                         SEQUENCE LISTING

<110>  RENEW BIOPHARMA, INC.
 
<120>  COMPOSITIONS AND METHODS FOR USING GENETICALLY MODIFIED ENZYMES

<130>  REBI-002/00US

<150>  US 62/945,081
<151>  2018-03-19

<160>  604   

<170>  PatentIn version 3.5

<210>  1
<211>  307
<212>  PRT
<213>  Unknown

<220>
<223>  Streptomyces sp. strain CL190

<400>  1

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  2
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A108G, Q295N, L298Q

<400>  2

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Gly Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Asn Arg Gly Gln Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  3
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K119A, Q161S, S177E

<400>  3

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Ala Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Glu Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  4
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49S, M162A, Y283L

<400>  4

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Ser Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Ala Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Leu Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  5
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118Q, Q161W, S214F

<400>  5

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Gln Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Trp Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Phe Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  6
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161A, S214G, V294F

<400>  6

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ala Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Gly Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Phe Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  7
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123W, S177W, G286E

<400>  7

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Trp Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Trp Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Glu Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  8
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118N, N173D, Q295N

<400>  8

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Asn Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asp Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Asn Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  9
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K119A, Q161A, R228Q

<400>  9

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Ala Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ala Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Gln Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  10
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q38G, E112D, F123H

<400>  10

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gly Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Asp 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala His Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  11
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, S177Y, C209G

<400>  11

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Ala Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Tyr Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Gly Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  12
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118N, K119A, V271E

<400>  12

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Asn Ala Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Glu Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  13
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L174V, C230N, L298Q

<400>  13

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Val Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Asn Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Gln Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  14
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K119D, S177E, L219F

<400>  14

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Asp Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Glu Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Phe Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  15
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53E, E112G, R228E

<400>  15

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Glu Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Gly 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Glu Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  16
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53E, F213M, R228Q

<400>  16

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Glu Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Met Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Gln Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  17
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, S177W, L219F

<400>  17

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Gln Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Trp Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Phe Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  18
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123A, Y283L

<400>  18

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Leu Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Ala Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Leu Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  19
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant C25V, Y121W, M162F

<400>  19

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Val Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Trp Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Phe Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  20
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49L, S214R, V271E

<400>  20

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Leu Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Arg Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Glu Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  21
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant D227E, C230N, Q295W

<400>  21

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Glu Arg Leu Asn Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Trp Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  22
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant G205L, Y216A, C230S

<400>  22

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Leu Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Ala Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Ser Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  23
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, V294F, L298W

<400>  23

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Phe Gln Arg Gly Trp Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  24
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L219F, Y283L, L298W

<400>  24

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Phe Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Leu Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Trp Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  25
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A17T, F213M, S214R

<400>  25

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Thr Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Met Arg Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  26
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L219F, V294N, Q295A

<400>  26

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Phe Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Asn Ala Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  27
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53E, A108G, K118N

<400>  27

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Glu Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Gly Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Asn Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  28
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q38G, E112G, M162F

<400>  28

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gly Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Gly 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Phe Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  29
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, D166E, Q295W

<400>  29

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Glu Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Trp Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  30
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L174V, C230S, V294A

<400>  30

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Val Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Ser Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Ala Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  31
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M106E, G286E, L298W

<400>  31

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Glu Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Glu Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Trp Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  32
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant D227E, R228E, L298Q

<400>  32

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Glu Glu Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Gln Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  33
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M162A, C209G, Y288H

<400>  33

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Ala Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Gly Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala His 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  34
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A17T, C25V, E112G

<400>  34

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Thr Ala Gly Leu Leu Gly Val Ala Val Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Gly 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  35
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, Y121W, A232S

<400>  35

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Gln Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Trp Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ser Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  36
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, S177Y, Y288H

<400>  36

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Gln Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Tyr Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala His 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  37
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123H, L274V, L298A

<400>  37

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala His Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Val Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Ala Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  38
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177W, S214F, Y283L

<400>  38

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Trp Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Phe Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Leu Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  39
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53E, G205M, L274V

<400>  39

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Glu Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Met Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Val Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  40
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, K118N, S214F

<400>  40

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Asn Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Phe Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  41
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177E, S214R, R228E

<400>  41

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Glu Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Arg Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Glu Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  42
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118Q, S177Y, D227E

<400>  42

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Gln Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Tyr Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Glu Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  43
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant D166E, S177Y, S214F

<400>  43

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Glu Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Tyr Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Phe Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  44
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161A, M162F, Q295A

<400>  44

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ala Phe Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Ala Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  45
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53E, Q161A, V294N

<400>  45

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Glu Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ala Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Asn Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  46
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A108G, F123W, V294A

<400>  46

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Gly Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Trp Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Ala Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  47
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, G205L, Q295N

<400>  47

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Gln Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Leu Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Asn Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  48
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118Q, F123A, R228E

<400>  48

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Gln Lys Thr Tyr Ala Ala Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Glu Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  49
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49L, E112D, G286E

<400>  49

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Leu Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Asp 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Glu Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  50
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant G205L, R228E, C230N

<400>  50

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Leu Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Glu Leu Asn Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  51
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M162A, D227E, C230S

<400>  51

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Ala Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Glu Arg Leu Ser Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  52
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49L, K119D, G205M

<400>  52

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Leu Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Asp Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Met Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  53
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K119A, S214G, L298A

<400>  53

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Ala Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Gly Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Ala Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  54
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q38G, Y216A, A232S

<400>  54

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gly Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Ala Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ser Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  55
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161A, V271E, Q295W

<400>  55

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ala Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Glu Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Trp Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  56
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49S, Y216A, V294N

<400>  56

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Ser Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Ala Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Asn Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  57
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123A, M162F, S214G

<400>  57

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Ala Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Phe Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Gly Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  58
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant C25V, A232S, V271E

<400>  58

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Val Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ser Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Glu Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  59
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118Q, L174V, R228Q

<400>  59

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Gln Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Val Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Gln Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  60
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49S, S214G, V294A

<400>  60

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Ser Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Gly Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Ala Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  61
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant E112D, K119A, N173D

<400>  61

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Asp 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Ala Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asp Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  62
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123W, Q161W, D227E

<400>  62

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Trp Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Trp Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Glu Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  63
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, N173D, S214R

<400>  63

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asp Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Arg Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  64
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123W, M162F, C209G

<400>  64

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Trp Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Phe Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Gly Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  65
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M106E, M162A, Y216A

<400>  65

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Glu Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Ala Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Ala Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  66
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, Q161S, V294A

<400>  66

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Ala Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Ala Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  67
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant C25V, F213M, Y216A

<400>  67

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Val Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Met Ser Val Ala Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  68
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A108G, K119D, L298A

<400>  68

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Gly Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Asp Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Ala Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  69
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118N, C209G, R228Q

<400>  69

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Asn Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Gly Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Gln Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  70
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, M106E, Q161S

<400>  70

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Glu Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  71
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q38G, D166E, Q295A

