               SEQUENCE LISTING

<110> Antibody Innovations Ltd

<120> Polypeptides and Methods of Use

<130> J15464

<150> GB1905100.2
<151> 2019-04-10

<160> 24

<170> BiSSAP 1.3.6

<210> 1
<211> 1247
<212> DNA
<213> Artificial Sequence


<220> 
<223> nanoLuc designated Alemsnluc

<400> 1
atggcgcagg tgcagctgca ggaaagcggc ccgggcctgg tgcgcccgag ccagaccctg      60

agcctgacct gcaccgtgag cggctttacc tttaccgatt tttatatgaa ctgggtgcgc     120

cagccgccgg gccgcggcct ggaatggatt ggctttattc gcgataaagc gaaaggctat     180

accaccgaat ataacccgag cgtgaaaggc cgcgtgacca tgctggtgga taccagcaaa     240

aaccagttta gcctgcgcct gagcagcgtg accgcggcgg ataccgcggt gtattattgc     300

gcgcgcgaag gccataccgc ggcgccgttt gattattggg gccagggcag cctggtgacc     360

gtgagcagcg gtggcggggg atccgtgttt accctggaag attttgtggg cgattggcgc     420

cagaccgcgg gctataacct ggatcaggtg ctggaacagg gcggcgtgag cagcctgttt     480

cagaacctgg gcgtgagcgt gaccccgatt cagcgcattg tgctgagcgg cgaaaacggc     540

ctgaaaattg atattcatgt gattattccg tatgaaggcc tgagcggcga tcagatgggc     600

cagattgaaa aaatttttaa agtggtgtat ccggtggatg atcatcattt taaagtgatt     660

ctgcattatg gcaccctggt gattgatggc gtgaccccga acatgattga ttattttggc     720

cgcccgtatg aaggcattgc ggtgtttgat ggcaaaaaaa ttaccgtgac cggcaccctg     780

tggaacggca acaaaattat tgatgaacgc ctgattaacc cggatggcag cctgctgttt     840

cgcgtgacca ttaacggcgt gaccggctgg cgcctgtgcg aacgcattct ggcgggaggt     900

ggcggatccg atattcagat gacccagagc ccgagcagcc tgagcgcgag cgtgggcgat     960

cgcgtgacca ttacctgcaa agcgagccag aacattgata aatatctgaa ctggtatcag    1020

cagaaaccgg gcaaagcgcc gaaactgctg atttataaca ccaacaacct gcagaccggc    1080

gtgccgagcc gctttagcgg cagcggcagc ggcaccgatt ttacctttac cattagcagc    1140

ctgcagccgg aagatattgc gacctattat tgcctgcagc atattagccg cccgcgcacc    1200

tttggccagg gcaccaaagt ggaaattaaa cgcgcggatg cggccgc                  1247


<210> 2
<211> 416
<212> PRT
<213> Artificial Sequence


<220> 
<223> nanoLuc designated Alemsnluc

<400> 2
Met Ala Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Arg Pro 
1               5                   10                  15      
Ser Gln Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Phe Thr Phe Thr 
            20                  25                  30          
Asp Phe Tyr Met Asn Trp Val Arg Gln Pro Pro Gly Arg Gly Leu Glu 
        35                  40                  45              
Trp Ile Gly Phe Ile Arg Asp Lys Ala Lys Gly Tyr Thr Thr Glu Tyr 
    50                  55                  60                  
Asn Pro Ser Val Lys Gly Arg Val Thr Met Leu Val Asp Thr Ser Lys 
65                  70                  75                  80  
Asn Gln Phe Ser Leu Arg Leu Ser Ser Val Thr Ala Ala Asp Thr Ala 
                85                  90                  95      
Val Tyr Tyr Cys Ala Arg Glu Gly His Thr Ala Ala Pro Phe Asp Tyr 
            100                 105                 110         
Trp Gly Gln Gly Ser Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
        115                 120                 125             
Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala Gly 
    130                 135                 140                 
Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu Phe 
145                 150                 155                 160 
Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu Ser 
                165                 170                 175     
Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr Glu 
            180                 185                 190         
Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys Val 
        195                 200                 205             
Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr Gly 
    210                 215                 220                 
Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe Gly 
225                 230                 235                 240 
Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr Val 
                245                 250                 255     
Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu Ile 
            260                 265                 270         
Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val Thr 
        275                 280                 285             
Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala Gly Gly Gly Gly Ser Asp 
    290                 295                 300                 
Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp 
305                 310                 315                 320 
Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Ile Asp Lys Tyr Leu 
                325                 330                 335     
Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr 
            340                 345                 350         
Asn Thr Asn Asn Leu Gln Thr Gly Val Pro Ser Arg Phe Ser Gly Ser 
        355                 360                 365             
Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu 
    370                 375                 380                 
Asp Ile Ala Thr Tyr Tyr Cys Leu Gln His Ile Ser Arg Pro Arg Thr 
385                 390                 395                 400 
Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Ala Asp Ala Ala Ala 
                405                 410                 415     



