                         SEQUENCE LISTING

<110>  Agency for Science, Technology and Research
 
<120>  A RIBOZYME COMPRISING A TARGET-BINDING DOMAIN

<130>  9869SG5876

<150>  SG 10201903323U
<151>  2019-04-12

<160>  102   

<170>  PatentIn version 3.5

<210>  1
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Sequence of exemplary target RNA dme-bantam-5p

<400>  1
ccgguuuucg auuugguuug acu                                               23


<210>  2
<211>  181
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Resulting S1 strand for dme-ban-5p ribozyme (S1_Tban5p_Cban3pR)

<400>  2
gggaagucaa accaaguaua uuaguauauu accugguuuu cgaucgaaag aucgacgagg       60

ugaaaaccuc gugacagucc uuagucgaaa guuuuacuag agucaguccu gauugugcuc      120

gaaagagcac agaccugaaa aggucuuucg ugguauauua cucucugaaa ucgaaaaccg      180

g                                                                      181


<210>  3
<211>  109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Resulting S2 strand for dme-ban-5p ribozyme (S2_Tban5p_Cban3pR)

<400>  3
gggaagucaa accaaguaau auaagaaaca cacgaaucag agaagacgag cgucccacgg       60

gcgcagaaga gaagucaacc agagaaacau aauauacauc gaaaaccgg                  109


<210>  4
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Sequence of exemplary target RNA hsa-let-7f-5p

<400>  4
ugagguagua gauuguauag uu                                                22


<210>  5
<211>  180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Resulting S1 strand for hsa-let-7f-5p ribozyme 
       (S1_Tlet7f_Cban3pR)

<400>  5
gggaaacuau acaauguaua uuaguauauu accugguuuu cgaucgaaag aucgacgagg       60

ugaaaaccuc gugacagucc uuagucgaaa guuuuacuag agucaguccu gauugugcuc      120

gaaagagcac agaccugaaa aggucuuucg ugguauauua cucucugaac uacuaccuca      180


<210>  6
<211>  108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Resulting S2 strand for hsa-let-7f-5p ribozyme 
       (S2_Tlet7f_Cban3pR)

<400>  6
gggaaacuau acaauguaau auaagaaaca cacgaaucag agaagacgag cgucccacgg       60

gcgcagaaga gaagucaacc agagaaacau aauauaccua cuaccuca                   108


<210>  7
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 promoter sequence

<400>  7
gtataatacg actcactata ggga                                              24


<210>  8
<211>  156
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S1 sequence with an exemplary cleavage product, and (n)a and (n)b
       represent sequences complementary to the target  RNA molecule.


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  (n)a represents the first sequence complementary to the target 
       RNA molecule.

<220>
<221>  misc_feature
<222>  (63)..(91)
<223>  Exemplary cleavage product SEQ ID NO. 25

<220>
<221>  misc_feature
<222>  (156)..(156)
<223>  (n)b represents the second sequence complementary to the target 
       RNA molecule.

<400>  8
nguauauuag uauauuaccu gguuuucgau cgaaagaucg acgaggugaa aaccucguga       60

caguccuuag ucgaaaguuu uacuagaguc aguccugauu gugcucgaaa gagcacagac      120

cugaaaaggu cuuucguggu auauuacucu cugaan                                156


<210>  9
<211>  84
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S2 sequence. (n)a and (n)b represent sequences complementary to 
       the target  RNA molecule.


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  (n)a represents the first sequence complementary to the target 
       RNA molecule.

<220>
<221>  misc_feature
<222>  (84)..(84)
<223>  (n)b represents the second sequence complementary to the target 
       RNA molecule.

<400>  9
nguaauauaa gaaacacacg aaucagagaa gacgagcguc ccacgggcgc agaagagaag       60

ucaaccagag aaacauaaua uacn                                              84


<210>  10
<211>  98
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Core S1 DNA template

<400>  10
ctggttttcg atcgaaagat cgacgaggtg aaaacctcgt gacagtcctt agtcgaaagt       60

tttactagag tcagtcctga ttgtgctcga aagagcac                               98


<210>  11
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Core S2 DNA template

<400>  11
agaaacacac gaatcagaga agacgagcgt cccacgggcg cagaagagaa gtca             54


<210>  12
<211>  12
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Reverse complement of 5' paired sequence of dme-ban-5p

<400>  12
aucgaaaacc gg                                                           12


