                         SEQUENCE LISTING

<110>  The Trustees of the University of Pennsylvania
       O'Rourke, Donald
       Zev, Binder
       Milone, Michael
       Thokala, Rahika
 
<120>  Compositions and Methods Comprising a High Affinity Chimeric 
       Antigen Receptor with Cross Reactivity to Clinically Relevant 
       EGFR Mutated Proteins

<130>  046483-7244WO1(02264)

<150>  62/833,456
<151>  2019-04-12

<150>  62/892,343
<151>  2019-08-27

<160>  86    

<170>  PatentIn version 3.5

<210>  1
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  806 scFv

<400>  1
gatgtccagc tgcaagagtc tggccctagc ctggtcaagc ctagccagag cctgagcctg       60

acatgtaccg tgaccggcta cagcatcacc agcgacttcg cctggaactg gatcagacag      120

ttccccggca acaagctgga atggatgggc tacatcagct acagcggcaa cacccggtac      180

aaccccagcc tgaagtcccg gatctccatc accagagaca ccagcaagaa ccagttcttc      240

ctgcagctga acagcgtgac catcgaggac accgccacct actactgtgt gacagccggc      300

agaggcttcc cttattgggg acagggaacc ctggtcacag tgtctgctgg tggcggagga      360

tctggcggag gcggatcttc tggcggtggc tctgatatcc tgatgacaca gagccccagc      420

agcatgtctg tgtccctggg cgataccgtg tccatcacct gtcacagcag ccaggacatc      480

aacagcaaca tcggctggct gcagcagagg cctggcaagt cttttaaggg cctgatctac      540

cacggcacca acctggatga tgaggtgccc agcagatttt ccggctctgg aagcggagcc      600

gactactccc tgacaatcag cagcctggaa agcgaggact tcgccgatta ctactgcgtg      660

cagtacgccc agtttccttg gacctttgga ggcggcacaa agctggaaat caagcgg         717


<210>  2
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  806 scFv

<400>  2

Asp Val Gln Leu Gln Glu Ser Gly Pro Ser Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp 
            20                  25                  30          


Phe Ala Trp Asn Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp 
        35                  40                  45              


Met Gly Tyr Ile Ser Tyr Ser Gly Asn Thr Arg Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln Phe Phe 
65                  70                  75                  80  


Leu Gln Leu Asn Ser Val Thr Ile Glu Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Val Thr Ala Gly Arg Gly Phe Pro Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Gly 
        115                 120                 125             


Gly Gly Ser Asp Ile Leu Met Thr Gln Ser Pro Ser Ser Met Ser Val 
    130                 135                 140                 


Ser Leu Gly Asp Thr Val Ser Ile Thr Cys His Ser Ser Gln Asp Ile 
145                 150                 155                 160 


Asn Ser Asn Ile Gly Trp Leu Gln Gln Arg Pro Gly Lys Ser Phe Lys 
                165                 170                 175     


Gly Leu Ile Tyr His Gly Thr Asn Leu Asp Asp Glu Val Pro Ser Arg 
            180                 185                 190         


Phe Ser Gly Ser Gly Ser Gly Ala Asp Tyr Ser Leu Thr Ile Ser Ser 
        195                 200                 205             


Leu Glu Ser Glu Asp Phe Ala Asp Tyr Tyr Cys Val Gln Tyr Ala Gln 
    210                 215                 220                 


Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
225                 230                 235                 


<210>  3
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  806 VL

<400>  3

Asp Ile Leu Met Thr Gln Ser Pro Ser Ser Met Ser Val Ser Leu Gly 
1               5                   10                  15      


Asp Thr Val Ser Ile Thr Cys His Ser Ser Gln Asp Ile Asn Ser Asn 
            20                  25                  30          


Ile Gly Trp Leu Gln Gln Arg Pro Gly Lys Ser Phe Lys Gly Leu Ile 
        35                  40                  45              


Tyr His Gly Thr Asn Leu Asp Asp Glu Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Ala Asp Tyr Ser Leu Thr Ile Ser Ser Leu Glu Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Asp Tyr Tyr Cys Val Gln Tyr Ala Gln Phe Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105             


<210>  4
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  806 VH

<400>  4

Asp Val Gln Leu Gln Glu Ser Gly Pro Ser Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp 
            20                  25                  30          


Phe Ala Trp Asn Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp 
        35                  40                  45              


Met Gly Tyr Ile Ser Tyr Ser Gly Asn Thr Arg Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln Phe Phe 
65                  70                  75                  80  


Leu Gln Leu Asn Ser Val Thr Ile Glu Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Val Thr Ala Gly Arg Gly Phe Pro Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ala 
        115     


<210>  5
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LCDR1

<400>  5

His Ser Ser Gln Asp Ile Asn Ser Asn Ile Gly 
1               5                   10      


<210>  6
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LCDR2

<400>  6

His Gly Thr Asn Leu Asp Asp 
1               5           


<210>  7
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LCDR3

<400>  7

Val Gln Tyr Ala Gln Phe Pro Trp Thr 
1               5                   


<210>  8
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HCDR1

<400>  8

Gly Tyr Ser Ile Thr Ser Asp Phe Ala Trp Asn 
1               5                   10      


<210>  9
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HCDR2

<400>  9

Gly Tyr Ile Ser Tyr Ser Gly Asn Thr Arg Tyr Asn Pro Ser Leu Lys 
1               5                   10                  15      


<210>  10
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HCDR3

<400>  10

Val Thr Ala Gly Arg Gly Phe Pro Tyr Trp 
1               5                   10  


<210>  11
<211>  135
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8 hinge

<400>  11
accactaccc cagcaccgag gccacccacc ccggctccta ccatcgcctc ccagcctctg       60

tccctgcgtc cggaggcatg tagacccgca gctggtgggg ccgtgcatac ccggggtctt      120

gacttcgcct gcgat                                                       135


<210>  12
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8 transmembrane

<400>  12
atctacattt gggcccctct ggctggtact tgcggggtcc tgctgctttc actcgtgatc       60

actctttact gt                                                           72


<210>  13
<211>  126
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  41BB

<400>  13
aagcgcggtc ggaagaagct gctgtacatc tttaagcaac ccttcatgag gcctgtgcag       60

actactcaag aggaggacgg ctgttcatgc cggttcccag aggaggagga aggcggctgc      120

gaactg                                                                 126


<210>  14
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD3zeta

<400>  14
cgcgtgaaat tcagccgcag cgcagatgct ccagcctaca agcaggggca gaaccagctc       60

tacaacgaac tcaatcttgg tcggagagag gagtacgacg tgctggacaa gcggagagga      120

cgggacccag aaatgggcgg gaagccgcgc agaaagaatc cccaagaggg cctgtacaac      180

gagctccaaa aggataagat ggcagaagcc tatagcgaga ttggtatgaa aggggaacgc      240

agaagaggca aaggccacga cggactgtac cagggactca gcaccgccac caaggacacc      300

tatgacgctc ttcacatgca ggccctgccg cctcgg                                336


<210>  15
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8 SRP

<400>  15
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccg                                                                     63


<210>  16
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DAP12

<400>  16
atggggggac ttgaaccctg cagcaggttc ctgctcctgc ctctcctgct ggctgtaagt       60

ggtctccgtc ctgtccaggt ccaggcccag agcgattgca gttgctctac ggtgagcccg      120

ggcgtgctgg cagggatcgt gatgggagac ctggtgctga cagtgctcat tgccctggcc      180

gtgtacttcc tgggccggct ggtccctcgg gggcgagggg ctgcggaggc agcgacccgg      240

aaacagcgta tcactgagac cgagtcgcct tatcaggagc tccagggtca gaggtcggat      300

gtctacagcg acctcaacac acagaggccg tattacaaa                             339


<210>  17
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T2A

<400>  17
gtcgagggcg gcggagaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat       60

cccggcccta gg                                                           72


<210>  18
<211>  258
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Linker KIRS2

<400>  18
ggtggcggag gttctggagg tgggggttcc tcacccactg aaccaagctc caaaaccggt       60

aaccccagac acctgcatgt tctgattggg acctcagtgg tcaaaatccc tttcaccatc      120

ctcctcttct ttctccttca tcgctggtgc tccaacaaaa aaaatgctgc tgtaatggac      180

caagagcctg cagggaacag aacagtgaac agcgaggatt ctgatgaaca agaccatcag      240

gaggtgtcat acgcataa                                                    258


<210>  19
<211>  1464
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  806 BBZ CAR

