                         SEQUENCE LISTING

<110>  INTELLIA THERAPEUTICS, INC.
 
<120>  POLYNUCLEOTIDES, COMPOSITIONS, AND METHODS FOR POLYPEPTIDE 
       EXPRESSION

<130>  01155-0027-00PCT

<150>  US 62/825,656
<151>  2019-03-28

<160>  204   

<170>  PatentIn version 3.5

<210>  1
<211>  1379
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 amino acid sequence

<400>  1

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Gly Gly  Gly Ser Pro Lys Lys  Lys Arg Lys Val 
    1370                 1375                 


<210>  2
<211>  4491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript with ORF having low U content

<400>  2
gggucccgca gucggcgucc agcggcucug cuuguucgug ugugugucgu ugcaggccuu       60

auucggaucc gccaccaugg acaagaagua cagcaucgga cuggacaucg gaacaaacag      120

cgucggaugg gcagucauca cagacgaaua caaggucccg agcaagaagu ucaagguccu      180

gggaaacaca gacagacaca gcaucaagaa gaaccugauc ggagcacugc uguucgacag      240

cggagaaaca gcagaagcaa caagacugaa gagaacagca agaagaagau acacaagaag      300

aaagaacaga aucugcuacc ugcaggaaau cuucagcaac gaaauggcaa aggucgacga      360

cagcuucuuc cacagacugg aagaaagcuu ccuggucgaa gaagacaaga agcacgaaag      420

acacccgauc uucggaaaca ucgucgacga agucgcauac cacgaaaagu acccgacaau      480

cuaccaccug agaaagaagc uggucgacag cacagacaag gcagaccuga gacugaucua      540

ccuggcacug gcacacauga ucaaguucag aggacacuuc cugaucgaag gagaccugaa      600

cccggacaac agcgacgucg acaagcuguu cauccagcug guccagacau acaaccagcu      660

guucgaagaa aacccgauca acgcaagcgg agucgacgca aaggcaaucc ugagcgcaag      720

acugagcaag agcagaagac uggaaaaccu gaucgcacag cugccgggag aaaagaagaa      780

cggacuguuc ggaaaccuga ucgcacugag ccugggacug acaccgaacu ucaagagcaa      840

cuucgaccug gcagaagacg caaagcugca gcugagcaag gacacauacg acgacgaccu      900

ggacaaccug cuggcacaga ucggagacca guacgcagac cuguuccugg cagcaaagaa      960

ccugagcgac gcaauccugc ugagcgacau ccugagaguc aacacagaaa ucacaaaggc     1020

accgcugagc gcaagcauga ucaagagaua cgacgaacac caccaggacc ugacacugcu     1080

gaaggcacug gucagacagc agcugccgga aaaguacaag gaaaucuucu ucgaccagag     1140

caagaacgga uacgcaggau acaucgacgg aggagcaagc caggaagaau ucuacaaguu     1200

caucaagccg auccuggaaa agauggacgg aacagaagaa cugcugguca agcugaacag     1260

agaagaccug cugagaaagc agagaacauu cgacaacgga agcaucccgc accagaucca     1320

ccugggagaa cugcacgcaa uccugagaag acaggaagac uucuacccgu uccugaagga     1380

caacagagaa aagaucgaaa agauccugac auucagaauc ccguacuacg ucggaccgcu     1440

ggcaagagga aacagcagau ucgcauggau gacaagaaag agcgaagaaa caaucacacc     1500

guggaacuuc gaagaagucg ucgacaaggg agcaagcgca cagagcuuca ucgaaagaau     1560

gacaaacuuc gacaagaacc ugccgaacga aaagguccug ccgaagcaca gccugcugua     1620

cgaauacuuc acagucuaca acgaacugac aaaggucaag uacgucacag aaggaaugag     1680

aaagccggca uuccugagcg gagaacagaa gaaggcaauc gucgaccugc uguucaagac     1740

aaacagaaag gucacaguca agcagcugaa ggaagacuac uucaagaaga ucgaaugcuu     1800

cgacagcguc gaaaucagcg gagucgaaga cagauucaac gcaagccugg gaacauacca     1860

cgaccugcug aagaucauca aggacaagga cuuccuggac aacgaagaaa acgaagacau     1920

ccuggaagac aucguccuga cacugacacu guucgaagac agagaaauga ucgaagaaag     1980

acugaagaca uacgcacacc uguucgacga caaggucaug aagcagcuga agagaagaag     2040

auacacagga uggggaagac ugagcagaaa gcugaucaac ggaaucagag acaagcagag     2100

cggaaagaca auccuggacu uccugaagag cgacggauuc gcaaacagaa acuucaugca     2160

gcugauccac gacgacagcc ugacauucaa ggaagacauc cagaaggcac aggucagcgg     2220

acagggagac agccugcacg aacacaucgc aaaccuggca ggaagcccgg caaucaagaa     2280

gggaauccug cagacaguca aggucgucga cgaacugguc aaggucaugg gaagacacaa     2340

gccggaaaac aucgucaucg aaauggcaag agaaaaccag acaacacaga agggacagaa     2400

gaacagcaga gaaagaauga agagaaucga agaaggaauc aaggaacugg gaagccagau     2460

ccugaaggaa cacccggucg aaaacacaca gcugcagaac gaaaagcugu accuguacua     2520

ccugcagaac ggaagagaca uguacgucga ccaggaacug gacaucaaca gacugagcga     2580

cuacgacguc gaccacaucg ucccgcagag cuuccugaag gacgacagca ucgacaacaa     2640

gguccugaca agaagcgaca agaacagagg aaagagcgac aacgucccga gcgaagaagu     2700

cgucaagaag augaagaacu acuggagaca gcugcugaac gcaaagcuga ucacacagag     2760

aaaguucgac aaccugacaa aggcagagag aggaggacug agcgaacugg acaaggcagg     2820

auucaucaag agacagcugg ucgaaacaag acagaucaca aagcacgucg cacagauccu     2880

ggacagcaga augaacacaa aguacgacga aaacgacaag cugaucagag aagucaaggu     2940

caucacacug aagagcaagc uggucagcga cuucagaaag gacuuccagu ucuacaaggu     3000

cagagaaauc aacaacuacc accacgcaca cgacgcauac cugaacgcag ucgucggaac     3060

agcacugauc aagaaguacc cgaagcugga aagcgaauuc gucuacggag acuacaaggu     3120

cuacgacguc agaaagauga ucgcaaagag cgaacaggaa aucggaaagg caacagcaaa     3180

guacuucuuc uacagcaaca ucaugaacuu cuucaagaca gaaaucacac uggcaaacgg     3240

agaaaucaga aagagaccgc ugaucgaaac aaacggagaa acaggagaaa ucgucuggga     3300

caagggaaga gacuucgcaa cagucagaaa gguccugagc augccgcagg ucaacaucgu     3360

caagaagaca gaaguccaga caggaggauu cagcaaggaa agcauccugc cgaagagaaa     3420

cagcgacaag cugaucgcaa gaaagaagga cugggacccg aagaaguacg gaggauucga     3480

cagcccgaca gucgcauaca gcguccuggu cgucgcaaag gucgaaaagg gaaagagcaa     3540

gaagcugaag agcgucaagg aacugcuggg aaucacaauc auggaaagaa gcagcuucga     3600

aaagaacccg aucgacuucc uggaagcaaa gggauacaag gaagucaaga aggaccugau     3660

caucaagcug ccgaaguaca gccuguucga acuggaaaac ggaagaaaga gaaugcuggc     3720

aagcgcagga gaacugcaga agggaaacga acuggcacug ccgagcaagu acgucaacuu     3780

ccuguaccug gcaagccacu acgaaaagcu gaagggaagc ccggaagaca acgaacagaa     3840

gcagcuguuc gucgaacagc acaagcacua ccuggacgaa aucaucgaac agaucagcga     3900

auucagcaag agagucaucc uggcagacgc aaaccuggac aagguccuga gcgcauacaa     3960

caagcacaga gacaagccga ucagagaaca ggcagaaaac aucauccacc uguucacacu     4020

gacaaaccug ggagcaccgg cagcauucaa guacuucgac acaacaaucg acagaaagag     4080

auacacaagc acaaaggaag uccuggacgc aacacugauc caccagagca ucacaggacu     4140

guacgaaaca agaaucgacc ugagccagcu gggaggagac ggaggaggaa gcccgaagaa     4200

gaagagaaag gucuagcuag ccaucacauu uaaaagcauc ucagccuacc augagaauaa     4260

gagaaagaaa augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag     4320

ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu     4380

ucaauuaaua aaaaauggaa agaaccucga gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a              4491


<210>  3
<211>  4405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript with ORF having low A content

<400>  3
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguacu       60

ccaucggccu ggacaucggc accaacuccg ugggcugggc cgugaucacc gacgaguaca      120

aggugcccuc caagaaguuc aaggugcugg gcaacaccga ccggcacucc aucaagaaga      180

accugaucgg cgcccugcug uucgacuccg gcgagaccgc cgaggccacc cggcugaagc      240

ggaccgcccg gcggcgguac acccggcgga agaaccggau cugcuaccug caggagaucu      300

ucuccaacga gauggccaag guggacgacu ccuucuucca ccggcuggag gaguccuucc      360

ugguggagga ggacaagaag cacgagcggc accccaucuu cggcaacauc guggacgagg      420

uggccuacca cgagaaguac cccaccaucu accaccugcg gaagaagcug guggacucca      480

ccgacaaggc cgaccugcgg cugaucuacc uggcccuggc ccacaugauc aaguuccggg      540

gccacuuccu gaucgagggc gaccugaacc ccgacaacuc cgacguggac aagcuguuca      600

uccagcuggu gcagaccuac aaccagcugu ucgaggagaa ccccaucaac gccuccggcg      660

uggacgccaa ggccauccug uccgcccggc uguccaaguc ccggcggcug gagaaccuga      720

ucgcccagcu gcccggcgag aagaagaacg gccuguucgg caaccugauc gcccuguccc      780

ugggccugac ccccaacuuc aaguccaacu ucgaccuggc cgaggacgcc aagcugcagc      840

uguccaagga caccuacgac gacgaccugg acaaccugcu ggcccagauc ggcgaccagu      900

acgccgaccu guuccuggcc gccaagaacc uguccgacgc cauccugcug uccgacaucc      960

ugcgggugaa caccgagauc accaaggccc cccuguccgc cuccaugauc aagcgguacg     1020

acgagcacca ccaggaccug acccugcuga aggcccuggu gcggcagcag cugcccgaga     1080

aguacaagga gaucuucuuc gaccagucca agaacggcua cgccggcuac aucgacggcg     1140

gcgccuccca ggaggaguuc uacaaguuca ucaagcccau ccuggagaag auggacggca     1200

ccgaggagcu gcuggugaag cugaaccggg aggaccugcu gcggaagcag cggaccuucg     1260

acaacggcuc caucccccac cagauccacc ugggcgagcu gcacgccauc cugcggcggc     1320

aggaggacuu cuaccccuuc cugaaggaca accgggagaa gaucgagaag auccugaccu     1380

uccggauccc cuacuacgug ggcccccugg cccggggcaa cucccgguuc gccuggauga     1440

cccggaaguc cgaggagacc aucacccccu ggaacuucga ggagguggug gacaagggcg     1500

ccuccgccca guccuucauc gagcggauga ccaacuucga caagaaccug cccaacgaga     1560

aggugcugcc caagcacucc cugcuguacg aguacuucac cguguacaac gagcugacca     1620

aggugaagua cgugaccgag ggcaugcgga agcccgccuu ccuguccggc gagcagaaga     1680

aggccaucgu ggaccugcug uucaagacca accggaaggu gaccgugaag cagcugaagg     1740

aggacuacuu caagaagauc gagugcuucg acuccgugga gaucuccggc guggaggacc     1800

gguucaacgc cucccugggc accuaccacg accugcugaa gaucaucaag gacaaggacu     1860

uccuggacaa cgaggagaac gaggacaucc uggaggacau cgugcugacc cugacccugu     1920

ucgaggaccg ggagaugauc gaggagcggc ugaagaccua cgcccaccug uucgacgaca     1980

aggugaugaa gcagcugaag cggcggcggu acaccggcug gggccggcug ucccggaagc     2040

ugaucaacgg cauccgggac aagcaguccg gcaagaccau ccuggacuuc cugaaguccg     2100

acggcuucgc caaccggaac uucaugcagc ugauccacga cgacucccug accuucaagg     2160

aggacaucca gaaggcccag guguccggcc agggcgacuc ccugcacgag cacaucgcca     2220

accuggccgg cucccccgcc aucaagaagg gcauccugca gaccgugaag gugguggacg     2280

agcuggugaa ggugaugggc cggcacaagc ccgagaacau cgugaucgag auggcccggg     2340

agaaccagac cacccagaag ggccagaaga acucccggga gcggaugaag cggaucgagg     2400

agggcaucaa ggagcugggc ucccagaucc ugaaggagca ccccguggag aacacccagc     2460

ugcagaacga gaagcuguac cuguacuacc ugcagaacgg ccgggacaug uacguggacc     2520

aggagcugga caucaaccgg cuguccgacu acgacgugga ccacaucgug ccccaguccu     2580

uccugaagga cgacuccauc gacaacaagg ugcugacccg guccgacaag aaccggggca     2640

aguccgacaa cgugcccucc gaggaggugg ugaagaagau gaagaacuac uggcggcagc     2700

ugcugaacgc caagcugauc acccagcgga aguucgacaa ccugaccaag gccgagcggg     2760

gcggccuguc cgagcuggac aaggccggcu ucaucaagcg gcagcuggug gagacccggc     2820

agaucaccaa gcacguggcc cagauccugg acucccggau gaacaccaag uacgacgaga     2880

acgacaagcu gauccgggag gugaagguga ucacccugaa guccaagcug guguccgacu     2940

uccggaagga cuuccaguuc uacaaggugc gggagaucaa caacuaccac cacgcccacg     3000

acgccuaccu gaacgccgug gugggcaccg cccugaucaa gaaguacccc aagcuggagu     3060

ccgaguucgu guacggcgac uacaaggugu acgacgugcg gaagaugauc gccaaguccg     3120

agcaggagau cggcaaggcc accgccaagu acuucuucua cuccaacauc augaacuucu     3180

ucaagaccga gaucacccug gccaacggcg agauccggaa gcggccccug aucgagacca     3240

acggcgagac cggcgagauc gugugggaca agggccggga cuucgccacc gugcggaagg     3300

ugcuguccau gccccaggug aacaucguga agaagaccga ggugcagacc ggcggcuucu     3360

ccaaggaguc cauccugccc aagcggaacu ccgacaagcu gaucgcccgg aagaaggacu     3420

gggaccccaa gaaguacggc ggcuucgacu cccccaccgu ggccuacucc gugcuggugg     3480

uggccaaggu ggagaagggc aaguccaaga agcugaaguc cgugaaggag cugcugggca     3540

ucaccaucau ggagcggucc uccuucgaga agaaccccau cgacuuccug gaggccaagg     3600

gcuacaagga ggugaagaag gaccugauca ucaagcugcc caaguacucc cuguucgagc     3660

uggagaacgg ccggaagcgg augcuggccu ccgccggcga gcugcagaag ggcaacgagc     3720

uggcccugcc cuccaaguac gugaacuucc uguaccuggc cucccacuac gagaagcuga     3780

agggcucccc cgaggacaac gagcagaagc agcuguucgu ggagcagcac aagcacuacc     3840

uggacgagau caucgagcag aucuccgagu ucuccaagcg ggugauccug gccgacgcca     3900

accuggacaa ggugcugucc gccuacaaca agcaccggga caagcccauc cgggagcagg     3960

ccgagaacau cauccaccug uucacccuga ccaaccuggg cgcccccgcc gccuucaagu     4020

acuucgacac caccaucgac cggaagcggu acaccuccac caaggaggug cuggacgcca     4080

cccugaucca ccaguccauc accggccugu acgagacccg gaucgaccug ucccagcugg     4140

gcggcgacgg cggcggcucc cccaagaaga agcggaaggu gugacuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucuc     4320

ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaa                                           4405


<210>  4
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Guide RNA G000502


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  4
acacaaauac caguccagcg guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  5
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: E-single enriched Cas9 ORF

<400>  5
auggacaaga aguacagcau cggccuggac aucggcacga acagcguggg cugggccgug       60

aucacggacg aguacaaggu gcccagcaag aaguucaagg ugcugggcaa cacggaccgg      120

cacagcauca agaagaaccu gaucggcgcc cugcuguucg acagcggcga gacggccgag      180

gccacgcggc ugaagcggac ggcccggcgg cgguacacgc ggcggaagaa ccggaucugc      240

uaccugcagg agaucuucag caacgagaug gccaaggugg acgacagcuu cuuccaccgg      300

cuggaggaga gcuuccuggu ggaggaggac aagaagcacg agcggcaccc caucuucggc      360

aacaucgugg acgagguggc cuaccacgag aaguacccca cgaucuacca ccugcggaag      420

aagcuggugg acagcacgga caaggccgac cugcggcuga ucuaccuggc ccuggcccac      480

augaucaagu uccggggcca cuuccugauc gagggcgacc ugaaccccga caacagcgac      540

guggacaagc uguucaucca gcuggugcag acguacaacc agcuguucga ggagaacccc      600

aucaacgcca gcggcgugga cgccaaggcc auccugagcg cccggcugag caagagccgg      660

cggcuggaga accugaucgc ccagcugccc ggcgagaaga agaacggccu guucggcaac      720

cugaucgccc ugagccuggg ccugacgccc aacuucaaga gcaacuucga ccuggccgag      780

gacgccaagc ugcagcugag caaggacacg uacgacgacg accuggacaa ccugcuggcc      840

cagaucggcg accaguacgc cgaccuguuc cuggccgcca agaaccugag cgacgccauc      900

cugcugagcg acauccugcg ggugaacacg gagaucacga aggccccccu gagcgccagc      960

augaucaagc gguacgacga gcaccaccag gaccugacgc ugcugaaggc ccuggugcgg     1020

cagcagcugc ccgagaagua caaggagauc uucuucgacc agagcaagaa cggcuacgcc     1080

ggcuacaucg acggcggcgc cagccaggag gaguucuaca aguucaucaa gcccauccug     1140

gagaagaugg acggcacgga ggagcugcug gugaagcuga accgggagga ccugcugcgg     1200

aagcagcgga cguucgacaa cggcagcauc ccccaccaga uccaccuggg cgagcugcac     1260

gccauccugc ggcggcagga ggacuucuac cccuuccuga aggacaaccg ggagaagauc     1320

gagaagaucc ugacguuccg gauccccuac uacgugggcc cccuggcccg gggcaacagc     1380

cgguucgccu ggaugacgcg gaagagcgag gagacgauca cgcccuggaa cuucgaggag     1440

gugguggaca agggcgccag cgcccagagc uucaucgagc ggaugacgaa cuucgacaag     1500

aaccugccca acgagaaggu gcugcccaag cacagccugc uguacgagua cuucacggug     1560

uacaacgagc ugacgaaggu gaaguacgug acggagggca ugcggaagcc cgccuuccug     1620

agcggcgagc agaagaaggc caucguggac cugcuguuca agacgaaccg gaaggugacg     1680

gugaagcagc ugaaggagga cuacuucaag aagaucgagu gcuucgacag cguggagauc     1740

agcggcgugg aggaccgguu caacgccagc cugggcacgu accacgaccu gcugaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccugga ggacaucgug     1860

cugacgcuga cgcuguucga ggaccgggag augaucgagg agcggcugaa gacguacgcc     1920

caccuguucg acgacaaggu gaugaagcag cugaagcggc ggcgguacac gggcuggggc     1980

cggcugagcc ggaagcugau caacggcauc cgggacaagc agagcggcaa gacgauccug     2040

gacuuccuga agagcgacgg cuucgccaac cggaacuuca ugcagcugau ccacgacgac     2100

agccugacgu ucaaggagga cauccagaag gcccagguga gcggccaggg cgacagccug     2160

cacgagcaca ucgccaaccu ggccggcagc cccgccauca agaagggcau ccugcagacg     2220

gugaaggugg uggacgagcu ggugaaggug augggccggc acaagcccga gaacaucgug     2280

aucgagaugg cccgggagaa ccagacgacg cagaagggcc agaagaacag ccgggagcgg     2340

augaagcgga ucgaggaggg caucaaggag cugggcagcc agauccugaa ggagcacccc     2400

guggagaaca cgcagcugca gaacgagaag cuguaccugu acuaccugca gaacggccgg     2460

gacauguacg uggaccagga gcuggacauc aaccggcuga gcgacuacga cguggaccac     2520

aucgugcccc agagcuuccu gaaggacgac agcaucgaca acaaggugcu gacgcggagc     2580

gacaagaacc ggggcaagag cgacaacgug cccagcgagg agguggugaa gaagaugaag     2640

aacuacuggc ggcagcugcu gaacgccaag cugaucacgc agcggaaguu cgacaaccug     2700

acgaaggccg agcggggcgg ccugagcgag cuggacaagg ccggcuucau caagcggcag     2760

cugguggaga cgcggcagau cacgaagcac guggcccaga uccuggacag ccggaugaac     2820

acgaaguacg acgagaacga caagcugauc cgggagguga aggugaucac gcugaagagc     2880

aagcugguga gcgacuuccg gaaggacuuc caguucuaca aggugcggga gaucaacaac     2940

uaccaccacg cccacgacgc cuaccugaac gccguggugg gcacggcccu gaucaagaag     3000

uaccccaagc uggagagcga guucguguac ggcgacuaca agguguacga cgugcggaag     3060

augaucgcca agagcgagca ggagaucggc aaggccacgg ccaaguacuu cuucuacagc     3120

aacaucauga acuucuucaa gacggagauc acgcuggcca acggcgagau ccggaagcgg     3180

ccccugaucg agacgaacgg cgagacgggc gagaucgugu gggacaaggg ccgggacuuc     3240

gccacggugc ggaaggugcu gagcaugccc caggugaaca ucgugaagaa gacggaggug     3300

cagacgggcg gcuucagcaa ggagagcauc cugcccaagc ggaacagcga caagcugauc     3360

gcccggaaga aggacuggga ccccaagaag uacggcggcu ucgacagccc cacgguggcc     3420

uacagcgugc uggugguggc caagguggag aagggcaaga gcaagaagcu gaagagcgug     3480

aaggagcugc ugggcaucac gaucauggag cggagcagcu ucgagaagaa ccccaucgac     3540

uuccuggagg ccaagggcua caaggaggug aagaaggacc ugaucaucaa gcugcccaag     3600

uacagccugu ucgagcugga gaacggccgg aagcggaugc uggccagcgc cggcgagcug     3660

cagaagggca acgagcuggc ccugcccagc aaguacguga acuuccugua ccuggccagc     3720

cacuacgaga agcugaaggg cagccccgag gacaacgagc agaagcagcu guucguggag     3780

cagcacaagc acuaccugga cgagaucauc gagcagauca gcgaguucag caagcgggug     3840

auccuggccg acgccaaccu ggacaaggug cugagcgccu acaacaagca ccgggacaag     3900

cccauccggg agcaggccga gaacaucauc caccuguuca cgcugacgaa ccugggcgcc     3960

cccgccgccu ucaaguacuu cgacacgacg aucgaccgga agcgguacac gagcacgaag     4020

gaggugcugg acgccacgcu gauccaccag agcaucacgg gccuguacga gacgcggauc     4080

gaccugagcc agcugggcgg cgacggcggc ggcagcccca agaagaagcg gaagguguag     4140


<210>  6
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: E-pair enriched, I-pair depleted Cas9 ORF

<400>  6
auggacaaga aguacagcau cggccuggac aucggcacga acagcguugg cugggcugug       60

aucacggacg aguacaaggu ucccagcaag aaguucaagg ugcugggcaa cacggaccgg      120

cacagcauca agaagaaucu gaucggugca cugcuguucg acagcgguga gacggccgaa      180

gccacgcggc ugaagcggac ggcccggcgg cgguacacgc ggcggaagaa ccggaucugc      240

uaccugcagg agaucuucag caacgagaug gccaaggugg acgacagcuu cuuccaccgg      300

cuggaggaga gcuuccuggu ggaggaggac aagaagcacg agcggcaccc caucuucggc      360

aacaucgugg acgaaguggc cuaccacgag aaguacccca cgaucuacca ccugcggaag      420

aagcuggugg acucgacgga caaggccgac cugcggcuga ucuaccuggc acuggcccac      480

augaucaagu uccggggcca cuuccugauc gagggcgacc ugaacccuga caacagcgac      540

guggacaagc uguucaucca gcuggugcag accuacaacc agcuguucga ggagaacccc      600

aucaacgcca gcggcgugga cgccaaggcc auccucagcg cccggcucag caagagccgg      660

cggcuggaga aucugaucgc ccagcuuccc ggugagaaga agaauggccu guucggcaau      720

cugaucgcac ucagccuggg ccugacuccc aacuucaaga gcaacuucga ccuggccgag      780

gacgccaagc ugcagcucag caaggacacc uacgacgacg accuggacaa ucugcuggcc      840

cagaucggcg accaguacgc cgaccuguuc cuggcugcca agaaucucag cgacgccauc      900

cugcucagcg acauccugcg ggugaacaca gagaucacga aggccccccu cagcgccagc      960

augaucaagc gguacgacga gcaccaccag gaccugacgc ugcugaaggc acuggugcgg     1020

cagcagcuuc cagagaagua caaggagauc uucuucgacc agagcaagaa uggcuacgcc     1080

ggcuacaucg acgguggugc cagccaggag gaguucuaca aguucaucaa gcccauccug     1140

gagaagaugg acggcacaga ggagcugcug gugaagcuga acagggagga ccugcugcgg     1200

aagcagcgga cguucgacaa uggcagcauc ccccaccaga uccaccuggg ugagcugcac     1260

gccauccugc ggcggcagga ggacuucuac cccuuccuga aggacaacag ggagaagauc     1320

gagaagaucc ugacguuccg gauccccuac uacguuggcc cccuggcccg gggcaacagc     1380

cgguucgccu ggaugacgcg gaagagcgag gagacgauca cucccuggaa cuucgaggaa     1440

gugguggaca agggugccag cgcccagagc uucaucgagc ggaugacgaa cuucgacaag     1500

aaucuuccca acgagaaggu gcuucccaag cacagccugc uguacgagua cuucacggug     1560

uacaacgagc ugacgaaggu gaaguacgug acagagggca ugcggaagcc cgccuuccuc     1620

agcggugagc agaagaaggc caucguggac cugcuguuca agacgaaccg gaaggugacg     1680

gugaagcagc ugaaggagga cuacuucaag aagaucgagu gcuucgacag cguggagauc     1740

agcggcgugg aggaccgguu caacgccagc cugggcaccu accacgaccu gcugaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccugga ggacaucgug     1860

cugacgcuga cgcuguucga ggacagggag augaucgagg agcggcugaa gaccuacgcc     1920

caccuguucg acgacaaggu gaugaagcag cugaagcggc ggcgguacac gggcuggggc     1980

cggcucagcc ggaagcugau caauggcauc cgagacaagc agagcggcaa gacgauccug     2040

gacuuccuga agagcgacgg cuucgccaac cggaacuuca ugcagcugau ccacgacgac     2100

agccugacgu ucaaggagga cauccagaag gcccagguca gcggccaggg cgacagccug     2160

cacgagcaca ucgccaaucu ggccggcagc cccgccauca agaagggcau ccugcagacg     2220

gugaaggugg uggacgagcu ggugaaggug augggccggc acaagccaga gaacaucgug     2280

aucgagaugg ccagggagaa ccagacgacu cagaagggcc agaagaacag cagggagcgg     2340

augaagcgga ucgaggaggg caucaaggag cugggcagcc agauccugaa ggagcacccc     2400

guggagaaca cucagcugca gaacgagaag cuguaccugu acuaccugca gaauggccga     2460

gacauguacg uggaccagga gcuggacauc aaccggcuca gcgacuacga cguggaccac     2520

aucguucccc agagcuuccu gaaggacgac agcaucgaca acaaggugcu gacgcggagc     2580

gacaagaacc ggggcaagag cgacaacguu cccagcgagg aaguggugaa gaagaugaag     2640

aacuacuggc ggcagcugcu gaacgccaag cugaucacuc agcggaaguu cgacaaucug     2700

acgaaggccg agcggggugg ccucagcgag cuggacaagg ccggcuucau caagcggcag     2760

cugguggaga cgcggcagau cacgaagcac guggcccaga uccuggacag ccggaugaac     2820

acgaaguacg acgagaacga caagcugauc agggaaguga aggugaucac gcugaagagc     2880

aagcugguca gcgacuuccg gaaggacuuc caguucuaca aggugaggga gaucaacaac     2940

uaccaccacg cccacgacgc cuaccugaac gcugugguug gcacggcacu gaucaagaag     3000

uaccccaagc uggagagcga guucguguac ggcgacuaca agguguacga cgugcggaag     3060

augaucgcca agagcgagca ggagaucggc aaggccacgg ccaaguacuu cuucuacagc     3120

aacaucauga acuucuucaa gacagagauc acgcuggcca auggugagau ccggaagcgg     3180

ccccugaucg agacgaaugg ugagacgggu gagaucgugu gggacaaggg ccgagacuuc     3240

gccacggugc ggaaggugcu cagcaugccc caggugaaca ucgugaagaa gacagaagug     3300

cagacgggug gcuucagcaa ggagagcauc cuucccaagc ggaacagcga caagcugauc     3360

gcccggaaga aggacuggga ccccaagaag uacgguggcu ucgacagccc cacgguggcc     3420

uacagcgugc uggugguggc caagguggag aagggcaaga gcaagaagcu gaagagcgug     3480

aaggagcugc ugggcaucac gaucauggag cggagcagcu ucgagaagaa ccccaucgac     3540

uuccuggaag ccaagggcua caaggaagug aagaaggacc ugaucaucaa gcuucccaag     3600

uacagccugu ucgagcugga gaauggccgg aagcggaugc uggccagcgc cggugagcug     3660

cagaagggca acgagcuggc acuucccagc aaguacguga acuuccugua ccuggccagc     3720

cacuacgaga agcugaaggg cagcccagag gacaacgagc agaagcagcu guucguggag     3780

cagcacaagc acuaccugga cgagaucauc gagcagauca gcgaguucag caagcgggug     3840

auccuggccg acgccaaucu ggacaaggug cucagcgccu acaacaagca ccgagacaag     3900

cccaucaggg agcaggccga gaacaucauc caccuguuca cgcugacgaa ucugggugcc     3960

cccgcugccu ucaaguacuu cgacacgacg aucgaccgga agcgguacac gucgacgaag     4020

gaagugcugg acgccacgcu gauccaccag agcaucacgg gccuguacga gacgcggauc     4080

gaccucagcc agcugggugg cgacgguggu ggcagcccca agaagaagcg gaagguguag     4140


<210>  7
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: E-pair & E-single enriched, I-pair & I-single depleted
       Cas9 ORF

<400>  7
auggacaaga aguacagcau cggccucgac aucggcacca acagcguugg cugggcugug       60

aucaccgacg aguacaaggu ucccucaaag aaguucaagg uccucggcaa caccgaccgc      120

cacagcauca agaagaaucu caucggugca cugcucuucg acagcgguga gaccgcggaa      180

gccacccgcc ucaagcggac cgcccgccgc cgcuacaccc gccgcaagaa ccgcaucugc      240

uaccuccagg agaucuucag caacgagaug gccaaggucg acgacagcuu cuuccaccgc      300

cucgaggaga gcuuccuggu cgaggaggac aagaagcacg agcgccaccc caucuucggc      360

aacaucgucg acgaagucgc cuaccacgag aaguacccca ccaucuacca ccugcggaag      420

aagcucgucg acucgacuga caaggccgac cugcggcuca ucuaccucgc acuggcccac      480

augauaaagu uccgcggcca cuuccugauc gagggcgacc ucaacccuga caacagcgac      540

gucgacaagc ucuucaucca gcucguccag accuacaacc agcucuucga ggagaacccc      600

aucaacgcca gcggcgucga cgccaaggcc auccucagcg cccgccucag caagagccgc      660

cgccucgaga aucucaucgc ccagcuucca ggugagaaga agaaugggcu cuucggcaau      720

cucaucgcac ucagccucgg ccucacuccc aacuucaaga gcaacuucga ccucgcggag      780

gacgccaagc uccagcucag caaggacacc uacgacgacg accucgacaa ucuccucgcc      840

cagaucggcg accaguacgc cgaccucuuc cuggcugcca agaaucucag cgacgccauc      900

cuccucagcg acauccugcg ggucaacaca gagaucacca aggccccccu cagcgccagc      960

augauaaagc gcuacgacga gcaccaccag gaccucaccc uccucaaggc acugguccgc     1020

cagcagcuuc cagagaagua caaggagauc uucuucgacc agagcaagaa uggguacgcc     1080

ggguacaucg acgguggugc cagccaggag gaguucuaca aguucaucaa gcccauccuc     1140

gagaagaugg acggcacaga ggagcugcuc gucaagcuca acagggagga ccuccugcgg     1200

aagcagcgga ccuucgacaa ugggagcauc ccccaccaga uccaccucgg ugagcugcac     1260

gccauccugc ggcgccagga ggacuucuac cccuuccuga aggacaacag ggagaagauc     1320

gagaagaucc ucaccuuccg cauccccuac uacguuggcc cccucgcccg cggcaacagc     1380

cgcuucgccu ggaugacccg caagagcgag gagaccauca cucccuggaa cuucgaggaa     1440

gucgucgaca agggugccag cgcccagagc uucaucgagc gcaugaccaa cuucgacaag     1500

aaucuuccaa acgagaaggu ccuuccaaag cacagccucc ucuacgagua cuucaccguc     1560

uacaacgagc ugaccaaggu caaguacguc acagagggca ugcgcaagcc agccuuccuc     1620

agcggugagc agaagaaggc caucgucgac cuccucuuca agaccaaccg caaggucacc     1680

gucaagcagc ucaaggagga cuacuucaag aagaucgagu gcuucgacag cgucgagauc     1740

agcggcgucg aggaccgcuu caacgccagc cucggcaccu accacgaccu ccucaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccucga ggacaucguc     1860

cucacccuca cccucuucga ggacagggag augauagagg agcgccucaa gaccuacgcc     1920

caccucuucg acgacaaggu caugaagcag cucaagcgcc gccgcuacac cggcuggggc     1980

cgccucagcc gcaagcucau caaugggauc cgagacaagc agagcggcaa gaccauccuc     2040

gacuuccuga agagcgacgg cuucgccaac cgcaacuuca ugcagcucau ccacgacgac     2100

agccucaccu ucaaggagga cauccagaag gcccagguca gcggccaggg cgacagccuc     2160

cacgagcaca ucgccaaucu cgccgggagc ccagccauca agaaggggau ccuccagacc     2220

gucaaggucg ucgacgagcu ggucaagguc augggccgcc acaagccaga gaacaucguc     2280

aucgagaugg ccagggagaa ccagaccacu caaaaggggc agaagaacag cagggagcgc     2340

augaagcgca ucgaggaggg caucaaggag cugggcagcc agauccucaa ggagcacccc     2400

gucgagaaca cucaacucca gaacgagaag cucuaccucu acuaccucca gaaugggcga     2460

gacauguacg ucgaccagga gcuggacauc aaccgccuca gcgacuacga cgucgaccac     2520

aucguucccc agagcuuccu gaaggacgac agcaucgaca acaagguccu cacccgaagc     2580

gacaagaacc gcggcaagag cgacaacguu cccucagagg aagucgucaa gaagaugaag     2640

aacuacuggc gccagcuccu caacgccaag cucaucacuc aacgcaaguu cgacaaucuc     2700

accaaggcgg agcgcggugg ccucagcgag cuggacaagg ccggguucau caagcgccag     2760

cucgucgaga cccgccagau caccaagcac gucgcccaga uccucgacag ccgcaugaac     2820

accaaguacg acgagaacga caagcucauc agggaaguca aggucaucac ccucaagagc     2880

aagcucguca gcgacuuccg caaggacuuc caguucuaca aggucaggga gaucaacaac     2940

uaccaccacg cccacgacgc cuaccucaac gcugugguug gcaccgcacu gaucaagaag     3000

uaccccaagc ucgagagcga guucgucuac ggcgacuaca aggucuacga cguccgcaag     3060

augauagcca agagcgagca ggagaucggc aaggccaccg ccaaguacuu cuucuacagc     3120

aacaucauga acuucuucaa gacagagauc acccucgcca auggugagau ccgcaagcgc     3180

ccccucaucg agaccaaugg ugagaccggu gagaucgucu gggacaaggg gcgagacuuc     3240

gccaccgucc gcaagguccu cagcaugccc caggugaaca ucgucaagaa gacagaaguc     3300

cagaccggug gcuucagcaa ggagagcauc cuuccaaagc gcaacagcga caagcucauc     3360

gcccgcaaga aggacuggga ccccaagaag uacgguggcu ucgacagccc caccgucgcc     3420

uacagcgucc ucgucgucgc caaggucgag aaggggaaga gcaagaagcu caagagcguc     3480

aaggagcugc ucggcaucac caucauggag cgaagcagcu ucgagaagaa ccccaucgac     3540

uuccuggaag ccaaggggua caaggaaguc aagaaggacc ucaucaucaa gcuuccaaag     3600

uacagccucu ucgagcugga gaaugggcgc aagcgcaugc ucgccagcgc cggugagcug     3660

cagaagggga acgagcuggc acuucccuca aaguacguca acuuccugua ccucgccagc     3720

cacuacgaga agcucaaggg gagcccagag gacaacgagc agaagcagcu cuucgucgag     3780

cagcacaagc acuaccucga cgagaucauc gagcagauca gcgaguucag caagcgcguc     3840

auccucgccg acgccaaucu cgacaagguc cucagcgccu acaacaagca ccgagacaag     3900

cccaucaggg agcaggccga gaacaucauc caccucuuca cccucaccaa ucucggugcc     3960

ccagcugccu ucaaguacuu cgacaccacc aucgaccgca agcgcuacac cucgacuaag     4020

gaaguccucg acgccacccu cauccaccag agcaucaccg gccucuacga gacccgcauc     4080

gaccucagcc agcucggugg cgacgguggu ggcagcccca agaagaagcg caaggucuag     4140


<210>  8
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: I-pair depleted and/or I-single depleted Cas9 ORF

<400>  8
auggacaaga aguacagcau cggccuggac aucggcacga acagcguugg cugggcugug       60

aucacggacg aguacaaggu ucccucaaag aaguucaagg ugcugggcaa cacggaccgg      120

cacagcauca agaagaaucu caucggugca cugcuguucg acagcgguga gacggccgaa      180

gccacgcggc ugaagcggac ggcccgccgg cgguacacgc ggcggaagaa ccggaucugc      240

uaccugcagg agaucuucag caacgagaug gccaaggugg acgacagcuu cuuccaccgg      300

cuggaggaga gcuuccuggu ggaggaggac aagaagcacg agcggcaccc caucuucggc      360

aacaucgugg acgaagucgc cuaccacgag aaguacccca ccaucuacca ccugcggaag      420

aagcuggugg acucgacuga caaggccgac cugcggcuga ucuaccuggc acuggcccac      480

augauaaagu uccggggcca cuuccugauc gagggcgacc ugaacccuga caacagcgac      540

guggacaagc uguucaucca gcuggugcag accuacaacc agcuguucga ggagaacccc      600

aucaacgcca gcggcgugga cgccaaggcc auccucagcg cccgccucag caagagccgg      660

cggcuggaga aucucaucgc ccagcuucca ggugagaaga agaaugggcu guucggcaau      720

cucaucgcac ucagccuggg ccugacuccc aacuucaaga gcaacuucga ccuggccgag      780

gacgccaagc ugcagcucag caaggacacc uacgacgacg accuggacaa ucuccuggcc      840

cagaucggcg accaguacgc cgaccuguuc cuggcugcca agaaucucag cgacgccauc      900

cugcucagcg acauccugcg ggugaacaca gagaucacga aggccccccu cagcgccagc      960

augauaaagc gguacgacga gcaccaccag gaccugacgc ugcugaaggc acuggugcgg     1020

cagcagcuuc cagagaagua caaggagauc uucuucgacc agagcaagaa uggguacgcc     1080

ggguacaucg acgguggugc cagccaggag gaguucuaca aguucaucaa gcccauccug     1140

gagaagaugg acggcacaga ggagcugcug gugaagcuga acagggagga ccugcugcgg     1200

aagcagcgga cguucgacaa ugggagcauc ccccaccaga uccaccuggg ugagcugcac     1260

gccauccugc ggcggcagga ggacuucuac cccuuccuga aggacaacag ggagaagauc     1320

gagaagaucc ugacguuccg gauccccuac uacguuggcc cccuggcccg cggcaacagc     1380

cgguucgccu ggaugacgcg gaagagcgag gagacgauca cucccuggaa cuucgaggaa     1440

gucguggaca agggugccag cgcccagagc uucaucgagc ggaugacgaa cuucgacaag     1500

aaucuuccaa acgagaaggu gcuuccaaag cacagccugc uguacgagua cuucacggug     1560

uacaacgagc ugacgaaggu gaaguacgug acagagggca ugcggaagcc cgccuuccuc     1620

agcggugagc agaagaaggc caucguggac cugcuguuca agacgaaccg gaaggugacg     1680

gugaagcagc ugaaggagga cuacuucaag aagaucgagu gcuucgacag cguggagauc     1740

agcggcgugg aggaccgguu caacgccagc cugggcaccu accacgaccu gcugaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccugga ggacaucgug     1860

cugacgcuga cgcuguucga ggacagggag augauagagg agcggcugaa gaccuacgcc     1920

caccuguucg acgacaaggu gaugaagcag cugaagcggc ggcgguacac gggcuggggc     1980

cggcucagcc ggaagcugau caaugggauc cgagacaagc agagcggcaa gacgauccug     2040

gacuuccuga agagcgacgg cuucgccaac cggaacuuca ugcagcugau ccacgacgac     2100

agccugacgu ucaaggagga cauccagaag gcccagguca gcggccaggg cgacagccug     2160

cacgagcaca ucgccaaucu cgccgggagc cccgccauca agaaggggau ccugcagacg     2220

gugaaggugg uggacgagcu ggugaaggug augggccggc acaagccaga gaacaucgug     2280

aucgagaugg ccagggagaa ccagacgacu caaaaggggc agaagaacag cagggagcgg     2340

augaagcgga ucgaggaggg caucaaggag cugggcagcc agauccugaa ggagcacccc     2400

guggagaaca cucaacugca gaacgagaag cuguaccugu acuaccugca gaaugggcga     2460

gacauguacg uggaccagga gcuggacauc aaccggcuca gcgacuacga cguggaccac     2520

aucguucccc agagcuuccu gaaggacgac agcaucgaca acaaggugcu gacgcggagc     2580

gacaagaacc ggggcaagag cgacaacguu cccucagagg aagucgugaa gaagaugaag     2640

aacuacuggc ggcagcugcu gaacgccaag cugaucacuc aacggaaguu cgacaaucuc     2700

acgaaggccg agcggggugg ccucagcgag cuggacaagg ccggguucau caagcggcag     2760

cugguggaga cgcggcagau cacgaagcac guggcccaga uccuggacag ccggaugaac     2820

acgaaguacg acgagaacga caagcugauc agggaaguca aggugaucac gcugaagagc     2880

aagcugguca gcgacuuccg gaaggacuuc caguucuaca aggugaggga gaucaacaac     2940

uaccaccacg cccacgacgc cuaccugaac gcugugguug gcacggcacu gaucaagaag     3000

uaccccaagc uggagagcga guucguguac ggcgacuaca agguguacga cgugcggaag     3060

augauagcca agagcgagca ggagaucggc aaggccacgg ccaaguacuu cuucuacagc     3120

aacaucauga acuucuucaa gacagagauc acgcuggcca auggugagau ccggaagcgg     3180

ccccugaucg agacgaaugg ugagacgggu gagaucgugu gggacaaggg gcgagacuuc     3240

gccacggugc ggaaggugcu cagcaugccc caggugaaca ucgugaagaa gacagaaguc     3300

cagacgggug gcuucagcaa ggagagcauc cuuccaaagc ggaacagcga caagcugauc     3360

gcccgcaaga aggacuggga ccccaagaag uacgguggcu ucgacagccc caccguggcc     3420

uacagcgugc uggugguggc caagguggag aaggggaaga gcaagaagcu gaagagcgug     3480

aaggagcugc ugggcaucac gaucauggag cggagcagcu ucgagaagaa ccccaucgac     3540

uuccuggaag ccaaggggua caaggaaguc aagaaggacc ugaucaucaa gcuuccaaag     3600

uacagccugu ucgagcugga gaaugggcgg aagcggaugc uggccagcgc cggugagcug     3660

cagaagggga acgagcuggc acuucccuca aaguacguga acuuccugua ccuggccagc     3720

cacuacgaga agcugaaggg gagcccagag gacaacgagc agaagcagcu guucguggag     3780

cagcacaagc acuaccugga cgagaucauc gagcagauca gcgaguucag caagcgggug     3840

auccuggccg acgccaaucu cgacaaggug cucagcgccu acaacaagca ccgagacaag     3900

cccaucaggg agcaggccga gaacaucauc caccuguuca cgcugacgaa ucucggugcc     3960

cccgcugccu ucaaguacuu cgacacgacg aucgaccgga agcgguacac gucgacuaag     4020

gaaguccugg acgccacgcu gauccaccag agcaucacgg gccuguacga gacgcggauc     4080

gaccucagcc agcugggugg cgacgguggu ggcagcccca agaagaagcg gaagguguag     4140


<210>  9
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: E-Pair enriched Cas9 ORF

