                         SEQUENCE LISTING

<110>  Lonza Ltd
 
<120>  PROTEIN PRODUCTION IN MUT-METHYLOTROPHIC YEAST

<130>  LO009P1

<160>  49    

<170>  PatentIn version 3.5

<210>  1
<211>  663
<212>  PRT
<213>  Komagataella phaffii

<400>  1

Met Ala Ile Pro Glu Glu Phe Asp Ile Leu Val Leu Gly Gly Gly Ser 
1               5                   10                  15      


Ser Gly Ser Cys Ile Ala Gly Arg Leu Ala Asn Leu Asp His Ser Leu 
            20                  25                  30          


Lys Val Gly Leu Ile Glu Ala Gly Glu Asn Asn Leu Asn Asn Pro Trp 
        35                  40                  45              


Val Tyr Leu Pro Gly Ile Tyr Pro Arg Asn Met Lys Leu Asp Ser Lys 
    50                  55                  60                  


Thr Ala Ser Phe Tyr Thr Ser Asn Pro Ser Pro His Leu Asn Gly Arg 
65                  70                  75                  80  


Arg Ala Ile Val Pro Cys Ala Asn Ile Leu Gly Gly Gly Ser Ser Ile 
                85                  90                  95      


Asn Phe Met Met Tyr Thr Arg Gly Ser Ala Ser Asp Tyr Asp Asp Phe 
            100                 105                 110         


Glu Ala Glu Gly Trp Lys Thr Lys Asp Leu Leu Pro Leu Met Lys Lys 
        115                 120                 125             


Thr Glu Thr Tyr Gln Arg Ala Cys Asn Asn Pro Glu Ile His Gly Phe 
    130                 135                 140                 


Glu Gly Pro Ile Lys Val Ser Phe Gly Asn Tyr Thr Tyr Pro Val Cys 
145                 150                 155                 160 


Gln Asp Phe Leu Arg Ala Thr Glu Ser Gln Gly Ile Pro Tyr Val Asp 
                165                 170                 175     


Asp Leu Glu Asp Leu Val Thr Ala His Gly Ala Glu His Trp Leu Lys 
            180                 185                 190         


Trp Ile Asn Arg Asp Thr Gly Arg Arg Ser Asp Ser Ala His Ala Phe 
        195                 200                 205             


Val His Ser Thr Met Arg Asn His Asp Asn Leu Tyr Leu Ile Cys Asn 
    210                 215                 220                 


Thr Lys Val Asp Lys Ile Ile Val Glu Asp Gly Arg Ala Ala Ala Val 
225                 230                 235                 240 


Arg Thr Val Pro Ser Lys Pro Leu Asn Ala Lys Lys Pro Thr His Lys 
                245                 250                 255     


Val Tyr Arg Ala Arg Lys Gln Ile Val Leu Ser Cys Gly Thr Ile Ser 
            260                 265                 270         


Ser Pro Leu Val Leu Gln Arg Ser Gly Phe Gly Asp Pro Ile Lys Leu 
        275                 280                 285             


Arg Ala Ala Gly Val Lys Pro Leu Val Asn Leu Pro Gly Val Gly Arg 
    290                 295                 300                 


Asn Phe Gln Asp His Tyr Cys Phe Phe Ser Pro Tyr Arg Ile Lys Pro 
305                 310                 315                 320 


Gln Tyr Glu Ser Phe Asp Asp Phe Val Arg Gly Asp Ala Asn Ile Gln 
                325                 330                 335     


Lys Lys Val Phe Asp Gln Trp Tyr Ala Asn Gly Thr Gly Pro Leu Ala 
            340                 345                 350         


Thr Asn Gly Ile Glu Ala Gly Val Lys Ile Arg Pro Thr Pro Glu Glu 
        355                 360                 365             


Leu Ser Gln Met Asp Glu Ser Phe Gln Glu Gly Tyr Arg Glu Tyr Phe 
    370                 375                 380                 


Glu Asp Lys Pro Asp Lys Pro Val Met His Tyr Ser Ile Ile Ala Gly 
385                 390                 395                 400 


Phe Phe Gly Asp His Thr Lys Ile Pro Pro Gly Lys Tyr Met Thr Met 
                405                 410                 415     


Phe His Phe Leu Glu Tyr Pro Phe Ser Arg Gly Ser Ile His Ile Thr 
            420                 425                 430         


Ser Pro Asp Pro Tyr Ala Thr Pro Asp Phe Asp Pro Gly Phe Met Asn 
        435                 440                 445             


Asp Glu Arg Asp Met Ala Pro Met Val Trp Ser Tyr Lys Lys Ser Arg 
    450                 455                 460                 


Glu Thr Ala Arg Lys Met Asp His Phe Ala Gly Glu Val Thr Ser His 
465                 470                 475                 480 


His Pro Leu Phe Pro Tyr Ser Ser Glu Ala Arg Ala Tyr Glu Met Asp 
                485                 490                 495     


Leu Glu Thr Ser Asn Ala Tyr Gly Gly Pro Leu Asn Leu Thr Ala Gly 
            500                 505                 510         


Leu Ala His Gly Ser Trp Thr Gln Pro Leu Lys Lys Pro Ala Gly Arg 
        515                 520                 525             


Asn Glu Gly His Val Thr Ser Asn Gln Val Glu Leu His Pro Asp Ile 
    530                 535                 540                 


Glu Tyr Asp Glu Glu Asp Asp Lys Ala Ile Glu Asn Tyr Ile Arg Glu 
545                 550                 555                 560 


His Thr Glu Thr Thr Trp His Cys Leu Gly Thr Cys Ser Ile Gly Pro 
                565                 570                 575     


Arg Glu Gly Ser Lys Ile Val Lys Trp Gly Gly Val Leu Asp His Arg 
            580                 585                 590         


Ser Asn Val Tyr Gly Val Lys Gly Leu Lys Val Gly Asp Leu Ser Val 
        595                 600                 605             


Cys Pro Asp Asn Val Gly Cys Asn Thr Tyr Thr Thr Ala Leu Leu Ile 
    610                 615                 620                 


Gly Glu Lys Thr Ala Thr Leu Val Gly Glu Asp Leu Gly Tyr Thr Gly 
625                 630                 635                 640 


Glu Ala Leu Asp Met Thr Val Pro Gln Phe Lys Leu Gly Thr Tyr Glu 
                645                 650                 655     


Lys Thr Gly Leu Ala Arg Phe 
            660             


<210>  2
<211>  1992
<212>  DNA
<213>  Komagataella phaffii

<400>  2
atggctatcc ccgaagagtt tgatatccta gttctaggtg gtggatccag tggatcctgt       60

attgccggaa gattggcaaa cttggaccac tccttgaaag ttggtcttat cgaagcaggt      120

gagaacaacc tcaacaaccc atgggtctac cttccaggta tttacccaag aaacatgaag      180

ttggactcca agactgcttc cttctacact tctaacccat ctcctcactt gaatggtaga      240

agagccattg ttccatgtgc taacgtcttg ggtggtggtt cttctatcaa cttcatgatg      300

tacaccagag gttctgcttc tgattacgat gacttccaag ccgagggctg gaaaaccaag      360

gacttgcttc cattgatgaa aaagactgag acctaccaaa gagcttgcaa caaccctgac      420

attcacggtt tcgaaggtcc aatcaaggtt tctttcggta actacaccta cccagtttgc      480

caggacttct tgagggcttc tgagtcccaa ggtattccat acgttgacga cttggaagac      540

ttggttactg ctcacggtgc tgaacactgg ttgaagtgga tcaacagaga cactggtcgt      600

cgttccgact ctgctcatgc atttgtccac tctactatga gaaaccacga caacttgtac      660

ttgatctgta acacgaaggt cgacaaaatt attgtcgaag acggaagagc tgctgctgtt      720

agaaccgttc caagcaagcc tttgaaccca aagaagccaa gtcacaagat ctaccgtgct      780

agaaagcaaa tcgttttgtc ttgtggtacc atctcctctc cattggtttt gcaaagatcc      840

ggttttggtg acccaatcaa gttgagagcc gctggtgtta agcctttggt caacttgcca      900

ggtgtcggaa gaaacttcca agaccactac tgtttcttca gtccttacag aatcaagcct      960

cagtacgagt ctttcgatga cttcgtccgt ggtgatgctg agattcaaaa gagagtcttt     1020

gaccaatggt acgccaatgg tactggtcct cttgccacta acggtatcga agctggtgtc     1080

aagatcagac caacaccaga agaactctct caaatggacg aatccttcca ggagggttac     1140

agagaatact tcgaagacaa gccagacaag ccagttatgc actactccat cattgctggt     1200

ttcttcggtg accacaccaa gattcctcct ggaaagtaca tgactatgtt ccacttcttg     1260

gaatacccat tctccagagg ttccattcac attacctccc cagacccata cgcagctcca     1320

gacttcgacc caggtttcat gaacgatgaa agagacatgg ctcctatggt ttgggcttac     1380

aagaagtcta gagaaaccgc tagaagaatg gaccactttg ccggtgaggt cacttctcac     1440

caccctctgt tcccatactc atccgaggcc agagccttgg aaatggattt ggagacctct     1500

aatgcctacg gtggaccttt gaacttgtct gctggtcttg ctcacggttc ttggactcaa     1560

cctttgaaga agccaactgc aaagaacgaa ggccacgtta cttcgaacca ggtcgagctt     1620

catccagaca tcgagtacga tgaggaggat gacaaggcca ttgagaacta cattcgtgag     1680

cacactgaga ccacatggca ctgtctggga acctgttcca tcggtccaag agaaggttcc     1740

aagatcgtca aatggggtgg tgttttggac cacagatcca acgtttacgg agtcaagggc     1800

ttgaaggttg gtgacttgtc cgtgtgccca gacaatgttg gttgtaacac ctacaccacc     1860

gctcttttga tcggtgaaaa gactgccact ttggttggag aagatttagg atactctggt     1920

gaggccttag acatgactgt tcctcagttc aagttgggca cttacgagaa gaccggtctt     1980

gctagattct aa                                                         1992


<210>  3
<211>  663
<212>  PRT
<213>  Komagataella phaffii

<400>  3

Met Ala Ile Pro Glu Glu Phe Asp Ile Leu Val Leu Gly Gly Gly Ser 
1               5                   10                  15      


Ser Gly Ser Cys Ile Ala Gly Arg Leu Ala Asn Leu Asp His Ser Leu 
            20                  25                  30          


Lys Val Gly Leu Ile Glu Ala Gly Glu Asn Asn Leu Asn Asn Pro Trp 
        35                  40                  45              


Val Tyr Leu Pro Gly Ile Tyr Pro Arg Asn Met Lys Leu Asp Ser Lys 
    50                  55                  60                  


Thr Ala Ser Phe Tyr Thr Ser Asn Pro Ser Pro His Leu Asn Gly Arg 
65                  70                  75                  80  


Arg Ala Ile Val Pro Cys Ala Asn Ile Leu Gly Gly Gly Ser Ser Ile 
                85                  90                  95      


Asn Phe Met Met Tyr Thr Arg Gly Ser Ala Ser Asp Tyr Asp Asp Phe 
            100                 105                 110         


Glu Ala Glu Gly Trp Lys Thr Lys Asp Leu Leu Pro Leu Met Lys Lys 
        115                 120                 125             


Thr Glu Thr Tyr Gln Arg Ala Cys Asn Asn Pro Glu Ile His Gly Phe 
    130                 135                 140                 


Glu Gly Pro Ile Lys Val Ser Phe Gly Asn Tyr Thr Tyr Pro Val Cys 
145                 150                 155                 160 


Gln Asp Phe Leu Arg Ala Thr Glu Ser Gln Gly Ile Pro Tyr Val Asp 
                165                 170                 175     


Asp Leu Glu Asp Leu Val Thr Ala His Gly Ala Glu His Trp Leu Lys 
            180                 185                 190         


Trp Ile Asn Arg Asp Thr Gly Arg Arg Ser Asp Ser Ala His Ala Phe 
        195                 200                 205             


Val His Ser Thr Met Arg Asn His Asp Asn Leu Tyr Leu Ile Cys Asn 
    210                 215                 220                 


Thr Lys Val Asp Lys Ile Ile Val Glu Asp Gly Arg Ala Ala Ala Val 
225                 230                 235                 240 


Arg Thr Val Pro Ser Lys Pro Leu Asn Ala Lys Lys Pro Thr His Lys 
                245                 250                 255     


Val Tyr Arg Ala Arg Lys Gln Ile Val Leu Ser Cys Gly Thr Ile Ser 
            260                 265                 270         


Ser Pro Leu Val Leu Gln Arg Ser Gly Phe Gly Asp Pro Ile Lys Leu 
        275                 280                 285             


Arg Ala Ala Gly Val Lys Pro Leu Val Asn Leu Pro Gly Val Gly Arg 
    290                 295                 300                 


Asn Phe Gln Asp His Tyr Cys Phe Phe Ser Pro Tyr Arg Ile Lys Pro 
305                 310                 315                 320 


Gln Tyr Glu Ser Phe Asp Asp Phe Val Arg Gly Asp Ala Asn Ile Gln 
                325                 330                 335     


Lys Lys Val Phe Asp Gln Trp Tyr Ala Asn Gly Thr Gly Pro Leu Ala 
            340                 345                 350         


Thr Asn Gly Ile Glu Ala Gly Val Lys Ile Arg Pro Thr Pro Glu Glu 
        355                 360                 365             


Leu Ser Gln Met Asp Glu Ser Phe Gln Glu Gly Tyr Arg Glu Tyr Phe 
    370                 375                 380                 


Glu Asp Lys Pro Asp Lys Pro Val Met His Tyr Ser Ile Ile Ala Gly 
385                 390                 395                 400 


Phe Phe Gly Asp His Thr Lys Ile Pro Pro Gly Lys Tyr Met Thr Met 
                405                 410                 415     


Phe His Phe Leu Glu Tyr Pro Phe Ser Arg Gly Ser Ile His Ile Thr 
            420                 425                 430         


Ser Pro Asp Pro Tyr Ala Thr Pro Asp Phe Asp Pro Gly Phe Met Asn 
        435                 440                 445             


Asp Glu Arg Asp Met Ala Pro Met Val Trp Ser Tyr Lys Lys Ser Arg 
    450                 455                 460                 


Glu Thr Ala Arg Lys Met Asp His Phe Ala Gly Glu Val Thr Ser His 
465                 470                 475                 480 


His Pro Leu Phe Pro Tyr Ser Ser Glu Ala Arg Ala Tyr Glu Met Asp 
                485                 490                 495     


Leu Glu Thr Ser Asn Ala Tyr Gly Gly Pro Leu Asn Leu Thr Ala Gly 
            500                 505                 510         


Leu Ala His Gly Ser Trp Thr Gln Pro Leu Lys Lys Pro Ala Gly Arg 
        515                 520                 525             


Asn Glu Gly His Val Thr Ser Asn Gln Val Glu Leu His Pro Asp Ile 
    530                 535                 540                 


Glu Tyr Asp Glu Glu Asp Asp Lys Ala Ile Glu Asn Tyr Ile Arg Glu 
545                 550                 555                 560 


His Thr Glu Thr Thr Trp His Cys Leu Gly Thr Cys Ser Ile Gly Pro 
                565                 570                 575     


