               SEQUENCE LISTING

<110> ETH Zurich

<120> Method for labeling a protein

<130> A22714WO

<150> EP19170647.2
<151> 2019-04-23

<160> 45

<170> BiSSAP 1.3.6

<210> 1
<211> 158
<212> PRT
<213> Homo sapiens


<220> 
<223> wild type

<400> 1
Met Ser Gly Ile Ala Leu Ser Arg Leu Ala Gln Glu Arg Lys Ala Trp 
1               5                   10                  15      
Arg Lys Asp His Pro Phe Gly Phe Val Ala Val Pro Thr Lys Asn Pro 
            20                  25                  30          
Asp Gly Thr Met Asn Leu Met Asn Trp Glu Cys Ala Ile Pro Gly Lys 
        35                  40                  45              
Lys Gly Thr Pro Trp Glu Gly Gly Leu Phe Lys Leu Arg Met Leu Phe 
    50                  55                  60                  
Lys Asp Asp Tyr Pro Ser Ser Pro Pro Lys Cys Lys Phe Glu Pro Pro 
65                  70                  75                  80  
Leu Phe His Pro Asn Val Tyr Pro Ser Gly Thr Val Cys Leu Ser Ile 
                85                  90                  95      
Leu Glu Glu Asp Lys Asp Trp Arg Pro Ala Ile Thr Ile Lys Gln Ile 
            100                 105                 110         
Leu Leu Gly Ile Gln Glu Leu Leu Asn Glu Pro Asn Ile Gln Asp Pro 
        115                 120                 125             
Ala Gln Ala Glu Ala Tyr Thr Ile Tyr Cys Gln Asn Arg Val Glu Tyr 
    130                 135                 140                 
Glu Lys Arg Val Arg Ala Gln Ala Lys Lys Phe Ala Pro Ser 
145                 150                 155             

<210> 2
<211> 158
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Ubc9_02

<400> 2
Met Ser Gly Ile Ala Leu Ser Arg Leu Ala Gln Glu Arg Arg Ala Trp 
1               5                   10                  15      
Arg Lys Asp His Pro Phe Gly Phe Val Ala Val Pro Thr Lys Asn Pro 
            20                  25                  30          
Asp Gly Thr Met Asn Leu Met Asn Trp Glu Cys Ala Ile Pro Gly Lys 
        35                  40                  45              
Lys Gly Thr Pro Trp Glu Gly Gly Leu Phe Lys Leu Arg Met Leu Phe 
    50                  55                  60                  
Lys Asp Asp Tyr Pro Ser Ser Pro Pro Lys Cys Lys Phe Glu Pro Pro 
65                  70                  75                  80  
Leu Phe His Pro Asn Val Tyr Pro Ser Gly Thr Val Cys Leu Ser Ile 
                85                  90                  95      
Leu Glu Glu Asp Lys Asp Trp Arg Pro Ala Ile Thr Ile Lys Gln Ile 
            100                 105                 110         
Leu Leu Gly Ile Gln Glu Leu Leu Asn Glu Pro Asn Ile Gln Asp Pro 
        115                 120                 125             
Ala Gln Ala Glu Ala Tyr Thr Ile Tyr Cys Gln Asn Arg Val Glu Tyr 
    130                 135                 140                 
Glu Lys Arg Val Arg Ala Gln Ala Lys Lys Phe Ala Pro Ser 
145                 150                 155             

<210> 3
<211> 158
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Ubc9_03

<400> 3
Met Ser Gly Ile Ala Leu Ser Arg Leu Ala Gln Glu Arg Lys Ala Trp 
1               5                   10                  15      
Arg Lys Asp His Pro Phe Gly Phe Val Ala Val Pro Thr Lys Asn Pro 
            20                  25                  30          
Asp Gly Thr Met Asn Leu Met Asn Trp Glu Cys Ala Ile Pro Gly Lys 
        35                  40                  45              
Lys Gly Thr Pro Trp Glu Gly Gly Leu Phe Lys Leu Arg Met Leu Phe 
    50                  55                  60                  
Lys Asp Asp Tyr Pro Ser Ser Pro Pro Lys Cys Lys Phe Glu Pro Pro 
65                  70                  75                  80  
Leu Phe His Pro Asn Val Tyr Pro Ser Gly Thr Val Cys Leu Ser Ile 
                85                  90                  95      
Leu Glu Glu Asp Lys Asp Trp Arg Pro Ala Ile Thr Ile Lys Gln Ile 
            100                 105                 110         
Leu Leu Gly Ile Gln Glu Leu Leu Asn Glu Pro Asn Ile Gln Asp Pro 
        115                 120                 125             
Ala Gln Ala Glu Ala Tyr Thr Ile Tyr Ala Gln Asn Arg Val Glu Tyr 
    130                 135                 140                 
Glu Lys Arg Val Arg Ala Gln Ala Lys Lys Phe Ala Pro Ser 
145                 150                 155             