<400>  71

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gly Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Glu Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Ala Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  72
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M106E, G205L, C209G

<400>  72

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Glu Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Leu Leu Lys Phe 
        195                 200                 205             


Gly Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  73
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant E112G, G205M, L298W

<400>  73

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Gly 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Met Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Trp Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  74
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49S, K118Q, S177E

<400>  74

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Ser Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Gln Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Glu Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  75
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M106E, Y121W, D166E

<400>  75

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Glu Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Trp Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Glu Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  76
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, L274V, Q295A

<400>  76

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Gln Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Val Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Ala Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  77
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, F123A, Y288H

<400>  77

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Ala Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Ala Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala His 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  78
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant C25V, F123H, Q295N

<400>  78

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Val Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala His Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Asn Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  79
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K119D, Q161W, L298Q

<400>  79

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Asp Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Trp Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Gln Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  80
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, Y121W, C230S

<400>  80

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Ala Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Trp Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Ser Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  81
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M162A, N173D, S214F

<400>  81

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Ala Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asp Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Phe Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  82
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49L, D166E, L274V

<400>  82

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Leu Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Glu Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Val Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  83
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49S, K119D, F213M

<400>  83

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Ser Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Asp Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Met Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  84
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant E112D, L219F, V294F

<400>  84

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Asp 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Phe Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Phe Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  85
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123H, L174V, S177E

<400>  85

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala His Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Val Tyr Phe 
                165                 170                 175     


Glu Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  86
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant E112G, F123H, S214G

<400>  86

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Gly 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala His Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Gly Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  87
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123A, S177W, G205L

<400>  87

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Ala Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Trp Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Leu Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  88
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177W, Y288H, V294N

<400>  88

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Trp Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala His 
        275                 280                 285             


Tyr His Ile Thr Asp Asn Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  89
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A108G, Q161S, G205M

<400>  89

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Gly Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Met Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  90
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y121W, S177Y, G286E

<400>  90

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Trp Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Tyr Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Glu Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  91
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A17T, F123W, L298A

<400>  91

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Thr Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Trp Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Ala Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  92
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant N173D, F213M, V294F

<400>  92

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asp Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Met Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Phe Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  93
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, E112D, G205M

<400>  93

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Asp 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Met Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  94
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q38G, L174V, S214R

<400>  94

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gly Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Val Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Arg Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  95
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A17T, V49A, C230N

<400>  95

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Thr Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Ala Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Asn Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  96
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A17T, Q161W, A232S

<400>  96

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Thr Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Trp Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ser Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  97
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161W, R228Q, Q295W

<400>  97

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Trp Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Gln Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Trp Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  98
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288A

<400>  98

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Ala 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  99
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288C

<400>  99

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Cys 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  100
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288D

<400>  100

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Asp 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  101
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288E

<400>  101

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Glu 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  102
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288F

<400>  102

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Phe 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  103
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288G

<400>  103

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Gly 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  104
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288H

<400>  104

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala His 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  105
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288I

<400>  105

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Ile 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  106
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288K

<400>  106

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Lys 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  107
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288L

<400>  107

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Leu 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  108
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288M

<400>  108

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Met 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  109
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288N

<400>  109

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Asn 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  110
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288P

<400>  110

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Pro 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  111
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288Q

<400>  111

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Gln 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  112
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288R

<400>  112

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Arg 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  113
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288S

<400>  113

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Ser 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  114
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288T

<400>  114

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Thr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  115
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288V

<400>  115

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Val 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  116
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288W

<400>  116

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Trp 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  117
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288Y

<400>  117

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  118
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161A

<400>  118

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ala Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  119
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161C

<400>  119

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Cys Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  120
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161D

<400>  120

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Asp Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  121
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161E

<400>  121

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Glu Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  122
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161F

<400>  122

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Phe Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  123
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161G

<400>  123

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gly Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  124
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161H

<400>  124

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


His Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  125
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161I

<400>  125

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ile Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  126
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161K

<400>  126

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Lys Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  127
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161L

<400>  127

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Leu Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  128
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161M

<400>  128

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Met Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  129
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161N

<400>  129

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Asn Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  130
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161P

<400>  130

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Pro Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  131
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161Q

<400>  131

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  132
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161R

<400>  132

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Arg Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  133
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S

<400>  133

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  134
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161T

<400>  134

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Thr Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  135
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161V

<400>  135

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Val Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  136
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161W

<400>  136

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Trp Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  137
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161Y

<400>  137

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Tyr Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  138
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295A

<400>  138

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Ala Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  139
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295C

<400>  139

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Cys Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  140
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295D

<400>  140

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Asp Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  141
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295E

<400>  141

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Glu Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  142
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295F

<400>  142

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Phe Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  143
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295G

<400>  143

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gly Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  144
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295H

<400>  144

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val His Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  145
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295I

<400>  145

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Ile Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  146
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295K

<400>  146

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Lys Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  147
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295L

<400>  147

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Leu Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  148
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295M

<400>  148

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Met Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  149
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295N

<400>  149

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Asn Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  150
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295P

<400>  150

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Pro Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  151
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295Q

<400>  151

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  152
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295R

<400>  152

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Arg Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  153
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295S

<400>  153

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Ser Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  154
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295T

<400>  154

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Thr Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  155
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295V

<400>  155

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Val Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  156
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295W

<400>  156

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Trp Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  157
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295Y

<400>  157

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Tyr Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  158
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214A

<400>  158

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ala Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  159
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214C

<400>  159

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Cys Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  160
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214D

<400>  160

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Asp Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  161
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214E

<400>  161

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Glu Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  162
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214F

<400>  162

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Phe Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  163
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214G

<400>  163

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Gly Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  164
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214H

<400>  164

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe His Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  165
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214I

<400>  165

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ile Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  166
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214K

<400>  166

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Lys Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  167
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214L

<400>  167

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Leu Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  168
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214M

<400>  168

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Met Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  169
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214N

<400>  169

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Asn Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  170
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214P

<400>  170

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Pro Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  171
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214Q

<400>  171

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Gln Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  172
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214R

<400>  172

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Arg Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  173
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214S

<400>  173

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  174
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214T

<400>  174

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Thr Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  175
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214V

<400>  175

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Val Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  176
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214W

<400>  176

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Trp Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  177
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214Y

<400>  177

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Tyr Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  178
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53A

<400>  178

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  179
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53C

<400>  179

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Cys Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  180
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53D

<400>  180

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Asp Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  181
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53E

<400>  181

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Glu Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  182
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53F

<400>  182

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Phe Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  183
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53G

<400>  183

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Gly Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  184
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53H

<400>  184

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met His Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  185
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53I

<400>  185

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ile Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  186
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53K

<400>  186

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Lys Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  187
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53L

<400>  187

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Leu Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  188
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53M

<400>  188

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Met Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  189
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53N

<400>  189

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Asn Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  190
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53P

<400>  190

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Pro Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  191
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q

<400>  191

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Gln Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  192
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53R

