                         SEQUENCE LISTING

<110>  INTELLIA THERAPEUTICS, INC.
 
<120>  COMPOSITIONS AND METHODS COMPRISING A TTR GUIDE RNA AND A 
       POLYNUCLEOTIDE ENCODING AN RNA-GUIDED DNA BINDING AGENT

<130>  01155-0028-00PCT

<150>  US 62/825,637
<151>  2019-03-28

<160>  396   

<170>  PatentIn version 3.5

<210>  1
<211>  4411
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript with 5 UTR of HSD, ORF corresponding 
       to SEQ ID NO: 204, Kozak sequence, and 3 UTR of ALB

<400>  1
gggtcccgca gtcggcgtcc agcggctctg cttgttcgtg tgtgtgtcgt tgcaggcctt       60

attcggatcc gccaccatgg acaagaagta cagcatcgga ctggacatcg gaacaaacag      120

cgtcggatgg gcagtcatca cagacgaata caaggtcccg agcaagaagt tcaaggtcct      180

gggaaacaca gacagacaca gcatcaagaa gaacctgatc ggagcactgc tgttcgacag      240

cggagaaaca gcagaagcaa caagactgaa gagaacagca agaagaagat acacaagaag      300

aaagaacaga atctgctacc tgcaggaaat cttcagcaac gaaatggcaa aggtcgacga      360

cagcttcttc cacagactgg aagaaagctt cctggtcgaa gaagacaaga agcacgaaag      420

acacccgatc ttcggaaaca tcgtcgacga agtcgcatac cacgaaaagt acccgacaat      480

ctaccacctg agaaagaagc tggtcgacag cacagacaag gcagacctga gactgatcta      540

cctggcactg gcacacatga tcaagttcag aggacacttc ctgatcgaag gagacctgaa      600

cccggacaac agcgacgtcg acaagctgtt catccagctg gtccagacat acaaccagct      660

gttcgaagaa aacccgatca acgcaagcgg agtcgacgca aaggcaatcc tgagcgcaag      720

actgagcaag agcagaagac tggaaaacct gatcgcacag ctgccgggag aaaagaagaa      780

cggactgttc ggaaacctga tcgcactgag cctgggactg acaccgaact tcaagagcaa      840

cttcgacctg gcagaagacg caaagctgca gctgagcaag gacacatacg acgacgacct      900

ggacaacctg ctggcacaga tcggagacca gtacgcagac ctgttcctgg cagcaaagaa      960

cctgagcgac gcaatcctgc tgagcgacat cctgagagtc aacacagaaa tcacaaaggc     1020

accgctgagc gcaagcatga tcaagagata cgacgaacac caccaggacc tgacactgct     1080

gaaggcactg gtcagacagc agctgccgga aaagtacaag gaaatcttct tcgaccagag     1140

caagaacgga tacgcaggat acatcgacgg aggagcaagc caggaagaat tctacaagtt     1200

catcaagccg atcctggaaa agatggacgg aacagaagaa ctgctggtca agctgaacag     1260

agaagacctg ctgagaaagc agagaacatt cgacaacgga agcatcccgc accagatcca     1320

cctgggagaa ctgcacgcaa tcctgagaag acaggaagac ttctacccgt tcctgaagga     1380

caacagagaa aagatcgaaa agatcctgac attcagaatc ccgtactacg tcggaccgct     1440

ggcaagagga aacagcagat tcgcatggat gacaagaaag agcgaagaaa caatcacacc     1500

gtggaacttc gaagaagtcg tcgacaaggg agcaagcgca cagagcttca tcgaaagaat     1560

gacaaacttc gacaagaacc tgccgaacga aaaggtcctg ccgaagcaca gcctgctgta     1620

cgaatacttc acagtctaca acgaactgac aaaggtcaag tacgtcacag aaggaatgag     1680

aaagccggca ttcctgagcg gagaacagaa gaaggcaatc gtcgacctgc tgttcaagac     1740

aaacagaaag gtcacagtca agcagctgaa ggaagactac ttcaagaaga tcgaatgctt     1800

cgacagcgtc gaaatcagcg gagtcgaaga cagattcaac gcaagcctgg gaacatacca     1860

cgacctgctg aagatcatca aggacaagga cttcctggac aacgaagaaa acgaagacat     1920

cctggaagac atcgtcctga cactgacact gttcgaagac agagaaatga tcgaagaaag     1980

actgaagaca tacgcacacc tgttcgacga caaggtcatg aagcagctga agagaagaag     2040

atacacagga tggggaagac tgagcagaaa gctgatcaac ggaatcagag acaagcagag     2100

cggaaagaca atcctggact tcctgaagag cgacggattc gcaaacagaa acttcatgca     2160

gctgatccac gacgacagcc tgacattcaa ggaagacatc cagaaggcac aggtcagcgg     2220

acagggagac agcctgcacg aacacatcgc aaacctggca ggaagcccgg caatcaagaa     2280

gggaatcctg cagacagtca aggtcgtcga cgaactggtc aaggtcatgg gaagacacaa     2340

gccggaaaac atcgtcatcg aaatggcaag agaaaaccag acaacacaga agggacagaa     2400

gaacagcaga gaaagaatga agagaatcga agaaggaatc aaggaactgg gaagccagat     2460

cctgaaggaa cacccggtcg aaaacacaca gctgcagaac gaaaagctgt acctgtacta     2520

cctgcagaac ggaagagaca tgtacgtcga ccaggaactg gacatcaaca gactgagcga     2580

ctacgacgtc gaccacatcg tcccgcagag cttcctgaag gacgacagca tcgacaacaa     2640

ggtcctgaca agaagcgaca agaacagagg aaagagcgac aacgtcccga gcgaagaagt     2700

cgtcaagaag atgaagaact actggagaca gctgctgaac gcaaagctga tcacacagag     2760

aaagttcgac aacctgacaa aggcagagag aggaggactg agcgaactgg acaaggcagg     2820

attcatcaag agacagctgg tcgaaacaag acagatcaca aagcacgtcg cacagatcct     2880

ggacagcaga atgaacacaa agtacgacga aaacgacaag ctgatcagag aagtcaaggt     2940

catcacactg aagagcaagc tggtcagcga cttcagaaag gacttccagt tctacaaggt     3000

cagagaaatc aacaactacc accacgcaca cgacgcatac ctgaacgcag tcgtcggaac     3060

agcactgatc aagaagtacc cgaagctgga aagcgaattc gtctacggag actacaaggt     3120

ctacgacgtc agaaagatga tcgcaaagag cgaacaggaa atcggaaagg caacagcaaa     3180

gtacttcttc tacagcaaca tcatgaactt cttcaagaca gaaatcacac tggcaaacgg     3240

agaaatcaga aagagaccgc tgatcgaaac aaacggagaa acaggagaaa tcgtctggga     3300

caagggaaga gacttcgcaa cagtcagaaa ggtcctgagc atgccgcagg tcaacatcgt     3360

caagaagaca gaagtccaga caggaggatt cagcaaggaa agcatcctgc cgaagagaaa     3420

cagcgacaag ctgatcgcaa gaaagaagga ctgggacccg aagaagtacg gaggattcga     3480

cagcccgaca gtcgcataca gcgtcctggt cgtcgcaaag gtcgaaaagg gaaagagcaa     3540

gaagctgaag agcgtcaagg aactgctggg aatcacaatc atggaaagaa gcagcttcga     3600

aaagaacccg atcgacttcc tggaagcaaa gggatacaag gaagtcaaga aggacctgat     3660

catcaagctg ccgaagtaca gcctgttcga actggaaaac ggaagaaaga gaatgctggc     3720

aagcgcagga gaactgcaga agggaaacga actggcactg ccgagcaagt acgtcaactt     3780

cctgtacctg gcaagccact acgaaaagct gaagggaagc ccggaagaca acgaacagaa     3840

gcagctgttc gtcgaacagc acaagcacta cctggacgaa atcatcgaac agatcagcga     3900

attcagcaag agagtcatcc tggcagacgc aaacctggac aaggtcctga gcgcatacaa     3960

caagcacaga gacaagccga tcagagaaca ggcagaaaac atcatccacc tgttcacact     4020

gacaaacctg ggagcaccgg cagcattcaa gtacttcgac acaacaatcg acagaaagag     4080

atacacaagc acaaaggaag tcctggacgc aacactgatc caccagagca tcacaggact     4140

gtacgaaaca agaatcgacc tgagccagct gggaggagac ggaggaggaa gcccgaagaa     4200

gaagagaaag gtctagctag ccatcacatt taaaagcatc tcagcctacc atgagaataa     4260

gagaaagaaa atgaagatca atagcttatt catctctttt tctttttcgt tggtgtaaag     4320

ccaacaccct gtctaaaaaa cataaatttc tttaatcatt ttgcctcttt tctctgtgct     4380

tcaattaata aaaaatggaa agaacctcga g                                    4411


<210>  2
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000739 - sgRNA negative control


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  2
gaucacgucg gccguuggcg guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  3
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: modified sgRNA sequence (N may be any natural or 
       non-natural nucleotide)


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  3
nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  4
<211>  105
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 30/30/39 poly-A sequence

<400>  4
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gcgaaaaaaa aaaaaaaaaa aaaaaaaaaa       60

aaaccgaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa                      105


<210>  5
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003335 gRNA targeting Human TTR (Exon 1)

<400>  5
cugcuccucc ucugccuugc                                                   20


<210>  6
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003336 gRNA targeting Human TTR (Exon 1)

<400>  6
ccuccucugc cuugcuggac                                                   20


<210>  7
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003337 gRNA targeting Human TTR (Exon 1)

<400>  7
ccaguccagc aaggcagagg                                                   20


<210>  8
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003338 gRNA targeting Human TTR (Exon 1)

<400>  8
auaccagucc agcaaggcag                                                   20


<210>  9
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003339 gRNA targeting Human TTR (Exon 1)

<400>  9
acacaaauac caguccagca                                                   20


<210>  10
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003340 gRNA targeting Human TTR (Exon 1)

<400>  10
uggacuggua uuugugucug                                                   20


<210>  11
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003341 gRNA targeting Human TTR (Exon 1)

<400>  11
cugguauuug ugucugaggc                                                   20


<210>  12
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003342 gRNA targeting Human TTR (Exon 2)

<400>  12
cuucucuaca cccagggcac                                                   20


<210>  13
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003343 gRNA targeting Human TTR (Exon 2)

<400>  13
cagaggacac uuggauucac                                                   20


<210>  14
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003344 gRNA targeting Human TTR (Exon 2)

<400>  14
uuugaccauc agaggacacu                                                   20


<210>  15
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003345 gRNA targeting Human TTR (Exon 2)

<400>  15
ucuagaacuu ugaccaucag                                                   20


<210>  16
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003346 gRNA targeting Human TTR (Exon 2)

<400>  16
aaaguucuag augcuguccg                                                   20


<210>  17
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003347 gRNA targeting Human TTR (Exon 2)

<400>  17
cauugauggc aggacugccu                                                   20


<210>  18
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003348 gRNA targeting Human TTR (Exon 2)

<400>  18
aggcaguccu gccaucaaug                                                   20


<210>  19
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003349 gRNA targeting Human TTR (Exon 2)

<400>  19
ugcacggcca cauugauggc                                                   20


<210>  20
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003350 gRNA targeting Human TTR (Exon 2)

<400>  20
cacaugcacg gccacauuga                                                   20


<210>  21
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003351 gRNA targeting Human TTR (Exon 2)

<400>  21
agccuuucug aacacaugca                                                   20


<210>  22
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003352 gRNA targeting Human TTR (Exon 2)

<400>  22
gaaaggcugc ugaugacacc                                                   20


<210>  23
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003353 gRNA targeting Human TTR (Exon 2)

<400>  23
aaaggcugcu gaugacaccu                                                   20


<210>  24
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003354 gRNA targeting Human TTR (Exon 2)

<400>  24
accugggagc cauuugccuc                                                   20


<210>  25
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003355 gRNA targeting Human TTR (Exon 2)

<400>  25
cccagaggca aauggcuccc                                                   20


<210>  26
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003356 gRNA targeting Human TTR (Exon 2)

<400>  26
gcaacuuacc cagaggcaaa                                                   20


<210>  27
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003357 gRNA targeting Human TTR (Exon 2)

<400>  27
uucuuuggca acuuacccag                                                   20


<210>  28
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003358 gRNA targeting Human TTR (Exon 3)

<400>  28
augcagcucu ccagacucac                                                   20


<210>  29
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003359 gRNA targeting Human TTR (Exon 3)

<400>  29
agugagucug gagagcugca                                                   20


<210>  30
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003360 gRNA targeting Human TTR (Exon 3)

<400>  30
gugagucugg agagcugcau                                                   20


<210>  31
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003361 gRNA targeting Human TTR (Exon 3)

<400>  31
gcugcauggg cucacaacug                                                   20


<210>  32
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003362 gRNA targeting Human TTR (Exon 3)

<400>  32
gcaugggcuc acaacugagg                                                   20


<210>  33
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003363 gRNA targeting Human TTR (Exon 3)

<400>  33
acugaggagg aauuuguaga                                                   20


<210>  34
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003364 gRNA targeting Human TTR (Exon 3)

<400>  34
cugaggagga auuuguagaa                                                   20


<210>  35
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003365 gRNA targeting Human TTR (Exon 3)

<400>  35
uguagaaggg auauacaaag                                                   20


<210>  36
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003366 gRNA targeting Human TTR (Exon 3)

<400>  36
aaauagacac caaaucuuac                                                   20


<210>  37
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003367 gRNA targeting Human TTR (Exon 3)

<400>  37
agacaccaaa ucuuacugga                                                   20


<210>  38
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003368 gRNA targeting Human TTR (Exon 3)

<400>  38
aagugccuuc caguaagauu                                                   20


<210>  39
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003369 gRNA targeting Human TTR (Exon 3)

<400>  39
cucugcaugc ucauggaaug                                                   20


<210>  40
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003370 gRNA targeting Human TTR (Exon 3)

<400>  40
ccucugcaug cucauggaau                                                   20


<210>  41
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003371 gRNA targeting Human TTR (Exon 3)

<400>  41
accucugcau gcucauggaa                                                   20


<210>  42
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003372 gRNA targeting Human TTR (Exon 3)

<400>  42
uacucaccuc ugcaugcuca                                                   20


<210>  43
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003373 gRNA targeting Human TTR (Exon 4)

<400>  43
guauucacag ccaacgacuc                                                   20


<210>  44
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003374 gRNA targeting Human TTR (Exon 4)

<400>  44
gcggcggggg ccggagucgu                                                   20


<210>  45
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003375 gRNA targeting Human TTR (Exon 4)

<400>  45
aaugguguag cggcgggggc                                                   20


<210>  46
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003376 gRNA targeting Human TTR (Exon 4)

<400>  46
cggcaauggu guagcggcgg                                                   20


<210>  47
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003377 gRNA targeting Human TTR (Exon 4)

<400>  47
gcggcaaugg uguagcggcg                                                   20


<210>  48
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003378 gRNA targeting Human TTR (Exon 4)

<400>  48
ggcggcaaug guguagcggc                                                   20


<210>  49
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003379 gRNA targeting Human TTR (Exon 4)

<400>  49
gggcggcaau gguguagcgg                                                   20


<210>  50
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003380 gRNA targeting Human TTR (Exon 4)

<400>  50
gcagggcggc aaugguguag                                                   20


<210>  51
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003381 gRNA targeting Human TTR (Exon 4)

<400>  51
ggggcucagc agggcggcaa                                                   20


<210>  52
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003382 gRNA targeting Human TTR (Exon 4)

<400>  52
ggaguagggg cucagcaggg                                                   20


<210>  53
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003383 gRNA targeting Human TTR (Exon 4)

<400>  53
auaggaguag gggcucagca                                                   20


<210>  54
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003384 gRNA targeting Human TTR (Exon 4)

<400>  54
aauaggagua ggggcucagc                                                   20


<210>  55
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003385 gRNA targeting Human TTR (Exon 4)

<400>  55
ccccuacucc uauuccacca                                                   20


<210>  56
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003386 gRNA targeting Human TTR (Exon 4)

<400>  56
ccguggugga auaggaguag                                                   20


<210>  57
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003387 gRNA targeting Human TTR (Exon 4)

<400>  57
gccguggugg aauaggagua                                                   20


<210>  58
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003388 gRNA targeting Human TTR (Exon 4)

<400>  58
gacgacagcc gugguggaau                                                   20


<210>  59
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003389 gRNA targeting Human TTR (Exon 4)

<400>  59
auuggugacg acagccgugg                                                   20


<210>  60
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003390 gRNA targeting Human TTR (Exon 4)

<400>  60
gggauuggug acgacagccg                                                   20


<210>  61
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003391 gRNA targeting Human TTR (Exon 4)

<400>  61
ggcugucguc accaauccca                                                   20


<210>  62
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR003392 gRNA targeting Human TTR (Exon 4)

<400>  62
agucccucau uccuugggau                                                   20


<210>  63
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005298 gRNA targeting Human TTR (Exon 1)

<400>  63
uccacucauu cuuggcagga                                                   20


<210>  64
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005299 gRNA targeting Human TTR (Exon 4)

<400>  64
agccguggug gaauaggagu                                                   20


<210>  65
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005300 gRNA targeting Human TTR (Exon 1)

<400>  65
ucacagaaac acucaccgua                                                   20


<210>  66
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005301 gRNA targeting Human TTR (Exon 1)

<400>  66
gucacagaaa cacucaccgu                                                   20


<210>  67
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005302 gRNA targeting Human TTR (Exon 2)

<400>  67
acgugucuuc ucuacaccca                                                   20


<210>  68
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005303 gRNA targeting Human TTR (Exon 2)

<400>  68
ugaauccaag uguccucuga                                                   20


<210>  69
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005304 gRNA targeting Human TTR (Exon 2)

<400>  69
ggccgugcau guguucagaa                                                   20


<210>  70
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005305 gRNA targeting Human TTR (Exon 3)

<400>  70
uauaggaaaa ccagugaguc                                                   20


<210>  71
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005306 gRNA targeting Human TTR (Exon 3)

<400>  71
aaaucuuacu ggaaggcacu                                                   20


<210>  72
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005307 gRNA targeting Human TTR (Exon 4)

<400>  72
ugucugucuu cucucauagg                                                   20


<210>  73
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR000689 gRNA targeting Cyno TTR

<400>  73
acacaaauac caguccagcg                                                   20


<210>  74
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005364 gRNA targeting Cyno TTR

<400>  74
aaaggcugcu gaugagaccu                                                   20


<210>  75
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005365 gRNA targeting Cyno TTR

<400>  75
cauugacagc aggacugccu                                                   20


<210>  76
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005366 gRNA targeting Cyno TTR

<400>  76
auaccagucc agcgaggcag                                                   20


<210>  77
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005367 gRNA targeting Cyno TTR

<400>  77
ccaguccagc gaggcagagg                                                   20


<210>  78
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005368 gRNA targeting Cyno TTR

<400>  78
ccuccucugc cucgcuggac                                                   20


<210>  79
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005369 gRNA targeting Cyno TTR

<400>  79
aaaguucuag augccguccg                                                   20


<210>  80
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005370 gRNA targeting Cyno TTR

<400>  80
acuugucuuc ucuauaccca                                                   20


<210>  81
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005371 gRNA targeting Cyno TTR

<400>  81
aagugacuuc caguaagauu                                                   20


<210>  82
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CR005372 gRNA targeting Cyno TTR

<400>  82
aaaaggcugc ugaugagacc                                                   20


<210>  83

<400>  83
000

<210>  84

<400>  84
000

<210>  85

<400>  85
000

<210>  86

<400>  86
000

<210>  87
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000480 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  87
aaaggcugcu gaugacaccu guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  88
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000481 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  88
ucuagaacuu ugaccaucag guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  89
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000482 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  89
uguagaaggg auauacaaag guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  90
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000483 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  90
uccacucauu cuuggcagga guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  91
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000484 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  91
agacaccaaa ucuuacugga guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  92
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000485 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  92
ccuccucugc cuugcuggac guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  93
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000486 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  93
acacaaauac caguccagca guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  94
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000487 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  94
uucuuuggca acuuacccag guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  95
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000488 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  95
aaaguucuag augcuguccg guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  96
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000489 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  96
uuugaccauc agaggacacu guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  97
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000490 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  97
aaauagacac caaaucuuac guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  98
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000491 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  98
auaccagucc agcaaggcag guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  99
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000492 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  99
cuucucuaca cccagggcac guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  100
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000493 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  100
aagugccuuc caguaagauu guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  101
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000494 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  101
gugagucugg agagcugcau guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  102
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000495 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  102
cagaggacac uuggauucac guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  103
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000496 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  103
ggccgugcau guguucagaa guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  104
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000497 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  104
cugcuccucc ucugccuugc guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  105
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000498 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  105
agugagucug gagagcugca guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  106
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000499 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  106
ugaauccaag uguccucuga guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  107
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000500 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  107
ccaguccagc aaggcagagg guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  108
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000501 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  108
ucacagaaac acucaccgua guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  109
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000567 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  109
gaaaggcugc ugaugacacc guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  110
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000568 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  110
ggcugucguc accaauccca guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  111
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000570 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  111
cauugauggc aggacugccu guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  112
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000571 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  112
gucacagaaa cacucaccgu guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  113
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000572 sgRNA modified sequence targeting Human TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  113
ccccuacucc uauuccacca guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  114
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000502 sgRNA modified sequence targeting Cyno TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  114
acacaaauac caguccagcg guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  115
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000503 sgRNA modified sequence targeting Cyno TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  115
aaaaggcugc ugaugagacc guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  116
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000504 sgRNA modified sequence targeting Cyno TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  116
aaaggcugcu gaugagaccu guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  117
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000505 sgRNA modified sequence targeting Cyno TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  117
cauugacagc aggacugccu guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  118
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000506 sgRNA modified sequence targeting Cyno TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  118
auaccagucc agcgaggcag guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  119
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000507 sgRNA modified sequence targeting Cyno TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  119
ccaguccagc gaggcagagg guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  120
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000508 sgRNA modified sequence targeting Cyno TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  120
ccuccucugc cucgcuggac guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  121
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000509 sgRNA modified sequence targeting Cyno TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  121
aaaguucuag augccguccg guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  122
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000510 sgRNA modified sequence targeting Cyno TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  122
acuugucuuc ucuauaccca guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  123
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000511 sgRNA modified sequence targeting Cyno TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  123
aagugacuuc caguaagauu guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  124
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G000282 sgRNA modified sequence targeting Mouse TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  124
uuacagccac gucuacagca guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  125
<211>  80
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: exemplary nucleotide sequence

<400>  125
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugcuuuu                                                   80


<210>  126
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: exemplary nucleotide sequence

<400>  126
guuuuagagc uaugcuguuu ug                                                22


<210>  127

<400>  127
000

<210>  128

<400>  128
000

<210>  129

<400>  129
000

<210>  130

<400>  130
000

<210>  131

<400>  131
000

<210>  132

<400>  132
000

<210>  133

<400>  133
000

<210>  134

<400>  134
000

<210>  135

<400>  135
000

<210>  136

<400>  136
000

<210>  137

<400>  137
000

<210>  138

<400>  138
000

<210>  139

<400>  139
000

<210>  140

<400>  140
000

<210>  141

<400>  141
000

<210>  142

<400>  142
000

<210>  143

<400>  143
000

<210>  144

<400>  144
000

<210>  145

<400>  145
000

<210>  146

<400>  146
000

<210>  147

<400>  147
000

<210>  148

<400>  148
000

<210>  149

<400>  149
000

<210>  150

<400>  150
000

<210>  151

<400>  151
000

<210>  152

<400>  152
000

<210>  153

<400>  153
000

<210>  154

<400>  154
000

<210>  155

<400>  155
000

<210>  156

<400>  156
000

<210>  157

<400>  157
000

<210>  158

<400>  158
000

<210>  159

<400>  159
000

<210>  160

<400>  160
000

<210>  161

<400>  161
000

<210>  162

<400>  162
000

<210>  163

<400>  163
000

<210>  164

<400>  164
000

<210>  165

<400>  165
000

<210>  166

<400>  166
000

<210>  167

<400>  167
000

<210>  168

<400>  168
000

<210>  169

<400>  169
000

<210>  170

<400>  170
000

<210>  171

<400>  171
000

<210>  172

<400>  172
000

<210>  173

<400>  173
000

<210>  174

<400>  174
000

<210>  175

<400>  175
000

<210>  176

<400>  176
000

<210>  177

<400>  177
000

<210>  178

<400>  178
000

<210>  179

<400>  179
000

<210>  180

<400>  180
000

<210>  181

<400>  181
000

<210>  182

<400>  182
000

<210>  183

<400>  183
000

<210>  184

<400>  184
000

<210>  185

<400>  185
000

<210>  186

<400>  186
000

<210>  187

<400>  187
000

<210>  188

<400>  188
000

<210>  189

<400>  189
000

<210>  190

<400>  190
000

<210>  191

<400>  191
000

<210>  192

<400>  192
000

<210>  193

<400>  193
000

<210>  194

<400>  194
000

<210>  195

<400>  195
000

<210>  196

<400>  196
000

<210>  197

<400>  197
000

<210>  198

<400>  198
000

<210>  199

<400>  199
000

<210>  200

<400>  200
000

<210>  201

<400>  201
000

<210>  202

<400>  202
000

<210>  203
<211>  1379
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 amino acid sequence

<400>  203

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Gly Gly  Gly Ser Pro Lys Lys  Lys Arg Lys Val 
    1370                 1375                 


<210>  204
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 mRNA open reading frame (ORF) 2

<400>  204
auggacaaga aguacagcau cggacuggac aucggaacaa acagcgucgg augggcaguc       60

aucacagacg aauacaaggu cccgagcaag aaguucaagg uccugggaaa cacagacaga      120

cacagcauca agaagaaccu gaucggagca cugcuguucg acagcggaga aacagcagaa      180

gcaacaagac ugaagagaac agcaagaaga agauacacaa gaagaaagaa cagaaucugc      240

uaccugcagg aaaucuucag caacgaaaug gcaaaggucg acgacagcuu cuuccacaga      300

cuggaagaaa gcuuccuggu cgaagaagac aagaagcacg aaagacaccc gaucuucgga      360

aacaucgucg acgaagucgc auaccacgaa aaguacccga caaucuacca ccugagaaag      420

aagcuggucg acagcacaga caaggcagac cugagacuga ucuaccuggc acuggcacac      480

augaucaagu ucagaggaca cuuccugauc gaaggagacc ugaacccgga caacagcgac      540

gucgacaagc uguucaucca gcugguccag acauacaacc agcuguucga agaaaacccg      600

aucaacgcaa gcggagucga cgcaaaggca auccugagcg caagacugag caagagcaga      660

agacuggaaa accugaucgc acagcugccg ggagaaaaga agaacggacu guucggaaac      720

cugaucgcac ugagccuggg acugacaccg aacuucaaga gcaacuucga ccuggcagaa      780

gacgcaaagc ugcagcugag caaggacaca uacgacgacg accuggacaa ccugcuggca      840

cagaucggag accaguacgc agaccuguuc cuggcagcaa agaaccugag cgacgcaauc      900

cugcugagcg acauccugag agucaacaca gaaaucacaa aggcaccgcu gagcgcaagc      960

augaucaaga gauacgacga acaccaccag gaccugacac ugcugaaggc acuggucaga     1020

cagcagcugc cggaaaagua caaggaaauc uucuucgacc agagcaagaa cggauacgca     1080

ggauacaucg acggaggagc aagccaggaa gaauucuaca aguucaucaa gccgauccug     1140

gaaaagaugg acggaacaga agaacugcug gucaagcuga acagagaaga ccugcugaga     1200

aagcagagaa cauucgacaa cggaagcauc ccgcaccaga uccaccuggg agaacugcac     1260

gcaauccuga gaagacagga agacuucuac ccguuccuga aggacaacag agaaaagauc     1320

gaaaagaucc ugacauucag aaucccguac uacgucggac cgcuggcaag aggaaacagc     1380

agauucgcau ggaugacaag aaagagcgaa gaaacaauca caccguggaa cuucgaagaa     1440

gucgucgaca agggagcaag cgcacagagc uucaucgaaa gaaugacaaa cuucgacaag     1500

aaccugccga acgaaaaggu ccugccgaag cacagccugc uguacgaaua cuucacaguc     1560

uacaacgaac ugacaaaggu caaguacguc acagaaggaa ugagaaagcc ggcauuccug     1620

agcggagaac agaagaaggc aaucgucgac cugcuguuca agacaaacag aaaggucaca     1680

gucaagcagc ugaaggaaga cuacuucaag aagaucgaau gcuucgacag cgucgaaauc     1740

agcggagucg aagacagauu caacgcaagc cugggaacau accacgaccu gcugaagauc     1800

aucaaggaca aggacuuccu ggacaacgaa gaaaacgaag acauccugga agacaucguc     1860

cugacacuga cacuguucga agacagagaa augaucgaag aaagacugaa gacauacgca     1920

caccuguucg acgacaaggu caugaagcag cugaagagaa gaagauacac aggaugggga     1980

agacugagca gaaagcugau caacggaauc agagacaagc agagcggaaa gacaauccug     2040

gacuuccuga agagcgacgg auucgcaaac agaaacuuca ugcagcugau ccacgacgac     2100

agccugacau ucaaggaaga cauccagaag gcacagguca gcggacaggg agacagccug     2160

cacgaacaca ucgcaaaccu ggcaggaagc ccggcaauca agaagggaau ccugcagaca     2220

gucaaggucg ucgacgaacu ggucaagguc augggaagac acaagccgga aaacaucguc     2280

aucgaaaugg caagagaaaa ccagacaaca cagaagggac agaagaacag cagagaaaga     2340

augaagagaa ucgaagaagg aaucaaggaa cugggaagcc agauccugaa ggaacacccg     2400

gucgaaaaca cacagcugca gaacgaaaag cuguaccugu acuaccugca gaacggaaga     2460

gacauguacg ucgaccagga acuggacauc aacagacuga gcgacuacga cgucgaccac     2520

aucgucccgc agagcuuccu gaaggacgac agcaucgaca acaagguccu gacaagaagc     2580

gacaagaaca gaggaaagag cgacaacguc ccgagcgaag aagucgucaa gaagaugaag     2640

aacuacugga gacagcugcu gaacgcaaag cugaucacac agagaaaguu cgacaaccug     2700

acaaaggcag agagaggagg acugagcgaa cuggacaagg caggauucau caagagacag     2760

cuggucgaaa caagacagau cacaaagcac gucgcacaga uccuggacag cagaaugaac     2820

acaaaguacg acgaaaacga caagcugauc agagaaguca aggucaucac acugaagagc     2880

aagcugguca gcgacuucag aaaggacuuc caguucuaca aggucagaga aaucaacaac     2940

uaccaccacg cacacgacgc auaccugaac gcagucgucg gaacagcacu gaucaagaag     3000

uacccgaagc uggaaagcga auucgucuac ggagacuaca aggucuacga cgucagaaag     3060

augaucgcaa agagcgaaca ggaaaucgga aaggcaacag caaaguacuu cuucuacagc     3120

aacaucauga acuucuucaa gacagaaauc acacuggcaa acggagaaau cagaaagaga     3180

ccgcugaucg aaacaaacgg agaaacagga gaaaucgucu gggacaaggg aagagacuuc     3240

gcaacaguca gaaagguccu gagcaugccg caggucaaca ucgucaagaa gacagaaguc     3300

cagacaggag gauucagcaa ggaaagcauc cugccgaaga gaaacagcga caagcugauc     3360

gcaagaaaga aggacuggga cccgaagaag uacggaggau ucgacagccc gacagucgca     3420

uacagcgucc uggucgucgc aaaggucgaa aagggaaaga gcaagaagcu gaagagcguc     3480

aaggaacugc ugggaaucac aaucauggaa agaagcagcu ucgaaaagaa cccgaucgac     3540

uuccuggaag caaagggaua caaggaaguc aagaaggacc ugaucaucaa gcugccgaag     3600

uacagccugu ucgaacugga aaacggaaga aagagaaugc uggcaagcgc aggagaacug     3660

cagaagggaa acgaacuggc acugccgagc aaguacguca acuuccugua ccuggcaagc     3720

cacuacgaaa agcugaaggg aagcccggaa gacaacgaac agaagcagcu guucgucgaa     3780

cagcacaagc acuaccugga cgaaaucauc gaacagauca gcgaauucag caagagaguc     3840

auccuggcag acgcaaaccu ggacaagguc cugagcgcau acaacaagca cagagacaag     3900

ccgaucagag aacaggcaga aaacaucauc caccuguuca cacugacaaa ccugggagca     3960

ccggcagcau ucaaguacuu cgacacaaca aucgacagaa agagauacac aagcacaaag     4020

gaaguccugg acgcaacacu gauccaccag agcaucacag gacuguacga aacaagaauc     4080

gaccugagcc agcugggagg agacggagga ggaagcccga agaagaagag aaaggucuag     4140


<210>  205
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 mRNA ORF 1

<400>  205
auggauaaga aguacucaau cgggcuggau aucggaacua auuccguggg uugggcagug       60

aucacggaug aauacaaagu gccguccaag aaguucaagg uccuggggaa caccgauaga      120

cacagcauca agaaaaaucu caucggagcc cugcuguuug acuccggcga aaccgcagaa      180

gcgacccggc ucaaacguac cgcgaggcga cgcuacaccc ggcggaagaa ucgcaucugc      240

uaucugcaag agaucuuuuc gaacgaaaug gcaaaggucg acgacagcuu cuuccaccgc      300

cuggaagaau cuuuccuggu ggaggaggac aagaagcaug aacggcaucc uaucuuugga      360

aacaucgucg acgaaguggc guaccacgaa aaguacccga ccaucuacca ucugcggaag      420

aaguugguug acucaacuga caaggccgac cucagauuga ucuacuuggc ccucgcccau      480

augaucaaau uccgcggaca cuuccugauc gaaggcgauc ugaacccuga uaacuccgac      540

guggauaagc uuuucauuca acuggugcag accuacaacc aacuguucga agaaaaccca      600

aucaaugcua gcggcgucga ugccaaggcc auccuguccg cccggcuguc gaagucgcgg      660

cgccucgaaa accugaucgc acagcugccg ggagagaaaa agaacggacu uuucggcaac      720

uugaucgcuc ucucacuggg acucacuccc aauuucaagu ccaauuuuga ccuggccgag      780

gacgcgaagc ugcaacucuc aaaggacacc uacgacgacg acuuggacaa uuugcuggca      840

caaauuggcg aucaguacgc ggaucuguuc cuugccgcua agaaccuuuc ggacgcaauc      900

uugcuguccg auauccugcg cgugaacacc gaaauaacca aagcgccgcu uagcgccucg      960

augauuaagc gguacgacga gcaucaccag gaucucacgc ugcucaaagc gcucgugaga     1020

cagcaacugc cugaaaagua caaggagauc uucuucgacc aguccaagaa uggguacgca     1080

ggguacaucg auggaggcgc uagccaggaa gaguucuaua aguucaucaa gccaauccug     1140

gaaaagaugg acggaaccga agaacugcug gucaagcuga acagggagga ucugcuccgg     1200

aaacagagaa ccuuugacaa cggauccauu ccccaccaga uccaucuggg ugagcugcac     1260

gccaucuugc ggcgccagga ggacuuuuac ccauuccuca aggacaaccg ggaaaagauc     1320

gagaaaauuc ugacguuccg caucccguau uacgugggcc cacuggcgcg cggcaauucg     1380

cgcuucgcgu ggaugacuag aaaaucagag gaaaccauca cuccuuggaa uuucgaggaa     1440

guuguggaua agggagcuuc ggcacaaagc uucaucgaac gaaugaccaa cuucgacaag     1500

aaucucccaa acgagaaggu gcuuccuaag cacagccucc uuuacgaaua cuucacuguc     1560

uacaacgaac ugacuaaagu gaaauacguu acugaaggaa ugaggaagcc ggccuuucug     1620

uccggagaac agaagaaagc aauugucgau cugcuguuca agaccaaccg caaggugacc     1680

gucaagcagc uuaaagagga cuacuucaag aagaucgagu guuucgacuc aguggaaauc     1740

agcggggugg aggacagauu caacgcuucg cugggaaccu aucaugaucu ccugaagauc     1800

aucaaggaca aggacuuccu ugacaacgag gagaacgagg acauccugga agauaucguc     1860

cugaccuuga cccuuuucga ggaucgcgag augaucgagg agaggcuuaa gaccuacgcu     1920

caucucuucg acgauaaggu caugaaacaa cucaagcgcc gccgguacac ugguuggggc     1980

cgccucuccc gcaagcugau caacgguauu cgcgauaaac agagcgguaa aacuauccug     2040

gauuuccuca aaucggaugg cuucgcuaau cguaacuuca ugcaauugau ccacgacgac     2100

agccugaccu uuaaggagga cauccaaaaa gcacaagugu ccggacaggg agacucacuc     2160

caugaacaca ucgcgaaucu ggccgguucg ccggcgauua agaagggaau ucugcaaacu     2220

gugaaggugg ucgacgagcu ggugaagguc augggacggc acaaaccgga gaauaucgug     2280

auugaaaugg cccgagaaaa ccagacuacc cagaagggcc agaaaaacuc ccgcgaaagg     2340

augaagcgga ucgaagaagg aaucaaggag cugggcagcc agauccugaa agagcacccg     2400

guggaaaaca cgcagcugca gaacgagaag cucuaccugu acuauuugca aaauggacgg     2460

gacauguacg uggaccaaga gcuggacauc aaucgguugu cugauuacga cguggaccac     2520

aucguuccac aguccuuucu gaaggaugac ucgaucgaua acaagguguu gacucgcagc     2580

gacaagaaca gagggaaguc agauaaugug ccaucggagg aggucgugaa gaagaugaag     2640

aauuacuggc ggcagcuccu gaaugcgaag cugauuaccc agagaaaguu ugacaaucuc     2700

acuaaagccg agcgcggcgg acucucagag cuggauaagg cuggauucau caaacggcag     2760

cuggucgaga cucggcagau uaccaagcac guggcgcaga ucuuggacuc ccgcaugaac     2820

acuaaauacg acgagaacga uaagcucauc cgggaaguga aggugauuac ccugaaaagc     2880

aaacuugugu cggacuuucg gaaggacuuu caguuuuaca aagugagaga aaucaacaac     2940

uaccaucacg cgcaugacgc auaccucaac gcuguggucg guaccgcccu gaucaaaaag     3000

uacccuaaac uugaaucgga guuuguguac ggagacuaca aggucuacga cgugaggaag     3060

augauagcca aguccgaaca ggaaaucggg aaagcaacug cgaaauacuu cuuuuacuca     3120

aacaucauga acuuuuucaa gacugaaauu acgcuggcca auggagaaau caggaagagg     3180

ccacugaucg aaacuaacgg agaaacgggc gaaaucgugu gggacaaggg cagggacuuc     3240

gcaacuguuc gcaaagugcu cucuaugccg caagucaaua uugugaagaa aaccgaagug     3300

caaaccggcg gauuuucaaa ggaaucgauc cucccaaaga gaaauagcga caagcucauu     3360

gcacgcaaga aagacuggga cccgaagaag uacggaggau ucgauucgcc gacugucgca     3420

uacuccgucc ucgugguggc caagguggag aagggaaaga gcaaaaagcu caaauccguc     3480

aaagagcugc uggggauuac caucauggaa cgauccucgu ucgagaagaa cccgauugau     3540

uuccucgagg cgaaggguua caaggaggug aagaaggauc ugaucaucaa acuccccaag     3600

uacucacugu ucgaacugga aaauggucgg aagcgcaugc uggcuucggc cggagaacuc     3660

caaaaaggaa augagcuggc cuugccuagc aaguacguca acuuccucua ucuugcuucg     3720

cacuacgaaa aacucaaagg gucaccggaa gauaacgaac agaagcagcu uuucguggag     3780

cagcacaagc auuaucugga ugaaaucauc gaacaaaucu ccgaguuuuc aaagcgcgug     3840

auccucgccg acgccaaccu cgacaaaguc cugucggccu acaauaagca uagagauaag     3900

ccgaucagag aacaggccga gaacauuauc cacuuguuca cccugacuaa ccugggagcc     3960

ccagccgccu ucaaguacuu cgauacuacu aucgaucgca aaagauacac guccaccaag     4020

gaaguucugg acgcgacccu gauccaccaa agcaucacug gacucuacga aacuaggauc     4080

gaucugucgc agcugggugg cgauggcggu ggaucuccga aaaagaagag aaagguguaa     4140

uga                                                                   4143


<210>  206

<400>  206
000

<210>  207

<400>  207
000

<210>  208

<400>  208
000

<210>  209

<400>  209
000

<210>  210

<400>  210
000

<210>  211

<400>  211
000

<210>  212

<400>  212
000

<210>  213
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence of Cas9 (without NLS)

<400>  213

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  214

<400>  214
000

<210>  215

<400>  215
000

<210>  216

<400>  216
000

<210>  217

<400>  217
000

<210>  218

<400>  218
000

<210>  219

<400>  219
000

<210>  220

<400>  220
000

<210>  221

<400>  221
000

<210>  222
<211>  1392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence of Cas9 with two nuclear 
       localization signals as the C-terminal amino acids

<400>  222

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Gly Ser  Gly Ser Pro Lys Lys  Lys Arg Lys Val Asp  Gly Ser Pro 
    1370                 1375                 1380             


Lys Lys  Lys Arg Lys Val Asp  Ser Gly 
    1385                 1390         


<210>  223

<400>  223
000

<210>  224

<400>  224
000

<210>  225

<400>  225
000

<210>  226

<400>  226
000

<210>  227

<400>  227
000

<210>  228

<400>  228
000

<210>  229

<400>  229
000

<210>  230

<400>  230
000

<210>  231
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: T7 promoter

<400>  231
taatacgact cactata                                                      17


<210>  232
<211>  50
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Human beta-globin 5 UTR

<400>  232
acatttgctt ctgacacaac tgtgttcact agcaacctca aacagacacc                  50


<210>  233
<211>  132
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Human beta-globin 3 UTR

<400>  233
gctcgctttc ttgctgtcca atttctatta aaggttcctt tgttccctaa gtccaactac       60

taaactgggg gatattatga agggccttga gcatctggat tctgcctaat aaaaaacatt      120

tattttcatt gc                                                          132


<210>  234
<211>  66
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Human alpha-globin 5 UTR

<400>  234
cataaaccct ggcgcgctcg cggcccggca ctcttctggt ccccacagac tcagagagaa       60

cccacc                                                                  66


<210>  235
<211>  110
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Human alpha-globin 3 UTR

<400>  235
gctggagcct cggtggccat gcttcttgcc ccttgggcct ccccccagcc cctcctcccc       60

ttcctgcacc cgtacccccg tggtctttga ataaagtctg agtgggcggc                 110


<210>  236
<211>  29
<212>  DNA
<213>  Xenopus laevis


<220>
<221>  misc_feature
<223>  Xenopus laevis beta-globin 5 UTR

<400>  236
aagctcagaa taaacgctca actttggcc                                         29


<210>  237
<211>  130
<212>  DNA
<213>  Xenopus laevis


<220>
<221>  misc_feature
<223>  Xenopus laevis beta-globin 3 UTR

<400>  237
accagcctca agaacacccg aatggagtct ctaagctaca taataccaac ttacacttta       60

caaaatgttg tcccccaaaa tgtagccatt cgtatctgct cctaataaaa agaaagtttc      120

ttcacattct                                                             130


<210>  238
<211>  27
<212>  DNA
<213>  Bos sp.