<400>  9
auggacaaga aguacagcau cggccucgac aucggcacca acagcguugg cugggcugug       60

aucaccgacg aguacaaggu ucccucaaag aaguucaagg uccucggcaa caccgaccgc      120

cacagcauca agaagaaucu caucggugca cugcucuucg acagcgguga gaccgcggaa      180

gccacccgcc ucaagcgcac cgcccgccgc cgcuacaccc gccgcaagaa ccgcaucugc      240

uaccuccagg agaucuucag caacgagaug gccaaggucg acgacagcuu cuuccaccgc      300

cucgaggaga gcuuccuggu cgaggaggac aagaagcacg agcgccaccc caucuucggc      360

aacaucgucg acgaagucgc cuaccacgag aaguacccca ccaucuacca ccugcggaag      420

aagcucgucg acucgacuga caaggccgac cugcggcuca ucuaccucgc acuggcccac      480

augauaaagu uccgcggcca cuuccugauc gagggcgacc ucaacccuga caacagcgac      540

gucgacaagc ucuucaucca gcucguccag accuacaacc agcucuucga ggagaacccc      600

aucaacgcca gcggcgucga cgccaaggcc auccucagcg cccgccucag caagagccgc      660

cgccucgaga aucucaucgc ccagcuucca ggugagaaga agaaugggcu cuucggcaau      720

cucaucgcac ucagccucgg ccucacuccc aacuucaaga gcaacuucga ccucgcggag      780

gacgccaagc uccagcucag caaggacacc uacgacgacg accucgacaa ucuccucgcc      840

cagaucggcg accaguacgc cgaccucuuc cuggcugcca agaaucucag cgacgccauc      900

cuccucagcg acauccugcg ggucaacaca gagaucacca aggccccccu cagcgccagc      960

augauaaagc gcuacgacga gcaccaccag gaccucaccc uccucaaggc acugguccgc     1020

cagcagcuuc cagagaagua caaggagauc uucuucgacc agagcaagaa uggguacgcc     1080

ggguacaucg acgguggugc cagccaggag gaguucuaca aguucaucaa gcccauccuc     1140

gagaagaugg acggcacaga ggagcugcuc gucaagcuca acagggagga ccuccugcgg     1200

aagcagcgca ccuucgacaa ugggagcauc ccccaccaga uccaccucgg ugagcugcac     1260

gccauccugc ggcgccagga ggacuucuac cccuuccuga aggacaacag ggagaagauc     1320

gagaagaucc ucaccuuccg cauccccuac uacguuggcc cccucgcccg cggcaacagc     1380

cgcuucgccu ggaugacccg caagagcgag gagaccauca cucccuggaa cuucgaggaa     1440

gucgucgaca agggugccag cgcccagagc uucaucgagc gcaugaccaa cuucgacaag     1500

aaucuuccaa acgagaaggu ccuuccaaag cacagccucc ucuacgagua cuucaccguc     1560

uacaacgagc ugaccaaggu caaguacguc acagagggca ugcgcaagcc agccuuccuc     1620

agcggugagc agaagaaggc caucgucgac cuccucuuca agaccaaccg caaggucacc     1680

gucaagcagc ucaaggagga cuacuucaag aagaucgagu gcuucgacag cgucgagauc     1740

agcggcgucg aggaccgcuu caacgccagc cucggcaccu accacgaccu ccucaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccucga ggacaucguc     1860

cucacccuca cccucuucga ggacagggag augauagagg agcgccucaa gaccuacgcc     1920

caccucuucg acgacaaggu caugaagcag cucaagcgcc gccgcuacac cggcuggggc     1980

cgccucagcc gcaagcucau caaugggauc cgagacaagc agagcggcaa gaccauccuc     2040

gacuuccuga agagcgacgg cuucgccaac cgcaacuuca ugcagcucau ccacgacgac     2100

agccucaccu ucaaggagga cauccagaag gcccagguca gcggccaggg cgacagccuc     2160

cacgagcaca ucgccaaucu cgccgggagc ccagccauca agaaggggau ccuccagacc     2220

gucaaggucg ucgacgagcu ggucaagguc augggccgcc acaagccaga gaacaucguc     2280

aucgagaugg ccagggagaa ccagaccacu caaaaggggc agaagaacag cagggagcgc     2340

augaagcgca ucgaggaggg caucaaggag cugggcagcc agauccucaa ggagcacccc     2400

gucgagaaca cucaacucca gaacgagaag cucuaccucu acuaccucca gaaugggcga     2460

gacauguacg ucgaccagga gcuggacauc aaccgccuca gcgacuacga cgucgaccac     2520

aucguucccc agagcuuccu gaaggacgac agcaucgaca acaagguccu cacccgaagc     2580

gacaagaacc gcggcaagag cgacaacguu cccucagagg aagucgucaa gaagaugaag     2640

aacuacuggc gccagcuccu caacgccaag cucaucacuc aacgcaaguu cgacaaucuc     2700

accaaggcgg agcgcggugg ccucagcgag cuggacaagg ccggguucau caagcgccag     2760

cucgucgaga cccgccagau caccaagcac gucgcccaga uccucgacag ccgcaugaac     2820

accaaguacg acgagaacga caagcucauc agggaaguca aggucaucac ccucaagagc     2880

aagcucguca gcgacuuccg caaggacuuc caguucuaca aggucaggga gaucaacaac     2940

uaccaccacg cccacgacgc cuaccucaac gcugugguug gcaccgcacu gaucaagaag     3000

uaccccaagc ucgagagcga guucgucuac ggcgacuaca aggucuacga cguccgcaag     3060

augauagcca agagcgagca ggagaucggc aaggccaccg ccaaguacuu cuucuacagc     3120

aacaucauga acuucuucaa gacagagauc acccucgcca auggugagau ccgcaagcgc     3180

ccccucaucg agaccaaugg ugagaccggu gagaucgucu gggacaaggg gcgagacuuc     3240

gccaccgucc gcaagguccu cagcaugccc caggugaaca ucgucaagaa gacagaaguc     3300

cagaccggug gcuucagcaa ggagagcauc cuuccaaagc gcaacagcga caagcucauc     3360

gcccgcaaga aggacuggga ccccaagaag uacgguggcu ucgacagccc caccgucgcc     3420

uacagcgucc ucgucgucgc caaggucgag aaggggaaga gcaagaagcu caagagcguc     3480

aaggagcugc ucggcaucac caucauggag cgaagcagcu ucgagaagaa ccccaucgac     3540

uuccuggaag ccaaggggua caaggaaguc aagaaggacc ucaucaucaa gcuuccaaag     3600

uacagccucu ucgagcugga gaaugggcgc aagcgcaugc ucgccagcgc cggugagcug     3660

cagaagggga acgagcuggc acuucccuca aaguacguca acuuccugua ccucgccagc     3720

cacuacgaga agcucaaggg gagcccagag gacaacgagc agaagcagcu cuucgucgag     3780

cagcacaagc acuaccucga cgagaucauc gagcagauca gcgaguucag caagcgcguc     3840

auccucgccg acgccaaucu cgacaagguc cucagcgccu acaacaagca ccgagacaag     3900

cccaucaggg agcaggccga gaacaucauc caccucuuca cccucaccaa ucucggugcc     3960

ccagcugccu ucaaguacuu cgacaccacc aucgaccgca agcgcuacac cucgacuaag     4020

gaaguccucg acgccacccu cauccaccag agcaucaccg gccucuacga gacccgcauc     4080

gaccucagcc agcucggugg cgacgguggu ggcagcccca agaagaagcg caaggucuag     4140


<210>  10
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: E-pair and E-single enriched Cas9 ORF

<400>  10
auggacaaga aguacagcau cggccucgac aucggcacca acagcgucgg cugggccguc       60

aucaccgacg aguacaaggu ccccagcaag aaguucaagg uccucggcaa caccgaccgc      120

cacagcauca agaagaaccu caucggcgcc cuccucuucg acagcggcga gaccgccgag      180

gccacccgcc ucaagcgcac cgcccgccgc cgcuacaccc gccgcaagaa ccgcaucugc      240

uaccuccagg agaucuucag caacgagaug gccaaggucg acgacagcuu cuuccaccgc      300

cucgaggaga gcuuccucgu cgaggaggac aagaagcacg agcgccaccc caucuucggc      360

aacaucgucg acgaggucgc cuaccacgag aaguacccca ccaucuacca ccuccgcaag      420

aagcucgucg acagcaccga caaggccgac cuccgccuca ucuaccucgc ccucgcccac      480

augaucaagu uccgcggcca cuuccucauc gagggcgacc ucaaccccga caacagcgac      540

gucgacaagc ucuucaucca gcucguccag accuacaacc agcucuucga ggagaacccc      600

aucaacgcca gcggcgucga cgccaaggcc auccucagcg cccgccucag caagagccgc      660

cgccucgaga accucaucgc ccagcucccc ggcgagaaga agaacggccu cuucggcaac      720

cucaucgccc ucagccucgg ccucaccccc aacuucaaga gcaacuucga ccucgccgag      780

gacgccaagc uccagcucag caaggacacc uacgacgacg accucgacaa ccuccucgcc      840

cagaucggcg accaguacgc cgaccucuuc cucgccgcca agaaccucag cgacgccauc      900

cuccucagcg acauccuccg cgucaacacc gagaucacca aggccccccu cagcgccagc      960

augaucaagc gcuacgacga gcaccaccag gaccucaccc uccucaaggc ccucguccgc     1020

cagcagcucc ccgagaagua caaggagauc uucuucgacc agagcaagaa cggcuacgcc     1080

ggcuacaucg acggcggcgc cagccaggag gaguucuaca aguucaucaa gcccauccuc     1140

gagaagaugg acggcaccga ggagcuccuc gucaagcuca accgcgagga ccuccuccgc     1200

aagcagcgca ccuucgacaa cggcagcauc ccccaccaga uccaccucgg cgagcuccac     1260

gccauccucc gccgccagga ggacuucuac cccuuccuca aggacaaccg cgagaagauc     1320

gagaagaucc ucaccuuccg cauccccuac uacgucggcc cccucgcccg cggcaacagc     1380

cgcuucgccu ggaugacccg caagagcgag gagaccauca cccccuggaa cuucgaggag     1440

gucgucgaca agggcgccag cgcccagagc uucaucgagc gcaugaccaa cuucgacaag     1500

aaccucccca acgagaaggu ccuccccaag cacagccucc ucuacgagua cuucaccguc     1560

uacaacgagc ucaccaaggu caaguacguc accgagggca ugcgcaagcc cgccuuccuc     1620

agcggcgagc agaagaaggc caucgucgac cuccucuuca agaccaaccg caaggucacc     1680

gucaagcagc ucaaggagga cuacuucaag aagaucgagu gcuucgacag cgucgagauc     1740

agcggcgucg aggaccgcuu caacgccagc cucggcaccu accacgaccu ccucaagauc     1800

aucaaggaca aggacuuccu cgacaacgag gagaacgagg acauccucga ggacaucguc     1860

cucacccuca cccucuucga ggaccgcgag augaucgagg agcgccucaa gaccuacgcc     1920

caccucuucg acgacaaggu caugaagcag cucaagcgcc gccgcuacac cggcuggggc     1980

cgccucagcc gcaagcucau caacggcauc cgcgacaagc agagcggcaa gaccauccuc     2040

gacuuccuca agagcgacgg cuucgccaac cgcaacuuca ugcagcucau ccacgacgac     2100

agccucaccu ucaaggagga cauccagaag gcccagguca gcggccaggg cgacagccuc     2160

cacgagcaca ucgccaaccu cgccggcagc cccgccauca agaagggcau ccuccagacc     2220

gucaaggucg ucgacgagcu cgucaagguc augggccgcc acaagcccga gaacaucguc     2280

aucgagaugg cccgcgagaa ccagaccacc cagaagggcc agaagaacag ccgcgagcgc     2340

augaagcgca ucgaggaggg caucaaggag cucggcagcc agauccucaa ggagcacccc     2400

gucgagaaca cccagcucca gaacgagaag cucuaccucu acuaccucca gaacggccgc     2460

gacauguacg ucgaccagga gcucgacauc aaccgccuca gcgacuacga cgucgaccac     2520

aucguccccc agagcuuccu caaggacgac agcaucgaca acaagguccu cacccgcagc     2580

gacaagaacc gcggcaagag cgacaacguc cccagcgagg aggucgucaa gaagaugaag     2640

aacuacuggc gccagcuccu caacgccaag cucaucaccc agcgcaaguu cgacaaccuc     2700

accaaggccg agcgcggcgg ccucagcgag cucgacaagg ccggcuucau caagcgccag     2760

cucgucgaga cccgccagau caccaagcac gucgcccaga uccucgacag ccgcaugaac     2820

accaaguacg acgagaacga caagcucauc cgcgagguca aggucaucac ccucaagagc     2880

aagcucguca gcgacuuccg caaggacuuc caguucuaca agguccgcga gaucaacaac     2940

uaccaccacg cccacgacgc cuaccucaac gccgucgucg gcaccgcccu caucaagaag     3000

uaccccaagc ucgagagcga guucgucuac ggcgacuaca aggucuacga cguccgcaag     3060

augaucgcca agagcgagca ggagaucggc aaggccaccg ccaaguacuu cuucuacagc     3120

aacaucauga acuucuucaa gaccgagauc acccucgcca acggcgagau ccgcaagcgc     3180

ccccucaucg agaccaacgg cgagaccggc gagaucgucu gggacaaggg ccgcgacuuc     3240

gccaccgucc gcaagguccu cagcaugccc caggucaaca ucgucaagaa gaccgagguc     3300

cagaccggcg gcuucagcaa ggagagcauc cuccccaagc gcaacagcga caagcucauc     3360

gcccgcaaga aggacuggga ccccaagaag uacggcggcu ucgacagccc caccgucgcc     3420

uacagcgucc ucgucgucgc caaggucgag aagggcaaga gcaagaagcu caagagcguc     3480

aaggagcucc ucggcaucac caucauggag cgcagcagcu ucgagaagaa ccccaucgac     3540

uuccucgagg ccaagggcua caaggagguc aagaaggacc ucaucaucaa gcuccccaag     3600

uacagccucu ucgagcucga gaacggccgc aagcgcaugc ucgccagcgc cggcgagcuc     3660

cagaagggca acgagcucgc ccuccccagc aaguacguca acuuccucua ccucgccagc     3720

cacuacgaga agcucaaggg cagccccgag gacaacgagc agaagcagcu cuucgucgag     3780

cagcacaagc acuaccucga cgagaucauc gagcagauca gcgaguucag caagcgcguc     3840

auccucgccg acgccaaccu cgacaagguc cucagcgccu acaacaagca ccgcgacaag     3900

cccauccgcg agcaggccga gaacaucauc caccucuuca cccucaccaa ccucggcgcc     3960

cccgccgccu ucaaguacuu cgacaccacc aucgaccgca agcgcuacac cagcaccaag     4020

gagguccucg acgccacccu cauccaccag agcaucaccg gccucuacga gacccgcauc     4080

gaccucagcc agcucggcgg cgacggcggc ggcagcccca agaagaagcg caaggucuag     4140


<210>  11
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: E-single depleted Cas9 ORF

<400>  11
auggacaaaa aauacucaau aggccucgac auaggcacca acucagucgg cugggccguc       60

auaaccgacg aguacaaagu ccccucaaaa aaauucaaag uccucggcaa caccgacagg      120

cacucaauaa aaaaaaaccu cauaggcgcc cuccucuucg acucaggcga gaccgccgag      180

gccaccaggc ucaaaaggac cgccaggagg agguacacca ggaggaaaaa caggauaugc      240

uaccuccagg agauauucuc aaacgagaug gccaaagucg acgacucauu cuuccacagg      300

cucgaggagu cauuccucgu cgaggaggac aaaaaacacg agaggcaccc cauauucggc      360

aacauagucg acgaggucgc cuaccacgag aaauacccca ccauauacca ccucaggaaa      420

aaacucgucg acucaaccga caaagccgac cucaggcuca uauaccucgc ccucgcccac      480

augauaaaau ucaggggcca cuuccucaua gagggcgacc ucaaccccga caacucagac      540

gucgacaaac ucuucauaca gcucguccag accuacaacc agcucuucga ggagaacccc      600

auaaacgccu caggcgucga cgccaaagcc auacucucag ccaggcucuc aaaaucaagg      660

aggcucgaga accucauagc ccagcucccc ggcgagaaaa aaaacggccu cuucggcaac      720

cucauagccc ucucacucgg ccucaccccc aacuucaaau caaacuucga ccucgccgag      780

gacgccaaac uccagcucuc aaaagacacc uacgacgacg accucgacaa ccuccucgcc      840

cagauaggcg accaguacgc cgaccucuuc cucgccgcca aaaaccucuc agacgccaua      900

cuccucucag acauacucag ggucaacacc gagauaacca aagccccccu cucagccuca      960

augauaaaaa gguacgacga gcaccaccag gaccucaccc uccucaaagc ccucgucagg     1020

cagcagcucc ccgagaaaua caaagagaua uucuucgacc agucaaaaaa cggcuacgcc     1080

ggcuacauag acggcggcgc cucacaggag gaguucuaca aauucauaaa acccauacuc     1140

gagaaaaugg acggcaccga ggagcuccuc gucaaacuca acagggagga ccuccucagg     1200

aaacagagga ccuucgacaa cggcucaaua ccccaccaga uacaccucgg cgagcuccac     1260

gccauacuca ggaggcagga ggacuucuac cccuuccuca aagacaacag ggagaaaaua     1320

gagaaaauac ucaccuucag gauacccuac uacgucggcc cccucgccag gggcaacuca     1380

agguucgccu ggaugaccag gaaaucagag gagaccauaa cccccuggaa cuucgaggag     1440

gucgucgaca aaggcgccuc agcccaguca uucauagaga ggaugaccaa cuucgacaaa     1500

aaccucccca acgagaaagu ccuccccaaa cacucacucc ucuacgagua cuucaccguc     1560

uacaacgagc ucaccaaagu caaauacguc accgagggca ugaggaaacc cgccuuccuc     1620

ucaggcgagc agaaaaaagc cauagucgac cuccucuuca aaaccaacag gaaagucacc     1680

gucaaacagc ucaaagagga cuacuucaaa aaaauagagu gcuucgacuc agucgagaua     1740

ucaggcgucg aggacagguu caacgccuca cucggcaccu accacgaccu ccucaaaaua     1800

auaaaagaca aagacuuccu cgacaacgag gagaacgagg acauacucga ggacauaguc     1860

cucacccuca cccucuucga ggacagggag augauagagg agaggcucaa aaccuacgcc     1920

caccucuucg acgacaaagu caugaaacag cucaaaagga ggagguacac cggcuggggc     1980

aggcucucaa ggaaacucau aaacggcaua agggacaaac agucaggcaa aaccauacuc     2040

gacuuccuca aaucagacgg cuucgccaac aggaacuuca ugcagcucau acacgacgac     2100

ucacucaccu ucaaagagga cauacagaaa gcccaggucu caggccaggg cgacucacuc     2160

cacgagcaca uagccaaccu cgccggcuca cccgccauaa aaaaaggcau acuccagacc     2220

gucaaagucg ucgacgagcu cgucaaaguc augggcaggc acaaacccga gaacauaguc     2280

auagagaugg ccagggagaa ccagaccacc cagaaaggcc agaaaaacuc aagggagagg     2340

augaaaagga uagaggaggg cauaaaagag cucggcucac agauacucaa agagcacccc     2400

gucgagaaca cccagcucca gaacgagaaa cucuaccucu acuaccucca gaacggcagg     2460

gacauguacg ucgaccagga gcucgacaua aacaggcucu cagacuacga cgucgaccac     2520

auaguccccc agucauuccu caaagacgac ucaauagaca acaaaguccu caccagguca     2580

gacaaaaaca ggggcaaauc agacaacguc cccucagagg aggucgucaa aaaaaugaaa     2640

aacuacugga ggcagcuccu caacgccaaa cucauaaccc agaggaaauu cgacaaccuc     2700

accaaagccg agaggggcgg ccucucagag cucgacaaag ccggcuucau aaaaaggcag     2760

cucgucgaga ccaggcagau aaccaaacac gucgcccaga uacucgacuc aaggaugaac     2820

accaaauacg acgagaacga caaacucaua agggagguca aagucauaac ccucaaauca     2880

aaacucgucu cagacuucag gaaagacuuc caguucuaca aagucaggga gauaaacaac     2940

uaccaccacg cccacgacgc cuaccucaac gccgucgucg gcaccgcccu cauaaaaaaa     3000

uaccccaaac ucgagucaga guucgucuac ggcgacuaca aagucuacga cgucaggaaa     3060

augauagcca aaucagagca ggagauaggc aaagccaccg ccaaauacuu cuucuacuca     3120

aacauaauga acuucuucaa aaccgagaua acccucgcca acggcgagau aaggaaaagg     3180

ccccucauag agaccaacgg cgagaccggc gagauagucu gggacaaagg cagggacuuc     3240

gccaccguca ggaaaguccu cucaaugccc caggucaaca uagucaaaaa aaccgagguc     3300

cagaccggcg gcuucucaaa agagucaaua cuccccaaaa ggaacucaga caaacucaua     3360

gccaggaaaa aagacuggga ccccaaaaaa uacggcggcu ucgacucacc caccgucgcc     3420

uacucagucc ucgucgucgc caaagucgag aaaggcaaau caaaaaaacu caaaucaguc     3480

aaagagcucc ucggcauaac cauaauggag aggucaucau ucgagaaaaa ccccauagac     3540

uuccucgagg ccaaaggcua caaagagguc aaaaaagacc ucauaauaaa acuccccaaa     3600

uacucacucu ucgagcucga gaacggcagg aaaaggaugc ucgccucagc cggcgagcuc     3660

cagaaaggca acgagcucgc ccuccccuca aaauacguca acuuccucua ccucgccuca     3720

cacuacgaga aacucaaagg cucacccgag gacaacgagc agaaacagcu cuucgucgag     3780

cagcacaaac acuaccucga cgagauaaua gagcagauau cagaguucuc aaaaaggguc     3840

auacucgccg acgccaaccu cgacaaaguc cucucagccu acaacaaaca cagggacaaa     3900

cccauaaggg agcaggccga gaacauaaua caccucuuca cccucaccaa ccucggcgcc     3960

cccgccgccu ucaaauacuu cgacaccacc auagacagga aaagguacac cucaaccaaa     4020

gagguccucg acgccacccu cauacaccag ucaauaaccg gccucuacga gaccaggaua     4080

gaccucucac agcucggcgg cgacggcggc ggcucaccca aaaaaaaaag gaaagucuag     4140


<210>  12
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: I-single enriched Cas9 ORF

<400>  12
auggacaaaa aauacagcau cggccuggac aucggcacga acagcguggg cugggccgug       60

aucacggacg aguacaaagu gcccagcaaa aaauucaaag ugcugggcaa cacggaccgg      120

cacagcauca aaaaaaaccu gaucggcgcc cugcuguucg acagcggcga gacggccgag      180

gccacgcggc ugaaacggac ggcccggcgg cgguacacgc ggcggaaaaa ccggaucugc      240

uaccugcagg agaucuucag caacgagaug gccaaagugg acgacagcuu cuuccaccgg      300

cuggaggaga gcuuccuggu ggaggaggac aaaaaacacg agcggcaccc caucuucggc      360

aacaucgugg acgagguggc cuaccacgag aaauacccca cgaucuacca ccugcggaaa      420

aaacuggugg acagcacgga caaagccgac cugcggcuga ucuaccuggc ccuggcccac      480

augaucaaau uccggggcca cuuccugauc gagggcgacc ugaaccccga caacagcgac      540

guggacaaac uguucaucca gcuggugcag acguacaacc agcuguucga ggagaacccc      600

aucaacgcca gcggcgugga cgccaaagcc auccugagcg cccggcugag caaaagccgg      660

cggcuggaga accugaucgc ccagcugccc ggcgagaaaa aaaacggccu guucggcaac      720

cugaucgccc ugagccuggg ccugacgccc aacuucaaaa gcaacuucga ccuggccgag      780

gacgccaaac ugcagcugag caaagacacg uacgacgacg accuggacaa ccugcuggcc      840

cagaucggcg accaguacgc cgaccuguuc cuggccgcca aaaaccugag cgacgccauc      900

cugcugagcg acauccugcg ggugaacacg gagaucacga aagccccccu gagcgccagc      960

augaucaaac gguacgacga gcaccaccag gaccugacgc ugcugaaagc ccuggugcgg     1020

cagcagcugc ccgagaaaua caaagagauc uucuucgacc agagcaaaaa cggcuacgcc     1080

ggcuacaucg acggcggcgc cagccaggag gaguucuaca aauucaucaa acccauccug     1140

gagaaaaugg acggcacgga ggagcugcug gugaaacuga accgggagga ccugcugcgg     1200

aaacagcgga cguucgacaa cggcagcauc ccccaccaga uccaccuggg cgagcugcac     1260

gccauccugc ggcggcagga ggacuucuac cccuuccuga aagacaaccg ggagaaaauc     1320

gagaaaaucc ugacguuccg gauccccuac uacgugggcc cccuggcccg gggcaacagc     1380

cgguucgccu ggaugacgcg gaaaagcgag gagacgauca cgcccuggaa cuucgaggag     1440

gugguggaca aaggcgccag cgcccagagc uucaucgagc ggaugacgaa cuucgacaaa     1500

aaccugccca acgagaaagu gcugcccaaa cacagccugc uguacgagua cuucacggug     1560

uacaacgagc ugacgaaagu gaaauacgug acggagggca ugcggaaacc cgccuuccug     1620

agcggcgagc agaaaaaagc caucguggac cugcuguuca aaacgaaccg gaaagugacg     1680

gugaaacagc ugaaagagga cuacuucaaa aaaaucgagu gcuucgacag cguggagauc     1740

agcggcgugg aggaccgguu caacgccagc cugggcacgu accacgaccu gcugaaaauc     1800

aucaaagaca aagacuuccu ggacaacgag gagaacgagg acauccugga ggacaucgug     1860

cugacgcuga cgcuguucga ggaccgggag augaucgagg agcggcugaa aacguacgcc     1920

caccuguucg acgacaaagu gaugaaacag cugaaacggc ggcgguacac gggcuggggc     1980

cggcugagcc ggaaacugau caacggcauc cgggacaaac agagcggcaa aacgauccug     2040

gacuuccuga aaagcgacgg cuucgccaac cggaacuuca ugcagcugau ccacgacgac     2100

agccugacgu ucaaagagga cauccagaaa gcccagguga gcggccaggg cgacagccug     2160

cacgagcaca ucgccaaccu ggccggcagc cccgccauca aaaaaggcau ccugcagacg     2220

gugaaagugg uggacgagcu ggugaaagug augggccggc acaaacccga gaacaucgug     2280

aucgagaugg cccgggagaa ccagacgacg cagaaaggcc agaaaaacag ccgggagcgg     2340

augaaacgga ucgaggaggg caucaaagag cugggcagcc agauccugaa agagcacccc     2400

guggagaaca cgcagcugca gaacgagaaa cuguaccugu acuaccugca gaacggccgg     2460

gacauguacg uggaccagga gcuggacauc aaccggcuga gcgacuacga cguggaccac     2520

aucgugcccc agagcuuccu gaaagacgac agcaucgaca acaaagugcu gacgcggagc     2580

gacaaaaacc ggggcaaaag cgacaacgug cccagcgagg agguggugaa aaaaaugaaa     2640

aacuacuggc ggcagcugcu gaacgccaaa cugaucacgc agcggaaauu cgacaaccug     2700

acgaaagccg agcggggcgg ccugagcgag cuggacaaag ccggcuucau caaacggcag     2760

cugguggaga cgcggcagau cacgaaacac guggcccaga uccuggacag ccggaugaac     2820

acgaaauacg acgagaacga caaacugauc cgggagguga aagugaucac gcugaaaagc     2880

aaacugguga gcgacuuccg gaaagacuuc caguucuaca aagugcggga gaucaacaac     2940

uaccaccacg cccacgacgc cuaccugaac gccguggugg gcacggcccu gaucaaaaaa     3000

uaccccaaac uggagagcga guucguguac ggcgacuaca aaguguacga cgugcggaaa     3060

augaucgcca aaagcgagca ggagaucggc aaagccacgg ccaaauacuu cuucuacagc     3120

aacaucauga acuucuucaa aacggagauc acgcuggcca acggcgagau ccggaaacgg     3180

ccccugaucg agacgaacgg cgagacgggc gagaucgugu gggacaaagg ccgggacuuc     3240

gccacggugc ggaaagugcu gagcaugccc caggugaaca ucgugaaaaa aacggaggug     3300

cagacgggcg gcuucagcaa agagagcauc cugcccaaac ggaacagcga caaacugauc     3360

gcccggaaaa aagacuggga ccccaaaaaa uacggcggcu ucgacagccc cacgguggcc     3420

uacagcgugc uggugguggc caaaguggag aaaggcaaaa gcaaaaaacu gaaaagcgug     3480

aaagagcugc ugggcaucac gaucauggag cggagcagcu ucgagaaaaa ccccaucgac     3540

uuccuggagg ccaaaggcua caaagaggug aaaaaagacc ugaucaucaa acugcccaaa     3600

uacagccugu ucgagcugga gaacggccgg aaacggaugc uggccagcgc cggcgagcug     3660

cagaaaggca acgagcuggc ccugcccagc aaauacguga acuuccugua ccuggccagc     3720

cacuacgaga aacugaaagg cagccccgag gacaacgagc agaaacagcu guucguggag     3780

cagcacaaac acuaccugga cgagaucauc gagcagauca gcgaguucag caaacgggug     3840

auccuggccg acgccaaccu ggacaaagug cugagcgccu acaacaaaca ccgggacaaa     3900

cccauccggg agcaggccga gaacaucauc caccuguuca cgcugacgaa ccugggcgcc     3960

cccgccgccu ucaaauacuu cgacacgacg aucgaccgga aacgguacac gagcacgaaa     4020

gaggugcugg acgccacgcu gauccaccag agcaucacgg gccuguacga gacgcggauc     4080

gaccugagcc agcugggcgg cgacggcggc ggcagcccca aaaaaaaacg gaaaguguag     4140


<210>  13
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: E-pair depleted Cas9 ORF

<400>  13
auggauaaaa aauauucaau aggauuagau auaggaacaa auucaguagg augggcagua       60

auaacagaug aauauaaagu accaucaaaa aaauuuaaag uauuaggaaa uacagauaga      120

cauucaauaa aaaaaaauuu aauaggagca uuauuauuug auucaggaga aacagcagaa      180

gcaacaagau uaaaaagaac agcaagaaga agauauacaa gaagaaaaaa uagaauaugu      240

uauuuacaag aaauauuuuc aaaugaaaug gcaaaaguag augauucauu uuuucauaga      300

uuagaagaau cauuuuuagu agaagaagau aaaaaacaug aaagacaucc aauauuugga      360

aauauaguag augaaguagc auaucaugaa aaauauccaa caauauauca uuuaagaaaa      420

aaauuaguag auucaacaga uaaagcagau uuaagauuaa uauauuuagc auuagcacau      480

augauaaaau uuagaggaca uuuuuuaaua gaaggagauu uaaauccaga uaauucagau      540

guagauaaau uauuuauaca auuaguacaa acauauaauc aauuauuuga agaaaaucca      600

auaaaugcau caggaguaga ugcaaaagca auauuaucag caagauuauc aaaaucaaga      660

agauuagaaa auuuaauagc acaauuacca ggagaaaaaa aaaauggauu auuuggaaau      720

uuaauagcau uaucauuagg auuaacacca aauuuuaaau caaauuuuga uuuagcagaa      780

gaugcaaaau uacaauuauc aaaagauaca uaugaugaug auuuagauaa uuuauuagca      840

caaauaggag aucaauaugc agauuuauuu uuagcagcaa aaaauuuauc agaugcaaua      900

uuauuaucag auauauuaag aguaaauaca gaaauaacaa aagcaccauu aucagcauca      960

augauaaaaa gauaugauga acaucaucag gacuuaacau uauuaaaagc auuaguaaga     1020

caacaauuac cagaaaaaua uaaagaaaua uuuuuugauc aaucaaaaaa uggauaugca     1080

ggauauauag auggaggagc aucacaagaa gaauuuuaua aauuuauaaa accaauauua     1140

gaaaaaaugg auggaacaga agaauuauua guaaaauuaa auagagaaga uuuauuaaga     1200

aaacaaagaa cauuugauaa uggaucaaua ccacaucaaa uacauuuagg agaauuacau     1260

gcaauauuaa gaagacaaga agauuuuuau ccauuuuuaa aagauaauag agaaaaaaua     1320

gaaaaaauau uaacauuuag aauaccauau uauguaggac cauuagcaag aggaaauuca     1380

agauuugcau ggaugacaag aaaaucagaa gaaacaauaa caccauggaa uuuugaagaa     1440

guaguagaua aaggagcauc agcacaauca uuuauagaaa gaaugacaaa uuuugauaaa     1500

aauuuaccaa augaaaaagu auuaccaaaa cauucauuau uauaugaaua uuuuacagua     1560

uauaaugaau uaacaaaagu aaaauaugua acagaaggaa ugagaaaacc agcauuuuua     1620

ucaggagaac aaaaaaaagc aauaguagau uuauuauuua aaacaaauag aaaaguaaca     1680

guaaaacaau uaaaagaaga uuauuuuaaa aaaauagaau guuuugauuc aguagaaaua     1740

ucaggaguag aagauagauu uaaugcauca uuaggaacau aucaugauuu auuaaaaaua     1800

auaaaagaua aagauuuuuu agauaaugaa gaaaaugaag auauauuaga agauauagua     1860

uuaacauuaa cauuauuuga agauagagaa augauagaag aaagauuaaa aacauaugca     1920

cauuuauuug augauaaagu aaugaaacaa uuaaaaagaa gaagauauac aggaugggga     1980

agauuaucaa gaaaauuaau aaauggaaua agagauaaac aaucaggaaa aacaauauua     2040

gauuuuuuaa aaucagaugg auuugcaaau agaaauuuua ugcaauuaau acaugaugau     2100

ucauuaacau uuaaagaaga uauacaaaaa gcacaaguau caggacaagg agauucauua     2160

caugaacaua uagcaaauuu agcaggauca ccagcaauaa aaaaaggaau auuacaaaca     2220

guaaaaguag uagaugaauu aguaaaagua augggaagac auaaaccaga aaauauagua     2280

auagaaaugg caagagaaaa ucaaacaaca caaaaaggac aaaaaaauuc aagagaaaga     2340

augaaaagaa uagaagaagg aauaaaagaa uuaggaucac aaauauuaaa agaacaucca     2400

guagaaaaua cacaauuaca aaaugaaaaa uuauauuuau auuauuuaca aaauggaaga     2460

gauauguaug uagaucaaga auuagauaua aauagauuau cagauuauga uguagaucau     2520

auaguaccac aaucauuuuu aaaagaugau ucaauagaua auaaaguauu aacaagauca     2580

gauaaaaaua gaggaaaauc agauaaugua ccaucagaag aaguaguaaa aaaaaugaaa     2640

aauuauugga gacaauuauu aaaugcaaaa uuaauaacac aaagaaaauu ugauaauuua     2700

acaaaagcag aaagaggagg auuaucagaa uuagauaaag caggauuuau aaaaagacaa     2760

uuaguagaaa caagacaaau aacaaaacau guagcacaaa uauuagauuc aagaaugaau     2820

acaaaauaug augaaaauga uaaauuaaua agagaaguaa aaguaauaac auuaaaauca     2880

aaauuaguau cagauuuuag aaaagauuuu caauuuuaua aaguaagaga aauaaauaau     2940

uaucaucaug cacaugaugc auauuuaaau gcaguaguag gaacagcauu aauaaaaaaa     3000

uauccaaaau uagaaucaga auuuguauau ggagauuaua aaguauauga uguaagaaaa     3060

augauagcaa aaucagaaca agaaauagga aaagcaacag caaaauauuu uuuuuauuca     3120

aauauaauga auuuuuuuaa aacagaaaua acauuagcaa auggagaaau aagaaaaaga     3180

ccauuaauag aaacaaaugg agaaacagga gaaauaguau gggauaaagg aagagauuuu     3240

gcaacaguaa gaaaaguauu aucaaugcca caaguaaaua uaguaaaaaa aacagaagua     3300

caaacaggag gauuuucaaa agaaucaaua uuaccaaaaa gaaauucaga uaaauuaaua     3360

gcaagaaaaa aagauuggga uccaaaaaaa uauggaggau uugauucacc aacaguagca     3420

uauucaguau uaguaguagc aaaaguagaa aaaggaaaau caaaaaaauu aaaaucagua     3480

aaagaauuau uaggaauaac aauaauggaa agaucaucau uugaaaaaaa uccaauagau     3540

uuuuuagaag caaaaggaua uaaagaagua aaaaaagauu uaauaauaaa auuaccaaaa     3600

uauucauuau uugaauuaga aaauggaaga aaaagaaugu uagcaucagc aggagaauua     3660

caaaaaggaa augaauuagc auuaccauca aaauauguaa auuuuuuaua uuuagcauca     3720

cauuaugaaa aauuaaaagg aucaccagaa gauaaugaac aaaaacaauu auuuguagaa     3780

caacauaaac auuauuuaga ugaaauaaua gaacaaauau cagaauuuuc aaaaagagua     3840

auauuagcag augcaaauuu agauaaagua uuaucagcau auaauaaaca uagagauaaa     3900

ccaauaagag aacaagcaga aaauauaaua cauuuauuua cauuaacaaa uuuaggagca     3960

ccagcagcau uuaaauauuu ugauacaaca auagauagaa aaagauauac aucaacaaaa     4020

gaaguauuag augcaacauu aauacaucaa ucaauaacag gauuauauga aacaagaaua     4080

gauuuaucac aauuaggagg agauggagga ggaucaccaa aaaaaaaaag aaaaguauag     4140


<210>  14
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: I-pair enriched Cas9 ORF

<400>  14
auggauaaaa aguacagcau cggauuagau auaggaacaa auucaguugg cugggcugug       60

auaacagaug aauauaaagu ucccucaaaa aaauuuaaag uauuaggaaa uacagauaga      120

cauagcauca aaaaaaaucu cauaggugca cuguuauuug auucagguga gacagcagaa      180

gccacaagau uaaaaagaac agcccgcaga agauauacaa gaagaaaaaa uagaauaugu      240

uauuuacagg agauauuuuc aaaugaaaug gcaaaaguag augauucauu uuuucauaga      300

uuagaagaau cauuccuggu agaagaagau aaaaaacaug aaagacaucc aauauuugga      360

aauauaguag augaagucgc auaucaugaa aaguacccca ccauauauca ucugcggaaa      420

aaauuaguag auucgacuga uaaagcagau cugcgguuaa uauauuuagc acuggcacau      480

augauaaaau uuagaggaca uuuccugaua gaaggagauu uaaauccuga caauucagau      540

guagauaaau uauuuauaca auuaguacaa accuacaauc aauuauuuga agaaaaucca      600

auaaaugcau caggaguaga ugcaaaagca auacucagcg cccgccucag caaaucaaga      660

agauuagaaa aucucauagc acaacuucca ggugagaaaa aaaauggguu auuuggaaau      720

cucauagcac ucagcuuagg auuaacuccc aauuuuaaau caaauuuuga uuuagcagaa      780

gaugcaaaau uacaacucag caaagauacc uacgaugaug auuuagauaa ucucuuagca      840

caaauaggag aucaauaugc agauuuauuc cuggcugcca aaaaucucag cgaugcaaua      900

uuacucagcg auauacugcg gguaaauaca gagauaacaa aagcaccacu cagcgcauca      960

augauaaaaa gauaugauga acaucaucaa gauuuaacau uauuaaaagc acugguaaga     1020

caacaacuuc cagagaagua caaagaaaua uuuuuugauc agagcaaaaa uggguaugcc     1080

ggguauauag augguggugc cucacaggag gaauuuuaua aauuuauaaa accaauauua     1140

gaaaaaaugg auggaacaga ggagcuguua guaaaauuaa auagggagga uuuacugcgg     1200

aaacaaagaa cauuugauaa ugggagcauc ccccaucaaa uacauuuagg ugagcugcau     1260

gcaauacugc ggagacagga ggauuuuuau ccauuccuga aagauaauag ggagaaaaua     1320

gaaaaaauau uaacauuuag aauccccuau uauguuggcc cauuagcccg cggaaauuca     1380

agauuugcau ggaugacaag aaaaucagaa gaaacaauaa cucccuggaa uuuugaagaa     1440

gucguagaua agggugccuc agcacagagc uuuauagaaa gaaugacaaa uuuugauaaa     1500

aaucuuccaa augaaaaagu acuuccaaaa cauucauuau uauaugaaua uuuuacagua     1560

uauaaugagc ugacaaaagu aaaguacgua acagagggaa ugagaaaacc agcauuccuc     1620

agcggugagc aaaaaaaagc aauaguagau uuauuauuua aaacaaauag aaaaguaaca     1680

guaaaacaau uaaaagaaga uuauuuuaaa aaaauagaau guuuugauuc aguagaaaua     1740

ucaggaguag aagauagauu uaaugcauca uuaggaaccu accaugauuu auuaaaaaua     1800

auaaaagaua aagauuuccu ggauaaugaa gaaaaugaag auauauuaga agauauagua     1860

uuaacauuaa cauuauuuga agauagggag augauagaag aaagauuaaa aaccuacgca     1920

cauuuauuug augauaaagu aaugaaacaa uuaaaaagaa gaagauauac aggaugggga     1980

agacucagca gaaaauuaau aaaugggaua cgagacaaac agagcggaaa aacaauauua     2040

gauuuccuga aaucagaugg auuugcaaau agaaauuuua ugcaauuaau acaugaugau     2100

ucauuaacau uuaaagaaga uauacaaaaa gcacagguca gcggacaggg cgauucauua     2160

caugaacaua uagcaaaucu cgccggguca ccagcaauaa aaaaggggau auuacaaaca     2220

guaaaaguag uagaugagcu gguaaaagua augggaagac auaaaccaga gaauauagua     2280

auagaaaugg ccagggagaa ucaaacaacu caaaaggggc aaaaaaauuc aagggagaga     2340

augaaaagaa uagaagaagg aauaaaagag cugggaucac aaauauuaaa agaacaucca     2400

guagaaaaua cucaauuaca aaaugaaaaa uuauauuuau auuauuuaca aaaugggcga     2460

gacauguaug uagaucagga gcuggauaua aauagacuca gcgauuauga uguagaucau     2520

auaguucccc agagcuuccu gaaagaugau agcaucgaua auaaaguauu aacaagauca     2580

gauaaaaaua gaggaaaauc agauaauguu cccucagaag aagucguaaa aaaaaugaaa     2640

aauuauugga gacaauuauu aaaugcaaaa uuaauaacuc aaagaaaauu ugauaaucuc     2700

acaaaagcag aaagaggugg ccucagcgag cuggauaaag ccggguuuau aaaaagacaa     2760

uuaguagaaa caagacaaau aacaaaacau guagcacaaa uauuagauuc aagaaugaau     2820

acaaaguacg augaaaauga uaaauuaaua agggaaguca aaguaauaac auuaaaauca     2880

aaauuaguca gcgauuuuag aaaagauuuu caauuuuaua aaguaaggga gauaaauaau     2940

uaucaucaug cacaugaugc auauuuaaau gcugugguug gcacagcacu gauaaaaaag     3000

uacccaaaau uagaaucaga auuuguauau ggagauuaua aaguauauga uguaagaaaa     3060

augauagcaa aaucagaaca ggagauagga aaagcaacag caaaguacuu uuuuuauuca     3120

aauauaauga auuuuuuuaa aacagagaua acauuagcaa auggugagau aagaaaaaga     3180

ccauuaauag aaacaaaugg ugagacaggu gagauaguau gggauaaggg gcgagacuuu     3240

gcaacaguaa gaaaaguacu cagcaugcca caggugaaua uaguaaaaaa aacagaaguc     3300

caaacaggug gcuuuucaaa agaaagcauc cuuccaaaaa gaaauucaga uaaauuaaua     3360

gcccgcaaaa aagauuggga uccaaaaaag uacgguggcu uugauucacc caccguagca     3420

uauucaguau uaguaguagc aaaaguagaa aaggggaaau caaaaaaauu aaaaucagua     3480

aaagagcugu uaggaauaac aauaauggaa agaucaucau uugaaaaaaa uccaauagau     3540

uuccuggaag ccaaggggua uaaagaaguc aaaaaagauu uaauaauaaa acuuccaaag     3600

uacucauuau uugagcugga aaaugggaga aaaagaaugu uagcaucagc cggugagcug     3660

caaaagggga augagcuggc acuucccuca aaguacguaa auuuccugua uuuagcauca     3720

cauuaugaaa aauuaaaggg gucaccagag gauaaugaac aaaaacaauu auuuguagaa     3780

caacauaaac auuauuuaga ugaaauaaua gaacaaauau cagaauuuuc aaaaagagua     3840

auauuagcag augcaaaucu cgauaaagua cucagcgcau auaauaaaca ucgagacaaa     3900

ccaauaaggg agcaggccga aaauauaaua cauuuauuua cauuaacaaa ucucggugcc     3960

ccagcugccu uuaaguacuu ugauacaaca auagauagaa aaagauauac aucgacuaaa     4020

gaagucuuag augcaacauu aauacaucag agcaucacag gauuauauga aacaagaaua     4080

gaucucagcc aauuaggugg cgaugguggu ggcucaccaa aaaaaaaaag aaaaguauag     4140


<210>  15
<211>  4425
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 mRNA transcript comprising SEQ 5