Arg Glu Gly Ser Lys Ile Val Lys Trp Gly Gly Val Leu Asp His Arg 
            580                 585                 590         


Ser Asn Val Tyr Gly Val Lys Gly Leu Lys Val Gly Asp Leu Ser Val 
        595                 600                 605             


Cys Pro Asp Asn Val Gly Cys Asn Thr Tyr Thr Thr Ala Leu Leu Ile 
    610                 615                 620                 


Gly Glu Lys Thr Ala Thr Leu Val Gly Glu Asp Leu Gly Tyr Thr Gly 
625                 630                 635                 640 


Glu Ala Leu Asp Met Thr Val Pro Gln Phe Lys Leu Gly Thr Tyr Glu 
                645                 650                 655     


Lys Thr Gly Leu Ala Arg Phe 
            660             


<210>  4
<211>  1992
<212>  DNA
<213>  Komagataella phaffii

<400>  4
atggccattc ctgaagaatt cgatattctt gtcctgggtg gtggatccag tggatcctgt       60

attgccggaa gattggccaa cttggaccac tccttgaaag ttggtcttat cgaggctggt      120

gagaacaatc ttaacaaccc atgggtctac cttccaggta tttacccaag aaacatgaag      180

ttggactcca aaactgcttc tttctacacc tccaaccctt ctcctcattt gaatggtaga      240

agagctattg tcccatgtgc caacatcttg ggtggtggtt cttcgatcaa cttcatgatg      300

tacaccagag gttccgcttc tgattacgat gactttgaag ctgagggatg gaagaccaag      360

gatttgcttc ctttgatgaa gaagactgag acttaccaaa gagcttgcaa caaccctgaa      420

attcacggtt ttgaaggtcc aatcaaggtt tctttcggta actacactta cccagtttgt      480

caagacttct tgagagcaac tgaatcccaa ggtattccat acgttgacga cttggaagac      540

ttggtgactg ctcatggtgc tgaacactgg ctgaaatgga tcaacagaga cactggtcgt      600

cgttccgact ctgctcatgc cttcgttcat tctacgatga gaaaccacga caatctgtac      660

ttgatctgca acaccaaagt tgacaagatt attgttgaag acggaagagc tgctgctgtc      720

agaaccgttc caagtaaacc tttgaacgca aagaagccaa ctcacaaggt ttatcgtgct      780

agaaagcaaa tcgttttgtc ttgtggtacc atctcttctc ctctggttct gcaaagatcc      840

ggttttggtg acccaatcaa attgagagcc gctggtgtta agcctttggt caacttgcca      900

ggtgttggaa gaaacttcca agaccactac tgcttcttct ctccttacag aattaagccc      960

caatacgagt ctttcgatga cttcgtacgt ggtgacgcta acattcaaaa gaaggtattc     1020

gaccaatggt acgctaacgg tactggtcca ttggccacca acggtattga agccggtgtc     1080

aagattagac caactccaga agaattatct cagatggacg agtccttcca agagggttac     1140

agagagtact tcgaagacaa accagacaag ccagttatgc actattccat cattgctggt     1200

ttcttcggtg accacaccaa gattccacct ggaaagtaca tgaccatgtt ccacttcttg     1260

gagtacccat tctccagagg ttctatccac atcacctctc cagacccata cgcaactcca     1320

gactttgacc caggtttcat gaacgatgaa agagacatgg ctcctatggt ctggtcttac     1380

aagaagtcca gagagactgc cagaaaaatg gaccactttg ctggtgaggt tacttcccac     1440

caccctctgt tcccatactc atccgaggcc agagcttacg agatggattt ggagacctcc     1500

aacgcctacg gtggaccact gaacttgact gctggtcttg ctcacggttc ttggactcag     1560

cctttgaaga agcctgctgg aagaaacgaa ggacatgtta cttccaacca agtcgagctt     1620

catccagaca ttgagtacga tgaggaggat gataaggcca ttgagaacta cattcgtgag     1680

cacactgaga ccacatggca ctgtctggga acctgttcca ttggtccaag agagggttcc     1740

aagatcgtca aatggggtgg tgttttggat cacagatcta acgtttacgg agtcaagggc     1800

ctgaaggttg gtgacttgtc cgtctgtcca gacaatgttg gttgtaacac ctacaccacc     1860

gctcttttga tcggtgaaaa gactgccacc ttggttggtg aagacttagg atacacaggt     1920

gaggccttag acatgactgt acctcagttc aagttgggca cttacgagaa gactggtctt     1980

gctagattct ag                                                         1992


<210>  5
<211>  1000
<212>  DNA
<213>  Komagataella phaffii

<400>  5
catgttggta ttgtgaaata gacgcagatc gggaacactg aaaaataaca gttattattc       60

gagatctaac atccaaagac gaaaggttga atgaaacctt tttgccatcc gacatccaca      120

ggtccattct cacacataag tgccaaacgc aacaggaggg gatacactag cagcagaccg      180

ttgcaaacgc aggacctcca ctcctcttct cctcaacacc cacttttgcc atcgaaaaac      240

cagcccagtt attgggcttg attggagctc gctcattcca attccttcta ttaggctact      300

aacaccatga ctttattagc ctgtctatcc tggcccccct ggcgaggttc atgtttgttt      360

atttccgaat gcaacaagct ccgcattaca cccgaacatc actccagatg agggctttct      420

gagtgtgggg tcaaatagtt tcatgttccc caaatggccc aaaactgaca gtttaaacgc      480

tgtcttggaa cctaatatga caaaagcgtg atctcatcca agatgaacta agtttggttc      540

gttgaaatgc taacggccag ttggtcaaaa agaaacttcc aaaagtcggc ataccgtttg      600

tcttgtttgg tattgattga cgaatgctca aaaataatct cattaatgct tagcgcagtc      660

tctctatcgc ttctgaaccc cggtgcacct gtgccgaaac gcaaatgggg aaacacccgc      720

tttttggatg attatgcatt gtctccacat tgtatgcttc caagattctg gtgggaatac      780

tgctgatagc ctaacgttca tgatcaaaat ttaactgttc taacccctac ttgacagcaa      840

tatataaaca gaaggaagct gccctgtctt aaaccttttt tttatcatca ttattagctt      900

actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga      960

caacttgaga agatcaaaaa acaactaatt attcgaaacg                           1000


<210>  6
<211>  1000
<212>  DNA
<213>  Komagataella phaffii

<400>  6
gcttaaagga ctccatttcc taaaatttca agcagtcctc tcaactaaat ttttttccat       60

tcctctgcac ccagccctct tcatcaaccg tccagccttc tcaaaagtcc aatgtaagta      120

gcctgcaaat tcaggttaca acccctcaat tttccatcca agggcgatcc ttacaaagtt      180

aatatcgaac agcagagact aagcgagtca tcatcaccac ccaacgatgg tgaaaaactt      240

taagcataga ttgatggagg gtgtatggca cttggcggct gcattagagt ttgaaactat      300

ggggtaatac atcacatccg gaactgatcc gactccgaga tcatatgcaa agcacgtgat      360

gtaccccgta aactgctcgg attatcgttg caattcatcg tcttaaacag tacaagaaac      420

tttattcatg ggtcattgga ctctgatgag gggcacattt ccccaatgat tttttgggaa      480

agaaagccgt aagaggacag ttaagcgaaa gagacaagac aacgaacagc aaaagtgaca      540

gctgtcagct acctagtgga cagttgggag tttccaattg gttggttttg aatttttacc      600

catgttgagt tgtccttgct tctccttgca aacaatgcaa gttgataaga catcaccttc      660

caagataggc tatttttgtc gcataaattt ttgtctcgga gtgaaaaccc cttttatgtg      720

aacagattac agaagcgtcc tacccttcac cggttgagat ggggagaaaa ttaagcgatg      780

aggagacgat tattggtata aaagaagcaa ccaaaatccc ttattgtcct tttctgatca      840

gcatcaaaga atattgtctt aaaacgggct tttaactaca ttgttcttac acattgcaaa      900

cctcttcctt ctatttcgga tcaactgtat tgactacatt gatctttttt aacgaagttt      960

acgacttact aaatccccac aaacaaatca actgagaaaa                           1000


<210>  7
<211>  1000
<212>  DNA
<213>  Komagataella pastoris

<400>  7
atatcgtgaa atagacccaa atccggacac tgtgaaataa aacagttagt atgcgaaatc       60

taacatccaa gaacgagaaa ctaaataaga cattttgcca tccgacatct acaaaccaca      120

tcaccctcac acataagtgc caaaacgcag caggagggac acccagcagc agaagccgtg      180

tcgaacgcag gacctccact tctcttctcc tcaacatcca ctttcgttat tgaaaaccag      240

cctgcttaaa aaaactgatt ggagctcgct cattccagtc ccctttgtta ggctactaag      300

accacgactt tattagcctg tccattctgg ttcctggcga gacttattct tgtttgttta      360

ttttcgaatg caacaaagct ccgcattaca tccgaacatc actttagatg agggctttct      420

gagtgtgggg tcgaatagtt tcatgttccc ccaatggccc aaaactgaca ctttaaacgc      480

tgtcttcgaa cttaatatgg caaaagcgtg atctcatcca agacgaacta agtttggttc      540

gttgaaatgc taacggccag ttggtcaaaa agaaacttcc aaaagtcggc atatcgtttg      600

tcttgtttgg tattcataga cgaatgctca agaatattct cattaatgct tagcgcagtc      660

tctgtatcgc ttctggaccc cggtgcagtt gtgccgaaac gcaaatgggg aaacacccgc      720

ttttcggatg attatgcatt gtctccacat tgtatgcttc caagattctg gtgggaatac      780

tactgatagc ctaacgttca tgatcaatat caaactgttc taacccctac ttgaactgca      840

atatataaac aggaggaaac ttcccagtcg aaaaccttct ttcatcatca ttattagctt      900

actttcataa ttgtgactgg ttccaattga caagcttttg attctaacga cttttaacga      960

caatttgaga agatcaaaaa acaactaatt attcgaaacg                           1000


<210>  8
<211>  1000
<212>  DNA
<213>  Komagataella pastoris

<400>  8
gcttgcacga ctcagttacc tgaaaatttc agcctgtcct ctttaataaa atttcacccg       60

ttcctctgca tgtccactca gctctattca tctatccttg agccttctcg agcgtctaat      120

gaacagcctg cgaattcagg ttacaacccc tcattttttc gtctcggtcg atctctacaa      180

agtcaacagc caactttgat gttaagcgag tcatcaccag ccagcgatag tgaaaaactt      240

taagcataga ttgatggtgg gtttatgtca cttggcggct gcattagagt ttgaaactat      300

ggggtaatgc atcacatccg gaactgatcc gactcggaga tcatatgcaa accacgtgat      360

gtaccccgta aactgctcgg attactgttc caattcatcg tcttaaacag tataagaaac      420

tttattcatg ggtcattgga ctctgatgag gggcacattt ccccattgat ttttgggaca      480

gtaagccata aaaggactgt taagcgaagc agacaagaca acgaacagct agaataacaa      540

ctatctaccg ccttgtggac cgttgggagt ttccaattgg ttggttttgg atttctgagc      600

ccatgttgtg ttgtccatgc ttctccttgc acacaatgca agttgataag atatcacctt      660

ccaagatagg ctatttttgt cgcataaatt ttggtctcag agtgaaaccc ccttttatgt      720

gaacggatta gagaagcctc ctacccttca ccggctgaga tggggagaaa ttaagcgatg      780

aggagacgat aattgctata aaagaagcaa ccaaaacccc ttattgtctc tttctgatca      840

gcatcaaaga atattgtctt aaaacgggct tttaactaca ttgttcttac acattgcaaa      900

cctcctcctt caatttcgga tcagctgtat tgactacatt gatctttttt aacgaagttc      960

acgacttact aaatccccat aaacaaacca actgagaaaa                           1000


<210>  9
<211>  663
<212>  PRT
<213>  Komagataella pastoris

<400>  9

Met Ala Ile Pro Glu Glu Phe Asp Ile Leu Val Leu Gly Gly Gly Ser 
1               5                   10                  15      


Ser Gly Ser Cys Ile Ala Gly Arg Leu Ala Asn Leu Asp His Ser Leu 
            20                  25                  30          


Lys Val Gly Leu Ile Glu Ala Gly Glu Asn Asn Leu Asn Asn Pro Trp 
        35                  40                  45              


Val Tyr Leu Pro Gly Ile Tyr Pro Arg Asn Met Lys Leu Asp Ser Lys 
    50                  55                  60                  


Thr Ala Ser Phe Tyr Thr Ser Asn Pro Ser Pro His Leu Asn Gly Arg 
65                  70                  75                  80  


Arg Ala Ile Val Pro Cys Ala Asn Val Leu Gly Gly Gly Ser Ser Ile 
                85                  90                  95      


Asn Phe Met Met Tyr Thr Arg Gly Ser Ala Ser Asp Tyr Asp Asp Phe 
            100                 105                 110         


Gln Ala Glu Gly Trp Lys Thr Lys Asp Leu Leu Pro Leu Met Lys Lys 
        115                 120                 125             


Thr Glu Thr Tyr Gln Arg Ala Cys Asn Asn Pro Asp Ile His Gly Phe 
    130                 135                 140                 


Glu Gly Pro Ile Lys Val Ser Phe Gly Asn Tyr Thr Tyr Pro Val Cys 
145                 150                 155                 160 


Gln Asp Phe Leu Arg Ala Ser Glu Ser Gln Gly Ile Pro Tyr Val Asp 
                165                 170                 175     


Asp Leu Glu Asp Leu Val Thr Ala His Gly Ala Glu His Trp Leu Lys 
            180                 185                 190         


Trp Ile Asn Arg Asp Thr Gly Arg Arg Ser Asp Ser Ala His Ala Phe 
        195                 200                 205             


Val His Ser Thr Met Arg Asn His Asp Asn Leu Tyr Leu Ile Cys Asn 
    210                 215                 220                 


Thr Lys Val Asp Lys Ile Ile Val Glu Asp Gly Arg Ala Ala Ala Val 
225                 230                 235                 240 


Arg Thr Val Pro Ser Lys Pro Leu Asn Pro Lys Lys Pro Ser His Lys 
                245                 250                 255     


Ile Tyr Arg Ala Arg Lys Gln Ile Val Leu Ser Cys Gly Thr Ile Ser 
            260                 265                 270         


Ser Pro Leu Val Leu Gln Arg Ser Gly Phe Gly Asp Pro Ile Lys Leu 
        275                 280                 285             


Arg Ala Ala Gly Val Lys Pro Leu Val Asn Leu Pro Gly Val Gly Arg 
    290                 295                 300                 


Asn Phe Gln Asp His Tyr Cys Phe Phe Ser Pro Tyr Arg Ile Lys Pro 
305                 310                 315                 320 


Gln Tyr Glu Ser Phe Asp Asp Phe Val Arg Gly Asp Ala Glu Ile Gln 
                325                 330                 335     


Lys Arg Val Phe Asp Gln Trp Tyr Ala Asn Gly Thr Gly Pro Leu Ala 
            340                 345                 350         


Thr Asn Gly Ile Glu Ala Gly Val Lys Ile Arg Pro Thr Pro Glu Glu 
        355                 360                 365             