<400>  19
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgggatccg atgtccagct gcaagagtct ggccctagcc tggtcaagcc tagccagagc      120

ctgagcctga catgtaccgt gaccggctac agcatcacca gcgacttcgc ctggaactgg      180

atcagacagt tccccggcaa caagctggaa tggatgggct acatcagcta cagcggcaac      240

acccggtaca accccagcct gaagtcccgg atctccatca ccagagacac cagcaagaac      300

cagttcttcc tgcagctgaa cagcgtgacc atcgaggaca ccgccaccta ctactgtgtg      360

acagccggca gaggcttccc ttattgggga cagggaaccc tggtcacagt gtctgctggt      420

ggcggaggat ctggcggagg cggatcttct ggcggtggct ctgatatcct gatgacacag      480

agccccagca gcatgtctgt gtccctgggc gataccgtgt ccatcacctg tcacagcagc      540

caggacatca acagcaacat cggctggctg cagcagaggc ctggcaagtc ttttaagggc      600

ctgatctacc acggcaccaa cctggatgat gaggtgccca gcagattttc cggctctgga      660

agcggagccg actactccct gacaatcagc agcctggaaa gcgaggactt cgccgattac      720

tactgcgtgc agtacgccca gtttccttgg acctttggag gcggcacaaa gctggaaatc      780

aagcgggcta gcaccactac cccagcaccg aggccaccca ccccggctcc taccatcgcc      840

tcccagcctc tgtccctgcg tccggaggca tgtagacccg cagctggtgg ggccgtgcat      900

acccggggtc ttgacttcgc ctgcgatatc tacatttggg cccctctggc tggtacttgc      960

ggggtcctgc tgctttcact cgtgatcact ctttactgta agcgcggtcg gaagaagctg     1020

ctgtacatct ttaagcaacc cttcatgagg cctgtgcaga ctactcaaga ggaggacggc     1080

tgttcatgcc ggttcccaga ggaggaggaa ggcggctgcg aactgcgcgt gaaattcagc     1140

cgcagcgcag atgctccagc ctacaagcag gggcagaacc agctctacaa cgaactcaat     1200

cttggtcgga gagaggagta cgacgtgctg gacaagcgga gaggacggga cccagaaatg     1260

ggcgggaagc cgcgcagaaa gaatccccaa gagggcctgt acaacgagct ccaaaaggat     1320

aagatggcag aagcctatag cgagattggt atgaaagggg aacgcagaag aggcaaaggc     1380

cacgacggac tgtaccaggg actcagcacc gccaccaagg acacctatga cgctcttcac     1440

atgcaggccc tgccgcctcg gtga                                            1464


<210>  20
<211>  487
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  806 BBZ CAR

<400>  20

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gly Ser Asp Val Gln Leu Gln Glu Ser Gly Pro 
            20                  25                  30          


Ser Leu Val Lys Pro Ser Gln Ser Leu Ser Leu Thr Cys Thr Val Thr 
        35                  40                  45              


Gly Tyr Ser Ile Thr Ser Asp Phe Ala Trp Asn Trp Ile Arg Gln Phe 
    50                  55                  60                  


Pro Gly Asn Lys Leu Glu Trp Met Gly Tyr Ile Ser Tyr Ser Gly Asn 
65                  70                  75                  80  


Thr Arg Tyr Asn Pro Ser Leu Lys Ser Arg Ile Ser Ile Thr Arg Asp 
                85                  90                  95      


Thr Ser Lys Asn Gln Phe Phe Leu Gln Leu Asn Ser Val Thr Ile Glu 
            100                 105                 110         


Asp Thr Ala Thr Tyr Tyr Cys Val Thr Ala Gly Arg Gly Phe Pro Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Ser Gly Gly Gly Ser Asp Ile Leu Met Thr Gln 
145                 150                 155                 160 


Ser Pro Ser Ser Met Ser Val Ser Leu Gly Asp Thr Val Ser Ile Thr 
                165                 170                 175     


Cys His Ser Ser Gln Asp Ile Asn Ser Asn Ile Gly Trp Leu Gln Gln 
            180                 185                 190         


Arg Pro Gly Lys Ser Phe Lys Gly Leu Ile Tyr His Gly Thr Asn Leu 
        195                 200                 205             


Asp Asp Glu Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Ala Asp 
    210                 215                 220                 


Tyr Ser Leu Thr Ile Ser Ser Leu Glu Ser Glu Asp Phe Ala Asp Tyr 
225                 230                 235                 240 


Tyr Cys Val Gln Tyr Ala Gln Phe Pro Trp Thr Phe Gly Gly Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Arg Ala Ser Thr Thr Thr Pro Ala Pro Arg Pro 
            260                 265                 270         


Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro 
        275                 280                 285             


Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu 
    290                 295                 300                 


Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys 
305                 310                 315                 320 


Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly 
                325                 330                 335     


Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
            340                 345                 350         


Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
        355                 360                 365             


Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp 
    370                 375                 380                 


Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
385                 390                 395                 400 


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
                405                 410                 415     


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
            420                 425                 430         


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
        435                 440                 445             


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
    450                 455                 460                 


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
465                 470                 475                 480 


Met Gln Ala Leu Pro Pro Arg 
                485         


<210>  21
<211>  1461
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  806 KIR CAR

<400>  21
atggggggac ttgaaccctg cagcaggttc ctgctcctgc ctctcctgct ggctgtaagt       60

ggtctccgtc ctgtccaggt ccaggcccag agcgattgca gttgctctac ggtgagcccg      120

ggcgtgctgg cagggatcgt gatgggagac ctggtgctga cagtgctcat tgccctggcc      180

gtgtacttcc tgggccggct ggtccctcgg gggcgagggg ctgcggaggc agcgacccgg      240

aaacagcgta tcactgagac cgagtcgcct tatcaggagc tccagggtca gaggtcggat      300

gtctacagcg acctcaacac acagaggccg tattacaaag tcgagggcgg cggagagggc      360

agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctag gatggcctta      420

ccagtgaccg ccttgctcct gccgctggcc ttgctgctcc acgccgccag gccgggatcc      480

gatgtccagc tgcaagagtc tggccctagc ctggtcaagc ctagccagag cctgagcctg      540

acatgtaccg tgaccggcta cagcatcacc agcgacttcg cctggaactg gatcagacag      600

ttccccggca acaagctgga atggatgggc tacatcagct acagcggcaa cacccggtac      660

aaccccagcc tgaagtcccg gatctccatc accagagaca ccagcaagaa ccagttcttc      720

ctgcagctga acagcgtgac catcgaggac accgccacct actactgtgt gacagccggc      780

agaggcttcc cttattgggg acagggaacc ctggtcacag tgtctgctgg tggcggagga      840

tctggcggag gcggatcttc tggcggtggc tctgatatcc tgatgacaca gagccccagc      900

agcatgtctg tgtccctggg cgataccgtg tccatcacct gtcacagcag ccaggacatc      960

aacagcaaca tcggctggct gcagcagagg cctggcaagt cttttaaggg cctgatctac     1020

cacggcacca acctggatga tgaggtgccc agcagatttt ccggctctgg aagcggagcc     1080

gactactccc tgacaatcag cagcctggaa agcgaggact tcgccgatta ctactgcgtg     1140

cagtacgccc agtttccttg gacctttgga ggcggcacaa agctggaaat caagcgggct     1200

agcggtggcg gaggttctgg aggtgggggt tcctcaccca ctgaaccaag ctccaaaacc     1260

ggtaacccca gacacctgca tgttctgatt gggacctcag tggtcaaaat ccctttcacc     1320

atcctcctct tctttctcct tcatcgctgg tgctccaaca aaaaaaatgc tgctgtaatg     1380

gaccaagagc ctgcagggaa cagaacagtg aacagcgagg attctgatga acaagaccat     1440

caggaggtgt catacgcata a                                               1461


<210>  22
<211>  486
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  806 KIR CAR

<400>  22

Met Gly Gly Leu Glu Pro Cys Ser Arg Phe Leu Leu Leu Pro Leu Leu 
1               5                   10                  15      


Leu Ala Val Ser Gly Leu Arg Pro Val Gln Val Gln Ala Gln Ser Asp 
            20                  25                  30          


Cys Ser Cys Ser Thr Val Ser Pro Gly Val Leu Ala Gly Ile Val Met 
        35                  40                  45              


Gly Asp Leu Val Leu Thr Val Leu Ile Ala Leu Ala Val Tyr Phe Leu 
    50                  55                  60                  


Gly Arg Leu Val Pro Arg Gly Arg Gly Ala Ala Glu Ala Ala Thr Arg 
65                  70                  75                  80  


Lys Gln Arg Ile Thr Glu Thr Glu Ser Pro Tyr Gln Glu Leu Gln Gly 
                85                  90                  95      


