                         SEQUENCE LISTING

<110>  Novo Nordisk A/S
 
<120>  GLUCOSE SENSITIVE INSULIN DERIVATIVES

<130>  180121WO01

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  human insulin A-chain

<400>  1

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  2
<211>  30
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  human insulin B-chain

<400>  2

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210>  3
<211>  21
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  A-chain of human insulin analogue

<400>  3

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Gln 
            20      


<210>  4
<211>  21
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  A-chain of human insulin analogue

<400>  4

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Glu Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  5
<211>  21
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  A-chain of human insulin analogue

<400>  5

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Glu Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  6
<211>  22
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  A-chain of human insulin analogue

<400>  6

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn Lys 
            20          


<210>  7
<211>  23
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  A-chain of human insulin analogue

<400>  7

Lys Pro Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  8
<211>  31
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  A-chain of human insulin analogue with peptide spacer

<400>  8

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Gln Gly Glu Ser Gly Glu Ser Gly Glu Ser Lys 
            20                  25                  30      


<210>  9
<211>  40
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  A-chain of human insulin analogue with peptide spacer

<400>  9

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Gln Gly Glu Ser Gly Glu Ser Gly Glu Ser Gly Glu 
            20                  25                  30          


Ser Gly Glu Ser Gly Glu Ser Lys 
        35                  40  


<210>  10
<211>  58
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  A-chain of human insulin analogue with peptide spacer

<400>  10

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Gln Gly Glu Ser Gly Glu Ser Gly Glu Ser Gly Glu 
            20                  25                  30          


Ser Gly Glu Ser Gly Glu Ser Gly Glu Ser Gly Glu Ser Gly Glu Ser 
        35                  40                  45              


Gly Glu Ser Gly Glu Ser Gly Glu Ser Lys 
    50                  55              


<210>  11
<211>  29
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue

<400>  11

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys 
            20                  25                  


<210>  12
<211>  29
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue

<400>  12

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe His Tyr Thr Pro Lys 
            20                  25                  


<210>  13
<211>  28
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue

<400>  13

Lys Pro Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe His Tyr Pro Lys 
            20                  25              


<210>  14
<211>  28
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue

<400>  14

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe His Tyr Pro Lys 
            20                  25              


<210>  15
<211>  29
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue

<400>  15

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe His Tyr Pro Gly Lys 
            20                  25                  


<210>  16
<211>  27
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue

<400>  16

Asn Lys Pro Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val 
1               5                   10                  15      


Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys 
            20                  25          


<210>  17
<211>  27
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue

<400>  17

Gly Lys Pro Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val 
1               5                   10                  15      


Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys 
            20                  25          


<210>  18
<211>  30
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue

<400>  18

Gly Lys Pro Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu 
1               5                   10                  15      


Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys 
            20                  25                  30  


<210>  19
<211>  29
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue

<400>  19

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Arg 
            20                  25                  


<210>  20
<211>  29
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue

<400>  20

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Lys Gly Phe Phe Tyr Thr Pro Arg 
            20                  25                  


<210>  21
<211>  46
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue with peptide spacer

<400>  21

Lys Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10                  15      


Ser Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu 
            20                  25                  30          


Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys 
        35                  40                  45      


<210>  22
<211>  46
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue with peptide spacer

<400>  22

Lys Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10                  15      


Ser Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu 
            20                  25                  30          


Tyr Leu Val Cys Gly Glu Arg Gly Phe His Tyr Thr Pro Lys 
        35                  40                  45      


<210>  23
<211>  47
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue with peptide spacer

<400>  23

Gly Lys Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
1               5                   10                  15      


Gly Ser Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala 
            20                  25                  30          


Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys 
        35                  40                  45          


<210>  24
<211>  42
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue with peptide spacer

<400>  24

Gly Lys Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Phe Val Asn 
1               5                   10                  15      


Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys 
            20                  25                  30          


Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys 
        35                  40          


<210>  25
<211>  37
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue with peptide spacer

<400>  25

Gly Lys Pro Gly Gly Gly Gly Ser Phe Val Asn Gln His Leu Cys Gly 
1               5                   10                  15      


Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe 
            20                  25                  30          


Phe Tyr Thr Pro Lys 
        35          


<210>  26
<211>  33
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue with peptide spacer

<400>  26

Gly Lys Pro Gly Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val 
1               5                   10                  15      


Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro 
            20                  25                  30          


Lys 
    


<210>  27
<211>  49
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue with peptide spacer

<400>  27

Gly Lys Pro Arg Gly Phe Phe Tyr Thr Pro Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val 
            20                  25                  30          


Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro 
        35                  40                  45              


Lys 
    


<210>  28
<211>  53
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  B-chain of human insulin analogue with peptide spacer

<400>  28

Thr Tyr Phe Phe Gly Arg Lys Pro Asp Gly Gly Gly Gly Ser Gly Gly 
1               5                   10                  15      


Gly Gly Ser Gly Gly Gly Gly Ser Phe Val Asn Gln His Leu Cys Gly 
            20                  25                  30          


Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe 
        35                  40                  45              


Phe Tyr Thr Pro Lys 
    50              


<210>  29
<211>  10
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  peptide spacer

<400>  29

Gly Glu Ser Gly Glu Ser Gly Glu Ser Lys 
1               5                   10  


<210>  30
<211>  19
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  peptide spacer

<400>  30

Gly Glu Ser Gly Glu Ser Gly Glu Ser Gly Glu Ser Gly Glu Ser Gly 
1               5                   10                  15      


Glu Ser Lys 
            


<210>  31
<211>  37
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  peptide spacer

<400>  31

Gly Glu Ser Gly Glu Ser Gly Glu Ser Gly Glu Ser Gly Glu Ser Gly 
1               5                   10                  15      


Glu Ser Gly Glu Ser Gly Glu Ser Gly Glu Ser Gly Glu Ser Gly Glu 
            20                  25                  30          


Ser Gly Glu Ser Lys 
        35          


<210>  32
<211>  4
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  peptide spacer

<400>  32

Gly Lys Pro Gly 
1               


<210>  33
<211>  8
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  peptide spacer

<400>  33

Gly Lys Pro Gly Gly Gly Gly Ser 
1               5               


<210>  34
<211>  13
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  peptide spacer

<400>  34

Gly Lys Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10              


<210>  35
<211>  18
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  peptide spacer

<400>  35

Gly Lys Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
1               5                   10                  15      


Gly Ser 
        


<210>  36
<211>  17
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  peptide spacer

<400>  36

Lys Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10                  15      


Ser 
    


<210>  37
<211>  20
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  peptide spacer

<400>  37

Gly Lys Pro Arg Gly Phe Phe Tyr Thr Pro Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  38
<211>  24
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  peptide spacer

<400>  38

Thr Tyr Phe Phe Gly Arg Lys Pro Asp Gly Gly Gly Gly Ser Gly Gly 
1               5                   10                  15      


Gly Gly Ser Gly Gly Gly Gly Ser 
            20                  