Leu Ser Gln Met Asp Glu Ser Phe Gln Glu Gly Tyr Arg Glu Tyr Phe 
    370                 375                 380                 


Glu Asp Lys Pro Asp Lys Pro Val Met His Tyr Ser Ile Ile Ala Gly 
385                 390                 395                 400 


Phe Phe Gly Asp His Thr Lys Ile Pro Pro Gly Lys Tyr Met Thr Met 
                405                 410                 415     


Phe His Phe Leu Glu Tyr Pro Phe Ser Arg Gly Ser Ile His Ile Thr 
            420                 425                 430         


Ser Pro Asp Pro Tyr Ala Ala Pro Asp Phe Asp Pro Gly Phe Met Asn 
        435                 440                 445             


Asp Glu Arg Asp Met Ala Pro Met Val Trp Ala Tyr Lys Lys Ser Arg 
    450                 455                 460                 


Glu Thr Ala Arg Arg Met Asp His Phe Ala Gly Glu Val Thr Ser His 
465                 470                 475                 480 


His Pro Leu Phe Pro Tyr Ser Ser Glu Ala Arg Ala Leu Glu Met Asp 
                485                 490                 495     


Leu Glu Thr Ser Asn Ala Tyr Gly Gly Pro Leu Asn Leu Ser Ala Gly 
            500                 505                 510         


Leu Ala His Gly Ser Trp Thr Gln Pro Leu Lys Lys Pro Thr Ala Lys 
        515                 520                 525             


Asn Glu Gly His Val Thr Ser Asn Gln Val Glu Leu His Pro Asp Ile 
    530                 535                 540                 


Glu Tyr Asp Glu Glu Asp Asp Lys Ala Ile Glu Asn Tyr Ile Arg Glu 
545                 550                 555                 560 


His Thr Glu Thr Thr Trp His Cys Leu Gly Thr Cys Ser Ile Gly Pro 
                565                 570                 575     


Arg Glu Gly Ser Lys Ile Val Lys Trp Gly Gly Val Leu Asp His Arg 
            580                 585                 590         


Ser Asn Val Tyr Gly Val Lys Gly Leu Lys Val Gly Asp Leu Ser Val 
        595                 600                 605             


Cys Pro Asp Asn Val Gly Cys Asn Thr Tyr Thr Thr Ala Leu Leu Ile 
    610                 615                 620                 


Gly Glu Lys Thr Ala Thr Leu Val Gly Glu Asp Leu Gly Tyr Thr Gly 
625                 630                 635                 640 


Glu Ala Leu Asp Met Thr Val Pro Gln Phe Lys Leu Gly Thr Tyr Glu 
                645                 650                 655     


Lys Thr Gly Leu Ala Arg Phe 
            660             


<210>  10
<211>  1992
<212>  DNA
<213>  Komagataella pastoris

<400>  10
atggctatcc ctgaagagtt tgatatcctt gttttaggtg gtggatccag tggatcctgt       60

attgccggaa gattggccaa cttggaccac tccttgaaag ttggtcttat cgaggcaggt      120

gagaacaacc tcaacaaccc atgggtttac cttccaggta tttacccaag aaacatgaag      180

ttggactcca agactgcatc cttctacact tctaaccctt ctcctcactt gaacggtaga      240

agagctattg ttccatgtgc taacgtcttg ggtggtggtt cttccattaa cttcatgatg      300

tacaccagag gttctgcttc tgattatgac gacttccaag ccgagggctg gaaaaccaag      360

gacttgcttc cattgatgaa aaagaccgag acctaccaaa gagcttgcaa caaccctgac      420

attcacgggt tcgaaggtcc aatcaaggtt tctttcggta actacaccta cccagtttgc      480

caggacttct tgagagcttc tgaatcccaa ggtattccat acgttgacga cttggaagac      540

ttggttactg ctcacggtgc tgaacactgg ctgaaatgga tcaacagaga cactggtcgt      600

cgttccgact ccgctcatgc atttgtccac tctactatga gaaaccacga caacttgtac      660

ttgatttgta acacaaaggt tgacaagatt attgtcgaag acggaagagc tgctgctgtt      720

agaactgttc caagcaagcc tttgaaccca aagaagccaa gtcacaagat ctaccgtgct      780

agaaagcaaa tcgttttgtc ttgtggtacc atctcatctc ctttggttct gcaaagatcc      840

ggtttcggtg acccaatcaa gttgagagcc gctggtgtta agcctttggt caacttgcct      900

ggtgtcggaa gaaacttcca agaccactac tgtttcttca gtccttacag aatcaagcct      960

cagtacgaat ctttcgatga cttcgtgcgt ggtgatgctg agatccaaaa gagagttttc     1020

gaccaatggt acgccaatgg tactggtcct cttgccacta acggtatcga agccggtgtc     1080

aagattagac caacaccaga ggaactgtct caaatggacg aatctttcca agagggttac     1140

agagaatact ttgaggacaa gccagacaag ccagttatgc actactccat tattgctggt     1200

ttcttcggtg accacaccaa gattcctcct ggaaagtaca tgaccatgtt ccactttttg     1260

gaatacccat tctccagagg ttccattcac attacctctc cagatccata cgcagctcca     1320

gacttcgacc caggtttcat gaacgatgaa agagacatgg ctcctatggt ctgggcctac     1380

aagaagtcta gagagacagc tagaagaatg gaccactttg ccggtgaggt tacttctcac     1440

cacccattgt tcccatactc atccgaggcc agagctttgg agatggattt ggagacctcc     1500

aatgcctacg gtggaccttt gaacttgtct gctggtcttg cccacggttc ttggactcaa     1560

cctttgaaga agccaactgc aaagaacgaa ggccatgtta cctccaacca agtcgagctt     1620

catccagaca tcgagtacga cgaggaggac gacaaggcca ttgaaaacta catccgtgag     1680

cacactgaga ccacatggca ctgtctggga acctgttcca tcggtccaag agagggttcc     1740

aagatcgtca aatggggtgg tgttttggac cacagatcca acgtttacgg agtcaagggc     1800

ctgaaggttg gtgacttgtc tgtctgtcca gacaatgttg gttgtaacac ctacaccacc     1860

gctcttttga tcggtgagaa gactgccact ttggttggag aagacttagg atacaccggt     1920

gaagccttag acatgactgt tcctcagttc aagttgggca cttacgagaa gaccggtctt     1980

gctagattct aa                                                         1992


<210>  11
<211>  663
<212>  PRT
<213>  Komagataella pastoris

<400>  11

Met Ala Ile Pro Glu Glu Phe Asp Ile Leu Val Leu Gly Gly Gly Ser 
1               5                   10                  15      


Ser Gly Ser Cys Ile Ala Gly Arg Leu Ala Asn Leu Asp His Ser Leu 
            20                  25                  30          


Lys Val Gly Leu Ile Glu Ala Gly Glu Asn Asn Leu Asn Asn Pro Trp 
        35                  40                  45              


Val Tyr Leu Pro Gly Ile Tyr Pro Arg Asn Met Lys Leu Asp Ser Lys 
    50                  55                  60                  


Thr Ala Ser Phe Tyr Thr Ser Asn Pro Ser Pro His Leu Asn Gly Arg 
65                  70                  75                  80  


Arg Ala Ile Val Pro Cys Ala Asn Ile Leu Gly Gly Gly Ser Ser Ile 
                85                  90                  95      


Asn Phe Met Met Tyr Thr Arg Gly Ser Ala Ser Asp Tyr Asp Asp Phe 
            100                 105                 110         


Glu Ala Glu Gly Trp Lys Thr Lys Asp Leu Leu Pro Leu Met Lys Lys 
        115                 120                 125             


Thr Glu Thr Tyr Gln Arg Ala Cys Asn Asn Pro Glu Ile His Gly Phe 
    130                 135                 140                 


Glu Gly Pro Ile Lys Val Ser Phe Gly Asn Tyr Thr Tyr Pro Val Cys 
145                 150                 155                 160 


Gln Asp Phe Leu Arg Ala Thr Glu Ser Gln Gly Ile Pro Tyr Val Asp 
                165                 170                 175     


Asp Leu Glu Asp Leu Glu Thr Ala His Gly Ala Glu His Trp Leu Lys 
            180                 185                 190         


Trp Ile Asn Arg Asp Thr Gly Arg Arg Ser Asp Ser Ala His Ala Phe 
        195                 200                 205             


Val His Ser Thr Met Arg Asn His Asp Asn Leu Tyr Leu Ile Cys Asn 
    210                 215                 220                 


Thr Lys Val Asp Lys Ile Ile Val Glu Asp Gly Arg Ala Ala Gly Val 
225                 230                 235                 240 


Arg Thr Val Pro Ser Lys Pro Leu Asn Ala Lys Lys Pro Thr His Lys 
                245                 250                 255     


Val Tyr Arg Ala Arg Lys Gln Ile Val Leu Ser Cys Gly Thr Ile Ser 
            260                 265                 270         


Ser Pro Leu Val Leu Gln Arg Ser Gly Phe Gly Asp Pro Ile Lys Leu 
        275                 280                 285             


Arg Ala Ala Gly Val Lys Pro Leu Val Asn Leu Pro Gly Val Gly Arg 
    290                 295                 300                 


Asn Phe Gln Asp His Tyr Cys Phe Phe Ser Pro Tyr Arg Ile Lys Pro 
305                 310                 315                 320 


Gln Tyr Glu Ser Phe Asp Asp Phe Val Arg Gly Asp Ala Asn Ile Gln 
                325                 330                 335     


Lys Lys Val Phe Asp Gln Trp Tyr Ala Asn Gly Thr Gly Pro Leu Ala 
            340                 345                 350         


Thr Asn Gly Ile Glu Ala Gly Val Lys Ile Arg Pro Thr Pro Glu Glu 
        355                 360                 365             


Leu Ser Gln Met Asp Glu Ser Phe Gln Glu Gly Tyr Arg Glu Tyr Phe 
    370                 375                 380                 


Glu Asp Lys Pro Asp Lys Pro Val Met His Tyr Ser Ile Ile Ala Gly 
385                 390                 395                 400 


Phe Phe Gly Asp His Thr Lys Ile Pro Pro Gly Lys Tyr Met Thr Met 
                405                 410                 415     


Phe His Phe Leu Glu Tyr Pro Phe Ser Arg Gly Ser Ile His Ile Thr 
            420                 425                 430         


Ser Pro Asp Pro Tyr Ala Thr Pro Asp Phe Asp Pro Gly Phe Met Asn 
        435                 440                 445             


Asp Glu Arg Asp Met Ala Pro Met Val Trp Ser Tyr Lys Lys Ser Arg 
    450                 455                 460                 


Glu Thr Ala Arg Lys Met Asp His Phe Ala Gly Glu Val Thr Ser His 
465                 470                 475                 480 


His Pro Leu Phe Pro Tyr Ser Ser Glu Ala Arg Ala Tyr Glu Met Asp 
                485                 490                 495     


Leu Glu Thr Ser Asn Ala Tyr Gly Gly Pro Leu Asn Leu Thr Ala Gly 
            500                 505                 510         


Leu Ala His Gly Ser Trp Thr Gln Pro Leu Lys Lys Pro Ala Ala Arg 
        515                 520                 525             


Asn Glu Gly His Val Thr Ser Asn Gln Val Glu Leu His Pro Asp Ile 
    530                 535                 540                 


Glu Tyr Asp Glu Glu Asp Asp Lys Ala Ile Glu Asn Tyr Ile Arg Glu 
545                 550                 555                 560 


His Thr Glu Thr Thr Trp His Cys Leu Gly Thr Cys Ser Ile Gly Pro 
                565                 570                 575     


Arg Glu Gly Ser Lys Ile Val Lys Trp Gly Gly Val Leu Asp His Arg 
            580                 585                 590         


Ser Asn Val Tyr Gly Val Lys Gly Leu Lys Val Gly Asp Leu Ser Val 
        595                 600                 605             


Cys Pro Asp Asn Val Gly Cys Asn Thr Tyr Thr Thr Ala Leu Leu Ile 
    610                 615                 620                 


Gly Glu Lys Thr Ala Thr Leu Val Gly Glu Asp Leu Gly Tyr Thr Gly 
625                 630                 635                 640 


Asp Ala Leu Asp Met Thr Val Pro Gln Phe Lys Leu Gly Thr Tyr Glu 
                645                 650                 655     


Lys Thr Gly Leu Ala Arg Phe 
            660             


<210>  12
<211>  1992
<212>  DNA
<213>  Komagataella pastoris

<400>  12
atggctattc ctgaagaatt cgatattctt gtcctaggtg gtggatccag tggatcctgt       60

attgccggaa gattggccaa cttggaccac tctttgaaag ttggtcttat cgaggccggt      120

gagaacaatc ttaacaaccc ttgggtctac cttccaggta tttacccaag aaacatgaaa      180

ttggactcca agaccgcttc tttctacacc tccaacccat ctcctcattt gaatggtaga      240

agagctattg tcccatgtgc taacatcttg ggtggtggtt cttccatcaa cttcatgatg      300

tacaccagag gttccgcttc tgattacgat gacttcgaag ctgagggctg gaaaaccaag      360

gatttgcttc ctttgatgaa gaagactgag acctaccaaa gagcttgcaa caaccctgag      420

atccacggtt tcgaaggtcc aatcaaggtt tctttcggta actacactta cccggtttgt      480

caagacttct tgagagcaac tgaatcccaa ggtattccat acgttgacga cttggaagac      540

ttggagactg ctcatggtgc cgaacactgg ttgaaatgga tcaacagaga cactggtcgt      600

cgttccgact ctgctcatgc tttcgtccat tctactatga gaaaccatga taacttgtac      660

ttgatctgca acaccaaggt tgacaagatt attgttgaag acggaagagc tgctggtgtc      720

agaaccgtcc caagtaaacc tttgaacgca aagaagccaa ctcacaaggt ttaccgtgct      780

agaaagcaga tcgttttgtc ttgtggtacc atttcttccc ctctggtttt gcaaagatcc      840

ggttttggtg atccaatcaa attgagagcc gctggtgtta agcctttggt caacttgcca      900

ggtgttggaa ggaacttcca ggaccattac tgcttcttct ctccttacag aatcaagccc      960

caatacgagt cttttgatga cttcgtccgt ggtgacgcta acatccaaaa gaaggtattc     1020

gaccaatggt acgctaacgg tactggtcca ttggccacca atggtattga agccggtgtc     1080

aagatcagac caactccaga ggaattatct caaatggacg agtcgttcca ggagggttac     1140

agagagtact ttgaagacaa accagacaaa ccagttatgc actattccat cattgctggt     1200

ttcttcggtg accacaccaa gattccgcct ggaaagtaca tgaccatgtt ccacttcttg     1260

gagtacccat tctccagagg ttctattcat atcacctctc cagacccata cgcaactcca     1320

gactttgacc caggtttcat gaatgatgaa agagacatgg ctcctatggt ttggtcttac     1380

aagaagtcca gagagactgc cagaaagatg gatcactttg ctggtgaggt tacttcccac     1440

caccctctgt tcccatactc atccgaggcc agagcttacg agatggactt ggagacctcc     1500

aacgcctacg gtggaccact gaacttgact gctggtcttg ctcacggttc ttggactcag     1560

cctttgaaga agcctgccgc aagaaacgaa ggacatgtta cctctaacca agttgagctt     1620

catccagaca ttgaatacga tgaggaggat gacaaggcca ttgagaacta catccgtgag     1680

cacactgaga ccacatggca ctgtctcgga acctgttcca tcggtccaag agaaggttcc     1740

aagatagtca aatggggtgg tgttttggac cacagatcca acgtttacgg agtcaagggc     1800

ctgaaggttg gtgacttgtc tgtctgccca gacaatgttg gttgtaacac ctacaccacc     1860

gctcttttaa tcggtgaaaa gactgcaacc ttggtgggtg aagacttagg atacacaggt     1920

gatgccttag acatgactgt tcctcagttc aagttgggca cttacgagaa gactggtctt     1980

gctagattct ag                                                         1992


<210>  13
<211>  664
<212>  PRT
<213>  Ogataea methanolica

<400>  13

Met Ala Ile Pro Asp Glu Phe Asp Ile Ile Val Val Gly Gly Gly Ser 
1               5                   10                  15      


