                         SEQUENCE LISTING

<110>  Nanyang Technological University
       Northwestern University
 
<120>  MEMBRANE PROTEASE-BASED METHODS FOR DETECTION OF BACTERIA

<130>  SP101010WO

<150>  US 62/838,516
<151>  2019-04-25

<160>  54    

<170>  PatentIn version 3.5

<210>  1
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage site FRRV

<400>  1

Phe Arg Arg Val 
1               


<210>  2
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage site FRRY

<400>  2

Phe Arg Arg Tyr 
1               


<210>  3
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage site FRRA

<400>  3

Phe Arg Arg Ala 
1               


<210>  4
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage site YRRA

<400>  4

Tyr Arg Arg Ala 
1               


<210>  5
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage site ARRA

<400>  5

Ala Arg Arg Ala 
1               


<210>  6
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cathelicidin LL37

<400>  6

Leu Leu Gly Asp Phe Phe Arg Lys Ser Lys Glu Lys Ile Gly Lys Glu 
1               5                   10                  15      


Phe Lys Arg Ile Val Gln Arg Ile Lys Asp Phe Leu Arg Asn Leu Val 
            20                  25                  30          


Pro Arg Thr Glu Ser 
        35          


<210>  7
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LL37FRRV

<400>  7

Cys Leu Leu Gly Asp Phe Phe Arg Arg Val Lys Glu Lys Ile Gly 
1               5                   10                  15  


<210>  8
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LL37FRRY

<400>  8

Cys Leu Leu Gly Asp Phe Phe Arg Arg Tyr Lys Glu Lys Ile Gly 
1               5                   10                  15  


<210>  9
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LL37FRRA

<400>  9

Cys Leu Leu Gly Asp Phe Phe Arg Arg Ala Lys Glu Lys Ile Gly 
1               5                   10                  15  


<210>  10
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LL37YRRA

<400>  10

Cys Leu Leu Gly Asp Phe Tyr Arg Arg Ala Lys Glu Lys Ile Gly 
1               5                   10                  15  


<210>  11
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LL37ARRA

<400>  11

Cys Leu Leu Gly Asp Phe Ala Arg Arg Ala Lys Glu Lys Ile Gly 
1               5                   10                  15  


<210>  12
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LL37ARKA

<400>  12

Cys Leu Leu Gly Asp Phe Ala Arg Lys Ala Lys Glu Lys Ile Gly 
1               5                   10                  15  


<210>  13
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LL37FRRV without Cysteine

<400>  13

Leu Leu Gly Asp Phe Phe Arg Arg Val Lys Glu Lys Ile Gly 
1               5                   10                  


<210>  14
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  L37FRRY without Cysteine

<400>  14

Leu Leu Gly Asp Phe Phe Arg Arg Tyr Lys Glu Lys Ile Gly 
1               5                   10                  


<210>  15
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LL37FRRA without Cysteine

<400>  15

Leu Leu Gly Asp Phe Phe Arg Arg Ala Lys Glu Lys Ile Gly 
1               5                   10                  


<210>  16
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LL37YRRA without Cysteine

<400>  16

Leu Leu Gly Asp Phe Tyr Arg Arg Ala Lys Glu Lys Ile Gly 
1               5                   10                  


<210>  17
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LL37ARRA without Cysteine

<400>  17

Leu Leu Gly Asp Phe Ala Arg Arg Ala Lys Glu Lys Ile Gly 
1               5                   10                  


<210>  18
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cecropin A

<400>  18

Lys Trp Lys Leu Phe Lys Lys Ile Glu Lys Val Gly Gln Asn Ile Arg 
1               5                   10                  15      


Asp Gly Ile Ile Lys Ala Gly Pro Ala Val Ala Val Val Gly Gln Ala 
            20                  25                  30          


Thr Gln Ile Ala Lys 
        35          


<210>  19
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cecropin P1

<400>  19

Ser Trp Leu Ser Lys Thr Ala Lys Lys Leu Glu Asn Ser Ala Lys Lys 
1               5                   10                  15      


Arg Ile Ser Glu Gly Ile Ala Ile Ala Ile Gln Gly Gly Pro Arg 
            20                  25                  30      


<210>  20
<211>  59
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CECD

<400>  20

Met Asn Phe Thr Lys Leu Phe Ala Ile Val Leu Leu Ala Ala Leu Val 
1               5                   10                  15      


Leu Leu Gly Gln Thr Glu Ala Gly Gly Leu Lys Lys Leu Gly Lys Lys 
            20                  25                  30          


Leu Glu Gly Ala Gly Lys Arg Val Phe Lys Ala Ser Glu Lys Ala Leu 
        35                  40                  45              


Pro Val Val Val Gly Ile Lys Ala Ile Gly Lys 
    50                  55                  


<210>  21
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Papiliocin

<400>  21

Arg Trp Lys Ile Phe Lys Lys Ile Glu Lys Val Gly Arg Asn Val Arg 
1               5                   10                  15      


Asp Gly Ile Ile Lys Ala Gly Pro Ala Val Ala Val Val Gly Gln Ala 
            20                  25                  30          