Gln Arg Ser Asp Val Tyr Ser Asp Leu Asn Thr Gln Arg Pro Tyr Tyr 
            100                 105                 110         


Lys Val Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly 
        115                 120                 125             


Asp Val Glu Glu Asn Pro Gly Pro Arg Met Ala Leu Pro Val Thr Ala 
    130                 135                 140                 


Leu Leu Leu Pro Leu Ala Leu Leu Leu His Ala Ala Arg Pro Gly Ser 
145                 150                 155                 160 


Asp Val Gln Leu Gln Glu Ser Gly Pro Ser Leu Val Lys Pro Ser Gln 
                165                 170                 175     


Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp 
            180                 185                 190         


Phe Ala Trp Asn Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp 
        195                 200                 205             


Met Gly Tyr Ile Ser Tyr Ser Gly Asn Thr Arg Tyr Asn Pro Ser Leu 
    210                 215                 220                 


Lys Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln Phe Phe 
225                 230                 235                 240 


Leu Gln Leu Asn Ser Val Thr Ile Glu Asp Thr Ala Thr Tyr Tyr Cys 
                245                 250                 255     


Val Thr Ala Gly Arg Gly Phe Pro Tyr Trp Gly Gln Gly Thr Leu Val 
            260                 265                 270         


Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Gly 
        275                 280                 285             


Gly Gly Ser Asp Ile Leu Met Thr Gln Ser Pro Ser Ser Met Ser Val 
    290                 295                 300                 


Ser Leu Gly Asp Thr Val Ser Ile Thr Cys His Ser Ser Gln Asp Ile 
305                 310                 315                 320 


Asn Ser Asn Ile Gly Trp Leu Gln Gln Arg Pro Gly Lys Ser Phe Lys 
                325                 330                 335     


Gly Leu Ile Tyr His Gly Thr Asn Leu Asp Asp Glu Val Pro Ser Arg 
            340                 345                 350         


Phe Ser Gly Ser Gly Ser Gly Ala Asp Tyr Ser Leu Thr Ile Ser Ser 
        355                 360                 365             


Leu Glu Ser Glu Asp Phe Ala Asp Tyr Tyr Cys Val Gln Tyr Ala Gln 
    370                 375                 380                 


Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala 
385                 390                 395                 400 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Pro Thr Glu Pro 
                405                 410                 415     


Ser Ser Lys Thr Gly Asn Pro Arg His Leu His Val Leu Ile Gly Thr 
            420                 425                 430         


Ser Val Val Lys Ile Pro Phe Thr Ile Leu Leu Phe Phe Leu Leu His 
        435                 440                 445             


Arg Trp Cys Ser Asn Lys Lys Asn Ala Ala Val Met Asp Gln Glu Pro 
    450                 455                 460                 


Ala Gly Asn Arg Thr Val Asn Ser Glu Asp Ser Asp Glu Gln Asp His 
465                 470                 475                 480 


Gln Glu Val Ser Tyr Ala 
                485     


<210>  23
<211>  348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Humanized 806 VH

<400>  23
caggttcagc tgcaagagtc tggccctggc ctggtcaagc ctagccaaac actgagcctg       60

acctgtaccg tgtccggcta cagcatcagc agcgacttcg cctggaactg gatcagacag      120

cctcctggca aaggactgga atggatgggc tacatcagct acagcggcaa caccagatac      180

cagcctagcc tgaagtcccg gatcaccatc agcagagaca ccagcaagaa ccagttcttc      240

ctgaagctga acagcgtgac agccgccgat accgccacct actattgtgt gacagctggc      300

agaggcttcc cctattgggg acagggaaca ctggtcaccg ttagctct                   348


<210>  24
<211>  324
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Humanized 806 VL

<400>  24
gatatccaga tgacacagag ccccagcagc atgtccgtgt ccgtgggaga cagagtgacc       60

atcacctgtc acagcagcca ggacatcaac agcaacatcg gctggctgca gcagaagccc      120

ggcaagtctt ttaagggcct gatctaccac ggcaccaacc tggatgatgg cgtgcccagc      180

agattttctg gcagcggctc tggcaccgac tacaccctga ccatatctag cctgcagcct      240

gaggacttcg ccacctatta ctgcgtgcag tacgcccagt ttccttggac ctttggaggc      300

ggcacaaagc tggaaatcaa gcgg                                             324


<210>  25
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Humanized 806 scFv

<400>  25
caggttcagc tgcaagagtc tggccctggc ctggtcaagc ctagccaaac actgagcctg       60

acctgtaccg tgtccggcta cagcatcagc agcgacttcg cctggaactg gatcagacag      120

cctcctggca aaggactgga atggatgggc tacatcagct acagcggcaa caccagatac      180

cagcctagcc tgaagtcccg gatcaccatc agcagagaca ccagcaagaa ccagttcttc      240

ctgaagctga acagcgtgac agccgccgat accgccacct actattgtgt gacagctggc      300

agaggcttcc cctattgggg acagggaaca ctggtcaccg ttagctctga tatccagatg      360

acacagagcc ccagcagcat gtccgtgtcc gtgggagaca gagtgaccat cacctgtcac      420

agcagccagg acatcaacag caacatcggc tggctgcagc agaagcccgg caagtctttt      480

aagggcctga tctaccacgg caccaacctg gatgatggcg tgcccagcag attttctggc      540

agcggctctg gcaccgacta caccctgacc atatctagcc tgcagcctga ggacttcgcc      600

acctattact gcgtgcagta cgcccagttt ccttggacct ttggaggcgg cacaaagctg      660

gaaatcaagc gg                                                          672


<210>  26
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 806 VH

<400>  26

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Tyr Ser Ile Ser Ser Asp 
            20                  25                  30          


Phe Ala Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Met Gly Tyr Ile Ser Tyr Ser Gly Asn Thr Arg Tyr Gln Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Ile Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Phe Phe 
65                  70                  75                  80  


Leu Lys Leu Asn Ser Val Thr Ala Ala Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Val Thr Ala Gly Arg Gly Phe Pro Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  27
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 806 VL

<400>  27

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Met Ser Val Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys His Ser Ser Gln Asp Ile Asn Ser Asn 
            20                  25                  30          


Ile Gly Trp Leu Gln Gln Lys Pro Gly Lys Ser Phe Lys Gly Leu Ile 
        35                  40                  45              


Tyr His Gly Thr Asn Leu Asp Asp Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Val Gln Tyr Ala Gln Phe Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105             


<210>  28
<211>  224
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 806 scFv

<400>  28

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Tyr Ser Ile Ser Ser Asp 
            20                  25                  30          


Phe Ala Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Met Gly Tyr Ile Ser Tyr Ser Gly Asn Thr Arg Tyr Gln Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Ile Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Phe Phe 
65                  70                  75                  80  


Leu Lys Leu Asn Ser Val Thr Ala Ala Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Val Thr Ala Gly Arg Gly Phe Pro Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Met Ser 
        115                 120                 125             


Val Ser Val Gly Asp Arg Val Thr Ile Thr Cys His Ser Ser Gln Asp 
    130                 135                 140                 


Ile Asn Ser Asn Ile Gly Trp Leu Gln Gln Lys Pro Gly Lys Ser Phe 
145                 150                 155                 160 


Lys Gly Leu Ile Tyr His Gly Thr Asn Leu Asp Asp Gly Val Pro Ser 
                165                 170                 175     


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser 
            180                 185                 190         


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Val Gln Tyr Ala 
        195                 200                 205             


Gln Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
    210                 215                 220                 


<210>  29
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affinity maturized 806 VH

<400>  29

Glu Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Tyr Ser Ile Ser Arg Asp 
            20                  25                  30          


Phe Ala Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Met Gly Tyr Ile Ser Tyr Asn Gly Asn Thr Arg Tyr Gln Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Ile Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Phe Phe 
65                  70                  75                  80  


Leu Lys Leu Asn Ser Val Thr Ala Ala Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Val Thr Ala Ser Arg Gly Phe Pro Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  30
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affinity maturized 806 VL

<400>  30

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Met Ser Val Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys His Ser Ser Gln Asp Ile Asn Ser Asn 
            20                  25                  30          


Ile Gly Trp Leu Gln Gln Lys Pro Gly Lys Ser Phe Lys Gly Leu Ile 
        35                  40                  45              


Tyr His Gly Thr Asn Leu Asp Asp Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Val Gln Tyr Ala Gln Phe Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  31
<211>  717
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  806 scFv

<400>  31

Gly Ala Thr Ala Thr Thr Cys Thr Gly Ala Thr Gly Ala Cys Thr Cys 
1               5                   10                  15      