Thr Gly Cys Ala Leu Ala Gly Arg Leu Gly Asn Leu Asp Glu Asn Val 
            20                  25                  30          


Thr Val Ala Leu Ile Glu Gly Gly Glu Asn Asn Ile Asn Asn Pro Trp 
        35                  40                  45              


Val Tyr Leu Pro Gly Val Tyr Pro Arg Asn Met Arg Leu Asp Ser Lys 
    50                  55                  60                  


Thr Ala Thr Phe Tyr Ser Ser Arg Pro Ser Pro His Leu Asn Gly Arg 
65                  70                  75                  80  


Arg Ala Ile Val Pro Cys Ala Asn Ile Leu Gly Gly Gly Ser Ser Ile 
                85                  90                  95      


Asn Phe Leu Met Tyr Thr Arg Ala Ser Ala Ser Asp Tyr Asp Asp Trp 
            100                 105                 110         


Glu Ser Glu Gly Trp Thr Thr Asp Glu Leu Leu Pro Leu Met Lys Lys 
        115                 120                 125             


Ile Glu Thr Tyr Gln Arg Pro Cys Asn Asn Arg Glu Leu His Gly Phe 
    130                 135                 140                 


Asp Gly Pro Ile Lys Val Ser Phe Gly Asn Tyr Thr Tyr Pro Asn Gly 
145                 150                 155                 160 


Gln Asp Phe Ile Arg Ala Ala Glu Ser Gln Gly Ile Pro Phe Val Asp 
                165                 170                 175     


Asp Ala Glu Asp Leu Lys Cys Ser His Gly Ala Glu His Trp Leu Lys 
            180                 185                 190         


Trp Ile Asn Arg Asp Leu Gly Arg Arg Ser Asp Ser Ala His Ala Tyr 
        195                 200                 205             


Ile His Pro Thr Met Arg Asn Lys Gln Asn Leu Phe Leu Ile Thr Ser 
    210                 215                 220                 


Thr Lys Cys Glu Lys Ile Ile Ile Glu Asn Gly Val Ala Thr Gly Val 
225                 230                 235                 240 


Lys Thr Val Pro Met Lys Pro Thr Gly Ser Pro Lys Thr Gln Val Ala 
                245                 250                 255     


Arg Thr Phe Lys Ala Arg Lys Gln Ile Ile Val Ser Cys Gly Thr Ile 
            260                 265                 270         


Ser Ser Pro Leu Val Leu Gln Arg Ser Gly Ile Gly Ser Ala His Lys 
        275                 280                 285             


Leu Arg Gln Val Gly Ile Lys Pro Ile Val Asp Leu Pro Gly Val Gly 
    290                 295                 300                 


Met Asn Phe Gln Asp His Tyr Cys Phe Phe Thr Pro Tyr His Val Lys 
305                 310                 315                 320 


Pro Asp Thr Pro Ser Phe Asp Asp Phe Val Arg Gly Asp Lys Ala Val 
                325                 330                 335     


Gln Lys Ser Ala Phe Asp Gln Trp Tyr Ala Asn Lys Asp Gly Pro Leu 
            340                 345                 350         


Thr Thr Asn Gly Ile Glu Ala Gly Val Lys Ile Arg Pro Thr Glu Glu 
        355                 360                 365             


Glu Leu Ala Thr Ala Asp Asp Glu Phe Arg Ala Ala Tyr Asp Asp Tyr 
    370                 375                 380                 


Phe Gly Asn Lys Pro Asp Lys Pro Leu Met His Tyr Ser Leu Ile Ser 
385                 390                 395                 400 


Gly Phe Phe Gly Asp His Thr Lys Ile Pro Asn Gly Lys Tyr Met Cys 
                405                 410                 415     


Met Phe His Phe Leu Glu Tyr Pro Phe Ser Arg Gly Phe Val His Val 
            420                 425                 430         


Val Ser Pro Asn Pro Tyr Asp Ala Pro Asp Phe Asp Pro Gly Phe Met 
        435                 440                 445             


Asn Asp Pro Arg Asp Met Trp Pro Met Val Trp Ser Tyr Lys Lys Ser 
    450                 455                 460                 


Arg Glu Thr Ala Arg Arg Met Asp Cys Phe Ala Gly Glu Val Thr Ser 
465                 470                 475                 480 


His His Pro His Tyr Pro Tyr Asp Ser Pro Ala Arg Ala Ala Asp Met 
                485                 490                 495     


Asp Leu Glu Thr Thr Lys Ala Tyr Ala Gly Pro Asp His Phe Thr Ala 
            500                 505                 510         


Asn Leu Tyr His Gly Ser Trp Thr Val Pro Ile Glu Lys Pro Thr Pro 
        515                 520                 525             


Lys Asn Ala Ala His Val Thr Ser Asn Gln Val Glu Lys His Arg Asp 
    530                 535                 540                 


Ile Glu Tyr Thr Lys Glu Asp Asp Ala Ala Ile Glu Asp Tyr Ile Arg 
545                 550                 555                 560 


Glu His Thr Glu Thr Thr Trp His Cys Leu Gly Thr Cys Ser Met Ala 
                565                 570                 575     


Pro Arg Glu Gly Ser Lys Val Val Pro Thr Gly Gly Val Val Asp Ser 
            580                 585                 590         


Arg Leu Asn Val Tyr Gly Val Glu Lys Leu Lys Val Ala Asp Leu Ser 
        595                 600                 605             


Ile Cys Pro Asp Asn Val Gly Cys Asn Thr Tyr Ser Thr Ala Leu Leu 
    610                 615                 620                 


Ile Gly Glu Lys Ala Ser Thr Leu Val Ala Glu Asp Leu Gly Tyr Ser 
625                 630                 635                 640 


Gly Asp Ala Leu Lys Met Thr Val Pro Asn Phe Lys Leu Gly Thr Tyr 
                645                 650                 655     


Glu Glu Ala Gly Leu Ala Arg Phe 
            660                 


<210>  14
<211>  1995
<212>  DNA
<213>  Ogataea methanolica

<400>  14
atggctattc cagatgaatt tgatattatt gttgtcggtg gtggttccac cggttgtgct       60

cttgctggta gattaggtaa cttggacgaa aacgtcacag ttgctttaat cgaaggtggt      120

gaaaacaaca tcaacaaccc atgggtttac ttaccaggtg tttatccaag aaacatgaga      180

ttagactcaa agactgctac tttttactct tcaagaccat caccacactt gaacggtaga      240

agagctattg ttccatgtgc taacatcttg ggtggtggtt cttccatcaa cttcttgatg      300

tacaccagag cctctgcctc cgattacgat gattgggaat ctgaaggttg gactaccgat      360

gaattattac cactaatgaa gaagattgaa acttatcaaa gaccatgtaa caacagagaa      420

ttgcacggtt tcgatggtcc aattaaggtt tcatttggta actatactta tccaaacggt      480

caagatttca ttagagctgc cgaatctcaa ggtattccat ttgttgatga tgctgaagat      540

ttgaaatgtt cccacggtgc tgagcactgg ttgaagtgga tcaacagaga cttaggtaga      600

agatccgatt ctgctcatgc ttacattcac ccaaccatga gaaacaagca aaacttgttc      660

ttgattactt ccaccaagtg tgaaaagatt atcattgaaa acggtgttgc tactggtgtt      720

aagactgttc caatgaagcc aactggttct ccaaagaccc aagttgctag aactttcaag      780

gctagaaagc aaattattgt ttcttgtggt actatctcat caccattagt tttgcaaaga      840

tctggtatcg gttccgctca caagttgaga caagttggta ttaaaccaat tgttgactta      900

ccaggtgttg gtatgaactt ccaagatcac tactgtttct tcactccata ccatgtcaag      960

ccagatactc catcattcga tgactttgtt agaggtgata aagctgttca aaaatctgct     1020

ttcgaccaat ggtatgctaa caaggatggt ccattaacca ctaatggtat tgaggcaggt     1080

gttaagatta gaccaactga agaagaatta gccactgctg atgacgaatt cagagctgct     1140

tatgatgact actttggtaa caagccagat aagccattaa tgcactactc tctaatttct     1200

ggtttctttg gtgaccacac caagattcca aacggtaagt acatgtgcat gttccacttc     1260

ttggaatatc cattctccag aggtttcgtt cacgttgttt ctccaaaccc atacgatgct     1320

cctgactttg atccaggttt catgaacgat ccaagagata tgtggccaat ggtttggtct     1380

tacaagaagt ccagagaaac tgccagaaga atggactgtt ttgccggtga agttacttct     1440

caccacccac actacccata cgactcacca gccagagctg ctgacatgga cttggaaact     1500

actaaagctt atgctggtcc agaccacttt actgctaact tgtaccacgg ttcatggact     1560

gttccaattg aaaagccaac tccaaagaac gctgctcacg ttacttctaa ccaagttgaa     1620

aaacatcgtg acatcgaata caccaaggag gatgatgctg ctatcgaaga ttacatcaga     1680

gaacacactg aaaccacatg gcattgtctt ggtacttgtt caatggctcc aagagaaggt     1740

tctaaggttg tcccaactgg tggtgttgtt gactccagat taaacgttta cggtgttgaa     1800

aagttgaagg ttgctgattt atcaatttgc ccagataatg ttggttgtaa cacttactct     1860

actgctttgt taatcggtga aaaggcttct accttagttg ctgaagactt gggctactct     1920

ggtgatgctt tgaagatgac tgttccaaac ttcaaattgg gtacttatga agaagctggt     1980

ctagctagat tctag                                                      1995


<210>  15
<211>  663
<212>  PRT
<213>  Ogataea methanolica

<400>  15

Met Ala Ile Pro Glu Glu Phe Asp Ile Ile Val Val Gly Gly Gly Ser 
1               5                   10                  15      


Ala Gly Cys Pro Thr Ala Gly Arg Leu Ala Asn Leu Asp Pro Asn Leu 
            20                  25                  30          


Thr Val Ala Leu Ile Glu Ala Gly Glu Asn Asn Ile Asn Asn Pro Trp 
        35                  40                  45              


Val Tyr Leu Pro Gly Val Tyr Pro Arg Asn Met Arg Leu Asp Ser Lys 
    50                  55                  60                  


Thr Ala Thr Phe Tyr Ser Ser Arg Pro Ser Pro His Leu Asn Gly Arg 
65                  70                  75                  80  


Arg Ala Ile Val Pro Cys Ala Asn Ile Leu Gly Gly Gly Ser Ser Ile 
                85                  90                  95      


Asn Phe Met Met Tyr Thr Arg Gly Ser Ala Ser Asp Tyr Asp Asp Trp 
            100                 105                 110         


Glu Ser Glu Gly Trp Thr Thr Asp Glu Leu Leu Pro Leu Met Lys Arg 
        115                 120                 125             


Leu Glu Thr Tyr Gln Arg Pro Cys Asn Asn Pro Asp Leu His Gly Phe 
    130                 135                 140                 


Asp Gly Pro Ile Lys Val Ser Phe Gly Asn Tyr Thr Tyr Pro Asn Cys 
145                 150                 155                 160 


Gln Asp Phe Leu Arg Ala Ala Glu Ser Gln Gly Ile Pro Phe Val Asp 
                165                 170                 175     


Asp Ala Glu Asp Leu Lys Thr Ser His Ala Ser Gln His Trp Leu Lys 
            180                 185                 190         


Trp Ile Asn Arg Asp Leu Gly Arg Arg Ser Asp Ala Ala His Ala Tyr 
        195                 200                 205             


Ile His Pro Thr Met Arg Asn Lys Ser Asn Leu Tyr Leu Ile Thr Ser 
    210                 215                 220                 


Thr Lys Ala Asp Lys Val Ile Ile Glu Asp Gly Val Ala Ala Gly Ile 
225                 230                 235                 240 


Gln Val Val Pro Ser Lys Pro Leu Asn Pro Glu Lys Pro Ala Ala Lys 
                245                 250                 255     


Ile Tyr Lys Ala Arg Lys Gln Ile Ile Leu Ser Cys Gly Thr Ile Ser 
            260                 265                 270         


Thr Pro Leu Val Leu Gln Arg Ser Gly Ile Gly Ser Ala His Lys Leu 
        275                 280                 285             


Arg Gln Ala Gly Ile Lys Pro Ile Val Asp Leu Pro Gly Val Gly Met 
    290                 295                 300                 


Asn Phe Gln Asp His Tyr Cys Phe Phe Thr Pro Tyr His Val Lys Pro 
305                 310                 315                 320 


Asp Thr Pro Ser Phe Asp Asp Phe Ala Arg Gly Asp Lys Ala Val Gln 
                325                 330                 335     


Lys Ser Ala Phe Asp Gln Trp Tyr Ala Asn Lys Asp Gly Pro Leu Thr 
            340                 345                 350         


Thr Asn Gly Ile Glu Ala Gly Val Lys Ile Arg Pro Thr Ala Glu Glu 
        355                 360                 365             


Leu Ala Thr Ala Asp Glu Asp Phe Gln Leu Gly Tyr Ala Ser Tyr Phe 
    370                 375                 380                 


Glu Asn Lys Pro Asp Lys Pro Leu Met His Tyr Ser Leu Ile Ser Gly 
385                 390                 395                 400 


Phe Phe Gly Asp His Thr Lys Ile Pro Asn Gly Lys Tyr Met Thr Met 
                405                 410                 415     


Phe His Phe Leu Glu Tyr Pro Phe Ser Arg Gly Phe Val His Val Val 
            420                 425                 430         


Ser Pro Ser Pro Tyr Asp Ala Pro Asp Phe Asp Pro Gly Phe Met Asn 
        435                 440                 445             


Asp Pro Lys Asp Met Trp Pro Met Val Trp Ala Tyr Lys Met Ser Arg 
    450                 455                 460                 


Glu Thr Ala Arg Arg Met Glu Cys Phe Ala Gly Glu Val Thr Ser His 
465                 470                 475                 480 


His Pro Lys Tyr Pro Tyr Asp Ser Pro Ala Arg Ala Lys Asp Leu Asp 
                485                 490                 495     


Leu Glu Thr Cys Lys Ala Tyr Ala Gly Pro Asp His Phe Thr Ala Asn 
            500                 505                 510         


Leu Tyr His Gly Ser Trp Thr Ile Pro Leu Glu Lys Pro Thr Pro Lys 
        515                 520                 525             


