                         SEQUENCE LISTING

<110>  Case Western Reserve University
 
<120>  Method for detecting tumor-associated proteins in blood

<130>  28728-147

<150>  US 62/825,848
<151>  2019-03-29

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  1357
<212>  PRT
<213>  homo sapiens

<400>  1

Met Gly Ala Ala Gly Arg Gln Asp Phe Leu Phe Lys Ala Met Leu Thr 
1               5                   10                  15      


Ile Ser Trp Leu Thr Leu Thr Cys Phe Pro Gly Ala Thr Ser Thr Val 
            20                  25                  30          


Ala Ala Gly Cys Pro Asp Gln Ser Pro Glu Leu Gln Pro Trp Asn Pro 
        35                  40                  45              


Gly His Asp Gln Asp His His Val His Ile Gly Gln Gly Lys Thr Leu 
    50                  55                  60                  


Leu Leu Thr Ser Ser Ala Thr Val Tyr Ser Ile His Ile Ser Glu Gly 
65                  70                  75                  80  


Gly Lys Leu Val Ile Lys Asp His Asp Glu Pro Ile Val Leu Arg Thr 
                85                  90                  95      


Arg His Ile Leu Ile Asp Asn Gly Gly Glu Leu His Ala Gly Ser Ala 
            100                 105                 110         


Leu Cys Pro Phe Gln Gly Asn Phe Thr Ile Ile Leu Tyr Gly Arg Ala 
        115                 120                 125             


Asp Glu Gly Ile Gln Pro Asp Pro Tyr Tyr Gly Leu Lys Tyr Ile Gly 
    130                 135                 140                 


Val Gly Lys Gly Gly Ala Leu Glu Leu His Gly Gln Lys Lys Leu Ser 
145                 150                 155                 160 


Trp Thr Phe Leu Asn Lys Thr Leu His Pro Gly Gly Met Ala Glu Gly 
                165                 170                 175     


Gly Tyr Phe Phe Glu Arg Ser Trp Gly His Arg Gly Val Ile Val His 
            180                 185                 190         


Val Ile Asp Pro Lys Ser Gly Thr Val Ile His Ser Arg Asp Phe Asp 
        195                 200                 205             


Thr Tyr Arg Ser Lys Lys Glu Ser Glu Arg Leu Val Gln Tyr Leu Asn 
    210                 215                 220                 


Ala Val Pro Asp Gly Arg Ile Leu Ser Val Ala Val Asn Asp Glu Gly 
225                 230                 235                 240 


Ser Arg Asn Leu Asp Asp Met Ala Arg Lys Ala Met Thr Lys Leu Gly 
                245                 250                 255     


Ser Lys His Phe Leu His Leu Gly Phe Arg His Pro Trp Ser Phe Leu 
            260                 265                 270         


Thr Val Lys Gly Asn Pro Ser Ser Ser Val Glu Asp His Ile Glu Tyr 
        275                 280                 285             


His Gly His Arg Gly Ser Ala Ala Ala Arg Val Phe Lys Leu Phe Gln 
    290                 295                 300                 


Thr Glu His Gly Glu Tyr Phe Asn Val Ser Leu Ser Ser Glu Trp Val 
305                 310                 315                 320 


Gln Asp Val Glu Trp Thr Glu Trp Phe Asp His Asp Lys Val Ser Gln 
                325                 330                 335     


Thr Lys Cys Phe Glu Lys Ile Ser Asp Leu Trp Lys Ala His Pro Gly 
            340                 345                 350         


Lys Ile Cys Asn Arg Pro Ile Asp Ile Gln Ala Thr Thr Met Asp Gly 
        355                 360                 365             


Val Asn Leu Ser Thr Glu Val Val Tyr Lys Lys Gly Gln Asp Tyr Arg 
    370                 375                 380                 


Phe Ala Cys Tyr Asp Arg Gly Arg Ala Cys Arg Ser Tyr Arg Val Arg 
385                 390                 395                 400 


Phe Leu Cys Gly Lys Pro Val Arg Pro Lys Leu Thr Val Thr Ile Asp 
                405                 410                 415     


Thr Asn Val Asn Ser Thr Ile Leu Asn Leu Glu Asp Asn Val Gln Ser 
            420                 425                 430         


