               SEQUENCE LISTING

<110> Bayer Aktiengesellschaft, The Broad Institute, Inc., Dana-Farber Cancer Institute, Inc.

<120> 4H-Pyrrolo[3,2-c]pyridin-4-one Compounds

<130> BHC 18 3 052 

<160> 7

<170> BiSSAP 1.3.6

<210> 1
<211> 1210
<212> PRT
<213> human


<220> 
<223> human EGFR, UniProt Accession No. P00533-1

<400> 1
Met Arg Pro Ser Gly Thr Ala Gly Ala Ala Leu Leu Ala Leu Leu Ala 
1               5                   10                  15      
Ala Leu Cys Pro Ala Ser Arg Ala Leu Glu Glu Lys Lys Val Cys Gln 
            20                  25                  30          
Gly Thr Ser Asn Lys Leu Thr Gln Leu Gly Thr Phe Glu Asp His Phe 
        35                  40                  45              
Leu Ser Leu Gln Arg Met Phe Asn Asn Cys Glu Val Val Leu Gly Asn 
    50                  55                  60                  
Leu Glu Ile Thr Tyr Val Gln Arg Asn Tyr Asp Leu Ser Phe Leu Lys 
65                  70                  75                  80  
Thr Ile Gln Glu Val Ala Gly Tyr Val Leu Ile Ala Leu Asn Thr Val 
                85                  90                  95      
Glu Arg Ile Pro Leu Glu Asn Leu Gln Ile Ile Arg Gly Asn Met Tyr 
            100                 105                 110         
Tyr Glu Asn Ser Tyr Ala Leu Ala Val Leu Ser Asn Tyr Asp Ala Asn 
        115                 120                 125             
Lys Thr Gly Leu Lys Glu Leu Pro Met Arg Asn Leu Gln Glu Ile Leu 
    130                 135                 140                 
His Gly Ala Val Arg Phe Ser Asn Asn Pro Ala Leu Cys Asn Val Glu 
145                 150                 155                 160 
Ser Ile Gln Trp Arg Asp Ile Val Ser Ser Asp Phe Leu Ser Asn Met 
                165                 170                 175     
Ser Met Asp Phe Gln Asn His Leu Gly Ser Cys Gln Lys Cys Asp Pro 
            180                 185                 190         
Ser Cys Pro Asn Gly Ser Cys Trp Gly Ala Gly Glu Glu Asn Cys Gln 
        195                 200                 205             
Lys Leu Thr Lys Ile Ile Cys Ala Gln Gln Cys Ser Gly Arg Cys Arg 
    210                 215                 220                 
Gly Lys Ser Pro Ser Asp Cys Cys His Asn Gln Cys Ala Ala Gly Cys 
225                 230                 235                 240 
Thr Gly Pro Arg Glu Ser Asp Cys Leu Val Cys Arg Lys Phe Arg Asp 
                245                 250                 255     
Glu Ala Thr Cys Lys Asp Thr Cys Pro Pro Leu Met Leu Tyr Asn Pro 
            260                 265                 270         
Thr Thr Tyr Gln Met Asp Val Asn Pro Glu Gly Lys Tyr Ser Phe Gly 
        275                 280                 285             
Ala Thr Cys Val Lys Lys Cys Pro Arg Asn Tyr Val Val Thr Asp His 
    290                 295                 300                 
Gly Ser Cys Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu 
305                 310                 315                 320 
Asp Gly Val Arg Lys Cys Lys Lys Cys Glu Gly Pro Cys Arg Lys Val 
                325                 330                 335     
Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn 
            340                 345                 350         
Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp 
        355                 360                 365             
Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr 
    370                 375                 380                 
Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu 
385                 390                 395                 400 
Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp 
                405                 410                 415     
Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln 
            420                 425                 430         
His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu 
        435                 440                 445             
Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser 
    450                 455                 460                 
Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu 
465                 470                 475                 480 
Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu 
                485                 490                 495     
Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro 
            500                 505                 510         
Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn 
        515                 520                 525             
Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly 
    530                 535                 540                 
Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro 
545                 550                 555                 560 
Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro 
                565                 570                 575     
Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val 
            580                 585                 590         
Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp 
        595                 600                 605             
Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His Pro Asn Cys 
    610                 615                 620                 
Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly 
625                 630                 635                 640 
Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu 
                645                 650                 655     
Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met Arg Arg Arg His 
            660                 665                 670         
Ile Val Arg Lys Arg Thr Leu Arg Arg Leu Leu Gln Glu Arg Glu Leu 
        675                 680                 685             
Val Glu Pro Leu Thr Pro Ser Gly Glu Ala Pro Asn Gln Ala Leu Leu 
    690                 695                 700                 
Arg Ile Leu Lys Glu Thr Glu Phe Lys Lys Ile Lys Val Leu Gly Ser 
705                 710                 715                 720 
Gly Ala Phe Gly Thr Val Tyr Lys Gly Leu Trp Ile Pro Glu Gly Glu 
                725                 730                 735     
Lys Val Lys Ile Pro Val Ala Ile Lys Glu Leu Arg Glu Ala Thr Ser 
            740                 745                 750         
Pro Lys Ala Asn Lys Glu Ile Leu Asp Glu Ala Tyr Val Met Ala Ser 
        755                 760                 765             
Val Asp Asn Pro His Val Cys Arg Leu Leu Gly Ile Cys Leu Thr Ser 
    770                 775                 780                 
Thr Val Gln Leu Ile Thr Gln Leu Met Pro Phe Gly Cys Leu Leu Asp 
785                 790                 795                 800 
Tyr Val Arg Glu His Lys Asp Asn Ile Gly Ser Gln Tyr Leu Leu Asn 
                805                 810                 815     
Trp Cys Val Gln Ile Ala Lys Gly Met Asn Tyr Leu Glu Asp Arg Arg 
            820                 825                 830         
Leu Val His Arg Asp Leu Ala Ala Arg Asn Val Leu Val Lys Thr Pro 
        835                 840                 845             
Gln His Val Lys Ile Thr Asp Phe Gly Leu Ala Lys Leu Leu Gly Ala 
    850                 855                 860                 
Glu Glu Lys Glu Tyr His Ala Glu Gly Gly Lys Val Pro Ile Lys Trp 
865                 870                 875                 880 
Met Ala Leu Glu Ser Ile Leu His Arg Ile Tyr Thr His Gln Ser Asp 
                885                 890                 895     
Val Trp Ser Tyr Gly Val Thr Val Trp Glu Leu Met Thr Phe Gly Ser 
            900                 905                 910         
Lys Pro Tyr Asp Gly Ile Pro Ala Ser Glu Ile Ser Ser Ile Leu Glu 
        915                 920                 925             
Lys Gly Glu Arg Leu Pro Gln Pro Pro Ile Cys Thr Ile Asp Val Tyr 
    930                 935                 940                 
Met Ile Met Val Lys Cys Trp Met Ile Asp Ala Asp Ser Arg Pro Lys 
945                 950                 955                 960 
Phe Arg Glu Leu Ile Ile Glu Phe Ser Lys Met Ala Arg Asp Pro Gln 
                965                 970                 975     
Arg Tyr Leu Val Ile Gln Gly Asp Glu Arg Met His Leu Pro Ser Pro 
            980                 985                 990         
Thr Asp Ser Asn Phe Tyr Arg Ala Leu Met Asp Glu Glu Asp Met Asp 
        995                 1000                1005            
Asp Val Val Asp Ala Asp Glu Tyr Leu Ile Pro Gln Gln Gly Phe Phe 
    1010                1015                1020                
Ser Ser Pro Ser Thr Ser Arg Thr Pro Leu Leu Ser Ser Leu Ser Ala 
1025                1030                1035                1040
Thr Ser Asn Asn Ser Thr Val Ala Cys Ile Asp Arg Asn Gly Leu Gln 
                1045                1050                1055    
Ser Cys Pro Ile Lys Glu Asp Ser Phe Leu Gln Arg Tyr Ser Ser Asp 
            1060                1065                1070        
Pro Thr Gly Ala Leu Thr Glu Asp Ser Ile Asp Asp Thr Phe Leu Pro 
        1075                1080                1085            
Val Pro Glu Tyr Ile Asn Gln Ser Val Pro Lys Arg Pro Ala Gly Ser 
    1090                1095                1100                
Val Gln Asn Pro Val Tyr His Asn Gln Pro Leu Asn Pro Ala Pro Ser 
1105                1110                1115                1120
Arg Asp Pro His Tyr Gln Asp Pro His Ser Thr Ala Val Gly Asn Pro 
                1125                1130                1135    
Glu Tyr Leu Asn Thr Val Gln Pro Thr Cys Val Asn Ser Thr Phe Asp 
            1140                1145                1150        
Ser Pro Ala His Trp Ala Gln Lys Gly Ser His Gln Ile Ser Leu Asp 
        1155                1160                1165            
Asn Pro Asp Tyr Gln Gln Asp Phe Phe Pro Lys Glu Ala Lys Pro Asn 
    1170                1175                1180                
Gly Ile Phe Lys Gly Ser Thr Ala Glu Asn Ala Glu Tyr Leu Arg Val 
1185                1190                1195                1200
Ala Pro Gln Ser Ser Glu Phe Ile Gly Ala 
                1205                1210