<220>
<221>  misc_feature
<223>  Bovine Growth Hormone 5 UTR

<400>  238
cagggtcctg tggacagctc accagct                                           27


<210>  239
<211>  102
<212>  DNA
<213>  Bos sp.


<220>
<221>  misc_feature
<223>  Bovine Growth Hormone 3 UTR

<400>  239
ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac       60

tcccactgtc ctttcctaat aaaatgagga aattgcatcg ca                         102


<210>  240
<211>  93
<212>  DNA
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  Mus musculus hemoglobin alpha, adult chain 1 (Hba-a1), 3UTR

<400>  240
gctgccttct gcggggcttg ccttctggcc atgcccttct tctctccctt gcacctgtac       60

ctcttggtct ttgaataaag cctgagtagg aag                                    93


<210>  241
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: HSD17B4 5 UTR

<400>  241
tcccgcagtc ggcgtccagc ggctctgctt gttcgtgtgt gtgtcgttgc aggccttatt       60

c                                                                       61


<210>  242
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G282 guide RNA targeting TTR


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  242
uuacagccac gucuacagca guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  243
<211>  4411
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript with 5 UTR of HSD, ORF corresponding 
       to SEQ ID NO: 204, Kozak sequence, and 3 UTR of ALB

<400>  243
gggtcccgca gtcggcgtcc agcggctctg cttgttcgtg tgtgtgtcgt tgcaggcctt       60

attcggatcc gccaccatgg acaagaagta cagcatcgga ctggacatcg gaacaaacag      120

cgtcggatgg gcagtcatca cagacgaata caaggtcccg agcaagaagt tcaaggtcct      180

gggaaacaca gacagacaca gcatcaagaa gaacctgatc ggagcactgc tgttcgacag      240

cggagaaaca gcagaagcaa caagactgaa gagaacagca agaagaagat acacaagaag      300

aaagaacaga atctgctacc tgcaggaaat cttcagcaac gaaatggcaa aggtcgacga      360

cagcttcttc cacagactgg aagaaagctt cctggtcgaa gaagacaaga agcacgaaag      420

acacccgatc ttcggaaaca tcgtcgacga agtcgcatac cacgaaaagt acccgacaat      480

ctaccacctg agaaagaagc tggtcgacag cacagacaag gcagacctga gactgatcta      540

cctggcactg gcacacatga tcaagttcag aggacacttc ctgatcgaag gagacctgaa      600

cccggacaac agcgacgtcg acaagctgtt catccagctg gtccagacat acaaccagct      660

gttcgaagaa aacccgatca acgcaagcgg agtcgacgca aaggcaatcc tgagcgcaag      720

actgagcaag agcagaagac tggaaaacct gatcgcacag ctgccgggag aaaagaagaa      780

cggactgttc ggaaacctga tcgcactgag cctgggactg acaccgaact tcaagagcaa      840

cttcgacctg gcagaagacg caaagctgca gctgagcaag gacacatacg acgacgacct      900

ggacaacctg ctggcacaga tcggagacca gtacgcagac ctgttcctgg cagcaaagaa      960

cctgagcgac gcaatcctgc tgagcgacat cctgagagtc aacacagaaa tcacaaaggc     1020

accgctgagc gcaagcatga tcaagagata cgacgaacac caccaggacc tgacactgct     1080

gaaggcactg gtcagacagc agctgccgga aaagtacaag gaaatcttct tcgaccagag     1140

caagaacgga tacgcaggat acatcgacgg aggagcaagc caggaagaat tctacaagtt     1200

catcaagccg atcctggaaa agatggacgg aacagaagaa ctgctggtca agctgaacag     1260

agaagacctg ctgagaaagc agagaacatt cgacaacgga agcatcccgc accagatcca     1320

cctgggagaa ctgcacgcaa tcctgagaag acaggaagac ttctacccgt tcctgaagga     1380

caacagagaa aagatcgaaa agatcctgac attcagaatc ccgtactacg tcggaccgct     1440

ggcaagagga aacagcagat tcgcatggat gacaagaaag agcgaagaaa caatcacacc     1500

gtggaacttc gaagaagtcg tcgacaaggg agcaagcgca cagagcttca tcgaaagaat     1560

gacaaacttc gacaagaacc tgccgaacga aaaggtcctg ccgaagcaca gcctgctgta     1620

cgaatacttc acagtctaca acgaactgac aaaggtcaag tacgtcacag aaggaatgag     1680

aaagccggca ttcctgagcg gagaacagaa gaaggcaatc gtcgacctgc tgttcaagac     1740

aaacagaaag gtcacagtca agcagctgaa ggaagactac ttcaagaaga tcgaatgctt     1800

cgacagcgtc gaaatcagcg gagtcgaaga cagattcaac gcaagcctgg gaacatacca     1860

cgacctgctg aagatcatca aggacaagga cttcctggac aacgaagaaa acgaagacat     1920

cctggaagac atcgtcctga cactgacact gttcgaagac agagaaatga tcgaagaaag     1980

actgaagaca tacgcacacc tgttcgacga caaggtcatg aagcagctga agagaagaag     2040

atacacagga tggggaagac tgagcagaaa gctgatcaac ggaatcagag acaagcagag     2100

cggaaagaca atcctggact tcctgaagag cgacggattc gcaaacagaa acttcatgca     2160

gctgatccac gacgacagcc tgacattcaa ggaagacatc cagaaggcac aggtcagcgg     2220

acagggagac agcctgcacg aacacatcgc aaacctggca ggaagcccgg caatcaagaa     2280

gggaatcctg cagacagtca aggtcgtcga cgaactggtc aaggtcatgg gaagacacaa     2340

gccggaaaac atcgtcatcg aaatggcaag agaaaaccag acaacacaga agggacagaa     2400

gaacagcaga gaaagaatga agagaatcga agaaggaatc aaggaactgg gaagccagat     2460

cctgaaggaa cacccggtcg aaaacacaca gctgcagaac gaaaagctgt acctgtacta     2520

cctgcagaac ggaagagaca tgtacgtcga ccaggaactg gacatcaaca gactgagcga     2580

ctacgacgtc gaccacatcg tcccgcagag cttcctgaag gacgacagca tcgacaacaa     2640

ggtcctgaca agaagcgaca agaacagagg aaagagcgac aacgtcccga gcgaagaagt     2700

cgtcaagaag atgaagaact actggagaca gctgctgaac gcaaagctga tcacacagag     2760

aaagttcgac aacctgacaa aggcagagag aggaggactg agcgaactgg acaaggcagg     2820

attcatcaag agacagctgg tcgaaacaag acagatcaca aagcacgtcg cacagatcct     2880

ggacagcaga atgaacacaa agtacgacga aaacgacaag ctgatcagag aagtcaaggt     2940

catcacactg aagagcaagc tggtcagcga cttcagaaag gacttccagt tctacaaggt     3000

cagagaaatc aacaactacc accacgcaca cgacgcatac ctgaacgcag tcgtcggaac     3060

agcactgatc aagaagtacc cgaagctgga aagcgaattc gtctacggag actacaaggt     3120

ctacgacgtc agaaagatga tcgcaaagag cgaacaggaa atcggaaagg caacagcaaa     3180

gtacttcttc tacagcaaca tcatgaactt cttcaagaca gaaatcacac tggcaaacgg     3240

agaaatcaga aagagaccgc tgatcgaaac aaacggagaa acaggagaaa tcgtctggga     3300

caagggaaga gacttcgcaa cagtcagaaa ggtcctgagc atgccgcagg tcaacatcgt     3360

caagaagaca gaagtccaga caggaggatt cagcaaggaa agcatcctgc cgaagagaaa     3420

cagcgacaag ctgatcgcaa gaaagaagga ctgggacccg aagaagtacg gaggattcga     3480

cagcccgaca gtcgcataca gcgtcctggt cgtcgcaaag gtcgaaaagg gaaagagcaa     3540

gaagctgaag agcgtcaagg aactgctggg aatcacaatc atggaaagaa gcagcttcga     3600

aaagaacccg atcgacttcc tggaagcaaa gggatacaag gaagtcaaga aggacctgat     3660

catcaagctg ccgaagtaca gcctgttcga actggaaaac ggaagaaaga gaatgctggc     3720

aagcgcagga gaactgcaga agggaaacga actggcactg ccgagcaagt acgtcaactt     3780

cctgtacctg gcaagccact acgaaaagct gaagggaagc ccggaagaca acgaacagaa     3840

gcagctgttc gtcgaacagc acaagcacta cctggacgaa atcatcgaac agatcagcga     3900

attcagcaag agagtcatcc tggcagacgc aaacctggac aaggtcctga gcgcatacaa     3960

caagcacaga gacaagccga tcagagaaca ggcagaaaac atcatccacc tgttcacact     4020

gacaaacctg ggagcaccgg cagcattcaa gtacttcgac acaacaatcg acagaaagag     4080

atacacaagc acaaaggaag tcctggacgc aacactgatc caccagagca tcacaggact     4140

gtacgaaaca agaatcgacc tgagccagct gggaggagac ggaggaggaa gcccgaagaa     4200

gaagagaaag gtctagctag ccatcacatt taaaagcatc tcagcctacc atgagaataa     4260

gagaaagaaa atgaagatca atagcttatt catctctttt tctttttcgt tggtgtaaag     4320

ccaacaccct gtctaaaaaa cataaatttc tttaatcatt ttgcctcttt tctctgtgct     4380

tcaattaata aaaaatggaa agaacctcga g                                    4411


<210>  244
<211>  4405
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript with 5 UTR of HSD, ORF corresponding 
       to SEQ ID NO: 204, and 3 UTR of ALB

<400>  244
gggtcccgca gtcggcgtcc agcggctctg cttgttcgtg tgtgtgtcgt tgcaggcctt       60

attcggatcc atggacaaga agtacagcat cggactggac atcggaacaa acagcgtcgg      120

atgggcagtc atcacagacg aatacaaggt cccgagcaag aagttcaagg tcctgggaaa      180

cacagacaga cacagcatca agaagaacct gatcggagca ctgctgttcg acagcggaga      240

aacagcagaa gcaacaagac tgaagagaac agcaagaaga agatacacaa gaagaaagaa      300

cagaatctgc tacctgcagg aaatcttcag caacgaaatg gcaaaggtcg acgacagctt      360

cttccacaga ctggaagaaa gcttcctggt cgaagaagac aagaagcacg aaagacaccc      420

gatcttcgga aacatcgtcg acgaagtcgc ataccacgaa aagtacccga caatctacca      480

cctgagaaag aagctggtcg acagcacaga caaggcagac ctgagactga tctacctggc      540

actggcacac atgatcaagt tcagaggaca cttcctgatc gaaggagacc tgaacccgga      600

caacagcgac gtcgacaagc tgttcatcca gctggtccag acatacaacc agctgttcga      660

agaaaacccg atcaacgcaa gcggagtcga cgcaaaggca atcctgagcg caagactgag      720

caagagcaga agactggaaa acctgatcgc acagctgccg ggagaaaaga agaacggact      780

gttcggaaac ctgatcgcac tgagcctggg actgacaccg aacttcaaga gcaacttcga      840

cctggcagaa gacgcaaagc tgcagctgag caaggacaca tacgacgacg acctggacaa      900

cctgctggca cagatcggag accagtacgc agacctgttc ctggcagcaa agaacctgag      960

cgacgcaatc ctgctgagcg acatcctgag agtcaacaca gaaatcacaa aggcaccgct     1020

gagcgcaagc atgatcaaga gatacgacga acaccaccag gacctgacac tgctgaaggc     1080

actggtcaga cagcagctgc cggaaaagta caaggaaatc ttcttcgacc agagcaagaa     1140

cggatacgca ggatacatcg acggaggagc aagccaggaa gaattctaca agttcatcaa     1200

gccgatcctg gaaaagatgg acggaacaga agaactgctg gtcaagctga acagagaaga     1260

cctgctgaga aagcagagaa cattcgacaa cggaagcatc ccgcaccaga tccacctggg     1320

agaactgcac gcaatcctga gaagacagga agacttctac ccgttcctga aggacaacag     1380

agaaaagatc gaaaagatcc tgacattcag aatcccgtac tacgtcggac cgctggcaag     1440

aggaaacagc agattcgcat ggatgacaag aaagagcgaa gaaacaatca caccgtggaa     1500

cttcgaagaa gtcgtcgaca agggagcaag cgcacagagc ttcatcgaaa gaatgacaaa     1560

cttcgacaag aacctgccga acgaaaaggt cctgccgaag cacagcctgc tgtacgaata     1620

cttcacagtc tacaacgaac tgacaaaggt caagtacgtc acagaaggaa tgagaaagcc     1680

ggcattcctg agcggagaac agaagaaggc aatcgtcgac ctgctgttca agacaaacag     1740

aaaggtcaca gtcaagcagc tgaaggaaga ctacttcaag aagatcgaat gcttcgacag     1800

cgtcgaaatc agcggagtcg aagacagatt caacgcaagc ctgggaacat accacgacct     1860

gctgaagatc atcaaggaca aggacttcct ggacaacgaa gaaaacgaag acatcctgga     1920

agacatcgtc ctgacactga cactgttcga agacagagaa atgatcgaag aaagactgaa     1980

gacatacgca cacctgttcg acgacaaggt catgaagcag ctgaagagaa gaagatacac     2040

aggatgggga agactgagca gaaagctgat caacggaatc agagacaagc agagcggaaa     2100

gacaatcctg gacttcctga agagcgacgg attcgcaaac agaaacttca tgcagctgat     2160

ccacgacgac agcctgacat tcaaggaaga catccagaag gcacaggtca gcggacaggg     2220

agacagcctg cacgaacaca tcgcaaacct ggcaggaagc ccggcaatca agaagggaat     2280

cctgcagaca gtcaaggtcg tcgacgaact ggtcaaggtc atgggaagac acaagccgga     2340

aaacatcgtc atcgaaatgg caagagaaaa ccagacaaca cagaagggac agaagaacag     2400

cagagaaaga atgaagagaa tcgaagaagg aatcaaggaa ctgggaagcc agatcctgaa     2460

ggaacacccg gtcgaaaaca cacagctgca gaacgaaaag ctgtacctgt actacctgca     2520

gaacggaaga gacatgtacg tcgaccagga actggacatc aacagactga gcgactacga     2580

cgtcgaccac atcgtcccgc agagcttcct gaaggacgac agcatcgaca acaaggtcct     2640

gacaagaagc gacaagaaca gaggaaagag cgacaacgtc ccgagcgaag aagtcgtcaa     2700

gaagatgaag aactactgga gacagctgct gaacgcaaag ctgatcacac agagaaagtt     2760

cgacaacctg acaaaggcag agagaggagg actgagcgaa ctggacaagg caggattcat     2820

caagagacag ctggtcgaaa caagacagat cacaaagcac gtcgcacaga tcctggacag     2880

cagaatgaac acaaagtacg acgaaaacga caagctgatc agagaagtca aggtcatcac     2940

actgaagagc aagctggtca gcgacttcag aaaggacttc cagttctaca aggtcagaga     3000

aatcaacaac taccaccacg cacacgacgc atacctgaac gcagtcgtcg gaacagcact     3060

gatcaagaag tacccgaagc tggaaagcga attcgtctac ggagactaca aggtctacga     3120

cgtcagaaag atgatcgcaa agagcgaaca ggaaatcgga aaggcaacag caaagtactt     3180

cttctacagc aacatcatga acttcttcaa gacagaaatc acactggcaa acggagaaat     3240

cagaaagaga ccgctgatcg aaacaaacgg agaaacagga gaaatcgtct gggacaaggg     3300

aagagacttc gcaacagtca gaaaggtcct gagcatgccg caggtcaaca tcgtcaagaa     3360

gacagaagtc cagacaggag gattcagcaa ggaaagcatc ctgccgaaga gaaacagcga     3420

caagctgatc gcaagaaaga aggactggga cccgaagaag tacggaggat tcgacagccc     3480

gacagtcgca tacagcgtcc tggtcgtcgc aaaggtcgaa aagggaaaga gcaagaagct     3540

gaagagcgtc aaggaactgc tgggaatcac aatcatggaa agaagcagct tcgaaaagaa     3600

cccgatcgac ttcctggaag caaagggata caaggaagtc aagaaggacc tgatcatcaa     3660

gctgccgaag tacagcctgt tcgaactgga aaacggaaga aagagaatgc tggcaagcgc     3720

aggagaactg cagaagggaa acgaactggc actgccgagc aagtacgtca acttcctgta     3780

cctggcaagc cactacgaaa agctgaaggg aagcccggaa gacaacgaac agaagcagct     3840

gttcgtcgaa cagcacaagc actacctgga cgaaatcatc gaacagatca gcgaattcag     3900

caagagagtc atcctggcag acgcaaacct ggacaaggtc ctgagcgcat acaacaagca     3960

cagagacaag ccgatcagag aacaggcaga aaacatcatc cacctgttca cactgacaaa     4020

cctgggagca ccggcagcat tcaagtactt cgacacaaca atcgacagaa agagatacac     4080

aagcacaaag gaagtcctgg acgcaacact gatccaccag agcatcacag gactgtacga     4140

aacaagaatc gacctgagcc agctgggagg agacggagga ggaagcccga agaagaagag     4200

aaaggtctag ctagccatca catttaaaag catctcagcc taccatgaga ataagagaaa     4260

gaaaatgaag atcaatagct tattcatctc tttttctttt tcgttggtgt aaagccaaca     4320

ccctgtctaa aaaacataaa tttctttaat cattttgcct cttttctctg tgcttcaatt     4380

aataaaaaat ggaaagaacc tcgag                                           4405


<210>  245

<400>  245
000

<210>  246
<211>  4459
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript with 5 UTR of HSD, ORF corresponding 
       to SEQ ID NO: 245, Kozak sequence, and 3 UTR of ALB

<400>  246
gggtcccgca gtcggcgtcc agcggctctg cttgttcgtg tgtgtgtcgt tgcaggcctt       60

attcggatct gccaccatgg ataagaagta ctcgatcggg ctggatatcg gaactaattc      120

cgtgggttgg gcagtgatca cggatgaata caaagtgccg tccaagaagt tcaaggtcct      180

ggggaacacc gatagacaca gcatcaagaa gaatctcatc ggagccctgc tgtttgactc      240

cggcgaaacc gcagaagcga cccggctcaa acgtaccgcg aggcgacgct acacccggcg      300

gaagaatcgc atctgctatc tgcaagaaat cttttcgaac gaaatggcaa aggtggacga      360

cagcttcttc caccgcctgg aagaatcttt cctggtggag gaggacaaga agcatgaacg      420

gcatcctatc tttggaaaca tcgtggacga agtggcgtac cacgaaaagt acccgaccat      480

ctaccatctg cggaagaagt tggttgactc aactgacaag gccgacctca gattgatcta      540

cttggccctc gcccatatga tcaaattccg cggacacttc ctgatcgaag gcgatctgaa      600

ccctgataac tccgacgtgg ataagctgtt cattcaactg gtgcagacct acaaccaact      660

gttcgaagaa aacccaatca atgccagcgg cgtcgatgcc aaggccatcc tgtccgcccg      720

gctgtcgaag tcgcggcgcc tcgaaaacct gatcgcacag ctgccgggag agaagaagaa      780

cggacttttc ggcaacttga tcgctctctc actgggactc actcccaatt tcaagtccaa      840

ttttgacctg gccgaggacg cgaagctgca actctcaaag gacacctacg acgacgactt      900

ggacaatttg ctggcacaaa ttggcgatca gtacgcggat ctgttccttg ccgctaagaa      960

cctttcggac gcaatcttgc tgtccgatat cctgcgcgtg aacaccgaaa taaccaaagc     1020

gccgcttagc gcctcgatga ttaagcggta cgacgagcat caccaggatc tcacgctgct     1080

caaagcgctc gtgagacagc aactgcctga aaagtacaag gagattttct tcgaccagtc     1140

caagaatggg tacgcagggt acatcgatgg aggcgccagc caggaagagt tctataagtt     1200

catcaagcca atcctggaaa agatggacgg aaccgaagaa ctgctggtca agctgaacag     1260

ggaggatctg ctccgcaaac agagaacctt tgacaacgga agcattccac accagatcca     1320

tctgggtgag ctgcacgcca tcttgcggcg ccaggaggac ttttacccat tcctcaagga     1380

caaccgggaa aagatcgaga aaattctgac gttccgcatc ccgtattacg tgggcccact     1440

ggcgcgcggc aattcgcgct tcgcgtggat gactagaaaa tcagaggaaa ccatcactcc     1500

ttggaatttc gaggaagttg tggataaggg agcttcggca caatccttca tcgaacgaat     1560

gaccaacttc gacaagaatc tcccaaacga gaaggtgctt cctaagcaca gcctccttta     1620

cgaatacttc actgtctaca acgaactgac taaagtgaaa tacgttactg aaggaatgag     1680

gaagccggcc tttctgagcg gagaacagaa gaaagcgatt gtcgatctgc tgttcaagac     1740

caaccgcaag gtgaccgtca agcagcttaa agaggactac ttcaagaaga tcgagtgttt     1800

cgactcagtg gaaatcagcg gagtggagga cagattcaac gcttcgctgg gaacctatca     1860

tgatctcctg aagatcatca aggacaagga cttccttgac aacgaggaga acgaggacat     1920

cctggaagat atcgtcctga ccttgaccct tttcgaggat cgcgagatga tcgaggagag     1980

gcttaagacc tacgctcatc tcttcgacga taaggtcatg aaacaactca agcgccgccg     2040

gtacactggt tggggccgcc tctcccgcaa gctgatcaac ggtattcgcg ataaacagag     2100

cggtaaaact atcctggatt tcctcaaatc ggatggcttc gctaatcgta acttcatgca     2160

gttgatccac gacgacagcc tgacctttaa ggaggacatc cagaaagcac aagtgagcgg     2220

acagggagac tcactccatg aacacatcgc gaatctggcc ggttcgccgg cgattaagaa     2280

gggaatcctg caaactgtga aggtggtgga cgagctggtg aaggtcatgg gacggcacaa     2340

accggagaat atcgtgattg aaatggcccg agaaaaccag actacccaga agggccagaa     2400

gaactcccgc gaaaggatga agcggatcga agaaggaatc aaggagctgg gcagccagat     2460

cctgaaagag cacccggtgg aaaacacgca gctgcagaac gagaagctct acctgtacta     2520

tttgcaaaat ggacgggaca tgtacgtgga ccaagagctg gacatcaatc ggttgtctga     2580

ttacgacgtg gaccacatcg ttccacagtc ctttctgaag gatgactcca tcgataacaa     2640

ggtgttgact cgcagcgaca agaacagagg gaagtcagat aatgtgccat cggaggaggt     2700

cgtgaagaag atgaagaatt actggcggca gctcctgaat gcgaagctga ttacccagag     2760

aaagtttgac aatctcacta aagccgagcg cggcggactc tcagagctgg ataaggctgg     2820

attcatcaaa cggcagctgg tcgagactcg gcagattacc aagcacgtgg cgcagatcct     2880

ggactcccgc atgaacacta aatacgacga gaacgataag ctcatccggg aagtgaaggt     2940

gattaccctg aaaagcaaac ttgtgtcgga ctttcggaag gactttcagt tttacaaagt     3000

gagagaaatc aacaactacc atcacgcgca tgacgcatac ctcaacgctg tggtcggcac     3060

cgccctgatc aagaagtacc ctaaacttga atcggagttt gtgtacggag actacaaggt     3120

ctacgacgtg aggaagatga tagccaagtc cgaacaggaa atcgggaaag caactgcgaa     3180

atacttcttt tactcaaaca tcatgaactt cttcaagact gaaattacgc tggccaatgg     3240

agaaatcagg aagaggccac tgatcgaaac taacggagaa acgggcgaaa tcgtgtggga     3300

caagggcagg gacttcgcaa ctgttcgcaa agtgctctct atgccgcaag tcaatattgt     3360

gaagaaaacc gaagtgcaaa ccggcggatt ttcaaaggaa tcgatcctcc caaagagaaa     3420

tagcgacaag ctcattgcac gcaagaaaga ctgggacccg aagaagtacg gaggattcga     3480

ttcgccgact gtcgcatact ccgtcctcgt ggtggccaag gtggagaagg gaaagagcaa     3540

gaagctcaaa tccgtcaaag agctgctggg gattaccatc atggaacgat cctcgttcga     3600

gaagaacccg attgatttcc tggaggcgaa gggttacaag gaggtgaaga aggatctgat     3660

catcaaactg cccaagtact cactgttcga actggaaaat ggtcggaagc gcatgctggc     3720

ttcggccgga gaactccaga aaggaaatga gctggccttg cctagcaagt acgtcaactt     3780

cctctatctt gcttcgcact acgagaaact caaagggtca ccggaagata acgaacagaa     3840

gcagcttttc gtggagcagc acaagcatta tctggatgaa atcatcgaac aaatctccga     3900

gttttcaaag cgcgtgatcc tcgccgacgc caacctcgac aaagtcctgt cggcctacaa     3960

taagcataga gataagccga tcagagaaca ggccgagaac attatccact tgttcaccct     4020

gactaacctg ggagctccag ccgccttcaa gtacttcgat actactatcg accgcaaaag     4080

atacacgtcc accaaggaag ttctggacgc gaccctgatc caccaaagca tcactggact     4140

ctacgaaact aggatcgatc tgtcgcagct gggtggcgat ggtggcggtg gatcctaccc     4200

atacgacgtg cctgactacg cctccggagg tggtggcccc aagaagaaac ggaaggtgtg     4260

atagctagcc atcacattta aaagcatctc agcctaccat gagaataaga gaaagaaaat     4320

gaagatcaat agcttattca tctctttttc tttttcgttg gtgtaaagcc aacaccctgt     4380

ctaaaaaaca taaatttctt taatcatttt gcctcttttc tctgtgcttc aattaataaa     4440

aaatggaaag aacctcgag                                                  4459


<210>  247
<211>  4453
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript with 5 UTR of HSD, ORF corresponding 
       to SEQ ID NO: 245, and 3 UTR of ALB

<400>  247
gggtcccgca gtcggcgtcc agcggctctg cttgttcgtg tgtgtgtcgt tgcaggcctt       60

attcggatct atggataaga agtactcgat cgggctggat atcggaacta attccgtggg      120

ttgggcagtg atcacggatg aatacaaagt gccgtccaag aagttcaagg tcctggggaa      180

caccgataga cacagcatca agaagaatct catcggagcc ctgctgtttg actccggcga      240

aaccgcagaa gcgacccggc tcaaacgtac cgcgaggcga cgctacaccc ggcggaagaa      300

tcgcatctgc tatctgcaag aaatcttttc gaacgaaatg gcaaaggtgg acgacagctt      360

cttccaccgc ctggaagaat ctttcctggt ggaggaggac aagaagcatg aacggcatcc      420

tatctttgga aacatcgtgg acgaagtggc gtaccacgaa aagtacccga ccatctacca      480

tctgcggaag aagttggttg actcaactga caaggccgac ctcagattga tctacttggc      540

cctcgcccat atgatcaaat tccgcggaca cttcctgatc gaaggcgatc tgaaccctga      600

taactccgac gtggataagc tgttcattca actggtgcag acctacaacc aactgttcga      660

agaaaaccca atcaatgcca gcggcgtcga tgccaaggcc atcctgtccg cccggctgtc      720

gaagtcgcgg cgcctcgaaa acctgatcgc acagctgccg ggagagaaga agaacggact      780

tttcggcaac ttgatcgctc tctcactggg actcactccc aatttcaagt ccaattttga      840

cctggccgag gacgcgaagc tgcaactctc aaaggacacc tacgacgacg acttggacaa      900

tttgctggca caaattggcg atcagtacgc ggatctgttc cttgccgcta agaacctttc      960

ggacgcaatc ttgctgtccg atatcctgcg cgtgaacacc gaaataacca aagcgccgct     1020

tagcgcctcg atgattaagc ggtacgacga gcatcaccag gatctcacgc tgctcaaagc     1080

gctcgtgaga cagcaactgc ctgaaaagta caaggagatt ttcttcgacc agtccaagaa     1140

tgggtacgca gggtacatcg atggaggcgc cagccaggaa gagttctata agttcatcaa     1200

gccaatcctg gaaaagatgg acggaaccga agaactgctg gtcaagctga acagggagga     1260

tctgctccgc aaacagagaa cctttgacaa cggaagcatt ccacaccaga tccatctggg     1320

tgagctgcac gccatcttgc ggcgccagga ggacttttac ccattcctca aggacaaccg     1380

ggaaaagatc gagaaaattc tgacgttccg catcccgtat tacgtgggcc cactggcgcg     1440

cggcaattcg cgcttcgcgt ggatgactag aaaatcagag gaaaccatca ctccttggaa     1500

tttcgaggaa gttgtggata agggagcttc ggcacaatcc ttcatcgaac gaatgaccaa     1560

cttcgacaag aatctcccaa acgagaaggt gcttcctaag cacagcctcc tttacgaata     1620

cttcactgtc tacaacgaac tgactaaagt gaaatacgtt actgaaggaa tgaggaagcc     1680

ggcctttctg agcggagaac agaagaaagc gattgtcgat ctgctgttca agaccaaccg     1740

caaggtgacc gtcaagcagc ttaaagagga ctacttcaag aagatcgagt gtttcgactc     1800

agtggaaatc agcggagtgg aggacagatt caacgcttcg ctgggaacct atcatgatct     1860

cctgaagatc atcaaggaca aggacttcct tgacaacgag gagaacgagg acatcctgga     1920

agatatcgtc ctgaccttga cccttttcga ggatcgcgag atgatcgagg agaggcttaa     1980

gacctacgct catctcttcg acgataaggt catgaaacaa ctcaagcgcc gccggtacac     2040

tggttggggc cgcctctccc gcaagctgat caacggtatt cgcgataaac agagcggtaa     2100

aactatcctg gatttcctca aatcggatgg cttcgctaat cgtaacttca tgcagttgat     2160

ccacgacgac agcctgacct ttaaggagga catccagaaa gcacaagtga gcggacaggg     2220

agactcactc catgaacaca tcgcgaatct ggccggttcg ccggcgatta agaagggaat     2280

cctgcaaact gtgaaggtgg tggacgagct ggtgaaggtc atgggacggc acaaaccgga     2340

gaatatcgtg attgaaatgg cccgagaaaa ccagactacc cagaagggcc agaagaactc     2400

ccgcgaaagg atgaagcgga tcgaagaagg aatcaaggag ctgggcagcc agatcctgaa     2460

agagcacccg gtggaaaaca cgcagctgca gaacgagaag ctctacctgt actatttgca     2520

aaatggacgg gacatgtacg tggaccaaga gctggacatc aatcggttgt ctgattacga     2580

cgtggaccac atcgttccac agtcctttct gaaggatgac tccatcgata acaaggtgtt     2640

gactcgcagc gacaagaaca gagggaagtc agataatgtg ccatcggagg aggtcgtgaa     2700

gaagatgaag aattactggc ggcagctcct gaatgcgaag ctgattaccc agagaaagtt     2760

tgacaatctc actaaagccg agcgcggcgg actctcagag ctggataagg ctggattcat     2820

caaacggcag ctggtcgaga ctcggcagat taccaagcac gtggcgcaga tcctggactc     2880

ccgcatgaac actaaatacg acgagaacga taagctcatc cgggaagtga aggtgattac     2940

cctgaaaagc aaacttgtgt cggactttcg gaaggacttt cagttttaca aagtgagaga     3000

aatcaacaac taccatcacg cgcatgacgc atacctcaac gctgtggtcg gcaccgccct     3060

gatcaagaag taccctaaac ttgaatcgga gtttgtgtac ggagactaca aggtctacga     3120

cgtgaggaag atgatagcca agtccgaaca ggaaatcggg aaagcaactg cgaaatactt     3180

cttttactca aacatcatga acttcttcaa gactgaaatt acgctggcca atggagaaat     3240

caggaagagg ccactgatcg aaactaacgg agaaacgggc gaaatcgtgt gggacaaggg     3300

cagggacttc gcaactgttc gcaaagtgct ctctatgccg caagtcaata ttgtgaagaa     3360

aaccgaagtg caaaccggcg gattttcaaa ggaatcgatc ctcccaaaga gaaatagcga     3420

caagctcatt gcacgcaaga aagactggga cccgaagaag tacggaggat tcgattcgcc     3480

gactgtcgca tactccgtcc tcgtggtggc caaggtggag aagggaaaga gcaagaagct     3540

caaatccgtc aaagagctgc tggggattac catcatggaa cgatcctcgt tcgagaagaa     3600

cccgattgat ttcctggagg cgaagggtta caaggaggtg aagaaggatc tgatcatcaa     3660

actgcccaag tactcactgt tcgaactgga aaatggtcgg aagcgcatgc tggcttcggc     3720

cggagaactc cagaaaggaa atgagctggc cttgcctagc aagtacgtca acttcctcta     3780

tcttgcttcg cactacgaga aactcaaagg gtcaccggaa gataacgaac agaagcagct     3840

tttcgtggag cagcacaagc attatctgga tgaaatcatc gaacaaatct ccgagttttc     3900

aaagcgcgtg atcctcgccg acgccaacct cgacaaagtc ctgtcggcct acaataagca     3960

tagagataag ccgatcagag aacaggccga gaacattatc cacttgttca ccctgactaa     4020

cctgggagct ccagccgcct tcaagtactt cgatactact atcgaccgca aaagatacac     4080

gtccaccaag gaagttctgg acgcgaccct gatccaccaa agcatcactg gactctacga     4140

aactaggatc gatctgtcgc agctgggtgg cgatggtggc ggtggatcct acccatacga     4200

cgtgcctgac tacgcctccg gaggtggtgg ccccaagaag aaacggaagg tgtgatagct     4260

agccatcaca tttaaaagca tctcagccta ccatgagaat aagagaaaga aaatgaagat     4320

caatagctta ttcatctctt tttctttttc gttggtgtaa agccaacacc ctgtctaaaa     4380

aacataaatt tctttaatca ttttgcctct tttctctgtg cttcaattaa taaaaaatgg     4440

aaagaacctc gag                                                        4453


<210>  248

<400>  248
000

<210>  249

<400>  249
000

<210>  250

<400>  250
000

<210>  251

<400>  251
000

<210>  252
<211>  4140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 ORF with minimal uridine codons frequently used 
       in humans in general; 12.75% U content