Ala Ala Thr Cys Thr Cys Cys Gly Thr Cys Thr Thr Cys Thr Ala Thr 
            20                  25                  30          


Gly Ala Gly Cys Gly Thr Gly Ala Gly Cys Thr Thr Gly Gly Gly Thr 
        35                  40                  45              


Gly Ala Cys Ala Cys Cys Gly Thr Cys Ala Gly Cys Ala Thr Cys Ala 
    50                  55                  60                  


Cys Cys Thr Gly Thr Cys Ala Thr Thr Cys Cys Ala Gly Cys Cys Ala 
65                  70                  75                  80  


Gly Gly Ala Thr Ala Thr Ala Ala Ala Cys Thr Cys Ala Ala Ala Thr 
                85                  90                  95      


Ala Thr Cys Gly Gly Cys Thr Gly Gly Cys Thr Cys Cys Ala Gly Cys 
            100                 105                 110         


Ala Ala Cys Gly Cys Cys Cys Ala Gly Gly Cys Ala Ala Gly Thr Cys 
        115                 120                 125             


Ala Thr Thr Cys Ala Ala Gly Gly Gly Gly Cys Thr Thr Ala Thr Thr 
    130                 135                 140                 


Thr Ala Thr Cys Ala Thr Gly Gly Cys Ala Cys Cys Ala Ala Thr Cys 
145                 150                 155                 160 


Thr Thr Gly Ala Cys Gly Ala Thr Gly Ala Ala Gly Thr Cys Cys Cys 
                165                 170                 175     


Ala Thr Cys Ala Cys Gly Cys Thr Thr Cys Ala Gly Cys Gly Gly Ala 
            180                 185                 190         


Thr Cys Ala Gly Gly Cys Thr Cys Ala Gly Gly Thr Gly Cys Gly Gly 
        195                 200                 205             


Ala Cys Thr Ala Thr Thr Cys Cys Thr Thr Gly Ala Cys Thr Ala Thr 
    210                 215                 220                 


Ala Ala Gly Thr Thr Cys Cys Cys Thr Cys Gly Ala Ala Thr Cys Thr 
225                 230                 235                 240 


Gly Ala Gly Gly Ala Thr Thr Thr Cys Gly Cys Cys Gly Ala Cys Thr 
                245                 250                 255     


Ala Thr Thr Ala Thr Thr Gly Cys Gly Thr Ala Cys Ala Ala Thr Ala 
            260                 265                 270         


Cys Gly Cys Cys Cys Ala Gly Thr Thr Thr Cys Cys Cys Thr Gly Gly 
        275                 280                 285             


Ala Cys Cys Thr Thr Cys Gly Gly Ala Gly Gly Cys Gly Gly Cys Ala 
    290                 295                 300                 


Cys Cys Ala Ala Ala Thr Thr Gly Gly Ala Gly Ala Thr Ala Ala Ala 
305                 310                 315                 320 


Ala Ala Gly Gly Gly Gly Thr Gly Gly Ala Gly Gly Ala Gly Gly Ala 
                325                 330                 335     


Thr Cys Ala Gly Gly Cys Gly Gly Gly Gly Gly Thr Gly Gly Ala Ala 
            340                 345                 350         


Gly Cys Gly Gly Cys Gly Gly Ala Gly Gly Ala Gly Gly Cys Ala Gly 
        355                 360                 365             


Cys Gly Ala Cys Gly Thr Ala Cys Ala Ala Cys Thr Gly Cys Ala Ala 
    370                 375                 380                 


Gly Ala Ala Thr Cys Cys Gly Gly Gly Cys Cys Gly Ala Gly Thr Thr 
385                 390                 395                 400 


Thr Gly Gly Thr Cys Ala Ala Gly Cys Cys Cys Thr Cys Thr Cys Ala 
                405                 410                 415     


Ala Thr Cys Thr Cys Thr Thr Thr Cys Thr Cys Thr Cys Ala Cys Thr 
            420                 425                 430         


Thr Gly Cys Ala Cys Gly Gly Thr Cys Ala Cys Cys Gly Gly Ala Thr 
        435                 440                 445             


Ala Cys Thr Cys Cys Ala Thr Ala Ala Cys Cys Ala Gly Cys Gly Ala 
    450                 455                 460                 


Thr Thr Thr Thr Gly Cys Gly Thr Gly Gly Ala Ala Thr Thr Gly Gly 
465                 470                 475                 480 


Ala Thr Thr Cys Gly Ala Cys Ala Ala Thr Thr Thr Cys Cys Ala Gly 
                485                 490                 495     


Gly Gly Ala Ala Thr Ala Ala Ala Thr Thr Gly Gly Ala Ala Thr Gly 
            500                 505                 510         


Gly Ala Thr Gly Gly Gly Ala Thr Ala Thr Ala Thr Cys Ala Gly Thr 
        515                 520                 525             


Thr Ala Thr Thr Cys Thr Gly Gly Thr Ala Ala Thr Ala Cys Cys Ala 
    530                 535                 540                 


Gly Ala Thr Ala Cys Ala Ala Cys Cys Cys Gly Thr Cys Ala Thr Thr 
545                 550                 555                 560 


Gly Ala Ala Ala Ala Gly Thr Cys Gly Cys Ala Thr Cys Thr Cys Thr 
                565                 570                 575     


Ala Thr Ala Ala Cys Ala Cys Gly Ala Gly Ala Cys Ala Cys Thr Thr 
            580                 585                 590         


Cys Ala Ala Ala Gly Ala Ala Thr Cys Ala Gly Thr Thr Cys Thr Thr 
        595                 600                 605             


Cys Cys Thr Thr Cys Ala Gly Cys Thr Cys Ala Ala Thr Thr Cys Thr 
    610                 615                 620                 


Gly Thr Ala Ala Cys Cys Ala Thr Cys Gly Ala Ala Gly Ala Thr Ala 
625                 630                 635                 640 


Cys Thr Gly Cys Thr Ala Cys Thr Thr Ala Thr Thr Ala Cys Thr Gly 
                645                 650                 655     


Thr Gly Thr Ala Ala Cys Gly Gly Cys Gly Gly Gly Thr Cys Gly Ala 
            660                 665                 670         


Gly Gly Ala Thr Thr Cys Cys Cys Cys Thr Ala Cys Thr Gly Gly Gly 
        675                 680                 685             


Gly Cys Cys Ala Gly Gly Gly Thr Ala Cys Ala Cys Thr Gly Gly Thr 
    690                 695                 700                 


Thr Ala Cys Thr Gly Thr Thr Thr Cys Cys Gly Cys Cys 
705                 710                 715         


<210>  32
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  806 scFv

<400>  32

Asp Ile Leu Met Thr Gln Ser Pro Ser Ser Met Ser Val Ser Leu Gly 
1               5                   10                  15      


Asp Thr Val Ser Ile Thr Cys His Ser Ser Gln Asp Ile Asn Ser Asn 
            20                  25                  30          


Ile Gly Trp Leu Gln Gln Arg Pro Gly Lys Ser Phe Lys Gly Leu Ile 
        35                  40                  45              


Tyr His Gly Thr Asn Leu Asp Asp Glu Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Ala Asp Tyr Ser Leu Thr Ile Ser Ser Leu Glu Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Asp Tyr Tyr Cys Val Gln Tyr Ala Gln Phe Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly 
            100                 105                 110         


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Gln Leu Gln 
        115                 120                 125             


Glu Ser Gly Pro Ser Leu Val Lys Pro Ser Gln Ser Leu Ser Leu Thr 
    130                 135                 140                 


Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp Phe Ala Trp Asn Trp 
145                 150                 155                 160 


Ile Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp Met Gly Tyr Ile Ser 
                165                 170                 175     


Tyr Ser Gly Asn Thr Arg Tyr Asn Pro Ser Leu Lys Ser Arg Ile Ser 
            180                 185                 190         


Ile Thr Arg Asp Thr Ser Lys Asn Gln Phe Phe Leu Gln Leu Asn Ser 
        195                 200                 205             


Val Thr Ile Glu Asp Thr Ala Thr Tyr Tyr Cys Val Thr Ala Gly Arg 
    210                 215                 220                 


Gly Phe Pro Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala 
225                 230                 235                 


<210>  33
<211>  324
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  806 VL

<400>  33
gatatcctga tgacacagag ccccagcagc atgtctgtgt ccctgggcga taccgtgtcc       60

atcacctgtc acagcagcca ggacatcaac agcaacatcg gctggctgca gcagaggcct      120

ggcaagtctt ttaagggcct gatctaccac ggcaccaacc tggatgatga ggtgcccagc      180

agattttccg gctctggaag cggagccgac tactccctga caatcagcag cctggaaagc      240

gaggacttcg ccgattacta ctgcgtgcag tacgcccagt ttccttggac ctttggaggc      300

ggcacaaagc tggaaatcaa gcgg                                             324


<210>  34
<211>  348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  806 VH