<210> 3
<211> 1773
<212> DNA
<213> Artificial Sequence


<220> 
<223> nanoluc designated Alemdnluc

<400> 3
atggcgcagg tgcagctgca ggaaagcggc ccgggcctgg tgcgcccgag ccagaccctg      60

agcctgacct gcaccgtgag cggctttacc tttaccgatt tttatatgaa ctgggtgcgc     120

cagccgccgg gccgcggcct ggaatggatt ggctttattc gcgataaagc gaaaggctat     180

accaccgaat ataacccgag cgtgaaaggc cgcgtgacca tgctggtgga taccagcaaa     240

aaccagttta gcctgcgcct gagcagcgtg accgcggcgg ataccgcggt gtattattgc     300

gcgcgcgaag gccataccgc ggcgccgttt gattattggg gccagggcag cctggtgacc     360

gtgagcagcg gtggcggggg atccgtcttc acactcgaag atttcgttgg ggactggcga     420

cagacagccg gctacaacct ggaccaagtc cttgaacagg gaggtgtgtc cagtttgttt     480

cagaatctcg gggtgtccgt aactccgatc caaaggattg tcctgagcgg tgaaaatggg     540

ctgaagatcg acatccatgt catcatcccg tatgaaggtc tgagcggcga ccaaatgggc     600

cagatcgaaa aaatttttaa agtggtgtac cctgtggatg atcatcactt taaggtgatc     660

ctgcactatg gcacactggt aatcgacggg gttacgccga acatgatcga ctatttcgga     720

cgggcgtatg aaagcatcgc cctgttcgac ggcaaaaaga tcactgtaac agggaccctg     780

tggaacggca acaaaattat cgacgaacgc ctgatcaacc ccgacgggtc cctgctgttc     840

cgagtaacca tcaacggaat gacccgctgg cggctgtgcg aacgcattct ggcgtctggt     900

tcaggaagtg tgtttaccct ggaagatttt gtgggcgatt ggcgccagac cgcgggctat     960

aacctggatc aggtgctgga acagggcggc gtgagcagcc tgtttcagaa cctgggcgtg    1020

agcgtgaccc cgattcagcg cattgtgctg agcggcgaaa acggcctgaa aattgatatt    1080

catgtgatta ttccgtatga aggcctgagc ggcgatcaga tgggccagat tgaaaaaatt    1140

tttaaagtgg tgtatccggt ggatgatcat cattttaaag tgattctgca ttatggcacc    1200

ctggtgattg atggcgtgac cccgaacatg attgattatt ttggccgccc gtatgaaggc    1260

attgcggtgt ttgatggcaa aaaaattacc gtgaccggca ccctgtggaa cggcaacaaa    1320

attattgatg aacgcctgat taacccggat ggcagcctgc tgtttcgcgt gaccattaac    1380

ggcgtgaccg gctggcgcct gtgtgagcgt atacttgcag gcggcggcgg atccgatatt    1440

cagatgaccc agagcccgag cagcctgagc gcgagcgtgg gcgatcgcgt gaccattacc    1500

tgcaaagcga gccagaacat tgataaatat ctgaactggt atcagcagaa accgggcaaa    1560

gcgccgaaac tgctgattta taacaccaac aacctgcaga ccggcgtgcc gagccgcttt    1620

agcggcagcg gcagcggcac cgattttacc tttaccatta gcagcctgca gccggaagat    1680

attgcgacct attattgcct gcagcatatt agccgcccgc gcacctttgg ccagggcacc    1740

aaagtggaaa ttaaacgcgc ggatgcggcc gca                                 1773


<210> 4
<211> 591
<212> PRT
<213> Artificial Sequence


<220> 
<223> nanoluc desginated Alemdnluc

<400> 4
Met Ala Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Arg Pro 
1               5                   10                  15      
Ser Gln Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Phe Thr Phe Thr 
            20                  25                  30          
Asp Phe Tyr Met Asn Trp Val Arg Gln Pro Pro Gly Arg Gly Leu Glu 
        35                  40                  45              
Trp Ile Gly Phe Ile Arg Asp Lys Ala Lys Gly Tyr Thr Thr Glu Tyr 
    50                  55                  60                  
Asn Pro Ser Val Lys Gly Arg Val Thr Met Leu Val Asp Thr Ser Lys 
65                  70                  75                  80  
Asn Gln Phe Ser Leu Arg Leu Ser Ser Val Thr Ala Ala Asp Thr Ala 
                85                  90                  95      
Val Tyr Tyr Cys Ala Arg Glu Gly His Thr Ala Ala Pro Phe Asp Tyr 
            100                 105                 110         
Trp Gly Gln Gly Ser Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
        115                 120                 125             
Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala Gly 
    130                 135                 140                 
Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu Phe 
145                 150                 155                 160 
Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu Ser 
                165                 170                 175     
Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr Glu 
            180                 185                 190         
Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys Val 
        195                 200                 205             
Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr Gly 
    210                 215                 220                 
Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe Gly 
225                 230                 235                 240 
Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr Val 
                245                 250                 255     
Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu Ile 
            260                 265                 270         
Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val Thr 
        275                 280                 285             
Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala Ser Gly Ser Gly Ser Val 
    290                 295                 300                 
Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala Gly Tyr 
305                 310                 315                 320 
Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu Phe Gln 
                325                 330                 335     
Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu Ser Gly 
            340                 345                 350         
Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr Glu Gly 
        355                 360                 365             
Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys Val Val 
    370                 375                 380                 
Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr Gly Thr 
385                 390                 395                 400 
Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe Gly Arg 