<400>  15
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguaca       60

gcaucggccu ggacaucggc acgaacagcg ugggcugggc cgugaucacg gacgaguaca      120

aggugcccag caagaaguuc aaggugcugg gcaacacgga ccggcacagc aucaagaaga      180

accugaucgg cgcccugcug uucgacagcg gcgagacggc cgaggccacg cggcugaagc      240

ggacggcccg gcggcgguac acgcggcgga agaaccggau cugcuaccug caggagaucu      300

ucagcaacga gauggccaag guggacgaca gcuucuucca ccggcuggag gagagcuucc      360

ugguggagga ggacaagaag cacgagcggc accccaucuu cggcaacauc guggacgagg      420

uggccuacca cgagaaguac cccacgaucu accaccugcg gaagaagcug guggacagca      480

cggacaaggc cgaccugcgg cugaucuacc uggcccuggc ccacaugauc aaguuccggg      540

gccacuuccu gaucgagggc gaccugaacc ccgacaacag cgacguggac aagcuguuca      600

uccagcuggu gcagacguac aaccagcugu ucgaggagaa ccccaucaac gccagcggcg      660

uggacgccaa ggccauccug agcgcccggc ugagcaagag ccggcggcug gagaaccuga      720

ucgcccagcu gcccggcgag aagaagaacg gccuguucgg caaccugauc gcccugagcc      780

ugggccugac gcccaacuuc aagagcaacu ucgaccuggc cgaggacgcc aagcugcagc      840

ugagcaagga cacguacgac gacgaccugg acaaccugcu ggcccagauc ggcgaccagu      900

acgccgaccu guuccuggcc gccaagaacc ugagcgacgc cauccugcug agcgacaucc      960

ugcgggugaa cacggagauc acgaaggccc cccugagcgc cagcaugauc aagcgguacg     1020

acgagcacca ccaggaccug acgcugcuga aggcccuggu gcggcagcag cugcccgaga     1080

aguacaagga gaucuucuuc gaccagagca agaacggcua cgccggcuac aucgacggcg     1140

gcgccagcca ggaggaguuc uacaaguuca ucaagcccau ccuggagaag auggacggca     1200

cggaggagcu gcuggugaag cugaaccggg aggaccugcu gcggaagcag cggacguucg     1260

acaacggcag caucccccac cagauccacc ugggcgagcu gcacgccauc cugcggcggc     1320

aggaggacuu cuaccccuuc cugaaggaca accgggagaa gaucgagaag auccugacgu     1380

uccggauccc cuacuacgug ggcccccugg cccggggcaa cagccgguuc gccuggauga     1440

cgcggaagag cgaggagacg aucacgcccu ggaacuucga ggagguggug gacaagggcg     1500

ccagcgccca gagcuucauc gagcggauga cgaacuucga caagaaccug cccaacgaga     1560

aggugcugcc caagcacagc cugcuguacg aguacuucac gguguacaac gagcugacga     1620

aggugaagua cgugacggag ggcaugcgga agcccgccuu ccugagcggc gagcagaaga     1680

aggccaucgu ggaccugcug uucaagacga accggaaggu gacggugaag cagcugaagg     1740

aggacuacuu caagaagauc gagugcuucg acagcgugga gaucagcggc guggaggacc     1800

gguucaacgc cagccugggc acguaccacg accugcugaa gaucaucaag gacaaggacu     1860

uccuggacaa cgaggagaac gaggacaucc uggaggacau cgugcugacg cugacgcugu     1920

ucgaggaccg ggagaugauc gaggagcggc ugaagacgua cgcccaccug uucgacgaca     1980

aggugaugaa gcagcugaag cggcggcggu acacgggcug gggccggcug agccggaagc     2040

ugaucaacgg cauccgggac aagcagagcg gcaagacgau ccuggacuuc cugaagagcg     2100

acggcuucgc caaccggaac uucaugcagc ugauccacga cgacagccug acguucaagg     2160

aggacaucca gaaggcccag gugagcggcc agggcgacag ccugcacgag cacaucgcca     2220

accuggccgg cagccccgcc aucaagaagg gcauccugca gacggugaag gugguggacg     2280

agcuggugaa ggugaugggc cggcacaagc ccgagaacau cgugaucgag auggcccggg     2340

agaaccagac gacgcagaag ggccagaaga acagccggga gcggaugaag cggaucgagg     2400

agggcaucaa ggagcugggc agccagaucc ugaaggagca ccccguggag aacacgcagc     2460

ugcagaacga gaagcuguac cuguacuacc ugcagaacgg ccgggacaug uacguggacc     2520

aggagcugga caucaaccgg cugagcgacu acgacgugga ccacaucgug ccccagagcu     2580

uccugaagga cgacagcauc gacaacaagg ugcugacgcg gagcgacaag aaccggggca     2640

agagcgacaa cgugcccagc gaggaggugg ugaagaagau gaagaacuac uggcggcagc     2700

ugcugaacgc caagcugauc acgcagcgga aguucgacaa ccugacgaag gccgagcggg     2760

gcggccugag cgagcuggac aaggccggcu ucaucaagcg gcagcuggug gagacgcggc     2820

agaucacgaa gcacguggcc cagauccugg acagccggau gaacacgaag uacgacgaga     2880

acgacaagcu gauccgggag gugaagguga ucacgcugaa gagcaagcug gugagcgacu     2940

uccggaagga cuuccaguuc uacaaggugc gggagaucaa caacuaccac cacgcccacg     3000

acgccuaccu gaacgccgug gugggcacgg cccugaucaa gaaguacccc aagcuggaga     3060

gcgaguucgu guacggcgac uacaaggugu acgacgugcg gaagaugauc gccaagagcg     3120

agcaggagau cggcaaggcc acggccaagu acuucuucua cagcaacauc augaacuucu     3180

ucaagacgga gaucacgcug gccaacggcg agauccggaa gcggccccug aucgagacga     3240

acggcgagac gggcgagauc gugugggaca agggccggga cuucgccacg gugcggaagg     3300

ugcugagcau gccccaggug aacaucguga agaagacgga ggugcagacg ggcggcuuca     3360

gcaaggagag cauccugccc aagcggaaca gcgacaagcu gaucgcccgg aagaaggacu     3420

gggaccccaa gaaguacggc ggcuucgaca gccccacggu ggccuacagc gugcuggugg     3480

uggccaaggu ggagaagggc aagagcaaga agcugaagag cgugaaggag cugcugggca     3540

ucacgaucau ggagcggagc agcuucgaga agaaccccau cgacuuccug gaggccaagg     3600

gcuacaagga ggugaagaag gaccugauca ucaagcugcc caaguacagc cuguucgagc     3660

uggagaacgg ccggaagcgg augcuggcca gcgccggcga gcugcagaag ggcaacgagc     3720

uggcccugcc cagcaaguac gugaacuucc uguaccuggc cagccacuac gagaagcuga     3780

agggcagccc cgaggacaac gagcagaagc agcuguucgu ggagcagcac aagcacuacc     3840

uggacgagau caucgagcag aucagcgagu ucagcaagcg ggugauccug gccgacgcca     3900

accuggacaa ggugcugagc gccuacaaca agcaccggga caagcccauc cgggagcagg     3960

ccgagaacau cauccaccug uucacgcuga cgaaccuggg cgcccccgcc gccuucaagu     4020

acuucgacac gacgaucgac cggaagcggu acacgagcac gaaggaggug cuggacgcca     4080

cgcugaucca ccagagcauc acgggccugu acgagacgcg gaucgaccug agccagcugg     4140

gcggcgacgg cggcggcagc cccaagaaga agcggaaggu guagcuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucuc     4320

ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ucuag                     4425


<210>  16
<211>  4425
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 mRNA transcript comprising SEQ 7

<400>  16
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguaca       60

gcaucggccu cgacaucggc accaacagcg uuggcugggc ugugaucacc gacgaguaca      120

agguucccuc aaagaaguuc aagguccucg gcaacaccga ccgccacagc aucaagaaga      180

aucucaucgg ugcacugcuc uucgacagcg gugagaccgc ggaagccacc cgccucaagc      240

ggaccgcccg ccgccgcuac acccgccgca agaaccgcau cugcuaccuc caggagaucu      300

ucagcaacga gauggccaag gucgacgaca gcuucuucca ccgccucgag gagagcuucc      360

uggucgagga ggacaagaag cacgagcgcc accccaucuu cggcaacauc gucgacgaag      420

ucgccuacca cgagaaguac cccaccaucu accaccugcg gaagaagcuc gucgacucga      480

cugacaaggc cgaccugcgg cucaucuacc ucgcacuggc ccacaugaua aaguuccgcg      540

gccacuuccu gaucgagggc gaccucaacc cugacaacag cgacgucgac aagcucuuca      600

uccagcucgu ccagaccuac aaccagcucu ucgaggagaa ccccaucaac gccagcggcg      660

ucgacgccaa ggccauccuc agcgcccgcc ucagcaagag ccgccgccuc gagaaucuca      720

ucgcccagcu uccaggugag aagaagaaug ggcucuucgg caaucucauc gcacucagcc      780

ucggccucac ucccaacuuc aagagcaacu ucgaccucgc ggaggacgcc aagcuccagc      840

ucagcaagga caccuacgac gacgaccucg acaaucuccu cgcccagauc ggcgaccagu      900

acgccgaccu cuuccuggcu gccaagaauc ucagcgacgc cauccuccuc agcgacaucc      960

ugcgggucaa cacagagauc accaaggccc cccucagcgc cagcaugaua aagcgcuacg     1020

acgagcacca ccaggaccuc acccuccuca aggcacuggu ccgccagcag cuuccagaga     1080

aguacaagga gaucuucuuc gaccagagca agaaugggua cgccggguac aucgacggug     1140

gugccagcca ggaggaguuc uacaaguuca ucaagcccau ccucgagaag auggacggca     1200

cagaggagcu gcucgucaag cucaacaggg aggaccuccu gcggaagcag cggaccuucg     1260

acaaugggag caucccccac cagauccacc ucggugagcu gcacgccauc cugcggcgcc     1320

aggaggacuu cuaccccuuc cugaaggaca acagggagaa gaucgagaag auccucaccu     1380

uccgcauccc cuacuacguu ggcccccucg cccgcggcaa cagccgcuuc gccuggauga     1440

cccgcaagag cgaggagacc aucacucccu ggaacuucga ggaagucguc gacaagggug     1500

ccagcgccca gagcuucauc gagcgcauga ccaacuucga caagaaucuu ccaaacgaga     1560

agguccuucc aaagcacagc cuccucuacg aguacuucac cgucuacaac gagcugacca     1620

aggucaagua cgucacagag ggcaugcgca agccagccuu ccucagcggu gagcagaaga     1680

aggccaucgu cgaccuccuc uucaagacca accgcaaggu caccgucaag cagcucaagg     1740

aggacuacuu caagaagauc gagugcuucg acagcgucga gaucagcggc gucgaggacc     1800

gcuucaacgc cagccucggc accuaccacg accuccucaa gaucaucaag gacaaggacu     1860

uccuggacaa cgaggagaac gaggacaucc ucgaggacau cguccucacc cucacccucu     1920

ucgaggacag ggagaugaua gaggagcgcc ucaagaccua cgcccaccuc uucgacgaca     1980

aggucaugaa gcagcucaag cgccgccgcu acaccggcug gggccgccuc agccgcaagc     2040

ucaucaaugg gauccgagac aagcagagcg gcaagaccau ccucgacuuc cugaagagcg     2100

acggcuucgc caaccgcaac uucaugcagc ucauccacga cgacagccuc accuucaagg     2160

aggacaucca gaaggcccag gucagcggcc agggcgacag ccuccacgag cacaucgcca     2220

aucucgccgg gagcccagcc aucaagaagg ggauccucca gaccgucaag gucgucgacg     2280

agcuggucaa ggucaugggc cgccacaagc cagagaacau cgucaucgag auggccaggg     2340

agaaccagac cacucaaaag gggcagaaga acagcaggga gcgcaugaag cgcaucgagg     2400

agggcaucaa ggagcugggc agccagaucc ucaaggagca ccccgucgag aacacucaac     2460

uccagaacga gaagcucuac cucuacuacc uccagaaugg gcgagacaug uacgucgacc     2520

aggagcugga caucaaccgc cucagcgacu acgacgucga ccacaucguu ccccagagcu     2580

uccugaagga cgacagcauc gacaacaagg uccucacccg aagcgacaag aaccgcggca     2640

agagcgacaa cguucccuca gaggaagucg ucaagaagau gaagaacuac uggcgccagc     2700

uccucaacgc caagcucauc acucaacgca aguucgacaa ucucaccaag gcggagcgcg     2760

guggccucag cgagcuggac aaggccgggu ucaucaagcg ccagcucguc gagacccgcc     2820

agaucaccaa gcacgucgcc cagauccucg acagccgcau gaacaccaag uacgacgaga     2880

acgacaagcu caucagggaa gucaagguca ucacccucaa gagcaagcuc gucagcgacu     2940

uccgcaagga cuuccaguuc uacaagguca gggagaucaa caacuaccac cacgcccacg     3000

acgccuaccu caacgcugug guuggcaccg cacugaucaa gaaguacccc aagcucgaga     3060

gcgaguucgu cuacggcgac uacaaggucu acgacguccg caagaugaua gccaagagcg     3120

agcaggagau cggcaaggcc accgccaagu acuucuucua cagcaacauc augaacuucu     3180

ucaagacaga gaucacccuc gccaauggug agauccgcaa gcgcccccuc aucgagacca     3240

auggugagac cggugagauc gucugggaca aggggcgaga cuucgccacc guccgcaagg     3300

uccucagcau gccccaggug aacaucguca agaagacaga aguccagacc gguggcuuca     3360

gcaaggagag cauccuucca aagcgcaaca gcgacaagcu caucgcccgc aagaaggacu     3420

gggaccccaa gaaguacggu ggcuucgaca gccccaccgu cgccuacagc guccucgucg     3480

ucgccaaggu cgagaagggg aagagcaaga agcucaagag cgucaaggag cugcucggca     3540

ucaccaucau ggagcgaagc agcuucgaga agaaccccau cgacuuccug gaagccaagg     3600

gguacaagga agucaagaag gaccucauca ucaagcuucc aaaguacagc cucuucgagc     3660

uggagaaugg gcgcaagcgc augcucgcca gcgccgguga gcugcagaag gggaacgagc     3720

uggcacuucc cucaaaguac gucaacuucc uguaccucgc cagccacuac gagaagcuca     3780

aggggagccc agaggacaac gagcagaagc agcucuucgu cgagcagcac aagcacuacc     3840

ucgacgagau caucgagcag aucagcgagu ucagcaagcg cgucauccuc gccgacgcca     3900

aucucgacaa gguccucagc gccuacaaca agcaccgaga caagcccauc agggagcagg     3960

ccgagaacau cauccaccuc uucacccuca ccaaucucgg ugccccagcu gccuucaagu     4020

acuucgacac caccaucgac cgcaagcgcu acaccucgac uaaggaaguc cucgacgcca     4080

cccucaucca ccagagcauc accggccucu acgagacccg caucgaccuc agccagcucg     4140

guggcgacgg ugguggcagc cccaagaaga agcgcaaggu cuagcuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucuc     4320

ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ucuag                     4425


<210>  17
<211>  4425
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 mRNA transcript comprising SEQ 9

<400>  17
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguaca       60

gcaucggccu cgacaucggc accaacagcg uuggcugggc ugugaucacc gacgaguaca      120

agguucccuc aaagaaguuc aagguccucg gcaacaccga ccgccacagc aucaagaaga      180

aucucaucgg ugcacugcuc uucgacagcg gugagaccgc ggaagccacc cgccucaagc      240

gcaccgcccg ccgccgcuac acccgccgca agaaccgcau cugcuaccuc caggagaucu      300

ucagcaacga gauggccaag gucgacgaca gcuucuucca ccgccucgag gagagcuucc      360

uggucgagga ggacaagaag cacgagcgcc accccaucuu cggcaacauc gucgacgaag      420

ucgccuacca cgagaaguac cccaccaucu accaccugcg gaagaagcuc gucgacucga      480

cugacaaggc cgaccugcgg cucaucuacc ucgcacuggc ccacaugaua aaguuccgcg      540

gccacuuccu gaucgagggc gaccucaacc cugacaacag cgacgucgac aagcucuuca      600

uccagcucgu ccagaccuac aaccagcucu ucgaggagaa ccccaucaac gccagcggcg      660

ucgacgccaa ggccauccuc agcgcccgcc ucagcaagag ccgccgccuc gagaaucuca      720

ucgcccagcu uccaggugag aagaagaaug ggcucuucgg caaucucauc gcacucagcc      780

ucggccucac ucccaacuuc aagagcaacu ucgaccucgc ggaggacgcc aagcuccagc      840

ucagcaagga caccuacgac gacgaccucg acaaucuccu cgcccagauc ggcgaccagu      900

acgccgaccu cuuccuggcu gccaagaauc ucagcgacgc cauccuccuc agcgacaucc      960

ugcgggucaa cacagagauc accaaggccc cccucagcgc cagcaugaua aagcgcuacg     1020

acgagcacca ccaggaccuc acccuccuca aggcacuggu ccgccagcag cuuccagaga     1080

aguacaagga gaucuucuuc gaccagagca agaaugggua cgccggguac aucgacggug     1140

gugccagcca ggaggaguuc uacaaguuca ucaagcccau ccucgagaag auggacggca     1200

cagaggagcu gcucgucaag cucaacaggg aggaccuccu gcggaagcag cgcaccuucg     1260

acaaugggag caucccccac cagauccacc ucggugagcu gcacgccauc cugcggcgcc     1320

aggaggacuu cuaccccuuc cugaaggaca acagggagaa gaucgagaag auccucaccu     1380

uccgcauccc cuacuacguu ggcccccucg cccgcggcaa cagccgcuuc gccuggauga     1440

cccgcaagag cgaggagacc aucacucccu ggaacuucga ggaagucguc gacaagggug     1500

ccagcgccca gagcuucauc gagcgcauga ccaacuucga caagaaucuu ccaaacgaga     1560

agguccuucc aaagcacagc cuccucuacg aguacuucac cgucuacaac gagcugacca     1620

aggucaagua cgucacagag ggcaugcgca agccagccuu ccucagcggu gagcagaaga     1680

aggccaucgu cgaccuccuc uucaagacca accgcaaggu caccgucaag cagcucaagg     1740

aggacuacuu caagaagauc gagugcuucg acagcgucga gaucagcggc gucgaggacc     1800

gcuucaacgc cagccucggc accuaccacg accuccucaa gaucaucaag gacaaggacu     1860

uccuggacaa cgaggagaac gaggacaucc ucgaggacau cguccucacc cucacccucu     1920

ucgaggacag ggagaugaua gaggagcgcc ucaagaccua cgcccaccuc uucgacgaca     1980

aggucaugaa gcagcucaag cgccgccgcu acaccggcug gggccgccuc agccgcaagc     2040

ucaucaaugg gauccgagac aagcagagcg gcaagaccau ccucgacuuc cugaagagcg     2100

acggcuucgc caaccgcaac uucaugcagc ucauccacga cgacagccuc accuucaagg     2160

aggacaucca gaaggcccag gucagcggcc agggcgacag ccuccacgag cacaucgcca     2220

aucucgccgg gagcccagcc aucaagaagg ggauccucca gaccgucaag gucgucgacg     2280

agcuggucaa ggucaugggc cgccacaagc cagagaacau cgucaucgag auggccaggg     2340

agaaccagac cacucaaaag gggcagaaga acagcaggga gcgcaugaag cgcaucgagg     2400

agggcaucaa ggagcugggc agccagaucc ucaaggagca ccccgucgag aacacucaac     2460

uccagaacga gaagcucuac cucuacuacc uccagaaugg gcgagacaug uacgucgacc     2520

aggagcugga caucaaccgc cucagcgacu acgacgucga ccacaucguu ccccagagcu     2580

uccugaagga cgacagcauc gacaacaagg uccucacccg aagcgacaag aaccgcggca     2640

agagcgacaa cguucccuca gaggaagucg ucaagaagau gaagaacuac uggcgccagc     2700

uccucaacgc caagcucauc acucaacgca aguucgacaa ucucaccaag gcggagcgcg     2760

guggccucag cgagcuggac aaggccgggu ucaucaagcg ccagcucguc gagacccgcc     2820

agaucaccaa gcacgucgcc cagauccucg acagccgcau gaacaccaag uacgacgaga     2880

acgacaagcu caucagggaa gucaagguca ucacccucaa gagcaagcuc gucagcgacu     2940

uccgcaagga cuuccaguuc uacaagguca gggagaucaa caacuaccac cacgcccacg     3000

acgccuaccu caacgcugug guuggcaccg cacugaucaa gaaguacccc aagcucgaga     3060

gcgaguucgu cuacggcgac uacaaggucu acgacguccg caagaugaua gccaagagcg     3120

agcaggagau cggcaaggcc accgccaagu acuucuucua cagcaacauc augaacuucu     3180

ucaagacaga gaucacccuc gccaauggug agauccgcaa gcgcccccuc aucgagacca     3240

auggugagac cggugagauc gucugggaca aggggcgaga cuucgccacc guccgcaagg     3300

uccucagcau gccccaggug aacaucguca agaagacaga aguccagacc gguggcuuca     3360

gcaaggagag cauccuucca aagcgcaaca gcgacaagcu caucgcccgc aagaaggacu     3420

gggaccccaa gaaguacggu ggcuucgaca gccccaccgu cgccuacagc guccucgucg     3480

ucgccaaggu cgagaagggg aagagcaaga agcucaagag cgucaaggag cugcucggca     3540

ucaccaucau ggagcgaagc agcuucgaga agaaccccau cgacuuccug gaagccaagg     3600

gguacaagga agucaagaag gaccucauca ucaagcuucc aaaguacagc cucuucgagc     3660

uggagaaugg gcgcaagcgc augcucgcca gcgccgguga gcugcagaag gggaacgagc     3720

uggcacuucc cucaaaguac gucaacuucc uguaccucgc cagccacuac gagaagcuca     3780

aggggagccc agaggacaac gagcagaagc agcucuucgu cgagcagcac aagcacuacc     3840

ucgacgagau caucgagcag aucagcgagu ucagcaagcg cgucauccuc gccgacgcca     3900

aucucgacaa gguccucagc gccuacaaca agcaccgaga caagcccauc agggagcagg     3960

ccgagaacau cauccaccuc uucacccuca ccaaucucgg ugccccagcu gccuucaagu     4020

acuucgacac caccaucgac cgcaagcgcu acaccucgac uaaggaaguc cucgacgcca     4080

cccucaucca ccagagcauc accggccucu acgagacccg caucgaccuc agccagcucg     4140

guggcgacgg ugguggcagc cccaagaaga agcgcaaggu cuagcuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucuc     4320

ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ucuag                     4425


<210>  18
<211>  4425
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript comprising SEQ 8

<400>  18
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguaca       60

gcaucggccu ggacaucggc acgaacagcg uuggcugggc ugugaucacg gacgaguaca      120

agguucccuc aaagaaguuc aaggugcugg gcaacacgga ccggcacagc aucaagaaga      180

aucucaucgg ugcacugcug uucgacagcg gugagacggc cgaagccacg cggcugaagc      240

ggacggcccg ccggcgguac acgcggcgga agaaccggau cugcuaccug caggagaucu      300

ucagcaacga gauggccaag guggacgaca gcuucuucca ccggcuggag gagagcuucc      360

ugguggagga ggacaagaag cacgagcggc accccaucuu cggcaacauc guggacgaag      420

ucgccuacca cgagaaguac cccaccaucu accaccugcg gaagaagcug guggacucga      480

cugacaaggc cgaccugcgg cugaucuacc uggcacuggc ccacaugaua aaguuccggg      540

gccacuuccu gaucgagggc gaccugaacc cugacaacag cgacguggac aagcuguuca      600

uccagcuggu gcagaccuac aaccagcugu ucgaggagaa ccccaucaac gccagcggcg      660

uggacgccaa ggccauccuc agcgcccgcc ucagcaagag ccggcggcug gagaaucuca      720

ucgcccagcu uccaggugag aagaagaaug ggcuguucgg caaucucauc gcacucagcc      780

ugggccugac ucccaacuuc aagagcaacu ucgaccuggc cgaggacgcc aagcugcagc      840

ucagcaagga caccuacgac gacgaccugg acaaucuccu ggcccagauc ggcgaccagu      900

acgccgaccu guuccuggcu gccaagaauc ucagcgacgc cauccugcuc agcgacaucc      960

ugcgggugaa cacagagauc acgaaggccc cccucagcgc cagcaugaua aagcgguacg     1020

acgagcacca ccaggaccug acgcugcuga aggcacuggu gcggcagcag cuuccagaga     1080

aguacaagga gaucuucuuc gaccagagca agaaugggua cgccggguac aucgacggug     1140

gugccagcca ggaggaguuc uacaaguuca ucaagcccau ccuggagaag auggacggca     1200

cagaggagcu gcuggugaag cugaacaggg aggaccugcu gcggaagcag cggacguucg     1260

acaaugggag caucccccac cagauccacc ugggugagcu gcacgccauc cugcggcggc     1320

aggaggacuu cuaccccuuc cugaaggaca acagggagaa gaucgagaag auccugacgu     1380

uccggauccc cuacuacguu ggcccccugg cccgcggcaa cagccgguuc gccuggauga     1440

cgcggaagag cgaggagacg aucacucccu ggaacuucga ggaagucgug gacaagggug     1500

ccagcgccca gagcuucauc gagcggauga cgaacuucga caagaaucuu ccaaacgaga     1560

aggugcuucc aaagcacagc cugcuguacg aguacuucac gguguacaac gagcugacga     1620

aggugaagua cgugacagag ggcaugcgga agcccgccuu ccucagcggu gagcagaaga     1680

aggccaucgu ggaccugcug uucaagacga accggaaggu gacggugaag cagcugaagg     1740

aggacuacuu caagaagauc gagugcuucg acagcgugga gaucagcggc guggaggacc     1800

gguucaacgc cagccugggc accuaccacg accugcugaa gaucaucaag gacaaggacu     1860

uccuggacaa cgaggagaac gaggacaucc uggaggacau cgugcugacg cugacgcugu     1920

ucgaggacag ggagaugaua gaggagcggc ugaagaccua cgcccaccug uucgacgaca     1980

aggugaugaa gcagcugaag cggcggcggu acacgggcug gggccggcuc agccggaagc     2040

ugaucaaugg gauccgagac aagcagagcg gcaagacgau ccuggacuuc cugaagagcg     2100

acggcuucgc caaccggaac uucaugcagc ugauccacga cgacagccug acguucaagg     2160

aggacaucca gaaggcccag gucagcggcc agggcgacag ccugcacgag cacaucgcca     2220

aucucgccgg gagccccgcc aucaagaagg ggauccugca gacggugaag gugguggacg     2280

agcuggugaa ggugaugggc cggcacaagc cagagaacau cgugaucgag auggccaggg     2340

agaaccagac gacucaaaag gggcagaaga acagcaggga gcggaugaag cggaucgagg     2400

agggcaucaa ggagcugggc agccagaucc ugaaggagca ccccguggag aacacucaac     2460

ugcagaacga gaagcuguac cuguacuacc ugcagaaugg gcgagacaug uacguggacc     2520

aggagcugga caucaaccgg cucagcgacu acgacgugga ccacaucguu ccccagagcu     2580

uccugaagga cgacagcauc gacaacaagg ugcugacgcg gagcgacaag aaccggggca     2640

agagcgacaa cguucccuca gaggaagucg ugaagaagau gaagaacuac uggcggcagc     2700

ugcugaacgc caagcugauc acucaacgga aguucgacaa ucucacgaag gccgagcggg     2760

guggccucag cgagcuggac aaggccgggu ucaucaagcg gcagcuggug gagacgcggc     2820

agaucacgaa gcacguggcc cagauccugg acagccggau gaacacgaag uacgacgaga     2880

acgacaagcu gaucagggaa gucaagguga ucacgcugaa gagcaagcug gucagcgacu     2940

uccggaagga cuuccaguuc uacaagguga gggagaucaa caacuaccac cacgcccacg     3000

acgccuaccu gaacgcugug guuggcacgg cacugaucaa gaaguacccc aagcuggaga     3060

gcgaguucgu guacggcgac uacaaggugu acgacgugcg gaagaugaua gccaagagcg     3120

agcaggagau cggcaaggcc acggccaagu acuucuucua cagcaacauc augaacuucu     3180

ucaagacaga gaucacgcug gccaauggug agauccggaa gcggccccug aucgagacga     3240

auggugagac gggugagauc gugugggaca aggggcgaga cuucgccacg gugcggaagg     3300

ugcucagcau gccccaggug aacaucguga agaagacaga aguccagacg gguggcuuca     3360

gcaaggagag cauccuucca aagcggaaca gcgacaagcu gaucgcccgc aagaaggacu     3420

gggaccccaa gaaguacggu ggcuucgaca gccccaccgu ggccuacagc gugcuggugg     3480

uggccaaggu ggagaagggg aagagcaaga agcugaagag cgugaaggag cugcugggca     3540

ucacgaucau ggagcggagc agcuucgaga agaaccccau cgacuuccug gaagccaagg     3600

gguacaagga agucaagaag gaccugauca ucaagcuucc aaaguacagc cuguucgagc     3660

uggagaaugg gcggaagcgg augcuggcca gcgccgguga gcugcagaag gggaacgagc     3720

uggcacuucc cucaaaguac gugaacuucc uguaccuggc cagccacuac gagaagcuga     3780

aggggagccc agaggacaac gagcagaagc agcuguucgu ggagcagcac aagcacuacc     3840

uggacgagau caucgagcag aucagcgagu ucagcaagcg ggugauccug gccgacgcca     3900

aucucgacaa ggugcucagc gccuacaaca agcaccgaga caagcccauc agggagcagg     3960

ccgagaacau cauccaccug uucacgcuga cgaaucucgg ugcccccgcu gccuucaagu     4020

acuucgacac gacgaucgac cggaagcggu acacgucgac uaaggaaguc cuggacgcca     4080

cgcugaucca ccagagcauc acgggccugu acgagacgcg gaucgaccuc agccagcugg     4140

guggcgacgg ugguggcagc cccaagaaga agcggaaggu guagcuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucuc     4320

ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ucuag                     4425


<210>  19
<211>  4434
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 mRNA transcript comprising SEQ 10

<400>  19
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguaca       60

gcaucggccu cgacaucggc accaacagcg ucggcugggc cgucaucacc gacgaguaca      120

agguccccag caagaaguuc aagguccucg gcaacaccga ccgccacagc aucaagaaga      180

accucaucgg cgcccuccuc uucgacagcg gcgagaccgc cgaggccacc cgccucaagc      240

gcaccgcccg ccgccgcuac acccgccgca agaaccgcau cugcuaccuc caggagaucu      300

ucagcaacga gauggccaag gucgacgaca gcuucuucca ccgccucgag gagagcuucc      360

ucgucgagga ggacaagaag cacgagcgcc accccaucuu cggcaacauc gucgacgagg      420

ucgccuacca cgagaaguac cccaccaucu accaccuccg caagaagcuc gucgacagca      480

ccgacaaggc cgaccuccgc cucaucuacc ucgcccucgc ccacaugauc aaguuccgcg      540

gccacuuccu caucgagggc gaccucaacc ccgacaacag cgacgucgac aagcucuuca      600

uccagcucgu ccagaccuac aaccagcucu ucgaggagaa ccccaucaac gccagcggcg      660

ucgacgccaa ggccauccuc agcgcccgcc ucagcaagag ccgccgccuc gagaaccuca      720

ucgcccagcu ccccggcgag aagaagaacg gccucuucgg caaccucauc gcccucagcc      780

ucggccucac ccccaacuuc aagagcaacu ucgaccucgc cgaggacgcc aagcuccagc      840

ucagcaagga caccuacgac gacgaccucg acaaccuccu cgcccagauc ggcgaccagu      900

acgccgaccu cuuccucgcc gccaagaacc ucagcgacgc cauccuccuc agcgacaucc      960

uccgcgucaa caccgagauc accaaggccc cccucagcgc cagcaugauc aagcgcuacg     1020

acgagcacca ccaggaccuc acccuccuca aggcccucgu ccgccagcag cuccccgaga     1080

aguacaagga gaucuucuuc gaccagagca agaacggcua cgccggcuac aucgacggcg     1140

gcgccagcca ggaggaguuc uacaaguuca ucaagcccau ccucgagaag auggacggca     1200

ccgaggagcu ccucgucaag cucaaccgcg aggaccuccu ccgcaagcag cgcaccuucg     1260

acaacggcag caucccccac cagauccacc ucggcgagcu ccacgccauc cuccgccgcc     1320

aggaggacuu cuaccccuuc cucaaggaca accgcgagaa gaucgagaag auccucaccu     1380

uccgcauccc cuacuacguc ggcccccucg cccgcggcaa cagccgcuuc gccuggauga     1440

cccgcaagag cgaggagacc aucacccccu ggaacuucga ggaggucguc gacaagggcg     1500

ccagcgccca gagcuucauc gagcgcauga ccaacuucga caagaaccuc cccaacgaga     1560

agguccuccc caagcacagc cuccucuacg aguacuucac cgucuacaac gagcucacca     1620

aggucaagua cgucaccgag ggcaugcgca agcccgccuu ccucagcggc gagcagaaga     1680

aggccaucgu cgaccuccuc uucaagacca accgcaaggu caccgucaag cagcucaagg     1740

aggacuacuu caagaagauc gagugcuucg acagcgucga gaucagcggc gucgaggacc     1800

gcuucaacgc cagccucggc accuaccacg accuccucaa gaucaucaag gacaaggacu     1860

uccucgacaa cgaggagaac gaggacaucc ucgaggacau cguccucacc cucacccucu     1920

ucgaggaccg cgagaugauc gaggagcgcc ucaagaccua cgcccaccuc uucgacgaca     1980

aggucaugaa gcagcucaag cgccgccgcu acaccggcug gggccgccuc agccgcaagc     2040

ucaucaacgg cauccgcgac aagcagagcg gcaagaccau ccucgacuuc cucaagagcg     2100

acggcuucgc caaccgcaac uucaugcagc ucauccacga cgacagccuc accuucaagg     2160

aggacaucca gaaggcccag gucagcggcc agggcgacag ccuccacgag cacaucgcca     2220

accucgccgg cagccccgcc aucaagaagg gcauccucca gaccgucaag gucgucgacg     2280

agcucgucaa ggucaugggc cgccacaagc ccgagaacau cgucaucgag auggcccgcg     2340

agaaccagac cacccagaag ggccagaaga acagccgcga gcgcaugaag cgcaucgagg     2400

agggcaucaa ggagcucggc agccagaucc ucaaggagca ccccgucgag aacacccagc     2460

uccagaacga gaagcucuac cucuacuacc uccagaacgg ccgcgacaug uacgucgacc     2520

aggagcucga caucaaccgc cucagcgacu acgacgucga ccacaucguc ccccagagcu     2580

uccucaagga cgacagcauc gacaacaagg uccucacccg cagcgacaag aaccgcggca     2640

agagcgacaa cguccccagc gaggaggucg ucaagaagau gaagaacuac uggcgccagc     2700

uccucaacgc caagcucauc acccagcgca aguucgacaa ccucaccaag gccgagcgcg     2760

gcggccucag cgagcucgac aaggccggcu ucaucaagcg ccagcucguc gagacccgcc     2820

agaucaccaa gcacgucgcc cagauccucg acagccgcau gaacaccaag uacgacgaga     2880

acgacaagcu cauccgcgag gucaagguca ucacccucaa gagcaagcuc gucagcgacu     2940

uccgcaagga cuuccaguuc uacaaggucc gcgagaucaa caacuaccac cacgcccacg     3000

acgccuaccu caacgccguc gucggcaccg cccucaucaa gaaguacccc aagcucgaga     3060

gcgaguucgu cuacggcgac uacaaggucu acgacguccg caagaugauc gccaagagcg     3120

agcaggagau cggcaaggcc accgccaagu acuucuucua cagcaacauc augaacuucu     3180

ucaagaccga gaucacccuc gccaacggcg agauccgcaa gcgcccccuc aucgagacca     3240

acggcgagac cggcgagauc gucugggaca agggccgcga cuucgccacc guccgcaagg     3300

uccucagcau gccccagguc aacaucguca agaagaccga gguccagacc ggcggcuuca     3360

gcaaggagag cauccucccc aagcgcaaca gcgacaagcu caucgcccgc aagaaggacu     3420

gggaccccaa gaaguacggc ggcuucgaca gccccaccgu cgccuacagc guccucgucg     3480

ucgccaaggu cgagaagggc aagagcaaga agcucaagag cgucaaggag cuccucggca     3540

ucaccaucau ggagcgcagc agcuucgaga agaaccccau cgacuuccuc gaggccaagg     3600

gcuacaagga ggucaagaag gaccucauca ucaagcuccc caaguacagc cucuucgagc     3660

ucgagaacgg ccgcaagcgc augcucgcca gcgccggcga gcuccagaag ggcaacgagc     3720

ucgcccuccc cagcaaguac gucaacuucc ucuaccucgc cagccacuac gagaagcuca     3780

agggcagccc cgaggacaac gagcagaagc agcucuucgu cgagcagcac aagcacuacc     3840

ucgacgagau caucgagcag aucagcgagu ucagcaagcg cgucauccuc gccgacgcca     3900

accucgacaa gguccucagc gccuacaaca agcaccgcga caagcccauc cgcgagcagg     3960

ccgagaacau cauccaccuc uucacccuca ccaaccucgg cgcccccgcc gccuucaagu     4020

acuucgacac caccaucgac cgcaagcgcu acaccagcac caaggagguc cucgacgcca     4080

cccucaucca ccagagcauc accggccucu acgagacccg caucgaccuc agccagcucg     4140

gcggcgacgg cggcggcagc cccaagaaga agcgcaaggu cuagcuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucuc     4320

ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau cuag           4434


<210>  20
<211>  4431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript comprising SEQ 6

<400>  20
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguaca       60

gcaucggccu ggacaucggc acgaacagcg uuggcugggc ugugaucacg gacgaguaca      120

agguucccag caagaaguuc aaggugcugg gcaacacgga ccggcacagc aucaagaaga      180

aucugaucgg ugcacugcug uucgacagcg gugagacggc cgaagccacg cggcugaagc      240

ggacggcccg gcggcgguac acgcggcgga agaaccggau cugcuaccug caggagaucu      300

ucagcaacga gauggccaag guggacgaca gcuucuucca ccggcuggag gagagcuucc      360

ugguggagga ggacaagaag cacgagcggc accccaucuu cggcaacauc guggacgaag      420

uggccuacca cgagaaguac cccacgaucu accaccugcg gaagaagcug guggacucga      480

cggacaaggc cgaccugcgg cugaucuacc uggcacuggc ccacaugauc aaguuccggg      540

gccacuuccu gaucgagggc gaccugaacc cugacaacag cgacguggac aagcuguuca      600

uccagcuggu gcagaccuac aaccagcugu ucgaggagaa ccccaucaac gccagcggcg      660

uggacgccaa ggccauccuc agcgcccggc ucagcaagag ccggcggcug gagaaucuga      720

ucgcccagcu ucccggugag aagaagaaug gccuguucgg caaucugauc gcacucagcc      780

ugggccugac ucccaacuuc aagagcaacu ucgaccuggc cgaggacgcc aagcugcagc      840

ucagcaagga caccuacgac gacgaccugg acaaucugcu ggcccagauc ggcgaccagu      900

acgccgaccu guuccuggcu gccaagaauc ucagcgacgc cauccugcuc agcgacaucc      960

ugcgggugaa cacagagauc acgaaggccc cccucagcgc cagcaugauc aagcgguacg     1020

acgagcacca ccaggaccug acgcugcuga aggcacuggu gcggcagcag cuuccagaga     1080

aguacaagga gaucuucuuc gaccagagca agaauggcua cgccggcuac aucgacggug     1140

gugccagcca ggaggaguuc uacaaguuca ucaagcccau ccuggagaag auggacggca     1200

cagaggagcu gcuggugaag cugaacaggg aggaccugcu gcggaagcag cggacguucg     1260

acaauggcag caucccccac cagauccacc ugggugagcu gcacgccauc cugcggcggc     1320

aggaggacuu cuaccccuuc cugaaggaca acagggagaa gaucgagaag auccugacgu     1380

uccggauccc cuacuacguu ggcccccugg cccggggcaa cagccgguuc gccuggauga     1440

cgcggaagag cgaggagacg aucacucccu ggaacuucga ggaaguggug gacaagggug     1500

ccagcgccca gagcuucauc gagcggauga cgaacuucga caagaaucuu cccaacgaga     1560

aggugcuucc caagcacagc cugcuguacg aguacuucac gguguacaac gagcugacga     1620

aggugaagua cgugacagag ggcaugcgga agcccgccuu ccucagcggu gagcagaaga     1680

aggccaucgu ggaccugcug uucaagacga accggaaggu gacggugaag cagcugaagg     1740

aggacuacuu caagaagauc gagugcuucg acagcgugga gaucagcggc guggaggacc     1800

gguucaacgc cagccugggc accuaccacg accugcugaa gaucaucaag gacaaggacu     1860

uccuggacaa cgaggagaac gaggacaucc uggaggacau cgugcugacg cugacgcugu     1920

ucgaggacag ggagaugauc gaggagcggc ugaagaccua cgcccaccug uucgacgaca     1980

aggugaugaa gcagcugaag cggcggcggu acacgggcug gggccggcuc agccggaagc     2040

ugaucaaugg cauccgagac aagcagagcg gcaagacgau ccuggacuuc cugaagagcg     2100

acggcuucgc caaccggaac uucaugcagc ugauccacga cgacagccug acguucaagg     2160

aggacaucca gaaggcccag gucagcggcc agggcgacag ccugcacgag cacaucgcca     2220

aucuggccgg cagccccgcc aucaagaagg gcauccugca gacggugaag gugguggacg     2280

agcuggugaa ggugaugggc cggcacaagc cagagaacau cgugaucgag auggccaggg     2340

agaaccagac gacucagaag ggccagaaga acagcaggga gcggaugaag cggaucgagg     2400

agggcaucaa ggagcugggc agccagaucc ugaaggagca ccccguggag aacacucagc     2460

ugcagaacga gaagcuguac cuguacuacc ugcagaaugg ccgagacaug uacguggacc     2520

aggagcugga caucaaccgg cucagcgacu acgacgugga ccacaucguu ccccagagcu     2580

uccugaagga cgacagcauc gacaacaagg ugcugacgcg gagcgacaag aaccggggca     2640

agagcgacaa cguucccagc gaggaagugg ugaagaagau gaagaacuac uggcggcagc     2700

ugcugaacgc caagcugauc acucagcgga aguucgacaa ucugacgaag gccgagcggg     2760

guggccucag cgagcuggac aaggccggcu ucaucaagcg gcagcuggug gagacgcggc     2820

agaucacgaa gcacguggcc cagauccugg acagccggau gaacacgaag uacgacgaga     2880

acgacaagcu gaucagggaa gugaagguga ucacgcugaa gagcaagcug gucagcgacu     2940

uccggaagga cuuccaguuc uacaagguga gggagaucaa caacuaccac cacgcccacg     3000

acgccuaccu gaacgcugug guuggcacgg cacugaucaa gaaguacccc aagcuggaga     3060

gcgaguucgu guacggcgac uacaaggugu acgacgugcg gaagaugauc gccaagagcg     3120

agcaggagau cggcaaggcc acggccaagu acuucuucua cagcaacauc augaacuucu     3180

ucaagacaga gaucacgcug gccaauggug agauccggaa gcggccccug aucgagacga     3240

auggugagac gggugagauc gugugggaca agggccgaga cuucgccacg gugcggaagg     3300

ugcucagcau gccccaggug aacaucguga agaagacaga agugcagacg gguggcuuca     3360

gcaaggagag cauccuuccc aagcggaaca gcgacaagcu gaucgcccgg aagaaggacu     3420

gggaccccaa gaaguacggu ggcuucgaca gccccacggu ggccuacagc gugcuggugg     3480

uggccaaggu ggagaagggc aagagcaaga agcugaagag cgugaaggag cugcugggca     3540

ucacgaucau ggagcggagc agcuucgaga agaaccccau cgacuuccug gaagccaagg     3600

gcuacaagga agugaagaag gaccugauca ucaagcuucc caaguacagc cuguucgagc     3660

uggagaaugg ccggaagcgg augcuggcca gcgccgguga gcugcagaag ggcaacgagc     3720

uggcacuucc cagcaaguac gugaacuucc uguaccuggc cagccacuac gagaagcuga     3780

agggcagccc agaggacaac gagcagaagc agcuguucgu ggagcagcac aagcacuacc     3840

uggacgagau caucgagcag aucagcgagu ucagcaagcg ggugauccug gccgacgcca     3900

aucuggacaa ggugcucagc gccuacaaca agcaccgaga caagcccauc agggagcagg     3960

ccgagaacau cauccaccug uucacgcuga cgaaucuggg ugcccccgcu gccuucaagu     4020

acuucgacac gacgaucgac cggaagcggu acacgucgac gaaggaagug cuggacgcca     4080

cgcugaucca ccagagcauc acgggccugu acgagacgcg gaucgaccuc agccagcugg     4140

guggcgacgg ugguggcagc cccaagaaga agcggaaggu guagcuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucuc     4320

ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaucua g              4431


<210>  21
<211>  4430
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 mRNA transcript comprising SEQ 11