<400>  34
gatgtccagc tgcaagagtc tggccctagc ctggtcaagc ctagccagag cctgagcctg       60

acatgtaccg tgaccggcta cagcatcacc agcgacttcg cctggaactg gatcagacag      120

ttccccggca acaagctgga atggatgggc tacatcagct acagcggcaa cacccggtac      180

aaccccagcc tgaagtcccg gatctccatc accagagaca ccagcaagaa ccagttcttc      240

ctgcagctga acagcgtgac catcgaggac accgccacct actactgtgt gacagccggc      300

agaggcttcc cttattgggg acagggaacc ctggtcacag tgtctgct                   348


<210>  35
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LCDR2

<400>  35

His Gly Thr Asn Leu Asp Asp 
1               5           


<210>  36
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker


<220>
<221>  REPEAT
<222>  (1)..(5)
<223>  repeat n times, where n represents an integer of at least 1

<400>  36

Gly Ser Gly Gly Ser 
1               5   


<210>  37
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker


<220>
<221>  REPEAT
<222>  (1)..(4)
<223>  repeat n times, where n represents an integer of at least 1

<400>  37

Gly Gly Gly Ser 
1               


<210>  38
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker


<220>
<221>  REPEAT
<222>  (1)..(5)
<223>  repeat n times, where n represents an integer of at least 1

<400>  38

Gly Gly Gly Gly Ser 
1               5   


<210>  39
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  39

Gly Gly Ser Gly 
1               


<210>  40
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  40

Gly Gly Ser Gly Gly 
1               5   


<210>  41
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  41

Gly Ser Gly Ser Gly 
1               5   


<210>  42
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  42

Gly Ser Gly Gly Gly 
1               5   


<210>  43
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  43

Gly Gly Gly Ser Gly 
1               5   


<210>  44
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  44

Gly Ser Ser Ser Gly 
1               5   


<210>  45
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  45

Gly Gly Gly Gly Ser 
1               5   


<210>  46
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  46

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  47
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  47
ggtggcggtg gctcgggcgg tggtgggtcg ggtggcggcg gatct                       45


<210>  48
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  48

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Gly Gly Gly Ser 
1               5                   10                  15  


<210>  49
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  49
ggtggcggag gatctggcgg aggcggatct tctggcggtg gctct                       45


<210>  50
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  50

Asp Lys Thr His Thr 
1               5   


<210>  51
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  51

Cys Pro Pro Cys 
1               


<210>  52
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  52

Cys Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg 
1               5                   10                  15  


<210>  53
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  53

Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr 
1               5                   10          


<210>  54
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  54

Lys Ser Cys Asp Lys Thr His Thr Cys Pro 
1               5                   10  


<210>  55
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  55

Lys Cys Cys Val Asp Cys Pro 
1               5           


<210>  56
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  56

Lys Tyr Gly Pro Pro Cys Pro 
1               5           


<210>  57
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  57

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  58
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  58

Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro 
1               5                   10          


<210>  59
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  59

Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Cys Pro Arg Cys 
1               5                   10                  15      


Pro 
    


<210>  60
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  60

Ser Pro Asn Met Val Pro His Ala His His Ala Gln 
1               5                   10          


<210>  61
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  61

Glu Pro Lys Ser Cys Asp Lys Thr Tyr Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  62
<211>  348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Affinity maturated humanized 806 VH

<400>  62
gaggttcagc tgcaagagtc tggccctggc ctggtcaagc ctagccaaac actgagcctg       60

acctgtaccg tgtccggcta cagcatcagc agagacttcg cctggaactg gatcagacag      120

cctcctggca aaggactgga atggatgggc tacatcagct acaacggcaa caccagatac      180

cagcctagcc tgaagtcccg gatcaccatc tccagagaca ccagcaagaa ccagttcttc      240

ctgaagctga acagcgtgac agccgccgat accgccacct actattgtgt gacagccagc      300

agaggcttcc cctattgggg acagggaacc ctggtcacag ttagctct                   348


<210>  63
<211>  321
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affinity maturated humanized 806 VL

<400>  63

Gly Ala Thr Ala Thr Cys Cys Ala Gly Ala Thr Gly Ala Cys Ala Cys 
1               5                   10                  15      


Ala Gly Ala Gly Cys Cys Cys Cys Ala Gly Cys Ala Gly Cys Ala Thr 
            20                  25                  30          


Gly Thr Cys Cys Gly Thr Gly Thr Cys Cys Gly Thr Gly Gly Gly Ala 
        35                  40                  45              


Gly Ala Cys Ala Gly Ala Gly Thr Gly Ala Cys Cys Ala Thr Cys Ala 
    50                  55                  60                  


Cys Cys Thr Gly Thr Cys Ala Cys Ala Gly Cys Ala Gly Cys Cys Ala 
65                  70                  75                  80  


Gly Gly Ala Cys Ala Thr Cys Ala Ala Cys Ala Gly Cys Ala Ala Cys 
                85                  90                  95      


Ala Thr Cys Gly Gly Cys Thr Gly Gly Cys Thr Gly Cys Ala Gly Cys 
            100                 105                 110         


Ala Gly Ala Ala Gly Cys Cys Cys Gly Gly Cys Ala Ala Gly Thr Cys 
        115                 120                 125             


Thr Thr Thr Thr Ala Ala Gly Gly Gly Cys Cys Thr Gly Ala Thr Cys 
    130                 135                 140                 


Thr Ala Cys Cys Ala Cys Gly Gly Cys Ala Cys Cys Ala Ala Cys Cys 
145                 150                 155                 160 


Thr Gly Gly Ala Thr Gly Ala Thr Gly Gly Cys Gly Thr Gly Cys Cys 
                165                 170                 175     


Cys Ala Gly Cys Ala Gly Ala Thr Thr Thr Thr Cys Thr Gly Gly Cys 
            180                 185                 190         


Ala Gly Cys Gly Gly Cys Thr Cys Thr Gly Gly Cys Ala Cys Cys Gly 
        195                 200                 205             


Ala Cys Thr Ala Cys Ala Cys Cys Cys Thr Gly Ala Cys Cys Ala Thr 
    210                 215                 220                 


Ala Thr Cys Thr Ala Gly Cys Cys Thr Gly Cys Ala Gly Cys Cys Thr 
225                 230                 235                 240 


Gly Ala Gly Gly Ala Cys Thr Thr Cys Gly Cys Cys Ala Cys Cys Thr 
                245                 250                 255     


Ala Thr Thr Ala Cys Thr Gly Cys Gly Thr Gly Cys Ala Gly Thr Ala 
            260                 265                 270         


Cys Gly Cys Cys Cys Ala Gly Thr Thr Thr Cys Cys Thr Thr Gly Gly 
        275                 280                 285             


Ala Cys Cys Thr Thr Thr Gly Gly Ala Gly Gly Cys Gly Gly Cys Ala 
    290                 295                 300                 


Cys Ala Ala Ala Gly Cys Thr Gly Gly Ala Ala Ala Thr Cys Ala Ala 
305                 310                 315                 320 


Gly 
    


<210>  64
<211>  1449
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  806 CAR

<400>  64
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccggatgtcc agctgcaaga gtctggccct agcctggtca agcctagcca gagcctgagc      120

ctgacatgta ccgtgaccgg ctacagcatc accagcgact tcgcctggaa ctggatcaga      180

cagttccccg gcaacaagct ggaatggatg ggctacatca gctacagcgg caacacccgg      240

tacaacccca gcctgaagtc ccggatctcc atcaccagag acaccagcaa gaaccagttc      300

ttcctgcagc tgaacagcgt gaccatcgag gacaccgcca cctactactg tgtgacagcc      360

ggcagaggct tcccttattg gggacaggga accctggtca cagtgtctgc tggtggcgga      420

ggatctggcg gaggcggatc ttctggcggt ggctctgata tcctgatgac acagagcccc      480

agcagcatgt ctgtgtccct gggcgatacc gtgtccatca cctgtcacag cagccaggac      540

atcaacagca acatcggctg gctgcagcag aggcctggca agtcttttaa gggcctgatc      600

taccacggca ccaacctgga tgatgaggtg cccagcagat tttccggctc tggaagcgga      660

gccgactact ccctgacaat cagcagcctg gaaagcgagg acttcgccga ttactactgc      720

gtgcagtacg cccagtttcc ttggaccttt ggaggcggca caaagctgga aatcaagcgg      780

accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg      840

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      900

gacttcgcct gtgatatcta catctgggcc cctctggccg gcacctgtgg cgtgctgctg      960

ctgtccctgg tcatcaccct gtactgcaag cggggcagaa agaagctgct gtacatcttc     1020

aagcagccct tcatgcggcc tgtgcagacc acacaggaag aggacggctg tagctgtaga     1080

ttccccgagg aagaggaagg cggctgcgag ctgagagtga agttcagcag aagcgccgac     1140

gcccctgcct atcagcaggg ccagaaccag ctgtacaacg agctgaacct gggcagacgg     1200

gaggaatacg acgtgctgga caagagaaga ggccgggacc ctgagatggg cggcaagccc     1260

agacggaaga acccccagga aggcctgtat aacgaactgc agaaagacaa gatggccgag     1320

gcctacagcg agatcggcat gaagggcgag cggagaagag gcaagggcca tgacggcctg     1380

taccagggcc tgagcaccgc caccaaggac acctacgacg ccctgcacat gcaggccctg     1440

cctccaaga                                                             1449


<210>  65
<211>  483
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  806 CAR