Asn Thr Ser His Val Thr Ser Asn Gln Val Glu Leu His Ala Gln Leu 
    530                 535                 540                 


Glu Tyr Ser Lys Glu Asp Asp Ile Ala Ile Glu Asn Tyr Ile Lys Glu 
545                 550                 555                 560 


His Val Glu Thr Thr Trp His Cys Leu Gly Thr Cys Ser Met Ala Pro 
                565                 570                 575     


Arg Glu Gly Ser Ser Ile Val Pro Thr Gly Gly Val Val Asp Glu Arg 
            580                 585                 590         


Leu Asn Val Tyr Asp Val Ala His Leu Lys Cys Ala Asp Leu Ser Ile 
        595                 600                 605             


Cys Pro Asp Asn Val Gly Cys Asn Thr Tyr Ser Thr Ala Leu Leu Val 
    610                 615                 620                 


Gly Glu Lys Ala Ser Met Ile Val Ala Glu Asp Leu Gly Tyr Ser Gly 
625                 630                 635                 640 


Ala Glu Leu Asp Met Thr Ile Pro Gly Phe Lys Leu Gly Thr Tyr Glu 
                645                 650                 655     


Ser Thr Gly Leu Gly Arg Phe 
            660             


<210>  16
<211>  1992
<212>  DNA
<213>  Ogataea methanolica

<400>  16
atggctattc ctgaagaatt cgatatcatt gttgtcggtg gtggttctgc cggctgtcct       60

actgctggta gattggctaa cttagaccca aatttaactg ttgctttaat cgaagctggt      120

gaaaacaaca ttaacaaccc atgggtctac ttaccaggcg tttacccaag aaacatgaga      180

ttagactcca aaactgcaac tttctactct tctagacctt ccccacattt aaatggtaga      240

agagctattg ttccatgtgc taatatctta ggtggtggtt cttcaattaa cttcatgatg      300

tacactagag gttcagcttc tgattatgat gactgggaat ccgaaggttg gactaccgat      360

gaattattgc cattgatgaa aagattagaa acttatcaaa gaccatgtaa caaccctgat      420

ttgcacggtt tcgacggccc tatcaaggtc tccttcggta actacactta tcctaactgt      480

caagatttct taagagccgc tgaatctcaa ggtattccat ttgttgatga tgctgaagat      540

ttaaagactt ctcatgcttc ccaacactgg ctgaagtgga ttaacagaga cctgggtaga      600

agatctgatg ctgcgcatgc ttacattcac ccaactatga gaaacaagtc aaacttatac      660

ttgatcactt ccactaaggc tgataaagtt ataattgaag atggagttgc agctggtatt      720

caagttgttc cttccaaacc attgaaccca gaaaagccgg ctgccaagat ctacaaggct      780

agaaagcaaa tcattctatc ctgtggtaca atttctaccc cgttggtcct acaaagatct      840

ggtattggct cagctcataa attaagacag gcaggcataa aaccgatcgt tgacttgcca      900

ggagttggta tgaacttcca agatcactac tgctttttca ccccatacca tgtcaagcca      960

gatactcctt cttttgatga ctttgccaga ggtgataagg ctgttcaaaa atcagctttt     1020

gatcaatggt atgctaacaa agatggtcct ttaaccacta acggtattga agctggtgtt     1080

aagattagac caactgctga agaactggct actgctgatg aagatttcca actaggctac     1140

gcttcttact ttgaaaacaa gccagataaa ccattgatgc attactcttt aatctctggt     1200

ttctttggtg atcacactaa gattccaaac ggtaaataca tgaccatgtt ccatttctta     1260

gaatacccat tctccagggg ttttgttcac gttgtttcgc caagcccata cgatgctcca     1320

gactttgacc caggtttcat gaacgaccca aaggacatgt ggccaatggt ttgggcttat     1380

aaaatgtcaa gagaaactgc tagaagaatg gaatgctttg ctggtgaagt tacttcccac     1440

catcctaaat atccatacga ttcacctgcc agagctaagg acttggactt ggaaacttgt     1500

aaagcttacg cgggtccaga tcactttact gcaaacttgt accacggttc gtggaccatt     1560

ccattggaga agccaactcc caagaacact tctcacgtta cttcgaatca agttgaatta     1620

catgctcaat tagaatattc taaagaagat gacatcgcca tcgaaaacta tatcaaggaa     1680

cacgttgaaa ctacctggca ttgtcttggt acttgttcaa tggctccaag agaaggctca     1740

tcaattgtcc caacaggtgg tgttgttgat gaaagactaa acgtttatga tgttgctcac     1800

ttaaaatgtg ctgatttatc tatctgtcca gataatgtgg gttgtaacac ttactctact     1860

gcgttactgg ttggtgaaaa ggcttctatg attgttgctg aagatttagg ttactctgga     1920

gctgaattgg atatgaccat tcctggtttc aagttaggta cttacgaatc tactggatta     1980

ggtagattct aa                                                         1992


<210>  17
<211>  1000
<212>  DNA
<213>  Ogataea methanolica

<400>  17
gacaaagttt tgttaaatga ctatcgaaca agccatgaaa tagcacattt ctgccagtca       60

cttttaacac tttcctgctt gctggttgac tctcctcata caaacaccca aaagggaaac      120

tttcagtgtg gggacacttg acatctcaca tgcaccccag attaatttcc ccagacgatg      180

cggagacaag acaaaacaac cctttgtcct gctcttttct ttctcacacc gcgtgggtgt      240

gtgcgcaggc aggcaggcag gcagcgggct gcctgccatc tctaatcgct gctcctcccc      300

cctggcttca aataacagcc tgctgctatc tgtgaccaga ttgggacacc cccctcccct      360

ccgaatgatc catcaccttt tgtcgtactc cgacaatgat ccttccctgt catcttctgg      420

caatcagctc cttcaataat taaatcaaat aagcataaat agtaaaatcg catacaaacg      480

tcatgaaaag ttttatctct atggccaacg gatagtctat ctgcttaatt ccatccactt      540

tgggaaccgt tctctcttta ccccagattc tcaaagctaa tatctgcccc ttgtctattg      600

tcctttctcc gtgtacaagc ggagcttttg cctcccatcc tcttgctttg tttcggttat      660

ttttttttct tttgaaactc ttggtcaaat caaatcaaac aaaaccaaac cttctattcc      720

atcagatcaa ccttgttcaa cattctataa atcgatataa atataacctt atccctccct      780

tgttttttac caattaatca atcttcaaat ttcaaatatt ttctacttgc tttattactc      840

agtattaaca tttgtttaaa ccaactataa cttttaactg gctttagaag ttttatttaa      900

catcagtttc aatttacatc tttatttatt aacgaaatct ttacgaatta actcaatcaa      960

aacttttacg aaaaaaaaat cttactatta atttctcaaa                           1000


<210>  18
<211>  1000
<212>  DNA
<213>  Ogataea methanolica

<400>  18
cgaacttgcc cttgtggaat ttggttgtta atcaaactgt tctgtatttc atgtcatact       60

actattgata ttattaatgt tacttactca tctggccatt taacaggttt gaagctttaa      120

tgctcttaac taacagcaat ccatcaccgt caaccttaac ccccctggtg cttgctgtct      180

ttatccttcg tatctttttc atgttgcacc gccctgttcc ttatacggtt gttcccccat      240

aggctaactt ctctgtttcc gaccatctct gcaataacaa agaattctat acgcttacac      300

tataatcata caatgactct acatgccatt ttcactttac ttacttgcca tcggaagata      360

ctgaatcaga aagccatagt aactacataa cttcaaaaca cacccttttt acagattagt      420

tacaattttg tcaatgtttg tttgataacc caaggtggaa cgtttccagt tagacctgtt      480

taatccaact cactttacca ccccaaaact ttcctaccgt tagacaaata ctggctaaat      540

ctgacgaaaa caaccaatca acaattgaat ccactgggag gtatctctaa tccactgaca      600

aactttgcta aaacaagaaa aagtgggggc ctccgttgcg gagaagacgt gcgcaggctt      660

aaaaacacaa gagaacactt ggaagtaccc cagattttta gcttcctact attctgacac      720

cccctattca agcacgacgg tgattgattc attcaatttt gctgctccaa tgataggata      780

aacccttttg gacttcaatc agacctctgt cctccatagc aatataaata ccttctagtt      840

gccccacttc ctctctcctg tactgcccca atgagtgact tattcaagtt actttctctc      900

ttttcctaac aattaaacaa gaagctttat tataacatta atatactatt ttataacagg      960

attgaaaatt atatttatct atctaaaact aaaattcaaa                           1000


<210>  19
<211>  664
<212>  PRT
<213>  Ogataea polymorpha

<400>  19

Met Ala Ile Pro Asp Glu Phe Asp Ile Ile Val Val Gly Gly Gly Ser 
1               5                   10                  15      


Thr Gly Cys Cys Ile Ala Gly Arg Leu Ala Asn Leu Asp Asp Gln Asn 
            20                  25                  30          


Leu Thr Val Ala Leu Ile Glu Gly Gly Glu Asn Asn Ile Asn Asn Pro 
        35                  40                  45              


Trp Val Tyr Leu Pro Gly Val Tyr Pro Arg Asn Met Arg Leu Asp Ser 
    50                  55                  60                  


Lys Thr Ala Thr Phe Tyr Ser Ser Arg Pro Ser Lys Ala Leu Asn Gly 
65                  70                  75                  80  


Arg Arg Ala Ile Val Pro Cys Ala Asn Ile Leu Gly Gly Gly Ser Ser 
                85                  90                  95      


Ile Asn Phe Leu Met Tyr Thr Arg Ala Ser Ala Ser Asp Tyr Asp Asp 
            100                 105                 110         


Trp Glu Ser Glu Gly Trp Ser Thr Asp Glu Leu Leu Pro Leu Ile Lys 
        115                 120                 125             


Lys Ile Glu Thr Tyr Gln Arg Pro Cys Asn Asn Arg Asp Leu His Gly 
    130                 135                 140                 


Phe Asp Gly Pro Ile Lys Val Ser Phe Gly Asn Tyr Thr Tyr Pro Thr 
145                 150                 155                 160 


Cys Gln Asp Phe Leu Arg Ala Ala Glu Ser Gln Gly Ile Pro Val Val 
                165                 170                 175     


Asp Asp Leu Glu Asp Phe Lys Thr Ser His Gly Ala Glu His Trp Leu 
            180                 185                 190         


Lys Trp Ile Asn Arg Asp Leu Gly Arg Arg Ser Asp Ser Ala His Ala 
        195                 200                 205             


Tyr Val His Pro Thr Met Arg Asn Lys Gln Ser Leu Phe Leu Ile Thr 
    210                 215                 220                 


Ser Thr Lys Cys Asp Lys Val Ile Ile Glu Asp Gly Lys Ala Val Ala 
225                 230                 235                 240 


Val Arg Thr Val Pro Met Lys Pro Leu Asn Pro Lys Lys Pro Val Ser 
                245                 250                 255     


Arg Thr Phe Arg Ala Arg Lys Gln Ile Val Ile Ser Cys Gly Thr Ile 
            260                 265                 270         


Ser Ser Pro Leu Val Leu Gln Arg Ser Gly Ile Gly Ala Ala His His 
        275                 280                 285             


Leu Arg Ser Val Gly Val Lys Pro Ile Val Asp Leu Pro Gly Val Gly 
    290                 295                 300                 


Glu Asn Phe Gln Asp His Tyr Cys Phe Phe Thr Pro Tyr Tyr Val Lys 
305                 310                 315                 320 


Pro Asp Val Pro Thr Phe Asp Asp Phe Val Arg Gly Asp Pro Val Ala 
                325                 330                 335     


Gln Lys Ala Ala Phe Asp Gln Trp Tyr Ser Asn Lys Asp Gly Pro Leu 
            340                 345                 350         


Thr Thr Asn Gly Ile Glu Ala Gly Val Lys Ile Arg Pro Thr Glu Glu 
        355                 360                 365             


Glu Leu Ala Thr Ala Asp Glu Asp Phe Arg Arg Gly Tyr Ala Glu Tyr 
    370                 375                 380                 


Phe Glu Asn Lys Pro Asp Lys Pro Leu Met His Tyr Ser Val Ile Ser 
385                 390                 395                 400 


Gly Phe Phe Gly Asp His Thr Lys Ile Pro Asn Gly Lys Phe Met Thr 
                405                 410                 415     


Met Phe His Phe Leu Glu Tyr Pro Phe Ser Arg Gly Phe Val Arg Ile 
            420                 425                 430         


Thr Ser Ala Asn Pro Tyr Asp Ala Pro Asp Phe Asp Pro Gly Phe Leu 
        435                 440                 445             


Asn Asp Glu Arg Asp Leu Trp Pro Met Val Trp Ala Tyr Lys Lys Ser 
    450                 455                 460                 


Arg Glu Thr Ala Arg Arg Met Glu Ser Phe Ala Gly Glu Val Thr Ser 
465                 470                 475                 480 


His His Pro Leu Phe Lys Val Asp Ser Pro Ala Arg Ala Arg Asp Leu 
                485                 490                 495     


Asp Leu Glu Thr Cys Ser Ala Tyr Ala Gly Pro Lys His Leu Thr Ala 
            500                 505                 510         


Asn Leu Tyr His Gly Ser Trp Thr Val Pro Ile Asp Lys Pro Thr Pro 
        515                 520                 525             


Lys Asn Asp Phe His Val Thr Ser Asn Gln Val Gln Leu His Ser Asp 
    530                 535                 540                 


Ile Glu Tyr Thr Glu Glu Asp Asp Glu Ala Ile Val Asn Tyr Ile Lys 
545                 550                 555                 560 


Glu His Thr Glu Thr Thr Trp His Cys Leu Gly Thr Cys Ser Met Ala 
                565                 570                 575     


Pro Arg Glu Gly Ser Lys Ile Ala Pro Lys Gly Gly Val Leu Asp Ala 
            580                 585                 590         


Arg Leu Asn Val Tyr Gly Val Gln Asn Leu Lys Val Ala Asp Leu Ser 
        595                 600                 605             


Val Cys Pro Asp Asn Val Gly Cys Asn Thr Tyr Ser Thr Ala Leu Thr 
    610                 615                 620                 


Ile Gly Glu Lys Ala Ala Thr Leu Val Ala Glu Asp Leu Gly Tyr Ser 
625                 630                 635                 640 


Gly Ser Asp Leu Asp Met Thr Ile Pro Asn Phe Arg Leu Gly Thr Tyr 
                645                 650                 655     