<400>  252
atggacaaga agtacagcat cggcctggac atcggcacca acagcgtggg ctgggccgtg       60

atcaccgacg agtacaaggt gcccagcaag aagttcaagg tgctgggcaa caccgacaga      120

cacagcatca agaagaacct gatcggcgcc ctgctgttcg acagcggcga gaccgccgag      180

gccaccagac tgaagagaac cgccagaaga agatacacca gaagaaagaa cagaatctgc      240

tacctgcagg agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga      300

ctggaggaga gcttcctggt ggaggaggac aagaagcacg agagacaccc catcttcggc      360

aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag      420

aagctggtgg acagcaccga caaggccgac ctgagactga tctacctggc cctggcccac      480

atgatcaagt tcagaggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac      540

gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggagaacccc      600

atcaacgcca gcggcgtgga cgccaaggcc atcctgagcg ccagactgag caagagcaga      660

agactggaga acctgatcgc ccagctgccc ggcgagaaga agaacggcct gttcggcaac      720

ctgatcgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag      780

gacgccaagc tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc      840

cagatcggcg accagtacgc cgacctgttc ctggccgcca agaacctgag cgacgccatc      900

ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgccagc      960

atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaggc cctggtgaga     1020

cagcagctgc ccgagaagta caaggagatc ttcttcgacc agagcaagaa cggctacgcc     1080

ggctacatcg acggcggcgc cagccaggag gagttctaca agttcatcaa gcccatcctg     1140

gagaagatgg acggcaccga ggagctgctg gtgaagctga acagagagga cctgctgaga     1200

aagcagagaa ccttcgacaa cggcagcatc ccccaccaga tccacctggg cgagctgcac     1260

gccatcctga gaagacagga ggacttctac cccttcctga aggacaacag agagaagatc     1320

gagaagatcc tgaccttcag aatcccctac tacgtgggcc ccctggccag aggcaacagc     1380

agattcgcct ggatgaccag aaagagcgag gagaccatca ccccctggaa cttcgaggag     1440

gtggtggaca agggcgccag cgcccagagc ttcatcgaga gaatgaccaa cttcgacaag     1500

aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg     1560

tacaacgagc tgaccaaggt gaagtacgtg accgagggca tgagaaagcc cgccttcctg     1620

agcggcgagc agaagaaggc catcgtggac ctgctgttca agaccaacag aaaggtgacc     1680

gtgaagcagc tgaaggagga ctacttcaag aagatcgagt gcttcgacag cgtggagatc     1740

agcggcgtgg aggacagatt caacgccagc ctgggcacct accacgacct gctgaagatc     1800

atcaaggaca aggacttcct ggacaacgag gagaacgagg acatcctgga ggacatcgtg     1860

ctgaccctga ccctgttcga ggacagagag atgatcgagg agagactgaa gacctacgcc     1920

cacctgttcg acgacaaggt gatgaagcag ctgaagagaa gaagatacac cggctggggc     1980

agactgagca gaaagctgat caacggcatc agagacaagc agagcggcaa gaccatcctg     2040

gacttcctga agagcgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac     2100

agcctgacct tcaaggagga catccagaag gcccaggtga gcggccaggg cgacagcctg     2160

cacgagcaca tcgccaacct ggccggcagc cccgccatca agaagggcat cctgcagacc     2220

gtgaaggtgg tggacgagct ggtgaaggtg atgggcagac acaagcccga gaacatcgtg     2280

atcgagatgg ccagagagaa ccagaccacc cagaagggcc agaagaacag cagagagaga     2340

atgaagagaa tcgaggaggg catcaaggag ctgggcagcc agatcctgaa ggagcacccc     2400

gtggagaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaacggcaga     2460

gacatgtacg tggaccagga gctggacatc aacagactga gcgactacga cgtggaccac     2520

atcgtgcccc agagcttcct gaaggacgac agcatcgaca acaaggtgct gaccagaagc     2580

gacaagaaca gaggcaagag cgacaacgtg cccagcgagg aggtggtgaa gaagatgaag     2640

aactactgga gacagctgct gaacgccaag ctgatcaccc agagaaagtt cgacaacctg     2700

accaaggccg agagaggcgg cctgagcgag ctggacaagg ccggcttcat caagagacag     2760

ctggtggaga ccagacagat caccaagcac gtggcccaga tcctggacag cagaatgaac     2820

accaagtacg acgagaacga caagctgatc agagaggtga aggtgatcac cctgaagagc     2880

aagctggtga gcgacttcag aaaggacttc cagttctaca aggtgagaga gatcaacaac     2940

taccaccacg cccacgacgc ctacctgaac gccgtggtgg gcaccgccct gatcaagaag     3000

taccccaagc tggagagcga gttcgtgtac ggcgactaca aggtgtacga cgtgagaaag     3060

atgatcgcca agagcgagca ggagatcggc aaggccaccg ccaagtactt cttctacagc     3120

aacatcatga acttcttcaa gaccgagatc accctggcca acggcgagat cagaaagaga     3180

cccctgatcg agaccaacgg cgagaccggc gagatcgtgt gggacaaggg cagagacttc     3240

gccaccgtga gaaaggtgct gagcatgccc caggtgaaca tcgtgaagaa gaccgaggtg     3300

cagaccggcg gcttcagcaa ggagagcatc ctgcccaaga gaaacagcga caagctgatc     3360

gccagaaaga aggactggga ccccaagaag tacggcggct tcgacagccc caccgtggcc     3420

tacagcgtgc tggtggtggc caaggtggag aagggcaaga gcaagaagct gaagagcgtg     3480

aaggagctgc tgggcatcac catcatggag agaagcagct tcgagaagaa ccccatcgac     3540

ttcctggagg ccaagggcta caaggaggtg aagaaggacc tgatcatcaa gctgcccaag     3600

tacagcctgt tcgagctgga gaacggcaga aagagaatgc tggccagcgc cggcgagctg     3660

cagaagggca acgagctggc cctgcccagc aagtacgtga acttcctgta cctggccagc     3720

cactacgaga agctgaaggg cagccccgag gacaacgagc agaagcagct gttcgtggag     3780

cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttcag caagagagtg     3840

atcctggccg acgccaacct ggacaaggtg ctgagcgcct acaacaagca cagagacaag     3900

cccatcagag agcaggccga gaacatcatc cacctgttca ccctgaccaa cctgggcgcc     3960

cccgccgcct tcaagtactt cgacaccacc atcgacagaa agagatacac cagcaccaag     4020

gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gaccagaatc     4080

gacctgagcc agctgggcgg cgacggcggc ggcagcccca agaagaagag aaaggtgtga     4140


<210>  253
<211>  4411
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript with 5 UTR of HSD, ORF corresponding 
       to SEQ ID NO: 252, Kozak sequence, and 3 UTR of ALB

<400>  253
gggtcccgca gtcggcgtcc agcggctctg cttgttcgtg tgtgtgtcgt tgcaggcctt       60

attcggatcc gccaccatgg acaagaagta cagcatcggc ctggacatcg gcaccaacag      120

cgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaagt tcaaggtgct      180

gggcaacacc gacagacaca gcatcaagaa gaacctgatc ggcgccctgc tgttcgacag      240

cggcgagacc gccgaggcca ccagactgaa gagaaccgcc agaagaagat acaccagaag      300

aaagaacaga atctgctacc tgcaggagat cttcagcaac gagatggcca aggtggacga      360

cagcttcttc cacagactgg aggagagctt cctggtggag gaggacaaga agcacgagag      420

acaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat      480

ctaccacctg agaaagaagc tggtggacag caccgacaag gccgacctga gactgatcta      540

cctggccctg gcccacatga tcaagttcag aggccacttc ctgatcgagg gcgacctgaa      600

ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct      660

gttcgaggag aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgagcgccag      720

actgagcaag agcagaagac tggagaacct gatcgcccag ctgcccggcg agaagaagaa      780

cggcctgttc ggcaacctga tcgccctgag cctgggcctg acccccaact tcaagagcaa      840

cttcgacctg gccgaggacg ccaagctgca gctgagcaag gacacctacg acgacgacct      900

ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgttcctgg ccgccaagaa      960

cctgagcgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc     1020

ccccctgagc gccagcatga tcaagagata cgacgagcac caccaggacc tgaccctgct     1080

gaaggccctg gtgagacagc agctgcccga gaagtacaag gagatcttct tcgaccagag     1140

caagaacggc tacgccggct acatcgacgg cggcgccagc caggaggagt tctacaagtt     1200

catcaagccc atcctggaga agatggacgg caccgaggag ctgctggtga agctgaacag     1260

agaggacctg ctgagaaagc agagaacctt cgacaacggc agcatccccc accagatcca     1320

cctgggcgag ctgcacgcca tcctgagaag acaggaggac ttctacccct tcctgaagga     1380

caacagagag aagatcgaga agatcctgac cttcagaatc ccctactacg tgggccccct     1440

ggccagaggc aacagcagat tcgcctggat gaccagaaag agcgaggaga ccatcacccc     1500

ctggaacttc gaggaggtgg tggacaaggg cgccagcgcc cagagcttca tcgagagaat     1560

gaccaacttc gacaagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta     1620

cgagtacttc accgtgtaca acgagctgac caaggtgaag tacgtgaccg agggcatgag     1680

aaagcccgcc ttcctgagcg gcgagcagaa gaaggccatc gtggacctgc tgttcaagac     1740

caacagaaag gtgaccgtga agcagctgaa ggaggactac ttcaagaaga tcgagtgctt     1800

cgacagcgtg gagatcagcg gcgtggagga cagattcaac gccagcctgg gcacctacca     1860

cgacctgctg aagatcatca aggacaagga cttcctggac aacgaggaga acgaggacat     1920

cctggaggac atcgtgctga ccctgaccct gttcgaggac agagagatga tcgaggagag     1980

actgaagacc tacgcccacc tgttcgacga caaggtgatg aagcagctga agagaagaag     2040

atacaccggc tggggcagac tgagcagaaa gctgatcaac ggcatcagag acaagcagag     2100

cggcaagacc atcctggact tcctgaagag cgacggcttc gccaacagaa acttcatgca     2160

gctgatccac gacgacagcc tgaccttcaa ggaggacatc cagaaggccc aggtgagcgg     2220

ccagggcgac agcctgcacg agcacatcgc caacctggcc ggcagccccg ccatcaagaa     2280

gggcatcctg cagaccgtga aggtggtgga cgagctggtg aaggtgatgg gcagacacaa     2340

gcccgagaac atcgtgatcg agatggccag agagaaccag accacccaga agggccagaa     2400

gaacagcaga gagagaatga agagaatcga ggagggcatc aaggagctgg gcagccagat     2460

cctgaaggag caccccgtgg agaacaccca gctgcagaac gagaagctgt acctgtacta     2520

cctgcagaac ggcagagaca tgtacgtgga ccaggagctg gacatcaaca gactgagcga     2580

ctacgacgtg gaccacatcg tgccccagag cttcctgaag gacgacagca tcgacaacaa     2640

ggtgctgacc agaagcgaca agaacagagg caagagcgac aacgtgccca gcgaggaggt     2700

ggtgaagaag atgaagaact actggagaca gctgctgaac gccaagctga tcacccagag     2760

aaagttcgac aacctgacca aggccgagag aggcggcctg agcgagctgg acaaggccgg     2820

cttcatcaag agacagctgg tggagaccag acagatcacc aagcacgtgg cccagatcct     2880

ggacagcaga atgaacacca agtacgacga gaacgacaag ctgatcagag aggtgaaggt     2940

gatcaccctg aagagcaagc tggtgagcga cttcagaaag gacttccagt tctacaaggt     3000

gagagagatc aacaactacc accacgccca cgacgcctac ctgaacgccg tggtgggcac     3060

cgccctgatc aagaagtacc ccaagctgga gagcgagttc gtgtacggcg actacaaggt     3120

gtacgacgtg agaaagatga tcgccaagag cgagcaggag atcggcaagg ccaccgccaa     3180

gtacttcttc tacagcaaca tcatgaactt cttcaagacc gagatcaccc tggccaacgg     3240

cgagatcaga aagagacccc tgatcgagac caacggcgag accggcgaga tcgtgtggga     3300

caagggcaga gacttcgcca ccgtgagaaa ggtgctgagc atgccccagg tgaacatcgt     3360

gaagaagacc gaggtgcaga ccggcggctt cagcaaggag agcatcctgc ccaagagaaa     3420

cagcgacaag ctgatcgcca gaaagaagga ctgggacccc aagaagtacg gcggcttcga     3480

cagccccacc gtggcctaca gcgtgctggt ggtggccaag gtggagaagg gcaagagcaa     3540

gaagctgaag agcgtgaagg agctgctggg catcaccatc atggagagaa gcagcttcga     3600

gaagaacccc atcgacttcc tggaggccaa gggctacaag gaggtgaaga aggacctgat     3660

catcaagctg cccaagtaca gcctgttcga gctggagaac ggcagaaaga gaatgctggc     3720

cagcgccggc gagctgcaga agggcaacga gctggccctg cccagcaagt acgtgaactt     3780

cctgtacctg gccagccact acgagaagct gaagggcagc cccgaggaca acgagcagaa     3840

gcagctgttc gtggagcagc acaagcacta cctggacgag atcatcgagc agatcagcga     3900

gttcagcaag agagtgatcc tggccgacgc caacctggac aaggtgctga gcgcctacaa     3960

caagcacaga gacaagccca tcagagagca ggccgagaac atcatccacc tgttcaccct     4020

gaccaacctg ggcgcccccg ccgccttcaa gtacttcgac accaccatcg acagaaagag     4080

atacaccagc accaaggagg tgctggacgc caccctgatc caccagagca tcaccggcct     4140

gtacgagacc agaatcgacc tgagccagct gggcggcgac ggcggcggca gccccaagaa     4200

gaagagaaag gtgtgactag ccatcacatt taaaagcatc tcagcctacc atgagaataa     4260

gagaaagaaa atgaagatca atagcttatt catctctttt tctttttcgt tggtgtaaag     4320

ccaacaccct gtctaaaaaa cataaatttc tttaatcatt ttgcctcttt tctctgtgct     4380

tcaattaata aaaaatggaa agaacctcga g                                    4411


<210>  254

<400>  254
000

<210>  255

<400>  255
000

<210>  256
<211>  4411
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript with AGG as first three nucleotides 
       for use with CleanCapTM, 5 UTR of HSD, ORF corresponding to SEQ 
       ID NO: 204, Kozak sequence, and 3 UTR of ALB

<400>  256
aggtcccgca gtcggcgtcc agcggctctg cttgttcgtg tgtgtgtcgt tgcaggcctt       60

attcggatcc gccaccatgg acaagaagta cagcatcgga ctggacatcg gaacaaacag      120

cgtcggatgg gcagtcatca cagacgaata caaggtcccg agcaagaagt tcaaggtcct      180

gggaaacaca gacagacaca gcatcaagaa gaacctgatc ggagcactgc tgttcgacag      240

cggagaaaca gcagaagcaa caagactgaa gagaacagca agaagaagat acacaagaag      300

aaagaacaga atctgctacc tgcaggaaat cttcagcaac gaaatggcaa aggtcgacga      360

cagcttcttc cacagactgg aagaaagctt cctggtcgaa gaagacaaga agcacgaaag      420

acacccgatc ttcggaaaca tcgtcgacga agtcgcatac cacgaaaagt acccgacaat      480

ctaccacctg agaaagaagc tggtcgacag cacagacaag gcagacctga gactgatcta      540

cctggcactg gcacacatga tcaagttcag aggacacttc ctgatcgaag gagacctgaa      600

cccggacaac agcgacgtcg acaagctgtt catccagctg gtccagacat acaaccagct      660

gttcgaagaa aacccgatca acgcaagcgg agtcgacgca aaggcaatcc tgagcgcaag      720

actgagcaag agcagaagac tggaaaacct gatcgcacag ctgccgggag aaaagaagaa      780

cggactgttc ggaaacctga tcgcactgag cctgggactg acaccgaact tcaagagcaa      840

cttcgacctg gcagaagacg caaagctgca gctgagcaag gacacatacg acgacgacct      900

ggacaacctg ctggcacaga tcggagacca gtacgcagac ctgttcctgg cagcaaagaa      960

cctgagcgac gcaatcctgc tgagcgacat cctgagagtc aacacagaaa tcacaaaggc     1020

accgctgagc gcaagcatga tcaagagata cgacgaacac caccaggacc tgacactgct     1080

gaaggcactg gtcagacagc agctgccgga aaagtacaag gaaatcttct tcgaccagag     1140

caagaacgga tacgcaggat acatcgacgg aggagcaagc caggaagaat tctacaagtt     1200

catcaagccg atcctggaaa agatggacgg aacagaagaa ctgctggtca agctgaacag     1260

agaagacctg ctgagaaagc agagaacatt cgacaacgga agcatcccgc accagatcca     1320

cctgggagaa ctgcacgcaa tcctgagaag acaggaagac ttctacccgt tcctgaagga     1380

caacagagaa aagatcgaaa agatcctgac attcagaatc ccgtactacg tcggaccgct     1440

ggcaagagga aacagcagat tcgcatggat gacaagaaag agcgaagaaa caatcacacc     1500

gtggaacttc gaagaagtcg tcgacaaggg agcaagcgca cagagcttca tcgaaagaat     1560

gacaaacttc gacaagaacc tgccgaacga aaaggtcctg ccgaagcaca gcctgctgta     1620

cgaatacttc acagtctaca acgaactgac aaaggtcaag tacgtcacag aaggaatgag     1680

aaagccggca ttcctgagcg gagaacagaa gaaggcaatc gtcgacctgc tgttcaagac     1740

aaacagaaag gtcacagtca agcagctgaa ggaagactac ttcaagaaga tcgaatgctt     1800

cgacagcgtc gaaatcagcg gagtcgaaga cagattcaac gcaagcctgg gaacatacca     1860

cgacctgctg aagatcatca aggacaagga cttcctggac aacgaagaaa acgaagacat     1920

cctggaagac atcgtcctga cactgacact gttcgaagac agagaaatga tcgaagaaag     1980

actgaagaca tacgcacacc tgttcgacga caaggtcatg aagcagctga agagaagaag     2040

atacacagga tggggaagac tgagcagaaa gctgatcaac ggaatcagag acaagcagag     2100

cggaaagaca atcctggact tcctgaagag cgacggattc gcaaacagaa acttcatgca     2160

gctgatccac gacgacagcc tgacattcaa ggaagacatc cagaaggcac aggtcagcgg     2220

acagggagac agcctgcacg aacacatcgc aaacctggca ggaagcccgg caatcaagaa     2280

gggaatcctg cagacagtca aggtcgtcga cgaactggtc aaggtcatgg gaagacacaa     2340

gccggaaaac atcgtcatcg aaatggcaag agaaaaccag acaacacaga agggacagaa     2400

gaacagcaga gaaagaatga agagaatcga agaaggaatc aaggaactgg gaagccagat     2460

cctgaaggaa cacccggtcg aaaacacaca gctgcagaac gaaaagctgt acctgtacta     2520

cctgcagaac ggaagagaca tgtacgtcga ccaggaactg gacatcaaca gactgagcga     2580

ctacgacgtc gaccacatcg tcccgcagag cttcctgaag gacgacagca tcgacaacaa     2640

ggtcctgaca agaagcgaca agaacagagg aaagagcgac aacgtcccga gcgaagaagt     2700

cgtcaagaag atgaagaact actggagaca gctgctgaac gcaaagctga tcacacagag     2760

aaagttcgac aacctgacaa aggcagagag aggaggactg agcgaactgg acaaggcagg     2820

attcatcaag agacagctgg tcgaaacaag acagatcaca aagcacgtcg cacagatcct     2880

ggacagcaga atgaacacaa agtacgacga aaacgacaag ctgatcagag aagtcaaggt     2940

catcacactg aagagcaagc tggtcagcga cttcagaaag gacttccagt tctacaaggt     3000

cagagaaatc aacaactacc accacgcaca cgacgcatac ctgaacgcag tcgtcggaac     3060

agcactgatc aagaagtacc cgaagctgga aagcgaattc gtctacggag actacaaggt     3120

ctacgacgtc agaaagatga tcgcaaagag cgaacaggaa atcggaaagg caacagcaaa     3180

gtacttcttc tacagcaaca tcatgaactt cttcaagaca gaaatcacac tggcaaacgg     3240

agaaatcaga aagagaccgc tgatcgaaac aaacggagaa acaggagaaa tcgtctggga     3300

caagggaaga gacttcgcaa cagtcagaaa ggtcctgagc atgccgcagg tcaacatcgt     3360

caagaagaca gaagtccaga caggaggatt cagcaaggaa agcatcctgc cgaagagaaa     3420

cagcgacaag ctgatcgcaa gaaagaagga ctgggacccg aagaagtacg gaggattcga     3480

cagcccgaca gtcgcataca gcgtcctggt cgtcgcaaag gtcgaaaagg gaaagagcaa     3540

gaagctgaag agcgtcaagg aactgctggg aatcacaatc atggaaagaa gcagcttcga     3600

aaagaacccg atcgacttcc tggaagcaaa gggatacaag gaagtcaaga aggacctgat     3660

catcaagctg ccgaagtaca gcctgttcga actggaaaac ggaagaaaga gaatgctggc     3720

aagcgcagga gaactgcaga agggaaacga actggcactg ccgagcaagt acgtcaactt     3780

cctgtacctg gcaagccact acgaaaagct gaagggaagc ccggaagaca acgaacagaa     3840

gcagctgttc gtcgaacagc acaagcacta cctggacgaa atcatcgaac agatcagcga     3900

attcagcaag agagtcatcc tggcagacgc aaacctggac aaggtcctga gcgcatacaa     3960

caagcacaga gacaagccga tcagagaaca ggcagaaaac atcatccacc tgttcacact     4020

gacaaacctg ggagcaccgg cagcattcaa gtacttcgac acaacaatcg acagaaagag     4080

atacacaagc acaaaggaag tcctggacgc aacactgatc caccagagca tcacaggact     4140

gtacgaaaca agaatcgacc tgagccagct gggaggagac ggaggaggaa gcccgaagaa     4200

gaagagaaag gtctagctag ccatcacatt taaaagcatc tcagcctacc atgagaataa     4260

gagaaagaaa atgaagatca atagcttatt catctctttt tctttttcgt tggtgtaaag     4320

ccaacaccct gtctaaaaaa cataaatttc tttaatcatt ttgcctcttt tctctgtgct     4380

tcaattaata aaaaatggaa agaacctcga g                                    4411


<210>  257
<211>  4481
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript with 5 UTR from CMV, ORF corresponding
       to SEQ ID NO: 204, Kozak sequence, and 3 UTR of ALB

<400>  257
gggcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac       60

cgatccagcc tccgcggccg ggaacggtgc attggaacgc ggattccccg tgccaagagt      120

gactcaccgt ccttgacacg gccaccatgg acaagaagta cagcatcgga ctggacatcg      180

gaacaaacag cgtcggatgg gcagtcatca cagacgaata caaggtcccg agcaagaagt      240

tcaaggtcct gggaaacaca gacagacaca gcatcaagaa gaacctgatc ggagcactgc      300

tgttcgacag cggagaaaca gcagaagcaa caagactgaa gagaacagca agaagaagat      360

acacaagaag aaagaacaga atctgctacc tgcaggaaat cttcagcaac gaaatggcaa      420

aggtcgacga cagcttcttc cacagactgg aagaaagctt cctggtcgaa gaagacaaga      480

agcacgaaag acacccgatc ttcggaaaca tcgtcgacga agtcgcatac cacgaaaagt      540

acccgacaat ctaccacctg agaaagaagc tggtcgacag cacagacaag gcagacctga      600

gactgatcta cctggcactg gcacacatga tcaagttcag aggacacttc ctgatcgaag      660

gagacctgaa cccggacaac agcgacgtcg acaagctgtt catccagctg gtccagacat      720

acaaccagct gttcgaagaa aacccgatca acgcaagcgg agtcgacgca aaggcaatcc      780

tgagcgcaag actgagcaag agcagaagac tggaaaacct gatcgcacag ctgccgggag      840

aaaagaagaa cggactgttc ggaaacctga tcgcactgag cctgggactg acaccgaact      900

tcaagagcaa cttcgacctg gcagaagacg caaagctgca gctgagcaag gacacatacg      960

acgacgacct ggacaacctg ctggcacaga tcggagacca gtacgcagac ctgttcctgg     1020

cagcaaagaa cctgagcgac gcaatcctgc tgagcgacat cctgagagtc aacacagaaa     1080

tcacaaaggc accgctgagc gcaagcatga tcaagagata cgacgaacac caccaggacc     1140

tgacactgct gaaggcactg gtcagacagc agctgccgga aaagtacaag gaaatcttct     1200

tcgaccagag caagaacgga tacgcaggat acatcgacgg aggagcaagc caggaagaat     1260

tctacaagtt catcaagccg atcctggaaa agatggacgg aacagaagaa ctgctggtca     1320

agctgaacag agaagacctg ctgagaaagc agagaacatt cgacaacgga agcatcccgc     1380

accagatcca cctgggagaa ctgcacgcaa tcctgagaag acaggaagac ttctacccgt     1440

tcctgaagga caacagagaa aagatcgaaa agatcctgac attcagaatc ccgtactacg     1500

tcggaccgct ggcaagagga aacagcagat tcgcatggat gacaagaaag agcgaagaaa     1560

caatcacacc gtggaacttc gaagaagtcg tcgacaaggg agcaagcgca cagagcttca     1620

tcgaaagaat gacaaacttc gacaagaacc tgccgaacga aaaggtcctg ccgaagcaca     1680

gcctgctgta cgaatacttc acagtctaca acgaactgac aaaggtcaag tacgtcacag     1740

aaggaatgag aaagccggca ttcctgagcg gagaacagaa gaaggcaatc gtcgacctgc     1800

tgttcaagac aaacagaaag gtcacagtca agcagctgaa ggaagactac ttcaagaaga     1860

tcgaatgctt cgacagcgtc gaaatcagcg gagtcgaaga cagattcaac gcaagcctgg     1920

gaacatacca cgacctgctg aagatcatca aggacaagga cttcctggac aacgaagaaa     1980

acgaagacat cctggaagac atcgtcctga cactgacact gttcgaagac agagaaatga     2040

tcgaagaaag actgaagaca tacgcacacc tgttcgacga caaggtcatg aagcagctga     2100

agagaagaag atacacagga tggggaagac tgagcagaaa gctgatcaac ggaatcagag     2160

acaagcagag cggaaagaca atcctggact tcctgaagag cgacggattc gcaaacagaa     2220

acttcatgca gctgatccac gacgacagcc tgacattcaa ggaagacatc cagaaggcac     2280

aggtcagcgg acagggagac agcctgcacg aacacatcgc aaacctggca ggaagcccgg     2340

caatcaagaa gggaatcctg cagacagtca aggtcgtcga cgaactggtc aaggtcatgg     2400

gaagacacaa gccggaaaac atcgtcatcg aaatggcaag agaaaaccag acaacacaga     2460

agggacagaa gaacagcaga gaaagaatga agagaatcga agaaggaatc aaggaactgg     2520

gaagccagat cctgaaggaa cacccggtcg aaaacacaca gctgcagaac gaaaagctgt     2580

acctgtacta cctgcagaac ggaagagaca tgtacgtcga ccaggaactg gacatcaaca     2640

gactgagcga ctacgacgtc gaccacatcg tcccgcagag cttcctgaag gacgacagca     2700

tcgacaacaa ggtcctgaca agaagcgaca agaacagagg aaagagcgac aacgtcccga     2760

gcgaagaagt cgtcaagaag atgaagaact actggagaca gctgctgaac gcaaagctga     2820

tcacacagag aaagttcgac aacctgacaa aggcagagag aggaggactg agcgaactgg     2880

acaaggcagg attcatcaag agacagctgg tcgaaacaag acagatcaca aagcacgtcg     2940

cacagatcct ggacagcaga atgaacacaa agtacgacga aaacgacaag ctgatcagag     3000

aagtcaaggt catcacactg aagagcaagc tggtcagcga cttcagaaag gacttccagt     3060

tctacaaggt cagagaaatc aacaactacc accacgcaca cgacgcatac ctgaacgcag     3120

tcgtcggaac agcactgatc aagaagtacc cgaagctgga aagcgaattc gtctacggag     3180

actacaaggt ctacgacgtc agaaagatga tcgcaaagag cgaacaggaa atcggaaagg     3240

caacagcaaa gtacttcttc tacagcaaca tcatgaactt cttcaagaca gaaatcacac     3300

tggcaaacgg agaaatcaga aagagaccgc tgatcgaaac aaacggagaa acaggagaaa     3360

tcgtctggga caagggaaga gacttcgcaa cagtcagaaa ggtcctgagc atgccgcagg     3420

tcaacatcgt caagaagaca gaagtccaga caggaggatt cagcaaggaa agcatcctgc     3480

cgaagagaaa cagcgacaag ctgatcgcaa gaaagaagga ctgggacccg aagaagtacg     3540

gaggattcga cagcccgaca gtcgcataca gcgtcctggt cgtcgcaaag gtcgaaaagg     3600

gaaagagcaa gaagctgaag agcgtcaagg aactgctggg aatcacaatc atggaaagaa     3660

gcagcttcga aaagaacccg atcgacttcc tggaagcaaa gggatacaag gaagtcaaga     3720

aggacctgat catcaagctg ccgaagtaca gcctgttcga actggaaaac ggaagaaaga     3780

gaatgctggc aagcgcagga gaactgcaga agggaaacga actggcactg ccgagcaagt     3840

acgtcaactt cctgtacctg gcaagccact acgaaaagct gaagggaagc ccggaagaca     3900

acgaacagaa gcagctgttc gtcgaacagc acaagcacta cctggacgaa atcatcgaac     3960

agatcagcga attcagcaag agagtcatcc tggcagacgc aaacctggac aaggtcctga     4020

gcgcatacaa caagcacaga gacaagccga tcagagaaca ggcagaaaac atcatccacc     4080

tgttcacact gacaaacctg ggagcaccgg cagcattcaa gtacttcgac acaacaatcg     4140

acagaaagag atacacaagc acaaaggaag tcctggacgc aacactgatc caccagagca     4200

tcacaggact gtacgaaaca agaatcgacc tgagccagct gggaggagac ggaggaggaa     4260

gcccgaagaa gaagagaaag gtctagctag ccatcacatt taaaagcatc tcagcctacc     4320

atgagaataa gagaaagaaa atgaagatca atagcttatt catctctttt tctttttcgt     4380

tggtgtaaag ccaacaccct gtctaaaaaa cataaatttc tttaatcatt ttgcctcttt     4440

tctctgtgct tcaattaata aaaaatggaa agaacctcga g                         4481


<210>  258
<211>  4348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript with 5 UTR from HBB, ORF corresponding
       to SEQ ID NO: 204, Kozak sequence, and 3 UTR of HBB

<400>  258
gggacatttg cttctgacac aactgtgttc actagcaacc tcaaacagac accggatctg       60

ccaccatgga caagaagtac agcatcggac tggacatcgg aacaaacagc gtcggatggg      120

cagtcatcac agacgaatac aaggtcccga gcaagaagtt caaggtcctg ggaaacacag      180

acagacacag catcaagaag aacctgatcg gagcactgct gttcgacagc ggagaaacag      240

cagaagcaac aagactgaag agaacagcaa gaagaagata cacaagaaga aagaacagaa      300

tctgctacct gcaggaaatc ttcagcaacg aaatggcaaa ggtcgacgac agcttcttcc      360

acagactgga agaaagcttc ctggtcgaag aagacaagaa gcacgaaaga cacccgatct      420

tcggaaacat cgtcgacgaa gtcgcatacc acgaaaagta cccgacaatc taccacctga      480

gaaagaagct ggtcgacagc acagacaagg cagacctgag actgatctac ctggcactgg      540

cacacatgat caagttcaga ggacacttcc tgatcgaagg agacctgaac ccggacaaca      600

gcgacgtcga caagctgttc atccagctgg tccagacata caaccagctg ttcgaagaaa      660

acccgatcaa cgcaagcgga gtcgacgcaa aggcaatcct gagcgcaaga ctgagcaaga      720

gcagaagact ggaaaacctg atcgcacagc tgccgggaga aaagaagaac ggactgttcg      780

gaaacctgat cgcactgagc ctgggactga caccgaactt caagagcaac ttcgacctgg      840

cagaagacgc aaagctgcag ctgagcaagg acacatacga cgacgacctg gacaacctgc      900

tggcacagat cggagaccag tacgcagacc tgttcctggc agcaaagaac ctgagcgacg      960

caatcctgct gagcgacatc ctgagagtca acacagaaat cacaaaggca ccgctgagcg     1020

caagcatgat caagagatac gacgaacacc accaggacct gacactgctg aaggcactgg     1080

tcagacagca gctgccggaa aagtacaagg aaatcttctt cgaccagagc aagaacggat     1140

acgcaggata catcgacgga ggagcaagcc aggaagaatt ctacaagttc atcaagccga     1200

tcctggaaaa gatggacgga acagaagaac tgctggtcaa gctgaacaga gaagacctgc     1260

tgagaaagca gagaacattc gacaacggaa gcatcccgca ccagatccac ctgggagaac     1320

tgcacgcaat cctgagaaga caggaagact tctacccgtt cctgaaggac aacagagaaa     1380

agatcgaaaa gatcctgaca ttcagaatcc cgtactacgt cggaccgctg gcaagaggaa     1440

acagcagatt cgcatggatg acaagaaaga gcgaagaaac aatcacaccg tggaacttcg     1500

aagaagtcgt cgacaaggga gcaagcgcac agagcttcat cgaaagaatg acaaacttcg     1560

acaagaacct gccgaacgaa aaggtcctgc cgaagcacag cctgctgtac gaatacttca     1620

cagtctacaa cgaactgaca aaggtcaagt acgtcacaga aggaatgaga aagccggcat     1680

tcctgagcgg agaacagaag aaggcaatcg tcgacctgct gttcaagaca aacagaaagg     1740

tcacagtcaa gcagctgaag gaagactact tcaagaagat cgaatgcttc gacagcgtcg     1800

aaatcagcgg agtcgaagac agattcaacg caagcctggg aacataccac gacctgctga     1860

agatcatcaa ggacaaggac ttcctggaca acgaagaaaa cgaagacatc ctggaagaca     1920

tcgtcctgac actgacactg ttcgaagaca gagaaatgat cgaagaaaga ctgaagacat     1980

acgcacacct gttcgacgac aaggtcatga agcagctgaa gagaagaaga tacacaggat     2040

ggggaagact gagcagaaag ctgatcaacg gaatcagaga caagcagagc ggaaagacaa     2100

tcctggactt cctgaagagc gacggattcg caaacagaaa cttcatgcag ctgatccacg     2160

acgacagcct gacattcaag gaagacatcc agaaggcaca ggtcagcgga cagggagaca     2220

gcctgcacga acacatcgca aacctggcag gaagcccggc aatcaagaag ggaatcctgc     2280

agacagtcaa ggtcgtcgac gaactggtca aggtcatggg aagacacaag ccggaaaaca     2340

tcgtcatcga aatggcaaga gaaaaccaga caacacagaa gggacagaag aacagcagag     2400

aaagaatgaa gagaatcgaa gaaggaatca aggaactggg aagccagatc ctgaaggaac     2460

acccggtcga aaacacacag ctgcagaacg aaaagctgta cctgtactac ctgcagaacg     2520

gaagagacat gtacgtcgac caggaactgg acatcaacag actgagcgac tacgacgtcg     2580

accacatcgt cccgcagagc ttcctgaagg acgacagcat cgacaacaag gtcctgacaa     2640

gaagcgacaa gaacagagga aagagcgaca acgtcccgag cgaagaagtc gtcaagaaga     2700

tgaagaacta ctggagacag ctgctgaacg caaagctgat cacacagaga aagttcgaca     2760

acctgacaaa ggcagagaga ggaggactga gcgaactgga caaggcagga ttcatcaaga     2820

gacagctggt cgaaacaaga cagatcacaa agcacgtcgc acagatcctg gacagcagaa     2880

tgaacacaaa gtacgacgaa aacgacaagc tgatcagaga agtcaaggtc atcacactga     2940

agagcaagct ggtcagcgac ttcagaaagg acttccagtt ctacaaggtc agagaaatca     3000

acaactacca ccacgcacac gacgcatacc tgaacgcagt cgtcggaaca gcactgatca     3060

agaagtaccc gaagctggaa agcgaattcg tctacggaga ctacaaggtc tacgacgtca     3120

gaaagatgat cgcaaagagc gaacaggaaa tcggaaaggc aacagcaaag tacttcttct     3180

acagcaacat catgaacttc ttcaagacag aaatcacact ggcaaacgga gaaatcagaa     3240

agagaccgct gatcgaaaca aacggagaaa caggagaaat cgtctgggac aagggaagag     3300

acttcgcaac agtcagaaag gtcctgagca tgccgcaggt caacatcgtc aagaagacag     3360

aagtccagac aggaggattc agcaaggaaa gcatcctgcc gaagagaaac agcgacaagc     3420

tgatcgcaag aaagaaggac tgggacccga agaagtacgg aggattcgac agcccgacag     3480

tcgcatacag cgtcctggtc gtcgcaaagg tcgaaaaggg aaagagcaag aagctgaaga     3540

gcgtcaagga actgctggga atcacaatca tggaaagaag cagcttcgaa aagaacccga     3600

tcgacttcct ggaagcaaag ggatacaagg aagtcaagaa ggacctgatc atcaagctgc     3660

cgaagtacag cctgttcgaa ctggaaaacg gaagaaagag aatgctggca agcgcaggag     3720

aactgcagaa gggaaacgaa ctggcactgc cgagcaagta cgtcaacttc ctgtacctgg     3780

caagccacta cgaaaagctg aagggaagcc cggaagacaa cgaacagaag cagctgttcg     3840

tcgaacagca caagcactac ctggacgaaa tcatcgaaca gatcagcgaa ttcagcaaga     3900

gagtcatcct ggcagacgca aacctggaca aggtcctgag cgcatacaac aagcacagag     3960

acaagccgat cagagaacag gcagaaaaca tcatccacct gttcacactg acaaacctgg     4020

gagcaccggc agcattcaag tacttcgaca caacaatcga cagaaagaga tacacaagca     4080

caaaggaagt cctggacgca acactgatcc accagagcat cacaggactg tacgaaacaa     4140

gaatcgacct gagccagctg ggaggagacg gaggaggaag cccgaagaag aagagaaagg     4200

tctagctagc gctcgctttc ttgctgtcca atttctatta aaggttcctt tgttccctaa     4260

gtccaactac taaactgggg gatattatga agggccttga gcatctggat tctgcctaat     4320

aaaaaacatt tattttcatt gcctcgag                                        4348


<210>  259
<211>  4325
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript with 5 UTR from XBG, ORF corresponding
       to SEQ ID NO: 204, Kozak sequence, and 3 UTR of XBG