<400>  192

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Arg Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  193
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53S

<400>  193

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ser Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  194
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T

<400>  194

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  195
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53V

<400>  195

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Val Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  196
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53W

<400>  196

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Trp Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  197
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Y

<400>  197

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Tyr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  198
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V294N

<400>  198

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Asn Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  199
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L219F, V294N

<400>  199

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Phe Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Asn Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  200
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L219F, Q295A

<400>  200

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Phe Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Ala Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  201
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V294N, Q295A

<400>  201

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Asn Ala Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  202
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L219F

<400>  202

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Phe Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  203
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A

<400>  203

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Ala Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  204
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V294A

<400>  204

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Ala Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  205
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, V294A

<400>  205

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Ala Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Ala Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  206
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, V294A

<400>  206

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Ala Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  207
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, Q161S

<400>  207

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Ala Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  208
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177Y

<400>  208

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Tyr Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  209
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288H

<400>  209

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala His 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  210
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, Y288H

<400>  210

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Gln Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala His 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  211
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177Y, Y288H

<400>  211

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Tyr Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala His 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  212
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, S177Y

<400>  212

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Gln Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Tyr Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  213
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant D166E

<400>  213

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Glu Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  214
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q295W

<400>  214

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Trp Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  215
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant D166E, Q295W

<400>  215

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Glu Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Trp Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  216
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T D166E

<400>  216

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Glu Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  217
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123H

<400>  217

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala His Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  218
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L174V

<400>  218

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Val Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  219
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177E

<400>  219

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Glu Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  220
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123H, S177E

<400>  220

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala His Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Glu Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  221
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L174V, S177E

<400>  221

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Val Tyr Phe 
                165                 170                 175     


Glu Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  222
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123H, L174V

<400>  222

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala His Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Val Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  223
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M106E

<400>  223

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Glu Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  224
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S

<400>  224

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  225
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M106E, Q161S

<400>  225

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Glu Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  226
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, M106E

<400>  226

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Glu Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  227
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q38G

<400>  227

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gly Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  228
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant D166E

<400>  228

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Glu Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  229
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295A

<400>  229

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Ala Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  230
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q38G, Q295A

<400>  230

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gly Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Ala Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  231
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant D166E, Q295A

<400>  231

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Glu Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Ala Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  232
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q38G, D166E

<400>  232

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gly Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Glu Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  233
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118N

<400>  233

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Asn Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  234
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214F

<400>  234

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Phe Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  235
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, S214F

<400>  235

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Phe Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  236
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118N, S214F

<400>  236

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Asn Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Phe Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  237
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, K118N

<400>  237

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Asn Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  238
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177W

<400>  238

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Trp Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  239
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L219F

<400>  239

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Phe Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  240
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177W, L219F

<400>  240

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Trp Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Phe Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  241
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, S177W

<400>  241

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Gln Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Trp Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  242
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, L219F

<400>  242

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Gln Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Phe Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  243
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A108G

<400>  243

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Gly Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  244
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant G205M

<400>  244

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Met Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  245
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A108G, G205M

<400>  245

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Gly Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Met Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  246
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, G205M

<400>  246

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Met Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  247
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A108G, Q161S

<400>  247

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Gly Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  248
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118Q

<400>  248

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Gln Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  249
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M162A

<400>  249

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Ala Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  250
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y283L

<400>  250

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Leu Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  251
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V271E

<400>  251

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Glu Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  252
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K119D

<400>  252

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Asp Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  253
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L274V

<400>  253

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Val Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  254
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A232S

<400>  254

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ser Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  255
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161A

<400>  255

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ala Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  256
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant G205L

<400>  256

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Leu Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  257
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214G

<400>  257

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Gly Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  258
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y216A

<400>  258

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Ala Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  259
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant N173D

<400>  259

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asp Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  260
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123W

<400>  260

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Trp Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  261
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118N

<400>  261

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Asn Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  262
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant R228Q

<400>  262

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Gln Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  263
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161W

<400>  263

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Trp Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  264
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V294F

<400>  264

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Phe Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  265
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214R

<400>  265

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Arg Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  266
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant G286E

<400>  266

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Glu Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  267
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant R228E

<400>  267

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Glu Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  268
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant C230S

<400>  268

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Ser Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  269
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant D227E

<400>  269

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Glu Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  270
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M162F

<400>  270

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Phe Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  271
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123A

<400>  271

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Ala Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  272
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, V294A

<400>  272

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Ala Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  273
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S, V294A

<400>  273

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Ala Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  274
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S, V294N

<400>  274

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Asn Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  275
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q295A

<400>  275

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Ala Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  276
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, V294A, Q295A

<400>  276

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Ala Ala Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  277
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S, Q295A

<400>  277

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Ala Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  278
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, V294A, Q295A

<400>  278

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Ala Ala Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  279
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S, V294A, Q295A

<400>  279

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Ala Ala Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  280
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S, V294N, Q295A

<400>  280

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Asn Ala Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  281
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q295W

<400>  281

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Trp Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  282
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, V294A, Q295W

<400>  282

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Ala Trp Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  283
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S, Q295W

<400>  283

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Trp Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  284
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, V294A, Q295W

<400>  284

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Ala Trp Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  285
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S, V294A, Q295W

<400>  285

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Ala Trp Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  286
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S, V294N, Q295W

<400>  286

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Asn Trp Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  287
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177W, Q295A

<400>  287

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Trp Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Ala Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  288
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177W, S214R

<400>  288

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Trp Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Arg Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  289
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, S177W

<400>  289

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Trp Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  290
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, S177W

<400>  290

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Trp Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  291
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, Q295L

<400>  291

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Ala Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Leu Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  292
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, S214R

<400>  292

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Ala Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Arg Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  293
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q295F

<400>  293

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Phe Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  294
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, S214R

<400>  294

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Arg Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  295
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S

<400>  295

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Thr Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  296
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, Q295F

<400>  296

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Phe Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  297
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, Q295L

<400>  297

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Leu Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  298
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, S214R

<400>  298

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Arg Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  299
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214R, Q295F

<400>  299

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Val Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Arg Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Phe Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  300
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, Q161S

<400>  300

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Ala Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Ser Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Tyr 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  301
<211>  927
<212>  DNA
<213>  Unknown

<220>
<223>  Streptomyces sp. strain CL190

<400>  301
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  302
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A108G, Q295N, L298Q

<400>  302
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cggtattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttaaccgtgg ccagctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  303
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K119A, Q161S, S177E

<400>  303
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaagcgacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcga ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  304
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49S, M162A, Y283L

<400>  304
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcagcttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

caggcgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtacctga agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  305
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118Q, Q161W, S214F

<400>  305
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt ccagaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

tggatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttt tcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  306
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161A, S214G, V294F

<400>  306
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

gcgatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttg gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgact tccaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  307
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123W, S177W, G286E

