                         SEQUENCE LISTING

<110>  Inflazome Limited
 
<120>  Inflammasome Inhibition

<130>  ASE/91532PCT

<140>  PCT/EP2020/060356
<141>  2020-04-11

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  1027
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Purified recombinant hNLRP3 (6His-SUMO-TEV-NLRP3 [125-1036])

<400>  1

Met Gly His His His His His His Gly Ser Leu Gln Asp Ser Glu Val 
1               5                   10                  15      


Asn Gln Glu Ala Lys Pro Glu Val Lys Pro Glu Val Lys Pro Glu Thr 
            20                  25                  30          


His Ile Asn Leu Lys Val Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys 
        35                  40                  45              


Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu Met Glu Ala Phe Ala Lys 
    50                  55                  60                  


Arg Gln Gly Lys Glu Met Asp Ser Leu Thr Phe Leu Tyr Asp Gly Ile 
65                  70                  75                  80  


Glu Ile Gln Ala Asp Gln Thr Pro Glu Asp Leu Asp Met Glu Asp Asn 
                85                  90                  95      


Asp Ile Ile Glu Ala His Arg Glu Gln Ile Gly Gly Glu Asn Leu Tyr 
            100                 105                 110         


Phe Gln Gly Ser Arg Ile Ser Ile Cys Lys Met Lys Lys Asp Tyr Arg 
        115                 120                 125             


Lys Lys Tyr Arg Lys Tyr Val Arg Ser Arg Phe Gln Cys Ile Glu Asp 
    130                 135                 140                 


Arg Asn Ala Arg Leu Gly Glu Ser Val Ser Leu Asn Lys Arg Tyr Thr 
145                 150                 155                 160 


Arg Leu Arg Leu Ile Lys Glu His Arg Ser Gln Gln Glu Arg Glu Gln 
                165                 170                 175     


Glu Leu Leu Ala Ile Gly Lys Thr Lys Thr Cys Glu Ser Pro Val Ser 
            180                 185                 190         


Pro Ile Lys Met Glu Leu Leu Phe Asp Pro Asp Asp Glu His Ser Glu 
        195                 200                 205             


Pro Val His Thr Val Val Phe Gln Gly Ala Ala Gly Ile Gly Lys Thr 
    210                 215                 220                 


Ile Leu Ala Arg Lys Met Met Leu Asp Trp Ala Ser Gly Thr Leu Tyr 
225                 230                 235                 240 


Gln Asp Arg Phe Asp Tyr Leu Phe Tyr Ile His Cys Arg Glu Val Ser 
                245                 250                 255     


Leu Val Thr Gln Arg Ser Leu Gly Asp Leu Ile Met Ser Cys Cys Pro 
            260                 265                 270         


Asp Pro Asn Pro Pro Ile His Lys Ile Val Arg Lys Pro Ser Arg Ile 
        275                 280                 285             


Leu Phe Leu Met Asp Gly Phe Asp Glu Leu Gln Gly Ala Phe Asp Glu 
    290                 295                 300                 


His Ile Gly Pro Leu Cys Thr Asp Trp Gln Lys Ala Glu Arg Gly Asp 
305                 310                 315                 320 


Ile Leu Leu Ser Ser Leu Ile Arg Lys Lys Leu Leu Pro Glu Ala Ser 
                325                 330                 335     


Leu Leu Ile Thr Thr Arg Pro Val Ala Leu Glu Lys Leu Gln His Leu 
            340                 345                 350         


Leu Asp His Pro Arg His Val Glu Ile Leu Gly Phe Ser Glu Ala Lys 
        355                 360                 365             


Arg Lys Glu Tyr Phe Phe Lys Tyr Phe Ser Asp Glu Ala Gln Ala Arg 
    370                 375                 380                 


Ala Ala Phe Ser Leu Ile Gln Glu Asn Glu Val Leu Phe Thr Met Cys 
385                 390                 395                 400 


Phe Ile Pro Leu Val Cys Trp Ile Val Cys Thr Gly Leu Lys Gln Gln 
                405                 410                 415     


Met Glu Ser Gly Lys Ser Leu Ala Gln Thr Ser Lys Thr Thr Thr Ala 
            420                 425                 430         


Val Tyr Val Phe Phe Leu Ser Ser Leu Leu Gln Pro Arg Gly Gly Ser 
        435                 440                 445             


Gln Glu His Gly Leu Cys Ala His Leu Trp Gly Leu Cys Ser Leu Ala 
    450                 455                 460                 


Ala Asp Gly Ile Trp Asn Gln Lys Ile Leu Phe Glu Glu Ser Asp Leu 
465                 470                 475                 480 


Arg Asn His Gly Leu Gln Lys Ala Asp Val Ser Ala Phe Leu Arg Met 
                485                 490                 495     


Asn Leu Phe Gln Lys Glu Val Asp Cys Glu Lys Phe Tyr Ser Phe Ile 
            500                 505                 510         


His Met Thr Phe Gln Glu Phe Phe Ala Ala Met Tyr Tyr Leu Leu Glu 
        515                 520                 525             


Glu Glu Lys Glu Gly Arg Thr Asn Val Pro Gly Ser Arg Leu Lys Leu 
    530                 535                 540                 


Pro Ser Arg Asp Val Thr Val Leu Leu Glu Asn Tyr Gly Lys Phe Glu 
545                 550                 555                 560 