<210>  13
<211>  11
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Reverse complement of 3' paired sequence of dme-ban-5p

<400>  13
agucaaacca a                                                            11


<210>  14
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S1 forward primer for dme-ban-5p


<220>
<221>  promoter
<222>  (1)..(24)
<223>  T7 promoter sequence

<400>  14
gtataatacg actcactata gggaagtcaa accaagtata ttagtatatt acctggtttt       60

cgatcgaaag atc                                                          73


<210>  15
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S1 reverse primer for dme-ban-5p

<400>  15
ccggttttcg atttcagaga gtaatatacc acgaaagacc ttttcaggtc tgtgctcttt       60

cgagcacaat c                                                            71


<210>  16
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S2 forward primer for dme-ban-5p


<220>
<221>  promoter
<222>  (1)..(24)
<223>  T7 promoter sequence

<400>  16
gtataatacg actcactata gggaagtcaa accaagtaat ataagaaaca cacgaatcag       60

agaa                                                                    64


<210>  17
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S2 reverse primer for dme-ban-5p

<400>  17
ccggttttcg atgtatatta tgtttctctg gttgacttct cttctgcg                    48


<210>  18
<211>  11
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Reverse complement of 5' paired sequence of hsa-let-7f-5p

<400>  18
cuacuaccuc a                                                            11


<210>  19
<211>  11
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Reverse complement of 3' paired sequence of hsa-let-7f-5p

<400>  19
aacuauacaa u                                                            11


<210>  20
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S1 forward primer for hsa-let-7f-5p


<220>
<221>  promoter
<222>  (1)..(24)
<223>  T7 promoter sequence

<400>  20
gtataatacg actcactata gggaaactat acaatgtata ttagtatatt acctggtttt       60

cgatcgaaag atc                                                          73


<210>  21
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S1 reverse primer for hsa-let-7f-5p

<400>  21
tgaggtagta gttcagagag taatatacca cgaaagacct tttcaggtct gtgctctttc       60

gagcacaatc                                                              70


<210>  22
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S2 forward primer for hsa-let-7f-5p


<220>
<221>  promoter
<222>  (1)..(24)
<223>  T7 promoter sequence

<400>  22
gtataatacg actcactata gggaaactat acaatgtaat ataagaaaca cacgaatcag       60

agaa                                                                    64


<210>  23
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S2 reverse primer for hsa-let-7f-5p

<400>  23
tgaggtagta ggtatattat gtttctctgg ttgacttctc ttctgcg                     47


<210>  24
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hsa-miR-122-5p, a control RNA molecule with a dissimilar sequence
       to a target RNA molecule to test sequence specificity of the 
       ribozymes

<400>  24
uggaguguga caaugguguu ug                                                22


<210>  25
<211>  29
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Exemplary cleavage product of SEQ ID NO. 8.

<400>  25
guccuuaguc gaaaguuuua cuagaguca                                         29


<210>  26
<211>  134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  An exemplary  S1 sequence with toeholds. (n)x represents a 
       releasable RNA segment, and (n)a and (n)b represent sequences 
       complementary to the first and second target  RNA molecules.


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  (n)a represents the first sequence complementary to the target 
       RNA molecule.

<220>
<221>  misc_feature
<222>  (67)..(67)
<223>  (n)x represents a releasable RNA segment.

<220>
<221>  misc_feature
<222>  (134)..(134)
<223>  (n)b represents the second sequence complementary to the target 
       RNA molecule.

<400>  26
nguauauuag uauauuaccu gguuuucgau cgaaagaucg acgaggugaa aaccucguga       60

caguccncag uccugauugu gcucgaaaga gcacagaccu gaaaaggucu uucgugguau      120

auuacucucu gaan                                                        134


<210>  27
<211>  65
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Portion of SEQ ID NO.: 26 between (n)a (the first sequence 
       complementary to the target  RNA molecule and (n)x (the 
       releasable RNA segment).

<400>  27
guauauuagu auauuaccug guuuucgauc gaaagaucga cgaggugaaa accucgugac       60

agucc                                                                   65


<210>  28
<211>  66
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Portion of SEQ ID NO.: 26 between (n)x (the cleavage product) and
       (n)b (the second sequence complementary to the target RNA 
       molecule).