<210> 2
<211> 3859
<212> RNA
<213> human


<220> 
<223> human EGFR, NCBI Reference Sequence: NM_001346897.1

<400> 2
gtccgggcag cccccggcgc agcgcggccg cagcagcctc cgccccccgc acggtgtgag       60

cgcccgacgc ggccgaggcg gccggagtcc cgagctagcc ccggcggccg ccgccgccca      120

gaccggacga caggccacct cgtcggcgtc cgcccgagtc cccgcctcgc cgccaacgcc      180

acaaccaccg cgcacggccc cctgactccg tccagtattg atcgggagag ccggagcgag      240

ctcttcgggg agcagcgatg cgaccctccg ggacggccgg ggcagcgctc ctggcgctgc      300

tggctgcgct ctgcccggcg agtcgggctc tggaggaaaa gaaagtttgc caaggcacga      360

gtaacaagct cacgcagttg ggcacttttg aagatcattt tctcagcctc cagaggatgt      420

tcaataactg tgaggtggtc cttgggaatt tggaaattac ctatgtgcag aggaattatg      480

atctttcctt cttaaagacc atccaggagg tggctggtta tgtcctcatt gccctcaaca      540

cagtggagcg aattcctttg gaaaacctgc agatcatcag aggaaatatg tactacgaaa      600

attcctatgc cttagcagtc ttatctaact atgatgcaaa taaaaccgga ctgaaggagc      660

tgcccatgag aaatttacag ggccaaaagt gtgatccaag ctgtcccaat gggagctgct      720

ggggtgcagg agaggagaac tgccagaaac tgaccaaaat catctgtgcc cagcagtgct      780

ccgggcgctg ccgtggcaag tcccccagtg actgctgcca caaccagtgt gctgcaggct      840

gcacaggccc ccgggagagc gactgcctgg tctgccgcaa attccgagac gaagccacgt      900

gcaaggacac ctgcccccca ctcatgctct acaaccccac cacgtaccag atggatgtga      960

accccgaggg caaatacagc tttggtgcca cctgcgtgaa gaagtgtccc cgtaattatg     1020

tggtgacaga tcacggctcg tgcgtccgag cctgtggggc cgacagctat gagatggagg     1080

aagacggcgt ccgcaagtgt aagaagtgcg aagggccttg ccgcaaagtg tgtaacggaa     1140

taggtattgg tgaatttaaa gactcactct ccataaatgc tacgaatatt aaacacttca     1200

aaaactgcac ctccatcagt ggcgatctcc acatcctgcc ggtggcattt aggggtgact     1260

ccttcacaca tactcctcct ctggatccac aggaactgga tattctgaaa accgtaaagg     1320

aaatcacagg gtttttgctg attcaggctt ggcctgaaaa caggacggac ctccatgcct     1380

ttgagaacct agaaatcata cgcggcagga ccaagcaaca tggtcagttt tctcttgcag     1440

tcgtcagcct gaacataaca tccttgggat tacgctccct caaggagata agtgatggag     1500

atgtgataat ttcaggaaac aaaaatttgt gctatgcaaa tacaataaac tggaaaaaac     1560

tgtttgggac ctccggtcag aaaaccaaaa ttataagcaa cagaggtgaa aacagctgca     1620

aggccacagg ccaggtctgc catgccttgt gctcccccga gggctgctgg ggcccggagc     1680

ccagggactg cgtctcttgc cggaatgtca gccgaggcag ggaatgcgtg gacaagtgca     1740

accttctgga gggtgagcca agggagtttg tggagaactc tgagtgcata cagtgccacc     1800

cagagtgcct gcctcaggcc atgaacatca cctgcacagg acggggacca gacaactgta     1860

tccagtgtgc ccactacatt gacggccccc actgcgtcaa gacctgcccg gcaggagtca     1920