<400>  259
gggaagctca gaataaacgc tcaactttgg ccggatctgc caccatggac aagaagtaca       60

gcatcggact ggacatcgga acaaacagcg tcggatgggc agtcatcaca gacgaataca      120

aggtcccgag caagaagttc aaggtcctgg gaaacacaga cagacacagc atcaagaaga      180

acctgatcgg agcactgctg ttcgacagcg gagaaacagc agaagcaaca agactgaaga      240

gaacagcaag aagaagatac acaagaagaa agaacagaat ctgctacctg caggaaatct      300

tcagcaacga aatggcaaag gtcgacgaca gcttcttcca cagactggaa gaaagcttcc      360

tggtcgaaga agacaagaag cacgaaagac acccgatctt cggaaacatc gtcgacgaag      420

tcgcatacca cgaaaagtac ccgacaatct accacctgag aaagaagctg gtcgacagca      480

cagacaaggc agacctgaga ctgatctacc tggcactggc acacatgatc aagttcagag      540

gacacttcct gatcgaagga gacctgaacc cggacaacag cgacgtcgac aagctgttca      600

tccagctggt ccagacatac aaccagctgt tcgaagaaaa cccgatcaac gcaagcggag      660

tcgacgcaaa ggcaatcctg agcgcaagac tgagcaagag cagaagactg gaaaacctga      720

tcgcacagct gccgggagaa aagaagaacg gactgttcgg aaacctgatc gcactgagcc      780

tgggactgac accgaacttc aagagcaact tcgacctggc agaagacgca aagctgcagc      840

tgagcaagga cacatacgac gacgacctgg acaacctgct ggcacagatc ggagaccagt      900

acgcagacct gttcctggca gcaaagaacc tgagcgacgc aatcctgctg agcgacatcc      960

tgagagtcaa cacagaaatc acaaaggcac cgctgagcgc aagcatgatc aagagatacg     1020

acgaacacca ccaggacctg acactgctga aggcactggt cagacagcag ctgccggaaa     1080

agtacaagga aatcttcttc gaccagagca agaacggata cgcaggatac atcgacggag     1140

gagcaagcca ggaagaattc tacaagttca tcaagccgat cctggaaaag atggacggaa     1200

cagaagaact gctggtcaag ctgaacagag aagacctgct gagaaagcag agaacattcg     1260

acaacggaag catcccgcac cagatccacc tgggagaact gcacgcaatc ctgagaagac     1320

aggaagactt ctacccgttc ctgaaggaca acagagaaaa gatcgaaaag atcctgacat     1380

tcagaatccc gtactacgtc ggaccgctgg caagaggaaa cagcagattc gcatggatga     1440

caagaaagag cgaagaaaca atcacaccgt ggaacttcga agaagtcgtc gacaagggag     1500

caagcgcaca gagcttcatc gaaagaatga caaacttcga caagaacctg ccgaacgaaa     1560

aggtcctgcc gaagcacagc ctgctgtacg aatacttcac agtctacaac gaactgacaa     1620

aggtcaagta cgtcacagaa ggaatgagaa agccggcatt cctgagcgga gaacagaaga     1680

aggcaatcgt cgacctgctg ttcaagacaa acagaaaggt cacagtcaag cagctgaagg     1740

aagactactt caagaagatc gaatgcttcg acagcgtcga aatcagcgga gtcgaagaca     1800

gattcaacgc aagcctggga acataccacg acctgctgaa gatcatcaag gacaaggact     1860

tcctggacaa cgaagaaaac gaagacatcc tggaagacat cgtcctgaca ctgacactgt     1920

tcgaagacag agaaatgatc gaagaaagac tgaagacata cgcacacctg ttcgacgaca     1980

aggtcatgaa gcagctgaag agaagaagat acacaggatg gggaagactg agcagaaagc     2040

tgatcaacgg aatcagagac aagcagagcg gaaagacaat cctggacttc ctgaagagcg     2100

acggattcgc aaacagaaac ttcatgcagc tgatccacga cgacagcctg acattcaagg     2160

aagacatcca gaaggcacag gtcagcggac agggagacag cctgcacgaa cacatcgcaa     2220

acctggcagg aagcccggca atcaagaagg gaatcctgca gacagtcaag gtcgtcgacg     2280

aactggtcaa ggtcatggga agacacaagc cggaaaacat cgtcatcgaa atggcaagag     2340

aaaaccagac aacacagaag ggacagaaga acagcagaga aagaatgaag agaatcgaag     2400

aaggaatcaa ggaactggga agccagatcc tgaaggaaca cccggtcgaa aacacacagc     2460

tgcagaacga aaagctgtac ctgtactacc tgcagaacgg aagagacatg tacgtcgacc     2520

aggaactgga catcaacaga ctgagcgact acgacgtcga ccacatcgtc ccgcagagct     2580

tcctgaagga cgacagcatc gacaacaagg tcctgacaag aagcgacaag aacagaggaa     2640

agagcgacaa cgtcccgagc gaagaagtcg tcaagaagat gaagaactac tggagacagc     2700

tgctgaacgc aaagctgatc acacagagaa agttcgacaa cctgacaaag gcagagagag     2760

gaggactgag cgaactggac aaggcaggat tcatcaagag acagctggtc gaaacaagac     2820

agatcacaaa gcacgtcgca cagatcctgg acagcagaat gaacacaaag tacgacgaaa     2880

acgacaagct gatcagagaa gtcaaggtca tcacactgaa gagcaagctg gtcagcgact     2940

tcagaaagga cttccagttc tacaaggtca gagaaatcaa caactaccac cacgcacacg     3000

acgcatacct gaacgcagtc gtcggaacag cactgatcaa gaagtacccg aagctggaaa     3060

gcgaattcgt ctacggagac tacaaggtct acgacgtcag aaagatgatc gcaaagagcg     3120

aacaggaaat cggaaaggca acagcaaagt acttcttcta cagcaacatc atgaacttct     3180

tcaagacaga aatcacactg gcaaacggag aaatcagaaa gagaccgctg atcgaaacaa     3240

acggagaaac aggagaaatc gtctgggaca agggaagaga cttcgcaaca gtcagaaagg     3300

tcctgagcat gccgcaggtc aacatcgtca agaagacaga agtccagaca ggaggattca     3360

gcaaggaaag catcctgccg aagagaaaca gcgacaagct gatcgcaaga aagaaggact     3420

gggacccgaa gaagtacgga ggattcgaca gcccgacagt cgcatacagc gtcctggtcg     3480

tcgcaaaggt cgaaaaggga aagagcaaga agctgaagag cgtcaaggaa ctgctgggaa     3540

tcacaatcat ggaaagaagc agcttcgaaa agaacccgat cgacttcctg gaagcaaagg     3600

gatacaagga agtcaagaag gacctgatca tcaagctgcc gaagtacagc ctgttcgaac     3660

tggaaaacgg aagaaagaga atgctggcaa gcgcaggaga actgcagaag ggaaacgaac     3720

tggcactgcc gagcaagtac gtcaacttcc tgtacctggc aagccactac gaaaagctga     3780

agggaagccc ggaagacaac gaacagaagc agctgttcgt cgaacagcac aagcactacc     3840

tggacgaaat catcgaacag atcagcgaat tcagcaagag agtcatcctg gcagacgcaa     3900

acctggacaa ggtcctgagc gcatacaaca agcacagaga caagccgatc agagaacagg     3960

cagaaaacat catccacctg ttcacactga caaacctggg agcaccggca gcattcaagt     4020

acttcgacac aacaatcgac agaaagagat acacaagcac aaaggaagtc ctggacgcaa     4080

cactgatcca ccagagcatc acaggactgt acgaaacaag aatcgacctg agccagctgg     4140

gaggagacgg aggaggaagc ccgaagaaga agagaaaggt ctagctagca ccagcctcaa     4200

gaacacccga atggagtctc taagctacat aataccaact tacactttac aaaatgttgt     4260

cccccaaaat gtagccattc gtatctgctc ctaataaaaa gaaagtttct tcacattctc     4320

tcgag                                                                 4325


<210>  260
<211>  4325
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript with AGG as first three nucleotides 
       for use with CleanCapTM, 5 UTR from XBG, ORF corresponding to SEQ
       ID NO: 204, Kozak sequence, and 3 UTR of XBG

<400>  260
aggaagctca gaataaacgc tcaactttgg ccggatctgc caccatggac aagaagtaca       60

gcatcggact ggacatcgga acaaacagcg tcggatgggc agtcatcaca gacgaataca      120

aggtcccgag caagaagttc aaggtcctgg gaaacacaga cagacacagc atcaagaaga      180

acctgatcgg agcactgctg ttcgacagcg gagaaacagc agaagcaaca agactgaaga      240

gaacagcaag aagaagatac acaagaagaa agaacagaat ctgctacctg caggaaatct      300

tcagcaacga aatggcaaag gtcgacgaca gcttcttcca cagactggaa gaaagcttcc      360

tggtcgaaga agacaagaag cacgaaagac acccgatctt cggaaacatc gtcgacgaag      420

tcgcatacca cgaaaagtac ccgacaatct accacctgag aaagaagctg gtcgacagca      480

cagacaaggc agacctgaga ctgatctacc tggcactggc acacatgatc aagttcagag      540

gacacttcct gatcgaagga gacctgaacc cggacaacag cgacgtcgac aagctgttca      600

tccagctggt ccagacatac aaccagctgt tcgaagaaaa cccgatcaac gcaagcggag      660

tcgacgcaaa ggcaatcctg agcgcaagac tgagcaagag cagaagactg gaaaacctga      720

tcgcacagct gccgggagaa aagaagaacg gactgttcgg aaacctgatc gcactgagcc      780

tgggactgac accgaacttc aagagcaact tcgacctggc agaagacgca aagctgcagc      840

tgagcaagga cacatacgac gacgacctgg acaacctgct ggcacagatc ggagaccagt      900

acgcagacct gttcctggca gcaaagaacc tgagcgacgc aatcctgctg agcgacatcc      960

tgagagtcaa cacagaaatc acaaaggcac cgctgagcgc aagcatgatc aagagatacg     1020

acgaacacca ccaggacctg acactgctga aggcactggt cagacagcag ctgccggaaa     1080

agtacaagga aatcttcttc gaccagagca agaacggata cgcaggatac atcgacggag     1140

gagcaagcca ggaagaattc tacaagttca tcaagccgat cctggaaaag atggacggaa     1200

cagaagaact gctggtcaag ctgaacagag aagacctgct gagaaagcag agaacattcg     1260

acaacggaag catcccgcac cagatccacc tgggagaact gcacgcaatc ctgagaagac     1320

aggaagactt ctacccgttc ctgaaggaca acagagaaaa gatcgaaaag atcctgacat     1380

tcagaatccc gtactacgtc ggaccgctgg caagaggaaa cagcagattc gcatggatga     1440

caagaaagag cgaagaaaca atcacaccgt ggaacttcga agaagtcgtc gacaagggag     1500

caagcgcaca gagcttcatc gaaagaatga caaacttcga caagaacctg ccgaacgaaa     1560

aggtcctgcc gaagcacagc ctgctgtacg aatacttcac agtctacaac gaactgacaa     1620

aggtcaagta cgtcacagaa ggaatgagaa agccggcatt cctgagcgga gaacagaaga     1680

aggcaatcgt cgacctgctg ttcaagacaa acagaaaggt cacagtcaag cagctgaagg     1740

aagactactt caagaagatc gaatgcttcg acagcgtcga aatcagcgga gtcgaagaca     1800

gattcaacgc aagcctggga acataccacg acctgctgaa gatcatcaag gacaaggact     1860

tcctggacaa cgaagaaaac gaagacatcc tggaagacat cgtcctgaca ctgacactgt     1920

tcgaagacag agaaatgatc gaagaaagac tgaagacata cgcacacctg ttcgacgaca     1980

aggtcatgaa gcagctgaag agaagaagat acacaggatg gggaagactg agcagaaagc     2040

tgatcaacgg aatcagagac aagcagagcg gaaagacaat cctggacttc ctgaagagcg     2100

acggattcgc aaacagaaac ttcatgcagc tgatccacga cgacagcctg acattcaagg     2160

aagacatcca gaaggcacag gtcagcggac agggagacag cctgcacgaa cacatcgcaa     2220

acctggcagg aagcccggca atcaagaagg gaatcctgca gacagtcaag gtcgtcgacg     2280

aactggtcaa ggtcatggga agacacaagc cggaaaacat cgtcatcgaa atggcaagag     2340

aaaaccagac aacacagaag ggacagaaga acagcagaga aagaatgaag agaatcgaag     2400

aaggaatcaa ggaactggga agccagatcc tgaaggaaca cccggtcgaa aacacacagc     2460

tgcagaacga aaagctgtac ctgtactacc tgcagaacgg aagagacatg tacgtcgacc     2520

aggaactgga catcaacaga ctgagcgact acgacgtcga ccacatcgtc ccgcagagct     2580

tcctgaagga cgacagcatc gacaacaagg tcctgacaag aagcgacaag aacagaggaa     2640

agagcgacaa cgtcccgagc gaagaagtcg tcaagaagat gaagaactac tggagacagc     2700

tgctgaacgc aaagctgatc acacagagaa agttcgacaa cctgacaaag gcagagagag     2760

gaggactgag cgaactggac aaggcaggat tcatcaagag acagctggtc gaaacaagac     2820

agatcacaaa gcacgtcgca cagatcctgg acagcagaat gaacacaaag tacgacgaaa     2880

acgacaagct gatcagagaa gtcaaggtca tcacactgaa gagcaagctg gtcagcgact     2940

tcagaaagga cttccagttc tacaaggtca gagaaatcaa caactaccac cacgcacacg     3000

acgcatacct gaacgcagtc gtcggaacag cactgatcaa gaagtacccg aagctggaaa     3060

gcgaattcgt ctacggagac tacaaggtct acgacgtcag aaagatgatc gcaaagagcg     3120

aacaggaaat cggaaaggca acagcaaagt acttcttcta cagcaacatc atgaacttct     3180

tcaagacaga aatcacactg gcaaacggag aaatcagaaa gagaccgctg atcgaaacaa     3240

acggagaaac aggagaaatc gtctgggaca agggaagaga cttcgcaaca gtcagaaagg     3300

tcctgagcat gccgcaggtc aacatcgtca agaagacaga agtccagaca ggaggattca     3360

gcaaggaaag catcctgccg aagagaaaca gcgacaagct gatcgcaaga aagaaggact     3420

gggacccgaa gaagtacgga ggattcgaca gcccgacagt cgcatacagc gtcctggtcg     3480

tcgcaaaggt cgaaaaggga aagagcaaga agctgaagag cgtcaaggaa ctgctgggaa     3540

tcacaatcat ggaaagaagc agcttcgaaa agaacccgat cgacttcctg gaagcaaagg     3600

gatacaagga agtcaagaag gacctgatca tcaagctgcc gaagtacagc ctgttcgaac     3660

tggaaaacgg aagaaagaga atgctggcaa gcgcaggaga actgcagaag ggaaacgaac     3720

tggcactgcc gagcaagtac gtcaacttcc tgtacctggc aagccactac gaaaagctga     3780

agggaagccc ggaagacaac gaacagaagc agctgttcgt cgaacagcac aagcactacc     3840

tggacgaaat catcgaacag atcagcgaat tcagcaagag agtcatcctg gcagacgcaa     3900

acctggacaa ggtcctgagc gcatacaaca agcacagaga caagccgatc agagaacagg     3960

cagaaaacat catccacctg ttcacactga caaacctggg agcaccggca gcattcaagt     4020

acttcgacac aacaatcgac agaaagagat acacaagcac aaaggaagtc ctggacgcaa     4080

cactgatcca ccagagcatc acaggactgt acgaaacaag aatcgacctg agccagctgg     4140

gaggagacgg aggaggaagc ccgaagaaga agagaaaggt ctagctagca ccagcctcaa     4200

gaacacccga atggagtctc taagctacat aataccaact tacactttac aaaatgttgt     4260

cccccaaaat gtagccattc gtatctgctc ctaataaaaa gaaagtttct tcacattctc     4320

tcgag                                                                 4325


<210>  261
<211>  4411
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript with AGG as first three nucleotides 
       for use with CleanCapTM,  5 UTR from HSD, ORF corresponding to 
       SEQ ID NO: 204,  Kozak sequence, and 3 UTR of ALB

<400>  261
aggtcccgca gtcggcgtcc agcggctctg cttgttcgtg tgtgtgtcgt tgcaggcctt       60

attcggatcc gccaccatgg acaagaagta cagcatcgga ctggacatcg gaacaaacag      120

cgtcggatgg gcagtcatca cagacgaata caaggtcccg agcaagaagt tcaaggtcct      180

gggaaacaca gacagacaca gcatcaagaa gaacctgatc ggagcactgc tgttcgacag      240

cggagaaaca gcagaagcaa caagactgaa gagaacagca agaagaagat acacaagaag      300

aaagaacaga atctgctacc tgcaggaaat cttcagcaac gaaatggcaa aggtcgacga      360

cagcttcttc cacagactgg aagaaagctt cctggtcgaa gaagacaaga agcacgaaag      420

acacccgatc ttcggaaaca tcgtcgacga agtcgcatac cacgaaaagt acccgacaat      480

ctaccacctg agaaagaagc tggtcgacag cacagacaag gcagacctga gactgatcta      540

cctggcactg gcacacatga tcaagttcag aggacacttc ctgatcgaag gagacctgaa      600

cccggacaac agcgacgtcg acaagctgtt catccagctg gtccagacat acaaccagct      660

gttcgaagaa aacccgatca acgcaagcgg agtcgacgca aaggcaatcc tgagcgcaag      720

actgagcaag agcagaagac tggaaaacct gatcgcacag ctgccgggag aaaagaagaa      780

cggactgttc ggaaacctga tcgcactgag cctgggactg acaccgaact tcaagagcaa      840

cttcgacctg gcagaagacg caaagctgca gctgagcaag gacacatacg acgacgacct      900

ggacaacctg ctggcacaga tcggagacca gtacgcagac ctgttcctgg cagcaaagaa      960

cctgagcgac gcaatcctgc tgagcgacat cctgagagtc aacacagaaa tcacaaaggc     1020

accgctgagc gcaagcatga tcaagagata cgacgaacac caccaggacc tgacactgct     1080

gaaggcactg gtcagacagc agctgccgga aaagtacaag gaaatcttct tcgaccagag     1140

caagaacgga tacgcaggat acatcgacgg aggagcaagc caggaagaat tctacaagtt     1200

catcaagccg atcctggaaa agatggacgg aacagaagaa ctgctggtca agctgaacag     1260

agaagacctg ctgagaaagc agagaacatt cgacaacgga agcatcccgc accagatcca     1320

cctgggagaa ctgcacgcaa tcctgagaag acaggaagac ttctacccgt tcctgaagga     1380

caacagagaa aagatcgaaa agatcctgac attcagaatc ccgtactacg tcggaccgct     1440

ggcaagagga aacagcagat tcgcatggat gacaagaaag agcgaagaaa caatcacacc     1500

gtggaacttc gaagaagtcg tcgacaaggg agcaagcgca cagagcttca tcgaaagaat     1560

gacaaacttc gacaagaacc tgccgaacga aaaggtcctg ccgaagcaca gcctgctgta     1620

cgaatacttc acagtctaca acgaactgac aaaggtcaag tacgtcacag aaggaatgag     1680

aaagccggca ttcctgagcg gagaacagaa gaaggcaatc gtcgacctgc tgttcaagac     1740

aaacagaaag gtcacagtca agcagctgaa ggaagactac ttcaagaaga tcgaatgctt     1800

cgacagcgtc gaaatcagcg gagtcgaaga cagattcaac gcaagcctgg gaacatacca     1860

cgacctgctg aagatcatca aggacaagga cttcctggac aacgaagaaa acgaagacat     1920

cctggaagac atcgtcctga cactgacact gttcgaagac agagaaatga tcgaagaaag     1980

actgaagaca tacgcacacc tgttcgacga caaggtcatg aagcagctga agagaagaag     2040

atacacagga tggggaagac tgagcagaaa gctgatcaac ggaatcagag acaagcagag     2100

cggaaagaca atcctggact tcctgaagag cgacggattc gcaaacagaa acttcatgca     2160

gctgatccac gacgacagcc tgacattcaa ggaagacatc cagaaggcac aggtcagcgg     2220

acagggagac agcctgcacg aacacatcgc aaacctggca ggaagcccgg caatcaagaa     2280

gggaatcctg cagacagtca aggtcgtcga cgaactggtc aaggtcatgg gaagacacaa     2340

gccggaaaac atcgtcatcg aaatggcaag agaaaaccag acaacacaga agggacagaa     2400

gaacagcaga gaaagaatga agagaatcga agaaggaatc aaggaactgg gaagccagat     2460

cctgaaggaa cacccggtcg aaaacacaca gctgcagaac gaaaagctgt acctgtacta     2520

cctgcagaac ggaagagaca tgtacgtcga ccaggaactg gacatcaaca gactgagcga     2580

ctacgacgtc gaccacatcg tcccgcagag cttcctgaag gacgacagca tcgacaacaa     2640

ggtcctgaca agaagcgaca agaacagagg aaagagcgac aacgtcccga gcgaagaagt     2700

cgtcaagaag atgaagaact actggagaca gctgctgaac gcaaagctga tcacacagag     2760

aaagttcgac aacctgacaa aggcagagag aggaggactg agcgaactgg acaaggcagg     2820

attcatcaag agacagctgg tcgaaacaag acagatcaca aagcacgtcg cacagatcct     2880

ggacagcaga atgaacacaa agtacgacga aaacgacaag ctgatcagag aagtcaaggt     2940

catcacactg aagagcaagc tggtcagcga cttcagaaag gacttccagt tctacaaggt     3000

cagagaaatc aacaactacc accacgcaca cgacgcatac ctgaacgcag tcgtcggaac     3060

agcactgatc aagaagtacc cgaagctgga aagcgaattc gtctacggag actacaaggt     3120

ctacgacgtc agaaagatga tcgcaaagag cgaacaggaa atcggaaagg caacagcaaa     3180

gtacttcttc tacagcaaca tcatgaactt cttcaagaca gaaatcacac tggcaaacgg     3240

agaaatcaga aagagaccgc tgatcgaaac aaacggagaa acaggagaaa tcgtctggga     3300

caagggaaga gacttcgcaa cagtcagaaa ggtcctgagc atgccgcagg tcaacatcgt     3360

caagaagaca gaagtccaga caggaggatt cagcaaggaa agcatcctgc cgaagagaaa     3420

cagcgacaag ctgatcgcaa gaaagaagga ctgggacccg aagaagtacg gaggattcga     3480

cagcccgaca gtcgcataca gcgtcctggt cgtcgcaaag gtcgaaaagg gaaagagcaa     3540

gaagctgaag agcgtcaagg aactgctggg aatcacaatc atggaaagaa gcagcttcga     3600

aaagaacccg atcgacttcc tggaagcaaa gggatacaag gaagtcaaga aggacctgat     3660

catcaagctg ccgaagtaca gcctgttcga actggaaaac ggaagaaaga gaatgctggc     3720

aagcgcagga gaactgcaga agggaaacga actggcactg ccgagcaagt acgtcaactt     3780

cctgtacctg gcaagccact acgaaaagct gaagggaagc ccggaagaca acgaacagaa     3840

gcagctgttc gtcgaacagc acaagcacta cctggacgaa atcatcgaac agatcagcga     3900

attcagcaag agagtcatcc tggcagacgc aaacctggac aaggtcctga gcgcatacaa     3960

caagcacaga gacaagccga tcagagaaca ggcagaaaac atcatccacc tgttcacact     4020

gacaaacctg ggagcaccgg cagcattcaa gtacttcgac acaacaatcg acagaaagag     4080

atacacaagc acaaaggaag tcctggacgc aacactgatc caccagagca tcacaggact     4140

gtacgaaaca agaatcgacc tgagccagct gggaggagac ggaggaggaa gcccgaagaa     4200

gaagagaaag gtctagctag ccatcacatt taaaagcatc tcagcctacc atgagaataa     4260

gagaaagaaa atgaagatca atagcttatt catctctttt tctttttcgt tggtgtaaag     4320

ccaacaccct gtctaaaaaa cataaatttc tttaatcatt ttgcctcttt tctctgtgct     4380

tcaattaata aaaaatggaa agaacctcga g                                    4411


<210>  262
<211>  105
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 30/30/39 poly-A sequence

<400>  262
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gcgaaaaaaa aaaaaaaaaa aaaaaaaaaa       60

aaaccgaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa                      105


<210>  263
<211>  93
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: poly-A 100 sequence

<400>  263
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa       60

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa                                    93


<210>  264
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G209 guide RNA


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  264
ccaguccagc gaggcaaagg guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  265

<400>  265
000

<210>  266

<400>  266
000

<210>  267

<400>  267
000

<210>  268
<211>  1103
<212>  PRT
<213>  Neisseria meningitidis


<220>
<221>  misc_feature
<223>  Amino acid sequence of Neisseria meningitidis Cas9

<400>  268

Met Ala Ala Phe Lys Pro Asn Ser Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Glu 
            20                  25                  30          


Glu Asn Pro Ile Arg Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Thr Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asn 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Gly Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Ser Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Ile Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Val Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Ile Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Leu Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Gln Gly Lys Asp Glu Glu Asp Trp Gln Leu Ile Asp 
            980                 985                 990         


Asp Ser Phe Asn Phe Lys Phe Ser  Leu His Pro Asn Asp  Leu Val Glu 
        995                 1000                 1005             


Val Ile  Thr Lys Lys Ala Arg  Met Phe Gly Tyr Phe  Ala Ser Cys 
    1010                 1015                 1020             


His Arg  Gly Thr Gly Asn Ile  Asn Ile Arg Ile His  Asp Leu Asp 
    1025                 1030                 1035             


His Lys  Ile Gly Lys Asn Gly  Ile Leu Glu Gly Ile  Gly Val Lys 
    1040                 1045                 1050             


Thr Ala  Leu Ser Phe Gln Lys  Tyr Gln Ile Asp Glu  Leu Gly Lys 
    1055                 1060                 1065             


Glu Ile  Arg Pro Cys Arg Leu  Lys Lys Arg Pro Pro  Val Arg Ser 
    1070                 1075                 1080             


Gly Lys  Arg Thr Ala Asp Gly  Ser Glu Phe Glu Ser  Pro Lys Lys 
    1085                 1090                 1095             


Lys Arg  Lys Val Glu 
    1100             


<210>  269

<400>  269
000

<210>  270
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G502 guide RNA


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  270
acacaaauac caguccagcg guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  271

<400>  271
000

<210>  272

<400>  272
000

<210>  273
<211>  3783
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA coding sequence of eGFP

<400>  273
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ctaatacgac tcactatagg      420

gtcccgcagt cggcgtccag cggctctgct tgttcgtgtg tgtgtcgttg caggccttat      480

tcggatccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg      540

agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg      600

ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct      660

ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc      720

acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca      780

ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg      840

acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc      900

tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc      960

agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc     1020

agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg     1080

acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc     1140

acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt     1200

acaagtaata ggaattatgc agtctagcca tcacatttaa aagcatctca gcctaccatg     1260

agaataagag aaagaaaatg aagatcaata gcttattcat ctctttttct ttttcgttgg     1320

tgtaaagcca acaccctgtc taaaaaacat aaatttcttt aatcattttg cctcttttct     1380

ctgtgcttca attaataaaa aatggaaaga acctcgagaa aaaaaaaaaa aaaaaaaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaatc tagacttaag cttgatgagc tctagcttgg cgtaatcatg     1560

gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc     1620

cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc     1680

gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat     1740

cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac     1800

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     1860

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     1920

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     1980

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     2040

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     2100

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     2160

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     2220

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     2280

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     2340

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag     2400

aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     2460

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     2520

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     2580

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag     2640

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata     2700

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat     2760

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg     2820

ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc     2880

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc     2940

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc     3000

gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc     3060

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc     3120

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa     3180

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat     3240

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata     3300

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca     3360

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag     3420

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc     3480

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc     3540

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata     3600

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta     3660

gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta     3720

agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg     3780

tcg                                                                   3783


<210>  274
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Modified sgRNA pattern, where N are nucleotides 
       encoding a guide sequence


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  274
nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  275
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CMV-1 5 UTR

<400>  275
cagatcgcct ggagacgcca tccacgctgt tttgacctcc at                          42


<210>  276
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CMV-2 5 UTR

<400>  276
agaagacacc gggaccgatc cagcctccgc ggccgggaac gg                          42


<210>  277
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CMV-3 5 UTR

<400>  277
tgcattggaa cgcggattcc ccgtgccaag agtgactcac cg                          42


<210>  278
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: SV40 NLS

<400>  278

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  279
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS 1

<400>  279

Leu Ala Ala Lys Arg Ser Arg Thr Thr 
1               5                   


<210>  280
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS 2

<400>  280

Gln Ala Ala Lys Arg Ser Arg Thr Thr 
1               5                   


<210>  281
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS 3

<400>  281

Pro Ala Pro Ala Lys Arg Glu Arg Thr Thr 
1               5                   10  


<210>  282
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS 4

<400>  282

Gln Ala Ala Lys Arg Pro Arg Thr Thr 
1               5                   


<210>  283
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS 5

<400>  283

Arg Ala Ala Lys Arg Pro Arg Thr Thr 
1               5                   


<210>  284
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS 6

<400>  284

Ala Ala Ala Lys Arg Ser Trp Ser Met Ala Ala 
1               5                   10      


<210>  285
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS 7

<400>  285

Ala Ala Ala Lys Arg Val Trp Ser Met Ala Phe 
1               5                   10      


<210>  286
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS 8

<400>  286

Ala Ala Ala Lys Arg Ser Trp Ser Met Ala Phe 
1               5                   10      


<210>  287
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS 9

<400>  287

Ala Ala Ala Lys Arg Lys Tyr Phe Ala Ala 
1               5                   10  


<210>  288
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS 10

<400>  288

Arg Ala Ala Lys Arg Lys Ala Phe Ala Ala 
1               5                   10  


<210>  289
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS 11

<400>  289

Arg Ala Ala Lys Arg Lys Tyr Phe Ala Val 
1               5                   10  


<210>  290
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Alternate SV40 NLS

<400>  290

Pro Lys Lys Lys Arg Arg Val 
1               5           


<210>  291
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nucleoplasmin NLS

<400>  291

Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


<210>  292
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary coding sequence for SV40 NLS

<400>  292
ccgaagaaga agagaaaggt c                                                 21


<210>  293
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary coding sequence for NLS1

<400>  293
ctggcagcaa agagaagcag aacaaca                                           27


<210>  294
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary coding sequence for NLS2

<400>  294
caggcagcaa agagaagcag aacaaca                                           27


<210>  295
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary coding sequence for NLS3

<400>  295
ccggcaccgg caaagagaga aagaacaaca                                        30


<210>  296
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary coding sequence for NLS4

<400>  296
caggcagcaa agagaccgag aacaaca                                           27


<210>  297
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary coding sequence for NLS5

<400>  297
agagcagcaa agagaccgag aacaaca                                           27


<210>  298
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary coding sequence for NLS6

<400>  298
gcagcagcaa agagaagctg gagcatggca gca                                    33


<210>  299
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary coding sequence for NLS7

<400>  299
gcagcagcaa agagagtctg gagcatggca ttc                                    33


<210>  300
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary coding sequence for NLS8

<400>  300
gcagcagcaa agagaagctg gagcatggca ttc                                    33


<210>  301
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary coding sequence for NLS9

<400>  301
gcagcagcaa agagaaagta cttcgcagca                                        30


<210>  302
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary coding sequence for NLS10

<400>  302
agagcagcaa agagaaaggc attcgcagca                                        30


<210>  303
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary coding sequence for NLS11

<400>  303
agagcagcaa agagaaagta cttcgcagtc                                        30


<210>  304
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary coding sequence for alternate SV40 NLS

<400>  304
ccgaagaaga agagaagagt c                                                 21


<210>  305
<211>  13
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: exemplary Kozak sequence

<400>  305
gccgccrcca ugg                                                          13


<210>  306

<400>  306
000

<210>  307

<400>  307
000

<210>  308

<400>  308
000

<210>  309

<400>  309
000

<210>  310

<400>  310
000

<210>  311
<211>  4140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 ORF using low A codons of Table 5, with start and
       stop codons

<400>  311
atggacaaga agtactccat cggcctggac atcggcacca actccgtggg ctgggccgtg       60

atcaccgacg agtacaaggt gccctccaag aagttcaagg tgctgggcaa caccgaccgg      120

cactccatca agaagaacct gatcggcgcc ctgctgttcg actccggcga gaccgccgag      180

gccacccggc tgaagcggac cgcccggcgg cggtacaccc ggcggaagaa ccggatctgc      240

tacctgcagg agatcttctc caacgagatg gccaaggtgg acgactcctt cttccaccgg      300

ctggaggagt ccttcctggt ggaggaggac aagaagcacg agcggcaccc catcttcggc      360

aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgcggaag      420

aagctggtgg actccaccga caaggccgac ctgcggctga tctacctggc cctggcccac      480

atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caactccgac      540

gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggagaacccc      600

atcaacgcct ccggcgtgga cgccaaggcc atcctgtccg cccggctgtc caagtcccgg      660

cggctggaga acctgatcgc ccagctgccc ggcgagaaga agaacggcct gttcggcaac      720

ctgatcgccc tgtccctggg cctgaccccc aacttcaagt ccaacttcga cctggccgag      780

gacgccaagc tgcagctgtc caaggacacc tacgacgacg acctggacaa cctgctggcc      840

cagatcggcg accagtacgc cgacctgttc ctggccgcca agaacctgtc cgacgccatc      900

ctgctgtccg acatcctgcg ggtgaacacc gagatcacca aggcccccct gtccgcctcc      960

atgatcaagc ggtacgacga gcaccaccag gacctgaccc tgctgaaggc cctggtgcgg     1020

cagcagctgc ccgagaagta caaggagatc ttcttcgacc agtccaagaa cggctacgcc     1080

ggctacatcg acggcggcgc ctcccaggag gagttctaca agttcatcaa gcccatcctg     1140

gagaagatgg acggcaccga ggagctgctg gtgaagctga accgggagga cctgctgcgg     1200

aagcagcgga ccttcgacaa cggctccatc ccccaccaga tccacctggg cgagctgcac     1260

gccatcctgc ggcggcagga ggacttctac cccttcctga aggacaaccg ggagaagatc     1320

gagaagatcc tgaccttccg gatcccctac tacgtgggcc ccctggcccg gggcaactcc     1380

cggttcgcct ggatgacccg gaagtccgag gagaccatca ccccctggaa cttcgaggag     1440

gtggtggaca agggcgcctc cgcccagtcc ttcatcgagc ggatgaccaa cttcgacaag     1500

aacctgccca acgagaaggt gctgcccaag cactccctgc tgtacgagta cttcaccgtg     1560

tacaacgagc tgaccaaggt gaagtacgtg accgagggca tgcggaagcc cgccttcctg     1620

tccggcgagc agaagaaggc catcgtggac ctgctgttca agaccaaccg gaaggtgacc     1680

gtgaagcagc tgaaggagga ctacttcaag aagatcgagt gcttcgactc cgtggagatc     1740

tccggcgtgg aggaccggtt caacgcctcc ctgggcacct accacgacct gctgaagatc     1800

atcaaggaca aggacttcct ggacaacgag gagaacgagg acatcctgga ggacatcgtg     1860

ctgaccctga ccctgttcga ggaccgggag atgatcgagg agcggctgaa gacctacgcc     1920

cacctgttcg acgacaaggt gatgaagcag ctgaagcggc ggcggtacac cggctggggc     1980

cggctgtccc ggaagctgat caacggcatc cgggacaagc agtccggcaa gaccatcctg     2040

gacttcctga agtccgacgg cttcgccaac cggaacttca tgcagctgat ccacgacgac     2100

tccctgacct tcaaggagga catccagaag gcccaggtgt ccggccaggg cgactccctg     2160

cacgagcaca tcgccaacct ggccggctcc cccgccatca agaagggcat cctgcagacc     2220

gtgaaggtgg tggacgagct ggtgaaggtg atgggccggc acaagcccga gaacatcgtg     2280

atcgagatgg cccgggagaa ccagaccacc cagaagggcc agaagaactc ccgggagcgg     2340

atgaagcgga tcgaggaggg catcaaggag ctgggctccc agatcctgaa ggagcacccc     2400

gtggagaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaacggccgg     2460

gacatgtacg tggaccagga gctggacatc aaccggctgt ccgactacga cgtggaccac     2520

atcgtgcccc agtccttcct gaaggacgac tccatcgaca acaaggtgct gacccggtcc     2580

gacaagaacc ggggcaagtc cgacaacgtg ccctccgagg aggtggtgaa gaagatgaag     2640

aactactggc ggcagctgct gaacgccaag ctgatcaccc agcggaagtt cgacaacctg     2700

accaaggccg agcggggcgg cctgtccgag ctggacaagg ccggcttcat caagcggcag     2760

ctggtggaga cccggcagat caccaagcac gtggcccaga tcctggactc ccggatgaac     2820

accaagtacg acgagaacga caagctgatc cgggaggtga aggtgatcac cctgaagtcc     2880

aagctggtgt ccgacttccg gaaggacttc cagttctaca aggtgcggga gatcaacaac     2940

taccaccacg cccacgacgc ctacctgaac gccgtggtgg gcaccgccct gatcaagaag     3000

taccccaagc tggagtccga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag     3060

atgatcgcca agtccgagca ggagatcggc aaggccaccg ccaagtactt cttctactcc     3120

aacatcatga acttcttcaa gaccgagatc accctggcca acggcgagat ccggaagcgg     3180

cccctgatcg agaccaacgg cgagaccggc gagatcgtgt gggacaaggg ccgggacttc     3240

gccaccgtgc ggaaggtgct gtccatgccc caggtgaaca tcgtgaagaa gaccgaggtg     3300

cagaccggcg gcttctccaa ggagtccatc ctgcccaagc ggaactccga caagctgatc     3360

gcccggaaga aggactggga ccccaagaag tacggcggct tcgactcccc caccgtggcc     3420

tactccgtgc tggtggtggc caaggtggag aagggcaagt ccaagaagct gaagtccgtg     3480

aaggagctgc tgggcatcac catcatggag cggtcctcct tcgagaagaa ccccatcgac     3540

ttcctggagg ccaagggcta caaggaggtg aagaaggacc tgatcatcaa gctgcccaag     3600

tactccctgt tcgagctgga gaacggccgg aagcggatgc tggcctccgc cggcgagctg     3660

cagaagggca acgagctggc cctgccctcc aagtacgtga acttcctgta cctggcctcc     3720

cactacgaga agctgaaggg ctcccccgag gacaacgagc agaagcagct gttcgtggag     3780

cagcacaagc actacctgga cgagatcatc gagcagatct ccgagttctc caagcgggtg     3840

atcctggccg acgccaacct ggacaaggtg ctgtccgcct acaacaagca ccgggacaag     3900

cccatccggg agcaggccga gaacatcatc cacctgttca ccctgaccaa cctgggcgcc     3960

cccgccgcct tcaagtactt cgacaccacc atcgaccgga agcggtacac ctccaccaag     4020

gaggtgctgg acgccaccct gatccaccag tccatcaccg gcctgtacga gacccggatc     4080

gacctgtccc agctgggcgg cgacggcggc ggctccccca agaagaagcg gaaggtgtga     4140


<210>  312
<211>  4140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 ORF using low A/U codons of Table 5, with start 
       and stop codons

