SEQUENCE LISTING
<110> CSIR

<120> Cloning and expression of in-vivo refolded antibody fragment

<140> 2019_______

<141> --/--/2019

<150>  

<151>  

<160>  



<210> 1

<211> 721

<212> DNA

<213> Artificial sequence 

<220>

<223> DNA 

<400> 1

gagctcatat ggaagttcag ctggttgaaa gcggtggtgg tctggttcag cctggtggta   60

gcctgcgtct gagctgtgca gcaagcggtt atgattttac ccattatggt atgaattggg  120

ttcgtcaggc accgggtaaa ggtctggaat gggttggttg gattaatacc tataccggtg  180

aaccgaccta tgcagcagat tttaaacgtc gttttacctt tagcctggat accagcaaaa  240

gcaccgcata tctgcagatg aatagcctgc gtgcagagga taccgcagtg tattattgtg  300

caaaatatcc gtattattac ggcaccagcc attggtattt cgatgtttgg ggtcagggca  360

ccctggttac cgttagcagc gcaagcacca aaggtccgag cgtttttccg ctggcaccga  420

gcagcaaaag taccagcggt ggcaccgcag cactgggttg tctggttaaa gattattttc  480

cggaaccggt taccgtgagc tggaatagcg gtgcactgac cagcggtgtt catacctttc  540

cggcagttct gcagagcagc ggtctgtata gcctgagcag cgttgttacc gttccgagca  600

gcagcctggg cacccagacc tatatttgta atgttaatca taaaccgagc aataccaaag  660

tggataaaaa agtggaaccg aaaagctgcg ataaaaccca tctgtaatag ctcgagccgc  720

g                                                                  721



<210> 2

<211> 231

<212> PRT

<213> Artificial sequence 

<220>

<223> Polypeptide sequence of heavy chain of Ranibizumab

<400> 2

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
		5 		    10		        15	
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Asp Phe Thr His Tyr 
	    20			25		     30	
Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
	  35		    40			45			
Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Ala Asp Phe 
    50			55		    60	
Lys Arg Arg Phe Thr Phe Ser Leu Asp Thr Ser Lys Ser Thr Ala Tyr
65		   70			75		    80 
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
		85		    90			 95			
Ala Lys Tyr Pro Tyr Tyr Tyr Gly Thr Ser His Trp Tyr Phe Asp Val 
	   100			05		    110
Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly 
	115		    120			125
Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 
    130			135		   140	
Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val	
145		   150		       155		   160
Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
		 165		    170			175		
Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
	   180			185		   190
Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val 
        195		   200			205	
Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys 
    210			215		    220	
Ser Cys Asp Lys Thr His Leu
225	  	   230	



<210> 3

<211> 669

<212> DNA

<213> Artificial sequence 

<220>

<223> DNA 

<400> 3

gagctccatg gatattcagc tgacccagag cccgagcagc ctgagcgcaa gcgttggtga   60

tcgtgttacc attacctgta gcgcaagcca ggatattagc aattatctga attggtatca  120

gcagaaaccg ggtaaagcac cgaaagtgct gatctatttt accagcagcc tgcatagcgg  180

tgttccgagc cgttttagcg gtagcggtag tggcaccgat tttaccctga ccattagcag  240

cctgcagccg gaagattttg caacctatta ttgtcagcag tatagcaccg ttccgtggac  300

ctttggtcag ggcaccaaag ttgaaattaa acgtaccgtt gcagcaccga gcgtttttat  360

ctttccgcct agtgatgaac agctgaaaag cggcaccgca agcgttgttt gtctgctgaa  420

taacttttat ccgcgtgaag caaaagttca gtggaaagtt gataatgcac tgcagagcgg  480

taatagccaa gaaagcgtta ccgaacagga tagcaaagat agcacctata gcctgagcag  540

caccctgacc ctgagcaaag cagattatga aaaacacaaa gtgtatgcct gcgaagttac  600

ccatcagggt ctgagcagtc cggttaccaa aagttttaat cgtggtgaat gctaatagaa  660

gcttggtac                                                          669




<210> 4

<211> 214

<212> PRT

<213> Artificial sequence 

<220>

<223> Polypeptide sequence of Light chain of Ranibizumab

<400> 4

Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
		5		    10		        15		
Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Gln Asp Ile Ser Asn Tyr
	    20			25		   30			 
Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Val Leu Ile 
	 35		    40		        45	
Tyr Phe Thr Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly
     50			55		   60		
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65		    70			75		     80	
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Thr Val Pro Trp
		 85		     90			 95	
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
	    100			105		    110		
Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly
 	115		    120			125
Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130			135		   140	
Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln
145		    150			 155		    160
Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser
		165		    170			175	
Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
	    180			185		    190
Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
	195		   200			205
Phe Asn Arg Gly Glu Cys
    210