tgggagaaaa caacaccctg gtctggaagt acgcagacgc cggccatgtg tgccacctgt     1980

gccatccaaa ctgcacctac ggatgcactg ggccaggtct tgaaggctgt ccaacgaatg     2040

ggcctaagat cccgtccatc gccactggga tggtgggggc cctcctcttg ctgctggtgg     2100

tggccctggg gatcggcctc ttcatgcgaa ggcgccacat cgttcggaag cgcacgctgc     2160

ggaggctgct gcaggagagg gagcttgtgg agcctcttac acccagtgga gaagctccca     2220

accaagctct cttgaggatc ttgaaggaaa ctgaattcaa aaagatcaaa gtgctgggct     2280

ccggtgcgtt cggcacggtg tataagggac tctggatccc agaaggtgag aaagttaaaa     2340

ttcccgtcgc tatcaaggaa ttaagagaag caacatctcc gaaagccaac aaggaaatcc     2400

tcgatgaagc ctacgtgatg gccagcgtgg acaaccccca cgtgtgccgc ctgctgggca     2460

tctgcctcac ctccaccgtg cagctcatca cgcagctcat gcccttcggc tgcctcctgg     2520

actatgtccg ggaacacaaa gacaatattg gctcccagta cctgctcaac tggtgtgtgc     2580

agatcgcaaa gggcatgaac tacttggagg accgtcgctt ggtgcaccgc gacctggcag     2640

ccaggaacgt actggtgaaa acaccgcagc atgtcaagat cacagatttt gggctggcca     2700

aactgctggg tgcggaagag aaagaatacc atgcagaagg aggcaaagtg cctatcaagt     2760

ggatggcatt ggaatcaatt ttacacagaa tctataccca ccagagtgat gtctggagct     2820

acggggtgac tgtttgggag ttgatgacct ttggatccaa gccatatgac ggaatccctg     2880

ccagcgagat ctcctccatc ctggagaaag gagaacgcct ccctcagcca cccatatgta     2940

ccatcgatgt ctacatgatc atggtcaagt gctggatgat agacgcagat agtcgcccaa     3000

agttccgtga gttgatcatc gaattctcca aaatggcccg agacccccag cgctaccttg     3060

tcattcaggg ggatgaaaga atgcatttgc caagtcctac agactccaac ttctaccgtg     3120

ccctgatgga tgaagaagac atggacgacg tggtggatgc cgacgagtac ctcatcccac     3180

agcagggctt cttcagcagc ccctccacgt cacggactcc cctcctgagc tctctgagtg     3240

caaccagcaa caattccacc gtggcttgca ttgatagaaa tgggctgcaa agctgtccca     3300

tcaaggaaga cagcttcttg cagcgataca gctcagaccc cacaggcgcc ttgactgagg     3360

acagcataga cgacaccttc ctcccagtgc ctggtgagtg gcttgtctgg aaacagtcct     3420

gctcctcaac ctcctcgacc cactcagcag cagccagtct ccagtgtcca agccaggtgc     3480

tccctccagc atctccagag ggggaaacag tggcagattt gcagacacag tgaagggcgt     3540

aaggagcaga taaacacatg accgagcctg cacaagctct ttgttgtgtc tggttgtttg     3600

ctgtacctct gttgtaagaa tgaatctgca aaatttctag cttatgaagc aaatcacgga     3660

catacacatc tgtgtgtgtg agtgttcatg atgtgtgtac atctgtgtat gtgtgtgtgt     3720

gtatgtgtgt gtttgtgaca gatttgatcc ctgttctctc tgctggctct atcttgacct     3780

gtgaaacgta tatttaacta attaaatatt agttaatatt aataaatttt aagctttatc     3840

cagaaaaaaa aaaaaaaaa                                                  3859


<210> 3
<211> 788
<212> PRT
<213> human


<220> 
<223> GST-EGFR (Wild Type)