<400>  65

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Val Gln Leu Gln Glu Ser Gly Pro Ser Leu 
            20                  25                  30          


Val Lys Pro Ser Gln Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr 
        35                  40                  45              


Ser Ile Thr Ser Asp Phe Ala Trp Asn Trp Ile Arg Gln Phe Pro Gly 
    50                  55                  60                  


Asn Lys Leu Glu Trp Met Gly Tyr Ile Ser Tyr Ser Gly Asn Thr Arg 
65                  70                  75                  80  


Tyr Asn Pro Ser Leu Lys Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser 
                85                  90                  95      


Lys Asn Gln Phe Phe Leu Gln Leu Asn Ser Val Thr Ile Glu Asp Thr 
            100                 105                 110         


Ala Thr Tyr Tyr Cys Val Thr Ala Gly Arg Gly Phe Pro Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Ser Gly Gly Gly Ser Asp Ile Leu Met Thr Gln Ser Pro 
145                 150                 155                 160 


Ser Ser Met Ser Val Ser Leu Gly Asp Thr Val Ser Ile Thr Cys His 
                165                 170                 175     


Ser Ser Gln Asp Ile Asn Ser Asn Ile Gly Trp Leu Gln Gln Arg Pro 
            180                 185                 190         


Gly Lys Ser Phe Lys Gly Leu Ile Tyr His Gly Thr Asn Leu Asp Asp 
        195                 200                 205             


Glu Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Ala Asp Tyr Ser 
    210                 215                 220                 


Leu Thr Ile Ser Ser Leu Glu Ser Glu Asp Phe Ala Asp Tyr Tyr Cys 
225                 230                 235                 240 


Val Gln Tyr Ala Gln Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu 
                245                 250                 255     


Glu Ile Lys Arg Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala 
            260                 265                 270         


Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg 
        275                 280                 285             


Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
    290                 295                 300                 


Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu 
305                 310                 315                 320 


Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu 
                325                 330                 335     


Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln 
            340                 345                 350         


Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly 
        355                 360                 365             


Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
    370                 375                 380                 


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
385                 390                 395                 400 


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
                405                 410                 415     


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
            420                 425                 430         


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
        435                 440                 445             


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
    450                 455                 460                 


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
465                 470                 475                 480 


Pro Pro Arg 
            


<210>  66
<211>  1449
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Humanized 806 CAR

<400>  66
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgcaggttc agctgcaaga gtctggccct ggcctggtca agcctagcca aacactgagc      120

ctgacctgta ccgtgtccgg ctacagcatc agcagcgact tcgcctggaa ctggatcaga      180

cagcctcctg gcaaaggact ggaatggatg ggctacatca gctacagcgg caacaccaga      240

taccagccta gcctgaagtc ccggatcacc atcagcagag acaccagcaa gaaccagttc      300

ttcctgaagc tgaacagcgt gacagccgcc gataccgcca cctactattg tgtgacagct      360

ggcagaggct tcccctattg gggacaggga acactggtca ccgttagctc tggtggcgga      420

ggatctggcg gaggcggatc ttctggcggt ggctctgata tccagatgac acagagcccc      480

agcagcatgt ccgtgtccgt gggagacaga gtgaccatca cctgtcacag cagccaggac      540

atcaacagca acatcggctg gctgcagcag aagcccggca agtcttttaa gggcctgatc      600

taccacggca ccaacctgga tgatggcgtg cccagcagat tttctggcag cggctctggc      660

accgactaca ccctgaccat atctagcctg cagcctgagg acttcgccac ctattactgc      720

gtgcagtacg cccagtttcc ttggaccttt ggaggcggca caaagctgga aatcaagcgg      780

accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg      840

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      900

gacttcgcct gtgatatcta catctgggcc cctctggccg gcacctgtgg cgtgctgctg      960

ctgtccctgg tcatcaccct gtactgcaag cggggcagaa agaagctgct gtacatcttc     1020

aagcagccct tcatgcggcc tgtgcagacc acacaggaag aggacggctg tagctgtaga     1080

ttccccgagg aagaggaagg cggctgcgag ctgagagtga agttcagcag aagcgccgac     1140

gcccctgcct atcagcaggg ccagaaccag ctgtacaacg agctgaacct gggcagacgg     1200

gaggaatacg acgtgctgga caagagaaga ggccgggacc ctgagatggg cggcaagccc     1260

agacggaaga acccccagga aggcctgtat aacgaactgc agaaagacaa gatggccgag     1320

gcctacagcg agatcggcat gaagggcgag cggagaagag gcaagggcca tgacggcctg     1380

taccagggcc tgagcaccgc caccaaggac acctacgacg ccctgcacat gcaggccctg     1440

cctccaaga                                                             1449


<210>  67
<211>  483
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 806 CAR

<400>  67

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu 
            20                  25                  30          


Val Lys Pro Ser Gln Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Tyr 
        35                  40                  45              


Ser Ile Ser Ser Asp Phe Ala Trp Asn Trp Ile Arg Gln Pro Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Met Gly Tyr Ile Ser Tyr Ser Gly Asn Thr Arg 
65                  70                  75                  80  


Tyr Gln Pro Ser Leu Lys Ser Arg Ile Thr Ile Ser Arg Asp Thr Ser 
                85                  90                  95      


Lys Asn Gln Phe Phe Leu Lys Leu Asn Ser Val Thr Ala Ala Asp Thr 
            100                 105                 110         


Ala Thr Tyr Tyr Cys Val Thr Ala Gly Arg Gly Phe Pro Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Ser Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro 
145                 150                 155                 160 


Ser Ser Met Ser Val Ser Val Gly Asp Arg Val Thr Ile Thr Cys His 
                165                 170                 175     


Ser Ser Gln Asp Ile Asn Ser Asn Ile Gly Trp Leu Gln Gln Lys Pro 
            180                 185                 190         


Gly Lys Ser Phe Lys Gly Leu Ile Tyr His Gly Thr Asn Leu Asp Asp 
        195                 200                 205             


Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr 
    210                 215                 220                 


Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys 
225                 230                 235                 240 


Val Gln Tyr Ala Gln Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu 
                245                 250                 255     


Glu Ile Lys Arg Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala 
            260                 265                 270         


Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg 
        275                 280                 285             


Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
    290                 295                 300                 


Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu 
305                 310                 315                 320 


Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu 
                325                 330                 335     


Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln 
            340                 345                 350         


Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly 
        355                 360                 365             


Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
    370                 375                 380                 


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
385                 390                 395                 400 


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
                405                 410                 415     


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
            420                 425                 430         


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
        435                 440                 445             


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
    450                 455                 460                 


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
465                 470                 475                 480 


Pro Pro Arg 
            


<210>  68
<211>  1446
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Affinity maturated humanized 806 CAR