<400>  21
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aaaaaauacu       60

caauaggccu cgacauaggc accaacucag ucggcugggc cgucauaacc gacgaguaca      120

aaguccccuc aaaaaaauuc aaaguccucg gcaacaccga caggcacuca auaaaaaaaa      180

accucauagg cgcccuccuc uucgacucag gcgagaccgc cgaggccacc aggcucaaaa      240

ggaccgccag gaggagguac accaggagga aaaacaggau augcuaccuc caggagauau      300

ucucaaacga gauggccaaa gucgacgacu cauucuucca caggcucgag gagucauucc      360

ucgucgagga ggacaaaaaa cacgagaggc accccauauu cggcaacaua gucgacgagg      420

ucgccuacca cgagaaauac cccaccauau accaccucag gaaaaaacuc gucgacucaa      480

ccgacaaagc cgaccucagg cucauauacc ucgcccucgc ccacaugaua aaauucaggg      540

gccacuuccu cauagagggc gaccucaacc ccgacaacuc agacgucgac aaacucuuca      600

uacagcucgu ccagaccuac aaccagcucu ucgaggagaa ccccauaaac gccucaggcg      660

ucgacgccaa agccauacuc ucagccaggc ucucaaaauc aaggaggcuc gagaaccuca      720

uagcccagcu ccccggcgag aaaaaaaacg gccucuucgg caaccucaua gcccucucac      780

ucggccucac ccccaacuuc aaaucaaacu ucgaccucgc cgaggacgcc aaacuccagc      840

ucucaaaaga caccuacgac gacgaccucg acaaccuccu cgcccagaua ggcgaccagu      900

acgccgaccu cuuccucgcc gccaaaaacc ucucagacgc cauacuccuc ucagacauac      960

ucagggucaa caccgagaua accaaagccc cccucucagc cucaaugaua aaaagguacg     1020

acgagcacca ccaggaccuc acccuccuca aagcccucgu caggcagcag cuccccgaga     1080

aauacaaaga gauauucuuc gaccagucaa aaaacggcua cgccggcuac auagacggcg     1140

gcgccucaca ggaggaguuc uacaaauuca uaaaacccau acucgagaaa auggacggca     1200

ccgaggagcu ccucgucaaa cucaacaggg aggaccuccu caggaaacag aggaccuucg     1260

acaacggcuc aauaccccac cagauacacc ucggcgagcu ccacgccaua cucaggaggc     1320

aggaggacuu cuaccccuuc cucaaagaca acagggagaa aauagagaaa auacucaccu     1380

ucaggauacc cuacuacguc ggcccccucg ccaggggcaa cucaagguuc gccuggauga     1440

ccaggaaauc agaggagacc auaacccccu ggaacuucga ggaggucguc gacaaaggcg     1500

ccucagccca gucauucaua gagaggauga ccaacuucga caaaaaccuc cccaacgaga     1560

aaguccuccc caaacacuca cuccucuacg aguacuucac cgucuacaac gagcucacca     1620

aagucaaaua cgucaccgag ggcaugagga aacccgccuu ccucucaggc gagcagaaaa     1680

aagccauagu cgaccuccuc uucaaaacca acaggaaagu caccgucaaa cagcucaaag     1740

aggacuacuu caaaaaaaua gagugcuucg acucagucga gauaucaggc gucgaggaca     1800

gguucaacgc cucacucggc accuaccacg accuccucaa aauaauaaaa gacaaagacu     1860

uccucgacaa cgaggagaac gaggacauac ucgaggacau aguccucacc cucacccucu     1920

ucgaggacag ggagaugaua gaggagaggc ucaaaaccua cgcccaccuc uucgacgaca     1980

aagucaugaa acagcucaaa aggaggaggu acaccggcug gggcaggcuc ucaaggaaac     2040

ucauaaacgg cauaagggac aaacagucag gcaaaaccau acucgacuuc cucaaaucag     2100

acggcuucgc caacaggaac uucaugcagc ucauacacga cgacucacuc accuucaaag     2160

aggacauaca gaaagcccag gucucaggcc agggcgacuc acuccacgag cacauagcca     2220

accucgccgg cucacccgcc auaaaaaaag gcauacucca gaccgucaaa gucgucgacg     2280

agcucgucaa agucaugggc aggcacaaac ccgagaacau agucauagag auggccaggg     2340

agaaccagac cacccagaaa ggccagaaaa acucaaggga gaggaugaaa aggauagagg     2400

agggcauaaa agagcucggc ucacagauac ucaaagagca ccccgucgag aacacccagc     2460

uccagaacga gaaacucuac cucuacuacc uccagaacgg cagggacaug uacgucgacc     2520

aggagcucga cauaaacagg cucucagacu acgacgucga ccacauaguc ccccagucau     2580

uccucaaaga cgacucaaua gacaacaaag uccucaccag gucagacaaa aacaggggca     2640

aaucagacaa cguccccuca gaggaggucg ucaaaaaaau gaaaaacuac uggaggcagc     2700

uccucaacgc caaacucaua acccagagga aauucgacaa ccucaccaaa gccgagaggg     2760

gcggccucuc agagcucgac aaagccggcu ucauaaaaag gcagcucguc gagaccaggc     2820

agauaaccaa acacgucgcc cagauacucg acucaaggau gaacaccaaa uacgacgaga     2880

acgacaaacu cauaagggag gucaaaguca uaacccucaa aucaaaacuc gucucagacu     2940

ucaggaaaga cuuccaguuc uacaaaguca gggagauaaa caacuaccac cacgcccacg     3000

acgccuaccu caacgccguc gucggcaccg cccucauaaa aaaauacccc aaacucgagu     3060

cagaguucgu cuacggcgac uacaaagucu acgacgucag gaaaaugaua gccaaaucag     3120

agcaggagau aggcaaagcc accgccaaau acuucuucua cucaaacaua augaacuucu     3180

ucaaaaccga gauaacccuc gccaacggcg agauaaggaa aaggccccuc auagagacca     3240

acggcgagac cggcgagaua gucugggaca aaggcaggga cuucgccacc gucaggaaag     3300

uccucucaau gccccagguc aacauaguca aaaaaaccga gguccagacc ggcggcuucu     3360

caaaagaguc aauacucccc aaaaggaacu cagacaaacu cauagccagg aaaaaagacu     3420

gggaccccaa aaaauacggc ggcuucgacu cacccaccgu cgccuacuca guccucgucg     3480

ucgccaaagu cgagaaaggc aaaucaaaaa aacucaaauc agucaaagag cuccucggca     3540

uaaccauaau ggagagguca ucauucgaga aaaaccccau agacuuccuc gaggccaaag     3600

gcuacaaaga ggucaaaaaa gaccucauaa uaaaacuccc caaauacuca cucuucgagc     3660

ucgagaacgg caggaaaagg augcucgccu cagccggcga gcuccagaaa ggcaacgagc     3720

ucgcccuccc cucaaaauac gucaacuucc ucuaccucgc cucacacuac gagaaacuca     3780

aaggcucacc cgaggacaac gagcagaaac agcucuucgu cgagcagcac aaacacuacc     3840

ucgacgagau aauagagcag auaucagagu ucucaaaaag ggucauacuc gccgacgcca     3900

accucgacaa aguccucuca gccuacaaca aacacaggga caaacccaua agggagcagg     3960

ccgagaacau aauacaccuc uucacccuca ccaaccucgg cgcccccgcc gccuucaaau     4020

acuucgacac caccauagac aggaaaaggu acaccucaac caaagagguc cucgacgcca     4080

cccucauaca ccagucaaua accggccucu acgagaccag gauagaccuc ucacagcucg     4140

gcggcgacgg cggcggcuca cccaaaaaaa aaaggaaagu cuagcuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucuc     4320

ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaucuag                4430


<210>  22
<211>  4427
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 mRNA transcript comprising SEQ 12

<400>  22
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aaaaaauaca       60

gcaucggccu ggacaucggc acgaacagcg ugggcugggc cgugaucacg gacgaguaca      120

aagugcccag caaaaaauuc aaagugcugg gcaacacgga ccggcacagc aucaaaaaaa      180

accugaucgg cgcccugcug uucgacagcg gcgagacggc cgaggccacg cggcugaaac      240

ggacggcccg gcggcgguac acgcggcgga aaaaccggau cugcuaccug caggagaucu      300

ucagcaacga gauggccaaa guggacgaca gcuucuucca ccggcuggag gagagcuucc      360

ugguggagga ggacaaaaaa cacgagcggc accccaucuu cggcaacauc guggacgagg      420

uggccuacca cgagaaauac cccacgaucu accaccugcg gaaaaaacug guggacagca      480

cggacaaagc cgaccugcgg cugaucuacc uggcccuggc ccacaugauc aaauuccggg      540

gccacuuccu gaucgagggc gaccugaacc ccgacaacag cgacguggac aaacuguuca      600

uccagcuggu gcagacguac aaccagcugu ucgaggagaa ccccaucaac gccagcggcg      660

uggacgccaa agccauccug agcgcccggc ugagcaaaag ccggcggcug gagaaccuga      720

ucgcccagcu gcccggcgag aaaaaaaacg gccuguucgg caaccugauc gcccugagcc      780

ugggccugac gcccaacuuc aaaagcaacu ucgaccuggc cgaggacgcc aaacugcagc      840

ugagcaaaga cacguacgac gacgaccugg acaaccugcu ggcccagauc ggcgaccagu      900

acgccgaccu guuccuggcc gccaaaaacc ugagcgacgc cauccugcug agcgacaucc      960

ugcgggugaa cacggagauc acgaaagccc cccugagcgc cagcaugauc aaacgguacg     1020

acgagcacca ccaggaccug acgcugcuga aagcccuggu gcggcagcag cugcccgaga     1080

aauacaaaga gaucuucuuc gaccagagca aaaacggcua cgccggcuac aucgacggcg     1140

gcgccagcca ggaggaguuc uacaaauuca ucaaacccau ccuggagaaa auggacggca     1200

cggaggagcu gcuggugaaa cugaaccggg aggaccugcu gcggaaacag cggacguucg     1260

acaacggcag caucccccac cagauccacc ugggcgagcu gcacgccauc cugcggcggc     1320

aggaggacuu cuaccccuuc cugaaagaca accgggagaa aaucgagaaa auccugacgu     1380

uccggauccc cuacuacgug ggcccccugg cccggggcaa cagccgguuc gccuggauga     1440

cgcggaaaag cgaggagacg aucacgcccu ggaacuucga ggagguggug gacaaaggcg     1500

ccagcgccca gagcuucauc gagcggauga cgaacuucga caaaaaccug cccaacgaga     1560

aagugcugcc caaacacagc cugcuguacg aguacuucac gguguacaac gagcugacga     1620

aagugaaaua cgugacggag ggcaugcgga aacccgccuu ccugagcggc gagcagaaaa     1680

aagccaucgu ggaccugcug uucaaaacga accggaaagu gacggugaaa cagcugaaag     1740

aggacuacuu caaaaaaauc gagugcuucg acagcgugga gaucagcggc guggaggacc     1800

gguucaacgc cagccugggc acguaccacg accugcugaa aaucaucaaa gacaaagacu     1860

uccuggacaa cgaggagaac gaggacaucc uggaggacau cgugcugacg cugacgcugu     1920

ucgaggaccg ggagaugauc gaggagcggc ugaaaacgua cgcccaccug uucgacgaca     1980

aagugaugaa acagcugaaa cggcggcggu acacgggcug gggccggcug agccggaaac     2040

ugaucaacgg cauccgggac aaacagagcg gcaaaacgau ccuggacuuc cugaaaagcg     2100

acggcuucgc caaccggaac uucaugcagc ugauccacga cgacagccug acguucaaag     2160

aggacaucca gaaagcccag gugagcggcc agggcgacag ccugcacgag cacaucgcca     2220

accuggccgg cagccccgcc aucaaaaaag gcauccugca gacggugaaa gugguggacg     2280

agcuggugaa agugaugggc cggcacaaac ccgagaacau cgugaucgag auggcccggg     2340

agaaccagac gacgcagaaa ggccagaaaa acagccggga gcggaugaaa cggaucgagg     2400

agggcaucaa agagcugggc agccagaucc ugaaagagca ccccguggag aacacgcagc     2460

ugcagaacga gaaacuguac cuguacuacc ugcagaacgg ccgggacaug uacguggacc     2520

aggagcugga caucaaccgg cugagcgacu acgacgugga ccacaucgug ccccagagcu     2580

uccugaaaga cgacagcauc gacaacaaag ugcugacgcg gagcgacaaa aaccggggca     2640

aaagcgacaa cgugcccagc gaggaggugg ugaaaaaaau gaaaaacuac uggcggcagc     2700

ugcugaacgc caaacugauc acgcagcgga aauucgacaa ccugacgaaa gccgagcggg     2760

gcggccugag cgagcuggac aaagccggcu ucaucaaacg gcagcuggug gagacgcggc     2820

agaucacgaa acacguggcc cagauccugg acagccggau gaacacgaaa uacgacgaga     2880

acgacaaacu gauccgggag gugaaaguga ucacgcugaa aagcaaacug gugagcgacu     2940

uccggaaaga cuuccaguuc uacaaagugc gggagaucaa caacuaccac cacgcccacg     3000

acgccuaccu gaacgccgug gugggcacgg cccugaucaa aaaauacccc aaacuggaga     3060

gcgaguucgu guacggcgac uacaaagugu acgacgugcg gaaaaugauc gccaaaagcg     3120

agcaggagau cggcaaagcc acggccaaau acuucuucua cagcaacauc augaacuucu     3180

ucaaaacgga gaucacgcug gccaacggcg agauccggaa acggccccug aucgagacga     3240

acggcgagac gggcgagauc gugugggaca aaggccggga cuucgccacg gugcggaaag     3300

ugcugagcau gccccaggug aacaucguga aaaaaacgga ggugcagacg ggcggcuuca     3360

gcaaagagag cauccugccc aaacggaaca gcgacaaacu gaucgcccgg aaaaaagacu     3420

gggaccccaa aaaauacggc ggcuucgaca gccccacggu ggccuacagc gugcuggugg     3480

uggccaaagu ggagaaaggc aaaagcaaaa aacugaaaag cgugaaagag cugcugggca     3540

ucacgaucau ggagcggagc agcuucgaga aaaaccccau cgacuuccug gaggccaaag     3600

gcuacaaaga ggugaaaaaa gaccugauca ucaaacugcc caaauacagc cuguucgagc     3660

uggagaacgg ccggaaacgg augcuggcca gcgccggcga gcugcagaaa ggcaacgagc     3720

uggcccugcc cagcaaauac gugaacuucc uguaccuggc cagccacuac gagaaacuga     3780

aaggcagccc cgaggacaac gagcagaaac agcuguucgu ggagcagcac aaacacuacc     3840

uggacgagau caucgagcag aucagcgagu ucagcaaacg ggugauccug gccgacgcca     3900

accuggacaa agugcugagc gccuacaaca aacaccggga caaacccauc cgggagcagg     3960

ccgagaacau cauccaccug uucacgcuga cgaaccuggg cgcccccgcc gccuucaaau     4020

acuucgacac gacgaucgac cggaaacggu acacgagcac gaaagaggug cuggacgcca     4080

cgcugaucca ccagagcauc acgggccugu acgagacgcg gaucgaccug agccagcugg     4140

gcggcgacgg cggcggcagc cccaaaaaaa aacggaaagu guagcuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucuc     4320

ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaucuag                   4427


<210>  23
<211>  4424
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript comprising SEQ 13

<400>  23
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggau aaaaaauauu       60

caauaggauu agauauagga acaaauucag uaggaugggc aguaauaaca gaugaauaua      120

aaguaccauc aaaaaaauuu aaaguauuag gaaauacaga uagacauuca auaaaaaaaa      180

auuuaauagg agcauuauua uuugauucag gagaaacagc agaagcaaca agauuaaaaa      240

gaacagcaag aagaagauau acaagaagaa aaaauagaau auguuauuua caagaaauau      300

uuucaaauga aauggcaaaa guagaugauu cauuuuuuca uagauuagaa gaaucauuuu      360

uaguagaaga agauaaaaaa caugaaagac auccaauauu uggaaauaua guagaugaag      420

uagcauauca ugaaaaauau ccaacaauau aucauuuaag aaaaaaauua guagauucaa      480

cagauaaagc agauuuaaga uuaauauauu uagcauuagc acauaugaua aaauuuagag      540

gacauuuuuu aauagaagga gauuuaaauc cagauaauuc agauguagau aaauuauuua      600

uacaauuagu acaaacauau aaucaauuau uugaagaaaa uccaauaaau gcaucaggag      660

uagaugcaaa agcaauauua ucagcaagau uaucaaaauc aagaagauua gaaaauuuaa      720

uagcacaauu accaggagaa aaaaaaaaug gauuauuugg aaauuuaaua gcauuaucau      780

uaggauuaac accaaauuuu aaaucaaauu uugauuuagc agaagaugca aaauuacaau      840

uaucaaaaga uacauaugau gaugauuuag auaauuuauu agcacaaaua ggagaucaau      900

augcagauuu auuuuuagca gcaaaaaauu uaucagaugc aauauuauua ucagauauau      960

uaagaguaaa uacagaaaua acaaaagcac cauuaucagc aucaaugaua aaaagauaug     1020

augaacauca ucaggacuua acauuauuaa aagcauuagu aagacaacaa uuaccagaaa     1080

aauauaaaga aauauuuuuu gaucaaucaa aaaauggaua ugcaggauau auagauggag     1140

gagcaucaca agaagaauuu uauaaauuua uaaaaccaau auuagaaaaa auggauggaa     1200

cagaagaauu auuaguaaaa uuaaauagag aagauuuauu aagaaaacaa agaacauuug     1260

auaauggauc aauaccacau caaauacauu uaggagaauu acaugcaaua uuaagaagac     1320

aagaagauuu uuauccauuu uuaaaagaua auagagaaaa aauagaaaaa auauuaacau     1380

uuagaauacc auauuaugua ggaccauuag caagaggaaa uucaagauuu gcauggauga     1440

caagaaaauc agaagaaaca auaacaccau ggaauuuuga agaaguagua gauaaaggag     1500

caucagcaca aucauuuaua gaaagaauga caaauuuuga uaaaaauuua ccaaaugaaa     1560

aaguauuacc aaaacauuca uuauuauaug aauauuuuac aguauauaau gaauuaacaa     1620

aaguaaaaua uguaacagaa ggaaugagaa aaccagcauu uuuaucagga gaacaaaaaa     1680

aagcaauagu agauuuauua uuuaaaacaa auagaaaagu aacaguaaaa caauuaaaag     1740

aagauuauuu uaaaaaaaua gaauguuuug auucaguaga aauaucagga guagaagaua     1800

gauuuaaugc aucauuagga acauaucaug auuuauuaaa aauaauaaaa gauaaagauu     1860

uuuuagauaa ugaagaaaau gaagauauau uagaagauau aguauuaaca uuaacauuau     1920

uugaagauag agaaaugaua gaagaaagau uaaaaacaua ugcacauuua uuugaugaua     1980

aaguaaugaa acaauuaaaa agaagaagau auacaggaug gggaagauua ucaagaaaau     2040

uaauaaaugg aauaagagau aaacaaucag gaaaaacaau auuagauuuu uuaaaaucag     2100

auggauuugc aaauagaaau uuuaugcaau uaauacauga ugauucauua acauuuaaag     2160

aagauauaca aaaagcacaa guaucaggac aaggagauuc auuacaugaa cauauagcaa     2220

auuuagcagg aucaccagca auaaaaaaag gaauauuaca aacaguaaaa guaguagaug     2280

aauuaguaaa aguaauggga agacauaaac cagaaaauau aguaauagaa auggcaagag     2340

aaaaucaaac aacacaaaaa ggacaaaaaa auucaagaga aagaaugaaa agaauagaag     2400

aaggaauaaa agaauuagga ucacaaauau uaaaagaaca uccaguagaa aauacacaau     2460

uacaaaauga aaaauuauau uuauauuauu uacaaaaugg aagagauaug uauguagauc     2520

aagaauuaga uauaaauaga uuaucagauu augauguaga ucauauagua ccacaaucau     2580

uuuuaaaaga ugauucaaua gauaauaaag uauuaacaag aucagauaaa aauagaggaa     2640

aaucagauaa uguaccauca gaagaaguag uaaaaaaaau gaaaaauuau uggagacaau     2700

uauuaaaugc aaaauuaaua acacaaagaa aauuugauaa uuuaacaaaa gcagaaagag     2760

gaggauuauc agaauuagau aaagcaggau uuauaaaaag acaauuagua gaaacaagac     2820

aaauaacaaa acauguagca caaauauuag auucaagaau gaauacaaaa uaugaugaaa     2880

augauaaauu aauaagagaa guaaaaguaa uaacauuaaa aucaaaauua guaucagauu     2940

uuagaaaaga uuuucaauuu uauaaaguaa gagaaauaaa uaauuaucau caugcacaug     3000

augcauauuu aaaugcagua guaggaacag cauuaauaaa aaaauaucca aaauuagaau     3060

cagaauuugu auauggagau uauaaaguau augauguaag aaaaaugaua gcaaaaucag     3120

aacaagaaau aggaaaagca acagcaaaau auuuuuuuua uucaaauaua augaauuuuu     3180

uuaaaacaga aauaacauua gcaaauggag aaauaagaaa aagaccauua auagaaacaa     3240

auggagaaac aggagaaaua guaugggaua aaggaagaga uuuugcaaca guaagaaaag     3300

uauuaucaau gccacaagua aauauaguaa aaaaaacaga aguacaaaca ggaggauuuu     3360

caaaagaauc aauauuacca aaaagaaauu cagauaaauu aauagcaaga aaaaaagauu     3420

gggauccaaa aaaauaugga ggauuugauu caccaacagu agcauauuca guauuaguag     3480

uagcaaaagu agaaaaagga aaaucaaaaa aauuaaaauc aguaaaagaa uuauuaggaa     3540

uaacaauaau ggaaagauca ucauuugaaa aaaauccaau agauuuuuua gaagcaaaag     3600

gauauaaaga aguaaaaaaa gauuuaauaa uaaaauuacc aaaauauuca uuauuugaau     3660

uagaaaaugg aagaaaaaga auguuagcau cagcaggaga auuacaaaaa ggaaaugaau     3720

uagcauuacc aucaaaauau guaaauuuuu uauauuuagc aucacauuau gaaaaauuaa     3780

aaggaucacc agaagauaau gaacaaaaac aauuauuugu agaacaacau aaacauuauu     3840

uagaugaaau aauagaacaa auaucagaau uuucaaaaag aguaauauua gcagaugcaa     3900

auuuagauaa aguauuauca gcauauaaua aacauagaga uaaaccaaua agagaacaag     3960

cagaaaauau aauacauuua uuuacauuaa caaauuuagg agcaccagca gcauuuaaau     4020

auuuugauac aacaauagau agaaaaagau auacaucaac aaaagaagua uuagaugcaa     4080

cauuaauaca ucaaucaaua acaggauuau augaaacaag aauagauuua ucacaauuag     4140

gaggagaugg aggaggauca ccaaaaaaaa aaagaaaagu auagcuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucuc     4320

ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau cuag                      4424


<210>  24
<211>  4425
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 transcript comprising SEQ 14

<400>  24
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggau aaaaaguaca       60

gcaucggauu agauauagga acaaauucag uuggcugggc ugugauaaca gaugaauaua      120

aaguucccuc aaaaaaauuu aaaguauuag gaaauacaga uagacauagc aucaaaaaaa      180

aucucauagg ugcacuguua uuugauucag gugagacagc agaagccaca agauuaaaaa      240

gaacagcccg cagaagauau acaagaagaa aaaauagaau auguuauuua caggagauau      300

uuucaaauga aauggcaaaa guagaugauu cauuuuuuca uagauuagaa gaaucauucc      360

ugguagaaga agauaaaaaa caugaaagac auccaauauu uggaaauaua guagaugaag      420

ucgcauauca ugaaaaguac cccaccauau aucaucugcg gaaaaaauua guagauucga      480

cugauaaagc agaucugcgg uuaauauauu uagcacuggc acauaugaua aaauuuagag      540

gacauuuccu gauagaagga gauuuaaauc cugacaauuc agauguagau aaauuauuua      600

uacaauuagu acaaaccuac aaucaauuau uugaagaaaa uccaauaaau gcaucaggag      660

uagaugcaaa agcaauacuc agcgcccgcc ucagcaaauc aagaagauua gaaaaucuca      720

uagcacaacu uccaggugag aaaaaaaaug gguuauuugg aaaucucaua gcacucagcu      780

uaggauuaac ucccaauuuu aaaucaaauu uugauuuagc agaagaugca aaauuacaac      840

ucagcaaaga uaccuacgau gaugauuuag auaaucucuu agcacaaaua ggagaucaau      900

augcagauuu auuccuggcu gccaaaaauc ucagcgaugc aauauuacuc agcgauauac      960

ugcggguaaa uacagagaua acaaaagcac cacucagcgc aucaaugaua aaaagauaug     1020

augaacauca ucaagauuua acauuauuaa aagcacuggu aagacaacaa cuuccagaga     1080

aguacaaaga aauauuuuuu gaucagagca aaaaugggua ugccggguau auagauggug     1140

gugccucaca ggaggaauuu uauaaauuua uaaaaccaau auuagaaaaa auggauggaa     1200

cagaggagcu guuaguaaaa uuaaauaggg aggauuuacu gcggaaacaa agaacauuug     1260

auaaugggag caucccccau caaauacauu uaggugagcu gcaugcaaua cugcggagac     1320

aggaggauuu uuauccauuc cugaaagaua auagggagaa aauagaaaaa auauuaacau     1380

uuagaauccc cuauuauguu ggcccauuag cccgcggaaa uucaagauuu gcauggauga     1440

caagaaaauc agaagaaaca auaacucccu ggaauuuuga agaagucgua gauaagggug     1500

ccucagcaca gagcuuuaua gaaagaauga caaauuuuga uaaaaaucuu ccaaaugaaa     1560

aaguacuucc aaaacauuca uuauuauaug aauauuuuac aguauauaau gagcugacaa     1620

aaguaaagua cguaacagag ggaaugagaa aaccagcauu ccucagcggu gagcaaaaaa     1680

aagcaauagu agauuuauua uuuaaaacaa auagaaaagu aacaguaaaa caauuaaaag     1740

aagauuauuu uaaaaaaaua gaauguuuug auucaguaga aauaucagga guagaagaua     1800

gauuuaaugc aucauuagga accuaccaug auuuauuaaa aauaauaaaa gauaaagauu     1860

uccuggauaa ugaagaaaau gaagauauau uagaagauau aguauuaaca uuaacauuau     1920

uugaagauag ggagaugaua gaagaaagau uaaaaaccua cgcacauuua uuugaugaua     1980

aaguaaugaa acaauuaaaa agaagaagau auacaggaug gggaagacuc agcagaaaau     2040

uaauaaaugg gauacgagac aaacagagcg gaaaaacaau auuagauuuc cugaaaucag     2100

auggauuugc aaauagaaau uuuaugcaau uaauacauga ugauucauua acauuuaaag     2160

aagauauaca aaaagcacag gucagcggac agggcgauuc auuacaugaa cauauagcaa     2220

aucucgccgg gucaccagca auaaaaaagg ggauauuaca aacaguaaaa guaguagaug     2280

agcugguaaa aguaauggga agacauaaac cagagaauau aguaauagaa auggccaggg     2340

agaaucaaac aacucaaaag gggcaaaaaa auucaaggga gagaaugaaa agaauagaag     2400

aaggaauaaa agagcuggga ucacaaauau uaaaagaaca uccaguagaa aauacucaau     2460

uacaaaauga aaaauuauau uuauauuauu uacaaaaugg gcgagacaug uauguagauc     2520

aggagcugga uauaaauaga cucagcgauu augauguaga ucauauaguu ccccagagcu     2580

uccugaaaga ugauagcauc gauaauaaag uauuaacaag aucagauaaa aauagaggaa     2640

aaucagauaa uguucccuca gaagaagucg uaaaaaaaau gaaaaauuau uggagacaau     2700

uauuaaaugc aaaauuaaua acucaaagaa aauuugauaa ucucacaaaa gcagaaagag     2760

guggccucag cgagcuggau aaagccgggu uuauaaaaag acaauuagua gaaacaagac     2820

aaauaacaaa acauguagca caaauauuag auucaagaau gaauacaaag uacgaugaaa     2880

augauaaauu aauaagggaa gucaaaguaa uaacauuaaa aucaaaauua gucagcgauu     2940

uuagaaaaga uuuucaauuu uauaaaguaa gggagauaaa uaauuaucau caugcacaug     3000

augcauauuu aaaugcugug guuggcacag cacugauaaa aaaguaccca aaauuagaau     3060

cagaauuugu auauggagau uauaaaguau augauguaag aaaaaugaua gcaaaaucag     3120

aacaggagau aggaaaagca acagcaaagu acuuuuuuua uucaaauaua augaauuuuu     3180

uuaaaacaga gauaacauua gcaaauggug agauaagaaa aagaccauua auagaaacaa     3240

auggugagac aggugagaua guaugggaua aggggcgaga cuuugcaaca guaagaaaag     3300

uacucagcau gccacaggug aauauaguaa aaaaaacaga aguccaaaca gguggcuuuu     3360

caaaagaaag cauccuucca aaaagaaauu cagauaaauu aauagcccgc aaaaaagauu     3420

gggauccaaa aaaguacggu ggcuuugauu cacccaccgu agcauauuca guauuaguag     3480

uagcaaaagu agaaaagggg aaaucaaaaa aauuaaaauc aguaaaagag cuguuaggaa     3540

uaacaauaau ggaaagauca ucauuugaaa aaaauccaau agauuuccug gaagccaagg     3600

gguauaaaga agucaaaaaa gauuuaauaa uaaaacuucc aaaguacuca uuauuugagc     3660

uggaaaaugg gagaaaaaga auguuagcau cagccgguga gcugcaaaag gggaaugagc     3720

uggcacuucc cucaaaguac guaaauuucc uguauuuagc aucacauuau gaaaaauuaa     3780

aggggucacc agaggauaau gaacaaaaac aauuauuugu agaacaacau aaacauuauu     3840

uagaugaaau aauagaacaa auaucagaau uuucaaaaag aguaauauua gcagaugcaa     3900

aucucgauaa aguacucagc gcauauaaua aacaucgaga caaaccaaua agggagcagg     3960

ccgaaaauau aauacauuua uuuacauuaa caaaucucgg ugccccagcu gccuuuaagu     4020

acuuugauac aacaauagau agaaaaagau auacaucgac uaaagaaguc uuagaugcaa     4080

cauuaauaca ucagagcauc acaggauuau augaaacaag aauagaucuc agccaauuag     4140

guggcgaugg ugguggcuca ccaaaaaaaa aaagaaaagu auagcuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucuc     4320

ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ucuag                     4425


<210>  25

<400>  25
000

<210>  26

<400>  26
000

<210>  27

<400>  27
000

<210>  28

<400>  28
000

<210>  29
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: E-pair enriched, Table 6 codon enriched Cas9 ORF

<400>  29
auggacaaga aguacagcau cggacuggac aucggaacaa acagcguugg cugggcugug       60

aucacagacg aauacaaggu ucccucaaag aaguucaagg uccugggaaa cacagacaga      120

cacagcauca agaagaaucu caucggugca cugcuguucg acagcgguga gacagcagaa      180

gccacaagac ugaagagaac agcccgcaga agauacacaa gaagaaagaa cagaaucugc      240

uaccugcagg agaucuucag caacgaaaug gcaaaggucg acgacagcuu cuuccacaga      300

cuggaagaaa gcuuccuggu cgaagaagac aagaagcacg aaagacaccc gaucuucgga      360

aacaucgucg acgaagucgc auaccacgaa aaguacccca ccaucuacca ccugcggaag      420

aagcuggucg acucgacuga caaggcagac cugcggcuga ucuaccuggc acuggcacac      480

augauaaagu ucagaggaca cuuccugauc gaaggagacc ugaacccuga caacagcgac      540

gucgacaagc uguucaucca gcugguccag accuacaacc agcuguucga agaaaacccg      600

aucaacgcaa gcggagucga cgcaaaggca auccucagcg cccgccucag caagagcaga      660

agacuggaaa aucucaucgc acagcuucca ggugagaaga agaaugggcu guucggaaau      720

cucaucgcac ucagccuggg acugacuccc aacuucaaga gcaacuucga ccuggcagaa      780

gacgcaaagc ugcagcucag caaggacacc uacgacgacg accuggacaa ucuccuggca      840

cagaucggag accaguacgc agaccuguuc cuggcugcca agaaucucag cgacgcaauc      900

cugcucagcg acauccugcg ggucaacaca gagaucacaa aggcaccgcu cagcgcaagc      960

augauaaaga gauacgacga acaccaccag gaccugacac ugcugaaggc acuggucaga     1020

cagcagcuuc cagagaagua caaggaaauc uucuucgacc agagcaagaa uggguacgcc     1080

ggguacaucg acgguggugc cagccaggag gaauucuaca aguucaucaa gccgauccug     1140

gaaaagaugg acggaacaga ggagcugcug gucaagcuga acagggagga ccugcugcgg     1200

aagcagagaa cauucgacaa ugggagcauc ccccaccaga uccaccuggg ugagcugcac     1260

gcaauccugc ggagacagga ggacuucuac ccguuccuga aggacaacag ggagaagauc     1320

gaaaagaucc ugacauucag aauccccuac uacguuggcc cgcuggcccg cggaaacagc     1380

agauucgcau ggaugacaag aaagagcgaa gaaacaauca cucccuggaa cuucgaagaa     1440

gucgucgaca agggugccag cgcacagagc uucaucgaaa gaaugacaaa cuucgacaag     1500

aaucuuccaa acgaaaaggu ccuuccaaag cacagccugc uguacgaaua cuucacaguc     1560

uacaacgagc ugacaaaggu caaguacguc acagagggaa ugagaaagcc ggcauuccuc     1620

agcggugagc agaagaaggc aaucgucgac cugcuguuca agacaaacag aaaggucaca     1680

gucaagcagc ugaaggaaga cuacuucaag aagaucgaau gcuucgacag cgucgaaauc     1740

agcggagucg aagacagauu caacgcaagc cugggaaccu accacgaccu gcugaagauc     1800

aucaaggaca aggacuuccu ggacaacgaa gaaaacgaag acauccugga agacaucguc     1860

cugacacuga cacuguucga agacagggag augauagaag aaagacugaa gaccuacgca     1920

caccuguucg acgacaaggu caugaagcag cugaagagaa gaagauacac aggaugggga     1980

agacucagca gaaagcugau caaugggauc cgagacaagc agagcggaaa gacaauccug     2040

gacuuccuga agagcgacgg auucgcaaac agaaacuuca ugcagcugau ccacgacgac     2100

agccugacau ucaaggaaga cauccagaag gcacagguca gcggacaggg cgacagccug     2160

cacgaacaca ucgcaaaucu cgccgggagc ccggcaauca agaaggggau ccugcagaca     2220

gucaaggucg ucgacgagcu ggucaagguc augggaagac acaagccaga gaacaucguc     2280

aucgaaaugg ccagggagaa ccagacaacu caaaaggggc agaagaacag cagggagaga     2340

augaagagaa ucgaagaagg aaucaaggag cugggaagcc agauccugaa ggaacacccg     2400

gucgaaaaca cucaacugca gaacgaaaag cuguaccugu acuaccugca gaaugggcga     2460

gacauguacg ucgaccagga gcuggacauc aacagacuca gcgacuacga cgucgaccac     2520

aucguucccc agagcuuccu gaaggacgac agcaucgaca acaagguccu gacaagaagc     2580

gacaagaaca gaggaaagag cgacaacguu cccucagaag aagucgucaa gaagaugaag     2640

aacuacugga gacagcugcu gaacgcaaag cugaucacuc aaagaaaguu cgacaaucuc     2700

acaaaggcag aaagaggugg ccucagcgag cuggacaagg ccggguucau caagagacag     2760

cuggucgaaa caagacagau cacaaagcac gucgcacaga uccuggacag cagaaugaac     2820

acaaaguacg acgaaaacga caagcugauc agggaaguca aggucaucac acugaagagc     2880

aagcugguca gcgacuucag aaaggacuuc caguucuaca aggucaggga gaucaacaac     2940

uaccaccacg cacacgacgc auaccugaac gcugugguug gcacagcacu gaucaagaag     3000

uacccgaagc uggaaagcga auucgucuac ggagacuaca aggucuacga cgucagaaag     3060

augauagcaa agagcgaaca ggagaucgga aaggcaacag caaaguacuu cuucuacagc     3120

aacaucauga acuucuucaa gacagagauc acacuggcaa auggugagau cagaaagaga     3180

ccgcugaucg aaacaaaugg ugagacaggu gagaucgucu gggacaaggg gcgagacuuc     3240

gcaacaguca gaaagguccu cagcaugccg caggugaaca ucgucaagaa gacagaaguc     3300

cagacaggug gcuucagcaa ggaaagcauc cuuccaaaga gaaacagcga caagcugauc     3360

gcccgcaaga aggacuggga cccgaagaag uacgguggcu ucgacagccc caccgucgca     3420

uacagcgucc uggucgucgc aaaggucgaa aaggggaaga gcaagaagcu gaagagcguc     3480

aaggagcugc ugggaaucac aaucauggaa agaagcagcu ucgaaaagaa cccgaucgac     3540

uuccuggaag ccaaggggua caaggaaguc aagaaggacc ugaucaucaa gcuuccaaag     3600

uacagccugu ucgagcugga aaaugggaga aagagaaugc uggcaagcgc cggugagcug     3660

cagaagggga acgagcuggc acuucccuca aaguacguca acuuccugua ccuggcaagc     3720

cacuacgaaa agcugaaggg gagcccagag gacaacgaac agaagcagcu guucgucgaa     3780

cagcacaagc acuaccugga cgaaaucauc gaacagauca gcgaauucag caagagaguc     3840

auccuggcag acgcaaaucu cgacaagguc cucagcgcau acaacaagca ccgagacaag     3900

ccgaucaggg agcaggccga aaacaucauc caccuguuca cacugacaaa ucucggugcc     3960

ccggcugccu ucaaguacuu cgacacaaca aucgacagaa agagauacac aucgacuaag     4020

gaaguccugg acgcaacacu gauccaccag agcaucacag gacuguacga aacaagaauc     4080

gaccucagcc agcugggugg cgacgguggu ggcagcccga agaagaagag aaaggucuag     4140


<210>  30

<400>  30
000

<210>  31

<400>  31
000

<210>  32

<400>  32
000

<210>  33

<400>  33
000

<210>  34

<400>  34
000

<210>  35

<400>  35
000

<210>  36

<400>  36
000

<210>  37

<400>  37
000

<210>  38

<400>  38
000

<210>  39

<400>  39
000

<210>  40

<400>  40
000

<210>  41

<400>  41
000

<210>  42

<400>  42
000

<210>  43

<400>  43
000

<210>  44

<400>  44
000

<210>  45

<400>  45
000

<210>  46
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: E-pair enriched, Table 7 Low A codon enriched Cas9 ORF

<400>  46
auggacaaga aguacagcau cggccuggac aucggcacca acuccguugg cugggcugug       60

aucaccgacg aguacaaggu ucccucaaag aaguucaagg ugcugggcaa caccgaccgg      120

cacagcauca agaagaaucu caucggugca cugcuguucg acuccgguga gaccgccgaa      180

gccacccggc ugaagcggac cgcccgccgg cgguacaccc ggcggaagaa ccggaucugc      240

uaccugcagg agaucuucuc caacgagaug gccaaggugg acgacuccuu cuuccaccgg      300

cuggaggagu ccuuccuggu ggaggaggac aagaagcacg agcggcaccc caucuucggc      360

aacaucgugg acgaagucgc cuaccacgag aaguacccca ccaucuacca ccugcggaag      420

aagcuggugg acucgacuga caaggccgac cugcggcuga ucuaccuggc acuggcccac      480

augauaaagu uccggggcca cuuccugauc gagggcgacc ugaacccuga caacuccgac      540

guggacaagc uguucaucca gcuggugcag accuacaacc agcuguucga ggagaacccc      600

aucaacgccu ccggcgugga cgccaaggcc auccucagcg cccgccucag caagucccgg      660

cggcuggaga aucucaucgc ccagcuucca ggugagaaga agaaugggcu guucggcaau      720

cucaucgcac ucagccuggg ccugacuccc aacuucaagu ccaacuucga ccuggccgag      780

gacgccaagc ugcagcucag caaggacacc uacgacgacg accuggacaa ucuccuggcc      840

cagaucggcg accaguacgc cgaccuguuc cuggcugcca agaaucucag cgacgccauc      900

cugcucagcg acauccugcg ggugaacaca gagaucacca aggccccccu cagcgccucc      960

augauaaagc gguacgacga gcaccaccag gaccugaccc ugcugaaggc acuggugcgg     1020

cagcagcuuc cagagaagua caaggagauc uucuucgacc agagcaagaa uggguacgcc     1080

ggguacaucg acgguggugc cucccaggag gaguucuaca aguucaucaa gcccauccug     1140

gagaagaugg acggcacaga ggagcugcug gugaagcuga acagggagga ccugcugcgg     1200

aagcagcgga ccuucgacaa ugggagcauc ccccaccaga uccaccuggg ugagcugcac     1260

gccauccugc ggcggcagga ggacuucuac cccuuccuga aggacaacag ggagaagauc     1320

gagaagaucc ugaccuuccg gauccccuac uacguuggcc cccuggcccg cggcaacucc     1380

cgguucgccu ggaugacccg gaaguccgag gagaccauca cucccuggaa cuucgaggaa     1440

gucguggaca agggugccuc cgcccagagc uucaucgagc ggaugaccaa cuucgacaag     1500

aaucuuccaa acgagaaggu gcuuccaaag cacucccugc uguacgagua cuucaccgug     1560

uacaacgagc ugaccaaggu gaaguacgug acagagggca ugcggaagcc cgccuuccuc     1620

agcggugagc agaagaaggc caucguggac cugcuguuca agaccaaccg gaaggugacc     1680

gugaagcagc ugaaggagga cuacuucaag aagaucgagu gcuucgacuc cguggagauc     1740

uccggcgugg aggaccgguu caacgccucc cugggcaccu accacgaccu gcugaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccugga ggacaucgug     1860

cugacccuga cccuguucga ggacagggag augauagagg agcggcugaa gaccuacgcc     1920

caccuguucg acgacaaggu gaugaagcag cugaagcggc ggcgguacac cggcuggggc     1980

cggcucagcc ggaagcugau caaugggauc cgagacaagc agagcggcaa gaccauccug     2040

gacuuccuga aguccgacgg cuucgccaac cggaacuuca ugcagcugau ccacgacgac     2100

ucccugaccu ucaaggagga cauccagaag gcccagguca gcggccaggg cgacucccug     2160

cacgagcaca ucgccaaucu cgccgggucc cccgccauca agaaggggau ccugcagacc     2220

gugaaggugg uggacgagcu ggugaaggug augggccggc acaagccaga gaacaucgug     2280

aucgagaugg ccagggagaa ccagaccacu caaaaggggc agaagaacuc cagggagcgg     2340

augaagcgga ucgaggaggg caucaaggag cugggcuccc agauccugaa ggagcacccc     2400

guggagaaca cucaacugca gaacgagaag cuguaccugu acuaccugca gaaugggcga     2460

gacauguacg uggaccagga gcuggacauc aaccggcuca gcgacuacga cguggaccac     2520

aucguucccc agagcuuccu gaaggacgac agcaucgaca acaaggugcu gacccggucc     2580

gacaagaacc ggggcaaguc cgacaacguu cccucagagg aagucgugaa gaagaugaag     2640

aacuacuggc ggcagcugcu gaacgccaag cugaucacuc aacggaaguu cgacaaucuc     2700

accaaggccg agcggggugg ccucagcgag cuggacaagg ccggguucau caagcggcag     2760

cugguggaga cccggcagau caccaagcac guggcccaga uccuggacuc ccggaugaac     2820

accaaguacg acgagaacga caagcugauc agggaaguca aggugaucac ccugaagucc     2880

aagcugguca gcgacuuccg gaaggacuuc caguucuaca aggugaggga gaucaacaac     2940

uaccaccacg cccacgacgc cuaccugaac gcugugguug gcaccgcacu gaucaagaag     3000

uaccccaagc uggaguccga guucguguac ggcgacuaca agguguacga cgugcggaag     3060

augauagcca aguccgagca ggagaucggc aaggccaccg ccaaguacuu cuucuacucc     3120

aacaucauga acuucuucaa gacagagauc acccuggcca auggugagau ccggaagcgg     3180

ccccugaucg agaccaaugg ugagaccggu gagaucgugu gggacaaggg gcgagacuuc     3240

gccaccgugc ggaaggugcu cagcaugccc caggugaaca ucgugaagaa gacagaaguc     3300

cagaccggug gcuucuccaa ggagagcauc cuuccaaagc ggaacuccga caagcugauc     3360

gcccgcaaga aggacuggga ccccaagaag uacgguggcu ucgacucccc caccguggcc     3420

uacuccgugc uggugguggc caagguggag aaggggaagu ccaagaagcu gaaguccgug     3480

aaggagcugc ugggcaucac caucauggag cgguccuccu ucgagaagaa ccccaucgac     3540

uuccuggaag ccaaggggua caaggaaguc aagaaggacc ugaucaucaa gcuuccaaag     3600

uacucccugu ucgagcugga gaaugggcgg aagcggaugc uggccuccgc cggugagcug     3660

cagaagggga acgagcuggc acuucccuca aaguacguga acuuccugua ccuggccucc     3720

cacuacgaga agcugaaggg guccccagag gacaacgagc agaagcagcu guucguggag     3780

cagcacaagc acuaccugga cgagaucauc gagcagaucu ccgaguucuc caagcgggug     3840

auccuggccg acgccaaucu cgacaaggug cucagcgccu acaacaagca ccgagacaag     3900

cccaucaggg agcaggccga gaacaucauc caccuguuca cccugaccaa ucucggugcc     3960

cccgcugccu ucaaguacuu cgacaccacc aucgaccgga agcgguacac cucgacuaag     4020

gaaguccugg acgccacccu gauccaccag agcaucaccg gccuguacga gacccggauc     4080

gaccucagcc agcugggugg cgacgguggu ggcuccccca agaagaagcg gaagguguag     4140


<210>  47

<400>  47
000

<210>  48

<400>  48
000

<210>  49

<400>  49
000

<210>  50

<400>  50
000

<210>  51

<400>  51
000

<210>  52

<400>  52
000

<210>  53

<400>  53
000

<210>  54

<400>  54
000

<210>  55

<400>  55
000

<210>  56

<400>  56
000

<210>  57

<400>  57
000

<210>  58

<400>  58
000

<210>  59

<400>  59
000

<210>  60

<400>  60
000

<210>  61

<400>  61
000

<210>  62

<400>  62
000

<210>  63

<400>  63
000

<210>  64

<400>  64
000

<210>  65

<400>  65
000

<210>  66

<400>  66
000

<210>  67
<211>  4104
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: WT Cas9 ORF