<400> 3
Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 
1               5                   10                  15      
Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 
            20                  25                  30          
Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 
        35                  40                  45              
Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys 
    50                  55                  60                  
Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn 
65                  70                  75                  80  
Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu 
                85                  90                  95      
Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser 
            100                 105                 110         
Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu 
        115                 120                 125             
Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn 
    130                 135                 140                 
Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp 
145                 150                 155                 160 
Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu 
                165                 170                 175     
Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr 
            180                 185                 190         
Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala 
        195                 200                 205             
Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Pro Ile Thr Ser 
    210                 215                 220                 
Leu Tyr Lys Lys Ala Gly Ser Asp Tyr Asp Ile Pro Thr Thr Thr Glu 
225                 230                 235                 240 
Asn Leu Tyr Phe Gln Gly Arg Arg Arg His Ile Val Arg Lys Arg Thr 
                245                 250                 255     
Leu Arg Arg Leu Leu Gln Glu Arg Glu Leu Val Glu Pro Leu Thr Pro 
            260                 265                 270         
Ser Gly Glu Ala Pro Asn Gln Ala Leu Leu Arg Ile Leu Lys Glu Thr 
        275                 280                 285             
Glu Phe Lys Lys Ile Lys Val Leu Gly Ser Gly Ala Phe Gly Thr Val 
    290                 295                 300                 
Tyr Lys Gly Leu Trp Ile Pro Glu Gly Glu Lys Val Lys Ile Pro Val 
305                 310                 315                 320 
Ala Ile Lys Glu Leu Arg Glu Ala Thr Ser Pro Lys Ala Asn Lys Glu 
                325                 330                 335     
Ile Leu Asp Glu Ala Tyr Val Met Ala Ser Val Asp Asn Pro His Val 
            340                 345                 350         
Cys Arg Leu Leu Gly Ile Cys Leu Thr Ser Thr Val Gln Leu Ile Thr 
        355                 360                 365             
Gln Leu Met Pro Phe Gly Cys Leu Leu Asp Tyr Val Arg Glu His Lys 
    370                 375                 380                 
Asp Asn Ile Gly Ser Gln Tyr Leu Leu Asn Trp Cys Val Gln Ile Ala 
385                 390                 395                 400 
Lys Gly Met Asn Tyr Leu Glu Asp Arg Arg Leu Val His Arg Asp Leu 
                405                 410                 415     
Ala Ala Arg Asn Val Leu Val Lys Thr Pro Gln His Val Lys Ile Thr 
            420                 425                 430         
Asp Phe Gly Leu Ala Lys Leu Leu Gly Ala Glu Glu Lys Glu Tyr His 
        435                 440                 445             
Ala Glu Gly Gly Lys Val Pro Ile Lys Trp Met Ala Leu Glu Ser Ile 
    450                 455                 460                 
Leu His Arg Ile Tyr Thr His Gln Ser Asp Val Trp Ser Tyr Gly Val 
465                 470                 475                 480 
Thr Val Trp Glu Leu Met Thr Phe Gly Ser Lys Pro Tyr Asp Gly Ile 
                485                 490                 495     
Pro Ala Ser Glu Ile Ser Ser Ile Leu Glu Lys Gly Glu Arg Leu Pro 
            500                 505                 510         
Gln Pro Pro Ile Cys Thr Ile Asp Val Tyr Met Ile Met Val Lys Cys 
        515                 520                 525             
Trp Met Ile Asp Ala Asp Ser Arg Pro Lys Phe Arg Glu Leu Ile Ile 
    530                 535                 540                 
Glu Phe Ser Lys Met Ala Arg Asp Pro Gln Arg Tyr Leu Val Ile Gln 
545                 550                 555                 560 
Gly Asp Glu Arg Met His Leu Pro Ser Pro Thr Asp Ser Asn Phe Tyr 
                565                 570                 575     
Arg Ala Leu Met Asp Glu Glu Asp Met Asp Asp Val Val Asp Ala Asp 
            580                 585                 590         
Glu Tyr Leu Ile Pro Gln Gln Gly Phe Phe Ser Ser Pro Ser Thr Ser 
        595                 600                 605             
Arg Thr Pro Leu Leu Ser Ser Leu Ser Ala Thr Ser Asn Asn Ser Thr 
    610                 615                 620                 
Val Ala Cys Ile Asp Arg Asn Gly Leu Gln Ser Cys Pro Ile Lys Glu 
625                 630                 635                 640 
Asp Ser Phe Leu Gln Arg Tyr Ser Ser Asp Pro Thr Gly Ala Leu Thr 
                645                 650                 655     
Glu Asp Ser Ile Asp Asp Thr Phe Leu Pro Val Pro Glu Tyr Ile Asn 
            660                 665                 670         
Gln Ser Val Pro Lys Arg Pro Ala Gly Ser Val Gln Asn Pro Val Tyr 
        675                 680                 685             
His Asn Gln Pro Leu Asn Pro Ala Pro Ser Arg Asp Pro His Tyr Gln 
    690                 695                 700                 
Asp Pro His Ser Thr Ala Val Gly Asn Pro Glu Tyr Leu Asn Thr Val 
705                 710                 715                 720 
Gln Pro Thr Cys Val Asn Ser Thr Phe Asp Ser Pro Ala His Trp Ala 
                725                 730                 735     
Gln Lys Gly Ser His Gln Ile Ser Leu Asp Asn Pro Asp Tyr Gln Gln 
            740                 745                 750         
Asp Phe Phe Pro Lys Glu Ala Lys Pro Asn Gly Ile Phe Lys Gly Ser 
        755                 760                 765             
Thr Ala Glu Asn Ala Glu Tyr Leu Arg Val Ala Pro Gln Ser Ser Glu 
    770                 775                 780                 
Phe Ile Gly Ala 
785             