<400>  68
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccggaggttc agctgcaaga gtctggccct ggcctggtca agcctagcca aacactgagc      120

ctgacctgta ccgtgtccgg ctacagcatc agcagagact tcgcctggaa ctggatcaga      180

cagcctcctg gcaaaggact ggaatggatg ggctacatca gctacaacgg caacaccaga      240

taccagccta gcctgaagtc ccggatcacc atctccagag acaccagcaa gaaccagttc      300

ttcctgaagc tgaacagcgt gacagccgcc gataccgcca cctactattg tgtgacagcc      360

agcagaggct tcccctattg gggacaggga accctggtca cagttagctc tggtggcgga      420

ggatctggcg gaggcggatc ttctggcggt ggctctgata tccagatgac acagagcccc      480

agcagcatgt ccgtgtccgt gggagacaga gtgaccatca cctgtcacag cagccaggac      540

atcaacagca acatcggctg gctgcagcag aagcccggca agtcttttaa gggcctgatc      600

taccacggca ccaacctgga tgatggcgtg cccagcagat tttctggcag cggctctggc      660

accgactaca ccctgaccat atctagcctg cagcctgagg acttcgccac ctattactgc      720

gtgcagtacg cccagtttcc ttggaccttt ggaggcggca caaagctgga aatcaagacc      780

acgacgccag cgccgcgacc accaacaccg gcgcccacca tcgcgtcgca gcccctgtcc      840

ctgcgcccag aggcgtgccg gccagcggcg gggggcgcag tgcacacgag ggggctggac      900

ttcgcctgtg atatctacat ctgggcccct ctggccggca cctgtggcgt gctgctgctg      960

tccctggtca tcaccctgta ctgcaagcgg ggcagaaaga agctgctgta catcttcaag     1020

cagcccttca tgcggcctgt gcagaccaca caggaagagg acggctgtag ctgtagattc     1080

cccgaggaag aggaaggcgg ctgcgagctg agagtgaagt tcagcagaag cgccgacgcc     1140

cctgcctatc agcagggcca gaaccagctg tacaacgagc tgaacctggg cagacgggag     1200

gaatacgacg tgctggacaa gagaagaggc cgggaccctg agatgggcgg caagcccaga     1260

cggaagaacc cccaggaagg cctgtataac gaactgcaga aagacaagat ggccgaggcc     1320

tacagcgaga tcggcatgaa gggcgagcgg agaagaggca agggccatga cggcctgtac     1380

cagggcctga gcaccgccac caaggacacc tacgacgccc tgcacatgca ggccctgcct     1440

ccaaga                                                                1446


<210>  69
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affinity maturated humanized 806 CAR

<400>  69

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Gln Glu Ser Gly Pro Gly Leu 
            20                  25                  30          


Val Lys Pro Ser Gln Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Tyr 
        35                  40                  45              


Ser Ile Ser Arg Asp Phe Ala Trp Asn Trp Ile Arg Gln Pro Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Met Gly Tyr Ile Ser Tyr Asn Gly Asn Thr Arg 
65                  70                  75                  80  


Tyr Gln Pro Ser Leu Lys Ser Arg Ile Thr Ile Ser Arg Asp Thr Ser 
                85                  90                  95      


Lys Asn Gln Phe Phe Leu Lys Leu Asn Ser Val Thr Ala Ala Asp Thr 
            100                 105                 110         


Ala Thr Tyr Tyr Cys Val Thr Ala Ser Arg Gly Phe Pro Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Ser Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro 
145                 150                 155                 160 


Ser Ser Met Ser Val Ser Val Gly Asp Arg Val Thr Ile Thr Cys His 
                165                 170                 175     


Ser Ser Gln Asp Ile Asn Ser Asn Ile Gly Trp Leu Gln Gln Lys Pro 
            180                 185                 190         


Gly Lys Ser Phe Lys Gly Leu Ile Tyr His Gly Thr Asn Leu Asp Asp 
        195                 200                 205             


Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr 
    210                 215                 220                 


Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys 
225                 230                 235                 240 


Val Gln Tyr Ala Gln Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu 
                245                 250                 255     


Glu Ile Lys Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
            260                 265                 270         


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
        275                 280                 285             


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
    290                 295                 300                 


Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
305                 310                 315                 320 


Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu 
                325                 330                 335     


Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu 
            340                 345                 350         


Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys 
        355                 360                 365             


Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
    370                 375                 380                 


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
385                 390                 395                 400 


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
                405                 410                 415     


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
            420                 425                 430         


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
        435                 440                 445             


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
    450                 455                 460                 


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
465                 470                 475                 480 


Pro Arg 
        


<210>  70
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SRP

<400>  70

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  71
<211>  135
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8 hinge

<400>  71
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg       60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      120

gacttcgcct gtgat                                                       135


<210>  72
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 hinge

<400>  72

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  73
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8 transmembrane

<400>  73
atctacatct gggcccctct ggccggcacc tgtggcgtgc tgctgctgtc cctggtcatc       60

accctgtact gc                                                           72


<210>  74
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 transmembrane

<400>  74

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  75
<211>  126
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  41BB

<400>  75
aagcggggca gaaagaagct gctgtacatc ttcaagcagc ccttcatgcg gcctgtgcag       60

accacacagg aagaggacgg ctgtagctgt agattccccg aggaagagga aggcggctgc      120

gagctg                                                                 126


<210>  76
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  41BB

<400>  76

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  77
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD3zeta

<400>  77
agagtgaagt tcagcagaag cgccgacgcc cctgcctatc agcagggcca gaaccagctg       60

tacaacgagc tgaacctggg cagacgggag gaatacgacg tgctggacaa gagaagaggc      120

cgggaccctg agatgggcgg caagcccaga cggaagaacc cccaggaagg cctgtataac      180

gaactgcaga aagacaagat ggccgaggcc tacagcgaga tcggcatgaa gggcgagcgg      240

agaagaggca agggccatga cggcctgtac cagggcctga gcaccgccac caaggacacc      300

tacgacgccc tgcacatgca ggccctgcct ccaaga                                336


<210>  78
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3zeta

<400>  78

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  79
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Humanized 806 scFv

<400>  79
caggttcagc tgcaagagtc tggccctggc ctggtcaagc ctagccaaac actgagcctg       60

acctgtaccg tgtccggcta cagcatcagc agcgacttcg cctggaactg gatcagacag      120

cctcctggca aaggactgga atggatgggc tacatcagct acagcggcaa caccagatac      180

cagcctagcc tgaagtcccg gatcaccatc agcagagaca ccagcaagaa ccagttcttc      240

ctgaagctga acagcgtgac agccgccgat accgccacct actattgtgt gacagctggc      300

agaggcttcc cctattgggg acagggaaca ctggtcaccg ttagctctgg tggcggagga      360

tctggcggag gcggatcttc tggcggtggc tctgatatcc agatgacaca gagccccagc      420

agcatgtccg tgtccgtggg agacagagtg accatcacct gtcacagcag ccaggacatc      480

aacagcaaca tcggctggct gcagcagaag cccggcaagt cttttaaggg cctgatctac      540

cacggcacca acctggatga tggcgtgccc agcagatttt ctggcagcgg ctctggcacc      600

gactacaccc tgaccatatc tagcctgcag cctgaggact tcgccaccta ttactgcgtg      660

cagtacgccc agtttccttg gacctttgga ggcggcacaa agctggaaat caagcgg         717


<210>  80
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 806 scFv

<400>  80

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Tyr Ser Ile Ser Ser Asp 
            20                  25                  30          


Phe Ala Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Met Gly Tyr Ile Ser Tyr Ser Gly Asn Thr Arg Tyr Gln Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Ile Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Phe Phe 
65                  70                  75                  80  


Leu Lys Leu Asn Ser Val Thr Ala Ala Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Val Thr Ala Gly Arg Gly Phe Pro Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Gly 
        115                 120                 125             


Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Met Ser Val 
    130                 135                 140                 


Ser Val Gly Asp Arg Val Thr Ile Thr Cys His Ser Ser Gln Asp Ile 
145                 150                 155                 160 


Asn Ser Asn Ile Gly Trp Leu Gln Gln Lys Pro Gly Lys Ser Phe Lys 
                165                 170                 175     


Gly Leu Ile Tyr His Gly Thr Asn Leu Asp Asp Gly Val Pro Ser Arg 
            180                 185                 190         


Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser 
        195                 200                 205             


Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Val Gln Tyr Ala Gln 
    210                 215                 220                 


Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
225                 230                 235                 


<210>  81
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Humanized 806 scFv

<400>  81
ggcgaactaa aggtcgaaac acggcggagg tttccaggtt cctttgaccc gcatgacgtg       60

cgtcattatc caccgcttca ggagtccgac gtccgatcta taccagtccc acatcagcca      120

cggtctcggc gacggtcttt tagacgaccc gtgcggtagt aggtccaacc acggcaccat      180

ctagtccggg aattttctga acggcccgaa gacgacgtcg gtcggctaca acgacaacta      240

caggaccgac gacactgtcc actaccagtg agacagaggg tgcctgtgcc tgtacgacga      300

ccccgagaca cagtagacct atagtctcgg tggcggtctt ctaggcggag gcggtctagg      360

aggcggtggt ctcgattgcc actggtcaca agggacaggg gttatcccct tcggagacgg      420

tcgacagtgt gttatcatcc accgccatag ccgccgacag tgcgacaagt cgaagtcctt      480

cttgaccaag aacgaccaca gagacgacta ccactaggcc ctgaagtccg atccgaccat      540

agaccacaac ggcgacatcg actacatcgg gtaggtaagg tcaggaaacg gtcctccgac      600

agactaggtc aaggtccgct tcagcgacga ctacgacatc ggcctgtgcc atgtccagtc      660

cgagtcacaa accgatccga actggtccgg tcccggtctg agaacgtcga cttggac         717


<210>  82
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 806 scFv

<400>  82

Arg Lys Ile Glu Leu Lys Thr Gly Gly Gly Phe Thr Trp Pro Phe Gln 
1               5                   10                  15      