<400>  67
atggataaga agtactcaat cgggctggat atcggaacta attccgtggg ttgggcagtg       60

atcacggatg aatacaaagt gccgtccaag aagttcaagg tcctggggaa caccgataga      120

cacagcatca agaaaaatct catcggagcc ctgctgtttg actccggcga aaccgcagaa      180

gcgacccggc tcaaacgtac cgcgaggcga cgctacaccc ggcggaagaa tcgcatctgc      240

tatctgcaag agatcttttc gaacgaaatg gcaaaggtcg acgacagctt cttccaccgc      300

ctggaagaat ctttcctggt ggaggaggac aagaagcatg aacggcatcc tatctttgga      360

aacatcgtcg acgaagtggc gtaccacgaa aagtacccga ccatctacca tctgcggaag      420

aagttggttg actcaactga caaggccgac ctcagattga tctacttggc cctcgcccat      480

atgatcaaat tccgcggaca cttcctgatc gaaggcgatc tgaaccctga taactccgac      540

gtggataagc ttttcattca actggtgcag acctacaacc aactgttcga agaaaaccca      600

atcaatgcta gcggcgtcga tgccaaggcc atcctgtccg cccggctgtc gaagtcgcgg      660

cgcctcgaaa acctgatcgc acagctgccg ggagagaaaa agaacggact tttcggcaac      720

ttgatcgctc tctcactggg actcactccc aatttcaagt ccaattttga cctggccgag      780

gacgcgaagc tgcaactctc aaaggacacc tacgacgacg acttggacaa tttgctggca      840

caaattggcg atcagtacgc ggatctgttc cttgccgcta agaacctttc ggacgcaatc      900

ttgctgtccg atatcctgcg cgtgaacacc gaaataacca aagcgccgct tagcgcctcg      960

atgattaagc ggtacgacga gcatcaccag gatctcacgc tgctcaaagc gctcgtgaga     1020

cagcaactgc ctgaaaagta caaggagatc ttcttcgacc agtccaagaa tgggtacgca     1080

gggtacatcg atggaggcgc tagccaggaa gagttctata agttcatcaa gccaatcctg     1140

gaaaagatgg acggaaccga agaactgctg gtcaagctga acagggagga tctgctccgg     1200

aaacagagaa cctttgacaa cggatccatt ccccaccaga tccatctggg tgagctgcac     1260

gccatcttgc ggcgccagga ggacttttac ccattcctca aggacaaccg ggaaaagatc     1320

gagaaaattc tgacgttccg catcccgtat tacgtgggcc cactggcgcg cggcaattcg     1380

cgcttcgcgt ggatgactag aaaatcagag gaaaccatca ctccttggaa tttcgaggaa     1440

gttgtggata agggagcttc ggcacaaagc ttcatcgaac gaatgaccaa cttcgacaag     1500

aatctcccaa acgagaaggt gcttcctaag cacagcctcc tttacgaata cttcactgtc     1560

tacaacgaac tgactaaagt gaaatacgtt actgaaggaa tgaggaagcc ggcctttctg     1620

tccggagaac agaagaaagc aattgtcgat ctgctgttca agaccaaccg caaggtgacc     1680

gtcaagcagc ttaaagagga ctacttcaag aagatcgagt gtttcgactc agtggaaatc     1740

agcggggtgg aggacagatt caacgcttcg ctgggaacct atcatgatct cctgaagatc     1800

atcaaggaca aggacttcct tgacaacgag gagaacgagg acatcctgga agatatcgtc     1860

ctgaccttga cccttttcga ggatcgcgag atgatcgagg agaggcttaa gacctacgct     1920

catctcttcg acgataaggt catgaaacaa ctcaagcgcc gccggtacac tggttggggc     1980

cgcctctccc gcaagctgat caacggtatt cgcgataaac agagcggtaa aactatcctg     2040

gatttcctca aatcggatgg cttcgctaat cgtaacttca tgcaattgat ccacgacgac     2100

agcctgacct ttaaggagga catccaaaaa gcacaagtgt ccggacaggg agactcactc     2160

catgaacaca tcgcgaatct ggccggttcg ccggcgatta agaagggaat tctgcaaact     2220

gtgaaggtgg tcgacgagct ggtgaaggtc atgggacggc acaaaccgga gaatatcgtg     2280

attgaaatgg cccgagaaaa ccagactacc cagaagggcc agaaaaactc ccgcgaaagg     2340

atgaagcgga tcgaagaagg aatcaaggag ctgggcagcc agatcctgaa agagcacccg     2400

gtggaaaaca cgcagctgca gaacgagaag ctctacctgt actatttgca aaatggacgg     2460

gacatgtacg tggaccaaga gctggacatc aatcggttgt ctgattacga cgtggaccac     2520

atcgttccac agtcctttct gaaggatgac tcgatcgata acaaggtgtt gactcgcagc     2580

gacaagaaca gagggaagtc agataatgtg ccatcggagg aggtcgtgaa gaagatgaag     2640

aattactggc ggcagctcct gaatgcgaag ctgattaccc agagaaagtt tgacaatctc     2700

actaaagccg agcgcggcgg actctcagag ctggataagg ctggattcat caaacggcag     2760

ctggtcgaga ctcggcagat taccaagcac gtggcgcaga tcttggactc ccgcatgaac     2820

actaaatacg acgagaacga taagctcatc cgggaagtga aggtgattac cctgaaaagc     2880

aaacttgtgt cggactttcg gaaggacttt cagttttaca aagtgagaga aatcaacaac     2940

taccatcacg cgcatgacgc atacctcaac gctgtggtcg gtaccgccct gatcaaaaag     3000

taccctaaac ttgaatcgga gtttgtgtac ggagactaca aggtctacga cgtgaggaag     3060

atgatagcca agtccgaaca ggaaatcggg aaagcaactg cgaaatactt cttttactca     3120

aacatcatga actttttcaa gactgaaatt acgctggcca atggagaaat caggaagagg     3180

ccactgatcg aaactaacgg agaaacgggc gaaatcgtgt gggacaaggg cagggacttc     3240

gcaactgttc gcaaagtgct ctctatgccg caagtcaata ttgtgaagaa aaccgaagtg     3300

caaaccggcg gattttcaaa ggaatcgatc ctcccaaaga gaaatagcga caagctcatt     3360

gcacgcaaga aagactggga cccgaagaag tacggaggat tcgattcgcc gactgtcgca     3420

tactccgtcc tcgtggtggc caaggtggag aagggaaaga gcaaaaagct caaatccgtc     3480

aaagagctgc tggggattac catcatggaa cgatcctcgt tcgagaagaa cccgattgat     3540

ttcctcgagg cgaagggtta caaggaggtg aagaaggatc tgatcatcaa actccccaag     3600

tactcactgt tcgaactgga aaatggtcgg aagcgcatgc tggcttcggc cggagaactc     3660

caaaaaggaa atgagctggc cttgcctagc aagtacgtca acttcctcta tcttgcttcg     3720

cactacgaaa aactcaaagg gtcaccggaa gataacgaac agaagcagct tttcgtggag     3780

cagcacaagc attatctgga tgaaatcatc gaacaaatct ccgagttttc aaagcgcgtg     3840

atcctcgccg acgccaacct cgacaaagtc ctgtcggcct acaataagca tagagataag     3900

ccgatcagag aacaggccga gaacattatc cacttgttca ccctgactaa cctgggagcc     3960

ccagccgcct tcaagtactt cgatactact atcgatcgca aaagatacac gtccaccaag     4020

gaagttctgg acgcgaccct gatccaccaa agcatcactg gactctacga aactaggatc     4080

gatctgtcgc agctgggtgg cgat                                            4104


<210>  68
<211>  1257
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: WT SERPINA1 ORF

<400>  68
atgccgtctt ctgtctcgtg gggcatcctc ctgctggcag gcctgtgctg cctggtccct       60

gtctccctgg ctgaggatcc ccagggagat gctgcccaga agacagatac atcccaccat      120

gatcaggatc acccaacctt caacaagatc acccccaacc tggctgagtt cgccttcagc      180

ctataccgcc agctggcaca ccagtccaac agcaccaata tcttcttctc cccagtgagc      240

atcgctacag cctttgcaat gctctccctg gggaccaagg ctgacactca cgatgaaatc      300

ctggagggcc tgaatttcaa cctcacggag attccggagg ctcagatcca tgaaggcttc      360

caggaactcc tccgtaccct caaccagcca gacagccagc tccagctgac caccggcaat      420

ggcctgttcc tcagcgaggg cctgaagcta gtggataagt ttttggagga tgttaaaaag      480

ttgtaccact cagaagcctt cactgtcaac ttcggggaca ccgaagaggc caagaaacag      540

atcaacgatt acgtggagaa gggtactcaa gggaaaattg tggatttggt caaggagctt      600

gacagagaca cagtttttgc tctggtgaat tacatcttct ttaaaggcaa atgggagaga      660

ccctttgaag tcaaggacac cgaggaagag gacttccacg tggaccaggt gaccaccgtg      720

aaggtgccta tgatgaagcg tttaggcatg tttaacatcc agcactgtaa gaagctgtcc      780

agctgggtgc tgctgatgaa atacctgggc aatgccaccg ccatcttctt cctgcctgat      840

gaggggaaac tacagcacct ggaaaatgaa ctcacccacg atatcatcac caagttcctg      900

gaaaatgaag acagaaggtc tgccagctta catttaccca aactgtccat tactggaacc      960

tatgatctga agagcgtcct gggtcaactg ggcatcacta aggtcttcag caatggggct     1020

gacctctccg gggtcacaga ggaggcaccc ctgaagctct ccaaggccgt gcataaggct     1080

gtgctgacca tcgacgagaa agggactgaa gctgctgggg ccatgttttt agaggccata     1140

cccatgtcta tcccccccga ggtcaagttc aacaaaccct ttgtcttctt aatgattgaa     1200

caaaatacca agtctcccct cttcatggga aaagtggtga atcccaccca aaaataa        1257


<210>  69
<211>  1257
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SERPINA1 ORF using codons of Table 5

<400>  69
atgccatctt ctgtctcttg gggtatcttg ttgttggccg gtttgtgctg cttggtccca       60

gtctctttgg ccgaagaccc acaaggtgac gccgcccaaa agaccgacac ctctcaccac      120

gaccaagacc acccaacctt caacaagatc accccaaact tggccgaatt cgccttctct      180

ttgtacagac aattggccca ccaatctaac tctaccaaca tcttcttctc tccagtctct      240

atcgccaccg ccttcgccat gttgtctttg ggtaccaagg ccgacaccca cgacgaaatc      300

ttggaaggtt tgaacttcaa cttgaccgaa atcccagaag cccaaatcca cgaaggtttc      360

caagaattgt tgagaacctt gaaccaacca gactctcaat tgcaattgac caccggtaac      420

ggtttgttct tgtctgaagg tttgaagttg gtcgacaagt tcttggaaga cgtcaagaag      480

ttgtaccact ctgaagcctt caccgtcaac ttcggtgaca ccgaagaagc caagaagcaa      540

atcaacgact acgtcgaaaa gggtacccaa ggtaagatcg tcgacttggt caaggaattg      600

gacagagaca ccgtcttcgc cttggtcaac tacatcttct tcaagggtaa gtgggaaaga      660

ccattcgaag tcaaggacac cgaagaagaa gacttccacg tcgaccaagt caccaccgtc      720

aaggtcccaa tgatgaagag attgggtatg ttcaacatcc aacactgcaa gaagttgtct      780

tcttgggtct tgttgatgaa gtacttgggt aacgccaccg ccatcttctt cttgccagac      840

gaaggtaagt tgcaacactt ggaaaacgaa ttgacccacg acatcatcac caagttcttg      900

gaaaacgaag acagaagatc tgcctctttg cacttgccaa agttgtctat caccggtacc      960

tacgacttga agtctgtctt gggtcaattg ggtatcacca aggtcttctc taacggtgcc     1020

gacttgtctg gtgtcaccga agaagcccca ttgaagttgt ctaaggccgt ccacaaggcc     1080

gtcttgacca tcgacgaaaa gggtaccgaa gccgccggtg ccatgttctt ggaagccatc     1140

ccaatgtcta tcccaccaga agtcaagttc aacaagccat tcgtcttctt gatgatcgaa     1200

caaaacacca agtctccatt gttcatgggt aaggtcgtca acccaaccca aaagtaa        1257


<210>  70
<211>  1257
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SERPINA1 ORF using codons of Table 6

<400>  70
atgccgtcgt cggtctcgtg gggaatcctg ctgctggcag gactgtgctg cctggtcccg       60

gtctcgctgg cagaagaccc gcagggagac gcagcacaga agacagacac atcgcaccac      120

gaccaggacc acccgacatt caacaagatc acaccgaacc tggcagaatt cgcattctcg      180

ctgtacagac agctggcaca ccagtcgaac tcgacaaaca tcttcttctc gccggtctcg      240

atcgcaacag cattcgcaat gctgtcgctg ggaacaaagg cagacacaca cgacgaaatc      300

ctggaaggac tgaacttcaa cctgacagaa atcccggaag cacagatcca cgaaggattc      360

caggaactgc tgagaacact gaaccagccg gactcgcagc tgcagctgac aacaggaaac      420

ggactgttcc tgtcggaagg actgaagctg gtcgacaagt tcctggaaga cgtcaagaag      480

ctgtaccact cggaagcatt cacagtcaac ttcggagaca cagaagaagc aaagaagcag      540

atcaacgact acgtcgaaaa gggaacacag ggaaagatcg tcgacctggt caaggaactg      600

gacagagaca cagtcttcgc actggtcaac tacatcttct tcaagggaaa gtgggaaaga      660

ccgttcgaag tcaaggacac agaagaagaa gacttccacg tcgaccaggt cacaacagtc      720

aaggtcccga tgatgaagag actgggaatg ttcaacatcc agcactgcaa gaagctgtcg      780

tcgtgggtcc tgctgatgaa gtacctggga aacgcaacag caatcttctt cctgccggac      840

gaaggaaagc tgcagcacct ggaaaacgaa ctgacacacg acatcatcac aaagttcctg      900

gaaaacgaag acagaagatc ggcatcgctg cacctgccga agctgtcgat cacaggaaca      960

tacgacctga agtcggtcct gggacagctg ggaatcacaa aggtcttctc gaacggagca     1020

gacctgtcgg gagtcacaga agaagcaccg ctgaagctgt cgaaggcagt ccacaaggca     1080

gtcctgacaa tcgacgaaaa gggaacagaa gcagcaggag caatgttcct ggaagcaatc     1140

ccgatgtcga tcccgccgga agtcaagttc aacaagccgt tcgtcttcct gatgatcgaa     1200

cagaacacaa agtcgccgct gttcatggga aaggtcgtca acccgacaca gaagtga        1257


<210>  71
<211>  1257
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SERPINA1 ORF 1.1

<400>  71
atgcccagca gcgtgagctg gggcatcctg ctgctggccg gcctgtgctg cctggtgccc       60

gtgagcctgg ccgaggaccc ccagggcgac gccgcccaga agacggacac gagccaccac      120

gaccaggacc accccacgtt caacaagatc acgcccaacc tggccgagtt cgccttcagc      180

ctgtaccggc agctggccca ccagagcaac agcacgaaca tcttcttcag ccccgtgagc      240

atcgccacgg ccttcgccat gctgagcctg ggcacgaagg ccgacacgca cgacgagatc      300

ctggagggcc tgaacttcaa cctgacggag atccccgagg cccagatcca cgagggcttc      360

caggagctgc tgcggacgct gaaccagccc gacagccagc tgcagctgac gacgggcaac      420

ggcctgttcc tgagcgaggg cctgaagctg gtggacaagt tcctggagga cgtgaagaag      480

ctgtaccaca gcgaggcctt cacggtgaac ttcggcgaca cggaggaggc caagaagcag      540

atcaacgact acgtggagaa gggcacgcag ggcaagatcg tggacctggt gaaggagctg      600

gaccgggaca cggtgttcgc cctggtgaac tacatcttct tcaagggcaa gtgggagcgg      660

cccttcgagg tgaaggacac ggaggaggag gacttccacg tggaccaggt gacgacggtg      720

aaggtgccca tgatgaagcg gctgggcatg ttcaacatcc agcactgcaa gaagctgagc      780

agctgggtgc tgctgatgaa gtacctgggc aacgccacgg ccatcttctt cctgcccgac      840

gagggcaagc tgcagcacct ggagaacgag ctgacgcacg acatcatcac gaagttcctg      900

gagaacgagg accggcggag cgccagcctg cacctgccca agctgagcat cacgggcacg      960

tacgacctga agagcgtgct gggccagctg ggcatcacga aggtgttcag caacggcgcc     1020

gacctgagcg gcgtgacgga ggaggccccc ctgaagctga gcaaggccgt gcacaaggcc     1080

gtgctgacga tcgacgagaa gggcacggag gccgccggcg ccatgttcct ggaggccatc     1140

cccatgagca tcccccccga ggtgaagttc aacaagccct tcgtgttcct gatgatcgag     1200

cagaacacga agagccccct gttcatgggc aaggtggtga accccacgca gaagtag        1257


<210>  72
<211>  1257
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SERPINA1 ORF 1.2

<400>  72
atgccctcgt cggtctcgtg gggcatcctc ctcctcgcgg gcctctgctg cctcgtcccc       60

gtctcgctcg cggaggaccc ccagggcgac gcggcgcaga agacggacac gtcgcaccac      120

gaccaggacc accccacgtt caacaagatc acgcccaacc tcgcggagtt cgcgttctcg      180

ctctaccgcc agctcgcgca ccagtcgaac tcgacgaaca tcttcttctc gcccgtctcg      240

atcgcgacgg cgttcgcgat gctctcgctc ggcacgaagg cggacacgca cgacgagatc      300

ctcgagggcc tcaacttcaa cctcacggag atccccgagg cgcagatcca cgagggcttc      360

caggagctcc tccgcacgct caaccagccc gactcgcagc tccagctcac gacgggcaac      420

ggcctcttcc tctcggaggg cctcaagctc gtcgacaagt tcctcgagga cgtcaagaag      480

ctctaccact cggaggcgtt cacggtcaac ttcggcgaca cggaggaggc gaagaagcag      540

atcaacgact acgtcgagaa gggcacgcag ggcaagatcg tcgacctcgt caaggagctc      600

gaccgcgaca cggtcttcgc gctcgtcaac tacatcttct tcaagggcaa gtgggagcgc      660

cccttcgagg tcaaggacac ggaggaggag gacttccacg tcgaccaggt cacgacggtc      720

aaggtcccca tgatgaagcg cctcggcatg ttcaacatcc agcactgcaa gaagctctcg      780

tcgtgggtcc tcctcatgaa gtacctcggc aacgcgacgg cgatcttctt cctccccgac      840

gagggcaagc tccagcacct cgagaacgag ctcacgcacg acatcatcac gaagttcctc      900

gagaacgagg accgccgctc ggcgtcgctc cacctcccca agctctcgat cacgggcacg      960

tacgacctca agtcggtcct cggccagctc ggcatcacga aggtcttctc gaacggcgcg     1020

gacctctcgg gcgtcacgga ggaggcgccc ctcaagctct cgaaggcggt ccacaaggcg     1080

gtcctcacga tcgacgagaa gggcacggag gcggcgggcg cgatgttcct cgaggcgatc     1140

cccatgtcga tcccccccga ggtcaagttc aacaagccct tcgtcttcct catgatcgag     1200

cagaacacga agtcgcccct cttcatgggc aaggtcgtca accccacgca gaagtag        1257


<210>  73
<211>  1257
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SERPINA1 ORF 1.3

<400>  73
atgccctcat cggtcagctg gggcatcctc ctcctcgccg ggctctgctg cctcgttccc       60

gtcagcctcg cggaggaccc ccagggcgac gctgcccaga agacggacac gtcgcaccac      120

gaccaggacc accccacctt caacaagatc actcccaatc tcgcggagtt cgcgttctcg      180

ctctaccgcc agctcgcgca ccagagcaac tcgactaaca tcttcttctc gcccgtcagc      240

atcgcgacgg cgttcgcgat gctcagcctc ggcacgaagg cggacacgca cgacgagatc      300

ctcgagggcc tcaacttcaa tctcacagag atcccagaag cccagatcca cgagggcttc      360

caggagctgc tgcggacgct caaccagcct gactcgcagc tccagctcac gacgggcaat      420

gggctcttcc tcagcgaggg cctcaagctc gtcgacaagt tcctggagga cgtcaagaag      480

ctctaccact cggaagcctt cacggtcaac ttcggcgaca cagaggaagc caagaagcag      540

atcaacgact acgtcgagaa ggggactcag ggcaagatcg tcgacctcgt caaggagctg      600

gaccgagaca cggtcttcgc actggtcaac tacatcttct tcaaggggaa gtgggagcgc      660

cccttcgaag tcaaggacac agaggaggag gacttccacg tcgaccaggt gacgacggtc      720

aaggttccca tgatgaagcg cctcggcatg ttcaacatcc agcactgcaa gaagctcagc      780

tcgtgggtcc tcctcatgaa gtacctcggc aacgcgacgg cgatcttctt ccttcctgac      840

gagggcaagc tccagcacct cgagaacgag ctgacgcacg acatcatcac gaagttcctg      900

gagaacgagg accgccgatc ggcgtcgctc caccttccaa agctcagcat cacgggcacc      960

tacgacctca agtcggtcct cggccagctc ggcatcacga aggtcttctc gaatggtgcc     1020

gacctcagcg gcgtcacaga ggaagccccc ctcaagctca gcaaggctgt gcacaaggct     1080

gtgctcacga tcgacgagaa ggggacagaa gctgccggtg ccatgttcct ggaagccatc     1140

cccatgagca tcccaccaga agtcaagttc aacaagccct tcgtcttcct gatgatagag     1200

cagaacacga agtcgcccct cttcatgggc aaggtcgtca accccactca aaagtag        1257


<210>  74
<211>  418
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: SERPINA1 WT amino acid sequence

<400>  74

Met Pro Ser Ser Val Ser Trp Gly Ile Leu Leu Leu Ala Gly Leu Cys 
1               5                   10                  15      


Cys Leu Val Pro Val Ser Leu Ala Glu Asp Pro Gln Gly Asp Ala Ala 
            20                  25                  30          


Gln Lys Thr Asp Thr Ser His His Asp Gln Asp His Pro Thr Phe Asn 
        35                  40                  45              


Lys Ile Thr Pro Asn Leu Ala Glu Phe Ala Phe Ser Leu Tyr Arg Gln 
    50                  55                  60                  


Leu Ala His Gln Ser Asn Ser Thr Asn Ile Phe Phe Ser Pro Val Ser 
65                  70                  75                  80  


Ile Ala Thr Ala Phe Ala Met Leu Ser Leu Gly Thr Lys Ala Asp Thr 
                85                  90                  95      


His Asp Glu Ile Leu Glu Gly Leu Asn Phe Asn Leu Thr Glu Ile Pro 
            100                 105                 110         


Glu Ala Gln Ile His Glu Gly Phe Gln Glu Leu Leu Arg Thr Leu Asn 
        115                 120                 125             


Gln Pro Asp Ser Gln Leu Gln Leu Thr Thr Gly Asn Gly Leu Phe Leu 
    130                 135                 140                 


Ser Glu Gly Leu Lys Leu Val Asp Lys Phe Leu Glu Asp Val Lys Lys 
145                 150                 155                 160 


Leu Tyr His Ser Glu Ala Phe Thr Val Asn Phe Gly Asp Thr Glu Glu 
                165                 170                 175     


Ala Lys Lys Gln Ile Asn Asp Tyr Val Glu Lys Gly Thr Gln Gly Lys 
            180                 185                 190         


Ile Val Asp Leu Val Lys Glu Leu Asp Arg Asp Thr Val Phe Ala Leu 
        195                 200                 205             


Val Asn Tyr Ile Phe Phe Lys Gly Lys Trp Glu Arg Pro Phe Glu Val 
    210                 215                 220                 


Lys Asp Thr Glu Glu Glu Asp Phe His Val Asp Gln Val Thr Thr Val 
225                 230                 235                 240 


Lys Val Pro Met Met Lys Arg Leu Gly Met Phe Asn Ile Gln His Cys 
                245                 250                 255     


Lys Lys Leu Ser Ser Trp Val Leu Leu Met Lys Tyr Leu Gly Asn Ala 
            260                 265                 270         


Thr Ala Ile Phe Phe Leu Pro Asp Glu Gly Lys Leu Gln His Leu Glu 
        275                 280                 285             


Asn Glu Leu Thr His Asp Ile Ile Thr Lys Phe Leu Glu Asn Glu Asp 
    290                 295                 300                 


Arg Arg Ser Ala Ser Leu His Leu Pro Lys Leu Ser Ile Thr Gly Thr 
305                 310                 315                 320 


Tyr Asp Leu Lys Ser Val Leu Gly Gln Leu Gly Ile Thr Lys Val Phe 
                325                 330                 335     


Ser Asn Gly Ala Asp Leu Ser Gly Val Thr Glu Glu Ala Pro Leu Lys 
            340                 345                 350         


Leu Ser Lys Ala Val His Lys Ala Val Leu Thr Ile Asp Glu Lys Gly 
        355                 360                 365             


Thr Glu Ala Ala Gly Ala Met Phe Leu Glu Ala Ile Pro Met Ser Ile 
    370                 375                 380                 


Pro Pro Glu Val Lys Phe Asn Lys Pro Phe Val Phe Leu Met Ile Glu 
385                 390                 395                 400 


Gln Asn Thr Lys Ser Pro Leu Phe Met Gly Lys Val Val Asn Pro Thr 
                405                 410                 415     


Gln Lys 
        


<210>  75

<400>  75
000

<210>  76
<211>  1622
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SERPINA1 transcript comprising SEQ 70

<400>  76
gggtcccgca gtcggcgtcc agcggctctg cttgttcgtg tgtgtgtcgt tgcaggcctt       60

attcggatca gccaccatgc cgtcgtcggt ctcgtgggga atcctgctgc tggcaggact      120

gtgctgcctg gtcccggtct cgctggcaga agacccgcag ggagacgcag cacagaagac      180

agacacatcg caccacgacc aggaccaccc gacattcaac aagatcacac cgaacctggc      240

agaattcgca ttctcgctgt acagacagct ggcacaccag tcgaactcga caaacatctt      300

cttctcgccg gtctcgatcg caacagcatt cgcaatgctg tcgctgggaa caaaggcaga      360

cacacacgac gaaatcctgg aaggactgaa cttcaacctg acagaaatcc cggaagcaca      420

gatccacgaa ggattccagg aactgctgag aacactgaac cagccggact cgcagctgca      480

gctgacaaca ggaaacggac tgttcctgtc ggaaggactg aagctggtcg acaagttcct      540

ggaagacgtc aagaagctgt accactcgga agcattcaca gtcaacttcg gagacacaga      600

agaagcaaag aagcagatca acgactacgt cgaaaaggga acacagggaa agatcgtcga      660

cctggtcaag gaactggaca gagacacagt cttcgcactg gtcaactaca tcttcttcaa      720

gggaaagtgg gaaagaccgt tcgaagtcaa ggacacagaa gaagaagact tccacgtcga      780

ccaggtcaca acagtcaagg tcccgatgat gaagagactg ggaatgttca acatccagca      840

ctgcaagaag ctgtcgtcgt gggtcctgct gatgaagtac ctgggaaacg caacagcaat      900

cttcttcctg ccggacgaag gaaagctgca gcacctggaa aacgaactga cacacgacat      960

catcacaaag ttcctggaaa acgaagacag aagatcggca tcgctgcacc tgccgaagct     1020

gtcgatcaca ggaacatacg acctgaagtc ggtcctggga cagctgggaa tcacaaaggt     1080

cttctcgaac ggagcagacc tgtcgggagt cacagaagaa gcaccgctga agctgtcgaa     1140

ggcagtccac aaggcagtcc tgacaatcga cgaaaaggga acagaagcag caggagcaat     1200

gttcctggaa gcaatcccga tgtcgatccc gccggaagtc aagttcaaca agccgttcgt     1260

cttcctgatg atcgaacaga acacaaagtc gccgctgttc atgggaaagg tcgtcaaccc     1320

gacacagaag tagctagcca tcacatttaa aagcatctca gcctaccatg agaataagag     1380

aaagaaaatg aagatcaata gcttattcat ctctttttct ttttcgttgg tgtaaagcca     1440

acaccctgtc taaaaaacat aaatttcttt aatcattttg cctcttttct ctgtgcttca     1500

attaataaaa aatggaaaga acctcgagaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaatct     1620

ag                                                                    1622


<210>  77
<211>  1626
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SERPINA1 transcript comprising SEQ 69

<400>  77
gggtcccgca gtcggcgtcc agcggctctg cttgttcgtg tgtgtgtcgt tgcaggcctt       60

attcggatca gccaccatgc catcttctgt ctcttggggt atcttgttgt tggccggttt      120

gtgctgcttg gtcccagtct ctttggccga agacccacaa ggtgacgccg cccaaaagac      180

cgacacctct caccacgacc aagaccaccc aaccttcaac aagatcaccc caaacttggc      240

cgaattcgcc ttctctttgt acagacaatt ggcccaccaa tctaactcta ccaacatctt      300

cttctctcca gtctctatcg ccaccgcctt cgccatgttg tctttgggta ccaaggccga      360

cacccacgac gaaatcttgg aaggtttgaa cttcaacttg accgaaatcc cagaagccca      420

aatccacgaa ggtttccaag aattgttgag aaccttgaac caaccagact ctcaattgca      480

attgaccacc ggtaacggtt tgttcttgtc tgaaggtttg aagttggtcg acaagttctt      540

ggaagacgtc aagaagttgt accactctga agccttcacc gtcaacttcg gtgacaccga      600

agaagccaag aagcaaatca acgactacgt cgaaaagggt acccaaggta agatcgtcga      660

cttggtcaag gaattggaca gagacaccgt cttcgccttg gtcaactaca tcttcttcaa      720

gggtaagtgg gaaagaccat tcgaagtcaa ggacaccgaa gaagaagact tccacgtcga      780

ccaagtcacc accgtcaagg tcccaatgat gaagagattg ggtatgttca acatccaaca      840

ctgcaagaag ttgtcttctt gggtcttgtt gatgaagtac ttgggtaacg ccaccgccat      900

cttcttcttg ccagacgaag gtaagttgca acacttggaa aacgaattga cccacgacat      960

catcaccaag ttcttggaaa acgaagacag aagatctgcc tctttgcact tgccaaagtt     1020

gtctatcacc ggtacctacg acttgaagtc tgtcttgggt caattgggta tcaccaaggt     1080

cttctctaac ggtgccgact tgtctggtgt caccgaagaa gccccattga agttgtctaa     1140

ggccgtccac aaggccgtct tgaccatcga cgaaaagggt accgaagccg ccggtgccat     1200

gttcttggaa gccatcccaa tgtctatccc accagaagtc aagttcaaca agccattcgt     1260

cttcttgatg atcgaacaaa acaccaagtc tccattgttc atgggtaagg tcgtcaaccc     1320

aacccaaaag tagctagcca tcacatttaa aagcatctca gcctaccatg agaataagag     1380

aaagaaaatg aagatcaata gcttattcat ctctttttct ttttcgttgg tgtaaagcca     1440

acaccctgtc taaaaaacat aaatttcttt aatcattttg cctcttttct ctgtgcttca     1500

attaataaaa aatggaaaga acctcgagaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1620

atctag                                                                1626


<210>  78
<211>  1640
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SERPINA1 transcript comprising SEQ 71

<400>  78
gggtcccgca gtcggcgtcc agcggctctg cttgttcgtg tgtgtgtcgt tgcaggcctt       60

attccgccac catgcccagc agcgtgagct ggggcatcct gctgctggcc ggcctgtgct      120

gcctggtgcc cgtgagcctg gccgaggacc cccagggcga cgccgcccag aagacggaca      180

cgagccacca cgaccaggac caccccacgt tcaacaagat cacgcccaac ctggccgagt      240

tcgccttcag cctgtaccgg cagctggccc accagagcaa cagcacgaac atcttcttca      300

gccccgtgag catcgccacg gccttcgcca tgctgagcct gggcacgaag gccgacacgc      360

acgacgagat cctggagggc ctgaacttca acctgacgga gatccccgag gcccagatcc      420

acgagggctt ccaggagctg ctgcggacgc tgaaccagcc cgacagccag ctgcagctga      480

cgacgggcaa cggcctgttc ctgagcgagg gcctgaagct ggtggacaag ttcctggagg      540

acgtgaagaa gctgtaccac agcgaggcct tcacggtgaa cttcggcgac acggaggagg      600

ccaagaagca gatcaacgac tacgtggaga agggcacgca gggcaagatc gtggacctgg      660

tgaaggagct ggaccgggac acggtgttcg ccctggtgaa ctacatcttc ttcaagggca      720

agtgggagcg gcccttcgag gtgaaggaca cggaggagga ggacttccac gtggaccagg      780

tgacgacggt gaaggtgccc atgatgaagc ggctgggcat gttcaacatc cagcactgca      840

agaagctgag cagctgggtg ctgctgatga agtacctggg caacgccacg gccatcttct      900

tcctgcccga cgagggcaag ctgcagcacc tggagaacga gctgacgcac gacatcatca      960

cgaagttcct ggagaacgag gaccggcgga gcgccagcct gcacctgccc aagctgagca     1020

tcacgggcac gtacgacctg aagagcgtgc tgggccagct gggcatcacg aaggtgttca     1080

gcaacggcgc cgacctgagc ggcgtgacgg aggaggcccc cctgaagctg agcaaggccg     1140

tgcacaaggc cgtgctgacg atcgacgaga agggcacgga ggccgccggc gccatgttcc     1200

tggaggccat ccccatgagc atcccccccg aggtgaagtt caacaagccc ttcgtgttcc     1260

tgatgatcga gcagaacacg aagagccccc tgttcatggg caaggtggtg aaccccacgc     1320

agaagtagta gtgaagcttc tagccatcac atttaaaagc atctcagcct accatgagaa     1380

taagagaaag aaaatgaaga tcaatagctt attcatctct ttttcttttt cgttggtgta     1440

aagccaacac cctgtctaaa aaacataaat ttctttaatc attttgcctc ttttctctgt     1500

gcttcaatta ataaaaaatg gaaagaacct cgagaaaaaa aaaaaaaaaa aaaaaaaaaa     1560

aaaagcgaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaccg aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaat                                                 1640


<210>  79
<211>  1640
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SERPINA1 transcript comprising SEQ 72

<400>  79
gggtcccgca gtcggcgtcc agcggctctg cttgttcgtg tgtgtgtcgt tgcaggcctt       60

attccgccac catgccctcg tcggtctcgt ggggcatcct cctcctcgcg ggcctctgct      120

gcctcgtccc cgtctcgctc gcggaggacc cccagggcga cgcggcgcag aagacggaca      180

cgtcgcacca cgaccaggac caccccacgt tcaacaagat cacgcccaac ctcgcggagt      240

tcgcgttctc gctctaccgc cagctcgcgc accagtcgaa ctcgacgaac atcttcttct      300

cgcccgtctc gatcgcgacg gcgttcgcga tgctctcgct cggcacgaag gcggacacgc      360

acgacgagat cctcgagggc ctcaacttca acctcacgga gatccccgag gcgcagatcc      420

acgagggctt ccaggagctc ctccgcacgc tcaaccagcc cgactcgcag ctccagctca      480

cgacgggcaa cggcctcttc ctctcggagg gcctcaagct cgtcgacaag ttcctcgagg      540

acgtcaagaa gctctaccac tcggaggcgt tcacggtcaa cttcggcgac acggaggagg      600

cgaagaagca gatcaacgac tacgtcgaga agggcacgca gggcaagatc gtcgacctcg      660

tcaaggagct cgaccgcgac acggtcttcg cgctcgtcaa ctacatcttc ttcaagggca      720

agtgggagcg ccccttcgag gtcaaggaca cggaggagga ggacttccac gtcgaccagg      780

tcacgacggt caaggtcccc atgatgaagc gcctcggcat gttcaacatc cagcactgca      840

agaagctctc gtcgtgggtc ctcctcatga agtacctcgg caacgcgacg gcgatcttct      900

tcctccccga cgagggcaag ctccagcacc tcgagaacga gctcacgcac gacatcatca      960

cgaagttcct cgagaacgag gaccgccgct cggcgtcgct ccacctcccc aagctctcga     1020

tcacgggcac gtacgacctc aagtcggtcc tcggccagct cggcatcacg aaggtcttct     1080

cgaacggcgc ggacctctcg ggcgtcacgg aggaggcgcc cctcaagctc tcgaaggcgg     1140

tccacaaggc ggtcctcacg atcgacgaga agggcacgga ggcggcgggc gcgatgttcc     1200

tcgaggcgat ccccatgtcg atcccccccg aggtcaagtt caacaagccc ttcgtcttcc     1260

tcatgatcga gcagaacacg aagtcgcccc tcttcatggg caaggtcgtc aaccccacgc     1320

agaagtagta gtagagcttc tagccatcac atttaaaagc atctcagcct accatgagaa     1380

taagagaaag aaaatgaaga tcaatagctt attcatctct ttttcttttt cgttggtgta     1440

aagccaacac cctgtctaaa aaacataaat ttctttaatc attttgcctc ttttctctgt     1500

gcttcaatta ataaaaaatg gaaagaacct cgagaaaaaa aaaaaaaaaa aaaaaaaaaa     1560

aaaagcgaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaccg aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaat                                                 1640


<210>  80
<211>  1640
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SERPINA1 transcript comprising SEQ 73

<400>  80
gggtcccgca gtcggcgtcc agcggctctg cttgttcgtg tgtgtgtcgt tgcaggcctt       60

attccgccac catgccctca tcggtcagct ggggcatcct cctcctcgcc gggctctgct      120

gcctcgttcc cgtcagcctc gcggaggacc cccagggcga cgctgcccag aagacggaca      180

cgtcgcacca cgaccaggac caccccacct tcaacaagat cactcccaat ctcgcggagt      240

tcgcgttctc gctctaccgc cagctcgcgc accagagcaa ctcgactaac atcttcttct      300

cgcccgtcag catcgcgacg gcgttcgcga tgctcagcct cggcacgaag gcggacacgc      360

acgacgagat cctcgagggc ctcaacttca atctcacaga gatcccagaa gcccagatcc      420

acgagggctt ccaggagctg ctgcggacgc tcaaccagcc tgactcgcag ctccagctca      480

cgacgggcaa tgggctcttc ctcagcgagg gcctcaagct cgtcgacaag ttcctggagg      540

acgtcaagaa gctctaccac tcggaagcct tcacggtcaa cttcggcgac acagaggaag      600

ccaagaagca gatcaacgac tacgtcgaga aggggactca gggcaagatc gtcgacctcg      660

tcaaggagct ggaccgagac acggtcttcg cactggtcaa ctacatcttc ttcaagggga      720

agtgggagcg ccccttcgaa gtcaaggaca cagaggagga ggacttccac gtcgaccagg      780

tgacgacggt caaggttccc atgatgaagc gcctcggcat gttcaacatc cagcactgca      840

agaagctcag ctcgtgggtc ctcctcatga agtacctcgg caacgcgacg gcgatcttct      900

tccttcctga cgagggcaag ctccagcacc tcgagaacga gctgacgcac gacatcatca      960

cgaagttcct ggagaacgag gaccgccgat cggcgtcgct ccaccttcca aagctcagca     1020

tcacgggcac ctacgacctc aagtcggtcc tcggccagct cggcatcacg aaggtcttct     1080

cgaatggtgc cgacctcagc ggcgtcacag aggaagcccc cctcaagctc agcaaggctg     1140

tgcacaaggc tgtgctcacg atcgacgaga aggggacaga agctgccggt gccatgttcc     1200

tggaagccat ccccatgagc atcccaccag aagtcaagtt caacaagccc ttcgtcttcc     1260

tgatgataga gcagaacacg aagtcgcccc tcttcatggg caaggtcgtc aaccccactc     1320

aaaagtagtg atagagcttc tagccatcac atttaaaagc atctcagcct accatgagaa     1380

taagagaaag aaaatgaaga tcaatagctt attcatctct ttttcttttt cgttggtgta     1440

aagccaacac cctgtctaaa aaacataaat ttctttaatc attttgcctc ttttctctgt     1500

gcttcaatta ataaaaaatg gaaagaacct cgagaaaaaa aaaaaaaaaa aaaaaaaaaa     1560

aaaagcgaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaccg aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaat                                                 1640


<210>  81

<400>  81
000

<210>  82

<400>  82
000

<210>  83

<400>  83
000

<210>  84

<400>  84
000

<210>  85

<400>  85
000

<210>  86

<400>  86
000

<210>  87

<400>  87
000

<210>  88
<211>  418
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: FAH amino acid sequence

<400>  88

Met Ser Phe Ile Pro Val Ala Glu Asp Ser Asp Phe Pro Ile His Asn 
1               5                   10                  15      


Leu Pro Tyr Gly Val Phe Ser Thr Arg Gly Asp Pro Arg Pro Arg Ile 
            20                  25                  30          


Gly Val Ala Ile Gly Asp Gln Ile Leu Asp Leu Ser Ile Ile Lys His 
        35                  40                  45              


Leu Phe Thr Gly Pro Val Leu Ser Lys His Gln Asp Val Phe Asn Gln 
    50                  55                  60                  


Pro Thr Leu Asn Ser Phe Met Gly Leu Gly Gln Ala Ala Trp Lys Glu 
65                  70                  75                  80  


Ala Arg Val Phe Leu Gln Asn Leu Leu Ser Val Ser Gln Ala Arg Leu 
                85                  90                  95      


Arg Asp Asp Thr Glu Leu Arg Lys Cys Ala Phe Ile Ser Gln Ala Ser 
            100                 105                 110         


Ala Thr Met His Leu Pro Ala Thr Ile Gly Asp Tyr Thr Asp Phe Tyr 
        115                 120                 125             


Ser Ser Arg Gln His Ala Thr Asn Val Gly Ile Met Phe Arg Asp Lys 
    130                 135                 140                 


Glu Asn Ala Leu Met Pro Asn Trp Leu His Leu Pro Val Gly Tyr His 
145                 150                 155                 160 


Gly Arg Ala Ser Ser Val Val Val Ser Gly Thr Pro Ile Arg Arg Pro 
                165                 170                 175     


Met Gly Gln Met Lys Pro Asp Asp Ser Lys Pro Pro Val Tyr Gly Ala 
            180                 185                 190         


Cys Lys Leu Leu Asp Met Glu Leu Glu Met Ala Phe Phe Val Gly Pro 
        195                 200                 205             


Gly Asn Arg Leu Gly Glu Pro Ile Pro Ile Ser Lys Ala His Glu His 
    210                 215                 220                 


Ile Phe Gly Met Val Leu Met Asn Asp Trp Ser Ala Arg Asp Ile Gln 
225                 230                 235                 240 


Lys Trp Glu Tyr Val Pro Leu Gly Pro Phe Leu Gly Lys Ser Phe Gly 
                245                 250                 255     


Thr Thr Val Ser Pro Trp Val Val Pro Met Asp Ala Leu Met Pro Phe 
            260                 265                 270         


Ala Val Pro Asn Pro Lys Gln Asp Pro Arg Pro Leu Pro Tyr Leu Cys 
        275                 280                 285             


His Asp Glu Pro Tyr Thr Phe Asp Ile Asn Leu Ser Val Asn Leu Lys 
    290                 295                 300                 


Gly Glu Gly Met Ser Gln Ala Ala Thr Ile Cys Lys Ser Asn Phe Lys 
305                 310                 315                 320 


Tyr Met Tyr Trp Thr Met Leu Gln Gln Leu Thr His His Ser Val Asn 
                325                 330                 335     


Gly Cys Asn Leu Arg Pro Gly Asp Leu Leu Ala Ser Gly Thr Ile Ser 
            340                 345                 350         


Gly Pro Glu Pro Glu Asn Phe Gly Ser Met Leu Glu Leu Ser Trp Lys 
        355                 360                 365             


Gly Thr Lys Pro Ile Asp Leu Gly Asn Gly Gln Thr Arg Lys Phe Leu 
    370                 375                 380                 


Leu Asp Gly Asp Glu Val Ile Ile Thr Gly Tyr Cys Gln Gly Asp Gly 
385                 390                 395                 400 


Tyr Arg Ile Gly Phe Gly Gln Cys Ala Gly Lys Val Leu Pro Ala Leu 
                405                 410                 415     


Leu Pro 
        


<210>  89
<211>  1257
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: FAH WT ORF