<400>  28
caguccugau ugugcucgaa agagcacaga ccugaaaagg ucuuucgugg uauauuacuc       60

ucugaa                                                                  66


<210>  29
<211>  62
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  An exemplary S2 sequence (with toeholds). (n)x represents a 
       region partially complementary to the releasable RNA segment on 
       S1, and (n)a and (n)b represent sequences complementary to the 
       second and first target RNA molecules.


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  (n)a represents the first sequence complementary to the target 
       RNA molecule.

<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  (n)x represents a region partially complementary to the 
       releasable RNA segment on S1.

<220>
<221>  misc_feature
<222>  (62)..(62)
<223>  (n)b represents the second sequence complementary to the target 
       RNA molecule.

<400>  29
nguaauauaa gaaacacacg aaucagagaa gngagaaguc aaccagagaa acauaauaua       60

cn                                                                      62


<210>  30
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Portion of SEQ ID NO.: 29 between (n)a (the sequence 
       complementary to the second target RNA molecule and (n)x (the 
       region partially complementary to the releasable RNA segment).

<400>  30
guaauauaag aaacacacga aucagagaag                                        30


<210>  31
<211>  29
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Portion of SEQ ID NO.: 29 between (n)x (the region partially 
       complementary to the releasable RNA segment) and (n)b (the 
       sequence complementary to the second target RNA molecule).

<400>  31
gagaagucaa ccagagaaac auaauauac                                         29


<210>  32
<211>  154
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  An exemplary sequence of the region of S1 between motifs [A] and 
       [A'], in the direction of [A] to [A'].

<400>  32
guauauuagu auauuaccug guuuucgauc gaaagaucga cgaggugaaa accucgugac       60

aguccuuagu cgaaaguuuu acuagaguca guccugauug ugcucgaaag agcacagacc      120

ugaaaagguc uuucguggua uauuacucuc ugaa                                  154


<210>  33
<211>  82
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  An exemplary sequence of the region of S2 between motifs [a'] and
       [a], in the direction of [a'] to [a].

<400>  33
guaauauaag aaacacacga aucagagaag acgagcgucc cacgggcgca gaagagaagu       60

caaccagaga aacauaauau ac                                                82


<210>  34
<211>  107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  An exemplary sequence of the region of S1 between motifs [B] and 
       [B'], in the direction of [B] to [B'].


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  Catalytic domain

<400>  34
guauauuacc ugguuuucga ucgaaagauc gacgagguga aaaccucgug acaguccuua       60

gucgaaaguu uuacuagagu caguccugau ugugcucgaa agagcac                    107


<210>  35
<211>  66
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  An exemplary sequence of the region of S2 between motifs [b'] and
       [b], in the direction of [b'] to [b].


<220>
<221>  misc_feature
<222>  (55)..(66)
<223>  Catalytic domain

<400>  35
agaaacacac gaaucagaga agacgagcgu cccacgggcg cagaagagaa gucaaccaga       60

gaaaca                                                                  66


<210>  36
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S1 forward primer for dme-ban-5p without the T7 promoter sequence

<400>  36
agtcaaacca agtatattag tatattacct ggttttcgat cgaaagatc                   49


<210>  37
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S2 forward primer for dme-ban-5p without T7 promoter sequence

<400>  37
agtcaaacca agtaatataa gaaacacacg aatcagagaa                             40


<210>  38
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S1 forward primer for hsa-let-7f-5p without T7 promoter sequence

<400>  38
aactatacaa tgtatattag tatattacct ggttttcgat cgaaagatc                   49


<210>  39
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S2 forward primer for hsa-let-7f-5p without T7 promoter sequence

<400>  39
aactatacaa tgtaatataa gaaacacacg aatcagagaa                             40


<210>  40
<211>  40
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Target RNA T7_Orf1ab_ 20


<220>
<221>  promoter
<222>  (1)..(24)
<223>  T7 promoter sequence

<400>  40
guauaauacg acucacuaua gggacagauc uaauggcugc                             40


<210>  41
<211>  174
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S1_Orf1ab _20

<400>  41
gcagccauua guauauuagu auauuaccug guuuucgauc gaaagaucga cgaggugaaa       60

accucgugac aguccuuagu cgaaaguuuu acuagaguca guccugauug ugcucgaaag      120

agcacagacc ugaaaagguc uuucguggua uauuacucuc ugaagaucug uccc            174


<210>  42
<211>  102
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S2_Orf1ab_20