Trp Lys Pro Gly Asp Thr Leu Val Ile Ala Ser Thr Asp Tyr Ser Met 
        435                 440                 445             


Tyr Gln Ala Glu Glu Phe Gln Val Leu Pro Cys Arg Ser Cys Ala Pro 
    450                 455                 460                 


Asn Gln Val Lys Val Ala Gly Lys Pro Met Tyr Leu His Ile Gly Glu 
465                 470                 475                 480 


Glu Ile Asp Gly Val Asp Met Arg Ala Glu Val Gly Leu Leu Ser Arg 
                485                 490                 495     


Asn Ile Ile Val Met Gly Glu Met Glu Asp Lys Cys Tyr Pro Tyr Arg 
            500                 505                 510         


Asn His Ile Cys Asn Phe Phe Asp Phe Asp Thr Phe Gly Gly His Ile 
        515                 520                 525             


Lys Phe Ala Leu Gly Phe Lys Ala Ala His Leu Glu Gly Thr Glu Leu 
    530                 535                 540                 


Lys His Met Gly Gln Gln Leu Val Gly Gln Tyr Pro Ile His Phe His 
545                 550                 555                 560 


Ala Gly Asp Val Asp Glu Arg Gly Gly Tyr Asp Pro Pro Thr Tyr Ile 
                565                 570                 575     


Arg Asp Leu Ser Ile His His Thr Phe Ser Arg Cys Val Thr Val His 
            580                 585                 590         


Gly Ser Asn Gly Leu Leu Ile Lys Asp Val Val Gly Tyr Asn Ser Leu 
        595                 600                 605             


Gly His Cys Phe Phe Thr Glu Asp Gly Pro Glu Glu Arg Asn Thr Phe 
    610                 615                 620                 


Asp His Cys Leu Gly Leu Leu Val Lys Ser Gly Thr Leu Leu Pro Ser 
625                 630                 635                 640 


Asp Arg Asp Ser Lys Met Cys Lys Met Ile Thr Glu Asp Ser Tyr Pro 
                645                 650                 655     


Gly Tyr Ile Pro Lys Pro Arg Gln Asp Cys Asn Ala Val Ser Thr Phe 
            660                 665                 670         


Trp Met Ala Asn Pro Asn Asn Asn Leu Ile Asn Cys Ala Ala Ala Gly 
        675                 680                 685             


Ser Glu Glu Thr Gly Phe Trp Phe Ile Phe His His Val Pro Thr Gly 
    690                 695                 700                 


Pro Ser Val Gly Met Tyr Ser Pro Gly Tyr Ser Glu His Ile Pro Leu 
705                 710                 715                 720 


Gly Lys Phe Tyr Asn Asn Arg Ala His Ser Asn Tyr Arg Ala Gly Met 
                725                 730                 735     


Ile Ile Asp Asn Gly Val Lys Thr Thr Glu Ala Ser Ala Lys Asp Lys 
            740                 745                 750         


Arg Pro Phe Leu Ser Ile Ile Ser Ala Arg Tyr Ser Pro His Gln Asp 
        755                 760                 765             


Ala Asp Pro Leu Lys Pro Arg Glu Pro Ala Ile Ile Arg His Phe Ile 
    770                 775                 780                 


Ala Tyr Lys Asn Gln Asp His Gly Ala Trp Leu Arg Gly Gly Asp Val 
785                 790                 795                 800 


Trp Leu Asp Ser Cys Arg Phe Ala Asp Asn Gly Ile Gly Leu Thr Leu 
                805                 810                 815     


Ala Ser Gly Gly Thr Phe Pro Tyr Asp Asp Gly Ser Lys Gln Glu Ile 
            820                 825                 830         


Lys Asn Ser Leu Phe Val Gly Glu Ser Gly Asn Val Gly Thr Glu Met 
        835                 840                 845             


Met Asp Asn Arg Ile Trp Gly Pro Gly Gly Leu Asp His Ser Gly Arg 
    850                 855                 860                 


Thr Leu Pro Ile Gly Gln Asn Phe Pro Ile Arg Gly Ile Gln Leu Tyr 
865                 870                 875                 880 


