                         SEQUENCE LISTING

<110>  Arc Bio, LLC
       Gourguechon, Stephane B.
 
<120>  COMPOSITIONS AND METHODS FOR NUCLEOTIDE MODIFICATION-BASED 
       DEPLETION

<130>  ARCB-013/01WO 329757-2076

<150>  62/831,302
<151>  2019-04-09

<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EcoKI site


<220>
<221>  N
<222>  (4)..(9)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (4)..(9)
<223>  n is a, c, g, or t

<400>  1
aacnnnnnng tgc                                                          13


<210>  2
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EcoKI site


<220>
<221>  N
<222>  (5)..(10)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (5)..(10)
<223>  n is a, c, g, or t

<400>  2
gcacnnnnnn gtt                                                          13


<210>  3
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BcgI site


<220>
<221>  N
<222>  (6)..(9)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (6)..(9)
<223>  n is a, c, g, or t

<400>  3
cgatcnnnnt gc                                                           12


<210>  4
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BsaBI site


<220>
<221>  N
<222>  (5)..(7)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (5)..(7)
<223>  n is a, c, g, or t

<400>  4
gatcnnnatc                                                              10


<210>  5
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nt.AlwI site


<220>
<221>  N
<222>  (6)..(10)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (6)..(10)
<223>  n is a, c, g, or t

<400>  5
ggatcnnnnn                                                              10


<210>  6
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AlwNI site


<220>
<221>  N
<222>  (4)..(5)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, or t

<400>  6
cagnncctgg                                                              10


<210>  7
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BslI site


<220>
<221>  N
<222>  (6)..(9)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (6)..(9)
<223>  n is a, c, g, or t

<400>  7
ccwggnnnng g                                                            11


<210>  8
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BstXI site


<220>
<221>  N
<222>  (6)..(9)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (6)..(9)
<223>  n is a, c, g, or t

<400>  8
ccaggnnnnt gg                                                           12


<210>  9
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PflMI site


<220>
<221>  N
<222>  (6)..(8)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (6)..(8)
<223>  n is a, c, g, or t

<400>  9
ccaggnnntg g                                                            11


<210>  10
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SfiI site


<220>
<221>  W
<222>  (5)..(5)
<223>  W is A or T

<220>
<221>  N
<222>  (8)..(9)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (8)..(9)
<223>  n is a, c, g, or t

<400>  10
ggccwggnng gcc                                                          13


<210>  11
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SfiI site


<220>
<221>  N
<222>  (5)..(9)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (5)..(9)
<223>  n is a, c, g, or t

<220>
<221>  W
<222>  (14)..(14)
<223>  W is A or T

<400>  11
ggccnnnnng gccwgg                                                       16


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AbaSI site


<220>
<221>  ghm
<222>  (1)..(1)
<223>  glucosylhydroxymethylcytosine modification

<220>
<221>  N
<222>  (2)..(24)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (2)..(24)
<223>  n is a, c, g, or t

<400>  12
cnnnnnnnnn nnnnnnnnnn nnnng                                             25


<210>  13
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AbaSI site


<220>
<221>  ghm
<222>  (1)..(1)
<223>  glucosylhydroxymethylcytosine

<220>
<221>  N
<222>  (2)..(23)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (2)..(23)
<223>  n is a, c, g, or t

<400>  13
cnnnnnnnnn nnnnnnnnnn nnng                                              24


<210>  14
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AbaSI site


<220>
<221>  ghm
<222>  (1)..(1)
<223>  glucosylhydroxymethylcytosine modification

<220>
<221>  N
<222>  (2)..(22)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (2)..(22)
<223>  n is a, c, g, or t

<400>  14
cnnnnnnnnn nnnnnnnnnn nng                                               23


<210>  15
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AbaSI site


<220>
<221>  ghm
<222>  (1)..(1)
<223>  glucosylhydroxymethylcytosine modification

<220>
<221>  N
<222>  (2)..(21)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (2)..(21)
<223>  n is a, c, g, or t

<400>  15
cnnnnnnnnn nnnnnnnnnn ng                                                22


<210>  16
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AbaSI site


<220>
<221>  N
<222>  (2)..(24)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (2)..(24)
<223>  n is a, c, g, or t

<220>
<221>  *
<222>  (25)..(25)
<223>  5-glucosylhydroxymethylcytosine, 5-hydroxymethylcytosine, 
       5-methylcytosine or cytosine

<400>  16
gnnnnnnnnn nnnnnnnnnn nnnnc                                             25


<210>  17
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AbaSI site


<220>
<221>  N
<222>  (2)..(23)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (2)..(23)
<223>  n is a, c, g, or t

<220>
<221>  *
<222>  (24)..(24)
<223>  5-glucosylhydroxymethylcytosine, 5-hydroxymethylcytosine, 
       5-methylcytosine or cytosine