<400>  42
gcagccauua guaauauaag aaacacacga aucagagaag acgagcgucc cacgggcgca       60

gaagagaagu caaccagaga aacauaauau acgaucuguc cc                         102


<210>  43
<211>  60
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Target RNA T7_Orf1ab_ 40


<220>
<221>  promoter
<222>  (1)..(24)
<223>  T7 promoter sequence

<400>  43
guauaauacg acucacuaua gggaguagac aauucuaguc uuacuauuaa gaaaccuaau       60


<210>  44
<211>  194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S1_Orf1ab _40

<400>  44
auuagguuuc uuaauaguaa guauauuagu auauuaccug guuuucgauc gaaagaucga       60

cgaggugaaa accucgugac aguccuuagu cgaaaguuuu acuagaguca guccugauug      120

ugcucgaaag agcacagacc ugaaaagguc uuucguggua uauuacucuc ugaagacuag      180

aauugucuac uccc                                                        194


<210>  45
<211>  122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S2_Orf1ab_40

<400>  45
auuagguuuc uuaauaguaa guaauauaag aaacacacga aucagagaag acgagcgucc       60

cacgggcgca gaagagaagu caaccagaga aacauaauau acgacuagaa uugucuacuc      120

cc                                                                     122


<210>  46
<211>  80
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  T7_Egene_60


<220>
<221>  promoter
<222>  (1)..(24)
<223>  T7 promoter sequence

<400>  46
guauaauacg acucacuaua gggacugcgc uucgauugug ugcguacugc ugcaauauug       60

uuaacgugag ucuuguaaaa                                                   80


<210>  47
<211>  214
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S1_E_gene_60

<400>  47
uuuuacaaga cucacguuaa caauauugca guauauuagu auauuaccug guuuucgauc       60

gaaagaucga cgaggugaaa accucgugac aguccuuagu cgaaaguuuu acuagaguca      120

guccugauug ugcucgaaag agcacagacc ugaaaagguc uuucguggua uauuacucuc      180

ugaagcagua cgcacacaau cgaagcgcag uccc                                  214


<210>  48
<211>  142
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S2_Egene_60

<400>  48
uuuuacaaga cucacguuaa caauauugca guaauauaag aaacacacga aucagagaag       60

acgagcgucc cacgggcgca gaagagaagu caaccagaga aacauaauau acgcaguacg      120

cacacaaucg aagcgcaguc cc                                               142


<210>  49
<211>  16
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Target RNA T7_Orf1ab_ 20 without T7 promoter sequence

<400>  49
cagaucuaau ggcugc                                                       16


<210>  50
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Target RNA T7_Orf1ab_ 40 without T7 promoter sequence

<400>  50
guagacaauu cuagucuuac uauuaagaaa ccuaau                                 36


<210>  51
<211>  56
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  T7_Egene_60 without T7 promoter sequence

<400>  51
cugcgcuucg auugugugcg uacugcugca auauuguuaa cgugagucuu guaaaa           56


<210>  52
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S1 forward primer for S1_Orf1ab_gene_20_F


<220>
<221>  promoter
<222>  (1)..(24)
<223>  T7 promoter sequence

<400>  52
gtataatacg actcactata gggagcagcc attagtatat tagtatatta cctggttttc       60

gatcgaaaga tc                                                           72


<210>  53
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S1 reverse primer S1_Orf1ab_gene_20_R

<400>  53
gggacagatc ttcagagagt aatataccac gaaagacctt ttcaggtctg tgctctttcg       60

agcacaatc                                                               69


<210>  54
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S2 forward primer S2_Orf1ab_gene_20_F


<220>
<221>  promoter
<222>  (1)..(24)
<223>  T7 promoter sequence

<400>  54
gtataatacg actcactata gggagcagcc attagtaata taagaaacac acgaatcaga       60

gaa                                                                     63


<210>  55
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S2 reverse primer S2_Orf1ab_gene_20_R

<400>  55
gggacagatc gtatattatg tttctctggt tgacttctct tctgcg                      46


<210>  56
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S1 forward primer S1_Orf1ab_gene_40_F


<220>
<221>  promoter
<222>  (1)..(24)
<223>  T7 promoter sequence

<400>  56
gtataatacg actcactata gggaattagg tttcttaata gtaagtatat tagtatatta       60

cctggttttc gatcgaaaga tc                                                82


<210>  57
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S1 reverse primer S1_Orf1ab_gene_40_R

