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<110>  ���ŷ���ũҵ�Ƽ��о�Ժ
 
<120>  Ԥ������������Ⱦ�����鵰���ʼ����������Ｐϸ��

<130>  WPI19TW0325

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  365
<212>  PRT
<213>  swine fever virus

<400>  1

Val Lys Val Leu Arg Gly Gln Ile Val Gln Gly Val Ile Trp Leu Leu 
1               5                   10                  15      


Leu Val Thr Gly Ala Gln Gly Arg Leu Ala Cys Lys Glu Asp Tyr Arg 
            20                  25                  30          


Tyr Ala Ile Ser Ser Thr Asp Glu Ile Gly Leu Leu Gly Ala Gly Gly 
        35                  40                  45              


Leu Thr Thr Thr Trp Lys Glu Tyr Thr His Asp Leu Gln Leu Asn Asp 
    50                  55                  60                  


Gly Thr Val Lys Ala Thr Cys Val Ala Gly Ser Phe Lys Val Thr Ala 
65                  70                  75                  80  


Leu Asn Val Val Ser Arg Arg Tyr Leu Ala Ser Leu His Lys Lys Ala 
                85                  90                  95      


Leu Pro Thr Ser Val Thr Phe Glu Leu Leu Phe Asp Gly Thr Asn Pro 
            100                 105                 110         


Ser Thr Glu Glu Met Gly Asp Asp Phe Gly Phe Gly Leu Cys Pro Phe 
        115                 120                 125             


Asp Thr Arg Pro Val Val Lys Gly Lys Tyr Asn Ala Thr Leu Val Asn 
    130                 135                 140                 


Gly Ser Ala Phe Tyr Leu Val Cys Pro Ile Gly Trp Thr Gly Val Ile 
145                 150                 155                 160 


Glu Cys Thr Ala Val Ser Pro Thr Thr Leu Arg Thr Glu Val Val Lys 
                165                 170                 175     


Thr Phe Arg Arg Asp Lys Pro Phe Pro His Arg Met Asn Cys Val Thr 
            180                 185                 190         


Thr Thr Val Glu Asn Glu Asp Leu Phe Tyr Cys Lys Leu Gly Gly Asn 
        195                 200                 205             


Trp Thr Cys Val Lys Gly Glu Pro Val Val Tyr Thr Gly Gly Leu Val 
    210                 215                 220                 


Lys Gln Cys Arg Trp Cys Gly Phe Asp Phe Asn Glu Pro Asp Gly Leu 
225                 230                 235                 240 


Pro His Tyr Pro Ile Gly Lys Cys Ile Leu Ala Asn Glu Thr Ser Tyr 
                245                 250                 255     


Arg Val Val Asp Ser Thr Asp Cys Asn Arg Asp Gly Val Val Ile Ser 
            260                 265                 270         


Thr Glu Gly Ser His Glu Cys Leu Ile Gly Asn Thr Thr Val Lys Val 
        275                 280                 285             


His Ala Ser Asp Glu Arg Leu Gly Pro Met Pro Cys Arg Pro Lys Glu 
    290                 295                 300                 


Ile Val Ser Ser Ala Gly Pro Ala Met Lys Thr Ser Cys Thr Phe Asn 
305                 310                 315                 320 


Tyr Ala Lys Thr Leu Lys Asn Arg Tyr Tyr Glu Pro Arg Asp Ser Tyr 
                325                 330                 335     


Phe Gln Gln Tyr Met Leu Lys Gly Glu Tyr Gln Tyr Trp Phe Asp Leu 
            340                 345                 350         


Asp Ala Thr Asp Arg His Ser Asp Tyr Phe Ala Glu Phe 
        355                 360                 365 


<210>  2
<211>  165
<212>  PRT
<213>  Helicobacter pylori

<400>  2

Asp Ile Ile Lys Leu Leu Asn Glu Gln Val Asn Lys Glu Met Gln Ser 
1               5                   10                  15      


Ser Asn Leu Tyr Met Ser Met Ser Ser Trp Cys Tyr Thr His Ser Leu 
            20                  25                  30          