<400>  312
atggacaaga agtacagcat cggcctggac atcggcacca acagcgtggg ctgggccgtg       60

atcaccgacg agtacaaggt gcccagcaag aagttcaagg tgctgggcaa caccgaccgg      120

cacagcatca agaagaacct gatcggcgcc ctgctgttcg acagcggcga gaccgccgag      180

gccacccggc tgaagcggac cgcccggcgg cggtacaccc ggcggaagaa ccggatctgc      240

tacctgcagg agatcttcag caacgagatg gccaaggtgg acgacagctt cttccaccgg      300

ctggaggaga gcttcctggt ggaggaggac aagaagcacg agcggcaccc catcttcggc      360

aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgcggaag      420

aagctggtgg acagcaccga caaggccgac ctgcggctga tctacctggc cctggcccac      480

atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac      540

gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggagaacccc      600

atcaacgcca gcggcgtgga cgccaaggcc atcctgagcg cccggctgag caagagccgg      660

cggctggaga acctgatcgc ccagctgccc ggcgagaaga agaacggcct gttcggcaac      720

ctgatcgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag      780

gacgccaagc tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc      840

cagatcggcg accagtacgc cgacctgttc ctggccgcca agaacctgag cgacgccatc      900

ctgctgagcg acatcctgcg ggtgaacacc gagatcacca aggcccccct gagcgccagc      960

atgatcaagc ggtacgacga gcaccaccag gacctgaccc tgctgaaggc cctggtgcgg     1020

cagcagctgc ccgagaagta caaggagatc ttcttcgacc agagcaagaa cggctacgcc     1080

ggctacatcg acggcggcgc cagccaggag gagttctaca agttcatcaa gcccatcctg     1140

gagaagatgg acggcaccga ggagctgctg gtgaagctga accgggagga cctgctgcgg     1200

aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg cgagctgcac     1260

gccatcctgc ggcggcagga ggacttctac cccttcctga aggacaaccg ggagaagatc     1320

gagaagatcc tgaccttccg gatcccctac tacgtgggcc ccctggcccg gggcaacagc     1380

cggttcgcct ggatgacccg gaagagcgag gagaccatca ccccctggaa cttcgaggag     1440

gtggtggaca agggcgccag cgcccagagc ttcatcgagc ggatgaccaa cttcgacaag     1500

aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg     1560

tacaacgagc tgaccaaggt gaagtacgtg accgagggca tgcggaagcc cgccttcctg     1620

agcggcgagc agaagaaggc catcgtggac ctgctgttca agaccaaccg gaaggtgacc     1680

gtgaagcagc tgaaggagga ctacttcaag aagatcgagt gcttcgacag cgtggagatc     1740

agcggcgtgg aggaccggtt caacgccagc ctgggcacct accacgacct gctgaagatc     1800

atcaaggaca aggacttcct ggacaacgag gagaacgagg acatcctgga ggacatcgtg     1860

ctgaccctga ccctgttcga ggaccgggag atgatcgagg agcggctgaa gacctacgcc     1920

cacctgttcg acgacaaggt gatgaagcag ctgaagcggc ggcggtacac cggctggggc     1980

cggctgagcc ggaagctgat caacggcatc cgggacaagc agagcggcaa gaccatcctg     2040

gacttcctga agagcgacgg cttcgccaac cggaacttca tgcagctgat ccacgacgac     2100

agcctgacct tcaaggagga catccagaag gcccaggtga gcggccaggg cgacagcctg     2160

cacgagcaca tcgccaacct ggccggcagc cccgccatca agaagggcat cctgcagacc     2220

gtgaaggtgg tggacgagct ggtgaaggtg atgggccggc acaagcccga gaacatcgtg     2280

atcgagatgg cccgggagaa ccagaccacc cagaagggcc agaagaacag ccgggagcgg     2340

atgaagcgga tcgaggaggg catcaaggag ctgggcagcc agatcctgaa ggagcacccc     2400

gtggagaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaacggccgg     2460

gacatgtacg tggaccagga gctggacatc aaccggctga gcgactacga cgtggaccac     2520

atcgtgcccc agagcttcct gaaggacgac agcatcgaca acaaggtgct gacccggagc     2580

gacaagaacc ggggcaagag cgacaacgtg cccagcgagg aggtggtgaa gaagatgaag     2640

aactactggc ggcagctgct gaacgccaag ctgatcaccc agcggaagtt cgacaacctg     2700

accaaggccg agcggggcgg cctgagcgag ctggacaagg ccggcttcat caagcggcag     2760

ctggtggaga cccggcagat caccaagcac gtggcccaga tcctggacag ccggatgaac     2820

accaagtacg acgagaacga caagctgatc cgggaggtga aggtgatcac cctgaagagc     2880

aagctggtga gcgacttccg gaaggacttc cagttctaca aggtgcggga gatcaacaac     2940

taccaccacg cccacgacgc ctacctgaac gccgtggtgg gcaccgccct gatcaagaag     3000

taccccaagc tggagagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag     3060

atgatcgcca agagcgagca ggagatcggc aaggccaccg ccaagtactt cttctacagc     3120

aacatcatga acttcttcaa gaccgagatc accctggcca acggcgagat ccggaagcgg     3180

cccctgatcg agaccaacgg cgagaccggc gagatcgtgt gggacaaggg ccgggacttc     3240

gccaccgtgc ggaaggtgct gagcatgccc caggtgaaca tcgtgaagaa gaccgaggtg     3300

cagaccggcg gcttcagcaa ggagagcatc ctgcccaagc ggaacagcga caagctgatc     3360

gcccggaaga aggactggga ccccaagaag tacggcggct tcgacagccc caccgtggcc     3420

tacagcgtgc tggtggtggc caaggtggag aagggcaaga gcaagaagct gaagagcgtg     3480

aaggagctgc tgggcatcac catcatggag cggagcagct tcgagaagaa ccccatcgac     3540

ttcctggagg ccaagggcta caaggaggtg aagaaggacc tgatcatcaa gctgcccaag     3600

tacagcctgt tcgagctgga gaacggccgg aagcggatgc tggccagcgc cggcgagctg     3660

cagaagggca acgagctggc cctgcccagc aagtacgtga acttcctgta cctggccagc     3720

cactacgaga agctgaaggg cagccccgag gacaacgagc agaagcagct gttcgtggag     3780

cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttcag caagcgggtg     3840

atcctggccg acgccaacct ggacaaggtg ctgagcgcct acaacaagca ccgggacaag     3900

cccatccggg agcaggccga gaacatcatc cacctgttca ccctgaccaa cctgggcgcc     3960

cccgccgcct tcaagtactt cgacaccacc atcgaccgga agcggtacac cagcaccaag     4020

gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacccggatc     4080

gacctgagcc agctgggcgg cgacggcggc ggcagcccca agaagaagcg gaaggtgtga     4140


<210>  313
<211>  4179
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 ORF using low A codons of Table 5, with two 
       C-terminal NLS sequences and start and stop codons

<400>  313
atggacaaga agtactccat cggcctggac atcggcacca actccgtggg ctgggccgtg       60

atcaccgacg agtacaaggt gccctccaag aagttcaagg tgctgggcaa caccgaccgg      120

cactccatca agaagaacct gatcggcgcc ctgctgttcg actccggcga gaccgccgag      180

gccacccggc tgaagcggac cgcccggcgg cggtacaccc ggcggaagaa ccggatctgc      240

tacctgcagg agatcttctc caacgagatg gccaaggtgg acgactcctt cttccaccgg      300

ctggaggagt ccttcctggt ggaggaggac aagaagcacg agcggcaccc catcttcggc      360

aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgcggaag      420

aagctggtgg actccaccga caaggccgac ctgcggctga tctacctggc cctggcccac      480

atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caactccgac      540

gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggagaacccc      600

atcaacgcct ccggcgtgga cgccaaggcc atcctgtccg cccggctgtc caagtcccgg      660

cggctggaga acctgatcgc ccagctgccc ggcgagaaga agaacggcct gttcggcaac      720

ctgatcgccc tgtccctggg cctgaccccc aacttcaagt ccaacttcga cctggccgag      780

gacgccaagc tgcagctgtc caaggacacc tacgacgacg acctggacaa cctgctggcc      840

cagatcggcg accagtacgc cgacctgttc ctggccgcca agaacctgtc cgacgccatc      900

ctgctgtccg acatcctgcg ggtgaacacc gagatcacca aggcccccct gtccgcctcc      960

atgatcaagc ggtacgacga gcaccaccag gacctgaccc tgctgaaggc cctggtgcgg     1020

cagcagctgc ccgagaagta caaggagatc ttcttcgacc agtccaagaa cggctacgcc     1080

ggctacatcg acggcggcgc ctcccaggag gagttctaca agttcatcaa gcccatcctg     1140

gagaagatgg acggcaccga ggagctgctg gtgaagctga accgggagga cctgctgcgg     1200

aagcagcgga ccttcgacaa cggctccatc ccccaccaga tccacctggg cgagctgcac     1260

gccatcctgc ggcggcagga ggacttctac cccttcctga aggacaaccg ggagaagatc     1320

gagaagatcc tgaccttccg gatcccctac tacgtgggcc ccctggcccg gggcaactcc     1380

cggttcgcct ggatgacccg gaagtccgag gagaccatca ccccctggaa cttcgaggag     1440

gtggtggaca agggcgcctc cgcccagtcc ttcatcgagc ggatgaccaa cttcgacaag     1500

aacctgccca acgagaaggt gctgcccaag cactccctgc tgtacgagta cttcaccgtg     1560

tacaacgagc tgaccaaggt gaagtacgtg accgagggca tgcggaagcc cgccttcctg     1620

tccggcgagc agaagaaggc catcgtggac ctgctgttca agaccaaccg gaaggtgacc     1680

gtgaagcagc tgaaggagga ctacttcaag aagatcgagt gcttcgactc cgtggagatc     1740

tccggcgtgg aggaccggtt caacgcctcc ctgggcacct accacgacct gctgaagatc     1800

atcaaggaca aggacttcct ggacaacgag gagaacgagg acatcctgga ggacatcgtg     1860

ctgaccctga ccctgttcga ggaccgggag atgatcgagg agcggctgaa gacctacgcc     1920

cacctgttcg acgacaaggt gatgaagcag ctgaagcggc ggcggtacac cggctggggc     1980

cggctgtccc ggaagctgat caacggcatc cgggacaagc agtccggcaa gaccatcctg     2040

gacttcctga agtccgacgg cttcgccaac cggaacttca tgcagctgat ccacgacgac     2100

tccctgacct tcaaggagga catccagaag gcccaggtgt ccggccaggg cgactccctg     2160

cacgagcaca tcgccaacct ggccggctcc cccgccatca agaagggcat cctgcagacc     2220

gtgaaggtgg tggacgagct ggtgaaggtg atgggccggc acaagcccga gaacatcgtg     2280

atcgagatgg cccgggagaa ccagaccacc cagaagggcc agaagaactc ccgggagcgg     2340

atgaagcgga tcgaggaggg catcaaggag ctgggctccc agatcctgaa ggagcacccc     2400

gtggagaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaacggccgg     2460

gacatgtacg tggaccagga gctggacatc aaccggctgt ccgactacga cgtggaccac     2520

atcgtgcccc agtccttcct gaaggacgac tccatcgaca acaaggtgct gacccggtcc     2580

gacaagaacc ggggcaagtc cgacaacgtg ccctccgagg aggtggtgaa gaagatgaag     2640

aactactggc ggcagctgct gaacgccaag ctgatcaccc agcggaagtt cgacaacctg     2700

accaaggccg agcggggcgg cctgtccgag ctggacaagg ccggcttcat caagcggcag     2760

ctggtggaga cccggcagat caccaagcac gtggcccaga tcctggactc ccggatgaac     2820

accaagtacg acgagaacga caagctgatc cgggaggtga aggtgatcac cctgaagtcc     2880

aagctggtgt ccgacttccg gaaggacttc cagttctaca aggtgcggga gatcaacaac     2940

taccaccacg cccacgacgc ctacctgaac gccgtggtgg gcaccgccct gatcaagaag     3000

taccccaagc tggagtccga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag     3060

atgatcgcca agtccgagca ggagatcggc aaggccaccg ccaagtactt cttctactcc     3120

aacatcatga acttcttcaa gaccgagatc accctggcca acggcgagat ccggaagcgg     3180

cccctgatcg agaccaacgg cgagaccggc gagatcgtgt gggacaaggg ccgggacttc     3240

gccaccgtgc ggaaggtgct gtccatgccc caggtgaaca tcgtgaagaa gaccgaggtg     3300

cagaccggcg gcttctccaa ggagtccatc ctgcccaagc ggaactccga caagctgatc     3360

gcccggaaga aggactggga ccccaagaag tacggcggct tcgactcccc caccgtggcc     3420

tactccgtgc tggtggtggc caaggtggag aagggcaagt ccaagaagct gaagtccgtg     3480

aaggagctgc tgggcatcac catcatggag cggtcctcct tcgagaagaa ccccatcgac     3540

ttcctggagg ccaagggcta caaggaggtg aagaaggacc tgatcatcaa gctgcccaag     3600

tactccctgt tcgagctgga gaacggccgg aagcggatgc tggcctccgc cggcgagctg     3660

cagaagggca acgagctggc cctgccctcc aagtacgtga acttcctgta cctggcctcc     3720

cactacgaga agctgaaggg ctcccccgag gacaacgagc agaagcagct gttcgtggag     3780

cagcacaagc actacctgga cgagatcatc gagcagatct ccgagttctc caagcgggtg     3840

atcctggccg acgccaacct ggacaaggtg ctgtccgcct acaacaagca ccgggacaag     3900

cccatccggg agcaggccga gaacatcatc cacctgttca ccctgaccaa cctgggcgcc     3960

cccgccgcct tcaagtactt cgacaccacc atcgaccgga agcggtacac ctccaccaag     4020

gaggtgctgg acgccaccct gatccaccag tccatcaccg gcctgtacga gacccggatc     4080

gacctgtccc agctgggcgg cgacggctcc ggctccccca agaagaagcg gaaggtggac     4140

ggctccccca agaagaagcg gaaggtggac tccggctga                            4179


<210>  314

<400>  314
000

<210>  315

<400>  315
000

<210>  316

<400>  316
000

<210>  317

<400>  317
000

<210>  318

<400>  318
000

<210>  319

<400>  319
000

<210>  320

<400>  320
000

<210>  321

<400>  321
000

<210>  322

<400>  322
000

<210>  323

<400>  323
000

<210>  324

<400>  324
000

<210>  325

<400>  325
000

<210>  326

<400>  326
000

<210>  327

<400>  327
000

<210>  328
<211>  3312
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nme Cas9 ORF using low A codons of Table 5, with start
       and stop codons

<400>  328
atggccgcct tcaagcccaa ctccatcaac tacatcctgg gcctggacat cggcatcgcc       60

tccgtgggct gggccatggt ggagatcgac gaggaggaga accccatccg gctgatcgac      120

ctgggcgtgc gggtgttcga gcgggccgag gtgcccaaga ccggcgactc cctggccatg      180

gcccggcggc tggcccggtc cgtgcggcgg ctgacccggc ggcgggccca ccggctgctg      240

cggacccggc ggctgctgaa gcgggagggc gtgctgcagg ccgccaactt cgacgagaac      300

ggcctgatca agtccctgcc caacaccccc tggcagctgc gggccgccgc cctggaccgg      360

aagctgaccc ccctggagtg gtccgccgtg ctgctgcacc tgatcaagca ccggggctac      420

ctgtcccagc ggaagaacga gggcgagacc gccgacaagg agctgggcgc cctgctgaag      480

ggcgtggccg gcaacgccca cgccctgcag accggcgact tccggacccc cgccgagctg      540

gccctgaaca agttcgagaa ggagtccggc cacatccgga accagcggtc cgactactcc      600

cacaccttct cccggaagga cctgcaggcc gagctgatcc tgctgttcga gaagcagaag      660

gagttcggca acccccacgt gtccggcggc ctgaaggagg gcatcgagac cctgctgatg      720

acccagcggc ccgccctgtc cggcgacgcc gtgcagaaga tgctgggcca ctgcaccttc      780

gagcccgccg agcccaaggc cgccaagaac acctacaccg ccgagcggtt catctggctg      840

accaagctga acaacctgcg gatcctggag cagggctccg agcggcccct gaccgacacc      900

gagcgggcca ccctgatgga cgagccctac cggaagtcca agctgaccta cgcccaggcc      960

cggaagctgc tgggcctgga ggacaccgcc ttcttcaagg gcctgcggta cggcaaggac     1020

aacgccgagg cctccaccct gatggagatg aaggcctacc acgccatctc ccgggccctg     1080

gagaaggagg gcctgaagga caagaagtcc cccctgaacc tgtcccccga gctgcaggac     1140

gagatcggca ccgccttctc cctgttcaag accgacgagg acatcaccgg ccggctgaag     1200

gaccggatcc agcccgagat cctggaggcc ctgctgaagc acatctcctt cgacaagttc     1260

gtgcagatct ccctgaaggc cctgcggcgg atcgtgcccc tgatggagca gggcaagcgg     1320

tacgacgagg cctgcgccga gatctacggc gaccactacg gcaagaagaa caccgaggag     1380

aagatctacc tgccccccat ccccgccgac gagatccgga accccgtggt gctgcgggcc     1440

ctgtcccagg cccggaaggt gatcaacggc gtggtgcggc ggtacggctc ccccgcccgg     1500

atccacatcg agaccgcccg ggaggtgggc aagtccttca aggaccggaa ggagatcgag     1560

aagcggcagg aggagaaccg gaaggaccgg gagaaggccg ccgccaagtt ccgggagtac     1620

ttccccaact tcgtgggcga gcccaagtcc aaggacatcc tgaagctgcg gctgtacgag     1680

cagcagcacg gcaagtgcct gtactccggc aaggagatca acctgggccg gctgaacgag     1740

aagggctacg tggagatcga ccacgccctg cccttctccc ggacctggga cgactccttc     1800

aacaacaagg tgctggtgct gggctccgag aaccagaaca agggcaacca gaccccctac     1860

gagtacttca acggcaagga caactcccgg gagtggcagg agttcaaggc ccgggtggag     1920

acctcccggt tcccccggtc caagaagcag cggatcctgc tgcagaagtt cgacgaggac     1980

ggcttcaagg agcggaacct gaacgacacc cggtacgtga accggttcct gtgccagttc     2040

gtggccgacc ggatgcggct gaccggcaag ggcaagaagc gggtgttcgc ctccaacggc     2100

cagatcacca acctgctgcg gggcttctgg ggcctgcgga aggtgcgggc cgagaacgac     2160

cggcaccacg ccctggacgc cgtggtggtg gcctgctcca ccgtggccat gcagcagaag     2220

atcacccggt tcgtgcggta caaggagatg aacgccttcg acggcaagac catcgacaag     2280

gagaccggcg aggtgctgca ccagaagacc cacttccccc agccctggga gttcttcgcc     2340

caggaggtga tgatccgggt gttcggcaag cccgacggca agcccgagtt cgaggaggcc     2400

gacaccctgg agaagctgcg gaccctgctg gccgagaagc tgtcctcccg gcccgaggcc     2460

gtgcacgagt acgtgacccc cctgttcgtg tcccgggccc ccaaccggaa gatgtccggc     2520

cagggccaca tggagaccgt gaagtccgcc aagcggctgg acgagggcgt gtccgtgctg     2580

cgggtgcccc tgacccagct gaagctgaag gacctggaga agatggtgaa ccgggagcgg     2640

gagcccaagc tgtacgaggc cctgaaggcc cggctggagg cccacaagga cgaccccgcc     2700

aaggccttcg ccgagccctt ctacaagtac gacaaggccg gcaaccggac ccagcaggtg     2760

aaggccgtgc gggtggagca ggtgcagaag accggcgtgt gggtgcggaa ccacaacggc     2820

atcgccgaca acgccaccat ggtgcgggtg gacgtgttcg agaagggcga caagtactac     2880

ctggtgccca tctactcctg gcaggtggcc aagggcatcc tgcccgaccg ggccgtggtg     2940

cagggcaagg acgaggagga ctggcagctg atcgacgact ccttcaactt caagttctcc     3000

ctgcacccca acgacctggt ggaggtgatc accaagaagg cccggatgtt cggctacttc     3060

gcctcctgcc accggggcac cggcaacatc aacatccgga tccacgacct ggaccacaag     3120

atcggcaaga acggcatcct ggagggcatc ggcgtgaaga ccgccctgtc cttccagaag     3180

taccagatcg acgagctggg caaggagatc cggccctgcc ggctgaagaa gcggcccccc     3240

gtgcggtccg gcaagcggac cgccgacggc tccgagttcg agtcccccaa gaagaagcgg     3300

aaggtggagt ga                                                         3312


<210>  329
<211>  3312
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nme Cas9 ORF using low A/U codons of Table 5, with 
       start and stop codons

<400>  329
atggccgcct tcaagcccaa cagcatcaac tacatcctgg gcctggacat cggcatcgcc       60

agcgtgggct gggccatggt ggagatcgac gaggaggaga accccatccg gctgatcgac      120

ctgggcgtgc gggtgttcga gcgggccgag gtgcccaaga ccggcgacag cctggccatg      180

gcccggcggc tggcccggag cgtgcggcgg ctgacccggc ggcgggccca ccggctgctg      240

cggacccggc ggctgctgaa gcgggagggc gtgctgcagg ccgccaactt cgacgagaac      300

ggcctgatca agagcctgcc caacaccccc tggcagctgc gggccgccgc cctggaccgg      360

aagctgaccc ccctggagtg gagcgccgtg ctgctgcacc tgatcaagca ccggggctac      420

ctgagccagc ggaagaacga gggcgagacc gccgacaagg agctgggcgc cctgctgaag      480

ggcgtggccg gcaacgccca cgccctgcag accggcgact tccggacccc cgccgagctg      540

gccctgaaca agttcgagaa ggagagcggc cacatccgga accagcggag cgactacagc      600

cacaccttca gccggaagga cctgcaggcc gagctgatcc tgctgttcga gaagcagaag      660

gagttcggca acccccacgt gagcggcggc ctgaaggagg gcatcgagac cctgctgatg      720

acccagcggc ccgccctgag cggcgacgcc gtgcagaaga tgctgggcca ctgcaccttc      780

gagcccgccg agcccaaggc cgccaagaac acctacaccg ccgagcggtt catctggctg      840

accaagctga acaacctgcg gatcctggag cagggcagcg agcggcccct gaccgacacc      900

gagcgggcca ccctgatgga cgagccctac cggaagagca agctgaccta cgcccaggcc      960

cggaagctgc tgggcctgga ggacaccgcc ttcttcaagg gcctgcggta cggcaaggac     1020

aacgccgagg ccagcaccct gatggagatg aaggcctacc acgccatcag ccgggccctg     1080

gagaaggagg gcctgaagga caagaagagc cccctgaacc tgagccccga gctgcaggac     1140

gagatcggca ccgccttcag cctgttcaag accgacgagg acatcaccgg ccggctgaag     1200

gaccggatcc agcccgagat cctggaggcc ctgctgaagc acatcagctt cgacaagttc     1260

gtgcagatca gcctgaaggc cctgcggcgg atcgtgcccc tgatggagca gggcaagcgg     1320

tacgacgagg cctgcgccga gatctacggc gaccactacg gcaagaagaa caccgaggag     1380

aagatctacc tgccccccat ccccgccgac gagatccgga accccgtggt gctgcgggcc     1440

ctgagccagg cccggaaggt gatcaacggc gtggtgcggc ggtacggcag ccccgcccgg     1500

atccacatcg agaccgcccg ggaggtgggc aagagcttca aggaccggaa ggagatcgag     1560

aagcggcagg aggagaaccg gaaggaccgg gagaaggccg ccgccaagtt ccgggagtac     1620

ttccccaact tcgtgggcga gcccaagagc aaggacatcc tgaagctgcg gctgtacgag     1680

cagcagcacg gcaagtgcct gtacagcggc aaggagatca acctgggccg gctgaacgag     1740

aagggctacg tggagatcga ccacgccctg cccttcagcc ggacctggga cgacagcttc     1800

aacaacaagg tgctggtgct gggcagcgag aaccagaaca agggcaacca gaccccctac     1860

gagtacttca acggcaagga caacagccgg gagtggcagg agttcaaggc ccgggtggag     1920

accagccggt tcccccggag caagaagcag cggatcctgc tgcagaagtt cgacgaggac     1980

ggcttcaagg agcggaacct gaacgacacc cggtacgtga accggttcct gtgccagttc     2040

gtggccgacc ggatgcggct gaccggcaag ggcaagaagc gggtgttcgc cagcaacggc     2100

cagatcacca acctgctgcg gggcttctgg ggcctgcgga aggtgcgggc cgagaacgac     2160

cggcaccacg ccctggacgc cgtggtggtg gcctgcagca ccgtggccat gcagcagaag     2220

atcacccggt tcgtgcggta caaggagatg aacgccttcg acggcaagac catcgacaag     2280

gagaccggcg aggtgctgca ccagaagacc cacttccccc agccctggga gttcttcgcc     2340

caggaggtga tgatccgggt gttcggcaag cccgacggca agcccgagtt cgaggaggcc     2400

gacaccctgg agaagctgcg gaccctgctg gccgagaagc tgagcagccg gcccgaggcc     2460

gtgcacgagt acgtgacccc cctgttcgtg agccgggccc ccaaccggaa gatgagcggc     2520

cagggccaca tggagaccgt gaagagcgcc aagcggctgg acgagggcgt gagcgtgctg     2580

cgggtgcccc tgacccagct gaagctgaag gacctggaga agatggtgaa ccgggagcgg     2640

gagcccaagc tgtacgaggc cctgaaggcc cggctggagg cccacaagga cgaccccgcc     2700

aaggccttcg ccgagccctt ctacaagtac gacaaggccg gcaaccggac ccagcaggtg     2760

aaggccgtgc gggtggagca ggtgcagaag accggcgtgt gggtgcggaa ccacaacggc     2820

atcgccgaca acgccaccat ggtgcgggtg gacgtgttcg agaagggcga caagtactac     2880

ctggtgccca tctacagctg gcaggtggcc aagggcatcc tgcccgaccg ggccgtggtg     2940

cagggcaagg acgaggagga ctggcagctg atcgacgaca gcttcaactt caagttcagc     3000

ctgcacccca acgacctggt ggaggtgatc accaagaagg cccggatgtt cggctacttc     3060

gccagctgcc accggggcac cggcaacatc aacatccgga tccacgacct ggaccacaag     3120

atcggcaaga acggcatcct ggagggcatc ggcgtgaaga ccgccctgag cttccagaag     3180

taccagatcg acgagctggg caaggagatc cggccctgcc ggctgaagaa gcggcccccc     3240

gtgcggagcg gcaagcggac cgccgacggc agcgagttcg agagccccaa gaagaagcgg     3300

aaggtggagt ga                                                         3312


<210>  330

<400>  330
000

<210>  331

<400>  331
000

<210>  332

<400>  332
000

<210>  333

<400>  333
000

<210>  334

<400>  334
000

<210>  335

<400>  335
000

<210>  336

<400>  336
000

<210>  337

<400>  337
000

<210>  338

<400>  338
000

<210>  339

<400>  339
000

<210>  340

<400>  340
000

<210>  341

<400>  341
000

<210>  342

<400>  342
000

<210>  343

<400>  343
000

<210>  344

<400>  344
000

<210>  345

<400>  345
000

<210>  346
<211>  4134
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 ORF using low A codons of Table 5 (no start or 
       stop codons; suitable for inclusion in fusion protein coding 
       sequence)

<400>  346
gacaagaagt actccatcgg cctggacatc ggcaccaact ccgtgggctg ggccgtgatc       60

accgacgagt acaaggtgcc ctccaagaag ttcaaggtgc tgggcaacac cgaccggcac      120

tccatcaaga agaacctgat cggcgccctg ctgttcgact ccggcgagac cgccgaggcc      180

acccggctga agcggaccgc ccggcggcgg tacacccggc ggaagaaccg gatctgctac      240

ctgcaggaga tcttctccaa cgagatggcc aaggtggacg actccttctt ccaccggctg      300

gaggagtcct tcctggtgga ggaggacaag aagcacgagc ggcaccccat cttcggcaac      360

atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gcggaagaag      420

ctggtggact ccaccgacaa ggccgacctg cggctgatct acctggccct ggcccacatg      480

atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa ctccgacgtg      540

gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga gaaccccatc      600

aacgcctccg gcgtggacgc caaggccatc ctgtccgccc ggctgtccaa gtcccggcgg      660

ctggagaacc tgatcgccca gctgcccggc gagaagaaga acggcctgtt cggcaacctg      720

atcgccctgt ccctgggcct gacccccaac ttcaagtcca acttcgacct ggccgaggac      780

gccaagctgc agctgtccaa ggacacctac gacgacgacc tggacaacct gctggcccag      840

atcggcgacc agtacgccga cctgttcctg gccgccaaga acctgtccga cgccatcctg      900

ctgtccgaca tcctgcgggt gaacaccgag atcaccaagg cccccctgtc cgcctccatg      960

atcaagcggt acgacgagca ccaccaggac ctgaccctgc tgaaggccct ggtgcggcag     1020

cagctgcccg agaagtacaa ggagatcttc ttcgaccagt ccaagaacgg ctacgccggc     1080

tacatcgacg gcggcgcctc ccaggaggag ttctacaagt tcatcaagcc catcctggag     1140

aagatggacg gcaccgagga gctgctggtg aagctgaacc gggaggacct gctgcggaag     1200

cagcggacct tcgacaacgg ctccatcccc caccagatcc acctgggcga gctgcacgcc     1260

atcctgcggc ggcaggagga cttctacccc ttcctgaagg acaaccggga gaagatcgag     1320

aagatcctga ccttccggat cccctactac gtgggccccc tggcccgggg caactcccgg     1380

ttcgcctgga tgacccggaa gtccgaggag accatcaccc cctggaactt cgaggaggtg     1440

gtggacaagg gcgcctccgc ccagtccttc atcgagcgga tgaccaactt cgacaagaac     1500

ctgcccaacg agaaggtgct gcccaagcac tccctgctgt acgagtactt caccgtgtac     1560

aacgagctga ccaaggtgaa gtacgtgacc gagggcatgc ggaagcccgc cttcctgtcc     1620

ggcgagcaga agaaggccat cgtggacctg ctgttcaaga ccaaccggaa ggtgaccgtg     1680

aagcagctga aggaggacta cttcaagaag atcgagtgct tcgactccgt ggagatctcc     1740

ggcgtggagg accggttcaa cgcctccctg ggcacctacc acgacctgct gaagatcatc     1800

aaggacaagg acttcctgga caacgaggag aacgaggaca tcctggagga catcgtgctg     1860

accctgaccc tgttcgagga ccgggagatg atcgaggagc ggctgaagac ctacgcccac     1920

ctgttcgacg acaaggtgat gaagcagctg aagcggcggc ggtacaccgg ctggggccgg     1980

ctgtcccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac catcctggac     2040

ttcctgaagt ccgacggctt cgccaaccgg aacttcatgc agctgatcca cgacgactcc     2100

ctgaccttca aggaggacat ccagaaggcc caggtgtccg gccagggcga ctccctgcac     2160

gagcacatcg ccaacctggc cggctccccc gccatcaaga agggcatcct gcagaccgtg     2220

aaggtggtgg acgagctggt gaaggtgatg ggccggcaca agcccgagaa catcgtgatc     2280

gagatggccc gggagaacca gaccacccag aagggccaga agaactcccg ggagcggatg     2340

aagcggatcg aggagggcat caaggagctg ggctcccaga tcctgaagga gcaccccgtg     2400

gagaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa cggccgggac     2460

atgtacgtgg accaggagct ggacatcaac cggctgtccg actacgacgt ggaccacatc     2520

gtgccccagt ccttcctgaa ggacgactcc atcgacaaca aggtgctgac ccggtccgac     2580

aagaaccggg gcaagtccga caacgtgccc tccgaggagg tggtgaagaa gatgaagaac     2640

tactggcggc agctgctgaa cgccaagctg atcacccagc ggaagttcga caacctgacc     2700

aaggccgagc ggggcggcct gtccgagctg gacaaggccg gcttcatcaa gcggcagctg     2760

gtggagaccc ggcagatcac caagcacgtg gcccagatcc tggactcccg gatgaacacc     2820

aagtacgacg agaacgacaa gctgatccgg gaggtgaagg tgatcaccct gaagtccaag     2880

ctggtgtccg acttccggaa ggacttccag ttctacaagg tgcgggagat caacaactac     2940

caccacgccc acgacgccta cctgaacgcc gtggtgggca ccgccctgat caagaagtac     3000

cccaagctgg agtccgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg     3060

atcgccaagt ccgagcagga gatcggcaag gccaccgcca agtacttctt ctactccaac     3120

atcatgaact tcttcaagac cgagatcacc ctggccaacg gcgagatccg gaagcggccc     3180

ctgatcgaga ccaacggcga gaccggcgag atcgtgtggg acaagggccg ggacttcgcc     3240

accgtgcgga aggtgctgtc catgccccag gtgaacatcg tgaagaagac cgaggtgcag     3300

accggcggct tctccaagga gtccatcctg cccaagcgga actccgacaa gctgatcgcc     3360

cggaagaagg actgggaccc caagaagtac ggcggcttcg actcccccac cgtggcctac     3420

tccgtgctgg tggtggccaa ggtggagaag ggcaagtcca agaagctgaa gtccgtgaag     3480

gagctgctgg gcatcaccat catggagcgg tcctccttcg agaagaaccc catcgacttc     3540

ctggaggcca agggctacaa ggaggtgaag aaggacctga tcatcaagct gcccaagtac     3600

tccctgttcg agctggagaa cggccggaag cggatgctgg cctccgccgg cgagctgcag     3660

aagggcaacg agctggccct gccctccaag tacgtgaact tcctgtacct ggcctcccac     3720

tacgagaagc tgaagggctc ccccgaggac aacgagcaga agcagctgtt cgtggagcag     3780

cacaagcact acctggacga gatcatcgag cagatctccg agttctccaa gcgggtgatc     3840

ctggccgacg ccaacctgga caaggtgctg tccgcctaca acaagcaccg ggacaagccc     3900

atccgggagc aggccgagaa catcatccac ctgttcaccc tgaccaacct gggcgccccc     3960

gccgccttca agtacttcga caccaccatc gaccggaagc ggtacacctc caccaaggag     4020

gtgctggacg ccaccctgat ccaccagtcc atcaccggcc tgtacgagac ccggatcgac     4080

ctgtcccagc tgggcggcga cggcggcggc tcccccaaga agaagcggaa ggtg           4134


<210>  347
<211>  4134
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 ORF using low A/U codons of Table 5 (no start or 
       stop codons; suitable for inclusion in fusion protein coding 
       sequence)

<400>  347
gacaagaagt acagcatcgg cctggacatc ggcaccaaca gcgtgggctg ggccgtgatc       60

accgacgagt acaaggtgcc cagcaagaag ttcaaggtgc tgggcaacac cgaccggcac      120

agcatcaaga agaacctgat cggcgccctg ctgttcgaca gcggcgagac cgccgaggcc      180

acccggctga agcggaccgc ccggcggcgg tacacccggc ggaagaaccg gatctgctac      240

ctgcaggaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccaccggctg      300

gaggagagct tcctggtgga ggaggacaag aagcacgagc ggcaccccat cttcggcaac      360

atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gcggaagaag      420

ctggtggaca gcaccgacaa ggccgacctg cggctgatct acctggccct ggcccacatg      480

atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg      540

gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga gaaccccatc      600

aacgccagcg gcgtggacgc caaggccatc ctgagcgccc ggctgagcaa gagccggcgg      660

ctggagaacc tgatcgccca gctgcccggc gagaagaaga acggcctgtt cggcaacctg      720

atcgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggac      780

gccaagctgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag      840

atcggcgacc agtacgccga cctgttcctg gccgccaaga acctgagcga cgccatcctg      900

ctgagcgaca tcctgcgggt gaacaccgag atcaccaagg cccccctgag cgccagcatg      960

atcaagcggt acgacgagca ccaccaggac ctgaccctgc tgaaggccct ggtgcggcag     1020

cagctgcccg agaagtacaa ggagatcttc ttcgaccaga gcaagaacgg ctacgccggc     1080

tacatcgacg gcggcgccag ccaggaggag ttctacaagt tcatcaagcc catcctggag     1140

aagatggacg gcaccgagga gctgctggtg aagctgaacc gggaggacct gctgcggaag     1200

cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggcga gctgcacgcc     1260

atcctgcggc ggcaggagga cttctacccc ttcctgaagg acaaccggga gaagatcgag     1320

aagatcctga ccttccggat cccctactac gtgggccccc tggcccgggg caacagccgg     1380

ttcgcctgga tgacccggaa gagcgaggag accatcaccc cctggaactt cgaggaggtg     1440

gtggacaagg gcgccagcgc ccagagcttc atcgagcgga tgaccaactt cgacaagaac     1500

ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtac     1560

aacgagctga ccaaggtgaa gtacgtgacc gagggcatgc ggaagcccgc cttcctgagc     1620

ggcgagcaga agaaggccat cgtggacctg ctgttcaaga ccaaccggaa ggtgaccgtg     1680

aagcagctga aggaggacta cttcaagaag atcgagtgct tcgacagcgt ggagatcagc     1740

ggcgtggagg accggttcaa cgccagcctg ggcacctacc acgacctgct gaagatcatc     1800

aaggacaagg acttcctgga caacgaggag aacgaggaca tcctggagga catcgtgctg     1860

accctgaccc tgttcgagga ccgggagatg atcgaggagc ggctgaagac ctacgcccac     1920

ctgttcgacg acaaggtgat gaagcagctg aagcggcggc ggtacaccgg ctggggccgg     1980

ctgagccgga agctgatcaa cggcatccgg gacaagcaga gcggcaagac catcctggac     2040

ttcctgaaga gcgacggctt cgccaaccgg aacttcatgc agctgatcca cgacgacagc     2100

ctgaccttca aggaggacat ccagaaggcc caggtgagcg gccagggcga cagcctgcac     2160

gagcacatcg ccaacctggc cggcagcccc gccatcaaga agggcatcct gcagaccgtg     2220

aaggtggtgg acgagctggt gaaggtgatg ggccggcaca agcccgagaa catcgtgatc     2280

gagatggccc gggagaacca gaccacccag aagggccaga agaacagccg ggagcggatg     2340

aagcggatcg aggagggcat caaggagctg ggcagccaga tcctgaagga gcaccccgtg     2400

gagaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa cggccgggac     2460

atgtacgtgg accaggagct ggacatcaac cggctgagcg actacgacgt ggaccacatc     2520

gtgccccaga gcttcctgaa ggacgacagc atcgacaaca aggtgctgac ccggagcgac     2580

aagaaccggg gcaagagcga caacgtgccc agcgaggagg tggtgaagaa gatgaagaac     2640

tactggcggc agctgctgaa cgccaagctg atcacccagc ggaagttcga caacctgacc     2700

aaggccgagc ggggcggcct gagcgagctg gacaaggccg gcttcatcaa gcggcagctg     2760

gtggagaccc ggcagatcac caagcacgtg gcccagatcc tggacagccg gatgaacacc     2820

aagtacgacg agaacgacaa gctgatccgg gaggtgaagg tgatcaccct gaagagcaag     2880

ctggtgagcg acttccggaa ggacttccag ttctacaagg tgcgggagat caacaactac     2940

caccacgccc acgacgccta cctgaacgcc gtggtgggca ccgccctgat caagaagtac     3000

cccaagctgg agagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg     3060

atcgccaaga gcgagcagga gatcggcaag gccaccgcca agtacttctt ctacagcaac     3120

atcatgaact tcttcaagac cgagatcacc ctggccaacg gcgagatccg gaagcggccc     3180

ctgatcgaga ccaacggcga gaccggcgag atcgtgtggg acaagggccg ggacttcgcc     3240

accgtgcgga aggtgctgag catgccccag gtgaacatcg tgaagaagac cgaggtgcag     3300

accggcggct tcagcaagga gagcatcctg cccaagcgga acagcgacaa gctgatcgcc     3360

cggaagaagg actgggaccc caagaagtac ggcggcttcg acagccccac cgtggcctac     3420

agcgtgctgg tggtggccaa ggtggagaag ggcaagagca agaagctgaa gagcgtgaag     3480

gagctgctgg gcatcaccat catggagcgg agcagcttcg agaagaaccc catcgacttc     3540

ctggaggcca agggctacaa ggaggtgaag aaggacctga tcatcaagct gcccaagtac     3600

agcctgttcg agctggagaa cggccggaag cggatgctgg ccagcgccgg cgagctgcag     3660

aagggcaacg agctggccct gcccagcaag tacgtgaact tcctgtacct ggccagccac     3720

tacgagaagc tgaagggcag ccccgaggac aacgagcaga agcagctgtt cgtggagcag     3780

cacaagcact acctggacga gatcatcgag cagatcagcg agttcagcaa gcgggtgatc     3840

ctggccgacg ccaacctgga caaggtgctg agcgcctaca acaagcaccg ggacaagccc     3900

atccgggagc aggccgagaa catcatccac ctgttcaccc tgaccaacct gggcgccccc     3960

gccgccttca agtacttcga caccaccatc gaccggaagc ggtacaccag caccaaggag     4020

gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac ccggatcgac     4080

ctgagccagc tgggcggcga cggcggcggc agccccaaga agaagcggaa ggtg           4134


<210>  348
<211>  4173
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 ORF using low A codons of Table 5, with two 
       C-terminal NLS sequences (no start or stop codons; suitable for 
       inclusion in fusion protein coding sequence)