<400>  57
gggagtagac aattctagtc ttcagagagt aatataccac gaaagacctt ttcaggtctg       60

tgctctttcg agcacaatc                                                    79


<210>  58
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S2 forward primer S2_Orf1ab_gene_40_F


<220>
<221>  promoter
<222>  (1)..(24)
<223>  T7 promoter sequence

<400>  58
gtataatacg actcactata gggaattagg tttcttaata gtaagtaata taagaaacac       60

acgaatcaga gaa                                                          73


<210>  59
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S2 reverse primer S2_Orf1ab_gene_40_R

<400>  59
gggagtagac aattctagtc gtatattatg tttctctggt tgacttctct tctgcg           56


<210>  60
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S1 forward primer S1_E_gene_60_F


<220>
<221>  promoter
<222>  (1)..(24)
<223>  T7 promoter sequence

<400>  60
gtataatacg actcactata gggattttac aagactcacg ttaacaatat tgcagtatat       60

tagtatatta cctggttttc gatcgaaaga tc                                     92


<210>  61
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S1 reverse primer S1_E_gene_60_R

<400>  61
gggactgcgc ttcgattgtg tgcgtactgc ttcagagagt aatataccac gaaagacctt       60

ttcaggtctg tgctctttcg agcacaatc                                         89


<210>  62
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S2 forward primer S2_E_gene_60_F


<220>
<221>  promoter
<222>  (1)..(24)
<223>  T7 promoter sequence

<400>  62
gtataatacg actcactata gggattttac aagactcacg ttaacaatat tgcagtaata       60

taagaaacac acgaatcaga gaa                                               83


<210>  63
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S2 reverse primer S2_E_gene_60_R

<400>  63
gggactgcgc ttcgattgtg tgcgtactgc gtatattatg tttctctggt tgacttctct       60

tctgcg                                                                  66


<210>  64
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S1 forward primer for S1_Orf1ab_gene_20_F without T7 promoter 
       sequence

<400>  64
gcagccatta gtatattagt atattacctg gttttcgatc gaaagatc                    48


<210>  65
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S2 forward primer S2_Orf1ab_gene_20_F without T7 promoter 
       sequence

<400>  65
gcagccatta gtaatataag aaacacacga atcagagaa                              39


<210>  66
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S1 forward primer S1_Orf1ab_gene_40_F without T7 promoter 
       sequence

<400>  66
attaggtttc ttaatagtaa gtatattagt atattacctg gttttcgatc gaaagatc         58


<210>  67
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S2 forward primer S2_Orf1ab_gene_40_F without T7 promoter 
       sequence

<400>  67
attaggtttc ttaatagtaa gtaatataag aaacacacga atcagagaa                   49


<210>  68
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S1 forward primer S1_E_gene_60_F without T7 promoter sequence

<400>  68
ttttacaaga ctcacgttaa caatattgca gtatattagt atattacctg gttttcgatc       60

gaaagatc                                                                68


<210>  69
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S2 forward primer S2_E_gene_60_F without T7 promoter sequence

<400>  69
ttttacaaga ctcacgttaa caatattgca gtaatataag aaacacacga atcagagaa        59


<210>  70
<211>  181
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S1 strand of dme-ban-5p without toehold

<400>  70
gggaagucaa accaagggaa gugguauauu accugguuuu cgaucgaaag aucgacgagg       60

ugaaaaccuc gugacagucc uuagucgaaa guuuuacuag agucaguccu gauugugcuc      120

gaaagagcac agaccugaaa aggucuuucg ugguauauua ccugguccca ucgaaaaccg      180

g                                                                      181


<210>  71
<211>  109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S2 strand of dme-ban-5p without toehold

<400>  71
gggaagucaa accaagggac cagagaaaca cacgaaucag agaagacgag cgucccacgg       60

gcgcagaaga gaagucaacc agagaaacac acuucccauc gaaaaccgg                  109


<210>  72
<211>  180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S1 strand of hsa-let-7f-5p without toehold

<400>  72
gggaaacuau acaaugggaa gugguauauu accugguuuu cgaucgaaag aucgacgagg       60

ugaaaaccuc gugacagucc uuagucgaaa guuuuacuag agucaguccu gauugugcuc      120

gaaagagcac agaccugaaa aggucuuucg ugguauauua ccuggucccc uacuaccuca      180


<210>  73
<211>  108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S2 strand of hsa-let-7f-5p without toehold