Asp Gly Ala Gly Leu Phe Leu Phe Asp His Ala Ala Glu Glu Tyr Glu 
        35                  40                  45              


His Ala Lys Lys Leu Ile Ile Phe Leu Asn Glu Asn Asn Val Pro Val 
    50                  55                  60                  


Gln Leu Thr Ser Ile Ser Ala Pro Glu His Lys Phe Glu Gly Leu Thr 
65                  70                  75                  80  


Gln Ile Phe Gln Lys Ala Tyr Glu His Glu Gln His Ile Ser Glu Ser 
                85                  90                  95      


Ile Asn Asn Ile Val Asp His Ala Ile Lys Ser Lys Asp His Ala Thr 
            100                 105                 110         


Phe Asn Phe Leu Gln Trp Tyr Val Ala Glu Gln His Glu Glu Glu Val 
        115                 120                 125             


Leu Phe Lys Asp Ile Leu Asp Lys Ile Glu Leu Ile Gly Asn Glu Asn 
    130                 135                 140                 


His Gly Leu Tyr Leu Ala Asp Gln Tyr Val Lys Gly Ile Ala Lys Ser 
145                 150                 155                 160 


Arg Lys Ser Gly Ser 
                165 


<210>  3
<211>  1095
<212>  DNA
<213>  swine fever virus

<400>  3
gtgaaagtgc tgcgtggtca gattgtccag ggggtcattt ggctgctgct ggtgactggc       60

gctcagggaa gactggcatg caaggaggac taccgctatg caatctccag cacagatgaa      120

attggactgc tgggtgcagg aggactgacc acaacttgga aggagtacac acacgacctg      180

cagctgaatg atggaaccgt caaggcaaca tgcgtggccg ggtctttcaa agtgacagct      240

ctgaacgtgg tcagtaggcg gtatctggcc tcactgcata agaaagctct gcctacttct      300

gtgaccttcg agctgctgtt tgacggcacc aatccaagta cagaggaaat gggcgacgat      360

ttcggctttg gactgtgccc ctttgatacc aggcctgtgg tcaagggtaa atacaacgcc      420

acactggtga atggctccgc cttctatctg gtgtgcccca tcggctggac cggagtgatt      480

gagtgtacag cagtgtctcc tactaccctg agaactgaag tggtcaagac cttcagacgc      540

gacaaaccct ttcctcaccg catgaactgc gtcacaacta ccgtggagaa cgaagacctg      600

ttttactgca agctgggggg taattggaca tgcgtgaaag gcgagccagt ggtctatact      660

ggcggactgg tcaagcagtg cagatggtgt ggattcgact ttaatgagcc cgatggtctg      720

cctcattacc caatcggaaa atgtattctg gccaacgaaa cttcctatcg agtggtggac      780

agtaccgatt gcaatcgtga cggggtggtc atctcaaccg agggttccca cgaatgtctg      840

attggcaaca caactgtcaa ggtgcatgct tccgatgaga ggctgggacc aatgccctgc      900

cggccaaagg aaatcgtgtc ctccgccggc cccgctatga aaacatcatg tactttcaac      960

tacgctaaga cactgaaaaa tcgatactat gagccccgtg actcctactt ccagcagtat     1020

atgctgaagg gcgaatacca gtattggttt gacctggatg caaccgaccg acactccgat     1080

tacttcgccg agttt                                                      1095


<210>  4
<211>  495
<212>  DNA
<213>  Helicobacter pylori

<400>  4
gatattatca agctgctgaa cgaacaggtg aacaaggaga tgcagagctc caacctgtac       60

atgagtatgt ctagttggtg ctatactcac tccctggacg gcgctggact gttcctgttt      120

gatcacgccg ctgaggaata cgaacatgca aagaaactga tcattttcct gaatgagaac      180

aatgtgcccg tccagctgac ctcaatcagc gcccctgaac ataagttcga gggactgaca      240

cagatctttc agaaggccta cgaacacgag cagcatattt ccgagtctat caacaatatt      300

gtcgaccacg caatcaagag caaagatcat gccaccttca acttcctcca gtggtacgtg      360

gccgagcagc acgaggaaga ggtcctgttt aaggacattc tggataaaat cgaactgatt      420

gggaacgaga atcatggcct gtacctggca gatcagtatg tgaaaggcat cgcaaagtcc      480

cgaaaaagcg gctcc                                                       495


<210>  5
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  5

Cys Pro Gly Gly Ser 
1               5   


<210>  6
<211>  535
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant protein