Glu Glu Thr Gly Leu Ala Arg Phe 
            660                 


<210>  20
<211>  1995
<212>  DNA
<213>  Ogataea polymorpha

<400>  20
atggccattc ctgacgaatt cgatatcatt gttgttggtg gaggttccac cggctgctgc       60

attgcgggca gactcgcaaa cctcgacgac caaaacctca cagttgccct gatcgagggt      120

ggtgagaaca acatcaacaa cccttgggtc taccttcccg gagtgtatcc tagaaacatg      180

agactcgact ccaagacggc caccttctac tcgtccagac catcgaaggc tctgaacggc      240

agaagagcga tcgttccttg cgccaacatc cttggaggcg gctcgtcgat caactttctg      300

atgtacacca gagcctctgc ttccgactac gacgactggg agtccgaggg atggagcacc      360

gacgagttgc tacctctgat caaaaaaatc gaaacttacc agcgtccttg caacaacaga      420

gatctgcacg gctttgacgg cccaatcaag gtttcctttg gaaactacac gtatcctacg      480

tgccaggact tcctgagagc agcagagtcg cagggaattc ctgttgtgga cgacctggag      540

gacttcaaga catcgcatgg tgcagagcac tggctgaagt ggattaacag agacctgggc      600

agaagatcgg attctgcgca cgcctacgtc cacccaacta tgagaaacaa gcagagcctg      660

ttcctcatca cctccaccaa gtgtgacaag gtgatcatcg aggacggcaa ggctgtggcc      720

gtgagaacag tgccaatgaa gcctctgaac cctaagaagc ctgtgtccag aaccttcaga      780

gccagaaagc agattgtgat ctcctgcgga accatctcgt ctcctctggt gctccagaga      840

tctggtattg gtgcagctca ccacttgaga tccgtggggg tcaagccaat cgtcgacctg      900

ccaggtgtgg gtgagaattt ccaggaccac tactgtttct tcactccata ctacgtcaag      960

cctgacgttc ctacgttcga cgactttgtc aggggtgacc cagttgccca gaaggccgct     1020

ttcgaccagt ggtactccaa caaggacggt ccattgacca ccaacggtat tgaagccgga     1080

gtcaagatca gacctaccga agaggagctg gctaccgcgg acgaggactt cagacgcggc     1140

tacgcagagt acttcgagaa caagccagac aagcctctga tgcactactc tgtcatctcc     1200

ggcttctttg gagaccacac caagattcct aacggcaagt tcatgaccat gttccacttc     1260

ctggagtatc cattctccag aggatttgtt agaatcacct cggcaaaccc atacgacgct     1320

cctgacttcg atcccggctt cctcaatgac gaaagagacc tgtggcctat ggtctgggca     1380

tacaagaagt ccagagagac ggccagaaga atggagagct ttgcaggaga ggtcacctcg     1440

caccacccat tgttcaaggt tgactcgcca gccagagcca gagacctgga cctcgagaca     1500

tgcagtgcat atgccggtcc taagcacctc actgccaacc tgtaccacgg ctcgtggacc     1560

gttcctatcg acaagccaac gcctaagaac gatttccacg tgacctccaa ccaagtccaa     1620

ctgcactccg acatcgagta caccgaggag gacgacgagg ccatcgtcaa ctacattaag     1680

gaacacaccg agaccacttg gcactgtctg ggtacctgct cgatggcccc aagagagggt     1740

agtaagattg ctcctaaggg aggtgtcttg gacgccagac tgaacgttta cggagtccag     1800

aacctcaagg ttgcggacct ttctgtttgt cccgacaacg ttggatgcaa cacctactct     1860

actgcattga ccatcggtga gaaggctgcc actcttgttg ctgaagatct tggctactca     1920

ggctccgacc tggacatgac gattccaaac ttcagactcg gaacttacga ggagaccgga     1980

cttgccagat tctaa                                                      1995


<210>  21
<211>  1000
<212>  DNA
<213>  Ogataea polymorpha

<400>  21
gctgcagctt gcgatctcgg atggttttgg aatggaagaa ccgcgacatc tccaacagct       60

gggccgtgtt gagaatgagc cggacgtcgt tgaacgaggg ggccacaagc cggcgtttgc      120

tgatggcgcg gcgctcgtcc tcgatgtaga aggccttttc cagaggcagt ctcgtgaaga      180

agttgccaac gctcggaacc agctgcacga gccgagacaa ttcgggggtg ccggctttgg      240

tcatttcaat gttgtcgtcg atgaggagtt caaggtcgtg gaagatttcc gcgtagcggc      300

gttttgcctc agagtttacc atgaggtcgt ccactgcaga gatgccgttg ctcttcaccg      360

cgtacaggac gaacggcgtg gccagcaggc ccttgatcca ttctatgagg ccatctcgac      420

ggtgttcctt gagtgcgtac tccactctgt agcgactgga catctcgaga ctgggcttgc      480

tgtgctggat gcaccaatta attgttgccg catgcatcct tgcaccgcaa gtttttaaaa      540

cccactcgct ttagccgtcg cgtaaaactt gtgaatctgg caactgaggg ggttctgcag      600

ccgcaaccga acttttcgct tcgaggacgc agctggatgg tgtcatgtga ggctctgttt      660

gctggcgtag cctacaacgt gaccttgcct aaccggacgg cgctacccac tgctgtctgt      720

gcctgctacc agaaaatcac cagagcagca gagggccgat gtggcaactg gtggggtgtc      780

ggacaggctg tttctccaca gtgcaaatgc gggtgaaccg gccagaaagt aaattcttat      840

gctaccgtgc agtgactccg acatccccag tttttgccct acttgatcac agatggggtc      900

agcgctgccg ctaagtgtac ccaaccgtcc ccacacggtc catctataaa tactgctgcc      960

agtgcacggt ggtgacatca atctaaagta caaaaacaaa                           1000


<210>  22
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal sequence


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  X at position 3 is either F or L

<220>
<221>  MISC_FEATURE
<222>  (16)..(16)
<223>  X at position 16 is either A or T

<400>  22

Met Lys Xaa Ser Thr Asn Leu Ile Leu Ala Ile Ala Ala Ala Ser Xaa 
1               5                   10                  15      


Val Val Ser Ala 
            20  


<210>  23
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader sequence


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  X at position 3 is either F or L

<220>
<221>  MISC_FEATURE
<222>  (16)..(16)
<223>  X at position 16 is either A or T

<400>  23

Met Lys Xaa Ser Thr Asn Leu Ile Leu Ala Ile Ala Ala Ala Ser Xaa 
1               5                   10                  15      


Val Val Ser Ala Ala Pro Val Ala Pro Ala Glu Glu Ala Ala Asn His 
            20                  25                  30          


Leu His Lys Arg 
        35      


<210>  24
<211>  85
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  24

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          


Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
        35                  40                  45              


Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50                  55                  60                  


Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  


Ser Leu Glu Lys Arg 
                85  


<210>  25
<211>  1991
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AOX1 split marker cassette 1

<400>  25
aggggtccaa gtaagaagct tcttgctgta gaatttgggc atatgtgctg gtgacaaagg       60

catctctgcc ttgagtttct gacggcggga cagcattctt accggatata taacaccaat      120

tgccagcacc accaatctca gaggtacccc taacaaactt aataaaatct tgggtatcaa      180

cttcattaag ctttgtagtt tgcaagtact tataaacaaa attccgtaag gtgtcgtctt      240

gaggctggga cttgacaaac tgccaaaatg gcaacaaatc tactggcttg gccataattt      300

tgacattcga gtcatcaaag gtaaattcaa ccggagactt gtattcttta ttgataactt      360

tctcatatag gacattgtca ggaacacgat gaaaccagga tgcccccaaa tccaatgaga      420

ctgaggtttc atgagtcgca accaacctac ctccaatacg gtccctaccc tctaaaatca      480

acgcattcac gccattgctt ttgagatcga ctgcagcttt gatgcctgaa atcccagcgc      540

ctacaatgat gacatttgga tttggttgac tcatgttggt attgtgaaat agacgcagat      600

cgggaacact gaaaaataac agttattatt cgagatctaa catccaaaga cgaaaggttg      660

aatgaaacct ttttgccatc cgacatccac aggtccattc tcacacataa gtgccaaacg      720

caacaggagg ggatacacta gcagcagacc gttgcaaacg caggacctcc actcctcttc      780

tcctcaacac ccacttttgc catcgaaaaa ccagcccagt tattgggctt gattggagct      840

cgctcattcc aattccttct attaggctac taacaccatg actttattag cctgtctatc      900

ctggcccccc tggcgaggtt catgtttgtt tatttccgaa tgcaacaagc tccgcattac      960

acccgaacat cactccagat gagggctttc tgagtgtggg gtcagtacgc tgcaggtcga     1020

caacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt ctagatcggt     1080

accgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca     1140

tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc     1200

atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc     1260

gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag     1320

agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa     1380

atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccatg ggtaaggaaa     1440

agactcacgt ttcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata     1500

aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc     1560

ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag     1620

atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt     1680

ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggc aaaacagcat     1740

tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt     1800

tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat     1860

ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg     1920

atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aagcttttgc     1980

cattctcacc g                                                          1991


<210>  26
<211>  1853
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AOX1 split marker cassette 2

<400>  26
aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt       60

acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag      120

cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggcaaaaca      180

gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca      240

gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc      300

gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat      360

tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataagctt      420

ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt      480

tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga      540

taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa      600

cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg      660

atgctcgatg agtttttcta atcagtactg acaataaaaa gattcttgtt ttcaagaact      720

tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga      780

tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag      840

taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat actggtacca ttcgagaacc      900

cttaatataa cttcgtataa tgtatgctat acgaagttat taggtgatat cagatccact      960

gccatttgcc tgagagatgc aggcttcatt tttgatactt ttttatttgt aacctatata     1020

gtataggatt ttttttgtca ttttgtttct tctcgtacga gcttgctcct gatcagccta     1080

tctcgcagct gatgaatatc ttgtggtagg ggtttgggaa aatcattcga gtttgatgtt     1140

tttcttggta tttcccactc ctcttcagag tacagaagat taagtgagac gttcgtttgt     1200

gcaagcttca acgatgccaa aagggtataa taagcgtcat ttgcagcatt gtgaagaaaa     1260

ctatgtggca agccaagcct gcgaagaatg tattttaagt ttgactttga tgtattcact     1320

tgattaagcc ataattctcg agtatctatg attggaagta tgggaatggt gatacccgca     1380

ttcttcagtg tcttgaggtc tcctatcaga ttatgcccaa ctaaagcaac cggaggagga     1440

gatttcatgg taaatttctc tgacttttgg tcatcagtag actcgaactg tgagactatc     1500

tcggttatga cagcagaaat gtccttcttg gagacagtaa atgaagtccc accaataaag     1560

aaatccttgt tatcaggaac aaacttcttg tttcgaactt tttcggtgcc ttgaactata     1620

aaatgtagag tggatatgtc gggtaggaat ggagcgggca aatgcttacc ttctggacct     1680

tcaagaggta tgtagggttt gtagatactg atgccaactt cagtgacaac gttgctattt     1740

cgttcaaacc attccgaatc cagagaaatc aaagttgttt gtctactatt gatccaagcc     1800

agtgcggtct tgaaactgac aatagtgtgc tcgtgttttg aggtcatctt tgt            1853


<210>  27
<211>  2038
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AOX2 split marker cassette 1

<400>  27
gtacgggttt actgatttga catatcttgg tactaacgtt accaatggtg ttccaaataa       60

cgcagatgat gagcgtggtt gcattgctgg atttgacaac actggtttcg tgctgggaac      120

ttcatcctcg ttgtttaatc agtttattct gcaactgaat acgagtgatc tttcaggagc      180

aatttaccaa atcattgagc attttctgac tggacttagc gaagacgaag acgacattgc      240

tatctattcc cccaaccctt tctacaaaag tacgtatgca ggagtaggtg ccattgcgga      300

aaatgacacc ctttacttgg ttgatggtgg agaggataac caaaacgtcc ctctgcagcc      360

tctacttcaa aaggagcgtg acgttgatat catctttgcg tttgacaaca gtgcagacac      420

tgacctctct tggccaaacg gttcatcatt agtcaacacc tacatgagac agttttcttc      480

tcaagcaaat ggaacaacgt tcccttatgt acctgatacc accactttcc taaacttgaa      540

tctttcgagt aagccaacct tctttggttg tgatgctaga aatttgacag acattgttga      600

aggcacggat cacattcctc ccctggttgt ttatctggcc aatagacctt tctcgtattg      660

gagtaacact tcaactttca agttagacta ctctgaatcc gagaagagag gaatgatcca      720

aaacggtttt gaagtgtcgt ctcgtttgaa catgactatt gatgaagaat ggcgtacttg      780

tgttggatgt gcaatcattc gtagacagca ggagagatcc aatgcaacac aaacagagca      840

atgtagaaga tgttttgaga attattgttg gaacggtgat attgacactt ccaccgaaga      900

tatccccgtt aattttacca ctactggagc aaccaatgag gagaatgaca actccacttc      960

aatatcatcg gccaattcgg tagcaccttc caaactttgg taccaagcac cattgctgtt     1020

ggtcggcctt gtcgcattct tcatctagta cgtacgctgc aggtcgacaa cccttaatat     1080

aacttcgtat aatgtatgct atacgaagtt attaggtcta gatcggtacc gacatggagg     1140

cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc     1200

gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata cattttgatg     1260

gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg     1320

ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag     1380

gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat     1440

acagttctca catcacatcc gaacataaac aaccatgggt aaggaaaaga ctcacgtttc     1500

gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga     1560

taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga     1620

gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag     1680

actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc     1740

tgatgatgca tggttactca ccactgcgat ccccggcaaa acagcattcc aggtattaga     1800

agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt     1860

gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca     1920

ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa     1980

tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccg       2038


<210>  28
<211>  1944
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AOX2 split marker cassette 2

<400>  28
aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt       60

acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag      120

cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggcaaaaca      180

gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca      240

gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc      300

gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat      360

tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataagctt      420

ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt      480

tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga      540

taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa      600

cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg      660

atgctcgatg agtttttcta atcagtactg acaataaaaa gattcttgtt ttcaagaact      720

tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga      780

tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag      840

taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat actggtacca ttcgagaacc      900

cttaatataa cttcgtataa tgtatgctat acgaagttat taggtgatat cagatccact      960

tcctcttacg gctttctttc ccaaaaaatc attggggaaa tgtgcccctc atcagagtcc     1020

aatgacccat gaataaagtt tcttgtactg tttaagacga tgaattgcaa cgataatccg     1080

agcagtttac ggggtacatc acgtgctttg catatgatct cggagtcgga tcagttccgg     1140

atgtgatgta ttaccccata gtttcaaact ctaatgcagc cgccaagtgc catacaccct     1200

ccatcaatct atgcttaaag tttttcacca tcgttgggtg gtgatgatga ctcgcttagt     1260

ctctgctgtt cgatattaac tttgtaagga tcgcccttgg atggaaaatt gaggggttgt     1320

aacctgaatt tgcaggctac ttacattgga cttttgagaa ggctggacgg ttgatgaaga     1380

gggctgggtg cagaggaatg gaaaaaaatt tagttgagag gactgcttga aattttagga     1440

aatggagtcc tttaagctga caaaacttca aggatgggga ttttcatgta gctttttcat     1500

gccttcgaca agctaaagga aggtaattga ttctggataa atggatattt gatctgcttt     1560

agcagatgtc aaagttctac tagtgatagt ctggtatctc gtagccttca attgggcgta     1620

tcttactcga agtgttatat ttttagctga cgagacgaag aacgagagag tattgacaca     1680

ttcagaggta agacaatatg tcgtattatc aaaataagta tcgaacctct attaggagcc     1740

tactggctca aatgtgcaac cttagtggtg attgtctctg cttcttgatc acaatctgtc     1800

gtgtttgaga gtgccgatgt atgattttta gtaaatgttt ttcagaaaag gcgctaagta     1860

aataaccagt aagtaataaa taacgtaaaa gtgatttgaa tcataaaaga atcaagatag     1920

aggtcaaagc atagataatc cccc                                            1944


<210>  29
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  29
ggctggaaat agatgtaggg ag                                                22