<400>  17
gnnnnnnnnn nnnnnnnnnn nnnc                                              24


<210>  18
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AbaSI site


<220>
<221>  N
<222>  (2)..(22)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (2)..(22)
<223>  n is a, c, g, or t

<220>
<221>  *
<222>  (23)..(23)
<223>  5-glucosylhydroxymethylcytosine, 5-hydroxymethylcytosine, 
       5-methylcytosine or cytosine

<400>  18
gnnnnnnnnn nnnnnnnnnn nnc                                               23


<210>  19
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AbaSI site


<220>
<221>  N
<222>  (2)..(21)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (2)..(21)
<223>  n is a, c, g, or t

<220>
<221>  *
<222>  (22)..(22)
<223>  5-glucosylhydroxymethylcytosine, 5-hydroxymethylcytosine, 
       5-methylcytosine or cytosine

<400>  19
gnnnnnnnnn nnnnnnnnnn nc                                                22


<210>  20
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FspEI site


<220>
<221>  mC
<222>  (2)..(2)
<223>  5-methylcytosine or 5-hydroxymethylcytosine

<220>
<221>  N
<222>  (2)..(14)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (3)..(14)
<223>  n is a, c, g, or t

<400>  20
ccnnnnnnnn nnnn                                                         14


<210>  21
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FspEI site


<220>
<221>  N
<222>  (3)..(18)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (3)..(18)
<223>  n is a, c, g, or t

<400>  21
ggnnnnnnnn nnnnnnnn                                                     18


<210>  22
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LpnPI site


<220>
<221>  mC
<222>  (2)..(2)
<223>  5-methylcytosine or 5-hydroxymethylcytosine

<220>
<221>  D
<222>  (3)..(3)
<223>  D is A, G, or T

<220>
<221>  N
<222>  (5)..(14)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (5)..(14)
<223>  n is a, c, g, or t

<400>  22
ccdgnnnnnn nnnn                                                         14


<210>  23
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LpnPI site


<220>
<221>  H
<222>  (3)..(3)
<223>  H is A, C or T

<220>
<221>  N
<222>  (5)..(18)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (5)..(18)
<223>  n is a, c, g, or t

<400>  23
gghcnnnnnn nnnnnnnn                                                     18


<210>  24
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MspJI site


<220>
<221>  mC
<222>  (1)..(1)
<223>  5-methylcytosine or 5-hydroxymethylcytosine

<220>
<221>  N
<222>  (2)..(3)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  n is a, c, g, or t

<220>
<221>  R
<222>  (4)..(4)
<223>  R is A or G

<220>
<221>  N
<222>  (5)..(13)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (5)..(13)
<223>  n is a, c, g, or t

<400>  24
cnnrnnnnnn nnn                                                          13


<210>  25
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MspJI site


<220>
<221>  N
<222>  (2)..(3)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  n is a, c, g, or t

<220>
<221>  Y
<222>  (4)..(4)
<223>  Y is C or T

<220>
<221>  N
<222>  (5)..(17)
<223>  N is any nucleotide

<220>
<221>  misc_feature
<222>  (5)..(17)
<223>  n is a, c, g, or t

<400>  25
gnnynnnnnn nnnnnnn                                                      17


<210>  26
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence encoding crRNA

<400>  26
gttttagagc tatgctgttt tg                                                22


<210>  27
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence encoding tracrRNA

<400>  27
ggaaccattc aaaacagcat agcaagttaa aataaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttttt                                            86


<210>  28
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gNA sequence

<400>  28
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt ttt                                               83


<210>  29
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gNA sequence

<400>  29
aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa       60

cttgctattt ctagctctaa aac                                               83


<210>  30
<211>  83
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  30
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugcuuuu uuu                                               83


<210>  31
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gNA sequence

<400>  31
gttttagagc tatgctggaa acagcatagc aagttaaaat aaggctagtc cgttatcaac       60

ttgaaaaagt ggcaccgagt cggtgctttt tttc                                   94


<210>  32
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gNA sequence

<400>  32
gaaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta       60

acttgctatg ctgtttccag catagctcta aaac                                   94


<210>  33
<211>  94
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  33
guuuuagagc uaugcuggaa acagcauagc aaguuaaaau aaggcuaguc cguuaucaac       60

uugaaaaagu ggcaccgagu cggugcuuuu uuuc                                   94


<210>  34
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  34
aauuucuacu guuguagau                                                    19


<210>  35
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gNA sequence

<400>  35
aatttctact gttgtagat                                                    19


<210>  36
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  36
guuuuagagc uaugcuguuu ug                                                22


<210>  37
<211>  86
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  37
ggaaccauuc aaaacagcau agcaaguuaa aauaaggcua guccguuauc aacuugaaaa       60

aguggcaccg agucggugcu uuuuuu                                            86