<400>  73
gggaaacuau acaaugggac cagagaaaca cacgaaucag agaagacgag cgucccacgg       60

gcgcagaaga gaagucaacc agagaaacac acuuccccua cuaccuca                   108


<210>  74
<211>  174
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S1 strand of Orf1ab_gene_20nt without toehold

<400>  74
gcagccauua gggaaguggu auauuaccug guuuucgauc gaaagaucga cgaggugaaa       60

accucgugac aguccuuagu cgaaaguuuu acuagaguca guccugauug ugcucgaaag      120

agcacagacc ugaaaagguc uuucguggua uauuaccugg ucccgaucug uccc            174


<210>  75
<211>  102
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S2 strand of Orf1ab_gene_20nt without toehold

<400>  75
gcagccauua gggaccagag aaacacacga aucagagaag acgagcgucc cacgggcgca       60

gaagagaagu caaccagaga aacacacuuc ccgaucuguc cc                         102


<210>  76
<211>  194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S1strand of Orf1ab_gene_40nt without toehold

<400>  76
auuagguuuc uuaauaguaa gggaaguggu auauuaccug guuuucgauc gaaagaucga       60

cgaggugaaa accucgugac aguccuuagu cgaaaguuuu acuagaguca guccugauug      120

ugcucgaaag agcacagacc ugaaaagguc uuucguggua uauuaccugg ucccgacuag      180

aauugucuac uccc                                                        194


<210>  77
<211>  122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S2 strand of Orf1ab_gene_40nt without toehold

<400>  77
auuagguuuc uuaauaguaa gggaccagag aaacacacga aucagagaag acgagcgucc       60

cacgggcgca gaagagaagu caaccagaga aacacacuuc ccgacuagaa uugucuacuc      120

cc                                                                     122


<210>  78
<211>  214
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S1 strand of E_gene_60nt without toehold

<400>  78
uuuuacaaga cucacguuaa caauauugca gggaaguggu auauuaccug guuuucgauc       60

gaaagaucga cgaggugaaa accucgugac aguccuuagu cgaaaguuuu acuagaguca      120

guccugauug ugcucgaaag agcacagacc ugaaaagguc uuucguggua uauuaccugg      180

ucccgcagua cgcacacaau cgaagcgcag uccc                                  214


<210>  79
<211>  142
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S2 strand of E_gene_60nt without toehold

<400>  79
uuuuacaaga cucacguuaa caauauugca gggaccagag aaacacacga aucagagaag       60

acgagcgucc cacgggcgca gaagagaagu caaccagaga aacacacuuc ccgcaguacg      120

cacacaaucg aagcgcaguc cc                                               142


<210>  80
<211>  134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  An exemplary S1 sequence without toeholds. (n)x represents a 
       region partially complementary to the releasable RNA segment on 
       S1, and (n)a and (n)b represent sequences complementary to the 
       target miRNA.


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  (n)a represents the first sequence complementary to the target 
       RNA molecule.

<220>
<221>  misc_feature
<222>  (67)..(67)
<223>  (n)x represents a region partially complementary to the 
       releasable RNA segment on S1.

<220>
<221>  misc_feature
<222>  (134)..(134)
<223>  (n)b represents the second sequence complementary to the target 
       RNA molecule.

<400>  80
ngggaagugg uauauuaccu gguuuucgau cgaaagaucg acgaggugaa aaccucguga       60

caguccncag uccugauugu gcucgaaaga gcacagaccu gaaaaggucu uucgugguau      120

auuaccuggu cccn                                                        134


<210>  81
<211>  65
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Portion of SEQ ID NO.: 80 between (n)a (the first sequence 
       complementary to the target  RNA molecule and (n)x (the 
       releasable RNA segment).

<400>  81
gggaaguggu auauuaccug guuuucgauc gaaagaucga cgaggugaaa accucgugac       60

agucc                                                                   65


<210>  82
<211>  66
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Portion of SEQ ID NO.: 80 between (n)x (the cleavage product) and
       (n)b (the second sequence complementary to the target RNA 
       molecule).

<400>  82
caguccugau ugugcucgaa agagcacaga ccugaaaagg ucuuucgugg uauauuaccu       60

gguccc                                                                  66


<210>  83
<211>  62
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  An exemplary S2 sequence (without toeholds). (n)x represents a 
       region partially complementary to the releasable RNA segment on 
       S1, and (n)a and (n)b represent sequences complementary to the 
       second and first target RNA molecules.