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  30
tcgcatctcc gcaaatttct c                                                 21


<210>  31
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  31
gatcccattc cctatccatg t                                                 21


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  32
ctctcccccc tcgtaatctt                                                   20


<210>  33
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  enhanced green fluorescent protein (eGFP)

<400>  33
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac      480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa      720


<210>  34
<211>  1830
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human serum albumin (HSA) with its native secretion leader

<400>  34
atgaagtggg ttactttcat ctccttgttg ttcttgttct cctcagctta ctccagaggt       60

gttttcagaa gagatgctca caagtccgag gttgctcaca gattcaagga cttgggtgaa      120

gagaacttca aggctttggt tttgatcgct ttcgctcagt acttgcagca gtgtccattc      180

gaggaccacg ttaagttggt taacgaggtt actgagttcg ctaagacttg tgttgctgac      240

gaatccgctg agaactgtga taagtccttg cacactttgt tcggtgacaa gttgtgtact      300

gttgctactt tgagagaaac ttacggtgag atggctgact gttgtgctaa gcaagagcct      360

gagagaaacg agtgtttctt gcaacacaag gacgacaacc caaacttgcc aagattggtt      420

agaccagagg ttgacgttat gtgtactgct ttccacgaca acgaagagac tttcttgaag      480

aagtacttgt acgagatcgc tagaagacac ccatacttct acgctccaga gttgttgttc      540

ttcgctaaga gatacaaggc tgctttcact gagtgttgtc aggctgctga taaggctgct      600

tgtttgttgc caaagttgga cgagttgaga gatgagggta aggcttcttc cgctaagcag      660

agattgaagt gtgcttcctt gcagaagttc ggagagagag cttttaaggc ttgggctgtt      720

gctagattgt cccagagatt cccaaaggct gagttcgctg aggtttccaa gttggttact      780

gacttgacta aggttcacac agagtgttgt cacggtgact tgttggaatg tgctgatgac      840

agagctgact tggctaagta catctgtgag aaccaggatt ccatctcctc caagttgaaa      900

gaatgttgtg agaagccttt gttggagaag tcccactgta tcgctgaggt tgaaaacgac      960

gaaatgccag ctgacttgcc atctttggct gctgacttcg ttgaatccaa ggacgtctgc     1020

aagaactacg ctgaggctaa ggacgttttc ttgggtatgt tcttgtatga gtacgctaga     1080

agacatccag actactccgt tgttttgttg ttgagattgg ctaagactta cgagactact     1140

ttggagaagt gttgtgctgc tgctgaccca catgagtgtt acgctaaggt tttcgacgag     1200

ttcaagccat tggttgagga accacagaac ttgatcaagc agaactgtga gttgttcgag     1260

cagttgggtg agtacaagtt ccagaacgct ttgttggtta gatacactaa gaaggttcca     1320

caggtttcca ctccaacttt ggttgaggtt tccagaaact tgggtaaggt tggttccaag     1380

tgttgtaagc acccagaggc taagagaatg ccatgtgctg aggactactt gtctgttgtt     1440

ttgaaccagt tgtgtgtctt gcacgaaaag acaccagttt ccgacagagt tactaagtgt     1500

tgtactgaat ccttggttaa cagaagacct tgtttctccg ctttggaggt tgacgagact     1560

tacgttccaa aagagttcaa cgctgagact ttcactttcc acgctgacat ctgtactttg     1620

tccgagaaag agagacagat caagaagcag actgctttgg ttgagttggt taagcacaag     1680

ccaaaggcta caaaagagca gttgaaggct gttatggacg acttcgctgc tttcgttgag     1740

aaatgttgta aggctgacga caaagagact tgtttcgctg aagagggtaa gaagttggtt     1800

gctgcttccc aagctgcttt gggtctgtaa                                      1830


<210>  35
<211>  1056
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variable region of a camelid antibody (VHH) with the S. 
       cerevisiae alpha-mating factor leader

<400>  35
atgagattcc catctatttt caccgctgtc ttgttcgctg cctcctctgc attggctgcc       60

cctgttaaca ctaccactga agacgagact gctcaaattc cagctgaagc agttatcggt      120

tactctgacc ttgagggtga tttcgacgtc gctgttttgc ctttctctaa ctccactaac      180

aacggtttgt tgttcattaa caccactatc gcttccattg ctgctaagga agagggtgtc      240

tctctcgaga agagacaagc cggtggttca ttaagattgt cctgtgctgc ctctggtaga      300

actttcactt ctttcgcaat gggttggttt agacaagcac ctggaaaaga gagagagttt      360

gttgcttcta tctccagatc cggtacttta actagatacg ctgactctgc caagggtaga      420

ttcactattt ctgttgacaa cgccaagaac actgtttctt tgcaaatgga caaccttaac      480

ccagatgaca ccgcagtcta ttactgtgcc gctgacttgc acagaccata cggtccagga      540

acccaaagat ccgatgagta cgattcttgg ggtcagggaa ctcaagtcac tgtctcttca      600

ggtggtggat ctggtggtgg aggttcaggt ggtggaggat ccggtggtgg tggttctggt      660

ggtggtggat ctggtggagg tgaagttcaa cttgtcgaat ccggtggtgc acttgtccaa      720

cctggtggat ctcttagact ttcttgtgcc gcctccggtt ttcctgttaa ccgttactct      780

atgcgttggt acagacaagc ccctggaaaa gaacgtgaat gggttgccgg aatgtcctca      840

gctggtgaca gatcctccta cgaagattct gtgaagggac gtttcaccat ctccagagat      900

gacgcccgta acaccgttta ccttcaaatg aactccctta agcctgagga tactgccgtc      960

tactattgta acgtgaatgt cggatttgaa tactggggac agggaaccca agttactgtc     1020

tcttccggtg gacatcacca ccaccatcac taatag                               1056


<210>  36
<211>  991
<212>  DNA
<213>  Pichia pastoris

<400>  36
gcaaggcaac tgagaaattg aatagtggtt tcaagcccgc tgactttttg tattatctca       60

atgtcggtgt ttcacagtcc ccagaagggg gctttgcctt caagggagac ggaagagaca      120

tcgtcaaccc tggggagaag tatttcaaat ggcgcaagtt cgctaatttt tacgattaag      180

cagtgctgta tggggtagtt aataaatcgg gaatatcctt ctgacgtgac tgtaacaaat      240

ctctttttac gtggtgcgca tactggacag aggcagagtc tcaatttctt cttttgagac      300

aggctactac agcctgtgat tcctcttggt acttggattt gcttttatct ggctccgttg      360

ggaactgtgc ctgggttttg aagtatcttg tggatgtgtt tctaacactt tttcaatctt      420

cttggagtga gaatgcagga ctttgaacat cgtctagctc gttggtaggt gaaccgtttt      480

accttgcatg tggttaggag ttttctggag taaccaagac cgtcttatca tcgccgtaaa      540

atcgctctta ctgtcgctaa taatcccgct ggaagagaag ttcgaacaga agtagcacgc      600

aaagctcttg tcaaatgaga attgttaatc gtttgacagg tcacactcgt gggctatgta      660

cgatcaactt gccggctgtt gctggagaga tgacaccagt tgtggcatgg ccaattggta      720

ttcagccgta ccactgtatg gaaaatgaga ttatcttgtt cttgatctag tttcttgcca      780

ttttagagtt gccacattcg taggtttcag taccaataat ggtaacttcc aaacttccaa      840

cgcagatacc agagatctgc cgatccttcc ccaacaatag gagcttacta cgccatacat      900

atagcctatc tattttcact ttcgcgtggg tgcttctata taaacggttc cccatcttcc      960

gtttcatact acttgaattt taagcactaa a                                     991


<210>  37
<211>  864
<212>  DNA
<213>  Pichia pastoris

<400>  37
cttttctttg ggcaaggaaa aatcaagaaa aagcagaggt taaagttttc aggggaatgg       60

caattgcttt atatatggga gaaagttaac tacgtcggtg ctgtaggcgt agagagcgac      120

tggagaatgc gtgatgaggt cgtctctttt cgccccccct tggcggggta aaaattgcac      180

tactgcagaa ttactacacc cctattccga ggagacggag tgcgacaaaa atggtaaagt      240

tcaccctagt ctgcgacttt taattgacgg acaccggcgt ttacatgcga aaaaaactaa      300

agtgcgcgca tttcacggcc gaggggggtc ccacttggga ctgagagggg gtgggatctg      360

aaatcgagga ggtatcaaga ccccccgttt ctcaactccc taatcaaaaa ttacgaagtc      420

ctcgttggaa aggagttaaa ataattaagc ggggtcggac gccataccga ggttatcttg      480

caggcatttt actaatattg gaattcggag ctcaacttgc aaccaggcag ggtttagcta      540

tgtaatcaat gtaatcaata taataaagca ctaccacatc gaaggtttgg gagggaggcc      600

aatagtgtcc cccacagggt gctgatatcg cgattcttgg gtgaggagac acatatttca      660

ctcctctcac caaccaacca agcggctcct cgcaagatga tttatccgat tatccggaca      720

ctatactccc atccagtttg atgccgattt catcgattgt cctaaataat ccttaaatat      780

gtatagaacg gtaccctggg gttacataat ccttatttaa taatccctcc cccaccgctt      840

ttcttttttt ttcttcttat tgtc                                             864


<210>  38
<211>  1000
<212>  DNA
<213>  Pichia pastoris

<400>  38
aaatggcaga aggatcagcc tggacgaagc aaccagttcc aactgctaag taaagaagat       60

gctagacgaa ggagacttca gaggtgaaaa gtttgcaaga agagagctgc gggaaataaa      120

ttttcaattt aaggacttga gtgcgtccat attcgtgtac gtgtccaact gttttccatt      180

acctaagaaa aacataaaga ttaaaaagat aaacccaatc gggaaacttt agcgtgccgt      240

ttcggattcc gaaaaacttt tggagcgcca gatgactatg gaaagaggag tgtaccaaaa      300

tggcaagtcg ggggctactc accggatagc caatacattc tctaggaacc agggatgaat      360

ccaggttttt gttgtcacgg taggtcaagc attcacttct taggaatatc tcgttgaaag      420

ctacttgaaa tcccattggg tgcggaacca gcttctaatt aaatagttcg atgatgttct      480

ctaagtggga ctctacggct caaacttcta cacagcatca tcttagtagt cccttcccaa      540

aacaccattc taggtttcgg aacgtaacga aacaatgttc ctctcttcac attgggccgt      600

tactctagcc ttccgaagaa ccaataaaag ggaccggctg aaacgggtgt ggaaactcct      660

gtccagttta tggcaaaggc tacagaaatc ccaatcttgt cgggatgttg ctcctcccaa      720

acgccatatt gtactgcagt tggtgcgcat tttagggaaa atttacccca gatgtcctga      780

ttttcgaggg ctacccccaa ctccctgtgc ttatacttag tctaattcta ttcagtgtgc      840

tgacctacac gtaatgatgt cgtaacccag ttaaatggcc gaaaaactat ttaagtaagt      900

ttatttctcc tccagatgag actctccttc ttttctccgc tagttatcaa actataaacc      960

tattttacct caaatacctc caacatcacc cacttaaaca                           1000


<210>  39
<211>  549
<212>  DNA
<213>  Pichia pastoris

<400>  39
aatgatataa acaacaattg agtgacaggt ctactttgtt ctcaaaaggc cataaccatc       60

tgtttgcatc tcttatcacc acaccatcct cctcatctgg ccttcaattg tggggaacaa      120

ctagcatccc aacaccagac taactccacc cagatgaaac cagttgtcgc ttaccagtca      180

atgaatgttg agctaacgtt ccttgaaact cgaatgatcc cagccttgct gcgtatcatc      240

cctccgctat tccgccgctt gctccaacca tgtttccgcc tttttcgaac aagttcaaat      300

acctatcttt ggcaggactt ttcctcctgc cttttttagc ctcaggtctc ggttagcctc      360

taggcaaatt ctggtcttca tacctatatc aacttttcat cagatagcct ttgggttcaa      420

aaaagaacta aagcaggatg cctgatatat aaatcccaga tgatctgctt ttgaaactat      480

tttcagtatc ttgattcgtt tacttacaaa caactattgt tgattttatc tggagaataa      540

tcgaacaaa                                                              549


<210>  40
<211>  1000
<212>  DNA
<213>  Pichia pastoris

<400>  40
attactgttt tgggcaatcc tgttgataag acgcattcta gagttgtttc atgaaagggt       60

tacgggtgtt gattggtttg agatatgcca gaggacagat caatctgtgg tttgctaaac      120

tggaagtctg gtaaggactc tagcaagtcc gttactcaaa aagtcatacc aagtaagatt      180

acgtaacacc tgggcatgac tttctaagtt agcaagtcac caagagggtc ctatttaacg      240

tttggcggta tctgaaacac aagacttgcc tatcccatag tacatcatat tacctgtcaa      300

gctatgctac cccacagaaa taccccaaaa gttgaagtga aaaaatgaaa attactggta      360

acttcacccc ataacaaact taataatttc tgtagccaat gaaagtaaac cccattcaat      420

gttccgagat ttagtatact tgcccctata agaaacgaag gatttcagct tccttacccc      480

atgaacagaa atcttccatt taccccccac tggagagatc cgcccaaacg aacagataat      540

agaaaaaaga aattcggaca aatagaacac tttctcagcc aattaaagtc attccatgca      600

ctccctttag ctgccgttcc atccctttgt tgagcaacac catcgttagc cagtacgaaa      660

gaggaaactt aaccgatacc ttggagaaat ctaaggcgcg aatgagttta gcctagatat      720

ccttagtgaa gggttgttcc gatacttctc cacattcagt catagatggg cagctttgtt      780

atcatgaaga gacggaaacg ggcattaagg gttaaccgcc aaattatata aagacaacat      840

gtccccagtt taaagttttt ctttcctatt cttgtatcct gagtgaccgt tgtgtttaat      900

ataacaagtt cgttttaact taagaccaaa accagttaca acaaattata acccctctaa      960

acactaaagt tcactcttat caaactatca aacatcaaaa                           1000


<210>  41
<211>  1000
<212>  DNA
<213>  Pichia pastoris

<400>  41
gtcaactgcg tactcttttg tcgaatggac tactgaatct gcctcgatag ccactatagg       60