Ala Thr Val Val Lys 
        35          


<210>  22
<211>  66
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Moricin

<400>  22

Met Asn Ile Leu Lys Phe Phe Phe Val Phe Ile Val Ala Met Ser Leu 
1               5                   10                  15      


Val Ser Cys Ser Thr Ala Ala Pro Ala Lys Ile Pro Ile Lys Ala Ile 
            20                  25                  30          


Lys Thr Val Gly Lys Ala Val Gly Lys Gly Leu Arg Ala Ile Asn Ile 
        35                  40                  45              


Ala Ser Thr Ala Asn Asp Val Phe Asn Phe Leu Lys Pro Lys Lys Arg 
    50                  55                  60                  


Lys His 
65      


<210>  23
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ceratotoxin A (CtxA)

<400>  23

Ser Ile Gly Ser Ala Leu Lys Lys Ala Leu Pro Val Ala Lys Lys Ile 
1               5                   10                  15      


Gly Lys Ile Ala Leu Pro Ile Ala Lys Ala Ala Leu Pro Val Ala Ala 
            20                  25                  30          


Gly Leu Val Gly 
        35      


<210>  24
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Melittin

<400>  24

Gly Ile Gly Ala Val Leu Lys Val Leu Thr Thr Gly Leu Pro Ala Leu 
1               5                   10                  15      


Ile Ser Trp Ile Lys Arg Lys Arg Gln Gln 
            20                  25      


<210>  25
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magainin 2, Cecropin A

<400>  25

Lys Trp Lys Leu Phe Lys Lys Ile Lys Phe Leu His Ser Ala Lys Lys 
1               5                   10                  15      


Phe Ile Gly Lys Phe Leu His Ser Ala Lys Lys Phe Gly Lys Ala Phe 
            20                  25                  30          


Val Gly Glu Ile Met Asn Ser 
        35                  


<210>  26
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Dermaseptin

<400>  26

Ala Leu Trp Met Thr Leu Leu Lys Lys Val Leu Lys Ala Ala Ala Lys 
1               5                   10                  15      


Ala Leu Asn Ala Val Leu Val Gly Ala Asn Ala 
            20                  25          


<210>  27
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Bombinin-H4

<400>  27

Ile Ile Gly Pro Val Leu Gly Leu Val Gly Ser Ala Leu Gly Gly Leu 
1               5                   10                  15      


Leu Lys Lys Ile Gly 
            20      


<210>  28
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Brevinin-1

<400>  28

Phe Leu Pro Val Leu Ala Gly Ile Ala Ala Lys Val Val Pro Ala Leu 
1               5                   10                  15      


Phe Cys Lys Ile Thr Lys Lys Cys 
            20                  


<210>  29
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Abaecin

<400>  29

Cys Ala Ala Phe Ala Tyr Val Pro Leu Pro Asn Val Pro Gln Pro Gly 
1               5                   10                  15      


Arg Arg Pro Phe Pro Thr Phe Pro Gly Gln Gly Pro Phe Asn Pro Lys 
            20                  25                  30          


Ile 
    


<210>  30
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Indolicidin

<400>  30

Ile Leu Pro Trp Lys Trp Pro Trp Trp Pro Trp Arg Arg 
1               5                   10              


<210>  31
<211>  43
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BACTENECIN 5

<400>  31

Arg Phe Arg Pro Pro Ile Arg Arg Pro Pro Ile Arg Pro Pro Phe Tyr 
1               5                   10                  15      


Pro Pro Phe Arg Pro Pro Ile Arg Pro Pro Ile Phe Pro Pro Ile Arg 
            20                  25                  30          


Pro Pro Phe Arg Pro Pro Leu Gly Pro Phe Pro 
        35                  40              


<210>  32
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heliocin

<400>  32

Gln Arg Phe Ile His Pro Thr Tyr Arg Pro Pro Pro Gln Pro Arg Arg 
1               5                   10                  15      


Pro Val Ile Met Arg Ala 
            20          


<210>  33
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chicken AvBD5

<400>  33

Gly Leu Pro Gln Asp Cys Glu Arg Arg Gly Gly Phe Cys Ser His Lys 
1               5                   10                  15      


Ser Cys Pro Pro Gly Ile Gly Arg Ile Gly Leu Cys Ser Lys Glu Asp 
            20                  25                  30          


Phe Cys Cys Arg Ser Arg Trp Tyr Ser 
        35                  40      


<210>  34
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Andersonin-C1

<400>  34

Thr Ser Arg Cys Ile Phe Tyr Arg Arg Lys Lys Cys Ser 
1               5                   10              


<210>  35
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NRC-20

<400>  35

Gly Phe Leu Gly Ile Leu Phe His Gly Val His His Gly Arg Lys Lys 
1               5                   10                  15      


Ala Leu His Met Asn Ser Glu Arg Arg Ser 
            20                  25      


<210>  36
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NCR247

<400>  36

Arg Asn Gly Cys Ile Val Asp Pro Arg Cys Pro Tyr Gln Gln Cys Arg 
1               5                   10                  15      


