cctgccggtg gcatttaggg gtgactcctt cacacatact 1320
cctcctctgg atccacagga actggatatt ctgaaaaccg taaaggaaat cacagggttt 1380
ttgctgattc aggcttggcc tgaaaacagg acggacctcc atgcctttga gaacctagaa 1440
atcatacgcg gcaggaccaa gcaacatggt cagttttctc ttgcagtcgt cagcctgaac 1500
ataacatcct tgggattacg ctccctcaag gagataagtg atggagatgt gataatttca 1560
ggaaacaaaa atttgtgcta tgcaaataca ataaactgga aaaaactgtt tgggacctcc 1620
ggtcagaaaa ccaaaattat aagcaacaga ggtgaaaaca gctgcaaggc cacaggccag 1680
gtctgccatg ccttgtgctc ccccgagggc tgctggggcc cggagcccag ggactgcgtc 1740
tcttgccgga atgtcagccg aggcagggaa tgcgtggaca agtgcaacct tctggagggt 1800
gagccaaggg agtttgtgga gaactctgag tgcatacagt gccacccaga gtgcctgcct 1860
caggccatga acatcacctg cacaggacgg ggaccagaca actgtatcca gtgtgcccac 1920
tacattgacg gcccccactg cgtcaagacc tgcccggcag gagtcatggg agaaaacaac 1980
accctggtct ggaagtacgc agacgccggc catgtgtgcc acctgtgcca tccaaactgc 2040
acctacggat gcactgggcc aggtcttgaa ggctgtccaa cgaatgggcc taagatcccg 2100
tccatcgcca ctgggatggt gggggccctc ctcttgctgc tggtggtggc cctggggatc 2160
ggcctcttca tgtga                                                  2175

<210> 35
<211> 724
<212> PRT
<213> Artificial Sequence

<220> 
<223> pJ03975_CD33VH-VL-IgG4Hinge-CD28tm-4-1BB-T2A-EGFRt
      _epHIV72

<400> 35
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro
 1               5                  10                  15      
Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu
            20                  25                  30          
Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly
        35                  40                  45              
Tyr Thr Phe Thr Asn Tyr Asp Ile Asn Trp Val Arg Gln Ala Pro Gly
    50                  55                  60                  
Gln Gly Leu Glu Trp Ile Gly Trp Ile Tyr Pro Gly Asp Gly Ser Thr
65                  70                  75                  80  
Lys Tyr Asn Glu Lys Phe Lys Ala Lys Ala Thr Leu Thr Ala Asp Thr
                85                  90                  95      
Ser Thr Ser Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp Asp
            100                 105                 110         
Thr Ala Val Tyr Tyr Cys Ala Ser Gly Tyr Glu Asp Ala Met Asp Tyr
        115                 120                 125             
Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser
    130                 135                 140                 
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln
145                 150                 155                 160 
Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Asn
                165                 170                 175     
Cys Lys Ala Ser Gln Asp Ile Asn Ser Tyr Leu Ser Trp Phe Gln Gln
            180                 185                 190         
Lys Pro Gly Lys Ala Pro Lys Thr Leu Ile Tyr Arg Ala Asn Arg Leu
        195                 200                 205             
Val Asp Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Gln Asp
    210                 215                 220                 
Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr
225                 230                 235                 240 
Tyr Cys Leu Gln Tyr Asp Glu Phe Pro Leu Thr Phe Gly Gly Gly Thr
                245                 250                 255     
Lys Val Glu Ile Lys Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys
            260                 265                 270         
Pro Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr
        275                 280                 285             
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly
    290                 295                 300                 
Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val
305                 310                 315                 320 
Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu
                325                 330                 335     
Glu Glu Gly Gly Cys Glu Leu Leu Glu Gly Gly Gly Glu Gly Arg Gly
            340                 345                 350         
Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Arg Met
        355                 360                 365             
Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala
    370                 375                 380                 
Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu
385                 390                 395                 400 
Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys
                405                 410                 415     
Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe
            420                 425                 430         
Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu
        435                 440                 445             
Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln
    450                 455                 460                 
Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu
465                 470                 475                 480 
Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val
                485                 490                 495     
Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile
            500                 505                 510         
Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala
        515                 520                 525             
Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr
    530                 535                 540                 
Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln
545                 550                 555                 560 
Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro
                565                 570                 575     
Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val
            580                 585                 590         
Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn
        595                 600                 605             
Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn
    610                 615                 620                 
Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His
625                 630                 635                 640 
Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val Met
                645                 650                 655     
Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val
            660                 665                 670         
Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly
        675                 680                 685             
Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr
    690                 695                 700                 
Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile
705                 710                 715                 720 
Gly Leu Phe Met