<400>  307
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgcttggt ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttctg ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctggaggc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  308
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118N, N173D, Q295N

<400>  308
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caacaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtagacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttaaccgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  309
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K119A, Q161A, R228Q

<400>  309
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaagcgacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

gcgatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccagctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  310
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q38G, E112D, F123H

<400>  310
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tggtgacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgacgtca cgggcggttt caaaaagacc      360

tatgctcact ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  311
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, S177Y, C209G

<400>  311
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgcgttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcta cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attcggtaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  312
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118N, K119A, V271E

<400>  312
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caacgcgacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gagtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  313
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L174V, C230N, L298Q

<400>  313
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacg tttatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgaac tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg ccagctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  314
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K119D, S177E, L219F

<400>  314
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaagacacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcga ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccttcaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  315
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53E, E112G, R228E

<400>  315
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggaga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtggtgtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga cgagctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  316
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53E, F213M, R228Q

<400>  316
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggaga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcatga gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccagctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  317
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, S177W, L219F

<400>  317
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgcaga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttctg ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccttcaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  318
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123A, Y283L

<400>  318
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcctgttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctgcgt ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtacctga agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  319
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant C25V, Y121W, M162F

<400>  319
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg ctgttgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tgggctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagttcacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  320
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49L, S214R, V271E

<400>  320
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcctgttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttc gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gagtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  321
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant D227E, C230N, Q295W

<400>  321
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga gcgcctgaac tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg tttggcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  322
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant G205L, Y216A, C230S

<400>  322
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgctgctgaa attctgcaag cgtagcttta gcgttgcgcc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgagc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  323
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, V294F, L298W

<400>  323
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgact tccaacgtgg ctggctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  324
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L219F, Y283L, L298W

<400>  324
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccttcaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtacctga agctgggcgc gtactaccac attaccgacg ttcaacgtgg ctggctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  325
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A17T, F213M, S214R

<400>  325
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaaac cgccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcatgc gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  326
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L219F, V294N, Q295A

<400>  326
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccttcaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgaca acgcgcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  327
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53E, A108G, K118N

<400>  327
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggaga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cggtattgat ggtgaggtca cgggcggttt caacaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  328
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q38G, E112G, M162F

<400>  328
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tggtgacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtggtgtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagttcacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  329
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, D166E, Q295W

<400>  329
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggagta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg tttggcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  330
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L174V, C230S, V294A

<400>  330
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacg tttatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgagc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg cgcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  331
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M106E, G286E, L298W

<400>  331
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcgagtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctggaggc gtactaccac attaccgacg ttcaacgtgg ctggctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  332
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant D227E, R228E, L298Q

<400>  332
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ggagctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg ccagctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  333
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M162A, C209G, Y288H

<400>  333
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

caggcgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attcggtaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gcactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  334
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A17T, C25V, E112G

<400>  334
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaaac cgccggtttg       60

ctgggtgtgg ctgttgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtggtgtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  335
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, Y121W, A232S

<400>  335
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgcaga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tgggctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttagcgtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  336
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, S177Y, Y288H

<400>  336
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgcaga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcta cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gcactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  337
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123H, L274V, L298A

<400>  337
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctcact ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtg ttaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cgcgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  338
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177W, S214F, Y283L

<400>  338
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttctg ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttt tcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtacctga agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  339
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53E, G205M, L274V

<400>  339
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggaga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgatgctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtg ttaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  340
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, K118N, S214F

<400>  340
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caacaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttt tcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  341
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177E, S214R, R228E

<400>  341
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcga ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttc gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga cgagctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  342
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118Q, S177Y, D227E

<400>  342
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt ccagaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcta cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga gcgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  343
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant D166E, S177Y, S214F

<400>  343
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggagta caagaaacgc caggtaaacc tgtatttcta cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttt tcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  344
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161A, M162F, Q295A

<400>  344
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

gcgttcacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttgcgcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  345
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53E, Q161A, V294N

<400>  345
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggaga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

gcgatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgaca accaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  346
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A108G, F123W, V294A

<400>  346
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cggtattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgcttggt ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg cgcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  347
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, G205L, Q295N

<400>  347
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgcaga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgctgctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttaaccgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  348
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118Q, F123A, R228E

<400>  348
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt ccagaagacc      360

tatgctgcgt ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga cgagctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  349
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49L, E112D, G286E

<400>  349
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcctgttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgacgtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctggaggc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  350
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant G205L, R228E, C230N

<400>  350
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgctgctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga cgagctgaac tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  351
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M162A, D227E, C230S

<400>  351
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

caggcgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga gcgcctgagc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  352
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49L, K119D, G205M

<400>  352
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcctgttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaagacacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgatgctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  353
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K119A, S214G, L298A

<400>  353
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaagcgacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttg gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cgcgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  354
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q38G, Y216A, A232S

<400>  354
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tggtgacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgttgcgcc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttagcgtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  355
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161A, V271E, Q295W

<400>  355
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

gcgatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gagtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg tttggcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  356
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49S, Y216A, V294N

<400>  356
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcagcttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgttgcgcc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgaca accaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  357
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123A, M162F, S214G

<400>  357
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctgcgt ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagttcacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttg gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  358
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant C25V, A232S, V271E

<400>  358
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg ctgttgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttagcgtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gagtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  359
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118Q, L174V, R228Q

<400>  359
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt ccagaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacg tttatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccagctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  360
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49S, S214G, V294A

<400>  360
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcagcttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttg gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg cgcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  361
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant E112D, K119A, N173D

<400>  361
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgacgtca cgggcggttt caaagcgacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtagacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  362
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123W, Q161W, D227E

<400>  362
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgcttggt ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

tggatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga gcgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  363
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, N173D, S214R

<400>  363
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtagacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttc gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  364
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123W, M162F, C209G

<400>  364
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgcttggt ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagttcacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attcggtaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  365
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M106E, M162A, Y216A

<400>  365
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcgagtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

caggcgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgttgcgcc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  366
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, Q161S, V294A

<400>  366
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgcgttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg cgcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  367
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant C25V, F213M, Y216A

<400>  367
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg ctgttgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcatga gcgttgcgcc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  368
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A108G, K119D, L298A

<400>  368
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cggtattgat ggtgaggtca cgggcggttt caaagacacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cgcgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  369
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118N, C209G, R228Q

<400>  369
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caacaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attcggtaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccagctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  370
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, M106E, Q161S

<400>  370
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcgagtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  371
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q38G, D166E, Q295A