<400>  89
augucuuuua uuccuguugc ugaagauucu gauuuuccua uucauaauuu accuuauggu       60

guuuuuucua cucgugguga uccucguccu cguauuggug uugcuauugg ugaucaaauu      120

uuagauuuau cuauuauuaa acauuuauuu acugguccug uuuuaucuaa acaucaagau      180

guuuuuaauc aaccuacuuu aaauucuuuu auggguuuag gucaagcugc uuggaaagaa      240

gcucguguuu uuuuacaaaa uuuauuaucu guuucucaag cucguuuacg ugaugauacu      300

gaauuacgua aaugugcuuu uauuucucaa gcuucugcua cuaugcauuu accugcuacu      360

auuggugauu auacugauuu uuauucuucu cgucaacaug cuacuaaugu ugguauuaug      420

uuucgugaua aagaaaaugc uuuaaugccu aauugguuac auuuaccugu ugguuaucau      480

ggucgugcuu cuucuguugu uguuucuggu acuccuauuc gucguccuau gggucaaaug      540

aaaccugaug auucuaaacc uccuguuuau ggugcuugua aauuauuaga uauggaauua      600

gaaauggcuu uuuuuguugg uccugguaau cguuuaggug aaccuauucc uauuucuaaa      660

gcucaugaac auauuuuugg uaugguuuua augaaugauu ggucugcucg ugauauucaa      720

aaaugggaau auguuccuuu agguccuuuu uuagguaaau cuuuugguac uacuguuucu      780

ccuuggguug uuccuaugga ugcuuuaaug ccuuuugcug uuccuaaucc uaaacaagau      840

ccucguccuu uaccuuauuu augucaugau gaaccuuaua cuuuugauau uaauuuaucu      900

guuaauuuaa aaggugaagg uaugucucaa gcugcuacua uuuguaaauc uaauuuuaaa      960

uauauguauu ggacuauguu acaacaauua acucaucauu cuguuaaugg uuguaauuua     1020

cguccuggug auuuauuagc uucugguacu auuucugguc cugaaccuga aaauuuuggu     1080

ucuauguuag aauuaucuug gaaagguacu aaaccuauug auuuagguaa uggucaaacu     1140

cguaaauuuu uauuagaugg ugaugaaguu auuauuacug guuauuguca aggugauggu     1200

uaucguauug guuuugguca augugcuggu aaaguuuuac cugcuuuauu accuuag        1257


<210>  90
<211>  1257
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: FAH BP_GCU ORF

<400>  90
atgtcgttca tccccgtcgc ggaggactcg gacttcccca tccacaacct cccctacggc       60

gtcttctcga cgcgcggcga cccccgcccc cgcatcggcg tcgcgatcgg cgaccagatc      120

ctcgacctct cgatcatcaa gcacctcttc acgggccccg tcctctcgaa gcaccaggac      180

gtcttcaacc agcccacgct caactcgttc atgggcctcg gccaggcggc gtggaaggag      240

gcgcgcgtct tcctccagaa cctcctctcg gtctcgcagg cgcgcctccg cgacgacacg      300

gagctccgca agtgcgcgtt catctcgcag gcgtcggcga cgatgcacct ccccgcgacg      360

atcggcgact acacggactt ctactcgtcg cgccagcacg cgacgaacgt cggcatcatg      420

ttccgcgaca aggagaacgc gctcatgccc aactggctcc acctccccgt cggctaccac      480

ggccgcgcgt cgtcggtcgt cgtctcgggc acgcccatcc gccgccccat gggccagatg      540

aagcccgacg actcgaagcc ccccgtctac ggcgcgtgca agctcctcga catggagctc      600

gagatggcgt tcttcgtcgg ccccggcaac cgcctcggcg agcccatccc catctcgaag      660

gcgcacgagc acatcttcgg catggtcctc atgaacgact ggtcggcgcg cgacatccag      720

aagtgggagt acgtccccct cggccccttc ctcggcaagt cgttcggcac gacggtctcg      780

ccctgggtcg tccccatgga cgcgctcatg cccttcgcgg tccccaaccc caagcaggac      840

ccccgccccc tcccctacct ctgccacgac gagccctaca cgttcgacat caacctctcg      900

gtcaacctca agggcgaggg catgtcgcag gcggcgacga tctgcaagtc gaacttcaag      960

tacatgtact ggacgatgct ccagcagctc acgcaccact cggtcaacgg ctgcaacctc     1020

cgccccggcg acctcctcgc gtcgggcacg atctcgggcc ccgagcccga gaacttcggc     1080

tcgatgctcg agctctcgtg gaagggcacg aagcccatcg acctcggcaa cggccagacg     1140

cgcaagttcc tcctcgacgg cgacgaggtc atcatcacgg gctactgcca gggcgacggc     1200

taccgcatcg gcttcggcca gtgcgcgggc aaggtcctcc ccgcgctcct cccctag        1257


<210>  91
<211>  1260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: FAH GP_BP_BS_GCU ORF

<400>  91
atgtcgttca tccccgtcgc ggaggactcg gacttcccca tccacaatct tccatacggc       60

gtcttctcga ctcgcggcga cccccgcccc cgcatcggcg tcgcgatcgg cgaccagatc      120

ctcgacctca gcatcatcaa gcacctcttc acgggccccg tcctcagcaa gcaccaggac      180

gtcttcaacc agcccaccct caactcgttc atgggcctcg gccaggctgc ctggaaggaa      240

gcccgcgtct tcctgcagaa tctcctcagc gtcagccagg cccgcctgcg agacgacaca      300

gagctgcgga agtgcgcgtt catctcgcag gcctcggcga cgatgcacct tccagcgacg      360

atcggcgact acacggactt ctactcgtcg cgccagcacg cgacgaacgt tggcatcatg      420

ttccgagaca aggagaacgc actgatgccc aactggctcc accttccagt tggctaccac      480

ggccgcgcgt cgtcggtcgt cgtcagcggc actcccatcc gccgccccat gggccagatg      540

aagcctgacg actcgaagcc acccgtctac ggtgcctgca agctcctcga catggagctg      600

gagatggcgt tcttcgttgg ccctggcaac cgcctcggtg agcccatccc catctcgaag      660

gcgcacgagc acatcttcgg catggtcctc atgaacgact ggtcggcccg agacatccag      720

aagtgggagt acgttcccct cggccccttc ctgggcaagt cgttcggcac gacggtcagc      780

ccctgggtcg ttcccatgga cgcactgatg cccttcgctg ttcccaaccc caagcaggac      840

ccccgccccc ttccatacct ctgccacgac gagccctaca cgttcgacat caatctcagc      900

gtcaatctca agggtgaggg catgtcgcag gctgccacga tctgcaagtc gaacttcaag      960

tacatgtact ggacgatgct ccagcagctc acgcaccact cggtcaatgg gtgcaatctg     1020

cggcctggcg acctcctcgc gtcgggcacg atctcgggcc cagagccaga gaacttcggc     1080

tcgatgctcg agctcagctg gaaggggacg aagcccatcg acctcggcaa tgggcagacg     1140

cgcaagttcc tgctcgacgg cgacgaagtc atcatcacgg gctactgcca gggcgacggc     1200

taccgcatcg gcttcggcca gtgcgccggg aaggtccttc cagcactgct tccctcatag     1260


<210>  92
<211>  1260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: FAH GS_BS_GCU ORF

<400>  92
atgagcttca tccccgtcgc ggaggacagc gacttcccca tccacaatct tccatacggc       60

gtcttctcga ctcgcggcga cccccgcccc cgcatcggcg tcgcgatcgg cgaccagatc      120

ctcgacctca gcatcatcaa gcacctcttc acgggccccg tcctcagcaa gcaccaggac      180

gtcttcaacc agcccaccct caacagcttc atgggcctcg gccaggctgc ctggaaggaa      240

gcccgcgtct tcctgcagaa tctcctcagc gtcagccagg cccgcctgcg agacgacaca      300

gagctgcgga agtgcgcgtt catcagccag gccagcgcga cgatgcacct tccagcgacg      360

atcggcgact acacggactt ctacagcagc cgccagcacg cgacgaacgt tggcatcatg      420

ttccgagaca aggagaacgc actgatgccc aactggctcc accttccagt tggctaccac      480

ggccgcgcga gcagcgtcgt cgtcagcggc actcccatcc gccgccccat gggccagatg      540

aagcctgacg acagcaagcc acccgtctac ggtgcctgca agctcctcga catggagctg      600

gagatggcgt tcttcgttgg ccctggcaac cgcctcggtg agcccatccc catcagcaag      660

gcgcacgagc acatcttcgg catggtcctc atgaacgact ggagcgcccg agacatccag      720

aagtgggagt acgttcccct cggccccttc ctgggcaaga gcttcggcac gacggtcagc      780

ccctgggtcg ttcccatgga cgcactgatg cccttcgctg ttcccaaccc caagcaggac      840

ccccgccccc ttccatacct ctgccacgac gagccctaca cgttcgacat caatctcagc      900

gtcaatctca agggtgaggg catgagccag gctgccacga tctgcaagag caacttcaag      960

tacatgtact ggacgatgct ccagcagctc acgcaccaca gcgtcaatgg gtgcaatctg     1020

cggcctggcg acctcctcgc gagcggcacg atcagcggcc cagagccaga gaacttcggc     1080

agcatgctcg agctcagctg gaaggggacg aagcccatcg acctcggcaa tgggcagacg     1140

cgcaagttcc tgctcgacgg cgacgaagtc atcatcacgg gctactgcca gggcgacggc     1200

taccgcatcg gcttcggcca gtgcgccggg aaggtccttc cagcactgct tccctcatag     1260


<210>  93
<211>  1260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: FAH GS_GCU ORF

<400>  93
atgagcttca tccccgtggc ggaggacagc gacttcccga tccacaatct tccatacggc       60

gtgttctcga ctcgcggcga cccgcgcccg cgcatcggcg tggcgatcgg cgaccagatc      120

ctggacctca gcatcatcaa gcacctgttc acgggcccgg tgctcagcaa gcaccaggac      180

gtgttcaacc agcccaccct gaacagcttc atgggcctgg gccaggctgc ctggaaggaa      240

gcccgcgtgt tcctgcagaa tctcctcagc gtcagccagg cccgcctgcg agacgacaca      300

gagctgcgga agtgcgcgtt catcagccag gccagcgcga cgatgcacct tccagcgacg      360

atcggcgact acacggactt ctacagcagc cgccagcacg cgacgaacgt tggcatcatg      420

ttccgagaca aggagaacgc actgatgccg aactggctgc accttccagt tggctaccac      480

ggccgcgcga gcagcgtggt ggtcagcggc actcccatcc gccgcccgat gggccagatg      540

aagcctgacg acagcaagcc acccgtgtac ggtgcctgca agctgctgga catggagctg      600

gagatggcgt tcttcgttgg cccgggcaac cgcctgggtg agccgatccc catcagcaag      660

gcgcacgagc acatcttcgg catggtgctg atgaacgact ggagcgcccg agacatccag      720

aagtgggagt acgttcccct gggcccgttc ctgggcaaga gcttcggcac gacggtcagc      780

ccgtgggtgg ttcccatgga cgcactgatg ccgttcgctg ttcccaaccc gaagcaggac      840

ccgcgcccgc ttccatacct gtgccacgac gagccgtaca cgttcgacat caatctcagc      900

gtgaatctca agggtgaggg catgagccag gctgccacga tctgcaagag caacttcaag      960

tacatgtact ggacgatgct gcagcagctg acgcaccaca gcgtgaatgg gtgcaatctg     1020

cggccgggcg acctgctggc gagcggcacg atcagcggcc cagagccaga gaacttcggc     1080

agcatgctgg agctcagctg gaaggggacg aagccgatcg acctgggcaa tgggcagacg     1140

cgcaagttcc tgctggacgg cgacgaagtc atcatcacgg gctactgcca gggcgacggc     1200

taccgcatcg gcttcggcca gtgcgccggg aaggtgcttc cagcactgct tccctcatag     1260


<210>  94
<211>  464
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: GABRD amino acid sequence

<400>  94

Met Ser Glu Ala Thr Pro Leu Asp Arg Asn Asp Ser Glu Asn Thr Gly 
1               5                   10                  15      


Gly Leu Ile Ser Arg Pro His Pro Trp Asp Gln Ser Pro Ser Cys Val 
            20                  25                  30          


Gln Glu Asp Arg Ala Met Asn Asp Ile Gly Asp Tyr Val Gly Ser Asn 
        35                  40                  45              


Leu Glu Ile Ser Trp Leu Pro Asn Leu Asp Gly Leu Ile Ala Gly Tyr 
    50                  55                  60                  


Ala Arg Asn Phe Arg Pro Gly Ile Gly Gly Pro Pro Val Asn Val Ala 
65                  70                  75                  80  


Leu Ala Leu Glu Val Ala Ser Ile Asp His Ile Ser Glu Ala Asn Met 
                85                  90                  95      


Glu Tyr Thr Met Thr Val Phe Leu His Gln Ser Trp Arg Asp Ser Arg 
            100                 105                 110         


Leu Ser Tyr Asn His Thr Asn Glu Thr Leu Gly Leu Asp Ser Arg Phe 
        115                 120                 125             


Val Asp Lys Leu Trp Leu Pro Asp Thr Phe Ile Val Asn Ala Lys Ser 
    130                 135                 140                 


Ala Trp Phe His Asp Val Thr Val Glu Asn Lys Leu Ile Arg Leu Gln 
145                 150                 155                 160 


Pro Asp Gly Val Ile Leu Tyr Ser Ile Arg Ile Thr Ser Thr Val Ala 
                165                 170                 175     


Cys Asp Met Asp Leu Ala Lys Tyr Pro Met Asp Glu Gln Glu Cys Met 
            180                 185                 190         


Leu Asp Leu Glu Ser Tyr Gly Tyr Ser Ser Glu Asp Ile Val Tyr Tyr 
        195                 200                 205             


Trp Ser Glu Ser Gln Glu His Ile His Gly Leu Asp Lys Leu Gln Leu 
    210                 215                 220                 


Ala Gln Phe Thr Ile Thr Ser Tyr Arg Phe Thr Thr Glu Leu Met Asn 
225                 230                 235                 240 


Phe Lys Ser Ala Gly Gln Phe Pro Arg Leu Ser Leu His Phe His Leu 
                245                 250                 255     


Arg Arg Asn Arg Gly Val Tyr Ile Ile Gln Ser Tyr Met Pro Ser Val 
            260                 265                 270         


Leu Leu Val Ala Met Ser Trp Val Ser Phe Trp Ile Ser Gln Ala Ala 
        275                 280                 285             


Val Pro Ala Arg Val Ser Leu Gly Ile Thr Thr Val Leu Thr Met Thr 
    290                 295                 300                 


Thr Leu Met Val Ser Ala Arg Ser Ser Leu Pro Arg Ala Ser Ala Ile 
305                 310                 315                 320 


Lys Ala Leu Asp Val Tyr Phe Trp Ile Cys Tyr Val Phe Val Phe Ala 
                325                 330                 335     


Ala Leu Val Glu Tyr Ala Phe Ala His Phe Asn Ala Asp Tyr Arg Lys 
            340                 345                 350         


Lys Gln Lys Ala Lys Val Lys Val Ser Arg Pro Arg Ala Glu Met Asp 
        355                 360                 365             


Val Arg Asn Ala Ile Val Leu Phe Ser Leu Ser Ala Ala Gly Val Thr 
    370                 375                 380                 


Gln Glu Leu Ala Ile Ser Arg Arg Gln Arg Arg Val Pro Gly Asn Leu 
385                 390                 395                 400 


Met Gly Ser Tyr Arg Ser Val Gly Val Glu Thr Gly Glu Thr Lys Lys 
                405                 410                 415     


Glu Gly Ala Ala Arg Ser Gly Gly Gln Gly Gly Ile Arg Ala Arg Leu 
            420                 425                 430         


Arg Pro Ile Asp Ala Asp Thr Ile Asp Ile Tyr Ala Arg Ala Val Phe 
        435                 440                 445             


Pro Ala Ala Phe Ala Ala Val Asn Val Ile Tyr Trp Ala Ala Tyr Ala 
    450                 455                 460                 


<210>  95
<211>  1395
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GABRD WT ORF

<400>  95
augucugaag cuacuccuuu agaucguaau gauucugaaa auacuggugg uuuaauuucu       60

cguccucauc cuugggauca aucuccuucu uguguucaag aagaucgugc uaugaaugau      120

auuggugauu auguugguuc uaauuuagaa auuucuuggu uaccuaauuu agaugguuua      180

auugcugguu augcucguaa uuuucguccu gguauuggug guccuccugu uaauguugcu      240

uuagcuuuag aaguugcuuc uauugaucau auuucugaag cuaauaugga auauacuaug      300

acuguuuuuu uacaucaauc uuggcgugau ucucguuuau cuuauaauca uacuaaugaa      360

acuuuagguu uagauucucg uuuuguugau aaauuauggu uaccugauac uuuuauuguu      420

aaugcuaaau cugcuugguu ucaugauguu acuguugaaa auaaauuaau ucguuuacaa      480

ccugauggug uuauuuuaua uucuauucgu auuacuucua cuguugcuug ugauauggau      540

uuagcuaaau auccuaugga ugaacaagaa uguauguuag auuuagaauc uuaugguuau      600

ucuucugaag auauuguuua uuauuggucu gaaucucaag aacauauuca ugguuuagau      660

aaauuacaau uagcucaauu uacuauuacu ucuuaucguu uuacuacuga auuaaugaau      720

uuuaaaucug cuggucaauu uccucguuua ucuuuacauu uucauuuacg ucguaaucgu      780

gguguuuaua uuauucaauc uuauaugccu ucuguuuuau uaguugcuau gucuuggguu      840

ucuuuuugga uuucucaagc ugcuguuccu gcucguguuu cuuuagguau uacuacuguu      900

uuaacuauga cuacuuuaau gguuucugcu cguucuucuu uaccucgugc uucugcuauu      960

aaagcuuuag auguuuauuu uuggauuugu uauguuuuug uuuuugcugc uuuaguugaa     1020

uaugcuuuug cucauuuuaa ugcugauuau cguaaaaaac aaaaagcuaa aguuaaaguu     1080

ucucguccuc gugcugaaau ggauguucgu aaugcuauug uuuuauuuuc uuuaucugcu     1140

gcugguguua cucaagaauu agcuauuucu cgucgucaac gucguguucc ugguaauuua     1200

auggguucuu aucguucugu ugguguugaa acuggugaaa cuaaaaaaga aggugcugcu     1260

cguucuggug gucaaggugg uauucgugcu cguuuacguc cuauugaugc ugauacuauu     1320

gauauuuaug cucgugcugu uuuuccugcu gcuuuugcug cuguuaaugu uauuuauugg     1380

gcugcuuaug cuuag                                                      1395


<210>  96
<211>  1395
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GABRD BP_GCU ORF

<400>  96
atgtcggagg cgacgcccct cgaccgcaac gactcggaga acacgggcgg cctcatctcg       60

cgcccccacc cctgggacca gtcgccctcg tgcgtccagg aggaccgcgc gatgaacgac      120

atcggcgact acgtcggctc gaacctcgag atctcgtggc tccccaacct cgacggcctc      180

atcgcgggct acgcgcgcaa cttccgcccc ggcatcggcg gcccccccgt caacgtcgcg      240

ctcgcgctcg aggtcgcgtc gatcgaccac atctcggagg cgaacatgga gtacacgatg      300

acggtcttcc tccaccagtc gtggcgcgac tcgcgcctct cgtacaacca cacgaacgag      360

acgctcggcc tcgactcgcg cttcgtcgac aagctctggc tccccgacac gttcatcgtc      420

aacgcgaagt cggcgtggtt ccacgacgtc acggtcgaga acaagctcat ccgcctccag      480

cccgacggcg tcatcctcta ctcgatccgc atcacgtcga cggtcgcgtg cgacatggac      540

ctcgcgaagt accccatgga cgagcaggag tgcatgctcg acctcgagtc gtacggctac      600

tcgtcggagg acatcgtcta ctactggtcg gagtcgcagg agcacatcca cggcctcgac      660

aagctccagc tcgcgcagtt cacgatcacg tcgtaccgct tcacgacgga gctcatgaac      720

ttcaagtcgg cgggccagtt cccccgcctc tcgctccact tccacctccg ccgcaaccgc      780

ggcgtctaca tcatccagtc gtacatgccc tcggtcctcc tcgtcgcgat gtcgtgggtc      840

tcgttctgga tctcgcaggc ggcggtcccc gcgcgcgtct cgctcggcat cacgacggtc      900

ctcacgatga cgacgctcat ggtctcggcg cgctcgtcgc tcccccgcgc gtcggcgatc      960

aaggcgctcg acgtctactt ctggatctgc tacgtcttcg tcttcgcggc gctcgtcgag     1020

tacgcgttcg cgcacttcaa cgcggactac cgcaagaagc agaaggcgaa ggtcaaggtc     1080

tcgcgccccc gcgcggagat ggacgtccgc aacgcgatcg tcctcttctc gctctcggcg     1140

gcgggcgtca cgcaggagct cgcgatctcg cgccgccagc gccgcgtccc cggcaacctc     1200

atgggctcgt accgctcggt cggcgtcgag acgggcgaga cgaagaagga gggcgcggcg     1260

cgctcgggcg gccagggcgg catccgcgcg cgcctccgcc ccatcgacgc ggacacgatc     1320

gacatctacg cgcgcgcggt cttccccgcg gcgttcgcgg cggtcaacgt catctactgg     1380

gcggcgtacg cgtag                                                      1395


<210>  97
<211>  1395
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GABRD GP_BP_BS_GCU ORF

<400>  97
atgtcggaag ccactcccct cgaccgcaac gactcggaga acacgggtgg cctcatctcg       60

cgcccccacc cctgggacca gagcccctca tgcgtccagg aggaccgcgc gatgaacgac      120

atcggcgact acgttggctc gaatctcgag atctcgtggc ttccaaatct cgacggcctc      180

atcgccgggt acgcccgcaa cttccgccct ggcatcggtg gcccacccgt caacgtcgca      240

ctggcactgg aagtcgcgag catcgaccac atctcggaag ccaacatgga gtacacgatg      300

acggtcttcc tgcaccagag ctggcgagac tcgcgcctca gctacaacca cacgaacgag      360

acgctcggcc tcgactcgcg cttcgtcgac aagctctggc ttcctgacac gttcatcgtc      420

aacgcgaagt cggcgtggtt ccacgacgtc acggtcgaga acaagctcat ccgcctccag      480

cctgacggcg tcatcctcta cagcatccgc atcacgtcga ctgtcgcgtg cgacatggac      540

ctcgcgaagt accccatgga cgagcaggag tgcatgctcg acctcgagtc gtacggctac      600

tcgtcggagg acatcgtcta ctactggtcg gagtcgcagg agcacatcca cggcctcgac      660

aagctccagc tcgcgcagtt cacgatcacg tcgtaccgct tcacgacaga gctgatgaac      720

ttcaagtcgg ccgggcagtt cccccgcctc agcctccact tccacctgcg gcgcaaccgc      780

ggcgtctaca tcatccagag ctacatgccc tcagtcctcc tcgtcgcgat gtcgtgggtc      840

agcttctgga tctcgcaggc tgctgttccc gcccgcgtca gcctcggcat cacgacggtc      900

ctcacgatga cgacgctcat ggtcagcgcc cgctcgtcgc ttccacgcgc gtcggcgatc      960

aaggcactgg acgtctactt ctggatctgc tacgtcttcg tcttcgctgc actggtcgag     1020

tacgcgttcg cgcacttcaa cgcggactac cgcaagaagc agaaggcgaa ggtcaaggtc     1080

agccgccccc gcgcggagat ggacgtccgc aacgcgatcg tcctcttctc gctcagcgct     1140

gccggggtca ctcaggagct ggcgatctcg cgccgccagc gccgcgttcc cggcaatctc     1200

atgggctcgt accgatcggt tggcgtcgag acgggtgaga cgaagaagga gggtgctgcc     1260

cgctcgggtg gccagggtgg catccgcgcc cgcctgcggc ccatcgacgc ggacacgatc     1320

gacatctacg cccgcgctgt gttccctgct gccttcgctg ctgtgaacgt catctactgg     1380

gctgcctacg cgtag                                                      1395


<210>  98
<211>  1395
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GABRD GS_BS_GCU ORF

<400>  98
atgagcgaag ccactcccct cgaccgcaac gacagcgaga acacgggtgg cctcatcagc       60

cgcccccacc cctgggacca gagcccctca tgcgtccagg aggaccgcgc gatgaacgac      120

atcggcgact acgttggcag caatctcgag atcagctggc ttccaaatct cgacggcctc      180

atcgccgggt acgcccgcaa cttccgccct ggcatcggtg gcccacccgt caacgtcgca      240

ctggcactgg aagtcgcgag catcgaccac atcagcgaag ccaacatgga gtacacgatg      300

acggtcttcc tgcaccagag ctggcgagac agccgcctca gctacaacca cacgaacgag      360

acgctcggcc tcgacagccg cttcgtcgac aagctctggc ttcctgacac gttcatcgtc      420

aacgcgaaga gcgcgtggtt ccacgacgtc acggtcgaga acaagctcat ccgcctccag      480

cctgacggcg tcatcctcta cagcatccgc atcacgtcga ctgtcgcgtg cgacatggac      540

ctcgcgaagt accccatgga cgagcaggag tgcatgctcg acctcgagag ctacggctac      600

agcagcgagg acatcgtcta ctactggagc gagagccagg agcacatcca cggcctcgac      660

aagctccagc tcgcgcagtt cacgatcacg agctaccgct tcacgacaga gctgatgaac      720

ttcaagagcg ccgggcagtt cccccgcctc agcctccact tccacctgcg gcgcaaccgc      780

ggcgtctaca tcatccagag ctacatgccc tcagtcctcc tcgtcgcgat gagctgggtc      840

agcttctgga tcagccaggc tgctgttccc gcccgcgtca gcctcggcat cacgacggtc      900

ctcacgatga cgacgctcat ggtcagcgcc cgcagcagcc ttccacgcgc gagcgcgatc      960

aaggcactgg acgtctactt ctggatctgc tacgtcttcg tcttcgctgc actggtcgag     1020

tacgcgttcg cgcacttcaa cgcggactac cgcaagaagc agaaggcgaa ggtcaaggtc     1080

agccgccccc gcgcggagat ggacgtccgc aacgcgatcg tcctcttcag cctcagcgct     1140

gccggggtca ctcaggagct ggcgatcagc cgccgccagc gccgcgttcc cggcaatctc     1200

atgggcagct acaggagcgt tggcgtcgag acgggtgaga cgaagaagga gggtgctgcc     1260

cgcagcggtg gccagggtgg catccgcgcc cgcctgcggc ccatcgacgc ggacacgatc     1320

gacatctacg cccgcgctgt gttccctgct gccttcgctg ctgtgaacgt catctactgg     1380

gctgcctacg cgtag                                                      1395


<210>  99
<211>  1395
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GABRD GS_GCU ORF

<400>  99
atgagcgaag ccactcccct ggaccgcaac gacagcgaga acacgggtgg cctgatcagc       60

cgcccgcacc cgtgggacca gagcccctca tgcgtgcagg aggaccgcgc gatgaacgac      120

atcggcgact acgttggcag caatctcgag atcagctggc ttccaaatct cgacggcctg      180

atcgccgggt acgcccgcaa cttccgcccg ggcatcggtg gcccacccgt gaacgtggca      240

ctggcactgg aagtcgcgag catcgaccac atcagcgaag ccaacatgga gtacacgatg      300

acggtgttcc tgcaccagag ctggcgagac agccgcctca gctacaacca cacgaacgag      360

acgctgggcc tggacagccg cttcgtggac aagctgtggc ttcctgacac gttcatcgtg      420

aacgcgaaga gcgcgtggtt ccacgacgtg acggtggaga acaagctgat ccgcctgcag      480

cctgacggcg tgatcctgta cagcatccgc atcacgtcga ctgtggcgtg cgacatggac      540

ctggcgaagt acccgatgga cgagcaggag tgcatgctgg acctggagag ctacggctac      600

agcagcgagg acatcgtgta ctactggagc gagagccagg agcacatcca cggcctggac      660

aagctgcagc tggcgcagtt cacgatcacg agctaccgct tcacgacaga gctgatgaac      720

ttcaagagcg ccgggcagtt cccgcgcctc agcctgcact tccacctgcg gcgcaaccgc      780

ggcgtgtaca tcatccagag ctacatgccc tcagtgctgc tggtggcgat gagctgggtc      840

agcttctgga tcagccaggc tgctgttccc gcccgcgtca gcctgggcat cacgacggtg      900

ctgacgatga cgacgctgat ggtcagcgcc cgcagcagcc ttccacgcgc gagcgcgatc      960

aaggcactgg acgtgtactt ctggatctgc tacgtgttcg tgttcgctgc actggtggag     1020

tacgcgttcg cgcacttcaa cgcggactac cgcaagaagc agaaggcgaa ggtgaaggtc     1080

agccgcccgc gcgcggagat ggacgtgcgc aacgcgatcg tgctgttcag cctcagcgct     1140

gccggggtga ctcaggagct ggcgatcagc cgccgccagc gccgcgttcc cggcaatctc     1200

atgggcagct accgcagcgt tggcgtggag acgggtgaga cgaagaagga gggtgctgcc     1260

cgcagcggtg gccagggtgg catccgcgcc cgcctgcggc cgatcgacgc ggacacgatc     1320

gacatctacg cccgcgctgt gttcccggct gccttcgctg ctgtgaacgt gatctactgg     1380

gctgcctacg cgtag                                                      1395


<210>  100
<211>  316
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: GAPDH amino acid sequence

<400>  100

Met Gly Lys Val Lys Val Gly Val Asn Gly Phe Gly Arg Ile Gly Arg 
1               5                   10                  15      


Leu Val Thr Arg Ala Ala Phe Asn Ser Gly Lys Val Asp Ile Val Ala 
            20                  25                  30          


Ile Asn Asp Pro Phe Ile Asp Leu Asn Tyr Met Ala Glu Asn Gly Lys 
        35                  40                  45              


Leu Val Ile Asn Gly Asn Pro Ile Thr Ile Phe Gln Glu Arg Asp Pro 
    50                  55                  60                  


Ser Lys Ile Lys Trp Gly Asp Ala Gly Ala Glu Tyr Val Val Glu Ser 
65                  70                  75                  80  


Thr Gly Val Phe Thr Thr Met Glu Lys Ala Gly Ala His Leu Gln Gly 
                85                  90                  95      


Gly Ala Lys Arg Val Ile Ile Ser Ala Pro Ser Ala Asp Ala Pro Met 
            100                 105                 110         


Phe Val Met Gly Val Asn His Glu Lys Tyr Asp Asn Ser Leu Lys Ile 
        115                 120                 125             


Ile Ser Asn Ala Ser Cys Thr Thr Asn Cys Leu Ala Pro Leu Ala Lys 
    130                 135                 140                 


Val Ile His Asp Asn Phe Gly Ile Val Glu Gly Leu Met Thr Thr Val 
145                 150                 155                 160 


His Ala Ile Thr Ala Thr Gln Lys Thr Val Asp Gly Pro Ser Gly Lys 
                165                 170                 175     


Leu Trp Arg Asp Gly Arg Gly Ala Leu Gln Asn Ile Ile Pro Ala Ser 
            180                 185                 190         


Thr Gly Ala Ala Lys Ala Val Gly Lys Val Ile Pro Glu Leu Asn Gly 
        195                 200                 205             


Lys Leu Thr Gly Met Ala Phe Arg Val Pro Thr Ala Asn Val Ser Val 
    210                 215                 220                 


Val Asp Leu Thr Cys Arg Leu Glu Lys Pro Ala Lys Tyr Asp Asp Ile 
225                 230                 235                 240 


Lys Lys Val Val Lys Gln Ala Ser Glu Gly Pro Leu Lys Gly Ile Leu 
                245                 250                 255     


Gly Tyr Thr Glu His Gln Val Val Ser Ser Asp Phe Asn Ser Asp Thr 
            260                 265                 270         


His Ser Ser Thr Phe Asp Ala Gly Ala Gly Ile Ala Leu Asn Asp His 
        275                 280                 285             


Phe Val Lys Leu Ile Ser Trp Tyr Asp Asn Glu Phe Gly Tyr Ser Asn 
    290                 295                 300                 


Arg Val Val Asp Leu Met Ala His Met Ala Ser Lys 
305                 310                 315     


<210>  101
<211>  951
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GAPDH WT ORF

<400>  101
auggguaaag uuaaaguugg uguuaauggu uuuggucgua uuggucguuu aguuacucgu       60

gcugcuuuua auucugguaa aguugauauu guugcuauua augauccuuu uauugauuua      120

aauuauaugg cugaaaaugg uaaauuaguu auuaauggua auccuauuac uauuuuucaa      180

gaacgugauc cuucuaaaau uaaauggggu gaugcuggug cugaauaugu uguugaaucu      240

acugguguuu uuacuacuau ggaaaaagcu ggugcucauu uacaaggugg ugcuaaacgu      300

guuauuauuu cugcuccuuc ugcugaugcu ccuauguuug uuaugggugu uaaucaugaa      360

aaauaugaua auucuuuaaa aauuauuucu aaugcuucuu guacuacuaa uuguuuagcu      420

ccuuuagcua aaguuauuca ugauaauuuu gguauuguug aagguuuaau gacuacuguu      480

caugcuauua cugcuacuca aaaaacuguu gaugguccuu cugguaaauu auggcgugau      540

ggucguggug cuuuacaaaa uauuauuccu gcuucuacug gugcugcuaa agcuguuggu      600

aaaguuauuc cugaauuaaa ugguaaauua acugguaugg cuuuucgugu uccuacugcu      660

aauguuucug uuguugauuu aacuugucgu uuagaaaaac cugcuaaaua ugaugauauu      720

aaaaaaguug uuaaacaagc uucugaaggu ccuuuaaaag guauuuuagg uuauacugaa      780

caucaaguug uuucuucuga uuuuaauucu gauacucauu cuucuacuuu ugaugcuggu      840

gcugguauug cuuuaaauga ucauuuuguu aaauuaauuu cuugguauga uaaugaauuu      900

gguuauucua aucguguugu ugauuuaaug gcucauaugg cuucuaaaua g               951


<210>  102
<211>  951
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GAPDH BP_GCU ORF

<400>  102
atgggcaagg tcaaggtcgg cgtcaacggc ttcggccgca tcggccgcct cgtcacgcgc       60

gcggcgttca actcgggcaa ggtcgacatc gtcgcgatca acgacccctt catcgacctc      120

aactacatgg cggagaacgg caagctcgtc atcaacggca accccatcac gatcttccag      180

gagcgcgacc cctcgaagat caagtggggc gacgcgggcg cggagtacgt cgtcgagtcg      240

acgggcgtct tcacgacgat ggagaaggcg ggcgcgcacc tccagggcgg cgcgaagcgc      300

gtcatcatct cggcgccctc ggcggacgcg cccatgttcg tcatgggcgt caaccacgag      360

aagtacgaca actcgctcaa gatcatctcg aacgcgtcgt gcacgacgaa ctgcctcgcg      420

cccctcgcga aggtcatcca cgacaacttc ggcatcgtcg agggcctcat gacgacggtc      480

cacgcgatca cggcgacgca gaagacggtc gacggcccct cgggcaagct ctggcgcgac      540

ggccgcggcg cgctccagaa catcatcccc gcgtcgacgg gcgcggcgaa ggcggtcggc      600

aaggtcatcc ccgagctcaa cggcaagctc acgggcatgg cgttccgcgt ccccacggcg      660

aacgtctcgg tcgtcgacct cacgtgccgc ctcgagaagc ccgcgaagta cgacgacatc      720

aagaaggtcg tcaagcaggc gtcggagggc cccctcaagg gcatcctcgg ctacacggag      780

caccaggtcg tctcgtcgga cttcaactcg gacacgcact cgtcgacgtt cgacgcgggc      840

gcgggcatcg cgctcaacga ccacttcgtc aagctcatct cgtggtacga caacgagttc      900

ggctactcga accgcgtcgt cgacctcatg gcgcacatgg cgtcgaagta g               951


<210>  103
<211>  951
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GAPDH GP_BP_BS_GCU ORF

<400>  103
atgggcaagg tcaaggttgg cgtcaatggg ttcggccgca tcggccgcct cgtcacgcgc       60

gctgccttca actcgggcaa ggtcgacatc gtcgcgatca acgacccctt catcgacctc      120

aactacatgg cggagaatgg gaagctcgtc atcaatggga accccatcac gatcttccag      180

gagcgagacc cctcaaagat caagtggggc gacgccggtg ccgagtacgt cgtcgagtcg      240

actggcgtct tcacgacgat ggagaaggcc ggtgcccacc tccagggtgg tgccaagcgc      300

gtcatcatct cggcgccctc agcggacgcg cccatgttcg tcatgggcgt caaccacgag      360

aagtacgaca actcgctcaa gatcatctcg aacgcgtcgt gcacgacgaa ctgcctcgcg      420

cccctcgcga aggtcatcca cgacaacttc ggcatcgtcg agggcctcat gacgacggtc      480

cacgcgatca cggcgactca aaagacggtc gacggcccct caggcaagct ctggcgagac      540

ggccgcggtg cactgcagaa catcatcccc gcgtcgactg gtgctgccaa ggctgttggc      600

aaggtcatcc cagagctgaa tgggaagctc acgggcatgg cgttccgcgt tcccaccgcg      660

aacgtcagcg tcgtcgacct cacgtgccgc ctcgagaagc ctgcgaagta cgacgacatc      720

aagaaggtcg tcaagcaggc ctcggagggc cccctcaagg ggatcctcgg ctacacagag      780

caccaggtgg tcagctcgga cttcaactcg gacacgcact cgtcgacttt cgacgccggt      840

gccgggatcg cactgaacga ccacttcgtc aagctcatct cgtggtacga caacgagttc      900

ggctactcga accgcgtcgt cgacctcatg gcgcacatgg cgtcgaagta g               951


<210>  104
<211>  951
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GAPDH GS_BS_GCU ORF

<400>  104
atgggcaagg tcaaggttgg cgtcaatggg ttcggccgca tcggccgcct cgtcacgcgc       60

gctgccttca acagcggcaa ggtcgacatc gtcgcgatca acgacccctt catcgacctc      120

aactacatgg cggagaatgg gaagctcgtc atcaatggga accccatcac gatcttccag      180

gagcgagacc cctcaaagat caagtggggc gacgccggtg ccgagtacgt cgtcgagtcg      240

actggcgtct tcacgacgat ggagaaggcc ggtgcccacc tccagggtgg tgccaagcgc      300

gtcatcatca gcgcgccctc agcggacgcg cccatgttcg tcatgggcgt caaccacgag      360

aagtacgaca acagcctcaa gatcatcagc aacgcgagct gcacgacgaa ctgcctcgcg      420

cccctcgcga aggtcatcca cgacaacttc ggcatcgtcg agggcctcat gacgacggtc      480

cacgcgatca cggcgactca aaagacggtc gacggcccct caggcaagct ctggcgagac      540

ggccgcggtg cactgcagaa catcatcccc gcgtcgactg gtgctgccaa ggctgttggc      600

aaggtcatcc cagagctgaa tgggaagctc acgggcatgg cgttccgcgt tcccaccgcg      660

aacgtcagcg tcgtcgacct cacgtgccgc ctcgagaagc ctgcgaagta cgacgacatc      720

aagaaggtcg tcaagcaggc cagcgagggc cccctcaagg ggatcctcgg ctacacagag      780

caccaggtgg tcagcagcga cttcaacagc gacacgcaca gctcgacttt cgacgccggt      840

gccgggatcg cactgaacga ccacttcgtc aagctcatca gctggtacga caacgagttc      900

ggctacagca accgcgtcgt cgacctcatg gcgcacatgg cgagcaagta g               951


<210>  105
<211>  951
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GAPDH GS_GCU ORF

<400>  105
atgggcaagg tgaaggttgg cgtgaatggg ttcggccgca tcggccgcct ggtgacgcgc       60

gctgccttca acagcggcaa ggtggacatc gtggcgatca acgacccgtt catcgacctg      120

aactacatgg cggagaatgg gaagctggtg atcaatggga acccgatcac gatcttccag      180

gagcgagacc cctcaaagat caagtggggc gacgccggtg ccgagtacgt ggtggagtcg      240

actggcgtgt tcacgacgat ggagaaggcc ggtgcccacc tgcagggtgg tgccaagcgc      300

gtgatcatca gcgcgccctc agcggacgcg ccgatgttcg tgatgggcgt gaaccacgag      360

aagtacgaca acagcctgaa gatcatcagc aacgcgagct gcacgacgaa ctgcctggcg      420

ccgctggcga aggtgatcca cgacaacttc ggcatcgtgg agggcctgat gacgacggtg      480

cacgcgatca cggcgactca aaagacggtg gacggcccct caggcaagct gtggcgagac      540

ggccgcggtg cactgcagaa catcatcccc gcgtcgactg gtgctgccaa ggctgttggc      600

aaggtgatcc cagagctgaa tgggaagctg acgggcatgg cgttccgcgt tcccaccgcg      660

aacgtcagcg tggtggacct gacgtgccgc ctggagaagc cggcgaagta cgacgacatc      720

aagaaggtgg tgaagcaggc cagcgagggc ccgctgaagg ggatcctggg ctacacagag      780

caccaggtgg tcagcagcga cttcaacagc gacacgcaca gctcgacttt cgacgccggt      840

gccgggatcg cactgaacga ccacttcgtg aagctgatca gctggtacga caacgagttc      900

ggctacagca accgcgtggt ggacctgatg gcgcacatgg cgagcaagta g               951


<210>  106
<211>  535
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: GBA1 amino acid sequence

<400>  106

Met Glu Phe Ser Ser Pro Ser Arg Glu Glu Cys Pro Lys Pro Leu Ser 
1               5                   10                  15      


Arg Val Ser Ile Met Ala Gly Ser Leu Thr Gly Leu Leu Leu Leu Gln 
            20                  25                  30          


Ala Val Ser Trp Ala Ser Gly Ala Arg Pro Cys Ile Pro Lys Ser Phe 
        35                  40                  45              


Gly Tyr Ser Ser Val Val Cys Val Cys Asn Ala Thr Tyr Cys Asp Ser 
    50                  55                  60                  


Phe Asp Pro Pro Thr Phe Pro Ala Leu Gly Thr Phe Ser Arg Tyr Glu 
65                  70                  75                  80  


Ser Thr Arg Ser Gly Arg Arg Met Glu Leu Ser Met Gly Pro Ile Gln 
                85                  90                  95      


Ala Asn His Thr Gly Thr Gly Leu Leu Leu Thr Leu Gln Pro Glu Gln 
            100                 105                 110         


Lys Phe Gln Lys Val Lys Gly Phe Gly Gly Ala Met Thr Asp Ala Ala 
        115                 120                 125             


Ala Leu Asn Ile Leu Ala Leu Ser Pro Pro Ala Gln Asn Leu Leu Leu 
    130                 135                 140                 


Lys Ser Tyr Phe Ser Glu Glu Gly Ile Gly Tyr Asn Ile Ile Arg Val 
145                 150                 155                 160 


Pro Met Ala Ser Cys Asp Phe Ser Ile Arg Thr Tyr Thr Tyr Ala Asp 
                165                 170                 175     


Thr Pro Asp Asp Phe Gln Leu His Asn Phe Ser Leu Pro Glu Glu Asp 
            180                 185                 190         


Thr Lys Leu Lys Ile Pro Leu Ile His Arg Ala Leu Gln Leu Ala Gln 
        195                 200                 205             


Arg Pro Val Ser Leu Leu Ala Ser Pro Trp Thr Ser Pro Thr Trp Leu 
    210                 215                 220                 


Lys Thr Asn Gly Ala Val Asn Gly Lys Gly Ser Leu Lys Gly Gln Pro 
225                 230                 235                 240 


Gly Asp Ile Tyr His Gln Thr Trp Ala Arg Tyr Phe Val Lys Phe Leu 
                245                 250                 255     


Asp Ala Tyr Ala Glu His Lys Leu Gln Phe Trp Ala Val Thr Ala Glu 
            260                 265                 270         


Asn Glu Pro Ser Ala Gly Leu Leu Ser Gly Tyr Pro Phe Gln Cys Leu 
        275                 280                 285             


Gly Phe Thr Pro Glu His Gln Arg Asp Phe Ile Ala Arg Asp Leu Gly 
    290                 295                 300                 


Pro Thr Leu Ala Asn Ser Thr His His Asn Val Arg Leu Leu Met Leu 
305                 310                 315                 320 


Asp Asp Gln Arg Leu Leu Leu Pro His Trp Ala Lys Val Val Leu Thr 
                325                 330                 335     


Asp Pro Glu Ala Ala Lys Tyr Val His Gly Ile Ala Val His Trp Tyr 
            340                 345                 350         


Leu Asp Phe Leu Ala Pro Ala Lys Ala Thr Leu Gly Glu Thr His Arg 
        355                 360                 365             


Leu Phe Pro Asn Thr Met Leu Phe Ala Ser Glu Ala Cys Val Gly Ser 
    370                 375                 380                 


Lys Phe Trp Glu Gln Ser Val Arg Leu Gly Ser Trp Asp Arg Gly Met 
385                 390                 395                 400 


Gln Tyr Ser His Ser Ile Ile Thr Asn Leu Leu Tyr His Val Val Gly 
                405                 410                 415     


Trp Thr Asp Trp Asn Leu Ala Leu Asn Pro Glu Gly Gly Pro Asn Trp 
            420                 425                 430         


Val Arg Asn Phe Val Asp Ser Pro Ile Ile Val Asp Ile Thr Lys Asp 
        435                 440                 445             


Thr Phe Tyr Lys Gln Pro Met Phe Tyr His Leu Gly His Phe Ser Lys 
    450                 455                 460                 


Phe Ile Pro Glu Gly Ser Gln Arg Val Gly Leu Val Ala Ser Gln Lys 
465                 470                 475                 480 


Asn Asp Leu Asp Ala Val Ala Leu Met His Pro Asp Gly Ser Ala Val 
                485                 490                 495     


Val Val Val Leu Asn Arg Ser Ser Lys Asp Val Pro Leu Thr Ile Lys 
            500                 505                 510         


Asp Pro Ala Val Gly Phe Leu Glu Thr Ile Ser Pro Gly Tyr Ser Ile 
        515                 520                 525             


His Thr Tyr Leu Trp Arg Arg 
    530                 535 


<210>  107
<211>  1608
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GBA1 WT ORF

