                         SEQUENCE LISTING

<110>  bluebird bio, Inc.
       Friedman, Kevin
       Alonzo, Eric Scott
 
<120>  MANUFACTURING ANTI-BCMA CAR T CELLS

<130>  BLUE-118.PC

<150>  US 62/830,004
<151>  2019-04-05

<150>  US 62/944,485
<151>  2019-12-06

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  493
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - synthesized anti-BMCA CAR

<400>  1

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Val Leu Thr Gln Ser Pro Pro Ser Leu 
            20                  25                  30          


Ala Met Ser Leu Gly Lys Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu 
        35                  40                  45              


Ser Val Thr Ile Leu Gly Ser His Leu Ile His Trp Tyr Gln Gln Lys 
    50                  55                  60                  


Pro Gly Gln Pro Pro Thr Leu Leu Ile Gln Leu Ala Ser Asn Val Gln 
65                  70                  75                  80  


Thr Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe 
                85                  90                  95      


Thr Leu Thr Ile Asp Pro Val Glu Glu Asp Asp Val Ala Val Tyr Tyr 
            100                 105                 110         


Cys Leu Gln Ser Arg Thr Ile Pro Arg Thr Phe Gly Gly Gly Thr Lys 
        115                 120                 125             


Leu Glu Ile Lys Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly 
    130                 135                 140                 


Glu Gly Ser Thr Lys Gly Gln Ile Gln Leu Val Gln Ser Gly Pro Glu 
145                 150                 155                 160 


Leu Lys Lys Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly 
                165                 170                 175     


Tyr Thr Phe Thr Asp Tyr Ser Ile Asn Trp Val Lys Arg Ala Pro Gly 
            180                 185                 190         


Lys Gly Leu Lys Trp Met Gly Trp Ile Asn Thr Glu Thr Arg Glu Pro 
        195                 200                 205             


Ala Tyr Ala Tyr Asp Phe Arg Gly Arg Phe Ala Phe Ser Leu Glu Thr 
    210                 215                 220                 


Ser Ala Ser Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys Tyr Glu Asp 
225                 230                 235                 240 


Thr Ala Thr Tyr Phe Cys Ala Leu Asp Tyr Ser Tyr Ala Met Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala Ala Ala Thr Thr 
            260                 265                 270         


Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln 
        275                 280                 285             


Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala 
    290                 295                 300                 


Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala 
305                 310                 315                 320 


Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr 
                325                 330                 335     


Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
            340                 345                 350         


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
        355                 360                 365             


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
    370                 375                 380                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
385                 390                 395                 400 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                405                 410                 415     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            420                 425                 430         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        435                 440                 445             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    450                 455                 460                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
465                 470                 475                 480 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490             


<210>  2
<211>  1485
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - synthesized anti-BMCA CAR

<400>  2
atggcactcc ccgtcaccgc ccttctcttg cccctcgccc tgctgctgca tgctgccagg       60

cccgacattg tgctcactca gtcacctccc agcctggcca tgagcctggg aaaaagggcc      120

accatctcct gtagagccag tgagtccgtc acaatcttgg ggagccatct tattcactgg      180

tatcagcaga agcccgggca gcctccaacc cttcttattc agctcgcgtc aaacgtccag      240

acgggtgtac ctgccagatt ttctggtagc gggtcccgca ctgattttac actgaccata      300

gatccagtgg aagaagacga tgtggccgtg tattattgtc tgcagagcag aacgattcct      360

cgcacatttg gtgggggtac taagctggag attaagggaa gcacgtccgg ctcagggaag      420

ccgggctccg gcgagggaag cacgaagggg caaattcagc tggtccagag cggacctgag      480

ctgaaaaaac ccggcgagac tgttaagatc agttgtaaag catctggcta taccttcacc      540

gactacagca taaattgggt gaaacgggcc cctggaaagg gcctcaaatg gatgggttgg      600

atcaataccg aaactaggga gcctgcttat gcatatgact tccgcgggag attcgccttt      660

tcactcgaga catctgcctc tactgcttac ctccaaataa acaacctcaa gtatgaagat      720

acagccactt acttttgcgc cctcgactat agttacgcca tggactactg gggacaggga      780

acctccgtta ccgtcagttc cgcggccgca accacaacac ctgctccaag gccccccaca      840

cccgctccaa ctatagccag ccaaccattg agcctcagac ctgaagcttg caggcccgca      900

gcaggaggcg ccgtccatac gcgaggcctg gacttcgcgt gtgatattta tatttgggcc      960

cctttggccg gaacatgtgg ggtgttgctt ctctcccttg tgatcactct gtattgtaag     1020

cgcgggagaa agaagctcct gtacatcttc aagcagcctt ttatgcgacc tgtgcaaacc     1080

actcaggaag aagatgggtg ttcatgccgc ttccccgagg aggaagaagg agggtgtgaa     1140

ctgagggtga aattttctag aagcgccgat gctcccgcat atcagcaggg tcagaatcag     1200

ctctacaatg aattgaatct cggcaggcga gaagagtacg atgttctgga caagagacgg     1260

ggcagggatc ccgagatggg gggaaagccc cggagaaaaa atcctcagga ggggttgtac     1320

aatgagctgc agaaggacaa gatggctgaa gcctatagcg agatcggaat gaaaggcgaa     1380

agacgcagag gcaaggggca tgacggtctg taccagggtc tctctacagc caccaaggac     1440

acttatgatg cgttgcatat gcaagccttg ccaccccgct aatga                     1485