                405                 410                 415     
Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr Val Thr 
            420                 425                 430         
Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu Ile Asn 
        435                 440                 445             
Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val Thr Gly 
    450                 455                 460                 
Trp Arg Leu Cys Glu Arg Ile Leu Ala Gly Gly Gly Gly Ser Asp Ile 
465                 470                 475                 480 
Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg 
                485                 490                 495     
Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Ile Asp Lys Tyr Leu Asn 
            500                 505                 510         
Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Asn 
        515                 520                 525             
Thr Asn Asn Leu Gln Thr Gly Val Pro Ser Arg Phe Ser Gly Ser Gly 
    530                 535                 540                 
Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp 
545                 550                 555                 560 
Ile Ala Thr Tyr Tyr Cys Leu Gln His Ile Ser Arg Pro Arg Thr Phe 
                565                 570                 575     
Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Ala Asp Ala Ala Ala 
            580                 585                 590     

<210> 5
<211> 1056
<212> DNA
<213> Artificial Sequence


<220> 
<223> Dual Tandem Nanoluciferase domain

<400> 5
ggatccgtct tcacactcga agatttcgtt ggggactggc gacagacagc cggctacaac      60

ctggaccaag tccttgaaca gggaggtgtg tccagtttgt ttcagaatct cggggtgtcc     120

gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg ggctgaagat cgacatccat     180

gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg gccagatcga aaaaattttt     240

aaagtggtgt accctgtgga tgatcatcac tttaaggtga tcctgcacta tggcacactg     300

gtaatcgacg gggttacgcc gaacatgatc gactatttcg gacgggcgta tgaaagcatc     360

gccctgttcg acggcaaaaa gatcactgta acagggaccc tgtggaacgg caacaaaatt     420

atcgacgaac gcctgatcaa ccccgacggg tccctgctgt tccgagtaac catcaacgga     480

atgacccgct ggcggctgtg cgaacgcatt ctggcgtctg gttcaggaag tgtgtttacc     540

ctggaagatt ttgtgggcga ttggcgccag accgcgggct ataacctgga tcaggtgctg     600

gaacagggcg gcgtgagcag cctgtttcag aacctgggcg tgagcgtgac cccgattcag     660

cgcattgtgc tgagcggcga aaacggcctg aaaattgata ttcatgtgat tattccgtat     720

gaaggcctga gcggcgatca gatgggccag attgaaaaaa tttttaaagt ggtgtatccg     780

gtggatgatc atcattttaa agtgattctg cattatggca ccctggtgat tgatggcgtg     840

accccgaaca tgattgatta ttttggccgc ccgtatgaag gcattgcggt gtttgatggc     900

aaaaaaatta ccgtgaccgg caccctgtgg aacggcaaca aaattattga tgaacgcctg     960

attaacccgg atggcagcct gctgtttcgc gtgaccatta acggcgtgac cggctggcgc    1020

ctgtgtgagc gtatacttgc aggcggcggc ggatcc                              1056


<210> 6
<211> 352
<212> PRT
<213> Artificial Sequence


<220> 
<223> Dual Tandem Nanoluciferase domain

<400> 6
Gly Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr 
1               5                   10                  15      
Ala Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser 
            20                  25                  30          
Leu Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val 
        35                  40                  45              
Leu Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro 
    50                  55                  60                  
Tyr Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe 
65                  70                  75                  80  
Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His 
                85                  90                  95      
Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr 
            100                 105                 110         
Phe Gly Arg Ala Tyr Glu Ser Ile Ala Leu Phe Asp Gly Lys Lys Ile 
        115                 120                 125             
Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg 
    130                 135                 140                 
Leu Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly 
145                 150                 155                 160 
Met Thr Arg Trp Arg Leu Cys Glu Arg Ile Leu Ala Ser Gly Ser Gly 
                165                 170                 175     
Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
            180                 185                 190         
Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
        195                 200                 205             
Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
    210                 215                 220                 
Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
225                 230                 235                 240 
Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
                245                 250                 255     
Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
            260                 265                 270         
Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
        275                 280                 285             
Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
    290                 295                 300                 
Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
305                 310                 315                 320 
Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
                325                 330                 335     
Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala Gly Gly Gly Gly Ser 
            340                 345                 350         