<400>  107
auggaauuuu cuucuccuuc ucgugaagaa uguccuaaac cuuuaucucg uguuucuauu       60

auggcugguu cuuuaacugg uuuauuauua uuacaagcug uuucuugggc uucuggugcu      120

cguccuugua uuccuaaauc uuuugguuau ucuucuguug uuuguguuug uaaugcuacu      180

uauugugauu cuuuugaucc uccuacuuuu ccugcuuuag guacuuuuuc ucguuaugaa      240

ucuacucguu cuggucgucg uauggaauua ucuauggguc cuauucaagc uaaucauacu      300

gguacugguu uauuauuaac uuuacaaccu gaacaaaaau uucaaaaagu uaaagguuuu      360

gguggugcua ugacugaugc ugcugcuuua aauauuuuag cuuuaucucc uccugcucaa      420

aauuuauuau uaaaaucuua uuuuucugaa gaagguauug guuauaauau uauucguguu      480

ccuauggcuu cuugugauuu uucuauucgu acuuauacuu augcugauac uccugaugau      540

uuucaauuac auaauuuuuc uuuaccugaa gaagauacua aauuaaaaau uccuuuaauu      600

caucgugcuu uacaauuagc ucaacguccu guuucuuuau uagcuucucc uuggacuucu      660

ccuacuuggu uaaaaacuaa uggugcuguu aaugguaaag guucuuuaaa aggucaaccu      720

ggugauauuu aucaucaaac uugggcucgu uauuuuguua aauuuuuaga ugcuuaugcu      780

gaacauaaau uacaauuuug ggcuguuacu gcugaaaaug aaccuucugc ugguuuauua      840

ucugguuauc cuuuucaaug uuuagguuuu acuccugaac aucaacguga uuuuauugcu      900

cgugauuuag guccuacuuu agcuaauucu acucaucaua auguucguuu auuaauguua      960

gaugaucaac guuuauuauu accucauugg gcuaaaguug uuuuaacuga uccugaagcu     1020

gcuaaauaug uucaugguau ugcuguucau ugguauuuag auuuuuuagc uccugcuaaa     1080

gcuacuuuag gugaaacuca ucguuuauuu ccuaauacua uguuauuugc uucugaagcu     1140

uguguugguu cuaaauuuug ggaacaaucu guucguuuag guucuuggga ucgugguaug     1200

caauauucuc auucuauuau uacuaauuua uuauaucaug uuguugguug gacugauugg     1260

aauuuagcuu uaaauccuga aggugguccu aauuggguuc guaauuuugu ugauucuccu     1320

auuauuguug auauuacuaa agauacuuuu uauaaacaac cuauguuuua ucauuuaggu     1380

cauuuuucua aauuuauucc ugaagguucu caacguguug guuuaguugc uucucaaaaa     1440

aaugauuuag augcuguugc uuuaaugcau ccugaugguu cugcuguugu uguuguuuua     1500

aaucguucuu cuaaagaugu uccuuuaacu auuaaagauc cugcuguugg uuuuuuagaa     1560

acuauuucuc cugguuauuc uauucauacu uauuuauggc gucguuag                  1608


<210>  108
<211>  1608
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GBA1 BP_GCU ORF

<400>  108
atggagttct cgtcgccctc gcgcgaggag tgccccaagc ccctctcgcg cgtctcgatc       60

atggcgggct cgctcacggg cctcctcctc ctccaggcgg tctcgtgggc gtcgggcgcg      120

cgcccctgca tccccaagtc gttcggctac tcgtcggtcg tctgcgtctg caacgcgacg      180

tactgcgact cgttcgaccc ccccacgttc cccgcgctcg gcacgttctc gcgctacgag      240

tcgacgcgct cgggccgccg catggagctc tcgatgggcc ccatccaggc gaaccacacg      300

ggcacgggcc tcctcctcac gctccagccc gagcagaagt tccagaaggt caagggcttc      360

ggcggcgcga tgacggacgc ggcggcgctc aacatcctcg cgctctcgcc ccccgcgcag      420

aacctcctcc tcaagtcgta cttctcggag gagggcatcg gctacaacat catccgcgtc      480

cccatggcgt cgtgcgactt ctcgatccgc acgtacacgt acgcggacac gcccgacgac      540

ttccagctcc acaacttctc gctccccgag gaggacacga agctcaagat ccccctcatc      600

caccgcgcgc tccagctcgc gcagcgcccc gtctcgctcc tcgcgtcgcc ctggacgtcg      660

cccacgtggc tcaagacgaa cggcgcggtc aacggcaagg gctcgctcaa gggccagccc      720

ggcgacatct accaccagac gtgggcgcgc tacttcgtca agttcctcga cgcgtacgcg      780

gagcacaagc tccagttctg ggcggtcacg gcggagaacg agccctcggc gggcctcctc      840

tcgggctacc ccttccagtg cctcggcttc acgcccgagc accagcgcga cttcatcgcg      900

cgcgacctcg gccccacgct cgcgaactcg acgcaccaca acgtccgcct cctcatgctc      960

gacgaccagc gcctcctcct cccccactgg gcgaaggtcg tcctcacgga ccccgaggcg     1020

gcgaagtacg tccacggcat cgcggtccac tggtacctcg acttcctcgc gcccgcgaag     1080

gcgacgctcg gcgagacgca ccgcctcttc cccaacacga tgctcttcgc gtcggaggcg     1140

tgcgtcggct cgaagttctg ggagcagtcg gtccgcctcg gctcgtggga ccgcggcatg     1200

cagtactcgc actcgatcat cacgaacctc ctctaccacg tcgtcggctg gacggactgg     1260

aacctcgcgc tcaaccccga gggcggcccc aactgggtcc gcaacttcgt cgactcgccc     1320

atcatcgtcg acatcacgaa ggacacgttc tacaagcagc ccatgttcta ccacctcggc     1380

cacttctcga agttcatccc cgagggctcg cagcgcgtcg gcctcgtcgc gtcgcagaag     1440

aacgacctcg acgcggtcgc gctcatgcac cccgacggct cggcggtcgt cgtcgtcctc     1500

aaccgctcgt cgaaggacgt ccccctcacg atcaaggacc ccgcggtcgg cttcctcgag     1560

acgatctcgc ccggctactc gatccacacg tacctctggc gccgctag                  1608


<210>  109
<211>  1608
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GBA1 GP_BP_BS_GCU ORF

<400>  109
atggagttct cgtcgccctc aagggaggag tgccccaagc ccctcagccg cgtcagcatc       60

atggccgggt cgctcacggg cctcctcctc ctccaggctg tcagctgggc gtcgggtgcc      120

cgcccctgca tccccaagtc gttcggctac tcgtcggtcg tctgcgtctg caacgcgacc      180

tactgcgact cgttcgaccc acccaccttc cctgcactgg gcacgttctc gcgctacgag      240

tcgactcgat cgggccgccg catggagctc agcatgggcc ccatccaggc caaccacacg      300

ggcacgggcc tcctcctcac gctccagcca gagcagaagt tccagaaggt caaggggttc      360

ggtggtgcca tgacggacgc tgctgcactg aacatcctcg cactcagccc acccgcgcag      420

aatctcctcc tcaagtcgta cttctcggag gagggcatcg gctacaacat catccgcgtt      480

cccatggcgt cgtgcgactt cagcatccgc acctacacct acgcggacac tcctgacgac      540

ttccagctcc acaacttctc gcttccagag gaggacacga agctcaagat ccccctcatc      600

caccgcgcac tgcagctcgc gcagcgcccc gtcagcctcc tcgcgtcgcc ctggacgtcg      660

cccacctggc tcaagacgaa tggtgctgtg aatgggaagg ggtcgctcaa ggggcagccc      720

ggcgacatct accaccagac gtgggcccgc tacttcgtca agttcctgga cgcgtacgcg      780

gagcacaagc tccagttctg ggctgtgacg gcggagaacg agccctcagc cgggctcctc      840

agcggctacc ccttccagtg cctcggcttc actccagagc accagcgaga cttcatcgcc      900

cgagacctcg gccccaccct cgcgaactcg actcaccaca acgtccgcct cctcatgctc      960

gacgaccagc gcctcctcct tccacactgg gcgaaggtcg tcctcacgga cccagaagct     1020

gccaagtacg tccacggcat cgctgtgcac tggtacctcg acttcctggc gcctgcgaag     1080

gcgacgctcg gtgagacgca ccgcctcttc cccaacacga tgctcttcgc gtcggaagcc     1140

tgcgttggct cgaagttctg ggagcagagc gtccgcctcg gctcgtggga ccgcggcatg     1200

cagtactcgc acagcatcat cacgaatctc ctctaccacg tcgttggctg gacggactgg     1260

aatctcgcac tgaacccaga gggtggcccc aactgggtcc gcaacttcgt cgactcgccc     1320

atcatcgtcg acatcacgaa ggacacgttc tacaagcagc ccatgttcta ccacctcggc     1380

cacttctcga agttcatccc agagggctcg cagcgcgttg gcctcgtcgc gtcgcagaag     1440

aacgacctcg acgctgtggc actgatgcac cctgacggct cggctgtggt cgtcgtcctc     1500

aaccgatcgt cgaaggacgt tcccctcacg atcaaggacc ctgctgttgg cttcctggag     1560

acgatctcgc ctggctacag catccacacc tacctctggc gccgctag                  1608


<210>  110
<211>  1608
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GBA1 GS_BS_GCU ORF

<400>  110
atggagttca gcagcccctc aagggaggag tgccccaagc ccctcagccg cgtcagcatc       60

atggccggga gcctcacggg cctcctcctc ctccaggctg tcagctgggc gagcggtgcc      120

cgcccctgca tccccaagag cttcggctac agcagcgtcg tctgcgtctg caacgcgacc      180

tactgcgaca gcttcgaccc acccaccttc cctgcactgg gcacgttcag ccgctacgag      240

tcgactagga gcggccgccg catggagctc agcatgggcc ccatccaggc caaccacacg      300

ggcacgggcc tcctcctcac gctccagcca gagcagaagt tccagaaggt caaggggttc      360

ggtggtgcca tgacggacgc tgctgcactg aacatcctcg cactcagccc acccgcgcag      420

aatctcctcc tcaagagcta cttcagcgag gagggcatcg gctacaacat catccgcgtt      480

cccatggcga gctgcgactt cagcatccgc acctacacct acgcggacac tcctgacgac      540

ttccagctcc acaacttcag ccttccagag gaggacacga agctcaagat ccccctcatc      600

caccgcgcac tgcagctcgc gcagcgcccc gtcagcctcc tcgcgagccc ctggacgagc      660

cccacctggc tcaagacgaa tggtgctgtg aatgggaagg ggagcctcaa ggggcagccc      720

ggcgacatct accaccagac gtgggcccgc tacttcgtca agttcctgga cgcgtacgcg      780

gagcacaagc tccagttctg ggctgtgacg gcggagaacg agccctcagc cgggctcctc      840

agcggctacc ccttccagtg cctcggcttc actccagagc accagcgaga cttcatcgcc      900

cgagacctcg gccccaccct cgcgaactcg actcaccaca acgtccgcct cctcatgctc      960

gacgaccagc gcctcctcct tccacactgg gcgaaggtcg tcctcacgga cccagaagct     1020

gccaagtacg tccacggcat cgctgtgcac tggtacctcg acttcctggc gcctgcgaag     1080

gcgacgctcg gtgagacgca ccgcctcttc cccaacacga tgctcttcgc gagcgaagcc     1140

tgcgttggca gcaagttctg ggagcagagc gtccgcctcg gcagctggga ccgcggcatg     1200

cagtacagcc acagcatcat cacgaatctc ctctaccacg tcgttggctg gacggactgg     1260

aatctcgcac tgaacccaga gggtggcccc aactgggtcc gcaacttcgt cgacagcccc     1320

atcatcgtcg acatcacgaa ggacacgttc tacaagcagc ccatgttcta ccacctcggc     1380

cacttcagca agttcatccc agagggcagc cagcgcgttg gcctcgtcgc gagccagaag     1440

aacgacctcg acgctgtggc actgatgcac cctgacggca gcgctgtggt cgtcgtcctc     1500

aacaggagca gcaaggacgt tcccctcacg atcaaggacc ctgctgttgg cttcctggag     1560

acgatcagcc ctggctacag catccacacc tacctctggc gccgctag                  1608


<210>  111
<211>  1608
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GBA1 GS_GCU ORF

<400>  111
atggagttca gcagcccctc aagggaggag tgcccgaagc cgctcagccg cgtcagcatc       60

atggccggga gcctgacggg cctgctgctg ctgcaggctg tcagctgggc gagcggtgcc      120

cgcccgtgca tccccaagag cttcggctac agcagcgtgg tgtgcgtgtg caacgcgacc      180

tactgcgaca gcttcgaccc acccaccttc ccggcactgg gcacgttcag ccgctacgag      240

tcgactcgca gcggccgccg catggagctc agcatgggcc cgatccaggc caaccacacg      300

ggcacgggcc tgctgctgac gctgcagcca gagcagaagt tccagaaggt gaaggggttc      360

ggtggtgcca tgacggacgc tgctgcactg aacatcctgg cactcagccc acccgcgcag      420

aatctcctgc tgaagagcta cttcagcgag gagggcatcg gctacaacat catccgcgtt      480

cccatggcga gctgcgactt cagcatccgc acctacacct acgcggacac tcctgacgac      540

ttccagctgc acaacttcag ccttccagag gaggacacga agctgaagat ccccctgatc      600

caccgcgcac tgcagctggc gcagcgcccg gtcagcctgc tggcgagccc gtggacgagc      660

cccacctggc tgaagacgaa tggtgctgtg aatgggaagg ggagcctgaa ggggcagccc      720

ggcgacatct accaccagac gtgggcccgc tacttcgtga agttcctgga cgcgtacgcg      780

gagcacaagc tgcagttctg ggctgtgacg gcggagaacg agccctcagc cgggctgctc      840

agcggctacc cgttccagtg cctgggcttc actccagagc accagcgaga cttcatcgcc      900

cgagacctgg gccccaccct ggcgaactcg actcaccaca acgtgcgcct gctgatgctg      960

gacgaccagc gcctgctgct tccacactgg gcgaaggtgg tgctgacgga cccagaagct     1020

gccaagtacg tgcacggcat cgctgtgcac tggtacctgg acttcctggc gccggcgaag     1080

gcgacgctgg gtgagacgca ccgcctgttc ccgaacacga tgctgttcgc gagcgaagcc     1140

tgcgttggca gcaagttctg ggagcagagc gtgcgcctgg gcagctggga ccgcggcatg     1200

cagtacagcc acagcatcat cacgaatctc ctgtaccacg tggttggctg gacggactgg     1260

aatctcgcac tgaacccaga gggtggcccg aactgggtgc gcaacttcgt ggacagcccg     1320

atcatcgtgg acatcacgaa ggacacgttc tacaagcagc ccatgttcta ccacctgggc     1380

cacttcagca agttcatccc agagggcagc cagcgcgttg gcctggtggc gagccagaag     1440

aacgacctgg acgctgtggc actgatgcac cctgacggca gcgctgtggt ggtggtgctg     1500

aaccgcagca gcaaggacgt tcccctgacg atcaaggacc cggctgttgg cttcctggag     1560

acgatcagcc cgggctacag catccacacc tacctgtggc gccgctag                  1608


<210>  112
<211>  428
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: GLA amino acid sequence

<400>  112

Met Gln Leu Arg Asn Pro Glu Leu His Leu Gly Cys Ala Leu Ala Leu 
1               5                   10                  15      


Arg Phe Leu Ala Leu Val Ser Trp Asp Ile Pro Gly Ala Arg Ala Leu 
            20                  25                  30          


Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp Glu 
        35                  40                  45              


Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys Ile 
    50                  55                  60                  


Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu Gly 
65                  70                  75                  80  


Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp Met 
                85                  90                  95      


Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln Arg 
            100                 105                 110         


Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys Gly 
        115                 120                 125             


Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala Gly 
    130                 135                 140                 


Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe Ala 
145                 150                 155                 160 


Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp Ser 
                165                 170                 175     


Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu Asn 
            180                 185                 190         


Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr Met 
        195                 200                 205             


Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys Asn 
    210                 215                 220                 


His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile Lys 
225                 230                 235                 240 


Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp Val 
                245                 250                 255     


Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly Asn 
            260                 265                 270         


Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp Ala 
        275                 280                 285             


Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile Ser 
    290                 295                 300                 


Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile Asn 
305                 310                 315                 320 


Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp Asn 
                325                 330                 335     


Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val Ala 
            340                 345                 350         


Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile Ala 
        355                 360                 365             


Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe Ile 
    370                 375                 380                 


Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp Thr 
385                 390                 395                 400 


Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu Gln 
                405                 410                 415     


Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
            420                 425             


<210>  113
<211>  1287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GLA WT ORF

<400>  113
augcaauuac guaauccuga auuacauuua gguugugcuu uagcuuuacg uuuuuuagcu       60

uuaguuucuu gggauauucc uggugcucgu gcuuuagaua augguuuagc ucguacuccu      120

acuauggguu gguuacauug ggaacguuuu auguguaauu uagauuguca agaagaaccu      180

gauucuugua uuucugaaaa auuauuuaug gaaauggcug aauuaauggu uucugaaggu      240

uggaaagaug cugguuauga auauuuaugu auugaugauu guuggauggc uccucaacgu      300

gauucugaag gucguuuaca agcugauccu caacguuuuc cucaugguau ucgucaauua      360

gcuaauuaug uucauucuaa agguuuaaaa uuagguauuu augcugaugu ugguaauaaa      420

acuugugcug guuuuccugg uucuuuuggu uauuaugaua uugaugcuca aacuuuugcu      480

gauuggggug uugauuuauu aaaauuugau gguuguuauu gugauucuuu agaaaauuua      540

gcugaugguu auaaacauau gucuuuagcu uuaaaucgua cuggucguuc uauuguuuau      600

ucuugugaau ggccuuuaua uauguggccu uuucaaaaac cuaauuauac ugaaauucgu      660

caauauugua aucauuggcg uaauuuugcu gauauugaug auucuuggaa aucuauuaaa      720

ucuauuuuag auuggacuuc uuuuaaucaa gaacguauug uugauguugc ugguccuggu      780

gguuggaaug auccugauau guuaguuauu gguaauuuug guuuaucuug gaaucaacaa      840

guuacucaaa uggcuuuaug ggcuauuaug gcugcuccuu uauuuauguc uaaugauuua      900

cgucauauuu cuccucaagc uaaagcuuua uuacaagaua aagauguuau ugcuauuaau      960

caagauccuu uagguaaaca agguuaucaa uuacgucaag gugauaauuu ugaaguuugg     1020

gaacguccuu uaucugguuu agcuugggcu guugcuauga uuaaucguca agaaauuggu     1080

gguccucguu cuuauacuau ugcuguugcu ucuuuaggua aagguguugc uuguaauccu     1140

gcuuguuuua uuacucaauu auuaccuguu aaacguaaau uagguuuuua ugaauggacu     1200

ucucguuuac guucucauau uaauccuacu gguacuguuu uauuacaauu agaaaauacu     1260

augcaaaugu cuuuaaaaga uuuauag                                         1287


<210>  114
<211>  1287
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GLA BP_GCU ORF

<400>  114
atgcagctcc gcaaccccga gctccacctc ggctgcgcgc tcgcgctccg cttcctcgcg       60

ctcgtctcgt gggacatccc cggcgcgcgc gcgctcgaca acggcctcgc gcgcacgccc      120

acgatgggct ggctccactg ggagcgcttc atgtgcaacc tcgactgcca ggaggagccc      180

gactcgtgca tctcggagaa gctcttcatg gagatggcgg agctcatggt ctcggagggc      240

tggaaggacg cgggctacga gtacctctgc atcgacgact gctggatggc gccccagcgc      300

gactcggagg gccgcctcca ggcggacccc cagcgcttcc cccacggcat ccgccagctc      360

gcgaactacg tccactcgaa gggcctcaag ctcggcatct acgcggacgt cggcaacaag      420

acgtgcgcgg gcttccccgg ctcgttcggc tactacgaca tcgacgcgca gacgttcgcg      480

gactggggcg tcgacctcct caagttcgac ggctgctact gcgactcgct cgagaacctc      540

gcggacggct acaagcacat gtcgctcgcg ctcaaccgca cgggccgctc gatcgtctac      600

tcgtgcgagt ggcccctcta catgtggccc ttccagaagc ccaactacac ggagatccgc      660

cagtactgca accactggcg caacttcgcg gacatcgacg actcgtggaa gtcgatcaag      720

tcgatcctcg actggacgtc gttcaaccag gagcgcatcg tcgacgtcgc gggccccggc      780

ggctggaacg accccgacat gctcgtcatc ggcaacttcg gcctctcgtg gaaccagcag      840

gtcacgcaga tggcgctctg ggcgatcatg gcggcgcccc tcttcatgtc gaacgacctc      900

cgccacatct cgccccaggc gaaggcgctc ctccaggaca aggacgtcat cgcgatcaac      960

caggaccccc tcggcaagca gggctaccag ctccgccagg gcgacaactt cgaggtctgg     1020

gagcgccccc tctcgggcct cgcgtgggcg gtcgcgatga tcaaccgcca ggagatcggc     1080

ggcccccgct cgtacacgat cgcggtcgcg tcgctcggca agggcgtcgc gtgcaacccc     1140

gcgtgcttca tcacgcagct cctccccgtc aagcgcaagc tcggcttcta cgagtggacg     1200

tcgcgcctcc gctcgcacat caaccccacg ggcacggtcc tcctccagct cgagaacacg     1260

atgcagatgt cgctcaagga cctctag                                         1287


<210>  115
<211>  1287
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GLA GP_BP_BS_GCU ORF

<400>  115
atgcagctgc ggaacccaga gctgcacctc ggctgcgcac tggcactgcg gttcctggca       60

ctggtcagct gggacatccc cggtgcccgc gcactggaca atgggctcgc ccgcactccc      120

accatgggct ggctccactg ggagcgcttc atgtgcaatc tcgactgcca ggaggagcct      180

gactcgtgca tctcggagaa gctcttcatg gagatggcgg agctgatggt cagcgagggc      240

tggaaggacg ccgggtacga gtacctctgc atcgacgact gctggatggc gccccagcga      300

gactcggagg gccgcctcca ggccgacccc cagcgcttcc cccacggcat ccgccagctc      360

gcgaactacg tccactcgaa ggggctcaag ctcggcatct acgcggacgt tggcaacaag      420

acgtgcgccg ggttccctgg ctcgttcggc tactacgaca tcgacgcgca gacgttcgcg      480

gactggggcg tcgacctcct caagttcgac ggctgctact gcgactcgct cgagaatctc      540

gcggacggct acaagcacat gtcgctcgca ctgaaccgca cgggccgaag catcgtctac      600

tcgtgcgagt ggcccctcta catgtggccc ttccagaagc ccaactacac agagatccgc      660

cagtactgca accactggcg caacttcgcg gacatcgacg actcgtggaa gagcatcaag      720

agcatcctcg actggacgtc gttcaaccag gagcgcatcg tcgacgtcgc cgggcctggt      780

ggctggaacg accctgacat gctcgtcatc ggcaacttcg gcctcagctg gaaccagcag      840

gtgactcaaa tggcactgtg ggcgatcatg gctgcccccc tcttcatgtc gaacgacctg      900

cggcacatct cgccccaggc caaggcactg ctccaggaca aggacgtcat cgcgatcaac      960

caggaccccc tcggcaagca gggctaccag ctgcggcagg gcgacaactt cgaagtctgg     1020

gagcgccccc tcagcggcct cgcgtgggct gtggcgatga taaaccgcca ggagatcggt     1080

ggcccccgat cgtacacgat cgctgtggcg tcgctcggca agggggtcgc gtgcaaccct     1140

gcgtgcttca tcactcaact ccttccagtc aagcgcaagc tcggcttcta cgagtggacg     1200

tcgcgcctgc ggtcgcacat caaccccacc ggcacggtcc tcctccagct cgagaacacg     1260

atgcagatgt cgctcaagga cctctag                                         1287


<210>  116
<211>  1287
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GLA GS_BS_GCU ORF

<400>  116
atgcagctgc ggaacccaga gctgcacctc ggctgcgcac tggcactgcg gttcctggca       60

ctggtcagct gggacatccc cggtgcccgc gcactggaca atgggctcgc ccgcactccc      120

accatgggct ggctccactg ggagcgcttc atgtgcaatc tcgactgcca ggaggagcct      180

gacagctgca tcagcgagaa gctcttcatg gagatggcgg agctgatggt cagcgagggc      240

tggaaggacg ccgggtacga gtacctctgc atcgacgact gctggatggc gccccagcga      300

gacagcgagg gccgcctcca ggccgacccc cagcgcttcc cccacggcat ccgccagctc      360

gcgaactacg tccacagcaa ggggctcaag ctcggcatct acgcggacgt tggcaacaag      420

acgtgcgccg ggttccctgg cagcttcggc tactacgaca tcgacgcgca gacgttcgcg      480

gactggggcg tcgacctcct caagttcgac ggctgctact gcgacagcct cgagaatctc      540

gcggacggct acaagcacat gagcctcgca ctgaaccgca cgggcaggag catcgtctac      600

agctgcgagt ggcccctcta catgtggccc ttccagaagc ccaactacac agagatccgc      660

cagtactgca accactggcg caacttcgcg gacatcgacg acagctggaa gagcatcaag      720

agcatcctcg actggacgag cttcaaccag gagcgcatcg tcgacgtcgc cgggcctggt      780

ggctggaacg accctgacat gctcgtcatc ggcaacttcg gcctcagctg gaaccagcag      840

gtgactcaaa tggcactgtg ggcgatcatg gctgcccccc tcttcatgag caacgacctg      900

cggcacatca gcccccaggc caaggcactg ctccaggaca aggacgtcat cgcgatcaac      960

caggaccccc tcggcaagca gggctaccag ctgcggcagg gcgacaactt cgaagtctgg     1020

gagcgccccc tcagcggcct cgcgtgggct gtggcgatga taaaccgcca ggagatcggt     1080

ggccccagga gctacacgat cgctgtggcg agcctcggca agggggtcgc gtgcaaccct     1140

gcgtgcttca tcactcaact ccttccagtc aagcgcaagc tcggcttcta cgagtggacg     1200

agccgcctgc ggagccacat caaccccacc ggcacggtcc tcctccagct cgagaacacg     1260

atgcagatga gcctcaagga cctctag                                         1287


<210>  117
<211>  1287
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GLA GS_GCU ORF

<400>  117
atgcagctgc ggaacccaga gctgcacctg ggctgcgcac tggcactgcg gttcctggca       60

ctggtcagct gggacatccc cggtgcccgc gcactggaca atgggctggc ccgcactccc      120

accatgggct ggctgcactg ggagcgcttc atgtgcaatc tcgactgcca ggaggagcct      180

gacagctgca tcagcgagaa gctgttcatg gagatggcgg agctgatggt cagcgagggc      240

tggaaggacg ccgggtacga gtacctgtgc atcgacgact gctggatggc gccgcagcga      300

gacagcgagg gccgcctgca ggccgacccg cagcgcttcc cgcacggcat ccgccagctg      360

gcgaactacg tgcacagcaa ggggctgaag ctgggcatct acgcggacgt tggcaacaag      420

acgtgcgccg ggttcccggg cagcttcggc tactacgaca tcgacgcgca gacgttcgcg      480

gactggggcg tggacctgct gaagttcgac ggctgctact gcgacagcct ggagaatctc      540

gcggacggct acaagcacat gagcctggca ctgaaccgca cgggccgcag catcgtgtac      600

agctgcgagt ggccgctgta catgtggccg ttccagaagc cgaactacac agagatccgc      660

cagtactgca accactggcg caacttcgcg gacatcgacg acagctggaa gagcatcaag      720

agcatcctgg actggacgag cttcaaccag gagcgcatcg tggacgtggc cgggccgggt      780

ggctggaacg accctgacat gctggtgatc ggcaacttcg gcctcagctg gaaccagcag      840

gtgactcaaa tggcactgtg ggcgatcatg gctgccccgc tgttcatgag caacgacctg      900

cggcacatca gcccgcaggc caaggcactg ctgcaggaca aggacgtgat cgcgatcaac      960

caggacccgc tgggcaagca gggctaccag ctgcggcagg gcgacaactt cgaagtctgg     1020

gagcgcccgc tcagcggcct ggcgtgggct gtggcgatga taaaccgcca ggagatcggt     1080

ggcccgcgca gctacacgat cgctgtggcg agcctgggca agggggtggc gtgcaacccg     1140

gcgtgcttca tcactcaact gcttccagtg aagcgcaagc tgggcttcta cgagtggacg     1200

agccgcctgc ggagccacat caaccccacc ggcacggtgc tgctgcagct ggagaacacg     1260

atgcagatga gcctgaagga cctgtag                                         1287


<210>  118
<211>  353
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: OTC amino acid sequence

<400>  118

Met Leu Phe Asn Leu Arg Ile Leu Leu Asn Asn Ala Ala Phe Arg Asn 
1               5                   10                  15      


Gly His Asn Phe Met Val Arg Asn Phe Arg Cys Gly Gln Pro Leu Gln 
            20                  25                  30          


Asn Lys Val Gln Leu Lys Gly Arg Asp Leu Leu Thr Leu Lys Asn Phe 
        35                  40                  45              


Thr Gly Glu Glu Ile Lys Tyr Met Leu Trp Leu Ser Ala Asp Leu Lys 
    50                  55                  60                  


Phe Arg Ile Lys Gln Lys Gly Glu Tyr Leu Pro Leu Leu Gln Gly Lys 
65                  70                  75                  80  


Ser Leu Gly Met Ile Phe Glu Lys Arg Ser Thr Arg Thr Arg Leu Ser 
                85                  90                  95      


Thr Glu Thr Gly Phe Ala Leu Leu Gly Gly His Pro Cys Phe Leu Thr 
            100                 105                 110         


Thr Gln Asp Ile His Leu Gly Val Asn Glu Ser Leu Thr Asp Thr Ala 
        115                 120                 125             


Arg Val Leu Ser Ser Met Ala Asp Ala Val Leu Ala Arg Val Tyr Lys 
    130                 135                 140                 


Gln Ser Asp Leu Asp Thr Leu Ala Lys Glu Ala Ser Ile Pro Ile Ile 
145                 150                 155                 160 


Asn Gly Leu Ser Asp Leu Tyr His Pro Ile Gln Ile Leu Ala Asp Tyr 
                165                 170                 175     


Leu Thr Leu Gln Glu His Tyr Ser Ser Leu Lys Gly Leu Thr Leu Ser 
            180                 185                 190         


Trp Ile Gly Asp Gly Asn Asn Ile Leu His Ser Ile Met Met Ser Ala 
        195                 200                 205             


Ala Lys Phe Gly Met His Leu Gln Ala Ala Thr Pro Lys Gly Tyr Glu 
    210                 215                 220                 


Pro Asp Ala Ser Val Thr Lys Leu Ala Glu Gln Tyr Ala Lys Glu Asn 
225                 230                 235                 240 


Gly Thr Lys Leu Leu Leu Thr Asn Asp Pro Leu Glu Ala Ala His Gly 
                245                 250                 255     


Gly Asn Val Leu Ile Thr Asp Thr Trp Ile Ser Met Gly Gln Glu Glu 
            260                 265                 270         


Glu Lys Lys Lys Arg Leu Gln Ala Phe Gln Gly Tyr Gln Val Thr Met 
        275                 280                 285             


Lys Thr Ala Lys Val Ala Ala Ser Asp Trp Thr Phe Leu His Cys Leu 
    290                 295                 300                 


Pro Arg Lys Pro Glu Glu Val Asp Asp Glu Val Phe Tyr Ser Pro Arg 
305                 310                 315                 320 


Ser Leu Val Phe Pro Glu Ala Glu Asn Arg Lys Trp Thr Ile Met Ala 
                325                 330                 335     


Val Met Val Ser Leu Leu Thr Asp Tyr Ser Pro Gln Leu Gln Lys Pro 
            340                 345                 350         


Lys 
    


<210>  119
<211>  1062
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: OTC WT ORF

<400>  119
auguuauuua auuuacguau uuuauuaaau aaugcugcuu uucguaaugg ucauaauuuu       60

augguucgua auuuucguug uggucaaccu uuacaaaaua aaguucaauu aaaaggucgu      120

gauuuauuaa cuuuaaaaaa uuuuacuggu gaagaaauua aauauauguu augguuaucu      180

gcugauuuaa aauuucguau uaaacaaaaa ggugaauauu uaccuuuauu acaagguaaa      240

ucuuuaggua ugauuuuuga aaaacguucu acucguacuc guuuaucuac ugaaacuggu      300

uuugcuuuau uaggugguca uccuuguuuu uuaacuacuc aagauauuca uuuagguguu      360

aaugaaucuu uaacugauac ugcucguguu uuaucuucua uggcugaugc uguuuuagcu      420

cguguuuaua aacaaucuga uuuagauacu uuagcuaaag aagcuucuau uccuauuauu      480

aaugguuuau cugauuuaua ucauccuauu caaauuuuag cugauuauuu aacuuuacaa      540

gaacauuauu cuucuuuaaa agguuuaacu uuaucuugga uuggugaugg uaauaauauu      600

uuacauucua uuaugauguc ugcugcuaaa uuugguaugc auuuacaagc ugcuacuccu      660

aaagguuaug aaccugaugc uucuguuacu aaauuagcug aacaauaugc uaaagaaaau      720

gguacuaaau uauuauuaac uaaugauccu uuagaagcug cucauggugg uaauguuuua      780

auuacugaua cuuggauuuc uaugggucaa gaagaagaaa aaaaaaaacg uuuacaagcu      840

uuucaagguu aucaaguuac uaugaaaacu gcuaaaguug cugcuucuga uuggacuuuu      900

uuacauuguu uaccucguaa accugaagaa guugaugaug aaguuuuuua uucuccucgu      960

ucuuuaguuu uuccugaagc ugaaaaucgu aaauggacua uuauggcugu uaugguuucu     1020

uuauuaacug auuauucucc ucaauuacaa aaaccuaaau ag                        1062


<210>  120
<211>  1062
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: OTC BP_GCU ORF

<400>  120
atgctcttca acctccgcat cctcctcaac aacgcggcgt tccgcaacgg ccacaacttc       60

atggtccgca acttccgctg cggccagccc ctccagaaca aggtccagct caagggccgc      120

gacctcctca cgctcaagaa cttcacgggc gaggagatca agtacatgct ctggctctcg      180

gcggacctca agttccgcat caagcagaag ggcgagtacc tccccctcct ccagggcaag      240

tcgctcggca tgatcttcga gaagcgctcg acgcgcacgc gcctctcgac ggagacgggc      300

ttcgcgctcc tcggcggcca cccctgcttc ctcacgacgc aggacatcca cctcggcgtc      360

aacgagtcgc tcacggacac ggcgcgcgtc ctctcgtcga tggcggacgc ggtcctcgcg      420

cgcgtctaca agcagtcgga cctcgacacg ctcgcgaagg aggcgtcgat ccccatcatc      480

aacggcctct cggacctcta ccaccccatc cagatcctcg cggactacct cacgctccag      540

gagcactact cgtcgctcaa gggcctcacg ctctcgtgga tcggcgacgg caacaacatc      600

ctccactcga tcatgatgtc ggcggcgaag ttcggcatgc acctccaggc ggcgacgccc      660

aagggctacg agcccgacgc gtcggtcacg aagctcgcgg agcagtacgc gaaggagaac      720

ggcacgaagc tcctcctcac gaacgacccc ctcgaggcgg cgcacggcgg caacgtcctc      780

atcacggaca cgtggatctc gatgggccag gaggaggaga agaagaagcg cctccaggcg      840

ttccagggct accaggtcac gatgaagacg gcgaaggtcg cggcgtcgga ctggacgttc      900

ctccactgcc tcccccgcaa gcccgaggag gtcgacgacg aggtcttcta ctcgccccgc      960

tcgctcgtct tccccgaggc ggagaaccgc aagtggacga tcatggcggt catggtctcg     1020

ctcctcacgg actactcgcc ccagctccag aagcccaagt ag                        1062


<210>  121
<211>  1062
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: OTC GP_BP_BS_GCU ORF

<400>  121
atgctcttca atctgcggat cctcctcaac aacgctgcct tccgcaatgg gcacaacttc       60

atggtccgca acttccgctg tgggcagccc ctccagaaca aggtccagct caaggggcga      120

gacctcctca cgctcaagaa cttcacgggt gaggagatca agtacatgct ctggctcagc      180

gcggacctca agttccgcat caagcagaag ggtgagtacc ttccactcct ccagggcaag      240

tcgctcggca tgatattcga gaagcgatcg actcgcacgc gcctctcgac agagacgggc      300

ttcgcactgc tcggtggcca cccctgcttc ctgacgactc aagacatcca cctcggcgtc      360

aacgagtcgc tcacggacac ggcccgcgtc ctcagctcga tggcggacgc tgtgctcgcc      420

cgcgtctaca agcagagcga cctcgacacg ctcgcgaagg aagccagcat ccccatcatc      480

aatgggctca gcgacctcta ccaccccatc cagatcctcg cggactacct cacgctccag      540

gagcactact cgtcgctcaa ggggctcacg ctcagctgga tcggcgacgg caacaacatc      600

ctccacagca tcatgatgtc ggctgccaag ttcggcatgc acctccaggc tgccactccc      660

aaggggtacg agcctgacgc gtcggtcacg aagctcgcgg agcagtacgc gaaggagaat      720

gggacgaagc tcctcctcac gaacgacccc ctcgaagctg cccacggtgg caacgtcctc      780

atcacggaca cgtggatctc gatgggccag gaggaggaga agaagaagcg cctccaggcc      840

ttccagggct accaggtgac gatgaagacg gcgaaggtcg ctgcctcgga ctggacgttc      900

ctgcactgcc ttccacgcaa gccagaggaa gtcgacgacg aagtcttcta ctcgccccga      960

tcgctcgtct tcccagaagc cgagaaccgc aagtggacga tcatggctgt gatggtcagc     1020

ctcctcacgg actactcgcc ccagctccag aagcccaagt ag                        1062


<210>  122
<211>  1062
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: OTC GS_BS_GCU ORF

<400>  122
atgctcttca atctgcggat cctcctcaac aacgctgcct tccgcaatgg gcacaacttc       60

atggtccgca acttccgctg tgggcagccc ctccagaaca aggtccagct caaggggcga      120

gacctcctca cgctcaagaa cttcacgggt gaggagatca agtacatgct ctggctcagc      180

gcggacctca agttccgcat caagcagaag ggtgagtacc ttccactcct ccagggcaag      240

agcctcggca tgatattcga gaagaggtcg actcgcacgc gcctctcgac agagacgggc      300

ttcgcactgc tcggtggcca cccctgcttc ctgacgactc aagacatcca cctcggcgtc      360

aacgagagcc tcacggacac ggcccgcgtc ctcagcagca tggcggacgc tgtgctcgcc      420

cgcgtctaca agcagagcga cctcgacacg ctcgcgaagg aagccagcat ccccatcatc      480

aatgggctca gcgacctcta ccaccccatc cagatcctcg cggactacct cacgctccag      540

gagcactaca gcagcctcaa ggggctcacg ctcagctgga tcggcgacgg caacaacatc      600

ctccacagca tcatgatgag cgctgccaag ttcggcatgc acctccaggc tgccactccc      660

aaggggtacg agcctgacgc gagcgtcacg aagctcgcgg agcagtacgc gaaggagaat      720

gggacgaagc tcctcctcac gaacgacccc ctcgaagctg cccacggtgg caacgtcctc      780

atcacggaca cgtggatcag catgggccag gaggaggaga agaagaagcg cctccaggcc      840

ttccagggct accaggtgac gatgaagacg gcgaaggtcg ctgccagcga ctggacgttc      900

ctgcactgcc ttccacgcaa gccagaggaa gtcgacgacg aagtcttcta cagccccagg      960

agcctcgtct tcccagaagc cgagaaccgc aagtggacga tcatggctgt gatggtcagc     1020

ctcctcacgg actacagccc ccagctccag aagcccaagt ag                        1062


<210>  123
<211>  1062
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: OTC GS_GCU ORF

<400>  123
atgctgttca atctgcggat cctgctgaac aacgctgcct tccgcaatgg gcacaacttc       60

atggtgcgca acttccgctg tgggcagccc ctgcagaaca aggtgcagct gaaggggcga      120

gacctgctga cgctgaagaa cttcacgggt gaggagatca agtacatgct gtggctcagc      180

gcggacctga agttccgcat caagcagaag ggtgagtacc ttccactgct gcagggcaag      240

agcctgggca tgatattcga gaagcgctcg actcgcacgc gcctctcgac agagacgggc      300

ttcgcactgc tgggtggcca cccgtgcttc ctgacgactc aagacatcca cctgggcgtg      360

aacgagagcc tgacggacac ggcccgcgtg ctcagcagca tggcggacgc tgtgctggcc      420

cgcgtgtaca agcagagcga cctggacacg ctggcgaagg aagccagcat ccccatcatc      480

aatgggctca gcgacctgta ccacccgatc cagatcctgg cggactacct gacgctgcag      540

gagcactaca gcagcctgaa ggggctgacg ctcagctgga tcggcgacgg caacaacatc      600

ctgcacagca tcatgatgag cgctgccaag ttcggcatgc acctgcaggc tgccactccc      660

aaggggtacg agcctgacgc gagcgtgacg aagctggcgg agcagtacgc gaaggagaat      720

gggacgaagc tgctgctgac gaacgacccg ctggaagctg cccacggtgg caacgtgctg      780

atcacggaca cgtggatcag catgggccag gaggaggaga agaagaagcg cctgcaggcc      840

ttccagggct accaggtgac gatgaagacg gcgaaggtgg ctgccagcga ctggacgttc      900

ctgcactgcc ttccacgcaa gccagaggaa gtcgacgacg aagtcttcta cagcccgcgc      960

agcctggtgt tcccagaagc cgagaaccgc aagtggacga tcatggctgt gatggtcagc     1020

ctgctgacgg actacagccc gcagctgcag aagccgaagt ag                        1062


<210>  124
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: PAH amino acid sequence

<400>  124

Met Ser Thr Ala Val Leu Glu Asn Pro Gly Leu Gly Arg Lys Leu Ser 
1               5                   10                  15      


Asp Phe Gly Gln Glu Thr Ser Tyr Ile Glu Asp Asn Cys Asn Gln Asn 
            20                  25                  30          


Gly Ala Ile Ser Leu Ile Phe Ser Leu Lys Glu Glu Val Gly Ala Leu 
        35                  40                  45              


Ala Lys Val Leu Arg Leu Phe Glu Glu Asn Asp Val Asn Leu Thr His 
    50                  55                  60                  


Ile Glu Ser Arg Pro Ser Arg Leu Lys Lys Asp Glu Tyr Glu Phe Phe 
65                  70                  75                  80  


Thr His Leu Asp Lys Arg Ser Leu Pro Ala Leu Thr Asn Ile Ile Lys 
                85                  90                  95      


Ile Leu Arg His Asp Ile Gly Ala Thr Val His Glu Leu Ser Arg Asp 
            100                 105                 110         


Lys Lys Lys Asp Thr Val Pro Trp Phe Pro Arg Thr Ile Gln Glu Leu 
        115                 120                 125             


Asp Arg Phe Ala Asn Gln Ile Leu Ser Tyr Gly Ala Glu Leu Asp Ala 
    130                 135                 140                 


Asp His Pro Gly Phe Lys Asp Pro Val Tyr Arg Ala Arg Arg Lys Gln 
145                 150                 155                 160 


Phe Ala Asp Ile Ala Tyr Asn Tyr Arg His Gly Gln Pro Ile Pro Arg 
                165                 170                 175     


Val Glu Tyr Met Glu Glu Glu Lys Lys Thr Trp Gly Thr Val Phe Lys 
            180                 185                 190         


Thr Leu Lys Ser Leu Tyr Lys Thr His Ala Cys Tyr Glu Tyr Asn His 
        195                 200                 205             


Ile Phe Pro Leu Leu Glu Lys Tyr Cys Gly Phe His Glu Asp Asn Ile 
    210                 215                 220                 


Pro Gln Leu Glu Asp Val Ser Gln Phe Leu Gln Thr Cys Thr Gly Phe 
225                 230                 235                 240 


Arg Leu Arg Pro Val Ala Gly Leu Leu Ser Ser Arg Asp Phe Leu Gly 
                245                 250                 255     


Gly Leu Ala Phe Arg Val Phe His Cys Thr Gln Tyr Ile Arg His Gly 
            260                 265                 270         


Ser Lys Pro Met Tyr Thr Pro Glu Pro Asp Ile Cys His Glu Leu Leu 
        275                 280                 285             


Gly His Val Pro Leu Phe Ser Asp Arg Ser Phe Ala Gln Phe Ser Gln 
    290                 295                 300                 


Glu Ile Gly Leu Ala Ser Leu Gly Ala Pro Asp Glu Tyr Ile Glu Lys 
305                 310                 315                 320 


Leu Ala Thr Ile Tyr Trp Phe Thr Val Glu Phe Gly Leu Cys Lys Gln 
                325                 330                 335     


Gly Asp Ser Ile Lys Ala Tyr Gly Ala Gly Leu Leu Ser Ser Phe Gly 
            340                 345                 350         


Glu Leu Gln Tyr Cys Leu Ser Glu Lys Pro Lys Leu Leu Pro Leu Glu 
        355                 360                 365             


Leu Glu Lys Thr Ala Ile Gln Asn Tyr Thr Val Thr Glu Phe Gln Pro 
    370                 375                 380                 


Leu Tyr Tyr Val Ala Glu Ser Phe Asn Asp Ala Lys Glu Lys Val Arg 
385                 390                 395                 400 


Asn Phe Ala Ala Thr Ile Pro Arg Pro Phe Ser Val Arg Tyr Asp Pro 
                405                 410                 415     


Tyr Thr Gln Arg Ile Glu Val Leu Asp Asn Thr Gln Gln Leu Lys Ile 
            420                 425                 430         