Lys Gly Tyr Leu Ile Phe Val Val Arg Phe Leu Phe Gly Leu Val Asn 
                565                 570                 575     


Gln Glu Arg Thr Ser Tyr Leu Glu Lys Lys Leu Ser Cys Lys Ile Ser 
            580                 585                 590         


Gln Gln Ile Arg Leu Glu Leu Leu Lys Trp Ile Glu Val Lys Ala Lys 
        595                 600                 605             


Ala Lys Lys Leu Gln Ile Gln Pro Ser Gln Leu Glu Leu Phe Tyr Cys 
    610                 615                 620                 


Leu Tyr Glu Met Gln Glu Glu Asp Phe Val Gln Arg Ala Met Asp Tyr 
625                 630                 635                 640 


Phe Pro Lys Ile Glu Ile Asn Leu Ser Thr Arg Met Asp His Met Val 
                645                 650                 655     


Ser Ser Phe Cys Ile Glu Asn Cys His Arg Val Glu Ser Leu Ser Leu 
            660                 665                 670         


Gly Phe Leu His Asn Met Pro Lys Glu Glu Glu Glu Glu Glu Lys Glu 
        675                 680                 685             


Gly Arg His Leu Asp Met Val Gln Cys Val Leu Pro Ser Ser Ser His 
    690                 695                 700                 


Ala Ala Cys Ser His Gly Leu Val Asn Ser His Leu Thr Ser Ser Phe 
705                 710                 715                 720 


Cys Arg Gly Leu Phe Ser Val Leu Ser Thr Ser Gln Ser Leu Thr Glu 
                725                 730                 735     


Leu Asp Leu Ser Asp Asn Ser Leu Gly Asp Pro Gly Met Arg Val Leu 
            740                 745                 750         


Cys Glu Thr Leu Gln His Pro Gly Cys Asn Ile Arg Arg Leu Trp Leu 
        755                 760                 765             


Gly Arg Cys Gly Leu Ser His Glu Cys Cys Phe Asp Ile Ser Leu Val 
    770                 775                 780                 


Leu Ser Ser Asn Gln Lys Leu Val Glu Leu Asp Leu Ser Asp Asn Ala 
785                 790                 795                 800 


Leu Gly Asp Phe Gly Ile Arg Leu Leu Cys Val Gly Leu Lys His Leu 
                805                 810                 815     


Leu Cys Asn Leu Lys Lys Leu Trp Leu Val Ser Cys Cys Leu Thr Ser 
            820                 825                 830         


Ala Cys Cys Gln Asp Leu Ala Ser Val Leu Ser Thr Ser His Ser Leu 
        835                 840                 845             


Thr Arg Leu Tyr Val Gly Glu Asn Ala Leu Gly Asp Ser Gly Val Ala 
    850                 855                 860                 


Ile Leu Cys Glu Lys Ala Lys Asn Pro Gln Cys Asn Leu Gln Lys Leu 
865                 870                 875                 880 


Gly Leu Val Asn Ser Gly Leu Thr Ser Val Cys Cys Ser Ala Leu Ser 
                885                 890                 895     


Ser Val Leu Ser Thr Asn Gln Asn Leu Thr His Leu Tyr Leu Arg Gly 
            900                 905                 910         


Asn Thr Leu Gly Asp Lys Gly Ile Lys Leu Leu Cys Glu Gly Leu Leu 
        915                 920                 925             


His Pro Asp Cys Lys Leu Gln Val Leu Glu Leu Asp Asn Cys Asn Leu 
    930                 935                 940                 


Thr Ser His Cys Cys Trp Asp Leu Ser Thr Leu Leu Thr Ser Ser Gln 
945                 950                 955                 960 


Ser Leu Arg Lys Leu Ser Leu Gly Asn Asn Asp Leu Gly Asp Leu Gly 
                965                 970                 975     


Val Met Met Phe Cys Glu Val Leu Lys Gln Gln Ser Cys Leu Leu Gln 
            980                 985                 990         


Asn Leu Gly Leu Ser Glu Met Tyr  Phe Asn Tyr Glu Thr  Lys Ser Ala 
        995                 1000                 1005             


Leu Glu  Thr Leu Gln Glu Glu  Lys Pro Glu Leu Thr  Val Val Phe 
    1010                 1015                 1020             


Glu Pro  Ser Trp 
    1025         


<210>  2
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  6His-SUMO-TEV tag

<400>  2

Met Gly His His His His His His Gly Ser Leu Gln Asp Ser Glu Val 
1               5                   10                  15      


Asn Gln Glu Ala Lys Pro Glu Val Lys Pro Glu Val Lys Pro Glu Thr 
            20                  25                  30          


His Ile Asn Leu Lys Val Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys 
        35                  40                  45              


Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu Met Glu Ala Phe Ala Lys 
    50                  55                  60                  


Arg Gln Gly Lys Glu Met Asp Ser Leu Thr Phe Leu Tyr Asp Gly Ile 
65                  70                  75                  80  


Glu Ile Gln Ala Asp Gln Thr Pro Glu Asp Leu Asp Met Glu Asp Asn 
                85                  90                  95      


Asp Ile Ile Glu Ala His Arg Glu Gln Ile Gly Gly Glu Asn Leu Tyr 
            100                 105                 110         


Phe Gln Gly Ser 
        115     


<210>  3
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified hNLRP3 peptide

<400>  3

Thr Cys Glu Ser Pro Val Ser Pro Ile Lys 
1               5                   10  