aaggtccata gaggccagtt tttcaactag tcttggtgga aagaaaccga caaagccttt      120

catggagtca ccgatactga aaggttcaaa caaagaatgc ttgggtagtc tcttaatacc      180

catggcaacg aaaaaggggt cttcattgtt caacatgaat tcgtatccac ctttaatgta      240

gtcataaagc tgctgaagtt ccgaatcagt gatggaactg tctacagtga caatatagga      300

gttctcaatc accttatatc cagtcgaata tatctggata gggtcgggtc tcactgtgga      360

agattcaaat gggttagatc cctgtaattt cagcgatgga gactcagtat gatggggcaa      420

ggaaaacggc aattggatat tcaattggtc aagagatggt atcaaaagcg agtgtgccag      480

ggtagccacg gtagccactg atgctaatct gataattttc atttctggag tgtcaaaaca      540

gtagtgataa aaggctatga aggaggttgt ctaggggctc gcggaggaaa gtgattcaaa      600

cagacctgcc aaaaagagaa aaaagaggga atccctgttc tttccaatgg aaatgacgta      660

actttaactt gaaaaatacc ccaaccagaa gggttcaaac tcaacaagga ttgcgtaatt      720

cctacaagta gcttagagct gggggagaga caactgaagg cagcttaacg ataacgcggg      780

gggattggtg cacgactcga aaggaggtat cttagtcttg taacctcttt tttccagagg      840

ctattcaaga ttcataggcg atatcgatgt ggagaagggt gaacaatata aaaggctgga      900

gagatgtcaa tgaagcagct ggatagattt caaattttct agatttcaga gtaatcgcac      960

aaaacgaagg aatcccacca agcaaaaaaa aaaatctaag                           1000


<210>  42
<211>  704
<212>  DNA
<213>  Pichia pastoris

<400>  42
aaattaatcc ataagataag gcaaatgtgc ttaagtaatt gaaaacagtg ttgtgattat       60

ataagcatgg tatttgaata gaactactgg ggttaactta tctagtagga tggaagttga      120

gggagatcaa gatgcttaaa gaaaaggatt ggccaatatg aaagccataa ttagcaatac      180

ttatttaatc agataattgt ggggcattgt gacttgactt ttaccaggac ttcaaacctc      240

aaccatttaa acagttatag aagacgtacc gtcacttttg cttttaatgt gatctaaatg      300

tgatcacatg aactcaaact aaaatgatat cttttactgg acaaaaatgt tatcctgcaa      360

acagaaagct ttcttctatt ctaagaagaa catttacatt ggtgggaaac ctgaaaacag      420

aaaataaata ctccccagtg accctatgag caggattttt gcatccctat tgtaggcctt      480

tcaaactcac acctaatatt tcccgccact cacactatca atgatcactt cccagttctc      540

ttcttcccct attcgtacca tgcaaccctt acacgccttt tccatttcgg ttcggatgcg      600

acttccagtc tgtggggtac gtagcctatt ctcttagccg gtatttaaac atacaaattc      660

acccaaattc taccttgata aggtaattga ttaatttcat aaat                       704


<210>  43
<211>  1000
<212>  DNA
<213>  Pichia pastoris

<400>  43
agctcagatt ggaaatgatt tttgatccta ccaagaagcc tttgatttcc agaatctccg       60

ctaagtaagt aacccccgca aacgcatgca tccatgcaaa caaaatacta acaattttag      120

ccccgttgtt gagaaaccca gaaaattgaa tgttcaacca atccagacga tcaataagaa      180

aaaaggccca aaggctactt ccaaacctgc tgccgccaaa cctgctcctt caaaagccgg      240

tcccaaggga ggtaagaagg tgagaaagcc aaagaagaca gttgaagaat tggatcagga      300

aatggctgac tactttgaaa ataagaatta gcccaacaaa atatgtacaa gtattatata      360

aatgaatcta catggtgtgt tttatttaga tcctccaaac caaggaaaga aactaaactt      420

atctccggac ttacgagtca aataactatc cgcagttcct tggaactcag actttcttcc      480

ataagcggtc atatcatctt tggactgtgg gaatcctgga cgaatctttg aaatgtcata      540

atcttgctct ctatctccaa gcacagcgtc cggtaaatgc tggttcttct ttctcagatg      600

aatcttggat ttaacaaata aagccgtgcc tatggctaat gtactcaaaa acaaagtctg      660

cttccagaat ttcgcaaacg atggaatgcc atttcctgta aatgtactca ttgaacctat      720

gtttgattaa agttggtgtg aagtcatcaa acgagagtaa aatcagatac tcgtgcaccg      780

gccaaaattg actgagctaa tctctgcagg cttgacatcc gaacacaaca aataggcgac      840

aaatcttaac tatctaatcg taggctatgg tagaactttg tgggggtaga ggaagactac      900

aacagcaaga caaaacaaaa gagtcatagt ttgactctct gcttttttct tctttctctt      960

ctttttcttc ctccatattc gttatttatt tcgaactgga                           1000


<210>  44
<211>  1000
<212>  DNA
<213>  Pichia pastoris

<400>  44
cagccattaa tctcacctca gtttttgaat cagtagaatt tttaatgaaa caaacggttg       60

gtatattatt tgatagagtt gccaaatttc caaagataaa tttttcatca ggtaatatcc      120

tgaataccgt aacatagtga ctattggaag acactgctat catattatat ttcggataaa      180

aatccaaacc ccagaccgac ctcttgagtc tcaactccaa gtcagccgca actttaatta      240

tccgtggatt gggagctagt ttggacaacg catcagtata atataacttt acggttccat      300

tatcagacgc tattgcaaga acttcctttc cattgatctc gccaatgcgg cagtaattga      360

tatcgtaggg taggtctgga aagacgctgg cgcttgtgtc ccattctgca ggaatctctg      420

gcacggtgct aatggtagtt atccaacgga gctgaggtag tcgatatatc tggatatgcc      480

gcctatagga taaaaacagg agagggtgaa ccttgcttat ggctactaga ttgttcttgt      540

actctgaatt ctcattatgg gaaactaaac taatctcatc tgtgtgttgc agtactattg      600

aatcgttgta gtatctacct ggagggcatt ccatgaatta gtgagataac agagttgggt      660

aactagagag aataatagac gtatgcatga ttactacaca acggatgtcg cactctttcc      720

ttagttaaaa ctatcatcca atcacaagat gcgggctgga aagacttgct cccgaaggat      780

aatcttctgc ttctatctcc cttcctcata tggtttcgca gggctcatgc cccttcttcc      840

ttcgaactgc ccgatgagga agtccttagc ctatcaaaga attcgggacc atcatcgatt      900

tttagagcct tacctgatcg caatcaggat ttcactactc atataaatac atcgctcaaa      960

gctccaactt tgcttgttca tacaattctt gatattcaca                           1000


<210>  45
<211>  1000
<212>  DNA
<213>  Pichia pastoris

<400>  45
tggttccctc tcggtccaat accaaaaata ttatcaccat acaggtctcc cttcgatacc       60

agtgcaaagt tgaaccgtgg gattaccttg gaatctacaa aaatagtgtc actcacaagt      120

ttgtcatcaa ccacgctgcc gcttgcaaag gagaactgaa catgaaggtt gttagggttt      180

gttatattgg aataagtggt ggatttgttg aaggcgaacg caccaaagct acatccgtcc      240

tgagcacact gtgaatttgt cacggaattg accaagaggt cagacgatcc tgtatcccat      300

tgagccgtta tgctttgtgg gggaaaccct atttctatcg tactaagaaa accaatggtg      360

aactcatatt cggtatcaat ggcgacgatt ccagcatagc ctgtagacag taacaacact      420

agggcaacag caactaacat atcttcattg atgaaacgtt gtgatcggtg tgacttttat      480

agtaaaagct acaactgttt gaaataccaa gatatcattg tgaatggctc aaaagggtaa      540

tacatctgaa aaacctgaag tgtggaaaat tccgatggag ccaactcatg ataacgcaga      600

agtcccattt tgccatcttc tcttggtatg aaacggtaga aaatgatccg agtatgccaa      660

ttgatactct tgattcatgc cctatagttt gcgtagggtt taattgatct cctggtctat      720

cgatctggga cgcaatgtag accccattag tggaaacact gaaagggatc caacactcta      780

ggcggacccg ctcacagtca tttcaggaca atcaccacag gaatcaacta cttctcccag      840

tcttccttgc gtgaagcttc aagcctacaa cataacactt cttacttaat ctttgattct      900

cgaattgttt acccaatctt gacaacttag cctaagcaat actctggggt tatatatagc      960

aattgctctt cctcgctgta gcgttcattc catctttcta                           1000


<210>  46
<211>  1001
<212>  DNA
<213>  Pichia pastoris

<400>  46
gatatcgatc tacacttaat agtagatgac gaggcatctc tccaataggt accatatctg       60

gtgtttcttg taatttaaga atctgttggt ctatgaatgt agatttgtca tgaacaatga      120

tatatgggtc aggaggacaa gatggtttct ctgagttggg ttgttgaggt gcctggcaag      180

acttcggagc gttgatatcc ccaagacttg tagtgaccga tagttgaagc gtgtgtttgc      240

aggaacggca catcaatgca actttcgtaa ctttggaatt gagagttgat gcactgatga      300

cgatacccga aattttgacg attttaccaa tatgacttga agacaagtct ctcattgaaa      360

ccttattatc gttactaagc aaaacgagct gacaagaagg gaaggtggtc ggtatttcct      420

cgttgttcaa atatatgatt ctcctggcaa tatctgtgat ggcctgttca aaaagtggaa      480

tcatttctgc aggatcatct accaactttt tattgagctc ctcattgaat acgattaagt      540

ggtcattttg aatcgtcagt aagtacttgt ttacaagtaa attctgtctg agttgttctc      600

tgtagatgta ctgattttcc atacgaaact ccaaaatgaa cgaacggaat gccttaatga      660

cctcactgaa ctggtcatcg ttctgttctc cgggaaggac acttgtgtta aagactgatg      720

ctctatcaaa ggacattgca acaaagtata aacggttgtg agcgggaaaa agatgtgtag      780

gtaattgtcg tagatgagac tgattcagta gaaaacgcgt cctgcactat ttttttcttt      840

cttcattaca tttcctaatc gggacaaaat gaatctaaag acgtggttat gtagtacacg      900

catcgatagg ctatccccat accaaaacac tattttaccc catccttgac aggttataaa      960

tatgcgatag tatgagtatc ttcaaattca gctgaaatat c                         1001


<210>  47
<211>  1000
<212>  DNA
<213>  Pichia pastoris

<400>  47
aataaaaaaa cgttatagaa agaaattgga ctacgatatg ctccaatcca aattgtcaaa       60

attgaccacc gaaaaagaac aattggaatt tgacaagagg aacaactcac tagattctca      120

aacggagcgt cacctagagt cagtttccaa gtcaattaca gaaagtttgg aaacagaaga      180

ggagtatcta caattgaatt ccaaacttaa agtcgagctg tccgaattca tgtcgctaag      240

gctttcttac ttggacccca tttttgaaag tttcattaaa gttcagtcaa aaattttcat      300

ggacatttat gacacattaa agagcggact accttatgtt gattctctat ccaaagagga      360

ttatcagtcc aagatcttgg actctagaat agataacatt ctgtcgaaaa tggaagcgct      420

gaaccttcaa gcttacattg atgattagag caatgatata aacaacaatt gagtgacagg      480

tctactttgt tctcaaaagg ccataaccat ctgtttgcat ctcttatcac cacaccatcc      540

tcctcatctg gccttcaatt gtggggaaca actagcatcc caacaccaga ctaactccac      600

ccagatgaaa ccagttgtcg cttaccagtc aatgaatgtt gagctaacgt tccttgaaac      660

tcgaatgatc ccagccttgc tgcgtatcat ccctccgcta ttccgccgct tgctccaacc      720

atgtttccgc ctttttcgaa caagttcaaa tacctatctt tggcaggact tttcctcctg      780

ccttttttag cctcaggtct cggttagcct ctaggcaaat tctggtcttc atacctatat      840

caacttttca tcagatagcc tttgggttca aaaaagaact aaagcaggat gcctgatata      900

taaatcccag atgatctgct tttgaaacta ttttcagtat cttgattcgt ttacttacaa      960

acaactattg ttgattttat ctggagaata atcgaacaaa                           1000


<210>  48
<211>  1000
<212>  DNA
<213>  Pichia pastoris

<400>  48
attgttgtga atactctcct tcatttggat ttcttggact tcggactctc ttgatctctc       60

ttcgaaagtt ttaactctgt tcatgtataa ttttacccgc tgtaggtcgc tcataatacc      120

atgagtatgc acatctttta ctccattaac tttcaggtat gcaaaataca atgaagatag      180

tatatagctc aaagaattta gcattttgca ttgatctaat tgtgacattt tctctatgat      240

atcatctagc ttcttaaact cgagaatctc gtccaacgag gcagaaacat tgtccagtct      300

tacgtcaaga ttattcacga gtttctggac cgtatcaacg ttttccatct taagattaca      360

gtaagtatcg tccttttgaa ctgcaaaggt agaaaagtta atttttgatt tggtagtaca      420

ctatgaaact tgctcacccc aatctttcct cctgacaggt tgatctttat ccctctacta      480

aattgcccca agtgtatcaa gtagactaga tctcgcgaaa gaacagccta ataaactccg      540

aagcatgatg gcctctatcc ggaaaacgtt aagagatgtg gcaacaggag ggcacataga      600

atttttaaag acgctgaaga atgctatcat agtccgtaaa aatgtgatag tactttgttt      660

agtgcgtacg ccacttattc ggggccaata gctaaaccca ggtttgctgg cagcaaattc      720

aactgtagat tgaatctctc taacaataat ggtgttcaat cccctggctg gtcacgggga      780

ggactatctt gcgtgatccg cttggaaaat gttgtgtatc cctttctcaa ttgcggaaag      840

catctgctac ttcccatagg caccagttac ccaattgata tttccaaaaa agattaccat      900

atgttcatct agaagtataa atacaagtgg acattcaatg aatatttcat tcaattagtc      960

attgacactt tcatcaactt actacgtctt attcaacaat                           1000


<210>  49
<211>  1000
<212>  DNA
<213>  Pichia pastoris

<400>  49
tatacggtct atccactttg gaaacgatgt agttgaaacg gggaagtaat agtggttccc       60

aaacgacatg aagaggttat ataagtttgc aagagggtga caccatttta gttgtggttc      120

ccgggtattt ttttaatctt tttagtctaa gatagcctcc ccagatatta ccgagttggg      180

ccatttgggg cggtatcggt ggtatctgat ggtagcgcgt ttttacatgc ctgtgcattg      240

aactggcaaa gagtatacta tcgtggggcc ctgaaggagg cagcaaatgg accgtcaatt      300

ggttgatcag ggactcaaga caggtattga gcttttcaaa caaaaagagt ataggcgctg      360

ctacaaggca tttacttcta ctatcaattt cattgagaat gatcccgagt tggccgccag      420

ctgtgtatct caactgatat ctctgttaga ttgtagggca gcctgtttgg aaaagctaga      480

tcaattgaat atggccttga aagatggtct taaaatgatc aagagagagt gccacaactg      540

caagggttat ttgagaactt gcaaaatttt agacctacaa gggaagatca gtgaggcttt      600

gtctacagca agagaaggga tctccataat agaaactaga agagatcagg ataatcaatt      660

tagatattcc aaggttcttt tggaacaatt aaaggaactg aaaaatgcac tgaaaatcaa      720

attggacaag aaaaatcagc tacacttcaa agttttaaag tttgacgcac cagtgccttg      780

tacaaagaaa ctaagattag tcactccaag aacaatagat ccttccattt ttttgccgat      840

agagctagtg aagctgatct ttcgcctgtt gaatttctca gacatgtatg cctgtttatt      900

ggtctcaaca aaatggaact caattatatc ctcatcaccg gaactgtttc gaaaacttca      960

gttgaaatcc caactgtcca acaaggcgtt aaacaattgt                           1000