<400>  371
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tggtgacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggagta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttgcgcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  372
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M106E, G205L, C209G

<400>  372
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcgagtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgctgctgaa attcggtaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  373
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant E112G, G205M, L298W

<400>  373
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtggtgtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgatgctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg ctggctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  374
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49S, K118Q, S177E

<400>  374
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcagcttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt ccagaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcga ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  375
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M106E, Y121W, D166E

<400>  375
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcgagtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tgggctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggagta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  376
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, L274V, Q295A

<400>  376
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgcaga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtg ttaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttgcgcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  377
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, F123A, Y288H

<400>  377
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgcgttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctgcgt ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gcactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  378
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant C25V, F123H, Q295N

<400>  378
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg ctgttgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctcact ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttaaccgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  379
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K119D, Q161W, L298Q

<400>  379
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaagacacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

tggatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg ccagctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  380
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, Y121W, C230S

<400>  380
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgcgttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tgggctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgagc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  381
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M162A, N173D, S214F

<400>  381
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

caggcgacca gcatggatta caagaaacgc caggtagacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttt tcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  382
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49L, D166E, L274V

<400>  382
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcctgttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggagta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtg ttaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  383
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49S, K119D, F213M

<400>  383
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcagcttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaagacacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcatga gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  384
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant E112D, L219F, V294F

<400>  384
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgacgtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccttcaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgact tccaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  385
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123H, L174V, S177E

<400>  385
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctcact ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacg tttatttcga ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  386
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant E112G, F123H, S214G

<400>  386
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtggtgtca cgggcggttt caaaaagacc      360

tatgctcact ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttg gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  387
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123A, S177W, G205L

<400>  387
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctgcgt ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttctg ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgctgctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  388
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177W, Y288H, V294N

<400>  388
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttctg ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gcactaccac attaccgaca accaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  389
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A108G, Q161S, G205M

<400>  389
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cggtattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgatgctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  390
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y121W, S177Y, G286E

<400>  390
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tgggctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcta cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctggaggc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  391
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A17T, F123W, L298A

<400>  391
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaaac cgccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgcttggt ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cgcgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  392
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant N173D, F213M, V294F

<400>  392
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtagacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcatga gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgact tccaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  393
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, E112D, G205M

<400>  393
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgacgtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgatgctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  394
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q38G, L174V, S214R

<400>  394
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tggtgacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacg tttatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttc gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  395
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A17T, V49A, C230N

<400>  395
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaaac cgccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgcgttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgaac tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  396
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A17T, Q161W, A232S

<400>  396
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaaac cgccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

tggatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttagcgtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  397
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161W, R228Q, Q295W

<400>  397
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

tggatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccagctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg tttggcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  398
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288A

<400>  398
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc ggcataccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  399
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288C

<400>  399
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtgttaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  400
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288D

<400>  400
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc ggattaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  401
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288E

<400>  401
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc ggaataccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  402
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288F

<400>  402
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gttttaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  403
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288G

<400>  403
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gggttaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  404
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288H

<400>  404
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gcattaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  405
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288I

<400>  405
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gatttaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  406
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288K

<400>  406
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gaaataccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  407
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288L

<400>  407
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gctgtaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  408
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288M

<400>  408
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gatgtaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  409
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288N

<400>  409
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gaattaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  410
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288P

<400>  410
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gccgtaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  411
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288Q

<400>  411
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gcagtaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  412
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288R

<400>  412
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gcgttaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  413
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288S

<400>  413
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gagctaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  414
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288T

<400>  414
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gacctaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  415
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288V

<400>  415
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc ggtttaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  416
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288W

<400>  416
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtggtaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  417
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288Y

<400>  417
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtattaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  418
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161A

<400>  418
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

gcaatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  419
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161C

<400>  419
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

tgtatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  420
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161D

<400>  420
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

gatatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  421
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161E

<400>  421
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

gaaatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  422
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161F

<400>  422
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

tttatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  423
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161G

<400>  423
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

ggtatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  424
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161H

<400>  424
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

catatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  425
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161I

<400>  425
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

attatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  426
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161K

<400>  426
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

aaaatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  427
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161L

<400>  427
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

ctgatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  428
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161M

<400>  428
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

atgatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  429
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161N

<400>  429
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

aatatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  430
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161P

<400>  430
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

ccgatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  431
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161Q

<400>  431
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  432
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161R

<400>  432
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cgtatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  433
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S

<400>  433
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  434
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161T

<400>  434
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

accatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  435
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161V

<400>  435
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

gttatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  436
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161W

<400>  436
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

tggatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  437
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161Y

<400>  437
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

tatatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  438
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295A

<400>  438
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttgcacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  439
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295C

<400>  439
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg tttgtcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  440
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295D

<400>  440
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttgatcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  441
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295E

<400>  441
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttgaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  442
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295F

<400>  442
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg tttttcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  443
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295G

<400>  443
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttggtcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  444
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295H

<400>  444
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcatcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  445
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295I

<400>  445
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttattcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  446
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295K

<400>  446
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttaaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  447
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295L

<400>  447
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttctgcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  448
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295M

<400>  448
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttatgcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  449
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295N

<400>  449
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttaatcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  450
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295P

<400>  450
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttccgcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  451
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295Q

<400>  451
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcagcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  452
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295R

<400>  452
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcgtcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  453
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295S

<400>  453
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttagccgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  454
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295T

<400>  454
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttacccgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  455
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295V

<400>  455
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttgttcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  456
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295W

<400>  456
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg tttggcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  457
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295Y

<400>  457
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg tttatcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  458
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214A

<400>  458
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttg cagtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  459
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214C

<400>  459
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttt gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  460
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214D

<400>  460
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttg atgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  461
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214E

<400>  461
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttg aagtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  462
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214F

<400>  462
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttt ttgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  463
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214G

<400>  463
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttg gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  464
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214H

<400>  464
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttc atgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  465
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214I

<400>  465
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta ttgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  466
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214K

<400>  466
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta aagtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  467
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214L

<400>  467
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttc tggtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  468
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214M

<400>  468
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta tggtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  469
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214N

<400>  469
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta atgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  470
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214P

<400>  470
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttc cggtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  471
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214Q

<400>  471
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttc aggtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  472
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214R

<400>  472
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttc gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  473
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214S

<400>  473
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  474
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214T

<400>  474
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta ccgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  475
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214V

<400>  475
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttg ttgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  476
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214W

<400>  476
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttt gggtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  477
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214Y

<400>  477
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttt atgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  478
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53A