Ala Tyr Gln Val Cys Tyr Tyr Thr Ala Phe Asp Glu Pro Gln Leu Ser 
            20                  25                  30          


Ser Ile Thr Leu Thr Tyr Asp Thr Gly Ser Gly Ser Gly Ser Phe Arg 
        35                  40                  45              


Ser Pro Val Gly Asp Asp Leu Asn Thr Gly His Tyr Ile Leu Gly Lys 
    50                  55                  60                  


Phe Ser Lys Gly Pro Lys Gln Gln Leu Trp Gly Ile Asn Ser Asn Ile 
65                  70                  75                  80  


Asp Gln Ser Ser His Cys Thr Ile Thr Val Arg Asp Gly Val Ser Val 
                85                  90                  95      


Ser Met Ser Ser Pro Ser Gln Thr Met Gln Ile Asp Ser Gly Gly Gly 
            100                 105                 110         


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Val Thr Val 
        115                 120                 125             


Leu Thr Gly Gln Gly Trp Tyr Pro Phe Gly Arg Gly Ala Thr Val Cys 
    130                 135                 140                 


Tyr Tyr Thr Ala Thr Asp Ala Ala Thr Val Ser Asn Leu Lys Leu Phe 
145                 150                 155                 160 


Phe Gln Asn Lys Ser Thr Asp Arg Ser Ile Thr Ile Arg Ser Lys Leu 
                165                 170                 175     


Ser Pro Gln Tyr Arg Thr Asn Gly Ser Tyr Ser Ile Tyr Gly Met Trp 
            180                 185                 190         


Glu Leu Gly Lys Gly Pro Pro Gln Arg Ile Trp Asn Trp Ala Phe Asp 
        195                 200                 205             


Ser Ser Ile Ser Tyr Gly Ser Val Thr Cys Thr Leu Ser Leu Thr Gln 
    210                 215                 220                 


Ser Pro Lys Val Leu Gly Pro Gly Ser Glu Gln Leu Gln Val Gln 
225                 230                 235                 


<210>  83
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Affinity maturated humanized 806 scFv

<400>  83
gaggttcagc tgcaagagtc tggccctggc ctggtcaagc ctagccaaac actgagcctg       60

acctgtaccg tgtccggcta cagcatcagc agagacttcg cctggaactg gatcagacag      120

cctcctggca aaggactgga atggatgggc tacatcagct acaacggcaa caccagatac      180

cagcctagcc tgaagtcccg gatcaccatc tccagagaca ccagcaagaa ccagttcttc      240

ctgaagctga acagcgtgac agccgccgat accgccacct actattgtgt gacagccagc      300

agaggcttcc cctattgggg acagggaacc ctggtcacag ttagctctgg tggcggagga      360

tctggcggag gcggatcttc tggcggtggc tctgatatcc agatgacaca gagccccagc      420

agcatgtccg tgtccgtggg agacagagtg accatcacct gtcacagcag ccaggacatc      480

aacagcaaca tcggctggct gcagcagaag cccggcaagt cttttaaggg cctgatctac      540

cacggcacca acctggatga tggcgtgccc agcagatttt ctggcagcgg ctctggcacc      600

gactacaccc tgaccatatc tagcctgcag cctgaggact tcgccaccta ttactgcgtg      660

cagtacgccc agtttccttg gacctttgga ggcggcacaa agctggaaat caag            714


<210>  84
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affinity maturated humanized 806 scFv

<400>  84

Glu Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Tyr Ser Ile Ser Arg Asp 
            20                  25                  30          


Phe Ala Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Met Gly Tyr Ile Ser Tyr Asn Gly Asn Thr Arg Tyr Gln Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Ile Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Phe Phe 
65                  70                  75                  80  


Leu Lys Leu Asn Ser Val Thr Ala Ala Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Val Thr Ala Ser Arg Gly Phe Pro Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Gly 
        115                 120                 125             


Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Met Ser Val 
    130                 135                 140                 


Ser Val Gly Asp Arg Val Thr Ile Thr Cys His Ser Ser Gln Asp Ile 
145                 150                 155                 160 


Asn Ser Asn Ile Gly Trp Leu Gln Gln Lys Pro Gly Lys Ser Phe Lys 
                165                 170                 175     


Gly Leu Ile Tyr His Gly Thr Asn Leu Asp Asp Gly Val Pro Ser Arg 
            180                 185                 190         


Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser 
        195                 200                 205             


Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Val Gln Tyr Ala Gln 
    210                 215                 220                 


Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
225                 230                 235             


<210>  85
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Affinity maturated humanized 806 scFv

<400>  85
gaactaaagg tcgaaacacg gcggaggttt ccaggttcct ttgacccgca tgacgtgcgt       60

cattatccac cgcttcagga gtccgacgtc cgatctatac cagtcccaca tcagccacgg      120

tctcggcgac ggtcttttag acgacccgtg cggtagtagg tccaaccacg gcaccatcta      180

gtccgggaat tttctgaacg gcccgaagac gacgtcggtc ggctacaacg acaactacag      240

gaccgacgac actgtccact accagtgaga cagagggtgc ctgtgcctgt acgacgaccc      300

cgagacacag tagacctata gtctcggtgg cggtcttcta ggcggaggcg gtctaggagg      360

cggtggtctc gattgacact ggtcccaagg gacaggggtt atccccttcg gagacgaccg      420

acagtgtgtt atcatccacc gccatagccg ccgacagtgc gacaagtcga agtccttctt      480

gaccaagaac gaccacagag acctctacca ctaggccctg aagtccgatc cgaccataga      540

ccacaacggc aacatcgact acatcgggta ggtaaggtca ggaaacggtc ctccgacaga      600

ctaggtcaag gtccgcttca gagacgacta cgacatcggc ctgtgccatg tccagtccga      660

gtcacaaacc gatccgaact ggtccggtcc cggtctgaga acgtcgactt ggag            714


<210>  86
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affinity maturated humanized 806 scFv

<400>  86

Lys Ile Glu Leu Lys Thr Gly Gly Gly Phe Thr Trp Pro Phe Gln Ala 
1               5                   10                  15      


Tyr Gln Val Cys Tyr Tyr Thr Ala Phe Asp Glu Pro Gln Leu Ser Ser 
            20                  25                  30          


Ile Thr Leu Thr Tyr Asp Thr Gly Ser Gly Ser Gly Ser Phe Arg Ser 
        35                  40                  45              


Pro Val Gly Asp Asp Leu Asn Thr Gly His Tyr Ile Leu Gly Lys Phe 
    50                  55                  60                  


Ser Lys Gly Pro Lys Gln Gln Leu Trp Gly Ile Asn Ser Asn Ile Asp 
65                  70                  75                  80  


Gln Ser Ser His Cys Thr Ile Thr Val Arg Asp Gly Val Ser Val Ser 
                85                  90                  95      


Met Ser Ser Pro Ser Gln Thr Met Gln Ile Asp Ser Gly Gly Gly Ser 
            100                 105                 110         


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Val Thr Val Leu 
        115                 120                 125             


Thr Gly Gln Gly Trp Tyr Pro Phe Gly Arg Ser Ala Thr Val Cys Tyr 
    130                 135                 140                 


Tyr Thr Ala Thr Asp Ala Ala Thr Val Ser Asn Leu Lys Leu Phe Phe 
145                 150                 155                 160 


Gln Asn Lys Ser Thr Asp Arg Ser Ile Thr Ile Arg Ser Lys Leu Ser 
                165                 170                 175     


Pro Gln Tyr Arg Thr Asn Gly Asn Tyr Ser Ile Tyr Gly Met Trp Glu 
            180                 185                 190         


Leu Gly Lys Gly Pro Pro Gln Arg Ile Trp Asn Trp Ala Phe Asp Arg 
        195                 200                 205             


Ser Ile Ser Tyr Gly Ser Val Thr Cys Thr Leu Ser Leu Thr Gln Ser 
    210                 215                 220                 


Pro Lys Val Leu Gly Pro Gly Ser Glu Gln Leu Gln Val Glu 
225                 230                 235             