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  (n)a represents the first sequence complementary to the target 
       RNA molecule.

<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  (n)x represents a region partially complementary to the 
       releasable RNA segment on S1.

<220>
<221>  misc_feature
<222>  (62)..(62)
<223>  (n)b represents the second sequence complementary to the target 
       RNA molecule.

<400>  83
ngggaccaga gaaacacacg aaucagagaa gngagaaguc aaccagagaa acacacuucc       60

cn                                                                      62


<210>  84
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Portion of SEQ ID NO.: 83 between (n)a (the sequence 
       complementary to the second target RNA molecule and (n)x (the 
       region partially complementary to the releasable RNA segment).

<400>  84
gggaccagag aaacacacga aucagagaag                                        30


<210>  85
<211>  29
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Portion of SEQ ID NO.: 83  between (n)x (the region partially 
       complementary to the releasable RNA segment) and (n)b (the 
       sequence complementary to the second target RNA molecule).

<400>  85
gagaagucaa ccagagaaac acacuuccc                                         29


<210>  86
<211>  237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Tban5p_Cban3pR_singledesign1_full_length


<220>
<221>  misc_feature
<222>  (5)..(15)
<223>  sequence complementary to target RNA

<220>
<221>  misc_feature
<222>  (81)..(103)
<223>  Releasable RNA segment

<220>
<221>  misc_feature
<222>  (174)..(196)
<223>  Sequence partially complementary to the releasable RNA segment

<220>
<221>  misc_feature
<222>  (226)..(237)
<223>  Sequence complementary to target RNA

<400>  86
gggaagucaa accaaguaua uuaguauauu accugguuuu cgaucgaaag aucgacgagg       60

ugaaaaccuc gugacagucc uuagucgaaa guuuuacuag agucaguccu gauugugauc      120

gaaagagcac agaccugaaa aggucuuucu gguuucgacc agaaucagag aagacgagcg      180

ucccacgggc gcagaagaga agucaaccag agaaacauaa uauacaucga aaaccgg         237


<210>  87
<211>  193
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Tban5p_Cban3pR_singledesign2_full_length


<220>
<221>  misc_feature
<222>  (5)..(15)
<223>  Sequence complementary to target RNA molecule

<220>
<221>  misc_feature
<222>  (81)..(103)
<223>  Releasable RNA segment

<220>
<221>  misc_feature
<222>  (130)..(152)
<223>  Sequence partially complementary to the releasable RNA segment

<220>
<221>  misc_feature
<222>  (182)..(193)
<223>  Sequence complementary to target RNA molecule

<400>  87
gggaagucaa accaaguaua uuaguauauu accugguuuu cgaucgaaag aucgacgagg       60

ugaaaaccuc gugacagucc uuagucgaaa guuuuacuag agucaguccu gauuuucgaa      120

ucagagaaga cgagcguccc acgggcgcag aagagaaguc aaccagagaa acauaauaua      180

caucgaaaac cgg                                                         193


<210>  88
<211>  168
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  An exemplary sequence of a single strand ribozyme.


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  (n)a represent sequences complementary to the first region  of 
       the target  RNA molecule

<220>
<221>  misc_feature
<222>  (67)..(67)
<223>  (n)x represents a releasable RNA segment

<220>
<221>  misc_feature
<222>  (138)..(138)
<223>  (n)y represents a sequence optionally complementary to the 
       releasable RNA segment

<220>
<221>  misc_feature
<222>  (168)..(168)
<223>  (n)b represent sequences complementary to the second region  of 
       the target  RNA molecule

<400>  88
nguauauuag uauauuaccu gguuuucgau cgaaagaucg acgaggugaa aaccucguga       60

caguccncag uccugauugu gaucgaaaga gcacagaccu gaaaaggucu uucugguuuc      120

gaccagaauc agagaagnga gaagucaacc agagaaacau aauauacn                   168


<210>  89
<211>  65
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Portion of SEQ ID NO.: 88 between (n)a and (n)x

<400>  89
guauauuagu auauuaccug guuuucgauc gaaagaucga cgaggugaaa accucgugac       60

agucc                                                                   65


<210>  90
<211>  70
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Portion of SEQ ID NO.: 88 between (n)x and (n)y