<400>  478
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcaa gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  479
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53C

<400>  479
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgtgta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  480
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53D

<400>  480
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggata gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  481
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53E

<400>  481
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggaaa gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  482
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53F

<400>  482
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgttta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  483
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53G

<400>  483
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgggta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  484
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53H

<400>  484
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgcata gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  485
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53I

<400>  485
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgatta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  486
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53K

<400>  486
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgaaaa gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  487
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53L

<400>  487
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgctga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  488
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53M

<400>  488
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgatga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  489
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53N

<400>  489
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgaata gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  490
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53P

<400>  490
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgccga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  491
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q

<400>  491
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgcaga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  492
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53R

<400>  492
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgcgta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  493
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53S

<400>  493
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgagca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  494
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T

<400>  494
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  495
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53V

<400>  495
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggtta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  496
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53W

<400>  496
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgtgga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  497
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Y

<400>  497
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgtata gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  498
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V294N

<400>  498
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgaca accaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  499
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L219F, V294N

<400>  499
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccttcaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgaca accaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  500
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L219F, Q295A

<400>  500
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccttcaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttgcgcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  501
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V294N, Q295A

<400>  501
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgaca acgcgcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  502
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L219F

<400>  502
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccttcaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  503
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A

<400>  503
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgcgttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  504
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V294A

<400>  504
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg cgcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  505
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, V294A

<400>  505
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgcgttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg cgcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  506
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, V294A

<400>  506
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg cgcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  507
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, Q161S

<400>  507
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgcgttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  508
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177Y

<400>  508
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcta cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  509
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y288H

<400>  509
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gcactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  510
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, Y288H

<400>  510
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgcaga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gcactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  511
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177Y, Y288H

<400>  511
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcta cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gcactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  512
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, S177Y

<400>  512
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgcaga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcta cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  513
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant D166E

<400>  513
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggagta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  514
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q295W

<400>  514
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg tttggcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  515
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant D166E, Q295W

<400>  515
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggagta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg tttggcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  516
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T D166E

<400>  516
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggagta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  517
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123H

<400>  517
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctcact ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  518
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L174V

<400>  518
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacg tttatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  519
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177E

<400>  519
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcga ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  520
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123H, S177E

<400>  520
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctcact ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcga ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  521
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L174V, S177E

<400>  521
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacg tttatttcga ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  522
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123H, L174V

<400>  522
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctcact ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacg tttatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  523
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M106E

<400>  523
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcgagtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  524
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S

<400>  524
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  525
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M106E, Q161S

<400>  525
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcgagtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  526
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, M106E

<400>  526
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcgagtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  527
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q38G

<400>  527
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tggtgacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  528
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant D166E

<400>  528
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggagta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  529
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q295A

<400>  529
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttgcgcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  530
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q38G, Q295A

<400>  530
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tggtgacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttgcgcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  531
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant D166E, Q295A

<400>  531
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggagta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttgcgcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  532
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q38G, D166E

<400>  532
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tggtgacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggagta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  533
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118N

<400>  533
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caacaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  534
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214F

<400>  534
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttt tcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  535
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, S214F

<400>  535
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttt tcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  536
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118N, S214F

<400>  536
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caacaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttt tcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  537
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, K118N

<400>  537
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caacaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  538
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177W

<400>  538
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttctg ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  539
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L219F

<400>  539
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccttcaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  540
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177W, L219F

<400>  540
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttctg ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccttcaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  541
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, S177W

<400>  541
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgcaga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttctg ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  542
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53Q, L219F

<400>  542
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgcaga gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccttcaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  543
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A108G

<400>  543
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cggtattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  544
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant G205M

<400>  544
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgatgctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  545
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A108G, G205M

<400>  545
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cggtattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgatgctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  546
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, G205M

<400>  546
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgatgctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  547
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A108G, Q161S

<400>  547
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cggtattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  548
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118Q

<400>  548
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt ccagaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  549
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M162A

<400>  549
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

caggcgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  550
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y283L

<400>  550
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtacctga agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  551
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V271E

<400>  551
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gagtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  552
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K119D

<400>  552
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaagacacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  553
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant L274V

<400>  553
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtg ttaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  554
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A232S

<400>  554
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttagcgtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  555
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161A

<400>  555
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

gcgatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  556
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant G205L

<400>  556
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgctgctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  557
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214G

<400>  557
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttg gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  558
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Y216A

<400>  558
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgttgcgcc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  559
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant N173D

<400>  559
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtagacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  560
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123W

<400>  560
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgcttggt ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  561
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant K118N

<400>  561
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caacaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  562
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant R228Q

<400>  562
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccagctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  563
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161W

<400>  563
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

tggatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  564
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V294F

<400>  564
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgact tccaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  565
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214R

<400>  565
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttc gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  566
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant G286E

<400>  566
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctggaggc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  567
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant R228E

<400>  567
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga cgagctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  568
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant C230S

<400>  568
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgagc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  569
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant D227E

<400>  569
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga gcgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  570
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant M162F

<400>  570
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagttcacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  571
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant F123A

<400>  571
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctgcgt ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  572
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, V294A

<400>  572
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg cgcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  573
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S, V294A

<400>  573
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg cgcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  574
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S, V294Nqq

<400>  574
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgaca accaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  575
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q295A

<400>  575
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttgcacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  576
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, V294A, Q295A

<400>  576
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg cggcacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  577
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S, Q295A

<400>  577
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttgcacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  578
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, V294A, Q295A

<400>  578
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg cggcacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  579
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S, V294A, Q295A

<400>  579
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg cggcacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  580
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S, V294N, Q295A

<400>  580
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgaca acgcacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  581
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q295W

<400>  581
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg tttggcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  582
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, V294A, Q295W

<400>  582
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg cgtggcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  583
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S, Q295W

<400>  583
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg tttggcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  584
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, V294A, Q295W

<400>  584
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg cgtggcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  585
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S, V294A, Q295W

<400>  585
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg cgtggcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  586
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q161S, V294N, Q295W

<400>  586
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgaca actggcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  587
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177W, Q295

<400>  587
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttctg ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttgcacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  588
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S177W, S214R

<400>  588
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttctg ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttc gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  589
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, S177W

<400>  589
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttctg ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  590
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, S177W

<400>  590
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttctg ggagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  591
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, Q295L

<400>  591
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgcgttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttctgcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  592
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, S214R

<400>  592
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgcgttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttc gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  593
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, Q295F

<400>  593
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg tttttcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  594
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, S214R