<400>  6

Val Lys Val Leu Arg Gly Gln Ile Val Gln Gly Val Ile Trp Leu Leu 
1               5                   10                  15      


Leu Val Thr Gly Ala Gln Gly Arg Leu Ala Cys Lys Glu Asp Tyr Arg 
            20                  25                  30          


Tyr Ala Ile Ser Ser Thr Asp Glu Ile Gly Leu Leu Gly Ala Gly Gly 
        35                  40                  45              


Leu Thr Thr Thr Trp Lys Glu Tyr Thr His Asp Leu Gln Leu Asn Asp 
    50                  55                  60                  


Gly Thr Val Lys Ala Thr Cys Val Ala Gly Ser Phe Lys Val Thr Ala 
65                  70                  75                  80  


Leu Asn Val Val Ser Arg Arg Tyr Leu Ala Ser Leu His Lys Lys Ala 
                85                  90                  95      


Leu Pro Thr Ser Val Thr Phe Glu Leu Leu Phe Asp Gly Thr Asn Pro 
            100                 105                 110         


Ser Thr Glu Glu Met Gly Asp Asp Phe Gly Phe Gly Leu Cys Pro Phe 
        115                 120                 125             


Asp Thr Arg Pro Val Val Lys Gly Lys Tyr Asn Ala Thr Leu Val Asn 
    130                 135                 140                 


Gly Ser Ala Phe Tyr Leu Val Cys Pro Ile Gly Trp Thr Gly Val Ile 
145                 150                 155                 160 


Glu Cys Thr Ala Val Ser Pro Thr Thr Leu Arg Thr Glu Val Val Lys 
                165                 170                 175     


Thr Phe Arg Arg Asp Lys Pro Phe Pro His Arg Met Asn Cys Val Thr 
            180                 185                 190         


Thr Thr Val Glu Asn Glu Asp Leu Phe Tyr Cys Lys Leu Gly Gly Asn 
        195                 200                 205             


Trp Thr Cys Val Lys Gly Glu Pro Val Val Tyr Thr Gly Gly Leu Val 
    210                 215                 220                 


Lys Gln Cys Arg Trp Cys Gly Phe Asp Phe Asn Glu Pro Asp Gly Leu 
225                 230                 235                 240 


Pro His Tyr Pro Ile Gly Lys Cys Ile Leu Ala Asn Glu Thr Ser Tyr 
                245                 250                 255     


Arg Val Val Asp Ser Thr Asp Cys Asn Arg Asp Gly Val Val Ile Ser 
            260                 265                 270         


Thr Glu Gly Ser His Glu Cys Leu Ile Gly Asn Thr Thr Val Lys Val 
        275                 280                 285             


His Ala Ser Asp Glu Arg Leu Gly Pro Met Pro Cys Arg Pro Lys Glu 
    290                 295                 300                 


Ile Val Ser Ser Ala Gly Pro Ala Met Lys Thr Ser Cys Thr Phe Asn 
305                 310                 315                 320 


Tyr Ala Lys Thr Leu Lys Asn Arg Tyr Tyr Glu Pro Arg Asp Ser Tyr 
                325                 330                 335     


Phe Gln Gln Tyr Met Leu Lys Gly Glu Tyr Gln Tyr Trp Phe Asp Leu 
            340                 345                 350         


Asp Ala Thr Asp Arg His Ser Asp Tyr Phe Ala Glu Phe Cys Pro Gly 
        355                 360                 365             