<210> 4
<211> 158
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Ubc9_04

<400> 4
Met Ser Gly Ile Ala Leu Ser Arg Leu Ala Gln Glu Arg Arg Ala Trp 
1               5                   10                  15      
Arg Lys Asp His Pro Phe Gly Phe Val Ala Val Pro Thr Lys Asn Pro 
            20                  25                  30          
Asp Gly Thr Met Asn Leu Met Asn Trp Glu Cys Ala Ile Pro Gly Lys 
        35                  40                  45              
Lys Gly Thr Pro Trp Glu Gly Gly Leu Phe Lys Leu Arg Met Leu Phe 
    50                  55                  60                  
Lys Asp Asp Tyr Pro Ser Ser Pro Pro Lys Cys Lys Phe Glu Pro Pro 
65                  70                  75                  80  
Leu Phe His Pro Asn Val Tyr Pro Ser Gly Thr Val Cys Leu Ser Ile 
                85                  90                  95      
Leu Glu Glu Asp Lys Asp Trp Arg Pro Ala Ile Thr Ile Lys Gln Ile 
            100                 105                 110         
Leu Leu Gly Ile Gln Glu Leu Leu Asn Glu Pro Asn Ile Gln Asp Pro 
        115                 120                 125             
Ala Gln Ala Glu Ala Tyr Thr Ile Tyr Ala Gln Asn Arg Val Glu Tyr 
    130                 135                 140                 
Glu Lys Arg Val Arg Ala Gln Ala Lys Lys Phe Ala Pro Ser 
145                 150                 155             

<210> 5
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Donor tag 1

<400> 5
Gln Glu Gln Thr Gly Gly 
1               5       

<210> 6
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Donor tag 2

<400> 6
Gln Gln Gln Thr Gly Gly 
1               5       

<210> 7
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Donor tag 3

<400> 7
Gln Gln Pro Thr Gly Gly 
1               5       

<210> 8
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Donor tag 4

<400> 8
Arg Leu Arg Gly Gly 
1               5   

<210> 9
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Donor tag 5

<400> 9
Leu Arg Leu Arg Gly Gly 
1               5       

<210> 10
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_01

<220> 
<221> VARIANT
<222> 1
<223> X can be any hydrophobic amino acid

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 10
Xaa Lys Xaa Asp 
1               

<210> 11
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_02

<220> 
<221> VARIANT
<222> 1
<223> X can be any hydrophobic amino acid

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 11
Xaa Lys Xaa Glu 
1               

<210> 12
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_03

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 12
Gly Lys Xaa Asp 
1               

<210> 13
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2 _04

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 13
Ala Lys Xaa Asp 
1               

<210> 14
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2 _05

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 14
Val Lys Xaa Asp 
1               

<210> 15
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_06

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 15
Leu Lys Xaa Asp 
1               

<210> 16
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_07

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 16
Ile Lys Xaa Asp 
1               

<210> 17
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_08

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 17
Pro Lys Xaa Asp 
1               

<210> 18
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_09

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 18
Phe Lys Xaa Asp 
1               

<210> 19
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_10

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 19
Met Lys Xaa Asp 
1               

<210> 20
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_11

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 20
Trp Lys Xaa Asp 
1               

<210> 21
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_12

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 21
Gly Lys Xaa Glu 
1               

<210> 22
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_13

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 22
Ala Lys Xaa Glu 
1               

<210> 23
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_14

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 23
Val Lys Xaa Glu 
1               

<210> 24
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_15

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 24
Leu Lys Xaa Glu 
1               

<210> 25
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_16

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 25
Ile Lys Xaa Glu 
1               

<210> 26
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_17

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 26
Pro Lys Xaa Glu 
1               

<210> 27
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_18

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 27
Phe Lys Xaa Glu 
1               

<210> 28
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_19

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 28
Met Lys Xaa Glu 
1               

<210> 29
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_20

<220> 
<221> VARIANT
<222> 3
<223> X can be any natural amino acid

<400> 29
Trp Lys Xaa Glu 
1               

<210> 30
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_21

<400> 30
Pro Arg Ala Val Ile Lys Gln Glu Ser Glu Glu 
1               5                   10      