<210> 4
<211> 791
<212> PRT
<213> human


<220> 
<223> GST-EGFR (ASV between V769 and D770)

<400> 4
Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 
1               5                   10                  15      
Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 
            20                  25                  30          
Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 
        35                  40                  45              
Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys 
    50                  55                  60                  
Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn 
65                  70                  75                  80  
Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu 
                85                  90                  95      
Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser 
            100                 105                 110         
Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu 
        115                 120                 125             
Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn 
    130                 135                 140                 
Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp 
145                 150                 155                 160 
Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu 
                165                 170                 175     
Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr 
            180                 185                 190         
Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala 
        195                 200                 205             
Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Pro Ile Thr Ser 
    210                 215                 220                 
Leu Tyr Lys Lys Ala Gly Ser Asp Tyr Asp Ile Pro Thr Thr Thr Glu 
225                 230                 235                 240 
Asn Leu Tyr Phe Gln Gly Arg Arg Arg His Ile Val Arg Lys Arg Thr 
                245                 250                 255     
Leu Arg Arg Leu Leu Gln Glu Arg Glu Leu Val Glu Pro Leu Thr Pro 
            260                 265                 270         
Ser Gly Glu Ala Pro Asn Gln Ala Leu Leu Arg Ile Leu Lys Glu Thr 
        275                 280                 285             
Glu Phe Lys Lys Ile Lys Val Leu Gly Ser Gly Ala Phe Gly Thr Val 
    290                 295                 300                 
Tyr Lys Gly Leu Trp Ile Pro Glu Gly Glu Lys Val Lys Ile Pro Val 
305                 310                 315                 320 
Ala Ile Lys Glu Leu Arg Glu Ala Thr Ser Pro Lys Ala Asn Lys Glu 
                325                 330                 335     
Ile Leu Asp Glu Ala Tyr Val Met Ala Ser Val Ala Ser Val Asp Asn 
            340                 345                 350         
Pro His Val Cys Arg Leu Leu Gly Ile Cys Leu Thr Ser Thr Val Gln 
        355                 360                 365             
Leu Ile Thr Gln Leu Met Pro Phe Gly Cys Leu Leu Asp Tyr Val Arg 
    370                 375                 380                 
Glu His Lys Asp Asn Ile Gly Ser Gln Tyr Leu Leu Asn Trp Cys Val 
385                 390                 395                 400 
Gln Ile Ala Lys Gly Met Asn Tyr Leu Glu Asp Arg Arg Leu Val His 
                405                 410                 415     
Arg Asp Leu Ala Ala Arg Asn Val Leu Val Lys Thr Pro Gln His Val 
            420                 425                 430         
Lys Ile Thr Asp Phe Gly Leu Ala Lys Leu Leu Gly Ala Glu Glu Lys 
        435                 440                 445             
Glu Tyr His Ala Glu Gly Gly Lys Val Pro Ile Lys Trp Met Ala Leu 
    450                 455                 460                 
Glu Ser Ile Leu His Arg Ile Tyr Thr His Gln Ser Asp Val Trp Ser 
465                 470                 475                 480 
Tyr Gly Val Thr Val Trp Glu Leu Met Thr Phe Gly Ser Lys Pro Tyr 
                485                 490                 495     
Asp Gly Ile Pro Ala Ser Glu Ile Ser Ser Ile Leu Glu Lys Gly Glu 
            500                 505                 510         
Arg Leu Pro Gln Pro Pro Ile Cys Thr Ile Asp Val Tyr Met Ile Met 
        515                 520                 525             
Val Lys Cys Trp Met Ile Asp Ala Asp Ser Arg Pro Lys Phe Arg Glu 
    530                 535                 540                 
Leu Ile Ile Glu Phe Ser Lys Met Ala Arg Asp Pro Gln Arg Tyr Leu 
545                 550                 555                 560 
Val Ile Gln Gly Asp Glu Arg Met His Leu Pro Ser Pro Thr Asp Ser 
                565                 570                 575     
Asn Phe Tyr Arg Ala Leu Met Asp Glu Glu Asp Met Asp Asp Val Val 
            580                 585                 590         
Asp Ala Asp Glu Tyr Leu Ile Pro Gln Gln Gly Phe Phe Ser Ser Pro 
        595                 600                 605             
Ser Thr Ser Arg Thr Pro Leu Leu Ser Ser Leu Ser Ala Thr Ser Asn 
    610                 615                 620                 
Asn Ser Thr Val Ala Cys Ile Asp Arg Asn Gly Leu Gln Ser Cys Pro 
625                 630                 635                 640 
Ile Lys Glu Asp Ser Phe Leu Gln Arg Tyr Ser Ser Asp Pro Thr Gly 
                645                 650                 655     
Ala Leu Thr Glu Asp Ser Ile Asp Asp Thr Phe Leu Pro Val Pro Glu 
            660                 665                 670         
Tyr Ile Asn Gln Ser Val Pro Lys Arg Pro Ala Gly Ser Val Gln Asn 
        675                 680                 685             
Pro Val Tyr His Asn Gln Pro Leu Asn Pro Ala Pro Ser Arg Asp Pro 
    690                 695                 700                 
His Tyr Gln Asp Pro His Ser Thr Ala Val Gly Asn Pro Glu Tyr Leu 
705                 710                 715                 720 
Asn Thr Val Gln Pro Thr Cys Val Asn Ser Thr Phe Asp Ser Pro Ala 
                725                 730                 735     
His Trp Ala Gln Lys Gly Ser His Gln Ile Ser Leu Asp Asn Pro Asp 
            740                 745                 750         
Tyr Gln Gln Asp Phe Phe Pro Lys Glu Ala Lys Pro Asn Gly Ile Phe 
        755                 760                 765             
Lys Gly Ser Thr Ala Glu Asn Ala Glu Tyr Leu Arg Val Ala Pro Gln 
    770                 775                 780                 
Ser Ser Glu Phe Ile Gly Ala 
785                 790     