Gly Ser Asp Ile Ile Lys Leu Leu Asn Glu Gln Val Asn Lys Glu Met 
    370                 375                 380                 


Gln Ser Ser Asn Leu Tyr Met Ser Met Ser Ser Trp Cys Tyr Thr His 
385                 390                 395                 400 


Ser Leu Asp Gly Ala Gly Leu Phe Leu Phe Asp His Ala Ala Glu Glu 
                405                 410                 415     


Tyr Glu His Ala Lys Lys Leu Ile Ile Phe Leu Asn Glu Asn Asn Val 
            420                 425                 430         


Pro Val Gln Leu Thr Ser Ile Ser Ala Pro Glu His Lys Phe Glu Gly 
        435                 440                 445             


Leu Thr Gln Ile Phe Gln Lys Ala Tyr Glu His Glu Gln His Ile Ser 
    450                 455                 460                 


Glu Ser Ile Asn Asn Ile Val Asp His Ala Ile Lys Ser Lys Asp His 
465                 470                 475                 480 


Ala Thr Phe Asn Phe Leu Gln Trp Tyr Val Ala Glu Gln His Glu Glu 
                485                 490                 495     


Glu Val Leu Phe Lys Asp Ile Leu Asp Lys Ile Glu Leu Ile Gly Asn 
            500                 505                 510         


Glu Asn His Gly Leu Tyr Leu Ala Asp Gln Tyr Val Lys Gly Ile Ala 
        515                 520                 525             


Lys Ser Arg Lys Ser Gly Ser 
    530                 535 


<210>  7
<211>  1605
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence encoding recombinant protein

<400>  7
gtgaaagtgc tgcgtggtca gattgtccag ggggtcattt ggctgctgct ggtgactggc       60