<400>  594
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttc gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  595
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant A53T, A161S

<400>  595
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatgacca gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  596
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, Q295F

<400>  596
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg tttttcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  597
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, Q295L

<400>  597
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttctgcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  598
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant Q161S, S214R

<400>  598
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttc gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  599
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant S214R, Q295F

<400>  599
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgttttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

cagatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagctttc gtgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg tttttcgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  600
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF2 (NphB) recombinant mutant V49A, Q161S

<400>  600
atgagcgaag cggcggacgt cgaacgtgtt tatgcagcga tggaagaagc agccggtttg       60

ctgggtgtgg cttgtgcgcg tgataagatt tatccactgc tgagcacttt tcaagacacg      120

ctggtcgagg gtggctcggt ggtcgcgttt agcatggcta gcggtcgcca cagcaccgag      180

ctggacttct ccatctccgt cccgaccagc cacggtgatc cttacgccac cgttgtggag      240

aaaggtctgt tcccggcgac cggccacccg gtcgacgatc tgctggccga cacgcaaaag      300

catctgccgg tgagcatgtt cgcaattgat ggtgaggtca cgggcggttt caaaaagacc      360

tatgctttct ttccgaccga caatatgcct ggcgttgcgg agctgagcgc gatcccgtct      420

atgccgccag cggtggccga gaatgcagag ctgttcgctc gttacggtct ggataaggtg      480

agcatgacca gcatggatta caagaaacgc caggtaaacc tgtatttcag cgagctgtct      540

gcacagacgc tcgaagcgga gtccgttttg gcactggtgc gtgagttggg tctgcacgtt      600

ccgaatgaat tgggtctgaa attctgcaag cgtagcttta gcgtttaccc gaccctgaac      660

tgggaaaccg gcaagattga ccgcctgtgc tttgcggtga tcagcaacga tccgacgctg      720

gttccgagca gcgatgaagg cgacatcgag aaatttcata actatgcaac taaagcgccg      780

tacgcctatg tcggtgagaa gcgcacgctg gtgtacggtc tgaccctgag cccgaaagaa      840

gagtactata agctgggcgc gtactaccac attaccgacg ttcaacgtgg cctgctgaaa      900

gcgtttgact ctttggaaga ttaatga                                          927


<210>  601
<211>  879
<212>  DNA
<213>  Myxococcus stipitatus

<400>  601
atgccggcgc tgtccttggg tcttgaacgt ttgtgtgcag acgttgaggc cgcagcagcg       60

ctggcaggcg cgagcttcag ccgcgaggtg acgcgtaatg ttctgaaaag ctacccgcgc      120

tttttcacca gcagcgcggt cagcttccgc accagcacgc gtaaaccgga gaagcgtgag      180

ctgaacgtcc gctttgttga actggaaacc cctgaggatc cgcacgctgt tgccctggca      240

gagggcctga ttcaccgcag cggccacccg attgacgacc tgtttgagca agtccaacgt      300

aacgtgccga ttctgggtta tggtctggac ttcggtgtgg cttatggtgt ggaaaagatc      360

tggccgtttt tcccgcatcg ccctcagcca ctggaagttc tgcgtacgct gccgtccttg      420

ccgcaaagcg tgcaggcgca tagcggcttt ctggttgaac acgatctgac ggatctcagc      480

ctgtttgcgc tggactaccg ttctcgctcg gttaatctgt atttcatgtg ccgtccgggt      540

cactttagca ccgcacagtt ggcggatctg ctgggcggtc tgggttttga aagcccgggt      600

gaagaactgt tggagcactg tacccgtgcg gtcccgatct acttcacctt ccgttgggac      660

cgtccgcgta ttgagcgtgt ctgcttcggc gtcatcgcgc caggtccggg tctgttaccg      720

acccatctgc acccgatcat cggccagttc gctgccggtg tgccgtttgc cactgagcgt      780

cgtaacttca tttacagcgt taccgtgtcc cgcgaggaaa ccttcattaa gatcgagaat      840

gattactctg gcactatgac ggcgctgatg caagtgttt                             879


<210>  602
<211>  293
<212>  PRT
<213>  Myxococcus stipitatus

<400>  602

Met Pro Ala Leu Ser Leu Gly Leu Glu Arg Leu Cys Ala Asp Val Glu 
1               5                   10                  15      


Ala Ala Ala Ala Leu Ala Gly Ala Ser Phe Ser Arg Glu Val Thr Arg 
            20                  25                  30          


Asn Val Leu Lys Ser Tyr Pro Arg Phe Phe Thr Ser Ser Ala Val Ser 
        35                  40                  45              


Phe Arg Thr Ser Thr Arg Lys Pro Glu Lys Arg Glu Leu Asn Val Arg 
    50                  55                  60                  


Phe Val Glu Leu Glu Thr Pro Glu Asp Pro His Ala Val Ala Leu Ala 
65                  70                  75                  80  


Glu Gly Leu Ile His Arg Ser Gly His Pro Ile Asp Asp Leu Phe Glu 
                85                  90                  95      


Gln Val Gln Arg Asn Val Pro Ile Leu Gly Tyr Gly Leu Asp Phe Gly 
            100                 105                 110         


Val Ala Tyr Gly Val Glu Lys Ile Trp Pro Phe Phe Pro His Arg Pro 
        115                 120                 125             


Gln Pro Leu Glu Val Leu Arg Thr Leu Pro Ser Leu Pro Gln Ser Val 
    130                 135                 140                 


Gln Ala His Ser Gly Phe Leu Val Glu His Asp Leu Thr Asp Leu Ser 
145                 150                 155                 160 


Leu Phe Ala Leu Asp Tyr Arg Ser Arg Ser Val Asn Leu Tyr Phe Met 
                165                 170                 175     


Cys Arg Pro Gly His Phe Ser Thr Ala Gln Leu Ala Asp Leu Leu Gly 
            180                 185                 190         


Gly Leu Gly Phe Glu Ser Pro Gly Glu Glu Leu Leu Glu His Cys Thr 
        195                 200                 205             


Arg Ala Val Pro Ile Tyr Phe Thr Phe Arg Trp Asp Arg Pro Arg Ile 
    210                 215                 220                 


Glu Arg Val Cys Phe Gly Val Ile Ala Pro Gly Pro Gly Leu Leu Pro 
225                 230                 235                 240 


Thr His Leu His Pro Ile Ile Gly Gln Phe Ala Ala Gly Val Pro Phe 
                245                 250                 255     


Ala Thr Glu Arg Arg Asn Phe Ile Tyr Ser Val Thr Val Ser Arg Glu 
            260                 265                 270         