<210> 7
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Linker

<400> 7
Ala Ser Thr Gly Ser 
1               5   

<210> 8
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Linker

<400> 8
Gly Gly Gly Gly Ser 
1               5   

<210> 9
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Linker

<400> 9
Ser Gly Ser Gly Ser 
1               5   

<210> 10
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Linker

<400> 10
Ala Thr Ser Gly Ser 
1               5   

<210> 11
<211> 724
<212> DNA
<213> Artificial Sequence


<220> 
<223> pK Adali scFv

<400> 11
ccatggcgga agtgcagctg gtggaaagcg gcggcggcct ggtgcagccg ggccgcagcc      60

tgcgcctgag ctgcgcggcg agcggcttta cctttgatga ttatgcgatg cattgggtgc     120

gccaggcgcc gggcaaaggc ctggaatggg tgagcgcgat tacctggaac agcggccata     180

ttgattatgc ggatagcgtg gaaggccgct ttaccattag ccgcgataac gcgaaaaaca     240

gcctgtatct gcagatgaac agcctgcgcg cggaagatac cgcggtgtat tattgcgcga     300

aagtgagcta tctgagcacc gcgagcagcc tggattattg gggccagggc accctggtga     360

ccgtgagcag cgcgagcacc ggatccgata ttcagatgac ccagagcccg agcagcctga     420

gcgcgagcgt gggcgatcgc gtgaccatta cctgccgcgc gagccagggc attcgcaact     480

atctggcgtg gtatcagcag aaaccgggca aagcgccgaa actgctgatt tatgcggcga     540

gcaccctgca gagcggcgtg ccgagccgct ttagcggcag cggcagcggc accgatttta     600

ccctgaccat tagcagcctg cagccggaag atgtggcgac ctattattgc cagcgctata     660

accgcgcgcc gtataccttt ggccagggca ccaaagtgga aattaaacgc gcggatgcgg     720

ccgc                                                                  724


<210> 12
<211> 715
<212> DNA
<213> Artificial Sequence


<220> 
<223> pK Inflixi scFv

<400> 12
ccatggcgga agtgaaactg gaagaaagcg gcggcggcct ggtgcagccg ggcggcagca      60

tgaaactgag ctgcgtggcg agcggcttta tttttagcaa ccattggatg aactgggtgc     120

gccagagccc ggaaaaaggc ctggaatggg tggcggaaat tcgcagcaaa agcattaaca     180

gcgcgaccca ttatgcggaa agcgtgaaag gccgctttac cattagccgc gatgatagca     240

aaagcgcggt gtatctgcag atgaccgatc tgcgcaccga agataccggc gtgtattatt     300

gcagccgcaa ctattatggc agcacctatg attattgggg ccagggcacc accctgaccg     360

tgagcagcgc gagcaccgga tccgatattc tgctgaccca gagcccggcg attctgagcg     420

tgagcccggg cgaacgcgtg agctttagct gccgcgcgag ccagtttgtg ggcagcagca     480

ttcattggta tcagcagcgc accaacggca gcccgcgcct gctgattaaa tatgcgagcg     540

aaagcatgag cggcattccg agccgcttta gcggcagcgg cagcggcacc gattttaccc     600

tgagcattaa caccgtggaa agcgaagata ttgcggatta ttattgccag cagagccata     660

gctggccgtt tacctttggc agcggcacca acctggaagt gaaacgcgcg gccgc          715


<210> 13
<211> 742
<212> DNA
<213> Artificial Sequence


<220> 
<223> pK Goli scFv

<400> 13
ccatggcaca ggtgcagctg gtggaaagcg gcggcggcgt ggtgcagccg ggccgcagcc      60