gctcagggaa gactggcatg caaggaggac taccgctatg caatctccag cacagatgaa      120

attggactgc tgggtgcagg aggactgacc acaacttgga aggagtacac acacgacctg      180

cagctgaatg atggaaccgt caaggcaaca tgcgtggccg ggtctttcaa agtgacagct      240

ctgaacgtgg tcagtaggcg gtatctggcc tcactgcata agaaagctct gcctacttct      300

gtgaccttcg agctgctgtt tgacggcacc aatccaagta cagaggaaat gggcgacgat      360

ttcggctttg gactgtgccc ctttgatacc aggcctgtgg tcaagggtaa atacaacgcc      420

acactggtga atggctccgc cttctatctg gtgtgcccca tcggctggac cggagtgatt      480

gagtgtacag cagtgtctcc tactaccctg agaactgaag tggtcaagac cttcagacgc      540

gacaaaccct ttcctcaccg catgaactgc gtcacaacta ccgtggagaa cgaagacctg      600

ttttactgca agctgggggg taattggaca tgcgtgaaag gcgagccagt ggtctatact      660

ggcggactgg tcaagcagtg cagatggtgt ggattcgact ttaatgagcc cgatggtctg      720

cctcattacc caatcggaaa atgtattctg gccaacgaaa cttcctatcg agtggtggac      780

agtaccgatt gcaatcgtga cggggtggtc atctcaaccg agggttccca cgaatgtctg      840

attggcaaca caactgtcaa ggtgcatgct tccgatgaga ggctgggacc aatgccctgc      900

cggccaaagg aaatcgtgtc ctccgccggc cccgctatga aaacatcatg tactttcaac      960

tacgctaaga cactgaaaaa tcgatactat gagccccgtg actcctactt ccagcagtat     1020

atgctgaagg gcgaatacca gtattggttt gacctggatg caaccgaccg acactccgat     1080

tacttcgccg agttttgtcc aggggggtct gatattatca agctgctgaa cgaacaggtg     1140

aacaaggaga tgcagagctc caacctgtac atgagtatgt ctagttggtg ctatactcac     1200

tccctggacg gcgctggact gttcctgttt gatcacgccg ctgaggaata cgaacatgca     1260

aagaaactga tcattttcct gaatgagaac aatgtgcccg tccagctgac ctcaatcagc     1320

gcccctgaac ataagttcga gggactgaca cagatctttc agaaggccta cgaacacgag     1380

cagcatattt ccgagtctat caacaatatt gtcgaccacg caatcaagag caaagatcat     1440

gccaccttca acttcctcca gtggtacgtg gccgagcagc acgaggaaga ggtcctgttt     1500

aaggacattc tggataaaat cgaactgatt gggaacgaga atcatggcct gtacctggca     1560

gatcagtatg tgaaaggcat cgcaaagtcc cgaaaaagcg gctcc                     1605


<210>  8
<211>  2348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette

<400>  8
agatctcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata       60

gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact      120

tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat      180

gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggacta      240

tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc      300

tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg      360

ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg      420

gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct      480

ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa      540

atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt      600

ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat      660

gctagcatgg agacagacac actcctgcta tgggtactgc tgctctgggt tccaggttcc      720

actggtgacg gcgcgccgtg aaagtgctgc gtggtcagat tgtccagggg gtcatttggc      780

tgctgctggt gactggcgct cagggaagac tggcatgcaa ggaggactac cgctatgcaa      840

tctccagcac agatgaaatt ggactgctgg gtgcaggagg actgaccaca acttggaagg      900

agtacacaca cgacctgcag ctgaatgatg gaaccgtcaa ggcaacatgc gtggccgggt      960

ctttcaaagt gacagctctg aacgtggtca gtaggcggta tctggcctca ctgcataaga     1020

aagctctgcc tacttctgtg accttcgagc tgctgtttga cggcaccaat ccaagtacag     1080

aggaaatggg cgacgatttc ggctttggac tgtgcccctt tgataccagg cctgtggtca     1140

agggtaaata caacgccaca ctggtgaatg gctccgcctt ctatctggtg tgccccatcg     1200

gctggaccgg agtgattgag tgtacagcag tgtctcctac taccctgaga actgaagtgg     1260

tcaagacctt cagacgcgac aaaccctttc ctcaccgcat gaactgcgtc acaactaccg     1320

tggagaacga agacctgttt tactgcaagc tggggggtaa ttggacatgc gtgaaaggcg     1380

agccagtggt ctatactggc ggactggtca agcagtgcag atggtgtgga ttcgacttta     1440

atgagcccga tggtctgcct cattacccaa tcggaaaatg tattctggcc aacgaaactt     1500

cctatcgagt ggtggacagt accgattgca atcgtgacgg ggtggtcatc tcaaccgagg     1560

gttcccacga atgtctgatt ggcaacacaa ctgtcaaggt gcatgcttcc gatgagaggc     1620

tgggaccaat gccctgccgg ccaaaggaaa tcgtgtcctc cgccggcccc gctatgaaaa     1680

catcatgtac tttcaactac gctaagacac tgaaaaatcg atactatgag ccccgtgact     1740

cctacttcca gcagtatatg ctgaagggcg aataccagta ttggtttgac ctggatgcaa     1800

ccgaccgaca ctccgattac ttcgccgagt tttgtccagg ggggtctgat attatcaagc     1860

tgctgaacga acaggtgaac aaggagatgc agagctccaa cctgtacatg agtatgtcta     1920

gttggtgcta tactcactcc ctggacggcg ctggactgtt cctgtttgat cacgccgctg     1980

aggaatacga acatgcaaag aaactgatca ttttcctgaa tgagaacaat gtgcccgtcc     2040

agctgacctc aatcagcgcc cctgaacata agttcgaggg actgacacag atctttcaga     2100

aggcctacga acacgagcag catatttccg agtctatcaa caatattgtc gaccacgcaa     2160

tcaagagcaa agatcatgcc accttcaact tcctccagtg gtacgtggcc gagcagcacg     2220

aggaagaggt cctgtttaag gacattctgg ataaaatcga actgattggg aacgagaatc     2280

atggcctgta cctggcagat cagtatgtga aaggcatcgc aaagtcccga aaaagcggct     2340

ccggatcc                                                              2348