Asp Gly Pro Ile Asn Ile Gln Asn Cys Thr Phe Arg Lys Phe Val Ala 
                885                 890                 895     


Leu Glu Gly Arg His Thr Ala Ser Leu Ala Phe Arg Leu Asn Asn Ala 
            900                 905                 910         


Trp Gln Ser Cys Pro His Asn Asn Val Thr Gly Ile Ala Phe Glu Asp 
        915                 920                 925             


Val Pro Ile Thr Ser Arg Val Phe Phe Gly Glu Pro Gly Pro Trp Phe 
    930                 935                 940                 


Asn Gln Leu Asp Met Asp Gly Asp Lys Thr Ser Val Phe His Asp Val 
945                 950                 955                 960 


Asp Gly Ser Val Ser Glu Tyr Pro Gly Ser Tyr Leu Lys Thr Asn Asp 
                965                 970                 975     


Asn Trp Leu Val Arg His Pro Asp Cys Ile Asn Val Pro Asp Trp Arg 
            980                 985                 990         


Gly Ala Ile Cys Ser Gly Cys Tyr  Ala Gln Met Tyr Ile  Gln Ala Tyr 
        995                 1000                 1005             


Lys Thr  Ser Asn Leu Arg Met  Lys Ile Ile Lys Asn  Asp Phe Pro 
    1010                 1015                 1020             


Ser His  Pro Leu Tyr Leu Glu  Gly Ala Leu Thr Arg  Ser Thr His 
    1025                 1030                 1035             


Tyr Gln  Gln Tyr Gln Pro Val  Val Thr Leu Gln Lys  Gly Tyr Thr 
    1040                 1045                 1050             


Ile His  Trp Asp Gln Thr Ala  Pro Ala Glu Leu Ala  Ile Trp Leu 
    1055                 1060                 1065             


Ile Asn  Phe Asn Lys Gly Asp  Trp Ile Arg Val Gly  Leu Cys Tyr 
    1070                 1075                 1080             


Pro Arg  Gly Thr Thr Phe Ser  Ile Leu Ser Asp Val  His Asn Arg 
    1085                 1090                 1095             


Leu Leu  Lys Gln Thr Ser Lys  Thr Gly Val Phe Val  Arg Thr Leu 
    1100                 1105                 1110             


Gln Met  Asp Lys Val Glu Gln  Ser Tyr Pro Gly Arg  Ser His Tyr 
    1115                 1120                 1125             


Tyr Trp  Asp Glu Asp Ser Gly  Leu Leu Phe Leu Lys  Leu Lys Ala 
    1130                 1135                 1140             


Gln Asn  Glu Arg Glu Lys Phe  Ala Phe Cys Ser Met  Lys Gly Cys 
    1145                 1150                 1155             


Glu Arg  Ile Lys Ile Lys Ala  Leu Ile Pro Lys Asn  Ala Gly Val 
    1160                 1165                 1170             


Ser Asp  Cys Thr Ala Thr Ala  Tyr Pro Lys Phe Thr  Glu Arg Ala 
    1175                 1180                 1185             


Val Val  Asp Val Pro Met Pro  Lys Lys Leu Phe Gly  Ser Gln Leu 
    1190                 1195                 1200             


Lys Thr  Lys Asp His Phe Leu  Glu Val Lys Met Glu  Ser Ser Lys 
    1205                 1210                 1215             


Gln His  Phe Phe His Leu Trp  Asn Asp Phe Ala Tyr  Ile Glu Val 
    1220                 1225                 1230             


Asp Gly  Lys Lys Tyr Pro Ser  Ser Glu Asp Gly Ile  Gln Val Val 
    1235                 1240                 1245             


Val Ile  Asp Gly Asn Gln Gly  Arg Trp Ser His Thr  Ser Phe Arg 
    1250                 1255                 1260             


Asn Ser  Ile Leu Gln Gly Ile  Pro Trp Gln Leu Phe  Asn Tyr Val 
    1265                 1270                 1275             


Ala Thr  Ile Pro Asp Asn Ser  Ile Val Leu Met Ala  Ser Lys Gly 
    1280                 1285                 1290             