<210> 5
<211> 791
<212> PRT
<213> human


<220> 
<223> GST-EGFR (SVD between D770 and N771)

<400> 5
Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 
1               5                   10                  15      
Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 
            20                  25                  30          
Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 
        35                  40                  45              
Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys 
    50                  55                  60                  
Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn 
65                  70                  75                  80  
Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu 
                85                  90                  95      
Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser 
            100                 105                 110         
Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu 
        115                 120                 125             
Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn 
    130                 135                 140                 
Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp 
145                 150                 155                 160 
Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu 
                165                 170                 175     
Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr 
            180                 185                 190         
Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala 
        195                 200                 205             
Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Pro Ile Thr Ser 
    210                 215                 220                 
Leu Tyr Lys Lys Ala Gly Ser Asp Tyr Asp Ile Pro Thr Thr Thr Glu 
225                 230                 235                 240 
Asn Leu Tyr Phe Gln Gly Arg Arg Arg His Ile Val Arg Lys Arg Thr 
                245                 250                 255     
Leu Arg Arg Leu Leu Gln Glu Arg Glu Leu Val Glu Pro Leu Thr Pro 
            260                 265                 270         
Ser Gly Glu Ala Pro Asn Gln Ala Leu Leu Arg Ile Leu Lys Glu Thr 
        275                 280                 285             
Glu Phe Lys Lys Ile Lys Val Leu Gly Ser Gly Ala Phe Gly Thr Val 
    290                 295                 300                 
Tyr Lys Gly Leu Trp Ile Pro Glu Gly Glu Lys Val Lys Ile Pro Val 
305                 310                 315                 320 
Ala Ile Lys Glu Leu Arg Glu Ala Thr Ser Pro Lys Ala Asn Lys Glu 
                325                 330                 335     
Ile Leu Asp Glu Ala Tyr Val Met Ala Ser Val Asp Ser Val Asp Asn 
            340                 345                 350         
Pro His Val Cys Arg Leu Leu Gly Ile Cys Leu Thr Ser Thr Val Gln 
        355                 360                 365             
Leu Ile Thr Gln Leu Met Pro Phe Gly Cys Leu Leu Asp Tyr Val Arg 
    370                 375                 380                 
Glu His Lys Asp Asn Ile Gly Ser Gln Tyr Leu Leu Asn Trp Cys Val 
385                 390                 395                 400 
Gln Ile Ala Lys Gly Met Asn Tyr Leu Glu Asp Arg Arg Leu Val His 
                405                 410                 415     
Arg Asp Leu Ala Ala Arg Asn Val Leu Val Lys Thr Pro Gln His Val 