<210> 31
<211> 14
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_22

<400> 31
Gly Ser Gly Pro Arg Ala Val Ile Lys Gln Glu Ser Glu Glu 
1               5                   10                  

<210> 32
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<223> His6-tag

<400> 32
Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro Arg 
1               5                   10                  15      
Gly Ser His Met 
            20  

<210> 33
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<221> BINDING
<222> 1
<223> sulforhodamine B

<220> 
<223> sulforhodamine-B-LRLRGG-SR1 thioester

<220> 
<221> THIOLEST
<222> 6
<223> S-Ac-Cys-NHMe-thioester

<400> 33
Leu Arg Leu Arg Gly Gly 
1               5       

<210> 34
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> GlyGlyGlyLys(coumarin)

<220> 
<221> BINDING
<222> 4
<223> 7-(Diethylamino)-3-carboxycoumarin

<400> 34
Gly Gly Gly Lys 
1               

<210> 35
<211> 19
<212> PRT
<213> Artificial Sequence


<220> 
<223> Sortase-reactive tag

<400> 35
Gly Gly Ser Gly Arg Ser Leu Pro Glu Thr Gly Gly His His His His 
1               5                   10                  15      
His His Val 
            

<210> 36
<211> 14
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_23

<400> 36
Gly Ser Gly Pro Arg Lys Val Ile Lys Met Glu Ser Glu Glu 
1               5                   10                  

<210> 37
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_24

<400> 37
Gly Ser Gly Pro Arg Lys Val Ile Lys Met Glu Ser Glu Glu Gly Ser 
1               5                   10                  15      



<210> 38
<211> 19
<212> PRT
<213> Artificial Sequence


<220> 
<223> Acceptor tag T2_25

<400> 38
Gly Gly Ser Gly Arg Gly Ser Gly Pro Arg Ala Val Ile Lys Gln Glu 
1               5                   10                  15      
Ser Glu Glu 
            

<210> 39
<211> 158
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Ubc9_05

<400> 39
Met Ser Gly Ile Ala Leu Ser Arg Leu Ala Gln Glu Ala Lys Ala Trp 
1               5                   10                  15      
Arg Lys Asp His Pro Phe Gly Phe Val Ala Val Pro Thr Lys Asn Pro 
            20                  25                  30          
Asp Gly Thr Met Asn Leu Met Asn Trp Glu Cys Ala Ile Pro Gly Lys 
        35                  40                  45              
Lys Gly Thr Pro Trp Glu Gly Gly Leu Phe Lys Leu Arg Met Leu Phe 
    50                  55                  60                  
Lys Asp Asp Tyr Pro Ser Ser Pro Pro Lys Cys Lys Phe Glu Pro Pro 
65                  70                  75                  80  
Leu Phe His Pro Asn Val Tyr Pro Ser Gly Thr Val Cys Leu Ser Ile 
                85                  90                  95      
Leu Glu Glu Asp Lys Asp Trp Arg Pro Ala Ile Thr Ile Lys Gln Ile 
            100                 105                 110         
Leu Leu Gly Ile Gln Glu Leu Leu Asn Glu Pro Asn Ile Gln Asp Pro 
        115                 120                 125             
Ala Gln Ala Glu Ala Tyr Thr Ile Tyr Cys Gln Asn Arg Val Glu Tyr 
    130                 135                 140                 
Glu Lys Arg Val Arg Ala Gln Ala Lys Lys Phe Ala Pro Ser 
145                 150                 155             