<400>  90
caguccugau ugugaucgaa agagcacaga ccugaaaagg ucuuucuggu uucgaccaga       60

aucagagaag                                                              70


<210>  91
<211>  29
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Portion of SEQ ID NO.: 88 between (n)y and (n)b

<400>  91
gagaagucaa ccagagaaac auaauauac                                         29


<210>  92
<211>  183
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S1_Tban5p_Cban3pR_Dup

<400>  92
gggaagucaa accaaguaua uuaguauauu accugguuuu cgaucgaaag aucgacgagg       60

ugaaaaccuc gugacagucc uuagucgaaa guuuuacuag agucagucca gaaaucuccc      120

ucacuucggu gagggagauu ucugagaaga caccagagaa acauaauaua caucgaaaac      180

cgg                                                                    183


<210>  93
<211>  95
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S2_Tban5p_Cban3pR_Dup

<400>  93
gggaagucaa accaaguaua uuaguauauu accugguguc ccacgggcgc agaagagaag       60

ucaaccagag aaacauaaua uacaucgaaa accgg                                  95


<210>  94
<211>  182
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S1_Tlet7f_Cban3pR_Dup

<400>  94
gggaaacuau acaauguaua uuaguauauu accugguuuu cgaucgaaag aucgacgagg       60

ugaaaaccuc gugacagucc uuagucgaaa guuuuacuag agucagucca gaaaucuccc      120

ucacuucggu gagggagauu ucugagaaga caccagagaa acauaauaua ccuacuaccu      180

ca                                                                     182


<210>  95
<211>  94
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S2_Tlet7f_Cban3pR_Dup

<400>  95
gggaaacuau acaauguaua uuaguauauu accugguguc ccacgggcgc agaagagaag       60

ucaaccagag aaacauaaua uaccuacuac cuca                                   94


<210>  96
<211>  180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S1_T_Orf1ab_20_Cban3pR_Dup

<400>  96
gggagcagcc auuaguauau uaguauauua ccugguuuuc gaucgaaaga ucgacgaggu       60

gaaaaccucg ugacaguccu uagucgaaag uuuuacuaga gucaguccag aaaucucccu      120

cacuucggug agggagauuu cugagaagac accagagaaa cauaauauac gaucuguccc      180


<210>  97
<211>  92
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S2_T_Orf1ab_20_Cban3pR_Dup

<400>  97
gggagcagcc auuaguauau uaguauauua ccuggugucc cacgggcgca gaagagaagu       60

caaccagaga aacauaauau acgaucuguc cc                                     92


<210>  98
<211>  200
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S1_T_Orf1ab_40_Cban3pR_Dup

<400>  98
gggaauuagg uuucuuaaua guaaguauau uaguauauua ccugguuuuc gaucgaaaga       60

ucgacgaggu gaaaaccucg ugacaguccu uagucgaaag uuuuacuaga gucaguccag      120

aaaucucccu cacuucggug agggagauuu cugagaagac accagagaaa cauaauauac      180

gacuagaauu gucuacuccc                                                  200


<210>  99
<211>  112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S2_T_Orf1ab_40_Cban3pR_Dup

<400>  99
gggaauuagg uuucuuaaua guaaguauau uaguauauua ccuggugucc cacgggcgca       60

gaagagaagu caaccagaga aacauaauau acgacuagaa uugucuacuc cc              112


<210>  100
<211>  220
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S1_T_Egene_60_Cban3pR_Dup

<400>  100
gggauuuuac aagacucacg uuaacaauau ugcaguauau uaguauauua ccugguuuuc       60

gaucgaaaga ucgacgaggu gaaaaccucg ugacaguccu uagucgaaag uuuuacuaga      120

gucaguccag aaaucucccu cacuucggug agggagauuu cugagaagac accagagaaa      180

cauaauauac gcaguacgca cacaaucgaa gcgcaguccc                            220


<210>  101
<211>  132
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  S2_T_Egene_60_Cban3pR_Dup

<400>  101
gggauuuuac aagacucacg uuaacaauau ugcaguauau uaguauauua ccuggugucc       60

cacgggcgca gaagagaagu caaccagaga aacauaauau acgcaguacg cacacaaucg      120

aagcgcaguc cc                                                          132


<210>  102
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA T7 promoter sequence

<400>  102
guauaauacg acucacuaua ggga                                              24