Arg Tyr  Val Ser Arg Gly Pro  Trp Thr Arg Val Leu  Glu Lys Leu 
    1295                 1300                 1305             


Gly Ala  Asp Arg Gly Leu Lys  Leu Lys Glu Gln Met  Ala Phe Val 
    1310                 1315                 1320             


Gly Phe  Lys Gly Ser Phe Arg  Pro Ile Trp Val Thr  Leu Asp Thr 
    1325                 1330                 1335             


Glu Asp  His Lys Ala Lys Ile  Phe Gln Trp Pro Ile  Pro Trp Lys 
    1340                 1345                 1350             


Lys Lys  Lys Leu 
    1355         


<210>  2
<211>  204
<212>  PRT
<213>  homo sapiens

<400>  2

Gly Glu Lys Ile Ser Asp Leu Trp Lys Ala His Pro Gly Lys Ile Cys 
1               5                   10                  15      


Asn Arg Pro Ile Asp Ile Gln Ala Thr Thr Met Asp Gly Val Asn Leu 
            20                  25                  30          


Ser Thr Glu Val Val Tyr Lys Lys Gly Gln Asp Tyr Arg Phe Ala Cys 
        35                  40                  45              


Tyr Asp Arg Gly Arg Ala Cys Arg Ser Tyr Arg Val Arg Phe Leu Cys 
    50                  55                  60                  


Gly Lys Pro Val Arg Pro Lys Leu Thr Val Thr Ile Asp Thr Asn Val 
65                  70                  75                  80  


Asn Ser Thr Ile Leu Asn Leu Glu Asp Asn Val Gln Ser Trp Lys Pro 
                85                  90                  95      


Gly Asp Thr Leu Val Ile Ala Ser Thr Asp Tyr Ser Met Tyr Gln Ala 
            100                 105                 110         


Glu Glu Phe Gln Val Leu Pro Cys Arg Ser Cys Ala Pro Asn Gln Val 
        115                 120                 125             


Lys Val Ala Gly Lys Pro Met Tyr Leu His Ile Gly Glu Glu Ile Asp 
    130                 135                 140                 


Gly Val Asp Met Arg Ala Glu Val Gly Leu Leu Ser Arg Asn Ile Ile 
145                 150                 155                 160 


Val Met Gly Glu Met Glu Asp Lys Cys Tyr Pro Tyr Arg Asn His Ile 
                165                 170                 175     


Cys Asn Phe Phe Asp Phe Asp Thr Phe Gly Gly His Ile Lys Phe Ala 
            180                 185                 190         


Leu Gly Phe Lys Ala Ala His Leu Glu Gly Thr Glu 
        195                 200                 


<210>  3
<211>  123
<212>  PRT
<213>  homo sapiens

<400>  3

Thr Ser Thr Val Ala Ala Gly Cys Pro Asp Gln Ser Pro Glu Leu Gln 
1               5                   10                  15      


Pro Trp Asn Pro Gly His Asp Gln Asp His His Val His Ile Gly Gln 
            20                  25                  30          


Gly Lys Thr Leu Leu Leu Thr Ser Ser Ala Thr Val Tyr Ser Ile His 
        35                  40                  45              


Ile Ser Glu Gly Gly Lys Leu Val Ile Lys Asp His Asp Glu Pro Ile 
    50                  55                  60                  


Val Leu Arg Thr Arg His Ile Leu Ile Asp Asn Gly Gly Glu Leu His 
65                  70                  75                  80  


Ala Gly Ser Ala Leu Cys Pro Phe Gln Gly Asn Phe Thr Ile Ile Leu 
                85                  90                  95      


Tyr Gly Arg Ala Asp Glu Gly Ile Gln Pro Asp Pro Tyr Tyr Gly Leu 
            100                 105                 110         


Lys Tyr Ile Gly Val Gly Lys Gly Gly Ala Leu 
        115                 120             


<210>  4
<211>  31
<212>  PRT
<213>  homo sapiens

<400>  4

Asp Arg Phe Asp Thr Tyr Arg Ser Lys Lys Glu Ser Glu Arg Leu Val 
1               5                   10                  15      


Gln Tyr Leu Asn Ala Val Pro Asp Gly Arg Ile Leu Ser Val Ala 
            20                  25                  30      