tgcgcctgag ctgcgcggcg agcggcttta tttttagcag ctatgcgatg cattgggtgc     120

gccaggcgcc gggcaacggc ctggaatggg tggcgtttat gagctatgat ggcagcaaca     180

aaaaatatgc ggatagcgtg aaaggccgct ttaccattag ccgcgataac agcaaaaaca     240

ccctgtatct gcagatgaac agcctgcgcg cggaagatac cgcggtgtat tattgcgcgc     300

gcgatcgcgg cattgcggcg ggcggcaact attattatta tggcatggat gtgtggggcc     360

agggcaccac cgtgaccgtg agcagcgcga gcaccggatc cgaaattgtg ctgacccaga     420

gcccggcgac cctgagcctg agcccgggcg aacgcgcgac cctgagctgc cgcgcgagcc     480

agagcgtgta tagctatctg gcgtggtatc agcagaaacc gggccaggcg ccgcgcctgc     540

tgatttatga tgcgagcaac cgcgcgaccg gcattccggc gcgctttagc ggcagcggca     600

gcggcaccga ttttaccctg accattagca gcctggaacc ggaagatttt gcggtgtatt     660

attgccagca gcgcagcaac tggccgccgt ttacctttgg cccgggcacc aaagtggata     720

ttaaacgcac cgtggcggcc gc                                              742


<210> 14
<211> 715
<212> DNA
<213> Artificial Sequence


<220> 
<223> pK Rituxi scFv

<400> 14
ccatggcgca ggtgcagctg cagcagccgg gcgcggaact ggtgaaaccg ggcgcgagcg      60

tgaaaatgag ctgcaaagcg agcggctata cctttaccag ctataacatg cattgggtga     120

aacagacccc gggccgcggc ctggaatgga ttggcgcgat ttatccgggc aacggcgata     180

ccagctataa ccagaaattt aaaggcaaag cgaccctgac cgcggataaa agcagcagca     240

ccgcgtatat gcagctgagc agcctgacca gcgaagatag cgcggtgtat tattgcgcgc     300

gcagcaccta ttatggcggc gattggtatt ttaacgtgtg gggcgcgggc accaccgtga     360

ccgtgagcgc ggcgagcacc ggatcccaga ttgtgctgag ccagagcccg gcgattctga     420

gcgcgagccc gggcgaaaaa gtgaccatga cctgccgcgc gagcagcagc gtgagctata     480

ttcattggtt tcagcagaaa ccgggcagca gcccgaaacc gtggatttat gcgaccagca     540

acctggcgag cggcgtgccg gtgcgcttta gcggcagcgg cagcggcacc agctatagcc     600

tgaccattag ccgcgtggaa gcggaagatg cggcgaccta ttattgccag cagtggacca     660

gcaacccgcc gacctttggc ggcggcacca aactggaaat taaacgcgcg gccgc          715


<210> 15
<211> 721
<212> DNA
<213> Artificial Sequence


<220> 
<223> pKTrast scFv

<400> 15
ccatggccga ggttcagctg gtggagtctg gcggtggcct ggtgcagcca gggggctcac      60

tccgtttgtc ctgtgcagct tctggcttca acattaaaga cacctatata cactgggtgc     120

gtcaggcccc gggtaagggc ctggaatggg ttgcaaggat ttatcctacg aatggttatg     180

ctagatatgc cgatagcgtc aagggccgtt tcactataag cgcagacaca tccaaaaaca     240

cagcctacct gcagatgaac agcctgcgtg ctgaggacac tgccgtctat tattgttcta     300

gatggggagg ggacggcttc tatgctatgg actactgggg tcaaggaacc ctggtcaccg     360

tctcctcggg cggaggcgga tccgatatcc agatgaccca gtccccgagc tccctgtccg     420

cctctgtggg cgatagggtc accatcacct gccgtgccag tcaggatgtg aatactgctg     480

tagcctggta tcaacagaaa ccaggaaaag ctccgaaact actgatttac tcggcatcct     540

tcctctactc tggagtcctt tctcgcttct ctgggtccag atctgggacg gatttcactc     600

tgaccatcag cagtctgcag ccggaggact tcgcaactta ttactgtcag caacattata     660

ctactcctcc cacgttcgga cagggtacca aggtggagat caaacgcgcg gatgcggccg     720

c                                                                     721


<210> 16
<211> 730
<212> DNA
<213> Artificial Sequence


<220> 
<223> pK Emici IXa scFv

<400> 16
ccatggccca ggtgcagctg gtggaaagcg gcggcggcct ggtgcagccg ggcggcagcc      60

tgcgcctgag ctgcgcggcg agcggcttta cctttagcta ttatgatatt cagtgggtgc     120

gccaggcgcc gggcaaaggc ctggaatggg tgagcagcat tagcccgagc ggccagagca     180

cctattatcg ccgcgaagtg aaaggccgct ttaccattag ccgcgataac agcaaaaaca     240

ccctgtatct gcagatgaac agcctgcgcg cggaagatac cgcggtgtat tattgcgcgc     300

gccgcaccgg ccgcgaatat ggcggcggct ggtattttga ttattggggc cagggcaccc     360

tggtgaccgt gtctagtgca agtactggat ccgatattca gatgacccag agcccgagca     420

gcctgagcgc gagcgtgggc gatcgcgtga ccattacctg caaagcgagc cgcaacattg     480

aacgccagct ggcgtggtat cagcagaaac cgggccaggc gccggaactg ctgatttatc     540

aggcgagccg caaagaaagc ggcgtgccgg atcgctttag cggcagccgc tatggcaccg     600

attttaccct gaccattagc agcctgcagc cggaagatat tgcgacctat tattgccagc     660

agtatagcga tccgccgctg acctttggcg gcggcaccaa agtggaaatt aaacgcaccg     720

tggcggccgc                                                            730


<210> 17
<211> 718
<212> DNA
<213> Artificial Sequence


<220> 
<223> pK Emici X scFv

<400> 17
ccatggccca ggtgcagctg gtgcagagcg gcagcgaact gaaaaaaccg ggcgcgagcg      60