<210> 40
<211> 158
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Ubc9_06

<400> 40
Met Ser Gly Ile Ala Leu Ser Arg Leu Ala Gln Glu Arg Lys Ala Trp 
1               5                   10                  15      
Arg Lys Asp His Pro Phe Gly Phe Val Ala Val Pro Thr Lys Asn Pro 
            20                  25                  30          
Asp Gly Thr Met Asn Leu Met Asn Trp Glu Cys Ala Ile Pro Gly Lys 
        35                  40                  45              
Lys Gly Thr Pro Trp Glu Gly Gly Leu Phe Lys Leu Arg Met Leu Phe 
    50                  55                  60                  
Lys Asp Asp Tyr Pro Ser Ser Pro Pro Lys Cys Lys Phe Glu Pro Pro 
65                  70                  75                  80  
Leu Phe His Pro Asn Val Tyr Pro Ser Gly Thr Val Cys Leu Ser Ile 
                85                  90                  95      
Leu Glu Glu Asp Lys Ala Trp Arg Pro Ala Ile Thr Ile Lys Gln Ile 
            100                 105                 110         
Leu Leu Gly Ile Gln Glu Leu Leu Asn Glu Pro Asn Ile Gln Asp Pro 
        115                 120                 125             
Ala Gln Ala Glu Ala Tyr Thr Ile Tyr Cys Gln Asn Arg Val Glu Tyr 
    130                 135                 140                 
Glu Lys Arg Val Arg Ala Gln Ala Lys Lys Phe Ala Pro Ser 
145                 150                 155             

<210> 41
<211> 158
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Ubc9_07

<400> 41
Met Ser Gly Ile Ala Leu Ser Arg Leu Ala Gln Glu Arg Lys Ala Trp 
1               5                   10                  15      
Arg Lys Asp His Pro Phe Gly Phe Val Ala Val Pro Thr Lys Asn Pro 
            20                  25                  30          
Asp Gly Thr Met Asn Leu Met Asn Trp Glu Cys Ala Ile Pro Gly Lys 
        35                  40                  45              
Lys Gly Thr Pro Trp Glu Gly Gly Leu Phe Lys Leu Arg Met Leu Phe 
    50                  55                  60                  
Lys Asp Asp Tyr Pro Ser Ser Pro Pro Lys Cys Lys Phe Glu Pro Pro 
65                  70                  75                  80  
Leu Phe His Pro Asn Val Tyr Pro Ser Gly Thr Val Cys Leu Ser Ile 
                85                  90                  95      
Leu Glu Glu Asp Lys Arg Trp Arg Pro Ala Ile Thr Ile Lys Gln Ile 
            100                 105                 110         
Leu Leu Gly Ile Gln Glu Leu Leu Asn Glu Pro Asn Ile Gln Asp Pro 
        115                 120                 125             
Ala Gln Ala Glu Ala Tyr Thr Ile Tyr Cys Gln Asn Arg Val Glu Tyr 
    130                 135                 140                 
Glu Lys Arg Val Arg Ala Gln Ala Lys Lys Phe Ala Pro Ser 
145                 150                 155             

<210> 42
<211> 158
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Ubc9_08

<400> 42
Met Ser Gly Ile Ala Leu Ser Arg Leu Ala Gln Glu Arg Lys Ala Trp 
1               5                   10                  15      
Arg Lys Asp His Pro Phe Gly Phe Val Ala Val Pro Thr Lys Asn Pro 
            20                  25                  30          
Asp Gly Thr Met Asn Leu Met Asn Trp Glu Cys Ala Ile Pro Gly Lys 
        35                  40                  45              
Lys Gly Thr Pro Trp Glu Gly Gly Leu Phe Lys Leu Arg Met Leu Phe 
    50                  55                  60                  
Lys Asp Asp Tyr Pro Ser Ser Pro Pro Lys Cys Lys Phe Glu Pro Pro 
65                  70                  75                  80  
Leu Phe His Pro Asn Val Tyr Pro Ser Gly Thr Val Cys Leu Ser Ile 
                85                  90                  95      
Leu Glu Glu Asp Lys Asp Trp Arg Pro Ala Ile Thr Ile Lys Gln Ile 
            100                 105                 110         
Leu Leu Gly Ile Gln Ala Leu Leu Asn Glu Pro Asn Ile Gln Asp Pro 
        115                 120                 125             
Ala Gln Ala Glu Ala Tyr Thr Ile Tyr Cys Gln Asn Arg Val Glu Tyr 
    130                 135                 140                 
Glu Lys Arg Val Arg Ala Gln Ala Lys Lys Phe Ala Pro Ser 
145                 150                 155             