            420                 425                 430         
Lys Ile Thr Asp Phe Gly Leu Ala Lys Leu Leu Gly Ala Glu Glu Lys 
        435                 440                 445             
Glu Tyr His Ala Glu Gly Gly Lys Val Pro Ile Lys Trp Met Ala Leu 
    450                 455                 460                 
Glu Ser Ile Leu His Arg Ile Tyr Thr His Gln Ser Asp Val Trp Ser 
465                 470                 475                 480 
Tyr Gly Val Thr Val Trp Glu Leu Met Thr Phe Gly Ser Lys Pro Tyr 
                485                 490                 495     
Asp Gly Ile Pro Ala Ser Glu Ile Ser Ser Ile Leu Glu Lys Gly Glu 
            500                 505                 510         
Arg Leu Pro Gln Pro Pro Ile Cys Thr Ile Asp Val Tyr Met Ile Met 
        515                 520                 525             
Val Lys Cys Trp Met Ile Asp Ala Asp Ser Arg Pro Lys Phe Arg Glu 
    530                 535                 540                 
Leu Ile Ile Glu Phe Ser Lys Met Ala Arg Asp Pro Gln Arg Tyr Leu 
545                 550                 555                 560 
Val Ile Gln Gly Asp Glu Arg Met His Leu Pro Ser Pro Thr Asp Ser 
                565                 570                 575     
Asn Phe Tyr Arg Ala Leu Met Asp Glu Glu Asp Met Asp Asp Val Val 
            580                 585                 590         
Asp Ala Asp Glu Tyr Leu Ile Pro Gln Gln Gly Phe Phe Ser Ser Pro 
        595                 600                 605             
Ser Thr Ser Arg Thr Pro Leu Leu Ser Ser Leu Ser Ala Thr Ser Asn 
    610                 615                 620                 
Asn Ser Thr Val Ala Cys Ile Asp Arg Asn Gly Leu Gln Ser Cys Pro 
625                 630                 635                 640 
Ile Lys Glu Asp Ser Phe Leu Gln Arg Tyr Ser Ser Asp Pro Thr Gly 
                645                 650                 655     
Ala Leu Thr Glu Asp Ser Ile Asp Asp Thr Phe Leu Pro Val Pro Glu 
            660                 665                 670         
Tyr Ile Asn Gln Ser Val Pro Lys Arg Pro Ala Gly Ser Val Gln Asn 
        675                 680                 685             
Pro Val Tyr His Asn Gln Pro Leu Asn Pro Ala Pro Ser Arg Asp Pro 
    690                 695                 700                 
His Tyr Gln Asp Pro His Ser Thr Ala Val Gly Asn Pro Glu Tyr Leu 
705                 710                 715                 720 
Asn Thr Val Gln Pro Thr Cys Val Asn Ser Thr Phe Asp Ser Pro Ala 
                725                 730                 735     
His Trp Ala Gln Lys Gly Ser His Gln Ile Ser Leu Asp Asn Pro Asp 
            740                 745                 750         
Tyr Gln Gln Asp Phe Phe Pro Lys Glu Ala Lys Pro Asn Gly Ile Phe 
        755                 760                 765             
Lys Gly Ser Thr Ala Glu Asn Ala Glu Tyr Leu Arg Val Ala Pro Gln 
    770                 775                 780                 
Ser Ser Glu Phe Ile Gly Ala 
785                 790     

<210> 6
<211> 14
<212> PRT
<213> sythetic organism


<220> 
<223> biotinylated peptide biotin-Ahx, (C-terminus in amide form)

<400> 6
Ala Glu Glu Glu Glu Tyr Phe Glu Leu Val Ala Lys Lys Lys 
1               5                   10                  

<210> 7
<211> 12
<212> PRT
<213> sythetic organism


<220> 
<223> biotinylated peptide biotin-Ahx, C-terminus in amid form

<400> 7
Val Leu Leu Pro Lys Lys Ser Phe Ala Glu Pro Gly 
1               5                   10          