<210> 36
<211> 2616
<212> DNA
<213> Artificial Sequence

<220> 
<223> pJ04043-CD33scFv-VH-VL-CD8hinge-tm-41BB-zeta-T2A-E
      GFRt-epHIV72

<400> 36
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg 60
atcccacagg ttcagctggt gcagtctgga gctgaggtga agaagcctgg ggcctcagtg 120
aaggtctcct gcaaggcttc tggttacacc tttaccaatt atgatataaa ttgggtgaga 180
caggcccctg gacaagggct tgagtggatt ggatggattt atcctggaga tggtagtacc 240
aaatataatg agaaattcaa ggccaaggct accctgacag ctgacacatc caccagcaca 300
gcctacatgg agctgaggag cctgagatct gatgacacag ctgtgtatta ctgtgcttct 360
ggatatgaag atgctatgga ctactggggg caagggacca cagtcacagt ctcctcaggt 420
ggcggtggca gcggcggtgg tggttccgga ggcggcggtt ctgacatcca gatgacccag 480
tctccatcct cactgtctgc atctgtagga gacagagtca ccatcaattg taaggctagt 540
caggacatta atagctattt gagctggttt cagcagaaac cagggaaagc ccctaagacc 600
ctgatctata gagcaaatag attggtagat ggggtcccat caaggttctc tggcagtgga 660
tctgggcaag attatactct caccatcagc agcctgcagc ctgaagattt tgcaacttat 720
tactgcttgc agtatgatga gtttcctctc acatttggag gagggaccaa ggtggagatc 780
aaagaatcta agtacggacc ggccaagcct accaccaccc ctgcccctag acctccaaca 840
cccgccccaa caatcgccag ccagcctctg tctctgaggc ccgaggcttg tagaccagct 900
gctggcggag ccgtgcacac cagaggactg gatttcgcct gcgacatcta catctgggcc 960
cctctggccg gcacatgtgg cgtgctgctg ctgagcctcg tgatcaccaa acggggcaga 1020
aagaaactcc tgtatatatt caaacaacca tttatgagac cagtacaaac tactcaagag 1080
gaagatggct gtagctgccg atttccagaa gaagaagaag gaggatgtga actgcgggtg 1140
aagttcagca gaagcgccga cgcccctgcc taccagcagg gccagaatca gctgtacaac 1200
gagctgaacc tgggcagaag ggaagagtac gacgtcctgg ataagcggag aggccgggac 1260
cctgagatgg gcggcaagcc tcggcggaag aacccccagg aaggcctgta taacgaactg 1320
cagaaagaca agatggccga ggcctacagc gagatcggca tgaagggcga gcggaggcgg 1380
ggcaagggcc acgacggcct gtatcagggc ctgtccaccg ccaccaagga tacctacgac 1440
gccctgcaca tgcaggccct gcccccaagg ctcgagggcg gcggagaggg cagaggaagt 1500
cttctaacat gcggtgacgt ggaggagaat cccggcccta ggatgcttct cctggtgaca 1560
agccttctgc tctgtgagtt accacaccca gcattcctcc tgatcccacg caaagtgtgt 1620
aacggaatag gtattggtga atttaaagac tcactctcca taaatgctac gaatattaaa 1680
cacttcaaaa actgcacctc catcagtggc gatctccaca tcctgccggt ggcatttagg 1740
ggtgactcct tcacacatac tcctcctctg gatccacagg aactggatat tctgaaaacc 1800
gtaaaggaaa tcacagggtt tttgctgatt caggcttggc ctgaaaacag gacggacctc 1860
catgcctttg agaacctaga aatcatacgc ggcaggacca agcaacatgg tcagttttct 1920
cttgcagtcg tcagcctgaa cataacatcc ttgggattac gctccctcaa ggagataagt 1980
gatggagatg tgataatttc aggaaacaaa aatttgtgct atgcaaatac aataaactgg 2040
aaaaaactgt ttgggacctc cggtcagaaa accaaaatta taagcaacag aggtgaaaac 2100
agctgcaagg ccacaggcca ggtctgccat gccttgtgct cccccgaggg ctgctggggc 2160
ccggagccca gggactgcgt ctcttgccgg aatgtcagcc gaggcaggga atgcgtggac 2220
aagtgcaacc ttctggaggg tgagccaagg gagtttgtgg agaactctga gtgcatacag 2280
tgccacccag agtgcctgcc tcaggccatg aacatcacct gcacaggacg gggaccagac 2340
aactgtatcc agtgtgccca ctacattgac ggcccccact gcgtcaagac ctgcccggca 2400
ggagtcatgg gagaaaacaa caccctggtc tggaagtacg cagacgccgg ccatgtgtgc 2460
cacctgtgcc atccaaactg cacctacgga tgcactgggc caggtcttga aggctgtcca 2520
acgaatgggc ctaagatccc gtccatcgcc actgggatgg tgggggccct cctcttgctg 2580
ctggtggtgg ccctggggat cggcctcttc atgtga                           2616