Glu Thr Phe Ile Lys Ile Glu Asn Asp Tyr Ser Gly Thr Met Thr Ala 
        275                 280                 285             


Leu Met Gln Val Phe 
    290             


<210>  603
<211>  1260
<212>  DNA
<213>  Aspergillus terreus

<400>  603
atgggaccac tagggtcccg tccgtggcag atcctgtcac aggctttggg tttcccgaac       60

tatgaccagg agctttggtg gcagaatacc gcagagacat tgaatcgtgt attagagcaa      120

tgcgattact cagtacacct tcagtataag tacctggcct tctatcacaa gtatatcctg      180

ccctcactag gaccttttag gagaccaggt gtcgagccag agtacatcag cggattatca      240

catggcggcc acccactaga aatctccgta aaaatagata agtcaaaaac gatttgcagg      300

ttgggccttc aagctatagg gccgctggca gggacagcta gggacccact gaactcattc      360

ggggacaggg aactgttgaa gaatctagcc actcttctac cccatgtaga cctacgtcta      420

ttcgatcact ttaacgccca agtgggttta gatagggcgc agtgcgcagt ggcaactacg      480

aaacttatta aagaatcaca taatattgtt tgtacaagtc tggatttaaa ggacggcgaa      540

gtgataccga aagtttactt ctcaacgatc cccaagggac tggtcacgga aacgcccctt      600

ttcgacctta cttttgctgc cattgagcaa atggaagtgt atcataagga cgcgccgctg      660

aggacagcct tatcatcctt gaaggacttt ttgcgtccgc gtgtcccgac ggacgcttca      720

attaccccgc ctctaactgg gttaataggg gtcgattgca tcgatccaat gctaagccgt      780

ttgaaggtat atttagccac cttcaggatg gacctgagcc ttatccgtga ttattggact      840

cttggcggac ttttgacgga tgctgggact atgaaagggt tagaaatggt tgagactctg      900

gccaagaccc taaaactagg agatgaagca tgcgaaaccc ttgatgcaga acgtttgccc      960

ttcggaatta attatgctat gaagcccgga accgccgaat tggcaccccc acagatatac     1020

tttccgttgc taggaatcaa cgatggattc attgcagacg ccttagtcga gttttttcag     1080

tacatgggct gggaggatca ggccaataga tataaagatg aattgaaggc aaagttcccc     1140

aatgtagata tatctcaaac gaagaacgtt caccgttggc taggggtcgc atatagcgaa     1200

acgaaaggtc caagtatgaa catatattac gacgtcgtcg caggcaacgt ggcgagggtc     1260


<210>  604
<211>  420
<212>  PRT
<213>  Aspergillus terreus

<400>  604

Met Gly Pro Leu Gly Ser Arg Pro Trp Gln Ile Leu Ser Gln Ala Leu 
1               5                   10                  15      


Gly Phe Pro Asn Tyr Asp Gln Glu Leu Trp Trp Gln Asn Thr Ala Glu 
            20                  25                  30          


Thr Leu Asn Arg Val Leu Glu Gln Cys Asp Tyr Ser Val His Leu Gln 
        35                  40                  45              


Tyr Lys Tyr Leu Ala Phe Tyr His Lys Tyr Ile Leu Pro Ser Leu Gly 
    50                  55                  60                  


Pro Phe Arg Arg Pro Gly Val Glu Pro Glu Tyr Ile Ser Gly Leu Ser 
65                  70                  75                  80  


His Gly Gly His Pro Leu Glu Ile Ser Val Lys Ile Asp Lys Ser Lys 
                85                  90                  95      


Thr Ile Cys Arg Leu Gly Leu Gln Ala Ile Gly Pro Leu Ala Gly Thr 
            100                 105                 110         


Ala Arg Asp Pro Leu Asn Ser Phe Gly Asp Arg Glu Leu Leu Lys Asn 
        115                 120                 125             


Leu Ala Thr Leu Leu Pro His Val Asp Leu Arg Leu Phe Asp His Phe 
    130                 135                 140                 


Asn Ala Gln Val Gly Leu Asp Arg Ala Gln Cys Ala Val Ala Thr Thr 
145                 150                 155                 160 


Lys Leu Ile Lys Glu Ser His Asn Ile Val Cys Thr Ser Leu Asp Leu 
                165                 170                 175     


Lys Asp Gly Glu Val Ile Pro Lys Val Tyr Phe Ser Thr Ile Pro Lys 
            180                 185                 190         


Gly Leu Val Thr Glu Thr Pro Leu Phe Asp Leu Thr Phe Ala Ala Ile 
        195                 200                 205             


Glu Gln Met Glu Val Tyr His Lys Asp Ala Pro Leu Arg Thr Ala Leu 
    210                 215                 220                 


Ser Ser Leu Lys Asp Phe Leu Arg Pro Arg Val Pro Thr Asp Ala Ser 
225                 230                 235                 240 


Ile Thr Pro Pro Leu Thr Gly Leu Ile Gly Val Asp Cys Ile Asp Pro 
                245                 250                 255     


Met Leu Ser Arg Leu Lys Val Tyr Leu Ala Thr Phe Arg Met Asp Leu 
            260                 265                 270         


Ser Leu Ile Arg Asp Tyr Trp Thr Leu Gly Gly Leu Leu Thr Asp Ala 
        275                 280                 285             


Gly Thr Met Lys Gly Leu Glu Met Val Glu Thr Leu Ala Lys Thr Leu 
    290                 295                 300                 


Lys Leu Gly Asp Glu Ala Cys Glu Thr Leu Asp Ala Glu Arg Leu Pro 
305                 310                 315                 320 


Phe Gly Ile Asn Tyr Ala Met Lys Pro Gly Thr Ala Glu Leu Ala Pro 
                325                 330                 335     


Pro Gln Ile Tyr Phe Pro Leu Leu Gly Ile Asn Asp Gly Phe Ile Ala 
            340                 345                 350         


Asp Ala Leu Val Glu Phe Phe Gln Tyr Met Gly Trp Glu Asp Gln Ala 
        355                 360                 365             


Asn Arg Tyr Lys Asp Glu Leu Lys Ala Lys Phe Pro Asn Val Asp Ile 
    370                 375                 380                 


Ser Gln Thr Lys Asn Val His Arg Trp Leu Gly Val Ala Tyr Ser Glu 
385                 390                 395                 400 


Thr Lys Gly Pro Ser Met Asn Ile Tyr Tyr Asp Val Val Ala Gly Asn 
                405                 410                 415     


Val Ala Arg Val 
            420 