Arg Pro Leu Tyr Cys Arg Arg Arg 
            20                  


<210>  37
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mBD-12

<400>  37

Cys Arg Leu Gly Arg Gly Lys Cys Arg Arg Thr Cys Ile Glu Ser Glu 
1               5                   10                  15      


Lys Ile Ala Gly Trp Cys Lys Leu Asn Phe Phe Cys Cys Arg Glu Arg 
            20                  25                  30          


Ile 
    


<210>  38
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mBD-6

<400>  38

Cys Met Ser Tyr Gly Gly Ser Cys Gln Arg Ser Cys Asn Gly Gly Phe 
1               5                   10                  15      


Arg Leu Gly Gly His Cys Gly His Pro Lys Ile Arg Cys Cys Arg Arg 
            20                  25                  30          


Lys 
    


<210>  39
<211>  43
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hg-CATH

<400>  39

Arg Arg Phe Arg Arg Thr Val Gly Leu Ser Lys Phe Phe Arg Lys Ala 
1               5                   10                  15      


Arg Lys Lys Leu Gly Lys Gly Leu Gln Lys Ile Lys Asn Val Leu Arg 
            20                  25                  30          


Lys Tyr Leu Pro Arg Pro Gln Tyr Ala Tyr Ala 
        35                  40              


<210>  40
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tur1B

<400>  40

Arg Arg Ile Pro Phe Trp Pro Pro Asn Trp Pro Gly Pro Trp Leu Pro 
1               5                   10                  15      


Pro Trp Ser Pro Pro Asp Phe Arg Ile Pro Arg Ile Leu Arg Lys Arg 
            20                  25                  30          


<210>  41
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tur1A

<400>  41

Arg Arg Ile Arg Phe Arg Pro Pro Tyr Leu Pro Arg Pro Gly Arg Arg 
1               5                   10                  15      


Pro Arg Phe Pro Pro Pro Phe Pro Ile Pro Arg Ile Pro Arg Ile Pro 
            20                  25                  30          


<210>  42
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tachyplesin I

<400>  42

Lys Trp Cys Phe Arg Val Cys Tyr Arg Gly Ile Cys Tyr Arg Arg Cys 
1               5                   10                  15      


Arg 
    


<210>  43
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NEMURI

<400>  43

Asp Ala Arg Ala Arg Arg Ile Val Arg Ala Gly Arg Arg Arg Gly Gly 
1               5                   10                  15      


Arg Arg Gly Gly Arg Arg Gly Gly Arg Arg Ser Ala Arg Lys Ser 
            20                  25                  30      


<210>  44
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ModoCath6

<400>  44

Val Arg Arg Ser Lys Arg Gly Ile Lys Val Pro Ser Phe Val Lys Lys 
1               5                   10                  15      


Val Leu Lys Asp Val Val Ser Glu Ser Ile Ser 
            20                  25          


<210>  45
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Myticalin C6

<400>  45

Arg Arg Arg Arg Arg Phe Arg Arg Val Ile Arg Arg Ile Arg Leu Pro 
1               5                   10                  15      


Lys Tyr Leu Thr Ile Asn Thr Glu 
            20                  


<210>  46
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCRAMP

<400>  46

Gly Leu Leu Arg Lys Gly Gly Glu Lys Ile Gly Glu Lys Leu Lys Lys 
1               5                   10                  15      


Ile Gly Gln Lys Ile Lys Asn Phe Phe Gln Lys Leu Val Pro Gln Pro 
            20                  25                  30          


Glu Gln 
        


<210>  47
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment 1 of LL37FRRV

<400>  47

Cys Leu Leu Gly Asp Phe Phe Arg 
1               5               


<210>  48
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment 2 of LL37FRRV

<400>  48

Arg Val Lys Glu Lys Ile Gly 
1               5           


<210>  49
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GXRRXGC


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  G is acetylated

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is any amino acid except Cysteine.

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is any amino acid except Cysteine.

<400>  49

Gly Xaa Arg Arg Xaa Gly Cys 
1               5           


<210>  50
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LL37FRRV with acetyl cap


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Acetyl cap

<400>  50

Cys Leu Leu Gly Asp Phe Phe Arg Arg Val Lys Glu Lys Ile Gly 
1               5                   10                  15  


<210>  51
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LL37FRRV with 1 Ala substitution and acetyl cap


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Acetyl cap

<400>  51

Cys Leu Leu Gly Asp Phe Phe Arg Arg Val Lys Glu Ala Ile Gly 
1               5                   10                  15  


<210>  52
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LL37FRRV with 2 Ala substitutions and acetyl cap


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Acetyl cap

<400>  52

Cys Leu Leu Gly Asp Phe Phe Arg Arg Val Ala Glu Ala Ile Gly 
1               5                   10                  15  


<210>  53
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LL37FRRV with 1 Ala substitution

<400>  53

Cys Leu Leu Gly Asp Phe Phe Arg Arg Val Lys Glu Ala Ile Gly 
1               5                   10                  15  


<210>  54
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LL37FRRV with 2 Ala substitutions

<400>  54

Cys Leu Leu Gly Asp Phe Phe Arg Arg Val Ala Glu Ala Ile Gly 
1               5                   10                  15  