<210> 43
<211> 158
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Ubc9_09

<400> 43
Met Ser Gly Ile Ala Leu Ser Arg Leu Ala Gln Glu Arg Lys Ala Trp 
1               5                   10                  15      
Arg Lys Asp His Pro Phe Gly Phe Val Ala Val Pro Thr Lys Asn Pro 
            20                  25                  30          
Asp Gly Thr Met Asn Leu Met Asn Trp Glu Cys Ala Ile Pro Gly Lys 
        35                  40                  45              
Lys Gly Thr Pro Trp Glu Gly Gly Leu Phe Lys Leu Arg Met Leu Phe 
    50                  55                  60                  
Lys Asp Asp Tyr Pro Ser Ser Pro Pro Lys Cys Lys Phe Glu Pro Pro 
65                  70                  75                  80  
Leu Phe His Pro Asn Val Tyr Pro Ser Gly Thr Val Cys Leu Ser Ile 
                85                  90                  95      
Leu Glu Glu Asp Lys Asp Trp Arg Pro Ala Ile Thr Ile Lys Gln Ile 
            100                 105                 110         
Leu Leu Gly Ile Gln Arg Leu Leu Asn Glu Pro Asn Ile Gln Asp Pro 
        115                 120                 125             
Ala Gln Ala Glu Ala Tyr Thr Ile Tyr Cys Gln Asn Arg Val Glu Tyr 
    130                 135                 140                 
Glu Lys Arg Val Arg Ala Gln Ala Lys Lys Phe Ala Pro Ser 
145                 150                 155             

<210> 44
<211> 158
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Ubc9_10

<400> 44
Met Ser Gly Ile Ala Leu Ser Arg Leu Ala Gln Glu Arg Lys Ala Trp 
1               5                   10                  15      
Arg Lys Asp His Pro Phe Gly Phe Val Ala Val Pro Thr Lys Asn Pro 
            20                  25                  30          
Asp Gly Thr Met Asn Leu Met Asn Trp Glu Cys Ala Ile Pro Gly Lys 
        35                  40                  45              
Lys Gly Thr Pro Trp Glu Gly Gly Leu Phe Lys Leu Arg Met Leu Phe 
    50                  55                  60                  
Lys Asp Asp Tyr Pro Ser Ser Pro Pro Lys Cys Lys Phe Glu Pro Pro 
65                  70                  75                  80  
Leu Phe His Pro Asn Val Tyr Pro Ser Gly Thr Val Cys Leu Ser Ile 
                85                  90                  95      
Leu Glu Glu Asp Lys Asp Trp Arg Pro Ala Ile Thr Ile Lys Gln Ile 
            100                 105                 110         
Leu Leu Gly Ile Gln Glu Leu Leu Asn Ala Pro Asn Ile Gln Asp Pro 
        115                 120                 125             
Ala Gln Ala Glu Ala Tyr Thr Ile Tyr Cys Gln Asn Arg Val Glu Tyr 
    130                 135                 140                 
Glu Lys Arg Val Arg Ala Gln Ala Lys Lys Phe Ala Pro Ser 
145                 150                 155             

<210> 45
<211> 158
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified_Ubc9_11

<400> 45
Met Ser Gly Ile Ala Leu Ser Arg Leu Ala Gln Glu Arg Lys Ala Trp 
1               5                   10                  15      
Arg Lys Asp His Pro Phe Gly Phe Val Ala Val Pro Thr Lys Asn Pro 
            20                  25                  30          
Asp Gly Thr Met Asn Leu Met Asn Trp Glu Cys Ala Ile Pro Gly Lys 
        35                  40                  45              
Lys Gly Thr Pro Trp Glu Gly Gly Leu Phe Lys Leu Arg Met Leu Phe 
    50                  55                  60                  
Lys Asp Asp Tyr Pro Ser Ser Pro Pro Lys Cys Lys Phe Glu Pro Pro 
65                  70                  75                  80  
Leu Phe His Pro Asn Val Tyr Pro Ser Gly Thr Val Cys Leu Ser Ile 
                85                  90                  95      
Leu Glu Glu Asp Lys Asp Trp Arg Pro Ala Ile Thr Ile Lys Gln Ile 
            100                 105                 110         
Leu Leu Gly Ile Gln Glu Leu Leu Asn Arg Pro Asn Ile Gln Asp Pro 
        115                 120                 125             
Ala Gln Ala Glu Ala Tyr Thr Ile Tyr Cys Gln Asn Arg Val Glu Tyr 
    130                 135                 140                 
Glu Lys Arg Val Arg Ala Gln Ala Lys Lys Phe Ala Pro Ser 
145                 150                 155             