<400>  348
gacaagaagt actccatcgg cctggacatc ggcaccaact ccgtgggctg ggccgtgatc       60

accgacgagt acaaggtgcc ctccaagaag ttcaaggtgc tgggcaacac cgaccggcac      120

tccatcaaga agaacctgat cggcgccctg ctgttcgact ccggcgagac cgccgaggcc      180

acccggctga agcggaccgc ccggcggcgg tacacccggc ggaagaaccg gatctgctac      240

ctgcaggaga tcttctccaa cgagatggcc aaggtggacg actccttctt ccaccggctg      300

gaggagtcct tcctggtgga ggaggacaag aagcacgagc ggcaccccat cttcggcaac      360

atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gcggaagaag      420

ctggtggact ccaccgacaa ggccgacctg cggctgatct acctggccct ggcccacatg      480

atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa ctccgacgtg      540

gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga gaaccccatc      600

aacgcctccg gcgtggacgc caaggccatc ctgtccgccc ggctgtccaa gtcccggcgg      660

ctggagaacc tgatcgccca gctgcccggc gagaagaaga acggcctgtt cggcaacctg      720

atcgccctgt ccctgggcct gacccccaac ttcaagtcca acttcgacct ggccgaggac      780

gccaagctgc agctgtccaa ggacacctac gacgacgacc tggacaacct gctggcccag      840

atcggcgacc agtacgccga cctgttcctg gccgccaaga acctgtccga cgccatcctg      900

ctgtccgaca tcctgcgggt gaacaccgag atcaccaagg cccccctgtc cgcctccatg      960

atcaagcggt acgacgagca ccaccaggac ctgaccctgc tgaaggccct ggtgcggcag     1020

cagctgcccg agaagtacaa ggagatcttc ttcgaccagt ccaagaacgg ctacgccggc     1080

tacatcgacg gcggcgcctc ccaggaggag ttctacaagt tcatcaagcc catcctggag     1140

aagatggacg gcaccgagga gctgctggtg aagctgaacc gggaggacct gctgcggaag     1200

cagcggacct tcgacaacgg ctccatcccc caccagatcc acctgggcga gctgcacgcc     1260

atcctgcggc ggcaggagga cttctacccc ttcctgaagg acaaccggga gaagatcgag     1320

aagatcctga ccttccggat cccctactac gtgggccccc tggcccgggg caactcccgg     1380

ttcgcctgga tgacccggaa gtccgaggag accatcaccc cctggaactt cgaggaggtg     1440

gtggacaagg gcgcctccgc ccagtccttc atcgagcgga tgaccaactt cgacaagaac     1500

ctgcccaacg agaaggtgct gcccaagcac tccctgctgt acgagtactt caccgtgtac     1560

aacgagctga ccaaggtgaa gtacgtgacc gagggcatgc ggaagcccgc cttcctgtcc     1620

ggcgagcaga agaaggccat cgtggacctg ctgttcaaga ccaaccggaa ggtgaccgtg     1680

aagcagctga aggaggacta cttcaagaag atcgagtgct tcgactccgt ggagatctcc     1740

ggcgtggagg accggttcaa cgcctccctg ggcacctacc acgacctgct gaagatcatc     1800

aaggacaagg acttcctgga caacgaggag aacgaggaca tcctggagga catcgtgctg     1860

accctgaccc tgttcgagga ccgggagatg atcgaggagc ggctgaagac ctacgcccac     1920

ctgttcgacg acaaggtgat gaagcagctg aagcggcggc ggtacaccgg ctggggccgg     1980

ctgtcccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac catcctggac     2040

ttcctgaagt ccgacggctt cgccaaccgg aacttcatgc agctgatcca cgacgactcc     2100

ctgaccttca aggaggacat ccagaaggcc caggtgtccg gccagggcga ctccctgcac     2160

gagcacatcg ccaacctggc cggctccccc gccatcaaga agggcatcct gcagaccgtg     2220

aaggtggtgg acgagctggt gaaggtgatg ggccggcaca agcccgagaa catcgtgatc     2280

gagatggccc gggagaacca gaccacccag aagggccaga agaactcccg ggagcggatg     2340

aagcggatcg aggagggcat caaggagctg ggctcccaga tcctgaagga gcaccccgtg     2400

gagaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa cggccgggac     2460

atgtacgtgg accaggagct ggacatcaac cggctgtccg actacgacgt ggaccacatc     2520

gtgccccagt ccttcctgaa ggacgactcc atcgacaaca aggtgctgac ccggtccgac     2580

aagaaccggg gcaagtccga caacgtgccc tccgaggagg tggtgaagaa gatgaagaac     2640

tactggcggc agctgctgaa cgccaagctg atcacccagc ggaagttcga caacctgacc     2700

aaggccgagc ggggcggcct gtccgagctg gacaaggccg gcttcatcaa gcggcagctg     2760

gtggagaccc ggcagatcac caagcacgtg gcccagatcc tggactcccg gatgaacacc     2820

aagtacgacg agaacgacaa gctgatccgg gaggtgaagg tgatcaccct gaagtccaag     2880

ctggtgtccg acttccggaa ggacttccag ttctacaagg tgcgggagat caacaactac     2940

caccacgccc acgacgccta cctgaacgcc gtggtgggca ccgccctgat caagaagtac     3000

cccaagctgg agtccgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg     3060

atcgccaagt ccgagcagga gatcggcaag gccaccgcca agtacttctt ctactccaac     3120

atcatgaact tcttcaagac cgagatcacc ctggccaacg gcgagatccg gaagcggccc     3180

ctgatcgaga ccaacggcga gaccggcgag atcgtgtggg acaagggccg ggacttcgcc     3240

accgtgcgga aggtgctgtc catgccccag gtgaacatcg tgaagaagac cgaggtgcag     3300

accggcggct tctccaagga gtccatcctg cccaagcgga actccgacaa gctgatcgcc     3360

cggaagaagg actgggaccc caagaagtac ggcggcttcg actcccccac cgtggcctac     3420

tccgtgctgg tggtggccaa ggtggagaag ggcaagtcca agaagctgaa gtccgtgaag     3480

gagctgctgg gcatcaccat catggagcgg tcctccttcg agaagaaccc catcgacttc     3540

ctggaggcca agggctacaa ggaggtgaag aaggacctga tcatcaagct gcccaagtac     3600

tccctgttcg agctggagaa cggccggaag cggatgctgg cctccgccgg cgagctgcag     3660

aagggcaacg agctggccct gccctccaag tacgtgaact tcctgtacct ggcctcccac     3720

tacgagaagc tgaagggctc ccccgaggac aacgagcaga agcagctgtt cgtggagcag     3780

cacaagcact acctggacga gatcatcgag cagatctccg agttctccaa gcgggtgatc     3840

ctggccgacg ccaacctgga caaggtgctg tccgcctaca acaagcaccg ggacaagccc     3900

atccgggagc aggccgagaa catcatccac ctgttcaccc tgaccaacct gggcgccccc     3960

gccgccttca agtacttcga caccaccatc gaccggaagc ggtacacctc caccaaggag     4020

gtgctggacg ccaccctgat ccaccagtcc atcaccggcc tgtacgagac ccggatcgac     4080

ctgtcccagc tgggcggcga cggctccggc tcccccaaga agaagcggaa ggtggacggc     4140

tcccccaaga agaagcggaa ggtggactcc ggc                                  4173


<210>  349

<400>  349
000

<210>  350

<400>  350
000

<210>  351

<400>  351
000

<210>  352

<400>  352
000

<210>  353

<400>  353
000

<210>  354

<400>  354
000

<210>  355
<211>  4173
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 ORF using low A/U codons of Table 4, with two 
       C-terminal NLS sequences (no start or stop codons; suitable for 
       inclusion in fusion protein coding sequence)

<400>  355
gacaagaagt acagcatcgg cctggacatc ggcaccaaca gcgtgggctg ggccgtgatc       60

accgacgagt acaaggtgcc cagcaagaag ttcaaggtgc tgggcaacac cgaccggcac      120

agcatcaaga agaacctgat cggcgccctg ctgttcgaca gcggcgagac cgccgaggcc      180

acccggctga agcggaccgc ccggcggcgg tacacccggc ggaagaaccg gatctgctac      240

ctgcaggaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccaccggctg      300

gaggagagct tcctggtgga ggaggacaag aagcacgagc ggcaccccat cttcggcaac      360

atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gcggaagaag      420

ctggtggaca gcaccgacaa ggccgacctg cggctgatct acctggccct ggcccacatg      480

atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg      540

gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga gaaccccatc      600

aacgccagcg gcgtggacgc caaggccatc ctgagcgccc ggctgagcaa gagccggcgg      660

ctggagaacc tgatcgccca gctgcccggc gagaagaaga acggcctgtt cggcaacctg      720

atcgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggac      780

gccaagctgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag      840

atcggcgacc agtacgccga cctgttcctg gccgccaaga acctgagcga cgccatcctg      900

ctgagcgaca tcctgcgggt gaacaccgag atcaccaagg cccccctgag cgccagcatg      960

atcaagcggt acgacgagca ccaccaggac ctgaccctgc tgaaggccct ggtgcggcag     1020

cagctgcccg agaagtacaa ggagatcttc ttcgaccaga gcaagaacgg ctacgccggc     1080

tacatcgacg gcggcgccag ccaggaggag ttctacaagt tcatcaagcc catcctggag     1140

aagatggacg gcaccgagga gctgctggtg aagctgaacc gggaggacct gctgcggaag     1200

cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggcga gctgcacgcc     1260

atcctgcggc ggcaggagga cttctacccc ttcctgaagg acaaccggga gaagatcgag     1320

aagatcctga ccttccggat cccctactac gtgggccccc tggcccgggg caacagccgg     1380

ttcgcctgga tgacccggaa gagcgaggag accatcaccc cctggaactt cgaggaggtg     1440

gtggacaagg gcgccagcgc ccagagcttc atcgagcgga tgaccaactt cgacaagaac     1500

ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtac     1560

aacgagctga ccaaggtgaa gtacgtgacc gagggcatgc ggaagcccgc cttcctgagc     1620

ggcgagcaga agaaggccat cgtggacctg ctgttcaaga ccaaccggaa ggtgaccgtg     1680

aagcagctga aggaggacta cttcaagaag atcgagtgct tcgacagcgt ggagatcagc     1740

ggcgtggagg accggttcaa cgccagcctg ggcacctacc acgacctgct gaagatcatc     1800

aaggacaagg acttcctgga caacgaggag aacgaggaca tcctggagga catcgtgctg     1860

accctgaccc tgttcgagga ccgggagatg atcgaggagc ggctgaagac ctacgcccac     1920

ctgttcgacg acaaggtgat gaagcagctg aagcggcggc ggtacaccgg ctggggccgg     1980

ctgagccgga agctgatcaa cggcatccgg gacaagcaga gcggcaagac catcctggac     2040

ttcctgaaga gcgacggctt cgccaaccgg aacttcatgc agctgatcca cgacgacagc     2100

ctgaccttca aggaggacat ccagaaggcc caggtgagcg gccagggcga cagcctgcac     2160

gagcacatcg ccaacctggc cggcagcccc gccatcaaga agggcatcct gcagaccgtg     2220

aaggtggtgg acgagctggt gaaggtgatg ggccggcaca agcccgagaa catcgtgatc     2280

gagatggccc gggagaacca gaccacccag aagggccaga agaacagccg ggagcggatg     2340

aagcggatcg aggagggcat caaggagctg ggcagccaga tcctgaagga gcaccccgtg     2400

gagaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa cggccgggac     2460

atgtacgtgg accaggagct ggacatcaac cggctgagcg actacgacgt ggaccacatc     2520

gtgccccaga gcttcctgaa ggacgacagc atcgacaaca aggtgctgac ccggagcgac     2580

aagaaccggg gcaagagcga caacgtgccc agcgaggagg tggtgaagaa gatgaagaac     2640

tactggcggc agctgctgaa cgccaagctg atcacccagc ggaagttcga caacctgacc     2700

aaggccgagc ggggcggcct gagcgagctg gacaaggccg gcttcatcaa gcggcagctg     2760

gtggagaccc ggcagatcac caagcacgtg gcccagatcc tggacagccg gatgaacacc     2820

aagtacgacg agaacgacaa gctgatccgg gaggtgaagg tgatcaccct gaagagcaag     2880

ctggtgagcg acttccggaa ggacttccag ttctacaagg tgcgggagat caacaactac     2940

caccacgccc acgacgccta cctgaacgcc gtggtgggca ccgccctgat caagaagtac     3000

cccaagctgg agagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg     3060

atcgccaaga gcgagcagga gatcggcaag gccaccgcca agtacttctt ctacagcaac     3120

atcatgaact tcttcaagac cgagatcacc ctggccaacg gcgagatccg gaagcggccc     3180

ctgatcgaga ccaacggcga gaccggcgag atcgtgtggg acaagggccg ggacttcgcc     3240

accgtgcgga aggtgctgag catgccccag gtgaacatcg tgaagaagac cgaggtgcag     3300

accggcggct tcagcaagga gagcatcctg cccaagcgga acagcgacaa gctgatcgcc     3360

cggaagaagg actgggaccc caagaagtac ggcggcttcg acagccccac cgtggcctac     3420

agcgtgctgg tggtggccaa ggtggagaag ggcaagagca agaagctgaa gagcgtgaag     3480

gagctgctgg gcatcaccat catggagcgg agcagcttcg agaagaaccc catcgacttc     3540

ctggaggcca agggctacaa ggaggtgaag aaggacctga tcatcaagct gcccaagtac     3600

agcctgttcg agctggagaa cggccggaag cggatgctgg ccagcgccgg cgagctgcag     3660

aagggcaacg agctggccct gcccagcaag tacgtgaact tcctgtacct ggccagccac     3720

tacgagaagc tgaagggcag ccccgaggac aacgagcaga agcagctgtt cgtggagcag     3780

cacaagcact acctggacga gatcatcgag cagatcagcg agttcagcaa gcgggtgatc     3840

ctggccgacg ccaacctgga caaggtgctg agcgcctaca acaagcaccg ggacaagccc     3900

atccgggagc aggccgagaa catcatccac ctgttcaccc tgaccaacct gggcgccccc     3960

gccgccttca agtacttcga caccaccatc gaccggaagc ggtacaccag caccaaggag     4020

gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac ccggatcgac     4080

ctgagccagc tgggcggcga cggcagcggc agccccaaga agaagcggaa ggtggacggc     4140

agccccaaga agaagcggaa ggtggacagc ggc                                  4173


<210>  356
<211>  4101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 ORF using low A/U codons of Table 4 (no NLS and 
       no start or stop codons; suitable for inclusion in fusion protein
       coding sequence)

<400>  356
gacaagaagt acagcatcgg cctggacatc ggcaccaaca gcgtgggctg ggccgtgatc       60

accgacgagt acaaggtgcc cagcaagaag ttcaaggtgc tgggcaacac cgaccggcac      120

agcatcaaga agaacctgat cggcgccctg ctgttcgaca gcggcgagac cgccgaggcc      180

acccggctga agcggaccgc ccggcggcgg tacacccggc ggaagaaccg gatctgctac      240

ctgcaggaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccaccggctg      300

gaggagagct tcctggtgga ggaggacaag aagcacgagc ggcaccccat cttcggcaac      360

atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gcggaagaag      420

ctggtggaca gcaccgacaa ggccgacctg cggctgatct acctggccct ggcccacatg      480

atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg      540

gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga gaaccccatc      600

aacgccagcg gcgtggacgc caaggccatc ctgagcgccc ggctgagcaa gagccggcgg      660

ctggagaacc tgatcgccca gctgcccggc gagaagaaga acggcctgtt cggcaacctg      720

atcgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggac      780

gccaagctgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag      840

atcggcgacc agtacgccga cctgttcctg gccgccaaga acctgagcga cgccatcctg      900

ctgagcgaca tcctgcgggt gaacaccgag atcaccaagg cccccctgag cgccagcatg      960

atcaagcggt acgacgagca ccaccaggac ctgaccctgc tgaaggccct ggtgcggcag     1020

cagctgcccg agaagtacaa ggagatcttc ttcgaccaga gcaagaacgg ctacgccggc     1080

tacatcgacg gcggcgccag ccaggaggag ttctacaagt tcatcaagcc catcctggag     1140

aagatggacg gcaccgagga gctgctggtg aagctgaacc gggaggacct gctgcggaag     1200

cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggcga gctgcacgcc     1260

atcctgcggc ggcaggagga cttctacccc ttcctgaagg acaaccggga gaagatcgag     1320

aagatcctga ccttccggat cccctactac gtgggccccc tggcccgggg caacagccgg     1380

ttcgcctgga tgacccggaa gagcgaggag accatcaccc cctggaactt cgaggaggtg     1440

gtggacaagg gcgccagcgc ccagagcttc atcgagcgga tgaccaactt cgacaagaac     1500

ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtac     1560

aacgagctga ccaaggtgaa gtacgtgacc gagggcatgc ggaagcccgc cttcctgagc     1620

ggcgagcaga agaaggccat cgtggacctg ctgttcaaga ccaaccggaa ggtgaccgtg     1680

aagcagctga aggaggacta cttcaagaag atcgagtgct tcgacagcgt ggagatcagc     1740

ggcgtggagg accggttcaa cgccagcctg ggcacctacc acgacctgct gaagatcatc     1800

aaggacaagg acttcctgga caacgaggag aacgaggaca tcctggagga catcgtgctg     1860

accctgaccc tgttcgagga ccgggagatg atcgaggagc ggctgaagac ctacgcccac     1920

ctgttcgacg acaaggtgat gaagcagctg aagcggcggc ggtacaccgg ctggggccgg     1980

ctgagccgga agctgatcaa cggcatccgg gacaagcaga gcggcaagac catcctggac     2040

ttcctgaaga gcgacggctt cgccaaccgg aacttcatgc agctgatcca cgacgacagc     2100

ctgaccttca aggaggacat ccagaaggcc caggtgagcg gccagggcga cagcctgcac     2160

gagcacatcg ccaacctggc cggcagcccc gccatcaaga agggcatcct gcagaccgtg     2220

aaggtggtgg acgagctggt gaaggtgatg ggccggcaca agcccgagaa catcgtgatc     2280

gagatggccc gggagaacca gaccacccag aagggccaga agaacagccg ggagcggatg     2340

aagcggatcg aggagggcat caaggagctg ggcagccaga tcctgaagga gcaccccgtg     2400

gagaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa cggccgggac     2460

atgtacgtgg accaggagct ggacatcaac cggctgagcg actacgacgt ggaccacatc     2520

gtgccccaga gcttcctgaa ggacgacagc atcgacaaca aggtgctgac ccggagcgac     2580

aagaaccggg gcaagagcga caacgtgccc agcgaggagg tggtgaagaa gatgaagaac     2640

tactggcggc agctgctgaa cgccaagctg atcacccagc ggaagttcga caacctgacc     2700

aaggccgagc ggggcggcct gagcgagctg gacaaggccg gcttcatcaa gcggcagctg     2760

gtggagaccc ggcagatcac caagcacgtg gcccagatcc tggacagccg gatgaacacc     2820

aagtacgacg agaacgacaa gctgatccgg gaggtgaagg tgatcaccct gaagagcaag     2880

ctggtgagcg acttccggaa ggacttccag ttctacaagg tgcgggagat caacaactac     2940

caccacgccc acgacgccta cctgaacgcc gtggtgggca ccgccctgat caagaagtac     3000

cccaagctgg agagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg     3060

atcgccaaga gcgagcagga gatcggcaag gccaccgcca agtacttctt ctacagcaac     3120

atcatgaact tcttcaagac cgagatcacc ctggccaacg gcgagatccg gaagcggccc     3180

ctgatcgaga ccaacggcga gaccggcgag atcgtgtggg acaagggccg ggacttcgcc     3240

accgtgcgga aggtgctgag catgccccag gtgaacatcg tgaagaagac cgaggtgcag     3300

accggcggct tcagcaagga gagcatcctg cccaagcgga acagcgacaa gctgatcgcc     3360

cggaagaagg actgggaccc caagaagtac ggcggcttcg acagccccac cgtggcctac     3420

agcgtgctgg tggtggccaa ggtggagaag ggcaagagca agaagctgaa gagcgtgaag     3480

gagctgctgg gcatcaccat catggagcgg agcagcttcg agaagaaccc catcgacttc     3540

ctggaggcca agggctacaa ggaggtgaag aaggacctga tcatcaagct gcccaagtac     3600

agcctgttcg agctggagaa cggccggaag cggatgctgg ccagcgccgg cgagctgcag     3660

aagggcaacg agctggccct gcccagcaag tacgtgaact tcctgtacct ggccagccac     3720

tacgagaagc tgaagggcag ccccgaggac aacgagcaga agcagctgtt cgtggagcag     3780

cacaagcact acctggacga gatcatcgag cagatcagcg agttcagcaa gcgggtgatc     3840

ctggccgacg ccaacctgga caaggtgctg agcgcctaca acaagcaccg ggacaagccc     3900

atccgggagc aggccgagaa catcatccac ctgttcaccc tgaccaacct gggcgccccc     3960

gccgccttca agtacttcga caccaccatc gaccggaagc ggtacaccag caccaaggag     4020

gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac ccggatcgac     4080

ctgagccagc tgggcggcga c                                               4101


<210>  357

<400>  357
000

<210>  358

<400>  358
000

<210>  359

<400>  359
000

<210>  360

<400>  360
000

<210>  361

<400>  361
000

<210>  362

<400>  362
000

<210>  363
<211>  3306
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nme Cas9 ORF using low A codons of Table 5 (no start 
       or stop codons; suitable for inclusion in fusion protein coding 
       sequence)

<400>  363
gccgccttca agcccaactc catcaactac atcctgggcc tggacatcgg catcgcctcc       60

gtgggctggg ccatggtgga gatcgacgag gaggagaacc ccatccggct gatcgacctg      120

ggcgtgcggg tgttcgagcg ggccgaggtg cccaagaccg gcgactccct ggccatggcc      180

cggcggctgg cccggtccgt gcggcggctg acccggcggc gggcccaccg gctgctgcgg      240

acccggcggc tgctgaagcg ggagggcgtg ctgcaggccg ccaacttcga cgagaacggc      300

ctgatcaagt ccctgcccaa caccccctgg cagctgcggg ccgccgccct ggaccggaag      360

ctgacccccc tggagtggtc cgccgtgctg ctgcacctga tcaagcaccg gggctacctg      420

tcccagcgga agaacgaggg cgagaccgcc gacaaggagc tgggcgccct gctgaagggc      480

gtggccggca acgcccacgc cctgcagacc ggcgacttcc ggacccccgc cgagctggcc      540

ctgaacaagt tcgagaagga gtccggccac atccggaacc agcggtccga ctactcccac      600

accttctccc ggaaggacct gcaggccgag ctgatcctgc tgttcgagaa gcagaaggag      660

ttcggcaacc cccacgtgtc cggcggcctg aaggagggca tcgagaccct gctgatgacc      720

cagcggcccg ccctgtccgg cgacgccgtg cagaagatgc tgggccactg caccttcgag      780

cccgccgagc ccaaggccgc caagaacacc tacaccgccg agcggttcat ctggctgacc      840

aagctgaaca acctgcggat cctggagcag ggctccgagc ggcccctgac cgacaccgag      900

cgggccaccc tgatggacga gccctaccgg aagtccaagc tgacctacgc ccaggcccgg      960

aagctgctgg gcctggagga caccgccttc ttcaagggcc tgcggtacgg caaggacaac     1020

gccgaggcct ccaccctgat ggagatgaag gcctaccacg ccatctcccg ggccctggag     1080

aaggagggcc tgaaggacaa gaagtccccc ctgaacctgt cccccgagct gcaggacgag     1140

atcggcaccg ccttctccct gttcaagacc gacgaggaca tcaccggccg gctgaaggac     1200

cggatccagc ccgagatcct ggaggccctg ctgaagcaca tctccttcga caagttcgtg     1260

cagatctccc tgaaggccct gcggcggatc gtgcccctga tggagcaggg caagcggtac     1320

gacgaggcct gcgccgagat ctacggcgac cactacggca agaagaacac cgaggagaag     1380

atctacctgc cccccatccc cgccgacgag atccggaacc ccgtggtgct gcgggccctg     1440

tcccaggccc ggaaggtgat caacggcgtg gtgcggcggt acggctcccc cgcccggatc     1500

cacatcgaga ccgcccggga ggtgggcaag tccttcaagg accggaagga gatcgagaag     1560

cggcaggagg agaaccggaa ggaccgggag aaggccgccg ccaagttccg ggagtacttc     1620

cccaacttcg tgggcgagcc caagtccaag gacatcctga agctgcggct gtacgagcag     1680

cagcacggca agtgcctgta ctccggcaag gagatcaacc tgggccggct gaacgagaag     1740

ggctacgtgg agatcgacca cgccctgccc ttctcccgga cctgggacga ctccttcaac     1800

aacaaggtgc tggtgctggg ctccgagaac cagaacaagg gcaaccagac cccctacgag     1860

tacttcaacg gcaaggacaa ctcccgggag tggcaggagt tcaaggcccg ggtggagacc     1920

tcccggttcc cccggtccaa gaagcagcgg atcctgctgc agaagttcga cgaggacggc     1980

ttcaaggagc ggaacctgaa cgacacccgg tacgtgaacc ggttcctgtg ccagttcgtg     2040

gccgaccgga tgcggctgac cggcaagggc aagaagcggg tgttcgcctc caacggccag     2100

atcaccaacc tgctgcgggg cttctggggc ctgcggaagg tgcgggccga gaacgaccgg     2160

caccacgccc tggacgccgt ggtggtggcc tgctccaccg tggccatgca gcagaagatc     2220

acccggttcg tgcggtacaa ggagatgaac gccttcgacg gcaagaccat cgacaaggag     2280

accggcgagg tgctgcacca gaagacccac ttcccccagc cctgggagtt cttcgcccag     2340

gaggtgatga tccgggtgtt cggcaagccc gacggcaagc ccgagttcga ggaggccgac     2400

accctggaga agctgcggac cctgctggcc gagaagctgt cctcccggcc cgaggccgtg     2460

cacgagtacg tgacccccct gttcgtgtcc cgggccccca accggaagat gtccggccag     2520

ggccacatgg agaccgtgaa gtccgccaag cggctggacg agggcgtgtc cgtgctgcgg     2580

gtgcccctga cccagctgaa gctgaaggac ctggagaaga tggtgaaccg ggagcgggag     2640

cccaagctgt acgaggccct gaaggcccgg ctggaggccc acaaggacga ccccgccaag     2700

gccttcgccg agcccttcta caagtacgac aaggccggca accggaccca gcaggtgaag     2760

gccgtgcggg tggagcaggt gcagaagacc ggcgtgtggg tgcggaacca caacggcatc     2820

gccgacaacg ccaccatggt gcgggtggac gtgttcgaga agggcgacaa gtactacctg     2880

gtgcccatct actcctggca ggtggccaag ggcatcctgc ccgaccgggc cgtggtgcag     2940

ggcaaggacg aggaggactg gcagctgatc gacgactcct tcaacttcaa gttctccctg     3000

caccccaacg acctggtgga ggtgatcacc aagaaggccc ggatgttcgg ctacttcgcc     3060

tcctgccacc ggggcaccgg caacatcaac atccggatcc acgacctgga ccacaagatc     3120

ggcaagaacg gcatcctgga gggcatcggc gtgaagaccg ccctgtcctt ccagaagtac     3180

cagatcgacg agctgggcaa ggagatccgg ccctgccggc tgaagaagcg gccccccgtg     3240

cggtccggca agcggaccgc cgacggctcc gagttcgagt cccccaagaa gaagcggaag     3300

gtggag                                                                3306


<210>  364
<211>  3306
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nme Cas9 ORF using low A/U codons of Table 5 (no start
       or stop codons; suitable for inclusion in fusion protein coding 
       sequence)

<400>  364
gccgccttca agcccaacag catcaactac atcctgggcc tggacatcgg catcgccagc       60

gtgggctggg ccatggtgga gatcgacgag gaggagaacc ccatccggct gatcgacctg      120

ggcgtgcggg tgttcgagcg ggccgaggtg cccaagaccg gcgacagcct ggccatggcc      180

cggcggctgg cccggagcgt gcggcggctg acccggcggc gggcccaccg gctgctgcgg      240

acccggcggc tgctgaagcg ggagggcgtg ctgcaggccg ccaacttcga cgagaacggc      300

ctgatcaaga gcctgcccaa caccccctgg cagctgcggg ccgccgccct ggaccggaag      360

ctgacccccc tggagtggag cgccgtgctg ctgcacctga tcaagcaccg gggctacctg      420

agccagcgga agaacgaggg cgagaccgcc gacaaggagc tgggcgccct gctgaagggc      480

gtggccggca acgcccacgc cctgcagacc ggcgacttcc ggacccccgc cgagctggcc      540

ctgaacaagt tcgagaagga gagcggccac atccggaacc agcggagcga ctacagccac      600

accttcagcc ggaaggacct gcaggccgag ctgatcctgc tgttcgagaa gcagaaggag      660

ttcggcaacc cccacgtgag cggcggcctg aaggagggca tcgagaccct gctgatgacc      720

cagcggcccg ccctgagcgg cgacgccgtg cagaagatgc tgggccactg caccttcgag      780

cccgccgagc ccaaggccgc caagaacacc tacaccgccg agcggttcat ctggctgacc      840

aagctgaaca acctgcggat cctggagcag ggcagcgagc ggcccctgac cgacaccgag      900

cgggccaccc tgatggacga gccctaccgg aagagcaagc tgacctacgc ccaggcccgg      960

aagctgctgg gcctggagga caccgccttc ttcaagggcc tgcggtacgg caaggacaac     1020

gccgaggcca gcaccctgat ggagatgaag gcctaccacg ccatcagccg ggccctggag     1080

aaggagggcc tgaaggacaa gaagagcccc ctgaacctga gccccgagct gcaggacgag     1140

atcggcaccg ccttcagcct gttcaagacc gacgaggaca tcaccggccg gctgaaggac     1200

cggatccagc ccgagatcct ggaggccctg ctgaagcaca tcagcttcga caagttcgtg     1260

cagatcagcc tgaaggccct gcggcggatc gtgcccctga tggagcaggg caagcggtac     1320

gacgaggcct gcgccgagat ctacggcgac cactacggca agaagaacac cgaggagaag     1380

atctacctgc cccccatccc cgccgacgag atccggaacc ccgtggtgct gcgggccctg     1440

agccaggccc ggaaggtgat caacggcgtg gtgcggcggt acggcagccc cgcccggatc     1500

cacatcgaga ccgcccggga ggtgggcaag agcttcaagg accggaagga gatcgagaag     1560

cggcaggagg agaaccggaa ggaccgggag aaggccgccg ccaagttccg ggagtacttc     1620

cccaacttcg tgggcgagcc caagagcaag gacatcctga agctgcggct gtacgagcag     1680

cagcacggca agtgcctgta cagcggcaag gagatcaacc tgggccggct gaacgagaag     1740

ggctacgtgg agatcgacca cgccctgccc ttcagccgga cctgggacga cagcttcaac     1800

aacaaggtgc tggtgctggg cagcgagaac cagaacaagg gcaaccagac cccctacgag     1860

tacttcaacg gcaaggacaa cagccgggag tggcaggagt tcaaggcccg ggtggagacc     1920

agccggttcc cccggagcaa gaagcagcgg atcctgctgc agaagttcga cgaggacggc     1980

ttcaaggagc ggaacctgaa cgacacccgg tacgtgaacc ggttcctgtg ccagttcgtg     2040

gccgaccgga tgcggctgac cggcaagggc aagaagcggg tgttcgccag caacggccag     2100

atcaccaacc tgctgcgggg cttctggggc ctgcggaagg tgcgggccga gaacgaccgg     2160

caccacgccc tggacgccgt ggtggtggcc tgcagcaccg tggccatgca gcagaagatc     2220

acccggttcg tgcggtacaa ggagatgaac gccttcgacg gcaagaccat cgacaaggag     2280

accggcgagg tgctgcacca gaagacccac ttcccccagc cctgggagtt cttcgcccag     2340

gaggtgatga tccgggtgtt cggcaagccc gacggcaagc ccgagttcga ggaggccgac     2400

accctggaga agctgcggac cctgctggcc gagaagctga gcagccggcc cgaggccgtg     2460

cacgagtacg tgacccccct gttcgtgagc cgggccccca accggaagat gagcggccag     2520

ggccacatgg agaccgtgaa gagcgccaag cggctggacg agggcgtgag cgtgctgcgg     2580

gtgcccctga cccagctgaa gctgaaggac ctggagaaga tggtgaaccg ggagcgggag     2640

cccaagctgt acgaggccct gaaggcccgg ctggaggccc acaaggacga ccccgccaag     2700

gccttcgccg agcccttcta caagtacgac aaggccggca accggaccca gcaggtgaag     2760

gccgtgcggg tggagcaggt gcagaagacc ggcgtgtggg tgcggaacca caacggcatc     2820

gccgacaacg ccaccatggt gcgggtggac gtgttcgaga agggcgacaa gtactacctg     2880

gtgcccatct acagctggca ggtggccaag ggcatcctgc ccgaccgggc cgtggtgcag     2940

ggcaaggacg aggaggactg gcagctgatc gacgacagct tcaacttcaa gttcagcctg     3000

caccccaacg acctggtgga ggtgatcacc aagaaggccc ggatgttcgg ctacttcgcc     3060

agctgccacc ggggcaccgg caacatcaac atccggatcc acgacctgga ccacaagatc     3120

ggcaagaacg gcatcctgga gggcatcggc gtgaagaccg ccctgagctt ccagaagtac     3180

cagatcgacg agctgggcaa ggagatccgg ccctgccggc tgaagaagcg gccccccgtg     3240

cggagcggca agcggaccgc cgacggcagc gagttcgaga gccccaagaa gaagcggaag     3300

gtggag                                                                3306


<210>  365

<400>  365
000

<210>  366

<400>  366
000

<210>  367

<400>  367
000

<210>  368

<400>  368
000

<210>  369

<400>  369
000

<210>  370

<400>  370
000

<210>  371

<400>  371
000

<210>  372

<400>  372
000

<210>  373

<400>  373
000

<210>  374

<400>  374
000

<210>  375

<400>  375
000

<210>  376
<211>  4405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mRNA transcript with XBG UTRs and Cas9 ORF

<400>  376
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguaca       60

gcaucggccu ggacaucggc accaacagcg ugggcugggc cgugaucacc gacgaguaca      120

aggugcccag caagaaguuc aaggugcugg gcaacaccga cagacacagc aucaagaaga      180

accugaucgg cgcccugcug uucgacagcg gcgagaccgc cgaggccacc agacugaaga      240

gaaccgccag aagaagauac accagaagaa agaacagaau cugcuaccug caggagaucu      300

ucagcaacga gauggccaag guggacgaca gcuucuucca cagacuggag gagagcuucc      360

ugguggagga ggacaagaag cacgagagac accccaucuu cggcaacauc guggacgagg      420

uggccuacca cgagaaguac cccaccaucu accaccugag aaagaagcug guggacagca      480

ccgacaaggc cgaccugaga cugaucuacc uggcccuggc ccacaugauc aaguucagag      540

gccacuuccu gaucgagggc gaccugaacc ccgacaacag cgacguggac aagcuguuca      600

uccagcuggu gcagaccuac aaccagcugu ucgaggagaa ccccaucaac gccagcggcg      660

uggacgccaa ggccauccug agcgccagac ugagcaagag cagaagacug gagaaccuga      720

ucgcccagcu gcccggcgag aagaagaacg gccuguucgg caaccugauc gcccugagcc      780

ugggccugac ccccaacuuc aagagcaacu ucgaccuggc cgaggacgcc aagcugcagc      840

ugagcaagga caccuacgac gacgaccugg acaaccugcu ggcccagauc ggcgaccagu      900

acgccgaccu guuccuggcc gccaagaacc ugagcgacgc cauccugcug agcgacaucc      960

ugagagugaa caccgagauc accaaggccc cccugagcgc cagcaugauc aagagauacg     1020

acgagcacca ccaggaccug acccugcuga aggcccuggu gagacagcag cugcccgaga     1080

aguacaagga gaucuucuuc gaccagagca agaacggcua cgccggcuac aucgacggcg     1140

gcgccagcca ggaggaguuc uacaaguuca ucaagcccau ccuggagaag auggacggca     1200

ccgaggagcu gcuggugaag cugaacagag aggaccugcu gagaaagcag agaaccuucg     1260

acaacggcag caucccccac cagauccacc ugggcgagcu gcacgccauc cugagaagac     1320

aggaggacuu cuaccccuuc cugaaggaca acagagagaa gaucgagaag auccugaccu     1380

ucagaauccc cuacuacgug ggcccccugg ccagaggcaa cagcagauuc gccuggauga     1440

ccagaaagag cgaggagacc aucacccccu ggaacuucga ggagguggug gacaagggcg     1500

ccagcgccca gagcuucauc gagagaauga ccaacuucga caagaaccug cccaacgaga     1560

aggugcugcc caagcacagc cugcuguacg aguacuucac cguguacaac gagcugacca     1620

aggugaagua cgugaccgag ggcaugagaa agcccgccuu ccugagcggc gagcagaaga     1680

aggccaucgu ggaccugcug uucaagacca acagaaaggu gaccgugaag cagcugaagg     1740

aggacuacuu caagaagauc gagugcuucg acagcgugga gaucagcggc guggaggaca     1800

gauucaacgc cagccugggc accuaccacg accugcugaa gaucaucaag gacaaggacu     1860

uccuggacaa cgaggagaac gaggacaucc uggaggacau cgugcugacc cugacccugu     1920

ucgaggacag agagaugauc gaggagagac ugaagaccua cgcccaccug uucgacgaca     1980

aggugaugaa gcagcugaag agaagaagau acaccggcug gggcagacug agcagaaagc     2040

ugaucaacgg caucagagac aagcagagcg gcaagaccau ccuggacuuc cugaagagcg     2100

acggcuucgc caacagaaac uucaugcagc ugauccacga cgacagccug accuucaagg     2160

aggacaucca gaaggcccag gugagcggcc agggcgacag ccugcacgag cacaucgcca     2220

accuggccgg cagccccgcc aucaagaagg gcauccugca gaccgugaag gugguggacg     2280

agcuggugaa ggugaugggc agacacaagc ccgagaacau cgugaucgag auggccagag     2340

agaaccagac cacccagaag ggccagaaga acagcagaga gagaaugaag agaaucgagg     2400

agggcaucaa ggagcugggc agccagaucc ugaaggagca ccccguggag aacacccagc     2460

ugcagaacga gaagcuguac cuguacuacc ugcagaacgg cagagacaug uacguggacc     2520

aggagcugga caucaacaga cugagcgacu acgacgugga ccacaucgug ccccagagcu     2580

uccugaagga cgacagcauc gacaacaagg ugcugaccag aagcgacaag aacagaggca     2640

agagcgacaa cgugcccagc gaggaggugg ugaagaagau gaagaacuac uggagacagc     2700

ugcugaacgc caagcugauc acccagagaa aguucgacaa ccugaccaag gccgagagag     2760

gcggccugag cgagcuggac aaggccggcu ucaucaagag acagcuggug gagaccagac     2820

agaucaccaa gcacguggcc cagauccugg acagcagaau gaacaccaag uacgacgaga     2880

acgacaagcu gaucagagag gugaagguga ucacccugaa gagcaagcug gugagcgacu     2940

ucagaaagga cuuccaguuc uacaagguga gagagaucaa caacuaccac cacgcccacg     3000

acgccuaccu gaacgccgug gugggcaccg cccugaucaa gaaguacccc aagcuggaga     3060

gcgaguucgu guacggcgac uacaaggugu acgacgugag aaagaugauc gccaagagcg     3120

agcaggagau cggcaaggcc accgccaagu acuucuucua cagcaacauc augaacuucu     3180

ucaagaccga gaucacccug gccaacggcg agaucagaaa gagaccccug aucgagacca     3240

acggcgagac cggcgagauc gugugggaca agggcagaga cuucgccacc gugagaaagg     3300

ugcugagcau gccccaggug aacaucguga agaagaccga ggugcagacc ggcggcuuca     3360

gcaaggagag cauccugccc aagagaaaca gcgacaagcu gaucgccaga aagaaggacu     3420

gggaccccaa gaaguacggc ggcuucgaca gccccaccgu ggccuacagc gugcuggugg     3480

uggccaaggu ggagaagggc aagagcaaga agcugaagag cgugaaggag cugcugggca     3540

ucaccaucau ggagagaagc agcuucgaga agaaccccau cgacuuccug gaggccaagg     3600

gcuacaagga ggugaagaag gaccugauca ucaagcugcc caaguacagc cuguucgagc     3660

uggagaacgg cagaaagaga augcuggcca gcgccggcga gcugcagaag ggcaacgagc     3720

uggcccugcc cagcaaguac gugaacuucc uguaccuggc cagccacuac gagaagcuga     3780

agggcagccc cgaggacaac gagcagaagc agcuguucgu ggagcagcac aagcacuacc     3840

uggacgagau caucgagcag aucagcgagu ucagcaagag agugauccug gccgacgcca     3900

accuggacaa ggugcugagc gccuacaaca agcacagaga caagcccauc agagagcagg     3960

ccgagaacau cauccaccug uucacccuga ccaaccuggg cgcccccgcc gccuucaagu     4020

acuucgacac caccaucgac agaaagagau acaccagcac caaggaggug cuggacgcca     4080

cccugaucca ccagagcauc accggccugu acgagaccag aaucgaccug agccagcugg     4140

gcggcgacgg cggcggcagc cccaagaaga agagaaaggu gugacuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucuc     4320

ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaa                                           4405


<210>  377
<211>  4405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mRNA transcript with XBG UTRs and Cas9 ORF with low A 
       codons of Table 5

<400>  377
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguacu       60

ccaucggccu ggacaucggc accaacuccg ugggcugggc cgugaucacc gacgaguaca      120

aggugcccuc caagaaguuc aaggugcugg gcaacaccga ccggcacucc aucaagaaga      180

accugaucgg cgcccugcug uucgacuccg gcgagaccgc cgaggccacc cggcugaagc      240

ggaccgcccg gcggcgguac acccggcgga agaaccggau cugcuaccug caggagaucu      300

ucuccaacga gauggccaag guggacgacu ccuucuucca ccggcuggag gaguccuucc      360

ugguggagga ggacaagaag cacgagcggc accccaucuu cggcaacauc guggacgagg      420

uggccuacca cgagaaguac cccaccaucu accaccugcg gaagaagcug guggacucca      480

ccgacaaggc cgaccugcgg cugaucuacc uggcccuggc ccacaugauc aaguuccggg      540

gccacuuccu gaucgagggc gaccugaacc ccgacaacuc cgacguggac aagcuguuca      600

uccagcuggu gcagaccuac aaccagcugu ucgaggagaa ccccaucaac gccuccggcg      660

uggacgccaa ggccauccug uccgcccggc uguccaaguc ccggcggcug gagaaccuga      720

ucgcccagcu gcccggcgag aagaagaacg gccuguucgg caaccugauc gcccuguccc      780

ugggccugac ccccaacuuc aaguccaacu ucgaccuggc cgaggacgcc aagcugcagc      840

uguccaagga caccuacgac gacgaccugg acaaccugcu ggcccagauc ggcgaccagu      900

acgccgaccu guuccuggcc gccaagaacc uguccgacgc cauccugcug uccgacaucc      960

ugcgggugaa caccgagauc accaaggccc cccuguccgc cuccaugauc aagcgguacg     1020

acgagcacca ccaggaccug acccugcuga aggcccuggu gcggcagcag cugcccgaga     1080

aguacaagga gaucuucuuc gaccagucca agaacggcua cgccggcuac aucgacggcg     1140

gcgccuccca ggaggaguuc uacaaguuca ucaagcccau ccuggagaag auggacggca     1200

ccgaggagcu gcuggugaag cugaaccggg aggaccugcu gcggaagcag cggaccuucg     1260

acaacggcuc caucccccac cagauccacc ugggcgagcu gcacgccauc cugcggcggc     1320

aggaggacuu cuaccccuuc cugaaggaca accgggagaa gaucgagaag auccugaccu     1380

uccggauccc cuacuacgug ggcccccugg cccggggcaa cucccgguuc gccuggauga     1440

cccggaaguc cgaggagacc aucacccccu ggaacuucga ggagguggug gacaagggcg     1500

ccuccgccca guccuucauc gagcggauga ccaacuucga caagaaccug cccaacgaga     1560

aggugcugcc caagcacucc cugcuguacg aguacuucac cguguacaac gagcugacca     1620

aggugaagua cgugaccgag ggcaugcgga agcccgccuu ccuguccggc gagcagaaga     1680

aggccaucgu ggaccugcug uucaagacca accggaaggu gaccgugaag cagcugaagg     1740

aggacuacuu caagaagauc gagugcuucg acuccgugga gaucuccggc guggaggacc     1800

gguucaacgc cucccugggc accuaccacg accugcugaa gaucaucaag gacaaggacu     1860

uccuggacaa cgaggagaac gaggacaucc uggaggacau cgugcugacc cugacccugu     1920

ucgaggaccg ggagaugauc gaggagcggc ugaagaccua cgcccaccug uucgacgaca     1980

aggugaugaa gcagcugaag cggcggcggu acaccggcug gggccggcug ucccggaagc     2040

ugaucaacgg cauccgggac aagcaguccg gcaagaccau ccuggacuuc cugaaguccg     2100

acggcuucgc caaccggaac uucaugcagc ugauccacga cgacucccug accuucaagg     2160

aggacaucca gaaggcccag guguccggcc agggcgacuc ccugcacgag cacaucgcca     2220

accuggccgg cucccccgcc aucaagaagg gcauccugca gaccgugaag gugguggacg     2280

agcuggugaa ggugaugggc cggcacaagc ccgagaacau cgugaucgag auggcccggg     2340

agaaccagac cacccagaag ggccagaaga acucccggga gcggaugaag cggaucgagg     2400

agggcaucaa ggagcugggc ucccagaucc ugaaggagca ccccguggag aacacccagc     2460

ugcagaacga gaagcuguac cuguacuacc ugcagaacgg ccgggacaug uacguggacc     2520

aggagcugga caucaaccgg cuguccgacu acgacgugga ccacaucgug ccccaguccu     2580

uccugaagga cgacuccauc gacaacaagg ugcugacccg guccgacaag aaccggggca     2640

aguccgacaa cgugcccucc gaggaggugg ugaagaagau gaagaacuac uggcggcagc     2700

ugcugaacgc caagcugauc acccagcgga aguucgacaa ccugaccaag gccgagcggg     2760

gcggccuguc cgagcuggac aaggccggcu ucaucaagcg gcagcuggug gagacccggc     2820

agaucaccaa gcacguggcc cagauccugg acucccggau gaacaccaag uacgacgaga     2880

acgacaagcu gauccgggag gugaagguga ucacccugaa guccaagcug guguccgacu     2940

uccggaagga cuuccaguuc uacaaggugc gggagaucaa caacuaccac cacgcccacg     3000

acgccuaccu gaacgccgug gugggcaccg cccugaucaa gaaguacccc aagcuggagu     3060

ccgaguucgu guacggcgac uacaaggugu acgacgugcg gaagaugauc gccaaguccg     3120

agcaggagau cggcaaggcc accgccaagu acuucuucua cuccaacauc augaacuucu     3180

ucaagaccga gaucacccug gccaacggcg agauccggaa gcggccccug aucgagacca     3240

acggcgagac cggcgagauc gugugggaca agggccggga cuucgccacc gugcggaagg     3300

ugcuguccau gccccaggug aacaucguga agaagaccga ggugcagacc ggcggcuucu     3360

ccaaggaguc cauccugccc aagcggaacu ccgacaagcu gaucgcccgg aagaaggacu     3420

gggaccccaa gaaguacggc ggcuucgacu cccccaccgu ggccuacucc gugcuggugg     3480

uggccaaggu ggagaagggc aaguccaaga agcugaaguc cgugaaggag cugcugggca     3540

ucaccaucau ggagcggucc uccuucgaga agaaccccau cgacuuccug gaggccaagg     3600

gcuacaagga ggugaagaag gaccugauca ucaagcugcc caaguacucc cuguucgagc     3660

uggagaacgg ccggaagcgg augcuggccu ccgccggcga gcugcagaag ggcaacgagc     3720

uggcccugcc cuccaaguac gugaacuucc uguaccuggc cucccacuac gagaagcuga     3780

agggcucccc cgaggacaac gagcagaagc agcuguucgu ggagcagcac aagcacuacc     3840

uggacgagau caucgagcag aucuccgagu ucuccaagcg ggugauccug gccgacgcca     3900

accuggacaa ggugcugucc gccuacaaca agcaccggga caagcccauc cgggagcagg     3960

ccgagaacau cauccaccug uucacccuga ccaaccuggg cgcccccgcc gccuucaagu     4020

acuucgacac caccaucgac cggaagcggu acaccuccac caaggaggug cuggacgcca     4080

cccugaucca ccaguccauc accggccugu acgagacccg gaucgaccug ucccagcugg     4140

gcggcgacgg cggcggcucc cccaagaaga agcggaaggu gugacuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucuc     4320

ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaa                                           4405


<210>  378
<211>  4405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mRNA transcript with XBG UTRs and Cas9 ORF with low 
       U/A codons of Table 5

<400>  378
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguaca       60

gcaucggccu ggacaucggc accaacagcg ugggcugggc cgugaucacc gacgaguaca      120

aggugcccag caagaaguuc aaggugcugg gcaacaccga ccggcacagc aucaagaaga      180

accugaucgg cgcccugcug uucgacagcg gcgagaccgc cgaggccacc cggcugaagc      240

ggaccgcccg gcggcgguac acccggcgga agaaccggau cugcuaccug caggagaucu      300

ucagcaacga gauggccaag guggacgaca gcuucuucca ccggcuggag gagagcuucc      360

ugguggagga ggacaagaag cacgagcggc accccaucuu cggcaacauc guggacgagg      420

uggccuacca cgagaaguac cccaccaucu accaccugcg gaagaagcug guggacagca      480

ccgacaaggc cgaccugcgg cugaucuacc uggcccuggc ccacaugauc aaguuccggg      540

gccacuuccu gaucgagggc gaccugaacc ccgacaacag cgacguggac aagcuguuca      600

uccagcuggu gcagaccuac aaccagcugu ucgaggagaa ccccaucaac gccagcggcg      660

uggacgccaa ggccauccug agcgcccggc ugagcaagag ccggcggcug gagaaccuga      720

ucgcccagcu gcccggcgag aagaagaacg gccuguucgg caaccugauc gcccugagcc      780

ugggccugac ccccaacuuc aagagcaacu ucgaccuggc cgaggacgcc aagcugcagc      840

ugagcaagga caccuacgac gacgaccugg acaaccugcu ggcccagauc ggcgaccagu      900

acgccgaccu guuccuggcc gccaagaacc ugagcgacgc cauccugcug agcgacaucc      960

ugcgggugaa caccgagauc accaaggccc cccugagcgc cagcaugauc aagcgguacg     1020

acgagcacca ccaggaccug acccugcuga aggcccuggu gcggcagcag cugcccgaga     1080

aguacaagga gaucuucuuc gaccagagca agaacggcua cgccggcuac aucgacggcg     1140

gcgccagcca ggaggaguuc uacaaguuca ucaagcccau ccuggagaag auggacggca     1200

ccgaggagcu gcuggugaag cugaaccggg aggaccugcu gcggaagcag cggaccuucg     1260

acaacggcag caucccccac cagauccacc ugggcgagcu gcacgccauc cugcggcggc     1320

aggaggacuu cuaccccuuc cugaaggaca accgggagaa gaucgagaag auccugaccu     1380

uccggauccc cuacuacgug ggcccccugg cccggggcaa cagccgguuc gccuggauga     1440

cccggaagag cgaggagacc aucacccccu ggaacuucga ggagguggug gacaagggcg     1500

ccagcgccca gagcuucauc gagcggauga ccaacuucga caagaaccug cccaacgaga     1560

aggugcugcc caagcacagc cugcuguacg aguacuucac cguguacaac gagcugacca     1620

aggugaagua cgugaccgag ggcaugcgga agcccgccuu ccugagcggc gagcagaaga     1680

aggccaucgu ggaccugcug uucaagacca accggaaggu gaccgugaag cagcugaagg     1740

aggacuacuu caagaagauc gagugcuucg acagcgugga gaucagcggc guggaggacc     1800

gguucaacgc cagccugggc accuaccacg accugcugaa gaucaucaag gacaaggacu     1860

uccuggacaa cgaggagaac gaggacaucc uggaggacau cgugcugacc cugacccugu     1920

ucgaggaccg ggagaugauc gaggagcggc ugaagaccua cgcccaccug uucgacgaca     1980

aggugaugaa gcagcugaag cggcggcggu acaccggcug gggccggcug agccggaagc     2040

ugaucaacgg cauccgggac aagcagagcg gcaagaccau ccuggacuuc cugaagagcg     2100

acggcuucgc caaccggaac uucaugcagc ugauccacga cgacagccug accuucaagg     2160

aggacaucca gaaggcccag gugagcggcc agggcgacag ccugcacgag cacaucgcca     2220

accuggccgg cagccccgcc aucaagaagg gcauccugca gaccgugaag gugguggacg     2280

agcuggugaa ggugaugggc cggcacaagc ccgagaacau cgugaucgag auggcccggg     2340

agaaccagac cacccagaag ggccagaaga acagccggga gcggaugaag cggaucgagg     2400

agggcaucaa ggagcugggc agccagaucc ugaaggagca ccccguggag aacacccagc     2460

ugcagaacga gaagcuguac cuguacuacc ugcagaacgg ccgggacaug uacguggacc     2520

aggagcugga caucaaccgg cugagcgacu acgacgugga ccacaucgug ccccagagcu     2580

uccugaagga cgacagcauc gacaacaagg ugcugacccg gagcgacaag aaccggggca     2640

agagcgacaa cgugcccagc gaggaggugg ugaagaagau gaagaacuac uggcggcagc     2700

ugcugaacgc caagcugauc acccagcgga aguucgacaa ccugaccaag gccgagcggg     2760

gcggccugag cgagcuggac aaggccggcu ucaucaagcg gcagcuggug gagacccggc     2820

agaucaccaa gcacguggcc cagauccugg acagccggau gaacaccaag uacgacgaga     2880

acgacaagcu gauccgggag gugaagguga ucacccugaa gagcaagcug gugagcgacu     2940

uccggaagga cuuccaguuc uacaaggugc gggagaucaa caacuaccac cacgcccacg     3000

acgccuaccu gaacgccgug gugggcaccg cccugaucaa gaaguacccc aagcuggaga     3060

gcgaguucgu guacggcgac uacaaggugu acgacgugcg gaagaugauc gccaagagcg     3120

agcaggagau cggcaaggcc accgccaagu acuucuucua cagcaacauc augaacuucu     3180

ucaagaccga gaucacccug gccaacggcg agauccggaa gcggccccug aucgagacca     3240

acggcgagac cggcgagauc gugugggaca agggccggga cuucgccacc gugcggaagg     3300

ugcugagcau gccccaggug aacaucguga agaagaccga ggugcagacc ggcggcuuca     3360

gcaaggagag cauccugccc aagcggaaca gcgacaagcu gaucgcccgg aagaaggacu     3420

gggaccccaa gaaguacggc ggcuucgaca gccccaccgu ggccuacagc gugcuggugg     3480

uggccaaggu ggagaagggc aagagcaaga agcugaagag cgugaaggag cugcugggca     3540

ucaccaucau ggagcggagc agcuucgaga agaaccccau cgacuuccug gaggccaagg     3600

gcuacaagga ggugaagaag gaccugauca ucaagcugcc caaguacagc cuguucgagc     3660

uggagaacgg ccggaagcgg augcuggcca gcgccggcga gcugcagaag ggcaacgagc     3720

uggcccugcc cagcaaguac gugaacuucc uguaccuggc cagccacuac gagaagcuga     3780

agggcagccc cgaggacaac gagcagaagc agcuguucgu ggagcagcac aagcacuacc     3840

uggacgagau caucgagcag aucagcgagu ucagcaagcg ggugauccug gccgacgcca     3900

accuggacaa ggugcugagc gccuacaaca agcaccggga caagcccauc cgggagcagg     3960

ccgagaacau cauccaccug uucacccuga ccaaccuggg cgcccccgcc gccuucaagu     4020

acuucgacac caccaucgac cggaagcggu acaccagcac caaggaggug cuggacgcca     4080

cccugaucca ccagagcauc accggccugu acgagacccg gaucgaccug agccagcugg     4140

gcggcgacgg cggcggcagc cccaagaaga agcggaaggu gugacuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucuc     4320

ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaa                                           4405


<210>  379
<211>  4548
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mRNA transcript with ORF encoding Cas9 with HiBiT tag,
       HSD 5 UTR and human ALB 3 UTR

<400>  379
gggtcccgca gtcggcgtcc agcggctctg cttgttcgtg tgtgtgtcgt tgcaggcctt       60

attcggatcc gccaccatgg acaagaagta cagcatcgga ctggacatcg gaacaaacag      120

cgtcggatgg gcagtcatca cagacgaata caaggtcccg agcaagaagt tcaaggtcct      180

gggaaacaca gacagacaca gcatcaagaa gaacctgatc ggagcactgc tgttcgacag      240

cggagaaaca gcagaagcaa caagactgaa gagaacagca agaagaagat acacaagaag      300

aaagaacaga atctgctacc tgcaggaaat cttcagcaac gaaatggcaa aggtcgacga      360

cagcttcttc cacagactgg aagaaagctt cctggtcgaa gaagacaaga agcacgaaag      420

acacccgatc ttcggaaaca tcgtcgacga agtcgcatac cacgaaaagt acccgacaat      480

ctaccacctg agaaagaagc tggtcgacag cacagacaag gcagacctga gactgatcta      540

cctggcactg gcacacatga tcaagttcag aggacacttc ctgatcgaag gagacctgaa      600

cccggacaac agcgacgtcg acaagctgtt catccagctg gtccagacat acaaccagct      660

gttcgaagaa aacccgatca acgcaagcgg agtcgacgca aaggcaatcc tgagcgcaag      720

actgagcaag agcagaagac tggaaaacct gatcgcacag ctgccgggag aaaagaagaa      780

cggactgttc ggaaacctga tcgcactgag cctgggactg acaccgaact tcaagagcaa      840

cttcgacctg gcagaagacg caaagctgca gctgagcaag gacacatacg acgacgacct      900

ggacaacctg ctggcacaga tcggagacca gtacgcagac ctgttcctgg cagcaaagaa      960

cctgagcgac gcaatcctgc tgagcgacat cctgagagtc aacacagaaa tcacaaaggc     1020

accgctgagc gcaagcatga tcaagagata cgacgaacac caccaggacc tgacactgct     1080

gaaggcactg gtcagacagc agctgccgga aaagtacaag gaaatcttct tcgaccagag     1140

caagaacgga tacgcaggat acatcgacgg aggagcaagc caggaagaat tctacaagtt     1200

catcaagccg atcctggaaa agatggacgg aacagaagaa ctgctggtca agctgaacag     1260

agaagacctg ctgagaaagc agagaacatt cgacaacgga agcatcccgc accagatcca     1320

cctgggagaa ctgcacgcaa tcctgagaag acaggaagac ttctacccgt tcctgaagga     1380

caacagagaa aagatcgaaa agatcctgac attcagaatc ccgtactacg tcggaccgct     1440

ggcaagagga aacagcagat tcgcatggat gacaagaaag agcgaagaaa caatcacacc     1500

gtggaacttc gaagaagtcg tcgacaaggg agcaagcgca cagagcttca tcgaaagaat     1560

gacaaacttc gacaagaacc tgccgaacga aaaggtcctg ccgaagcaca gcctgctgta     1620

cgaatacttc acagtctaca acgaactgac aaaggtcaag tacgtcacag aaggaatgag     1680

aaagccggca ttcctgagcg gagaacagaa gaaggcaatc gtcgacctgc tgttcaagac     1740

aaacagaaag gtcacagtca agcagctgaa ggaagactac ttcaagaaga tcgaatgctt     1800

cgacagcgtc gaaatcagcg gagtcgaaga cagattcaac gcaagcctgg gaacatacca     1860

cgacctgctg aagatcatca aggacaagga cttcctggac aacgaagaaa acgaagacat     1920

cctggaagac atcgtcctga cactgacact gttcgaagac agagaaatga tcgaagaaag     1980

actgaagaca tacgcacacc tgttcgacga caaggtcatg aagcagctga agagaagaag     2040

atacacagga tggggaagac tgagcagaaa gctgatcaac ggaatcagag acaagcagag     2100

cggaaagaca atcctggact tcctgaagag cgacggattc gcaaacagaa acttcatgca     2160

gctgatccac gacgacagcc tgacattcaa ggaagacatc cagaaggcac aggtcagcgg     2220

acagggagac agcctgcacg aacacatcgc aaacctggca ggaagcccgg caatcaagaa     2280

gggaatcctg cagacagtca aggtcgtcga cgaactggtc aaggtcatgg gaagacacaa     2340

gccggaaaac atcgtcatcg aaatggcaag agaaaaccag acaacacaga agggacagaa     2400

gaacagcaga gaaagaatga agagaatcga agaaggaatc aaggaactgg gaagccagat     2460

cctgaaggaa cacccggtcg aaaacacaca gctgcagaac gaaaagctgt acctgtacta     2520

cctgcagaac ggaagagaca tgtacgtcga ccaggaactg gacatcaaca gactgagcga     2580

ctacgacgtc gaccacatcg tcccgcagag cttcctgaag gacgacagca tcgacaacaa     2640

ggtcctgaca agaagcgaca agaacagagg aaagagcgac aacgtcccga gcgaagaagt     2700

cgtcaagaag atgaagaact actggagaca gctgctgaac gcaaagctga tcacacagag     2760

aaagttcgac aacctgacaa aggcagagag aggaggactg agcgaactgg acaaggcagg     2820

attcatcaag agacagctgg tcgaaacaag acagatcaca aagcacgtcg cacagatcct     2880

ggacagcaga atgaacacaa agtacgacga aaacgacaag ctgatcagag aagtcaaggt     2940

catcacactg aagagcaagc tggtcagcga cttcagaaag gacttccagt tctacaaggt     3000

cagagaaatc aacaactacc accacgcaca cgacgcatac ctgaacgcag tcgtcggaac     3060

agcactgatc aagaagtacc cgaagctgga aagcgaattc gtctacggag actacaaggt     3120

ctacgacgtc agaaagatga tcgcaaagag cgaacaggaa atcggaaagg caacagcaaa     3180

gtacttcttc tacagcaaca tcatgaactt cttcaagaca gaaatcacac tggcaaacgg     3240

agaaatcaga aagagaccgc tgatcgaaac aaacggagaa acaggagaaa tcgtctggga     3300

caagggaaga gacttcgcaa cagtcagaaa ggtcctgagc atgccgcagg tcaacatcgt     3360

caagaagaca gaagtccaga caggaggatt cagcaaggaa agcatcctgc cgaagagaaa     3420

cagcgacaag ctgatcgcaa gaaagaagga ctgggacccg aagaagtacg gaggattcga     3480

cagcccgaca gtcgcataca gcgtcctggt cgtcgcaaag gtcgaaaagg gaaagagcaa     3540

gaagctgaag agcgtcaagg aactgctggg aatcacaatc atggaaagaa gcagcttcga     3600

aaagaacccg atcgacttcc tggaagcaaa gggatacaag gaagtcaaga aggacctgat     3660

catcaagctg ccgaagtaca gcctgttcga actggaaaac ggaagaaaga gaatgctggc     3720

aagcgcagga gaactgcaga agggaaacga actggcactg ccgagcaagt acgtcaactt     3780

cctgtacctg gcaagccact acgaaaagct gaagggaagc ccggaagaca acgaacagaa     3840

gcagctgttc gtcgaacagc acaagcacta cctggacgaa atcatcgaac agatcagcga     3900

attcagcaag agagtcatcc tggcagacgc aaacctggac aaggtcctga gcgcatacaa     3960

caagcacaga gacaagccga tcagagaaca ggcagaaaac atcatccacc tgttcacact     4020

gacaaacctg ggagcaccgg cagcattcaa gtacttcgac acaacaatcg acagaaagag     4080

atacacaagc acaaaggaag tcctggacgc aacactgatc caccagagca tcacaggact     4140

gtacgaaaca agaatcgacc tgagccagct gggaggagac ggaggaggaa gcccgaagaa     4200

gaagagaaag gtcagcgaaa gcgcaacacc ggaaagcgtc agcggatgga gactgttcaa     4260

gaagatcagc tagctagcca tcacatttaa aagcatctca gcctaccatg agaataagag     4320

aaagaaaatg aagatcaata gcttattcat ctctttttct ttttcgttgg tgtaaagcca     4380

acaccctgtc taaaaaacat aaatttcttt aatcattttg cctcttttct ctgtgcttca     4440

attaataaaa aatggaaaga acctcgagaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa                  4548


<210>  380
<211>  4522
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mRNA transcript with ORF encoding Cas9 with HiBiT tag,
       CMV-1 5 UTR and human ALB 3 UTR

<400>  380
gggcagatcg cctggagacg ccatccacgc tgttttgacc tccatcgcca ccatggacaa       60

gaagtacagc atcggactgg acatcggaac aaacagcgtc ggatgggcag tcatcacaga      120

cgaatacaag gtcccgagca agaagttcaa ggtcctggga aacacagaca gacacagcat      180

caagaagaac ctgatcggag cactgctgtt cgacagcgga gaaacagcag aagcaacaag      240

actgaagaga acagcaagaa gaagatacac aagaagaaag aacagaatct gctacctgca      300

ggaaatcttc agcaacgaaa tggcaaaggt cgacgacagc ttcttccaca gactggaaga      360

aagcttcctg gtcgaagaag acaagaagca cgaaagacac ccgatcttcg gaaacatcgt      420

cgacgaagtc gcataccacg aaaagtaccc gacaatctac cacctgagaa agaagctggt      480

cgacagcaca gacaaggcag acctgagact gatctacctg gcactggcac acatgatcaa      540

gttcagagga cacttcctga tcgaaggaga cctgaacccg gacaacagcg acgtcgacaa      600

gctgttcatc cagctggtcc agacatacaa ccagctgttc gaagaaaacc cgatcaacgc      660

aagcggagtc gacgcaaagg caatcctgag cgcaagactg agcaagagca gaagactgga      720

aaacctgatc gcacagctgc cgggagaaaa gaagaacgga ctgttcggaa acctgatcgc      780

actgagcctg ggactgacac cgaacttcaa gagcaacttc gacctggcag aagacgcaaa      840

gctgcagctg agcaaggaca catacgacga cgacctggac aacctgctgg cacagatcgg      900

agaccagtac gcagacctgt tcctggcagc aaagaacctg agcgacgcaa tcctgctgag      960

cgacatcctg agagtcaaca cagaaatcac aaaggcaccg ctgagcgcaa gcatgatcaa     1020

gagatacgac gaacaccacc aggacctgac actgctgaag gcactggtca gacagcagct     1080

gccggaaaag tacaaggaaa tcttcttcga ccagagcaag aacggatacg caggatacat     1140

cgacggagga gcaagccagg aagaattcta caagttcatc aagccgatcc tggaaaagat     1200

ggacggaaca gaagaactgc tggtcaagct gaacagagaa gacctgctga gaaagcagag     1260

aacattcgac aacggaagca tcccgcacca gatccacctg ggagaactgc acgcaatcct     1320

gagaagacag gaagacttct acccgttcct gaaggacaac agagaaaaga tcgaaaagat     1380

cctgacattc agaatcccgt actacgtcgg accgctggca agaggaaaca gcagattcgc     1440

atggatgaca agaaagagcg aagaaacaat cacaccgtgg aacttcgaag aagtcgtcga     1500

caagggagca agcgcacaga gcttcatcga aagaatgaca aacttcgaca agaacctgcc     1560

gaacgaaaag gtcctgccga agcacagcct gctgtacgaa tacttcacag tctacaacga     1620

actgacaaag gtcaagtacg tcacagaagg aatgagaaag ccggcattcc tgagcggaga     1680

acagaagaag gcaatcgtcg acctgctgtt caagacaaac agaaaggtca cagtcaagca     1740

gctgaaggaa gactacttca agaagatcga atgcttcgac agcgtcgaaa tcagcggagt     1800

cgaagacaga ttcaacgcaa gcctgggaac ataccacgac ctgctgaaga tcatcaagga     1860

caaggacttc ctggacaacg aagaaaacga agacatcctg gaagacatcg tcctgacact     1920

gacactgttc gaagacagag aaatgatcga agaaagactg aagacatacg cacacctgtt     1980

cgacgacaag gtcatgaagc agctgaagag aagaagatac acaggatggg gaagactgag     2040

cagaaagctg atcaacggaa tcagagacaa gcagagcgga aagacaatcc tggacttcct     2100

gaagagcgac ggattcgcaa acagaaactt catgcagctg atccacgacg acagcctgac     2160

attcaaggaa gacatccaga aggcacaggt cagcggacag ggagacagcc tgcacgaaca     2220

catcgcaaac ctggcaggaa gcccggcaat caagaaggga atcctgcaga cagtcaaggt     2280

cgtcgacgaa ctggtcaagg tcatgggaag acacaagccg gaaaacatcg tcatcgaaat     2340

ggcaagagaa aaccagacaa cacagaaggg acagaagaac agcagagaaa gaatgaagag     2400

aatcgaagaa ggaatcaagg aactgggaag ccagatcctg aaggaacacc cggtcgaaaa     2460

cacacagctg cagaacgaaa agctgtacct gtactacctg cagaacggaa gagacatgta     2520

cgtcgaccag gaactggaca tcaacagact gagcgactac gacgtcgacc acatcgtccc     2580

gcagagcttc ctgaaggacg acagcatcga caacaaggtc ctgacaagaa gcgacaagaa     2640

cagaggaaag agcgacaacg tcccgagcga agaagtcgtc aagaagatga agaactactg     2700

gagacagctg ctgaacgcaa agctgatcac acagagaaag ttcgacaacc tgacaaaggc     2760

agagagagga ggactgagcg aactggacaa ggcaggattc atcaagagac agctggtcga     2820

aacaagacag atcacaaagc acgtcgcaca gatcctggac agcagaatga acacaaagta     2880

cgacgaaaac gacaagctga tcagagaagt caaggtcatc acactgaaga gcaagctggt     2940

cagcgacttc agaaaggact tccagttcta caaggtcaga gaaatcaaca actaccacca     3000

cgcacacgac gcatacctga acgcagtcgt cggaacagca ctgatcaaga agtacccgaa     3060

gctggaaagc gaattcgtct acggagacta caaggtctac gacgtcagaa agatgatcgc     3120

aaagagcgaa caggaaatcg gaaaggcaac agcaaagtac ttcttctaca gcaacatcat     3180

gaacttcttc aagacagaaa tcacactggc aaacggagaa atcagaaaga gaccgctgat     3240

cgaaacaaac ggagaaacag gagaaatcgt ctgggacaag ggaagagact tcgcaacagt     3300

cagaaaggtc ctgagcatgc cgcaggtcaa catcgtcaag aagacagaag tccagacagg     3360

aggattcagc aaggaaagca tcctgccgaa gagaaacagc gacaagctga tcgcaagaaa     3420

gaaggactgg gacccgaaga agtacggagg attcgacagc ccgacagtcg catacagcgt     3480

cctggtcgtc gcaaaggtcg aaaagggaaa gagcaagaag ctgaagagcg tcaaggaact     3540

gctgggaatc acaatcatgg aaagaagcag cttcgaaaag aacccgatcg acttcctgga     3600

agcaaaggga tacaaggaag tcaagaagga cctgatcatc aagctgccga agtacagcct     3660

gttcgaactg gaaaacggaa gaaagagaat gctggcaagc gcaggagaac tgcagaaggg     3720

aaacgaactg gcactgccga gcaagtacgt caacttcctg tacctggcaa gccactacga     3780

aaagctgaag ggaagcccgg aagacaacga acagaagcag ctgttcgtcg aacagcacaa     3840

gcactacctg gacgaaatca tcgaacagat cagcgaattc agcaagagag tcatcctggc     3900

agacgcaaac ctggacaagg tcctgagcgc atacaacaag cacagagaca agccgatcag     3960

agaacaggca gaaaacatca tccacctgtt cacactgaca aacctgggag caccggcagc     4020

attcaagtac ttcgacacaa caatcgacag aaagagatac acaagcacaa aggaagtcct     4080

ggacgcaaca ctgatccacc agagcatcac aggactgtac gaaacaagaa tcgacctgag     4140

ccagctggga ggagacggag gaggaagccc gaagaagaag agaaaggtca gcgaaagcgc     4200

aacaccggaa agcgtcagcg gatggagact gttcaagaag atcagctagc tagccatcac     4260

atttaaaagc atctcagcct accatgagaa taagagaaag aaaatgaaga tcaatagctt     4320

attcatctct ttttcttttt cgttggtgta aagccaacac cctgtctaaa aaacataaat     4380

ttctttaatc attttgcctc ttttctctgt gcttcaatta ataaaaaatg gaaagaacct     4440

cgaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aa                                              4522


<210>  381
<211>  4524
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mRNA transcript with ORF encoding Cas9 with HiBiT tag,
       CMV-2 5 UTR and human ALB 3 UTR

<400>  381
gggagaagac accgggaccg atccagcctc cgcggccggg aacggcgcca ccatggacaa       60

gaagtacagc atcggactgg acatcggaac aaacagcgtc ggatgggcag tcatcacaga      120

cgaatacaag gtcccgagca agaagttcaa ggtcctggga aacacagaca gacacagcat      180

caagaagaac ctgatcggag cactgctgtt cgacagcgga gaaacagcag aagcaacaag      240

actgaagaga acagcaagaa gaagatacac aagaagaaag aacagaatct gctacctgca      300

ggaaatcttc agcaacgaaa tggcaaaggt cgacgacagc ttcttccaca gactggaaga      360

aagcttcctg gtcgaagaag acaagaagca cgaaagacac ccgatcttcg gaaacatcgt      420

cgacgaagtc gcataccacg aaaagtaccc gacaatctac cacctgagaa agaagctggt      480

cgacagcaca gacaaggcag acctgagact gatctacctg gcactggcac acatgatcaa      540

gttcagagga cacttcctga tcgaaggaga cctgaacccg gacaacagcg acgtcgacaa      600

gctgttcatc cagctggtcc agacatacaa ccagctgttc gaagaaaacc cgatcaacgc      660

aagcggagtc gacgcaaagg caatcctgag cgcaagactg agcaagagca gaagactgga      720

aaacctgatc gcacagctgc cgggagaaaa gaagaacgga ctgttcggaa acctgatcgc      780

actgagcctg ggactgacac cgaacttcaa gagcaacttc gacctggcag aagacgcaaa      840

gctgcagctg agcaaggaca catacgacga cgacctggac aacctgctgg cacagatcgg      900

agaccagtac gcagacctgt tcctggcagc aaagaacctg agcgacgcaa tcctgctgag      960

cgacatcctg agagtcaaca cagaaatcac aaaggcaccg ctgagcgcaa gcatgatcaa     1020

gagatacgac gaacaccacc aggacctgac actgctgaag gcactggtca gacagcagct     1080

gccggaaaag tacaaggaaa tcttcttcga ccagagcaag aacggatacg caggatacat     1140

cgacggagga gcaagccagg aagaattcta caagttcatc aagccgatcc tggaaaagat     1200

ggacggaaca gaagaactgc tggtcaagct gaacagagaa gacctgctga gaaagcagag     1260

aacattcgac aacggaagca tcccgcacca gatccacctg ggagaactgc acgcaatcct     1320

gagaagacag gaagacttct acccgttcct gaaggacaac agagaaaaga tcgaaaagat     1380

cctgacattc agaatcccgt actacgtcgg accgctggca agaggaaaca gcagattcgc     1440

atggatgaca agaaagagcg aagaaacaat cacaccgtgg aacttcgaag aagtcgtcga     1500

caagggagca agcgcacaga gcttcatcga aagaatgaca aacttcgaca agaacctgcc     1560

gaacgaaaag gtcctgccga agcacagcct gctgtacgaa tacttcacag tctacaacga     1620

actgacaaag gtcaagtacg tcacagaagg aatgagaaag ccggcattcc tgagcggaga     1680

acagaagaag gcaatcgtcg acctgctgtt caagacaaac agaaaggtca cagtcaagca     1740

gctgaaggaa gactacttca agaagatcga atgcttcgac agcgtcgaaa tcagcggagt     1800

cgaagacaga ttcaacgcaa gcctgggaac ataccacgac ctgctgaaga tcatcaagga     1860

caaggacttc ctggacaacg aagaaaacga agacatcctg gaagacatcg tcctgacact     1920

gacactgttc gaagacagag aaatgatcga agaaagactg aagacatacg cacacctgtt     1980

cgacgacaag gtcatgaagc agctgaagag aagaagatac acaggatggg gaagactgag     2040

cagaaagctg atcaacggaa tcagagacaa gcagagcgga aagacaatcc tggacttcct     2100

gaagagcgac ggattcgcaa acagaaactt catgcagctg atccacgacg acagcctgac     2160

attcaaggaa gacatccaga aggcacaggt cagcggacag ggagacagcc tgcacgaaca     2220

catcgcaaac ctggcaggaa gcccggcaat caagaaggga atcctgcaga cagtcaaggt     2280

cgtcgacgaa ctggtcaagg tcatgggaag acacaagccg gaaaacatcg tcatcgaaat     2340

ggcaagagaa aaccagacaa cacagaaggg acagaagaac agcagagaaa gaatgaagag     2400

aatcgaagaa ggaatcaagg aactgggaag ccagatcctg aaggaacacc cggtcgaaaa     2460

cacacagctg cagaacgaaa agctgtacct gtactacctg cagaacggaa gagacatgta     2520

cgtcgaccag gaactggaca tcaacagact gagcgactac gacgtcgacc acatcgtccc     2580

gcagagcttc ctgaaggacg acagcatcga caacaaggtc ctgacaagaa gcgacaagaa     2640

cagaggaaag agcgacaacg tcccgagcga agaagtcgtc aagaagatga agaactactg     2700

gagacagctg ctgaacgcaa agctgatcac acagagaaag ttcgacaacc tgacaaaggc     2760

agagagagga ggactgagcg aactggacaa ggcaggattc atcaagagac agctggtcga     2820

aacaagacag atcacaaagc acgtcgcaca gatcctggac agcagaatga acacaaagta     2880

cgacgaaaac gacaagctga tcagagaagt caaggtcatc acactgaaga gcaagctggt     2940

cagcgacttc agaaaggact tccagttcta caaggtcaga gaaatcaaca actaccacca     3000

cgcacacgac gcatacctga acgcagtcgt cggaacagca ctgatcaaga agtacccgaa     3060

gctggaaagc gaattcgtct acggagacta caaggtctac gacgtcagaa agatgatcgc     3120

aaagagcgaa caggaaatcg gaaaggcaac agcaaagtac ttcttctaca gcaacatcat     3180

gaacttcttc aagacagaaa tcacactggc aaacggagaa atcagaaaga gaccgctgat     3240

cgaaacaaac ggagaaacag gagaaatcgt ctgggacaag ggaagagact tcgcaacagt     3300

cagaaaggtc ctgagcatgc cgcaggtcaa catcgtcaag aagacagaag tccagacagg     3360

aggattcagc aaggaaagca tcctgccgaa gagaaacagc gacaagctga tcgcaagaaa     3420

gaaggactgg gacccgaaga agtacggagg attcgacagc ccgacagtcg catacagcgt     3480

cctggtcgtc gcaaaggtcg aaaagggaaa gagcaagaag ctgaagagcg tcaaggaact     3540

gctgggaatc acaatcatgg aaagaagcag cttcgaaaag aacccgatcg acttcctgga     3600

agcaaaggga tacaaggaag tcaagaagga cctgatcatc aagctgccga agtacagcct     3660

gttcgaactg gaaaacggaa gaaagagaat gctggcaagc gcaggagaac tgcagaaggg     3720

aaacgaactg gcactgccga gcaagtacgt caacttcctg tacctggcaa gccactacga     3780

aaagctgaag ggaagcccgg aagacaacga acagaagcag ctgttcgtcg aacagcacaa     3840

gcactacctg gacgaaatca tcgaacagat cagcgaattc agcaagagag tcatcctggc     3900

agacgcaaac ctggacaagg tcctgagcgc atacaacaag cacagagaca agccgatcag     3960

agaacaggca gaaaacatca tccacctgtt cacactgaca aacctgggag caccggcagc     4020

attcaagtac ttcgacacaa caatcgacag aaagagatac acaagcacaa aggaagtcct     4080

ggacgcaaca ctgatccacc agagcatcac aggactgtac gaaacaagaa tcgacctgag     4140

ccagctggga ggagacggag gaggaagccc gaagaagaag agaaaggtca gcgaaagcgc     4200

aacaccggaa agcgtcagcg gatggagact gttcaagaag atcagctagc tagccatcac     4260

atttaaaagc atctcagcct accatgagaa taagagaaag aaaatgaaga tcaatagctt     4320

attcatctct ttttcttttt cgttggtgta aagccaacac cctgtctaaa aaacataaat     4380

ttctttaatc attttgcctc ttttctctgt gcttcaatta ataaaaaatg gaaagaacct     4440

cgagaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaa                                            4524