tgaaagtgag ctgcaaagcg agcggctata cctttaccga taacaacatg gattgggtgc     120

gccaggcgcc gggccagggc ctggaatgga tgggcgatat taacacccgc agcggcggca     180

gcatttataa cgaagaattt caggatcgcg tgattatgac cgtggataaa agcaccgata     240

ccgcgtatat ggaactgagc agcctgcgca gcgaagatac cgcgacctat cattgcgcgc     300

gccgcaaaag ctatggctat tatctggatg aatggggcga aggcaccctg gtgaccgtgt     360

ctagtgcaag tactggatcc gatattcaga tgacccagag cccgagcagc ctgagcgcga     420

gcgtgggcga tcgcgtgacc attacctgca aagcgagccg caacattgaa cgccagctgg     480

cgtggtatca gcagaaaccg ggccaggcgc cggaactgct gatttatcag gcgagccgca     540

aagaaagcgg cgtgccggat cgctttagcg gcagccgcta tggcaccgat tttaccctga     600

ccattagcag cctgcagccg gaagatattg cgacctatta ttgccagcag tatagcgatc     660

cgccgctgac ctttggcggc ggcaccaaag tggaaattaa acgcaccgtg gcggccgc       718


<210> 18
<211> 7136
<212> DNA
<213> Artificial Sequence


<220> 
<223> pK

<400> 18
tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat       60

cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga      120

gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt      180

tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga      240

taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt      300

tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa      360

tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc      420

agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt      480

ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg      540

ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag      600

taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc      660

gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac      720

cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga      780

tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca      840

cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc      900

cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc      960

ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc     1020

agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg     1080

gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga     1140

ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc     1200

agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg     1260

gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg     1320

gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg     1380

ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc     1440

cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc     1500

agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat     1560

gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg     1620

atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc     1680

acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc     1740

gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac     1800

accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac     1860

ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc     1920

agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt     1980

tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa     2040

gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg     2100

aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg     2160

gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag     2220

taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc     2280

caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag     2340

cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac     2400

cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatcta     2460

acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc     2520

cggctcgtat gttgtgtgga attgtgagcg gataacaatt tccctctaga aataattttg     2580

tttaacttta agaaggagat atacatatga aatacctgct gccgaccgct gctgctggtc     2640

tgctgctcct cgctgcccag ccggccatgg cggaagtgaa actggaagaa agcggcggcg     2700

gcctggtgca gccgggcggc agcatgaaac tgagctgcgt ggcgagcggc tttattttta     2760

gcaaccattg gatgaactgg gtgcgccaga gcccggaaaa aggcctggaa tgggtggcgg     2820

aaattcgcag caaaagcatt aacagcgcga cccattatgc ggaaagcgtg aaaggccgct     2880

ttaccattag ccgcgatgat agcaaaagcg cggtgtatct gcagatgacc gatctgcgca     2940

ccgaagatac cggcgtgtat tattgcagcc gcaactatta tggcagcacc tatgattatt     3000

ggggccaggg caccaccctg accgtgagca gcgcgagcac cggatccgtc ttcacactcg     3060

aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac     3120

agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga     3180

ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag     3240

gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg     3300

atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc     3360

cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa     3420

agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca     3480

accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt     3540

gcgaacgcat tctggcgtct ggttcaggaa gtgtgtttac cctggaagat tttgtgggcg     3600

attggcgcca gaccgcgggc tataacctgg atcaggtgct ggaacagggc ggcgtgagca     3660

gcctgtttca gaacctgggc gtgagcgtga ccccgattca gcgcattgtg ctgagcggcg     3720

aaaacggcct gaaaattgat attcatgtga ttattccgta tgaaggcctg agcggcgatc     3780

agatgggcca gattgaaaaa atttttaaag tggtgtatcc ggtggatgat catcatttta     3840

aagtgattct gcattatggc accctggtga ttgatggcgt gaccccgaac atgattgatt     3900

attttggccg cccgtatgaa ggcattgcgg tgtttgatgg caaaaaaatt accgtgaccg     3960

gcaccctgtg gaacggcaac aaaattattg atgaacgcct gattaacccg gatggcagcc     4020

tgctgtttcg cgtgaccatt aacggcgtga ccggctggcg cctgtgtgag cgtatacttg     4080

caggcggcgg cggatccgat attctgctga cccagagccc ggcgattctg agcgtgagcc     4140

cgggcgaacg cgtgagcttt agctgccgcg cgagccagtt tgtgggcagc agcattcatt     4200

ggtatcagca gcgcaccaac ggcagcccgc gcctgctgat taaatatgcg agcgaaagca     4260

tgagcggcat tccgagccgc tttagcggca gcggcagcgg caccgatttt accctgagca     4320

ttaacaccgt ggaaagcgaa gatattgcgg attattattg ccagcagagc catagctggc     4380

cgtttacctt tggcagcggc accaacctgg aagtgaaacg cgcggccgca tccgcacatc     4440

atcatcacca tcactaaagt gatggtgatg atgatgtgca gccggatcaa gcttgatccg     4500

gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta     4560

gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact     4620

atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg     4680

tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt     4740

tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc     4800

tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg     4860

gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg     4920

agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct     4980

cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg     5040

agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg     5100

tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc     5160

aaatatgtat ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact     5220

gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg     5280

aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga     5340

ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat     5400

caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca     5460

tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat     5520

caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt     5580

taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat     5640

caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg     5700

ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg     5760

gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg     5820

caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc     5880

gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat     5940

cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc     6000

tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa     6060

atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga     6120

tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg     6180

ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact     6240

ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac     6300

cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg     6360

gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg     6420

gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga     6480

acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc     6540

gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg     6600

agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc     6660

tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc     6720

agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt     6780

cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc     6840

gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc     6900

ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc     6960

tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg     7020

tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc     7080

ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgca         7136


<210> 19
<211> 7245
<212> DNA
<213> Artificial Sequence


<220> 
<223> pKamp

<400> 19
gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct       60

gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc      120

ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg      180

gccacgatgc gtccggcgta gaggatcgag atctaacgca attaatgtga gttagctcac      240

tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt      300

gagcggataa caatttccct ctagaaataa ttttgtttaa ctttaagaag gagatataca      360

tatgaaatac ctgctgccga ccgctgctgc tggtctgctg ctcctcgctg cccagccggc      420

catggcggaa gtgcagctgg tggaaagcgg cggcggcctg gtgcagccgg gccgcagcct      480

gcgcctgagc tgcgcggcga gcggctttac ctttgatgat tatgcgatgc attgggtgcg      540

ccaggcgccg ggcaaaggcc tggaatgggt gagcgcgatt acctggaaca gcggccatat      600

tgattatgcg gatagcgtgg aaggccgctt taccattagc cgcgataacg cgaaaaacag      660

cctgtatctg cagatgaaca gcctgcgcgc ggaagatacc gcggtgtatt attgcgcgaa      720

agtgagctat ctgagcaccg cgagcagcct ggattattgg ggccagggca ccctggtgac      780

cgtgagcagc gcgagcaccg gatccgtctt cacactcgaa gatttcgttg gggactggcg      840

acagacagcc ggctacaacc tggaccaagt ccttgaacag ggaggtgtgt ccagtttgtt      900

tcagaatctc ggggtgtccg taactccgat ccaaaggatt gtcctgagcg gtgaaaatgg      960

gctgaagatc gacatccatg tcatcatccc gtatgaaggt ctgagcggcg accaaatggg     1020

ccagatcgaa aaaattttta aggtggtgta ccctgtggat gatcatcact ttaaggtgat     1080

cctgcactat ggcacactgg taatcgacgg ggttacgccg aacatgatcg actatttcgg     1140

acggccgtat gaaggcatcg ccgtgttcga cggcaaaaag atcactgtaa cagggaccct     1200

gtggaacggc aacaaaatta tcgacgagcg cctgatcaac cccgacggct ccctgctgtt     1260

ccgagtaacc atcaacggag tgaccggctg gcggctgtgc gaacgcattc tggcgtctgg     1320

ttcaggaagt gtgtttaccc tggaagattt tgtgggcgat tggcgccaga ccgcgggcta     1380

taacctggat caggtgctgg aacagggcgg cgtgagcagc ctgtttcaga acctgggcgt     1440

gagcgtgacc ccgattcagc gcattgtgct gagcggcgaa aacggcctga aaattgatat     1500

tcatgtgatt attccgtatg aaggcctgag cggcgatcag atgggccaga ttgaaaaaat     1560

ttttaaagtg gtgtatccgg tggatgatca tcattttaaa gtgattctgc attatggcac     1620

cctggtgatt gatggcgtga ccccgaacat gattgattat tttggccgcc cgtatgaagg     1680

cattgcggtg tttgatggca aaaaaattac cgtgaccggc accctgtgga acggcaacaa     1740

aattattgat gaacgcctga ttaacccgga tggcagcctg ctgtttcgcg tgaccattaa     1800

cggcgtgacc ggctggcgcc tgtgtgagcg tatacttgca ggcggcggcg gatccgatat     1860

tcagatgacc cagagcccga gcagcctgag cgcgagcgtg ggcgatcgcg tgaccattac     1920

ctgccgcgcg agccagggca ttcgcaacta tctggcgtgg tatcagcaga aaccgggcaa     1980

agcgccgaaa ctgctgattt atgcggcgag caccctgcag agcggcgtgc cgagccgctt     2040

tagcggcagc ggcagcggca ccgattttac cctgaccatt agcagcctgc agccggaaga     2100

tgtggcgacc tattattgcc agcgctataa ccgcgcgccg tatacctttg gccagggcac     2160

caaagtggaa attaaacgcg cggatgcggc cgcactcgag caccaccacc accaccactg     2220

agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca     2280

ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg     2340

aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg     2400

ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct     2460

ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat     2520

cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt     2580

gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg     2640

acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac     2700

cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta     2760

aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca     2820

atttcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa     2880

atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat     2940

tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg     3000

gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa     3060

gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt     3120

gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt     3180

ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat     3240

tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg     3300

acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta     3360

cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat     3420

catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag     3480

cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa     3540

ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca     3600

ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc     3660

ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt     3720

atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc     3780

gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat     3840

atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt     3900

tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac     3960

cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc     4020

ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca     4080

actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta     4140

gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct     4200

ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg     4260

gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc     4320

acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta     4380

tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg     4440

gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt     4500

cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg     4560

cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg     4620

ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc     4680

gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg     4740

agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt     4800

tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc     4860

agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac     4920

acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt     4980

gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag     5040

gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc     5100

atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg     5160

ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt     5220

tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac     5280

tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat     5340

gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt     5400

aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca     5460

gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt     5520

tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg     5580

tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag     5640

gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc     5700

gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa     5760

taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat     5820

gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag     5880

tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct     5940

caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt     6000

gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg     6060

ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca     6120

gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc     6180

ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga     6240

tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatgtcc gcaccaacgc     6300

gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca     6360

gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca     6420

tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt     6480

tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg     6540

cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat     6600

gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa     6660

cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga     6720

tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc     6780

cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa     6840

tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca     6900

gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg     6960

ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca     7020

cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg     7080

gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa     7140

aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc     7200

attaggaagc agcccagtag taggttgagg ccgttgagca ccgcc                     7245


<210> 20
<211> 14
<212> PRT
<213> Artificial Sequence


<220> 
<223> Linker X-nluc1 and X-nluc2

<400> 20
Gly Ser Xaa Ser Gly Ser Gly Ser Xaa Gly Gly Glu Gly Ser 
1               5                   10                  

<210> 21
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Linker

<400> 21
Gly Gly Gly Gly Ser 
1               5   

<210> 22
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Linker

<400> 22
Ser Gly Ser Gly Ser 
1               5   

<210> 23
<211> 531
<212> DNA
<213> Artificial Sequence


<220> 
<223> Single Nanoluciferase domain

<400> 23
ggatccgtgt ttaccctgga agattttgtg ggcgattggc gccagaccgc gggctataac      60

ctggatcagg tgctggaaca gggcggcgtg agcagcctgt ttcagaacct gggcgtgagc     120

gtgaccccga ttcagcgcat tgtgctgagc ggcgaaaacg gcctgaaaat tgatattcat     180

gtgattattc cgtatgaagg cctgagcggc gatcagatgg gccagattga aaaaattttt     240

aaagtggtgt atccggtgga tgatcatcat tttaaagtga ttctgcatta tggcaccctg     300

gtgattgatg gcgtgacccc gaacatgatt gattattttg gccgcccgta tgaaggcatt     360

gcggtgtttg atggcaaaaa aattaccgtg accggcaccc tgtggaacgg caacaaaatt     420

attgatgaac gcctgattaa cccggatggc agcctgctgt ttcgcgtgac cattaacggc     480

gtgaccggct ggcgcctgtg cgaacgcatt ctggcgggcg gcggcggatc c              531


<210> 24
<211> 177
<212> PRT
<213> Artificial Sequence


<220> 
<223> Single Nanoluciferase Sequence

<400> 24
Gly Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr 
1               5                   10                  15      
Ala Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser 
            20                  25                  30          
Leu Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val 
        35                  40                  45              
Leu Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro 
    50                  55                  60                  
Tyr Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe 
65                  70                  75                  80  
Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His 
                85                  90                  95      
Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr 
            100                 105                 110         
Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile 
        115                 120                 125             
Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg 
    130                 135                 140                 
Leu Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly 
145                 150                 155                 160 
Val Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala Gly Gly Gly Gly 
                165                 170                 175     
Ser 
    