<210> 37
<211> 871
<212> PRT
<213> Artificial Sequence

<220> 
<223> pJ04043-CD33scFv-VH-VL-CD8hinge-tm-41BB-zeta-T2A-E
      GFRt-epHIV72

<400> 37
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro
 1               5                  10                  15      
Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu
            20                  25                  30          
Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly
        35                  40                  45              
Tyr Thr Phe Thr Asn Tyr Asp Ile Asn Trp Val Arg Gln Ala Pro Gly
    50                  55                  60                  
Gln Gly Leu Glu Trp Ile Gly Trp Ile Tyr Pro Gly Asp Gly Ser Thr
65                  70                  75                  80  
Lys Tyr Asn Glu Lys Phe Lys Ala Lys Ala Thr Leu Thr Ala Asp Thr
                85                  90                  95      
Ser Thr Ser Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp Asp
            100                 105                 110         
Thr Ala Val Tyr Tyr Cys Ala Ser Gly Tyr Glu Asp Ala Met Asp Tyr
        115                 120                 125             
Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser
    130                 135                 140                 
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln
145                 150                 155                 160 
Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Asn
                165                 170                 175     
Cys Lys Ala Ser Gln Asp Ile Asn Ser Tyr Leu Ser Trp Phe Gln Gln
            180                 185                 190         
Lys Pro Gly Lys Ala Pro Lys Thr Leu Ile Tyr Arg Ala Asn Arg Leu
        195                 200                 205             
Val Asp Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Gln Asp
    210                 215                 220                 
Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr
225                 230                 235                 240 
Tyr Cys Leu Gln Tyr Asp Glu Phe Pro Leu Thr Phe Gly Gly Gly Thr
                245                 250                 255     
Lys Val Glu Ile Lys Glu Ser Lys Tyr Gly Pro Ala Lys Pro Thr Thr
            260                 265                 270         
Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln
        275                 280                 285             
Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala
    290                 295                 300                 
Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala
305                 310                 315                 320 
Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr
                325                 330                 335     
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met
            340                 345                 350         
Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe
        355                 360                 365             
Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg
    370                 375                 380                 
Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn
385                 390                 395                 400 
Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg
                405                 410                 415     
Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro
            420                 425                 430         
Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala
        435                 440                 445             
Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His
    450                 455                 460                 
Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp
465                 470                 475                 480 
Ala Leu His Met Gln Ala Leu Pro Pro Arg Leu Glu Gly Gly Gly Glu
                485                 490                 495     
Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly
            500                 505                 510         
Pro Arg Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro
        515                 520                 525             
His Pro Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly
    530                 535                 540                 
Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys
545                 550                 555                 560 
His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro
                565                 570                 575     
Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro
            580                 585                 590         
Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu
        595                 600                 605             
Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu
    610                 615                 620                 
Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser
625                 630                 635                 640 
Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu
                645                 650                 655     
Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu
            660                 665                 670         
Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly
        675                 680                 685             
Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala
    690                 695                 700                 
Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly
705                 710                 715                 720 
Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg
                725                 730                 735     
Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe
            740                 745                 750         
Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln
        755                 760                 765             
Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln
    770                 775                 780                 
Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala
785                 790                 795                 800 
Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala
                805                 810                 815     
Gly His Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr
            820                 825                 830         
Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser
        835                 840                 845             
Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala
    850                 855                 860                 
Leu Gly Ile Gly Leu Phe Met
865                 870

<210> 38
<211> 71
<212> PRT
<213> Artificial Sequence

<220> 
<223> CD8a hinge and TM

<400> 38
Gly Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro
 1               5                  10                  15      
Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys
            20                  25                  30          
Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala
        35                  40                  45              
Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu
    50                  55                  60                  
Leu Leu Ser Leu Val Ile Thr
65                  70