<210>  382
<211>  4524
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mRNA transcript with ORF encoding Cas9 with HiBiT tag,
       CMV-3 5 UTR and human ALB 3 UTR

<400>  382
gggtgcattg gaacgcggat tccccgtgcc aagagtgact caccgcgcca ccatggacaa       60

gaagtacagc atcggactgg acatcggaac aaacagcgtc ggatgggcag tcatcacaga      120

cgaatacaag gtcccgagca agaagttcaa ggtcctggga aacacagaca gacacagcat      180

caagaagaac ctgatcggag cactgctgtt cgacagcgga gaaacagcag aagcaacaag      240

actgaagaga acagcaagaa gaagatacac aagaagaaag aacagaatct gctacctgca      300

ggaaatcttc agcaacgaaa tggcaaaggt cgacgacagc ttcttccaca gactggaaga      360

aagcttcctg gtcgaagaag acaagaagca cgaaagacac ccgatcttcg gaaacatcgt      420

cgacgaagtc gcataccacg aaaagtaccc gacaatctac cacctgagaa agaagctggt      480

cgacagcaca gacaaggcag acctgagact gatctacctg gcactggcac acatgatcaa      540

gttcagagga cacttcctga tcgaaggaga cctgaacccg gacaacagcg acgtcgacaa      600

gctgttcatc cagctggtcc agacatacaa ccagctgttc gaagaaaacc cgatcaacgc      660

aagcggagtc gacgcaaagg caatcctgag cgcaagactg agcaagagca gaagactgga      720

aaacctgatc gcacagctgc cgggagaaaa gaagaacgga ctgttcggaa acctgatcgc      780

actgagcctg ggactgacac cgaacttcaa gagcaacttc gacctggcag aagacgcaaa      840

gctgcagctg agcaaggaca catacgacga cgacctggac aacctgctgg cacagatcgg      900

agaccagtac gcagacctgt tcctggcagc aaagaacctg agcgacgcaa tcctgctgag      960

cgacatcctg agagtcaaca cagaaatcac aaaggcaccg ctgagcgcaa gcatgatcaa     1020

gagatacgac gaacaccacc aggacctgac actgctgaag gcactggtca gacagcagct     1080

gccggaaaag tacaaggaaa tcttcttcga ccagagcaag aacggatacg caggatacat     1140

cgacggagga gcaagccagg aagaattcta caagttcatc aagccgatcc tggaaaagat     1200

ggacggaaca gaagaactgc tggtcaagct gaacagagaa gacctgctga gaaagcagag     1260

aacattcgac aacggaagca tcccgcacca gatccacctg ggagaactgc acgcaatcct     1320

gagaagacag gaagacttct acccgttcct gaaggacaac agagaaaaga tcgaaaagat     1380

cctgacattc agaatcccgt actacgtcgg accgctggca agaggaaaca gcagattcgc     1440

atggatgaca agaaagagcg aagaaacaat cacaccgtgg aacttcgaag aagtcgtcga     1500

caagggagca agcgcacaga gcttcatcga aagaatgaca aacttcgaca agaacctgcc     1560

gaacgaaaag gtcctgccga agcacagcct gctgtacgaa tacttcacag tctacaacga     1620

actgacaaag gtcaagtacg tcacagaagg aatgagaaag ccggcattcc tgagcggaga     1680

acagaagaag gcaatcgtcg acctgctgtt caagacaaac agaaaggtca cagtcaagca     1740

gctgaaggaa gactacttca agaagatcga atgcttcgac agcgtcgaaa tcagcggagt     1800

cgaagacaga ttcaacgcaa gcctgggaac ataccacgac ctgctgaaga tcatcaagga     1860

caaggacttc ctggacaacg aagaaaacga agacatcctg gaagacatcg tcctgacact     1920

gacactgttc gaagacagag aaatgatcga agaaagactg aagacatacg cacacctgtt     1980

cgacgacaag gtcatgaagc agctgaagag aagaagatac acaggatggg gaagactgag     2040

cagaaagctg atcaacggaa tcagagacaa gcagagcgga aagacaatcc tggacttcct     2100

gaagagcgac ggattcgcaa acagaaactt catgcagctg atccacgacg acagcctgac     2160

attcaaggaa gacatccaga aggcacaggt cagcggacag ggagacagcc tgcacgaaca     2220

catcgcaaac ctggcaggaa gcccggcaat caagaaggga atcctgcaga cagtcaaggt     2280

cgtcgacgaa ctggtcaagg tcatgggaag acacaagccg gaaaacatcg tcatcgaaat     2340

ggcaagagaa aaccagacaa cacagaaggg acagaagaac agcagagaaa gaatgaagag     2400

aatcgaagaa ggaatcaagg aactgggaag ccagatcctg aaggaacacc cggtcgaaaa     2460

cacacagctg cagaacgaaa agctgtacct gtactacctg cagaacggaa gagacatgta     2520

cgtcgaccag gaactggaca tcaacagact gagcgactac gacgtcgacc acatcgtccc     2580

gcagagcttc ctgaaggacg acagcatcga caacaaggtc ctgacaagaa gcgacaagaa     2640

cagaggaaag agcgacaacg tcccgagcga agaagtcgtc aagaagatga agaactactg     2700

gagacagctg ctgaacgcaa agctgatcac acagagaaag ttcgacaacc tgacaaaggc     2760

agagagagga ggactgagcg aactggacaa ggcaggattc atcaagagac agctggtcga     2820

aacaagacag atcacaaagc acgtcgcaca gatcctggac agcagaatga acacaaagta     2880

cgacgaaaac gacaagctga tcagagaagt caaggtcatc acactgaaga gcaagctggt     2940

cagcgacttc agaaaggact tccagttcta caaggtcaga gaaatcaaca actaccacca     3000

cgcacacgac gcatacctga acgcagtcgt cggaacagca ctgatcaaga agtacccgaa     3060

gctggaaagc gaattcgtct acggagacta caaggtctac gacgtcagaa agatgatcgc     3120

aaagagcgaa caggaaatcg gaaaggcaac agcaaagtac ttcttctaca gcaacatcat     3180

gaacttcttc aagacagaaa tcacactggc aaacggagaa atcagaaaga gaccgctgat     3240

cgaaacaaac ggagaaacag gagaaatcgt ctgggacaag ggaagagact tcgcaacagt     3300

cagaaaggtc ctgagcatgc cgcaggtcaa catcgtcaag aagacagaag tccagacagg     3360

aggattcagc aaggaaagca tcctgccgaa gagaaacagc gacaagctga tcgcaagaaa     3420

gaaggactgg gacccgaaga agtacggagg attcgacagc ccgacagtcg catacagcgt     3480

cctggtcgtc gcaaaggtcg aaaagggaaa gagcaagaag ctgaagagcg tcaaggaact     3540

gctgggaatc acaatcatgg aaagaagcag cttcgaaaag aacccgatcg acttcctgga     3600

agcaaaggga tacaaggaag tcaagaagga cctgatcatc aagctgccga agtacagcct     3660

gttcgaactg gaaaacggaa gaaagagaat gctggcaagc gcaggagaac tgcagaaggg     3720

aaacgaactg gcactgccga gcaagtacgt caacttcctg tacctggcaa gccactacga     3780

aaagctgaag ggaagcccgg aagacaacga acagaagcag ctgttcgtcg aacagcacaa     3840

gcactacctg gacgaaatca tcgaacagat cagcgaattc agcaagagag tcatcctggc     3900

agacgcaaac ctggacaagg tcctgagcgc atacaacaag cacagagaca agccgatcag     3960

agaacaggca gaaaacatca tccacctgtt cacactgaca aacctgggag caccggcagc     4020

attcaagtac ttcgacacaa caatcgacag aaagagatac acaagcacaa aggaagtcct     4080

ggacgcaaca ctgatccacc agagcatcac aggactgtac gaaacaagaa tcgacctgag     4140

ccagctggga ggagacggag gaggaagccc gaagaagaag agaaaggtca gcgaaagcgc     4200

aacaccggaa agcgtcagcg gatggagact gttcaagaag atcagctagc tagccatcac     4260

atttaaaagc atctcagcct accatgagaa taagagaaag aaaatgaaga tcaatagctt     4320

attcatctct ttttcttttt cgttggtgta aagccaacac cctgtctaaa aaacataaat     4380

ttctttaatc attttgcctc ttttctctgt gcttcaatta ataaaaaatg gaaagaacct     4440

cgagaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaa                                            4524


<210>  383
<211>  4548
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mRNA transcript with ORF encoding Cas9 with HiBiT tag,
       HBA 5 UTR and human ALB 3 UTR

<400>  383
gggcataaac cctggcgcgc tcgcggcccg gcactcttct ggtccccaca gactcagaga       60

gaacccaccc gccaccatgg acaagaagta cagcatcgga ctggacatcg gaacaaacag      120

cgtcggatgg gcagtcatca cagacgaata caaggtcccg agcaagaagt tcaaggtcct      180

gggaaacaca gacagacaca gcatcaagaa gaacctgatc ggagcactgc tgttcgacag      240

cggagaaaca gcagaagcaa caagactgaa gagaacagca agaagaagat acacaagaag      300

aaagaacaga atctgctacc tgcaggaaat cttcagcaac gaaatggcaa aggtcgacga      360

cagcttcttc cacagactgg aagaaagctt cctggtcgaa gaagacaaga agcacgaaag      420

acacccgatc ttcggaaaca tcgtcgacga agtcgcatac cacgaaaagt acccgacaat      480

ctaccacctg agaaagaagc tggtcgacag cacagacaag gcagacctga gactgatcta      540

cctggcactg gcacacatga tcaagttcag aggacacttc ctgatcgaag gagacctgaa      600

cccggacaac agcgacgtcg acaagctgtt catccagctg gtccagacat acaaccagct      660

gttcgaagaa aacccgatca acgcaagcgg agtcgacgca aaggcaatcc tgagcgcaag      720

actgagcaag agcagaagac tggaaaacct gatcgcacag ctgccgggag aaaagaagaa      780

cggactgttc ggaaacctga tcgcactgag cctgggactg acaccgaact tcaagagcaa      840

cttcgacctg gcagaagacg caaagctgca gctgagcaag gacacatacg acgacgacct      900

ggacaacctg ctggcacaga tcggagacca gtacgcagac ctgttcctgg cagcaaagaa      960

cctgagcgac gcaatcctgc tgagcgacat cctgagagtc aacacagaaa tcacaaaggc     1020

accgctgagc gcaagcatga tcaagagata cgacgaacac caccaggacc tgacactgct     1080

gaaggcactg gtcagacagc agctgccgga aaagtacaag gaaatcttct tcgaccagag     1140

caagaacgga tacgcaggat acatcgacgg aggagcaagc caggaagaat tctacaagtt     1200

catcaagccg atcctggaaa agatggacgg aacagaagaa ctgctggtca agctgaacag     1260

agaagacctg ctgagaaagc agagaacatt cgacaacgga agcatcccgc accagatcca     1320

cctgggagaa ctgcacgcaa tcctgagaag acaggaagac ttctacccgt tcctgaagga     1380

caacagagaa aagatcgaaa agatcctgac attcagaatc ccgtactacg tcggaccgct     1440

ggcaagagga aacagcagat tcgcatggat gacaagaaag agcgaagaaa caatcacacc     1500

gtggaacttc gaagaagtcg tcgacaaggg agcaagcgca cagagcttca tcgaaagaat     1560

gacaaacttc gacaagaacc tgccgaacga aaaggtcctg ccgaagcaca gcctgctgta     1620

cgaatacttc acagtctaca acgaactgac aaaggtcaag tacgtcacag aaggaatgag     1680

aaagccggca ttcctgagcg gagaacagaa gaaggcaatc gtcgacctgc tgttcaagac     1740

aaacagaaag gtcacagtca agcagctgaa ggaagactac ttcaagaaga tcgaatgctt     1800

cgacagcgtc gaaatcagcg gagtcgaaga cagattcaac gcaagcctgg gaacatacca     1860

cgacctgctg aagatcatca aggacaagga cttcctggac aacgaagaaa acgaagacat     1920

cctggaagac atcgtcctga cactgacact gttcgaagac agagaaatga tcgaagaaag     1980

actgaagaca tacgcacacc tgttcgacga caaggtcatg aagcagctga agagaagaag     2040

atacacagga tggggaagac tgagcagaaa gctgatcaac ggaatcagag acaagcagag     2100

cggaaagaca atcctggact tcctgaagag cgacggattc gcaaacagaa acttcatgca     2160

gctgatccac gacgacagcc tgacattcaa ggaagacatc cagaaggcac aggtcagcgg     2220

acagggagac agcctgcacg aacacatcgc aaacctggca ggaagcccgg caatcaagaa     2280

gggaatcctg cagacagtca aggtcgtcga cgaactggtc aaggtcatgg gaagacacaa     2340

gccggaaaac atcgtcatcg aaatggcaag agaaaaccag acaacacaga agggacagaa     2400

gaacagcaga gaaagaatga agagaatcga agaaggaatc aaggaactgg gaagccagat     2460

cctgaaggaa cacccggtcg aaaacacaca gctgcagaac gaaaagctgt acctgtacta     2520

cctgcagaac ggaagagaca tgtacgtcga ccaggaactg gacatcaaca gactgagcga     2580

ctacgacgtc gaccacatcg tcccgcagag cttcctgaag gacgacagca tcgacaacaa     2640

ggtcctgaca agaagcgaca agaacagagg aaagagcgac aacgtcccga gcgaagaagt     2700

cgtcaagaag atgaagaact actggagaca gctgctgaac gcaaagctga tcacacagag     2760

aaagttcgac aacctgacaa aggcagagag aggaggactg agcgaactgg acaaggcagg     2820

attcatcaag agacagctgg tcgaaacaag acagatcaca aagcacgtcg cacagatcct     2880

ggacagcaga atgaacacaa agtacgacga aaacgacaag ctgatcagag aagtcaaggt     2940

catcacactg aagagcaagc tggtcagcga cttcagaaag gacttccagt tctacaaggt     3000

cagagaaatc aacaactacc accacgcaca cgacgcatac ctgaacgcag tcgtcggaac     3060

agcactgatc aagaagtacc cgaagctgga aagcgaattc gtctacggag actacaaggt     3120

ctacgacgtc agaaagatga tcgcaaagag cgaacaggaa atcggaaagg caacagcaaa     3180

gtacttcttc tacagcaaca tcatgaactt cttcaagaca gaaatcacac tggcaaacgg     3240

agaaatcaga aagagaccgc tgatcgaaac aaacggagaa acaggagaaa tcgtctggga     3300

caagggaaga gacttcgcaa cagtcagaaa ggtcctgagc atgccgcagg tcaacatcgt     3360

caagaagaca gaagtccaga caggaggatt cagcaaggaa agcatcctgc cgaagagaaa     3420

cagcgacaag ctgatcgcaa gaaagaagga ctgggacccg aagaagtacg gaggattcga     3480

cagcccgaca gtcgcataca gcgtcctggt cgtcgcaaag gtcgaaaagg gaaagagcaa     3540

gaagctgaag agcgtcaagg aactgctggg aatcacaatc atggaaagaa gcagcttcga     3600

aaagaacccg atcgacttcc tggaagcaaa gggatacaag gaagtcaaga aggacctgat     3660

catcaagctg ccgaagtaca gcctgttcga actggaaaac ggaagaaaga gaatgctggc     3720

aagcgcagga gaactgcaga agggaaacga actggcactg ccgagcaagt acgtcaactt     3780

cctgtacctg gcaagccact acgaaaagct gaagggaagc ccggaagaca acgaacagaa     3840

gcagctgttc gtcgaacagc acaagcacta cctggacgaa atcatcgaac agatcagcga     3900

attcagcaag agagtcatcc tggcagacgc aaacctggac aaggtcctga gcgcatacaa     3960

caagcacaga gacaagccga tcagagaaca ggcagaaaac atcatccacc tgttcacact     4020

gacaaacctg ggagcaccgg cagcattcaa gtacttcgac acaacaatcg acagaaagag     4080

atacacaagc acaaaggaag tcctggacgc aacactgatc caccagagca tcacaggact     4140

gtacgaaaca agaatcgacc tgagccagct gggaggagac ggaggaggaa gcccgaagaa     4200

gaagagaaag gtcagcgaaa gcgcaacacc ggaaagcgtc agcggatgga gactgttcaa     4260

gaagatcagc tagctagcca tcacatttaa aagcatctca gcctaccatg agaataagag     4320

aaagaaaatg aagatcaata gcttattcat ctctttttct ttttcgttgg tgtaaagcca     4380

acaccctgtc taaaaaacat aaatttcttt aatcattttg cctcttttct ctgtgcttca     4440

attaataaaa aatggaaaga acctcgagaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa                  4548


<210>  384
<211>  4538
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mRNA transcript with ORF encoding Cas9 with HiBiT tag,
       HBB 5 UTR and human ALB 3 UTR

<400>  384
gggacatttg cttctgacac aactgtgttc actagcaacc tcaaacagac accggatctc       60

gccaccatgg acaagaagta cagcatcgga ctggacatcg gaacaaacag cgtcggatgg      120

gcagtcatca cagacgaata caaggtcccg agcaagaagt tcaaggtcct gggaaacaca      180

gacagacaca gcatcaagaa gaacctgatc ggagcactgc tgttcgacag cggagaaaca      240

gcagaagcaa caagactgaa gagaacagca agaagaagat acacaagaag aaagaacaga      300

atctgctacc tgcaggaaat cttcagcaac gaaatggcaa aggtcgacga cagcttcttc      360

cacagactgg aagaaagctt cctggtcgaa gaagacaaga agcacgaaag acacccgatc      420

ttcggaaaca tcgtcgacga agtcgcatac cacgaaaagt acccgacaat ctaccacctg      480

agaaagaagc tggtcgacag cacagacaag gcagacctga gactgatcta cctggcactg      540

gcacacatga tcaagttcag aggacacttc ctgatcgaag gagacctgaa cccggacaac      600

agcgacgtcg acaagctgtt catccagctg gtccagacat acaaccagct gttcgaagaa      660

aacccgatca acgcaagcgg agtcgacgca aaggcaatcc tgagcgcaag actgagcaag      720

agcagaagac tggaaaacct gatcgcacag ctgccgggag aaaagaagaa cggactgttc      780

ggaaacctga tcgcactgag cctgggactg acaccgaact tcaagagcaa cttcgacctg      840

gcagaagacg caaagctgca gctgagcaag gacacatacg acgacgacct ggacaacctg      900

ctggcacaga tcggagacca gtacgcagac ctgttcctgg cagcaaagaa cctgagcgac      960

gcaatcctgc tgagcgacat cctgagagtc aacacagaaa tcacaaaggc accgctgagc     1020

gcaagcatga tcaagagata cgacgaacac caccaggacc tgacactgct gaaggcactg     1080

gtcagacagc agctgccgga aaagtacaag gaaatcttct tcgaccagag caagaacgga     1140

tacgcaggat acatcgacgg aggagcaagc caggaagaat tctacaagtt catcaagccg     1200

atcctggaaa agatggacgg aacagaagaa ctgctggtca agctgaacag agaagacctg     1260

ctgagaaagc agagaacatt cgacaacgga agcatcccgc accagatcca cctgggagaa     1320

ctgcacgcaa tcctgagaag acaggaagac ttctacccgt tcctgaagga caacagagaa     1380

aagatcgaaa agatcctgac attcagaatc ccgtactacg tcggaccgct ggcaagagga     1440

aacagcagat tcgcatggat gacaagaaag agcgaagaaa caatcacacc gtggaacttc     1500

gaagaagtcg tcgacaaggg agcaagcgca cagagcttca tcgaaagaat gacaaacttc     1560

gacaagaacc tgccgaacga aaaggtcctg ccgaagcaca gcctgctgta cgaatacttc     1620

acagtctaca acgaactgac aaaggtcaag tacgtcacag aaggaatgag aaagccggca     1680

ttcctgagcg gagaacagaa gaaggcaatc gtcgacctgc tgttcaagac aaacagaaag     1740

gtcacagtca agcagctgaa ggaagactac ttcaagaaga tcgaatgctt cgacagcgtc     1800

gaaatcagcg gagtcgaaga cagattcaac gcaagcctgg gaacatacca cgacctgctg     1860

aagatcatca aggacaagga cttcctggac aacgaagaaa acgaagacat cctggaagac     1920

atcgtcctga cactgacact gttcgaagac agagaaatga tcgaagaaag actgaagaca     1980

tacgcacacc tgttcgacga caaggtcatg aagcagctga agagaagaag atacacagga     2040

tggggaagac tgagcagaaa gctgatcaac ggaatcagag acaagcagag cggaaagaca     2100

atcctggact tcctgaagag cgacggattc gcaaacagaa acttcatgca gctgatccac     2160

gacgacagcc tgacattcaa ggaagacatc cagaaggcac aggtcagcgg acagggagac     2220

agcctgcacg aacacatcgc aaacctggca ggaagcccgg caatcaagaa gggaatcctg     2280

cagacagtca aggtcgtcga cgaactggtc aaggtcatgg gaagacacaa gccggaaaac     2340

atcgtcatcg aaatggcaag agaaaaccag acaacacaga agggacagaa gaacagcaga     2400

gaaagaatga agagaatcga agaaggaatc aaggaactgg gaagccagat cctgaaggaa     2460

cacccggtcg aaaacacaca gctgcagaac gaaaagctgt acctgtacta cctgcagaac     2520

ggaagagaca tgtacgtcga ccaggaactg gacatcaaca gactgagcga ctacgacgtc     2580

gaccacatcg tcccgcagag cttcctgaag gacgacagca tcgacaacaa ggtcctgaca     2640

agaagcgaca agaacagagg aaagagcgac aacgtcccga gcgaagaagt cgtcaagaag     2700

atgaagaact actggagaca gctgctgaac gcaaagctga tcacacagag aaagttcgac     2760

aacctgacaa aggcagagag aggaggactg agcgaactgg acaaggcagg attcatcaag     2820

agacagctgg tcgaaacaag acagatcaca aagcacgtcg cacagatcct ggacagcaga     2880

atgaacacaa agtacgacga aaacgacaag ctgatcagag aagtcaaggt catcacactg     2940

aagagcaagc tggtcagcga cttcagaaag gacttccagt tctacaaggt cagagaaatc     3000

aacaactacc accacgcaca cgacgcatac ctgaacgcag tcgtcggaac agcactgatc     3060

aagaagtacc cgaagctgga aagcgaattc gtctacggag actacaaggt ctacgacgtc     3120

agaaagatga tcgcaaagag cgaacaggaa atcggaaagg caacagcaaa gtacttcttc     3180

tacagcaaca tcatgaactt cttcaagaca gaaatcacac tggcaaacgg agaaatcaga     3240

aagagaccgc tgatcgaaac aaacggagaa acaggagaaa tcgtctggga caagggaaga     3300

gacttcgcaa cagtcagaaa ggtcctgagc atgccgcagg tcaacatcgt caagaagaca     3360

gaagtccaga caggaggatt cagcaaggaa agcatcctgc cgaagagaaa cagcgacaag     3420

ctgatcgcaa gaaagaagga ctgggacccg aagaagtacg gaggattcga cagcccgaca     3480

gtcgcataca gcgtcctggt cgtcgcaaag gtcgaaaagg gaaagagcaa gaagctgaag     3540

agcgtcaagg aactgctggg aatcacaatc atggaaagaa gcagcttcga aaagaacccg     3600

atcgacttcc tggaagcaaa gggatacaag gaagtcaaga aggacctgat catcaagctg     3660

ccgaagtaca gcctgttcga actggaaaac ggaagaaaga gaatgctggc aagcgcagga     3720

gaactgcaga agggaaacga actggcactg ccgagcaagt acgtcaactt cctgtacctg     3780

gcaagccact acgaaaagct gaagggaagc ccggaagaca acgaacagaa gcagctgttc     3840

gtcgaacagc acaagcacta cctggacgaa atcatcgaac agatcagcga attcagcaag     3900

agagtcatcc tggcagacgc aaacctggac aaggtcctga gcgcatacaa caagcacaga     3960

gacaagccga tcagagaaca ggcagaaaac atcatccacc tgttcacact gacaaacctg     4020

ggagcaccgg cagcattcaa gtacttcgac acaacaatcg acagaaagag atacacaagc     4080

acaaaggaag tcctggacgc aacactgatc caccagagca tcacaggact gtacgaaaca     4140

agaatcgacc tgagccagct gggaggagac ggaggaggaa gcccgaagaa gaagagaaag     4200

gtcagcgaaa gcgcaacacc ggaaagcgtc agcggatgga gactgttcaa gaagatcagc     4260

tagctagcca tcacatttaa aagcatctca gcctaccatg agaataagag aaagaaaatg     4320

aagatcaata gcttattcat ctctttttct ttttcgttgg tgtaaagcca acaccctgtc     4380

taaaaaacat aaatttcttt aatcattttg cctcttttct ctgtgcttca attaataaaa     4440

aatggaaaga acctcgagaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa                             4538


<210>  385
<211>  4517
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mRNA transcript with ORF encoding Cas9 with HiBiT tag,
       XBG 5 UTR and human ALB 3 UTR

<400>  385
gggaagctca gaataaacgc tcaactttgg ccggatctcg ccaccatgga caagaagtac       60

agcatcggac tggacatcgg aacaaacagc gtcggatggg cagtcatcac agacgaatac      120

aaggtcccga gcaagaagtt caaggtcctg ggaaacacag acagacacag catcaagaag      180

aacctgatcg gagcactgct gttcgacagc ggagaaacag cagaagcaac aagactgaag      240

agaacagcaa gaagaagata cacaagaaga aagaacagaa tctgctacct gcaggaaatc      300

ttcagcaacg aaatggcaaa ggtcgacgac agcttcttcc acagactgga agaaagcttc      360

ctggtcgaag aagacaagaa gcacgaaaga cacccgatct tcggaaacat cgtcgacgaa      420

gtcgcatacc acgaaaagta cccgacaatc taccacctga gaaagaagct ggtcgacagc      480

acagacaagg cagacctgag actgatctac ctggcactgg cacacatgat caagttcaga      540

ggacacttcc tgatcgaagg agacctgaac ccggacaaca gcgacgtcga caagctgttc      600

atccagctgg tccagacata caaccagctg ttcgaagaaa acccgatcaa cgcaagcgga      660

gtcgacgcaa aggcaatcct gagcgcaaga ctgagcaaga gcagaagact ggaaaacctg      720

atcgcacagc tgccgggaga aaagaagaac ggactgttcg gaaacctgat cgcactgagc      780

ctgggactga caccgaactt caagagcaac ttcgacctgg cagaagacgc aaagctgcag      840

ctgagcaagg acacatacga cgacgacctg gacaacctgc tggcacagat cggagaccag      900

tacgcagacc tgttcctggc agcaaagaac ctgagcgacg caatcctgct gagcgacatc      960

ctgagagtca acacagaaat cacaaaggca ccgctgagcg caagcatgat caagagatac     1020

gacgaacacc accaggacct gacactgctg aaggcactgg tcagacagca gctgccggaa     1080

aagtacaagg aaatcttctt cgaccagagc aagaacggat acgcaggata catcgacgga     1140

ggagcaagcc aggaagaatt ctacaagttc atcaagccga tcctggaaaa gatggacgga     1200

acagaagaac tgctggtcaa gctgaacaga gaagacctgc tgagaaagca gagaacattc     1260

gacaacggaa gcatcccgca ccagatccac ctgggagaac tgcacgcaat cctgagaaga     1320

caggaagact tctacccgtt cctgaaggac aacagagaaa agatcgaaaa gatcctgaca     1380

ttcagaatcc cgtactacgt cggaccgctg gcaagaggaa acagcagatt cgcatggatg     1440

acaagaaaga gcgaagaaac aatcacaccg tggaacttcg aagaagtcgt cgacaaggga     1500

gcaagcgcac agagcttcat cgaaagaatg acaaacttcg acaagaacct gccgaacgaa     1560

aaggtcctgc cgaagcacag cctgctgtac gaatacttca cagtctacaa cgaactgaca     1620

aaggtcaagt acgtcacaga aggaatgaga aagccggcat tcctgagcgg agaacagaag     1680

aaggcaatcg tcgacctgct gttcaagaca aacagaaagg tcacagtcaa gcagctgaag     1740

gaagactact tcaagaagat cgaatgcttc gacagcgtcg aaatcagcgg agtcgaagac     1800

agattcaacg caagcctggg aacataccac gacctgctga agatcatcaa ggacaaggac     1860

ttcctggaca acgaagaaaa cgaagacatc ctggaagaca tcgtcctgac actgacactg     1920

ttcgaagaca gagaaatgat cgaagaaaga ctgaagacat acgcacacct gttcgacgac     1980

aaggtcatga agcagctgaa gagaagaaga tacacaggat ggggaagact gagcagaaag     2040

ctgatcaacg gaatcagaga caagcagagc ggaaagacaa tcctggactt cctgaagagc     2100

gacggattcg caaacagaaa cttcatgcag ctgatccacg acgacagcct gacattcaag     2160

gaagacatcc agaaggcaca ggtcagcgga cagggagaca gcctgcacga acacatcgca     2220

aacctggcag gaagcccggc aatcaagaag ggaatcctgc agacagtcaa ggtcgtcgac     2280

gaactggtca aggtcatggg aagacacaag ccggaaaaca tcgtcatcga aatggcaaga     2340

gaaaaccaga caacacagaa gggacagaag aacagcagag aaagaatgaa gagaatcgaa     2400

gaaggaatca aggaactggg aagccagatc ctgaaggaac acccggtcga aaacacacag     2460

ctgcagaacg aaaagctgta cctgtactac ctgcagaacg gaagagacat gtacgtcgac     2520

caggaactgg acatcaacag actgagcgac tacgacgtcg accacatcgt cccgcagagc     2580

ttcctgaagg acgacagcat cgacaacaag gtcctgacaa gaagcgacaa gaacagagga     2640

aagagcgaca acgtcccgag cgaagaagtc gtcaagaaga tgaagaacta ctggagacag     2700

ctgctgaacg caaagctgat cacacagaga aagttcgaca acctgacaaa ggcagagaga     2760

ggaggactga gcgaactgga caaggcagga ttcatcaaga gacagctggt cgaaacaaga     2820

cagatcacaa agcacgtcgc acagatcctg gacagcagaa tgaacacaaa gtacgacgaa     2880

aacgacaagc tgatcagaga agtcaaggtc atcacactga agagcaagct ggtcagcgac     2940

ttcagaaagg acttccagtt ctacaaggtc agagaaatca acaactacca ccacgcacac     3000

gacgcatacc tgaacgcagt cgtcggaaca gcactgatca agaagtaccc gaagctggaa     3060

agcgaattcg tctacggaga ctacaaggtc tacgacgtca gaaagatgat cgcaaagagc     3120

gaacaggaaa tcggaaaggc aacagcaaag tacttcttct acagcaacat catgaacttc     3180

ttcaagacag aaatcacact ggcaaacgga gaaatcagaa agagaccgct gatcgaaaca     3240

aacggagaaa caggagaaat cgtctgggac aagggaagag acttcgcaac agtcagaaag     3300

gtcctgagca tgccgcaggt caacatcgtc aagaagacag aagtccagac aggaggattc     3360

agcaaggaaa gcatcctgcc gaagagaaac agcgacaagc tgatcgcaag aaagaaggac     3420

tgggacccga agaagtacgg aggattcgac agcccgacag tcgcatacag cgtcctggtc     3480

gtcgcaaagg tcgaaaaggg aaagagcaag aagctgaaga gcgtcaagga actgctggga     3540

atcacaatca tggaaagaag cagcttcgaa aagaacccga tcgacttcct ggaagcaaag     3600

ggatacaagg aagtcaagaa ggacctgatc atcaagctgc cgaagtacag cctgttcgaa     3660

ctggaaaacg gaagaaagag aatgctggca agcgcaggag aactgcagaa gggaaacgaa     3720

ctggcactgc cgagcaagta cgtcaacttc ctgtacctgg caagccacta cgaaaagctg     3780

aagggaagcc cggaagacaa cgaacagaag cagctgttcg tcgaacagca caagcactac     3840

ctggacgaaa tcatcgaaca gatcagcgaa ttcagcaaga gagtcatcct ggcagacgca     3900

aacctggaca aggtcctgag cgcatacaac aagcacagag acaagccgat cagagaacag     3960

gcagaaaaca tcatccacct gttcacactg acaaacctgg gagcaccggc agcattcaag     4020

tacttcgaca caacaatcga cagaaagaga tacacaagca caaaggaagt cctggacgca     4080

acactgatcc accagagcat cacaggactg tacgaaacaa gaatcgacct gagccagctg     4140

ggaggagacg gaggaggaag cccgaagaag aagagaaagg tcagcgaaag cgcaacaccg     4200

gaaagcgtca gcggatggag actgttcaag aagatcagct agctagccat cacatttaaa     4260

agcatctcag cctaccatga gaataagaga aagaaaatga agatcaatag cttattcatc     4320

tctttttctt tttcgttggt gtaaagccaa caccctgtct aaaaaacata aatttcttta     4380

atcattttgc ctcttttctc tgtgcttcaa ttaataaaaa atggaaagaa cctcgagaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaa                                                    4517


<210>  386
<211>  1381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence for Cas9 with NLS1

<400>  386

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Gly Gly  Gly Ser Leu Ala Ala  Lys Arg Ser Arg Thr  Thr 
    1370                 1375                 1380     


<210>  387
<211>  1381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence for Cas9 with NLS2

<400>  387

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Gly Gly  Gly Ser Gln Ala Ala  Lys Arg Ser Arg Thr  Thr 
    1370                 1375                 1380     


<210>  388
<211>  1382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence for Cas9 with NLS3

<400>  388

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Gly Gly  Gly Ser Pro Ala Pro  Ala Lys Arg Glu Arg  Thr Thr 
    1370                 1375                 1380         


<210>  389
<211>  1381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence for Cas9 with NLS4

<400>  389

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Gly Gly  Gly Ser Gln Ala Ala  Lys Arg Pro Arg Thr  Thr 
    1370                 1375                 1380     


<210>  390
<211>  1381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence for Cas9 with NLS5

<400>  390

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Gly Gly  Gly Ser Arg Ala Ala  Lys Arg Pro Arg Thr  Thr 
    1370                 1375                 1380     


<210>  391
<211>  1383
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence for Cas9 with NLS6

<400>  391

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Gly Gly  Gly Ser Ala Ala Ala  Lys Arg Ser Trp Ser  Met Ala Ala 
    1370                 1375                 1380             


<210>  392
<211>  1383
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence for Cas9 with NLS7

<400>  392

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Gly Gly  Gly Ser Ala Ala Ala  Lys Arg Val Trp Ser  Met Ala Phe 
    1370                 1375                 1380             


<210>  393
<211>  1383
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence for Cas9 with NLS8

<400>  393

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Gly Gly  Gly Ser Ala Ala Ala  Lys Arg Ser Trp Ser  Met Ala Phe 
    1370                 1375                 1380             


<210>  394
<211>  1382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence for Cas9 with NLS9

<400>  394

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Gly Gly  Gly Ser Ala Ala Ala  Lys Arg Lys Tyr Phe  Ala Ala 
    1370                 1375                 1380         


<210>  395
<211>  1382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence for Cas9 with NLS10

<400>  395

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Gly Gly  Gly Ser Arg Ala Ala  Lys Arg Lys Ala Phe  Ala Ala 
    1370                 1375                 1380         


<210>  396
<211>  1382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence for Cas9 with NLS11

<400>  396

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Gly Gly  Gly Ser Arg Ala Ala  Lys Arg Lys Tyr Phe  Ala Val 
    1370                 1375                 1380         