Leu Ala Asp Ser Ile Asn Ser Glu Ile Gly Ile Leu Cys Ser Ala Leu 
        435                 440                 445             


Gln Lys Ile 
    450     


<210>  125
<211>  1356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PAH WT ORF

<400>  125
augucuacug cuguuuuaga aaauccuggu uuaggucgua aauuaucuga uuuuggucaa       60

gaaacuucuu auauugaaga uaauuguaau caaaauggug cuauuucuuu aauuuuuucu      120

uuaaaagaag aaguuggugc uuuagcuaaa guuuuacguu uauuugaaga aaaugauguu      180

aauuuaacuc auauugaauc ucguccuucu cguuuaaaaa aagaugaaua ugaauuuuuu      240

acucauuuag auaaacguuc uuuaccugcu uuaacuaaua uuauuaaaau uuuacgucau      300

gauauuggug cuacuguuca ugaauuaucu cgugauaaaa aaaaagauac uguuccuugg      360

uuuccucgua cuauucaaga auuagaucgu uuugcuaauc aaauuuuauc uuauggugcu      420

gaauuagaug cugaucaucc ugguuuuaaa gauccuguuu aucgugcucg ucguaaacaa      480

uuugcugaua uugcuuauaa uuaucgucau ggucaaccua uuccucgugu ugaauauaug      540

gaagaagaaa aaaaaacuug ggguacuguu uuuaaaacuu uaaaaucuuu auauaaaacu      600

caugcuuguu augaauauaa ucauauuuuu ccuuuauuag aaaaauauug ugguuuucau      660

gaagauaaua uuccucaauu agaagauguu ucucaauuuu uacaaacuug uacugguuuu      720

cguuuacguc cuguugcugg uuuauuaucu ucucgugauu uuuuaggugg uuuagcuuuu      780

cguguuuuuc auuguacuca auauauucgu caugguucua aaccuaugua uacuccugaa      840

ccugauauuu gucaugaauu auuaggucau guuccuuuau uuucugaucg uucuuuugcu      900

caauuuucuc aagaaauugg uuuagcuucu uuaggugcuc cugaugaaua uauugaaaaa      960

uuagcuacua uuuauugguu uacuguugaa uuugguuuau guaaacaagg ugauucuauu     1020

aaagcuuaug gugcugguuu auuaucuucu uuuggugaau uacaauauug uuuaucugaa     1080

aaaccuaaau uauuaccuuu agaauuagaa aaaacugcua uucaaaauua uacuguuacu     1140

gaauuucaac cuuuauauua uguugcugaa ucuuuuaaug augcuaaaga aaaaguucgu     1200

aauuuugcug cuacuauucc ucguccuuuu ucuguucguu augauccuua uacucaacgu     1260

auugaaguuu uagauaauac ucaacaauua aaaauuuuag cugauucuau uaauucugaa     1320

auugguauuu uauguucugc uuuacaaaaa auuuag                               1356


<210>  126
<211>  1356
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PAH BP_GCU ORF

<400>  126
atgtcgacgg cggtcctcga gaaccccggc ctcggccgca agctctcgga cttcggccag       60

gagacgtcgt acatcgagga caactgcaac cagaacggcg cgatctcgct catcttctcg      120

ctcaaggagg aggtcggcgc gctcgcgaag gtcctccgcc tcttcgagga gaacgacgtc      180

aacctcacgc acatcgagtc gcgcccctcg cgcctcaaga aggacgagta cgagttcttc      240

acgcacctcg acaagcgctc gctccccgcg ctcacgaaca tcatcaagat cctccgccac      300

gacatcggcg cgacggtcca cgagctctcg cgcgacaaga agaaggacac ggtcccctgg      360

ttcccccgca cgatccagga gctcgaccgc ttcgcgaacc agatcctctc gtacggcgcg      420

gagctcgacg cggaccaccc cggcttcaag gaccccgtct accgcgcgcg ccgcaagcag      480

ttcgcggaca tcgcgtacaa ctaccgccac ggccagccca tcccccgcgt cgagtacatg      540

gaggaggaga agaagacgtg gggcacggtc ttcaagacgc tcaagtcgct ctacaagacg      600

cacgcgtgct acgagtacaa ccacatcttc cccctcctcg agaagtactg cggcttccac      660

gaggacaaca tcccccagct cgaggacgtc tcgcagttcc tccagacgtg cacgggcttc      720

cgcctccgcc ccgtcgcggg cctcctctcg tcgcgcgact tcctcggcgg cctcgcgttc      780

cgcgtcttcc actgcacgca gtacatccgc cacggctcga agcccatgta cacgcccgag      840

cccgacatct gccacgagct cctcggccac gtccccctct tctcggaccg ctcgttcgcg      900

cagttctcgc aggagatcgg cctcgcgtcg ctcggcgcgc ccgacgagta catcgagaag      960

ctcgcgacga tctactggtt cacggtcgag ttcggcctct gcaagcaggg cgactcgatc     1020

aaggcgtacg gcgcgggcct cctctcgtcg ttcggcgagc tccagtactg cctctcggag     1080

aagcccaagc tcctccccct cgagctcgag aagacggcga tccagaacta cacggtcacg     1140

gagttccagc ccctctacta cgtcgcggag tcgttcaacg acgcgaagga gaaggtccgc     1200

aacttcgcgg cgacgatccc ccgccccttc tcggtccgct acgaccccta cacgcagcgc     1260

atcgaggtcc tcgacaacac gcagcagctc aagatcctcg cggactcgat caactcggag     1320

atcggcatcc tctgctcggc gctccagaag atctag                               1356


<210>  127
<211>  1356
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PAH GP_BP_BS_GCU ORF

<400>  127
atgtcgactg ctgtgctcga gaaccctggc ctcggccgca agctcagcga cttcggccag       60

gagacgtcgt acatcgagga caactgcaac cagaatggtg ccatctcgct catcttctcg      120

ctcaaggagg aagttggtgc actggcgaag gtcctgcggc tcttcgagga gaacgacgtc      180

aatctcacgc acatcgagtc gcgcccctca cgcctcaaga aggacgagta cgagttcttc      240

acgcacctcg acaagcgatc gcttccagca ctgacgaaca tcatcaagat cctgcggcac      300

gacatcggtg ccacggtcca cgagctcagc cgagacaaga agaaggacac ggttccctgg      360

ttcccccgca cgatccagga gctggaccgc ttcgcgaacc agatcctcag ctacggtgcc      420

gagctggacg cggaccaccc tggcttcaag gaccccgtct accgcgcccg ccgcaagcag      480

ttcgcggaca tcgcgtacaa ctaccgccac ggccagccca tcccccgcgt cgagtacatg      540

gaggaggaga agaagacgtg gggcacggtc ttcaagacgc tcaagtcgct ctacaagacg      600

cacgcgtgct acgagtacaa ccacatcttc cccctcctcg agaagtactg tgggttccac      660

gaggacaaca tcccccagct cgaggacgtc agccagttcc tgcagacgtg cacgggcttc      720

cgcctgcggc ccgtcgccgg gctcctcagc tcgcgagact tcctgggtgg cctcgcgttc      780

cgcgtcttcc actgcactca atacatccgc cacggctcga agcccatgta cactccagag      840

cctgacatct gccacgagct gctcggccac gttcccctct tctcggaccg atcgttcgcg      900

cagttctcgc aggagatcgg cctcgcgtcg ctcggtgccc ctgacgagta catcgagaag      960

ctcgcgacga tctactggtt cacggtcgag ttcggcctct gcaagcaggg cgacagcatc     1020

aaggcgtacg gtgccgggct cctcagctcg ttcggtgagc tgcagtactg cctcagcgag     1080

aagcccaagc tccttccact cgagctggag aagacggcga tccagaacta cacggtcaca     1140

gagttccagc ccctctacta cgtcgcggag tcgttcaacg acgcgaagga gaaggtccgc     1200

aacttcgctg ccacgatccc ccgccccttc tcggtccgct acgaccccta cactcaacgc     1260

atcgaagtcc tcgacaacac tcaacagctc aagatcctcg cggacagcat caactcggag     1320

atcggcatcc tctgctcggc actgcagaag atctag                               1356


<210>  128
<211>  1356
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PAH GS_BS_GCU ORF

<400>  128
atgtcgactg ctgtgctcga gaaccctggc ctcggccgca agctcagcga cttcggccag       60

gagacgagct acatcgagga caactgcaac cagaatggtg ccatcagcct catcttcagc      120

ctcaaggagg aagttggtgc actggcgaag gtcctgcggc tcttcgagga gaacgacgtc      180

aatctcacgc acatcgagag ccgcccctca cgcctcaaga aggacgagta cgagttcttc      240

acgcacctcg acaagaggag ccttccagca ctgacgaaca tcatcaagat cctgcggcac      300

gacatcggtg ccacggtcca cgagctcagc cgagacaaga agaaggacac ggttccctgg      360

ttcccccgca cgatccagga gctggaccgc ttcgcgaacc agatcctcag ctacggtgcc      420

gagctggacg cggaccaccc tggcttcaag gaccccgtct accgcgcccg ccgcaagcag      480

ttcgcggaca tcgcgtacaa ctaccgccac ggccagccca tcccccgcgt cgagtacatg      540

gaggaggaga agaagacgtg gggcacggtc ttcaagacgc tcaagagcct ctacaagacg      600

cacgcgtgct acgagtacaa ccacatcttc cccctcctcg agaagtactg tgggttccac      660

gaggacaaca tcccccagct cgaggacgtc agccagttcc tgcagacgtg cacgggcttc      720

cgcctgcggc ccgtcgccgg gctcctcagc agccgagact tcctgggtgg cctcgcgttc      780

cgcgtcttcc actgcactca atacatccgc cacggcagca agcccatgta cactccagag      840

cctgacatct gccacgagct gctcggccac gttcccctct tcagcgacag gagcttcgcg      900

cagttcagcc aggagatcgg cctcgcgagc ctcggtgccc ctgacgagta catcgagaag      960

ctcgcgacga tctactggtt cacggtcgag ttcggcctct gcaagcaggg cgacagcatc     1020

aaggcgtacg gtgccgggct cctcagcagc ttcggtgagc tgcagtactg cctcagcgag     1080

aagcccaagc tccttccact cgagctggag aagacggcga tccagaacta cacggtcaca     1140

gagttccagc ccctctacta cgtcgcggag agcttcaacg acgcgaagga gaaggtccgc     1200

aacttcgctg ccacgatccc ccgccccttc agcgtccgct acgaccccta cactcaacgc     1260

atcgaagtcc tcgacaacac tcaacagctc aagatcctcg cggacagcat caacagcgag     1320

atcggcatcc tctgcagcgc actgcagaag atctag                               1356


<210>  129
<211>  1356
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PAH GS_GCU ORF

<400>  129
atgtcgactg ctgtgctgga gaacccgggc ctgggccgca agctcagcga cttcggccag       60

gagacgagct acatcgagga caactgcaac cagaatggtg ccatcagcct gatcttcagc      120

ctgaaggagg aagttggtgc actggcgaag gtgctgcggc tgttcgagga gaacgacgtg      180

aatctcacgc acatcgagag ccgcccctca cgcctgaaga aggacgagta cgagttcttc      240

acgcacctgg acaagcgcag ccttccagca ctgacgaaca tcatcaagat cctgcggcac      300

gacatcggtg ccacggtgca cgagctcagc cgagacaaga agaaggacac ggttccctgg      360

ttcccgcgca cgatccagga gctggaccgc ttcgcgaacc agatcctcag ctacggtgcc      420

gagctggacg cggaccaccc gggcttcaag gacccggtgt accgcgcccg ccgcaagcag      480

ttcgcggaca tcgcgtacaa ctaccgccac ggccagccca tcccccgcgt ggagtacatg      540

gaggaggaga agaagacgtg gggcacggtg ttcaagacgc tgaagagcct gtacaagacg      600

cacgcgtgct acgagtacaa ccacatcttc ccgctgctgg agaagtactg tgggttccac      660

gaggacaaca tcccccagct ggaggacgtc agccagttcc tgcagacgtg cacgggcttc      720

cgcctgcggc cggtggccgg gctgctcagc agccgagact tcctgggtgg cctggcgttc      780

cgcgtgttcc actgcactca atacatccgc cacggcagca agccgatgta cactccagag      840

cctgacatct gccacgagct gctgggccac gttcccctgt tcagcgaccg cagcttcgcg      900

cagttcagcc aggagatcgg cctggcgagc ctgggtgccc ctgacgagta catcgagaag      960

ctggcgacga tctactggtt cacggtggag ttcggcctgt gcaagcaggg cgacagcatc     1020

aaggcgtacg gtgccgggct gctcagcagc ttcggtgagc tgcagtactg cctcagcgag     1080

aagccgaagc tgcttccact ggagctggag aagacggcga tccagaacta cacggtgaca     1140

gagttccagc ccctgtacta cgtggcggag agcttcaacg acgcgaagga gaaggtgcgc     1200

aacttcgctg ccacgatccc ccgcccgttc agcgtgcgct acgacccgta cactcaacgc     1260

atcgaagtcc tggacaacac tcaacagctg aagatcctgg cggacagcat caacagcgag     1320

atcggcatcc tgtgcagcgc actgcagaag atctag                               1356


<210>  130
<211>  146
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: TTR amino acid sequence

<400>  130

Met Ala Ser His Arg Leu Leu Leu Leu Cys Leu Ala Gly Leu Val Phe 
1               5                   10                  15      


Val Ser Glu Ala Gly Pro Thr Gly Thr Gly Glu Ser Lys Cys Pro Leu 
            20                  25                  30          


Met Val Lys Val Leu Asp Ala Val Arg Gly Ser Pro Ala Ile Asn Val 
        35                  40                  45              


Ala Val His Val Phe Arg Lys Ala Ala Asp Asp Thr Trp Glu Pro Phe 
    50                  55                  60                  


Ala Ser Gly Lys Thr Ser Glu Ser Gly Glu Leu His Gly Leu Thr Thr 
65                  70                  75                  80  


Glu Glu Glu Phe Val Glu Gly Ile Tyr Lys Val Glu Ile Asp Thr Lys 
                85                  90                  95      


Ser Tyr Trp Lys Ala Leu Gly Ile Ser Pro Phe His Glu His Ala Glu 
            100                 105                 110         


Val Val Phe Thr Ala Asn Asp Ser Gly Pro Arg Arg Tyr Thr Ile Ala 
        115                 120                 125             


Ala Leu Leu Ser Pro Tyr Ser Tyr Ser Thr Thr Ala Val Val Thr Asn 
    130                 135                 140                 


Pro Lys 
145     


<210>  131
<211>  441
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: TTR WT ORF

<400>  131
auggcuucuc aucguuuauu auuauuaugu uuagcugguu uaguuuuugu uucugaagcu       60

gguccuacug guacugguga aucuaaaugu ccuuuaaugg uuaaaguuuu agaugcuguu      120

cgugguucuc cugcuauuaa uguugcuguu cauguuuuuc guaaagcugc ugaugauacu      180

ugggaaccuu uugcuucugg uaaaacuucu gaaucuggug aauuacaugg uuuaacuacu      240

gaagaagaau uuguugaagg uauuuauaaa guugaaauug auacuaaauc uuauuggaaa      300

gcuuuaggua uuucuccuuu ucaugaacau gcugaaguug uuuuuacugc uaaugauucu      360

gguccucguc guuauacuau ugcugcuuua uuaucuccuu auucuuauuc uacuacugcu      420

guuguuacua auccuaaaua g                                                441


<210>  132
<211>  441
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: TTR BP_GCU ORF

<400>  132
atggcgtcgc accgcctcct cctcctctgc ctcgcgggcc tcgtcttcgt ctcggaggcg       60

ggccccacgg gcacgggcga gtcgaagtgc cccctcatgg tcaaggtcct cgacgcggtc      120

cgcggctcgc ccgcgatcaa cgtcgcggtc cacgtcttcc gcaaggcggc ggacgacacg      180

tgggagccct tcgcgtcggg caagacgtcg gagtcgggcg agctccacgg cctcacgacg      240

gaggaggagt tcgtcgaggg catctacaag gtcgagatcg acacgaagtc gtactggaag      300

gcgctcggca tctcgccctt ccacgagcac gcggaggtcg tcttcacggc gaacgactcg      360

ggcccccgcc gctacacgat cgcggcgctc ctctcgccct actcgtactc gacgacggcg      420

gtcgtcacga accccaagta g                                                441


<210>  133
<211>  441
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: TTR GP_BP_BS_GCU ORF

<400>  133
atggcgtcgc accgcctcct cctcctctgc ctcgccgggc tcgtcttcgt cagcgaagcc       60

gggcccaccg gcacgggtga gtcgaagtgc cccctcatgg tcaaggtcct cgacgctgtg      120

cgcggctcgc ctgcgatcaa cgtcgctgtg cacgtcttcc gcaaggctgc cgacgacacg      180

tgggagccct tcgcgtcggg caagacgtcg gagtcgggtg agctgcacgg cctcacgaca      240

gaggaggagt tcgtcgaggg catctacaag gtcgagatcg acacgaagtc gtactggaag      300

gcactgggca tctcgccctt ccacgagcac gcggaagtcg tcttcacggc gaacgactcg      360

ggcccccgcc gctacacgat cgctgcactg ctcagcccct actcgtactc gactacggct      420

gtggtcacga accccaagta g                                                441


<210>  134
<211>  441
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: TTR GS_BS_GCU ORF

<400>  134
atggcgagcc accgcctcct cctcctctgc ctcgccgggc tcgtcttcgt cagcgaagcc       60

gggcccaccg gcacgggtga gagcaagtgc cccctcatgg tcaaggtcct cgacgctgtg      120

cgcggcagcc ctgcgatcaa cgtcgctgtg cacgtcttcc gcaaggctgc cgacgacacg      180

tgggagccct tcgcgagcgg caagacgagc gagagcggtg agctgcacgg cctcacgaca      240

gaggaggagt tcgtcgaggg catctacaag gtcgagatcg acacgaagag ctactggaag      300

gcactgggca tcagcccctt ccacgagcac gcggaagtcg tcttcacggc gaacgacagc      360

ggcccccgcc gctacacgat cgctgcactg ctcagcccct acagctactc gactacggct      420

gtggtcacga accccaagta g                                                441


<210>  135
<211>  441
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: TTR GS_GCU ORF

<400>  135
atggcgagcc accgcctgct gctgctgtgc ctggccgggc tggtgttcgt cagcgaagcc       60

gggcccaccg gcacgggtga gagcaagtgc ccgctgatgg tgaaggtgct ggacgctgtg      120

cgcggcagcc cggcgatcaa cgtggctgtg cacgtgttcc gcaaggctgc cgacgacacg      180

tgggagccgt tcgcgagcgg caagacgagc gagagcggtg agctgcacgg cctgacgaca      240

gaggaggagt tcgtggaggg catctacaag gtggagatcg acacgaagag ctactggaag      300

gcactgggca tcagcccgtt ccacgagcac gcggaagtcg tgttcacggc gaacgacagc      360

ggcccgcgcc gctacacgat cgctgcactg ctcagcccgt acagctactc gactacggct      420

gtggtgacga acccgaagta g                                                441


<210>  136
<211>  1257
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: FAH BS_GCU

<400>  136
augagcuuca uccccgucgc ggaggacagc gacuucccca uccacaaccu ccccuacggc       60

gucuucagca cgcgcggcga cccccgcccc cgcaucggcg ucgcgaucgg cgaccagauc      120

cucgaccuca gcaucaucaa gcaccucuuc acgggccccg uccucagcaa gcaccaggac      180

gucuucaacc agcccacgcu caacagcuuc augggccucg gccaggcggc guggaaggag      240

gcgcgcgucu uccuccagaa ccuccucagc gucagccagg cgcgccuccg cgacgacacg      300

gagcuccgca agugcgcguu caucagccag gcgagcgcga cgaugcaccu ccccgcgacg      360

aucggcgacu acacggacuu cuacagcagc cgccagcacg cgacgaacgu cggcaucaug      420

uuccgcgaca aggagaacgc gcucaugccc aacuggcucc accuccccgu cggcuaccac      480

ggccgcgcga gcagcgucgu cgucagcggc acgcccaucc gccgccccau gggccagaug      540

aagcccgacg acagcaagcc ccccgucuac ggcgcgugca agcuccucga cauggagcuc      600

gagauggcgu ucuucgucgg ccccggcaac cgccucggcg agcccauccc caucagcaag      660

gcgcacgagc acaucuucgg caugguccuc augaacgacu ggagcgcgcg cgacauccag      720

aagugggagu acgucccccu cggccccuuc cucggcaaga gcuucggcac gacggucagc      780

cccugggucg uccccaugga cgcgcucaug cccuucgcgg uccccaaccc caagcaggac      840

ccccgccccc uccccuaccu cugccacgac gagcccuaca cguucgacau caaccucagc      900

gucaaccuca agggcgaggg caugagccag gcggcgacga ucugcaagag caacuucaag      960

uacauguacu ggacgaugcu ccagcagcuc acgcaccaca gcgucaacgg cugcaaccuc     1020

cgccccggcg accuccucgc gagcggcacg aucagcggcc ccgagcccga gaacuucggc     1080

agcaugcucg agcucagcug gaagggcacg aagcccaucg accucggcaa cggccagacg     1140

cgcaaguucc uccucgacgg cgacgagguc aucaucacgg gcuacugcca gggcgacggc     1200

uaccgcaucg gcuucggcca gugcgcgggc aagguccucc ccgcgcuccu ccccuag        1257


<210>  137
<211>  1395
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GABRD BS_GCU

<400>  137
augagcgagg cgacgccccu cgaccgcaac gacagcgaga acacgggcgg ccucaucagc       60

cgcccccacc ccugggacca gagccccagc ugcguccagg aggaccgcgc gaugaacgac      120

aucggcgacu acgucggcag caaccucgag aucagcuggc uccccaaccu cgacggccuc      180

aucgcgggcu acgcgcgcaa cuuccgcccc ggcaucggcg gcccccccgu caacgucgcg      240

cucgcgcucg aggucgcgag caucgaccac aucagcgagg cgaacaugga guacacgaug      300

acggucuucc uccaccagag cuggcgcgac agccgccuca gcuacaacca cacgaacgag      360

acgcucggcc ucgacagccg cuucgucgac aagcucuggc uccccgacac guucaucguc      420

aacgcgaaga gcgcgugguu ccacgacguc acggucgaga acaagcucau ccgccuccag      480

cccgacggcg ucauccucua cagcauccgc aucacgagca cggucgcgug cgacauggac      540

cucgcgaagu accccaugga cgagcaggag ugcaugcucg accucgagag cuacggcuac      600

agcagcgagg acaucgucua cuacuggagc gagagccagg agcacaucca cggccucgac      660

aagcuccagc ucgcgcaguu cacgaucacg agcuaccgcu ucacgacgga gcucaugaac      720

uucaagagcg cgggccaguu cccccgccuc agccuccacu uccaccuccg ccgcaaccgc      780

ggcgucuaca ucauccagag cuacaugccc agcguccucc ucgucgcgau gagcuggguc      840

agcuucugga ucagccaggc ggcggucccc gcgcgcguca gccucggcau cacgacgguc      900

cucacgauga cgacgcucau ggucagcgcg cgcagcagcc ucccccgcgc gagcgcgauc      960

aaggcgcucg acgucuacuu cuggaucugc uacgucuucg ucuucgcggc gcucgucgag     1020

uacgcguucg cgcacuucaa cgcggacuac cgcaagaagc agaaggcgaa ggucaagguc     1080

agccgccccc gcgcggagau ggacguccgc aacgcgaucg uccucuucag ccucagcgcg     1140

gcgggcguca cgcaggagcu cgcgaucagc cgccgccagc gccgcguccc cggcaaccuc     1200

augggcagcu accgcagcgu cggcgucgag acgggcgaga cgaagaagga gggcgcggcg     1260

cgcagcggcg gccagggcgg cauccgcgcg cgccuccgcc ccaucgacgc ggacacgauc     1320

gacaucuacg cgcgcgcggu cuuccccgcg gcguucgcgg cggucaacgu caucuacugg     1380

gcggcguacg cguag                                                      1395


<210>  138
<211>  951
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GAPDH BS_GCU

<400>  138
augggcaagg ucaaggucgg cgucaacggc uucggccgca ucggccgccu cgucacgcgc       60

gcggcguuca acagcggcaa ggucgacauc gucgcgauca acgaccccuu caucgaccuc      120

aacuacaugg cggagaacgg caagcucguc aucaacggca accccaucac gaucuuccag      180

gagcgcgacc ccagcaagau caaguggggc gacgcgggcg cggaguacgu cgucgagagc      240

acgggcgucu ucacgacgau ggagaaggcg ggcgcgcacc uccagggcgg cgcgaagcgc      300

gucaucauca gcgcgcccag cgcggacgcg cccauguucg ucaugggcgu caaccacgag      360

aaguacgaca acagccucaa gaucaucagc aacgcgagcu gcacgacgaa cugccucgcg      420

ccccucgcga aggucaucca cgacaacuuc ggcaucgucg agggccucau gacgacgguc      480

cacgcgauca cggcgacgca gaagacgguc gacggcccca gcggcaagcu cuggcgcgac      540

ggccgcggcg cgcuccagaa caucaucccc gcgagcacgg gcgcggcgaa ggcggucggc      600

aaggucaucc ccgagcucaa cggcaagcuc acgggcaugg cguuccgcgu ccccacggcg      660

aacgucagcg ucgucgaccu cacgugccgc cucgagaagc ccgcgaagua cgacgacauc      720

aagaaggucg ucaagcaggc gagcgagggc ccccucaagg gcauccucgg cuacacggag      780

caccaggucg ucagcagcga cuucaacagc gacacgcaca gcagcacguu cgacgcgggc      840

gcgggcaucg cgcucaacga ccacuucguc aagcucauca gcugguacga caacgaguuc      900

ggcuacagca accgcgucgu cgaccucaug gcgcacaugg cgagcaagua g               951


<210>  139
<211>  1608
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GBA1 BS_GCU

<400>  139
auggaguuca gcagccccag ccgcgaggag ugccccaagc cccucagccg cgucagcauc       60

auggcgggca gccucacggg ccuccuccuc cuccaggcgg ucagcugggc gagcggcgcg      120

cgccccugca uccccaagag cuucggcuac agcagcgucg ucugcgucug caacgcgacg      180

uacugcgaca gcuucgaccc ccccacguuc cccgcgcucg gcacguucag ccgcuacgag      240

agcacgcgca gcggccgccg cauggagcuc agcaugggcc ccauccaggc gaaccacacg      300

ggcacgggcc uccuccucac gcuccagccc gagcagaagu uccagaaggu caagggcuuc      360

ggcggcgcga ugacggacgc ggcggcgcuc aacauccucg cgcucagccc ccccgcgcag      420

aaccuccucc ucaagagcua cuucagcgag gagggcaucg gcuacaacau cauccgcguc      480

cccauggcga gcugcgacuu cagcauccgc acguacacgu acgcggacac gcccgacgac      540

uuccagcucc acaacuucag ccuccccgag gaggacacga agcucaagau cccccucauc      600

caccgcgcgc uccagcucgc gcagcgcccc gucagccucc ucgcgagccc cuggacgagc      660

cccacguggc ucaagacgaa cggcgcgguc aacggcaagg gcagccucaa gggccagccc      720

ggcgacaucu accaccagac gugggcgcgc uacuucguca aguuccucga cgcguacgcg      780

gagcacaagc uccaguucug ggcggucacg gcggagaacg agcccagcgc gggccuccuc      840

agcggcuacc ccuuccagug ccucggcuuc acgcccgagc accagcgcga cuucaucgcg      900

cgcgaccucg gccccacgcu cgcgaacagc acgcaccaca acguccgccu ccucaugcuc      960

gacgaccagc gccuccuccu cccccacugg gcgaaggucg uccucacgga ccccgaggcg     1020

gcgaaguacg uccacggcau cgcgguccac ugguaccucg acuuccucgc gcccgcgaag     1080

gcgacgcucg gcgagacgca ccgccucuuc cccaacacga ugcucuucgc gagcgaggcg     1140

ugcgucggca gcaaguucug ggagcagagc guccgccucg gcagcuggga ccgcggcaug     1200

caguacagcc acagcaucau cacgaaccuc cucuaccacg ucgucggcug gacggacugg     1260

aaccucgcgc ucaaccccga gggcggcccc aacugggucc gcaacuucgu cgacagcccc     1320

aucaucgucg acaucacgaa ggacacguuc uacaagcagc ccauguucua ccaccucggc     1380

cacuucagca aguucauccc cgagggcagc cagcgcgucg gccucgucgc gagccagaag     1440

aacgaccucg acgcggucgc gcucaugcac cccgacggca gcgcggucgu cgucguccuc     1500

aaccgcagca gcaaggacgu cccccucacg aucaaggacc ccgcggucgg cuuccucgag     1560

acgaucagcc ccggcuacag cauccacacg uaccucuggc gccgcuag                  1608


<210>  140
<211>  1287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GLA BS_GCU

<400>  140
augcagcucc gcaaccccga gcuccaccuc ggcugcgcgc ucgcgcuccg cuuccucgcg       60

cucgucagcu gggacauccc cggcgcgcgc gcgcucgaca acggccucgc gcgcacgccc      120

acgaugggcu ggcuccacug ggagcgcuuc augugcaacc ucgacugcca ggaggagccc      180

gacagcugca ucagcgagaa gcucuucaug gagauggcgg agcucauggu cagcgagggc      240

uggaaggacg cgggcuacga guaccucugc aucgacgacu gcuggauggc gccccagcgc      300

gacagcgagg gccgccucca ggcggacccc cagcgcuucc cccacggcau ccgccagcuc      360

gcgaacuacg uccacagcaa gggccucaag cucggcaucu acgcggacgu cggcaacaag      420

acgugcgcgg gcuuccccgg cagcuucggc uacuacgaca ucgacgcgca gacguucgcg      480

gacuggggcg ucgaccuccu caaguucgac ggcugcuacu gcgacagccu cgagaaccuc      540

gcggacggcu acaagcacau gagccucgcg cucaaccgca cgggccgcag caucgucuac      600

agcugcgagu ggccccucua cauguggccc uuccagaagc ccaacuacac ggagauccgc      660

caguacugca accacuggcg caacuucgcg gacaucgacg acagcuggaa gagcaucaag      720

agcauccucg acuggacgag cuucaaccag gagcgcaucg ucgacgucgc gggccccggc      780

ggcuggaacg accccgacau gcucgucauc ggcaacuucg gccucagcug gaaccagcag      840

gucacgcaga uggcgcucug ggcgaucaug gcggcgcccc ucuucaugag caacgaccuc      900

cgccacauca gcccccaggc gaaggcgcuc cuccaggaca aggacgucau cgcgaucaac      960

caggaccccc ucggcaagca gggcuaccag cuccgccagg gcgacaacuu cgaggucugg     1020

gagcgccccc ucagcggccu cgcgugggcg gucgcgauga ucaaccgcca ggagaucggc     1080

ggcccccgca gcuacacgau cgcggucgcg agccucggca agggcgucgc gugcaacccc     1140

gcgugcuuca ucacgcagcu ccuccccguc aagcgcaagc ucggcuucua cgaguggacg     1200

agccgccucc gcagccacau caaccccacg ggcacggucc uccuccagcu cgagaacacg     1260

augcagauga gccucaagga ccucuag                                         1287


<210>  141
<211>  1062
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: OTC BS_GCU

<400>  141
augcucuuca accuccgcau ccuccucaac aacgcggcgu uccgcaacgg ccacaacuuc       60

augguccgca acuuccgcug cggccagccc cuccagaaca agguccagcu caagggccgc      120

gaccuccuca cgcucaagaa cuucacgggc gaggagauca aguacaugcu cuggcucagc      180

gcggaccuca aguuccgcau caagcagaag ggcgaguacc ucccccuccu ccagggcaag      240

agccucggca ugaucuucga gaagcgcagc acgcgcacgc gccucagcac ggagacgggc      300

uucgcgcucc ucggcggcca ccccugcuuc cucacgacgc aggacaucca ccucggcguc      360

aacgagagcc ucacggacac ggcgcgcguc cucagcagca uggcggacgc gguccucgcg      420

cgcgucuaca agcagagcga ccucgacacg cucgcgaagg aggcgagcau ccccaucauc      480

aacggccuca gcgaccucua ccaccccauc cagauccucg cggacuaccu cacgcuccag      540

gagcacuaca gcagccucaa gggccucacg cucagcugga ucggcgacgg caacaacauc      600

cuccacagca ucaugaugag cgcggcgaag uucggcaugc accuccaggc ggcgacgccc      660

aagggcuacg agcccgacgc gagcgucacg aagcucgcgg agcaguacgc gaaggagaac      720

ggcacgaagc uccuccucac gaacgacccc cucgaggcgg cgcacggcgg caacguccuc      780

aucacggaca cguggaucag caugggccag gaggaggaga agaagaagcg ccuccaggcg      840

uuccagggcu accaggucac gaugaagacg gcgaaggucg cggcgagcga cuggacguuc      900

cuccacugcc ucccccgcaa gcccgaggag gucgacgacg aggucuucua cagcccccgc      960

agccucgucu uccccgaggc ggagaaccgc aaguggacga ucauggcggu cauggucagc     1020

cuccucacgg acuacagccc ccagcuccag aagcccaagu ag                        1062


<210>  142
<211>  1356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PAH BS_GCU

<400>  142
augagcacgg cgguccucga gaaccccggc cucggccgca agcucagcga cuucggccag       60

gagacgagcu acaucgagga caacugcaac cagaacggcg cgaucagccu caucuucagc      120

cucaaggagg aggucggcgc gcucgcgaag guccuccgcc ucuucgagga gaacgacguc      180

aaccucacgc acaucgagag ccgccccagc cgccucaaga aggacgagua cgaguucuuc      240

acgcaccucg acaagcgcag ccuccccgcg cucacgaaca ucaucaagau ccuccgccac      300

gacaucggcg cgacggucca cgagcucagc cgcgacaaga agaaggacac gguccccugg      360

uucccccgca cgauccagga gcucgaccgc uucgcgaacc agauccucag cuacggcgcg      420

gagcucgacg cggaccaccc cggcuucaag gaccccgucu accgcgcgcg ccgcaagcag      480

uucgcggaca ucgcguacaa cuaccgccac ggccagccca ucccccgcgu cgaguacaug      540

gaggaggaga agaagacgug gggcacgguc uucaagacgc ucaagagccu cuacaagacg      600

cacgcgugcu acgaguacaa ccacaucuuc ccccuccucg agaaguacug cggcuuccac      660

gaggacaaca ucccccagcu cgaggacguc agccaguucc uccagacgug cacgggcuuc      720

cgccuccgcc ccgucgcggg ccuccucagc agccgcgacu uccucggcgg ccucgcguuc      780

cgcgucuucc acugcacgca guacauccgc cacggcagca agcccaugua cacgcccgag      840

cccgacaucu gccacgagcu ccucggccac gucccccucu ucagcgaccg cagcuucgcg      900

caguucagcc aggagaucgg ccucgcgagc cucggcgcgc ccgacgagua caucgagaag      960

cucgcgacga ucuacugguu cacggucgag uucggccucu gcaagcaggg cgacagcauc     1020

aaggcguacg gcgcgggccu ccucagcagc uucggcgagc uccaguacug ccucagcgag     1080

aagcccaagc uccucccccu cgagcucgag aagacggcga uccagaacua cacggucacg     1140

gaguuccagc cccucuacua cgucgcggag agcuucaacg acgcgaagga gaagguccgc     1200

aacuucgcgg cgacgauccc ccgccccuuc agcguccgcu acgaccccua cacgcagcgc     1260

aucgaggucc ucgacaacac gcagcagcuc aagauccucg cggacagcau caacagcgag     1320

aucggcaucc ucugcagcgc gcuccagaag aucuag                               1356


<210>  143
<211>  441
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: TTR BS_GCU

<400>  143
auggcgagcc accgccuccu ccuccucugc cucgcgggcc ucgucuucgu cagcgaggcg       60

ggccccacgg gcacgggcga gagcaagugc ccccucaugg ucaagguccu cgacgcgguc      120

cgcggcagcc ccgcgaucaa cgucgcgguc cacgucuucc gcaaggcggc ggacgacacg      180

ugggagcccu ucgcgagcgg caagacgagc gagagcggcg agcuccacgg ccucacgacg      240

gaggaggagu ucgucgaggg caucuacaag gucgagaucg acacgaagag cuacuggaag      300

gcgcucggca ucagccccuu ccacgagcac gcggaggucg ucuucacggc gaacgacagc      360

ggcccccgcc gcuacacgau cgcggcgcuc cucagccccu acagcuacag cacgacggcg      420

gucgucacga accccaagua g                                                441


<210>  144

<400>  144
000

<210>  145

<400>  145
000

<210>  146

<400>  146
000

<210>  147

<400>  147
000

<210>  148

<400>  148
000

<210>  149

<400>  149
000

<210>  150

<400>  150
000

<210>  151

<400>  151
000

<210>  152

<400>  152
000

<210>  153

<400>  153
000

<210>  154

<400>  154
000

<210>  155

<400>  155
000

<210>  156

<400>  156
000

<210>  157

<400>  157
000

<210>  158

<400>  158
000

<210>  159

<400>  159
000

<210>  160

<400>  160
000

<210>  161
<211>  1379
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 nickase amino acid sequence

<400>  161

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Gly Gly  Gly Ser Pro Lys Lys  Lys Arg Lys Val 
    1370                 1375                 


<210>  162
<211>  1379
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: dCas9 amino acid sequence

<400>  162

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Gly Gly  Gly Ser Pro Lys Lys  Lys Arg Lys Val 
    1370                 1375                 


<210>  163
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS amino acid sequence

<400>  163

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  164
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS amino acid sequence

<400>  164

Leu Ala Ala Lys Arg Ser Arg Thr Thr 
1               5                   


<210>  165
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS amino acid sequence

<400>  165

Gln Ala Ala Lys Arg Ser Arg Thr Thr 
1               5                   


<210>  166
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS amino acid sequence

<400>  166

Pro Ala Pro Ala Lys Arg Glu Arg Thr Thr 
1               5                   10  


<210>  167
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS amino acid sequence

<400>  167

Gln Ala Ala Lys Arg Pro Arg Thr Thr 
1               5                   


<210>  168
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS amino acid sequence

<400>  168

Arg Ala Ala Lys Arg Pro Arg Thr Thr 
1               5                   


<210>  169
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS amino acid sequence

<400>  169

Ala Ala Ala Lys Arg Ser Trp Ser Met Ala Ala 
1               5                   10      


<210>  170
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS amino acid sequence

<400>  170

Ala Ala Ala Lys Arg Val Trp Ser Met Ala Phe 
1               5                   10      


<210>  171
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS amino acid sequence

<400>  171

Ala Ala Ala Lys Arg Ser Trp Ser Met Ala Phe 
1               5                   10      


<210>  172
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS amino acid sequence

<400>  172

Ala Ala Ala Lys Arg Lys Tyr Phe Ala Ala 
1               5                   10  


<210>  173
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS amino acid sequence

<400>  173

Arg Ala Ala Lys Arg Lys Ala Phe Ala Ala 
1               5                   10  


<210>  174
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS amino acid sequence

<400>  174

Arg Ala Ala Lys Arg Lys Tyr Phe Ala Val 
1               5                   10  


<210>  175
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS amino acid sequence

<400>  175

Pro Lys Lys Lys Arg Arg Val 
1               5           


<210>  176
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary NLS amino acid sequence

<400>  176

Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


<210>  177
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary 5 UTR

<400>  177
acatttgctt ctgacacaac tgtgttcact agcaacctca aacagacacc                  50


<210>  178
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary 5 UTR

<400>  178
cataaaccct ggcgcgctcg cggcccggca ctcttctggt ccccacagac tcagagagaa       60

cccacc                                                                  66


<210>  179
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary 5 UTR

<400>  179
aagctcagaa taaacgctca actttggcc                                         29


<210>  180
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary 5 UTR

<400>  180
cagggtcctg tggacagctc accagct                                           27


<210>  181
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary 5 UTR

<400>  181
tcccgcagtc ggcgtccagc ggctctgctt gttcgtgtgt gtgtcgttgc aggccttatt       60

c                                                                       61


<210>  182
<211>  132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary 3 UTR

<400>  182
gctcgctttc ttgctgtcca atttctatta aaggttcctt tgttccctaa gtccaactac       60

taaactgggg gatattatga agggccttga gcatctggat tctgcctaat aaaaaacatt      120

tattttcatt gc                                                          132


<210>  183
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary 3 UTR

<400>  183
gctggagcct cggtggccat gcttcttgcc ccttgggcct ccccccagcc cctcctcccc       60

ttcctgcacc cgtacccccg tggtctttga ataaagtctg agtgggcggc                 110


<210>  184
<211>  130
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary 3 UTR

<400>  184
accagcctca agaacacccg aatggagtct ctaagctaca taataccaac ttacacttta       60

caaaatgttg tcccccaaaa tgtagccatt cgtatctgct cctaataaaa agaaagtttc      120

ttcacattct                                                             130


<210>  185
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary 3 UTR

<400>  185
ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac       60

tcccactgtc ctttcctaat aaaatgagga aattgcatcg ca                         102


<210>  186
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary 3 UTR

<400>  186
gctgccttct gcggggcttg ccttctggcc atgcccttct tctctccctt gcacctgtac       60

ctcttggtct ttgaataaag cctgagtagg aag                                    93


<210>  187
<211>  13
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary Kozak sequence

<400>  187
gccgccrcca ugg                                                          13


<210>  188
<211>  105
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary poly-A sequence

<400>  188
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gcgaaaaaaa aaaaaaaaaa aaaaaaaaaa       60

aaaccgaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa                      105


<210>  189
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary guide pattern


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (1)..(4)
<223>  PS linkage

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (69)..(100)
<223>  2'-O-Me nucleotide

<220>
<221>  modified_base
<222>  (97)..(100)
<223>  PS linkage

<400>  189
nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  190
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary 5 UTR

<400>  190
cagatcgcct ggagacgcca tccacgctgt tttgacctcc at                          42


<210>  191
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary 5 UTR

<400>  191
agaagacacc gggaccgatc cagcctccgc ggccgggaac gg                          42


<210>  192
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Exemplary 5 UTR

<400>  192
tgcattggaa cgcggattcc ccgtgccaag agtgactcac cg                          42


<210>  193
<211>  4423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ORF32 mRNA

<400>  193
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguaca       60

gcaucggacu ggacaucgga acaaacagcg uuggcugggc ugugaucaca gacgaauaca      120

agguucccuc aaagaaguuc aagguccugg gaaacacaga cagacacagc aucaagaaga      180

aucucaucgg ugcacugcug uucgacagcg gugagacagc agaagccaca agacugaaga      240

gaacagcccg cagaagauac acaagaagaa agaacagaau cugcuaccug caggagaucu      300

ucagcaacga aauggcaaag gucgacgaca gcuucuucca cagacuggaa gaaagcuucc      360

uggucgaaga agacaagaag cacgaaagac acccgaucuu cggaaacauc gucgacgaag      420

ucgcauacca cgaaaaguac cccaccaucu accaccugcg gaagaagcug gucgacucga      480

cugacaaggc agaccugcgg cugaucuacc uggcacuggc acacaugaua aaguucagag      540

gacacuuccu gaucgaagga gaccugaacc cugacaacag cgacgucgac aagcuguuca      600

uccagcuggu ccagaccuac aaccagcugu ucgaagaaaa cccgaucaac gcaagcggag      660

ucgacgcaaa ggcaauccuc agcgcccgcc ucagcaagag cagaagacug gaaaaucuca      720

ucgcacagcu uccaggugag aagaagaaug ggcuguucgg aaaucucauc gcacucagcc      780

ugggacugac ucccaacuuc aagagcaacu ucgaccuggc agaagacgca aagcugcagc      840

ucagcaagga caccuacgac gacgaccugg acaaucuccu ggcacagauc ggagaccagu      900

acgcagaccu guuccuggcu gccaagaauc ucagcgacgc aauccugcuc agcgacaucc      960

ugcgggucaa cacagagauc acaaaggcac cgcucagcgc aagcaugaua aagagauacg     1020

acgaacacca ccaggaccug acacugcuga aggcacuggu cagacagcag cuuccagaga     1080

aguacaagga aaucuucuuc gaccagagca agaaugggua cgccggguac aucgacggug     1140

gugccagcca ggaggaauuc uacaaguuca ucaagccgau ccuggaaaag auggacggaa     1200

cagaggagcu gcuggucaag cugaacaggg aggaccugcu gcggaagcag agaacauucg     1260

acaaugggag caucccccac cagauccacc ugggugagcu gcacgcaauc cugcggagac     1320

aggaggacuu cuacccguuc cugaaggaca acagggagaa gaucgaaaag auccugacau     1380

ucagaauccc cuacuacguu ggcccgcugg cccgcggaaa cagcagauuc gcauggauga     1440

caagaaagag cgaagaaaca aucacucccu ggaacuucga agaagucguc gacaagggug     1500

ccagcgcaca gagcuucauc gaaagaauga caaacuucga caagaaucuu ccaaacgaaa     1560

agguccuucc aaagcacagc cugcuguacg aauacuucac agucuacaac gagcugacaa     1620

aggucaagua cgucacagag ggaaugagaa agccggcauu ccucagcggu gagcagaaga     1680

aggcaaucgu cgaccugcug uucaagacaa acagaaaggu cacagucaag cagcugaagg     1740

aagacuacuu caagaagauc gaaugcuucg acagcgucga aaucagcgga gucgaagaca     1800

gauucaacgc aagccuggga accuaccacg accugcugaa gaucaucaag gacaaggacu     1860

uccuggacaa cgaagaaaac gaagacaucc uggaagacau cguccugaca cugacacugu     1920

ucgaagacag ggagaugaua gaagaaagac ugaagaccua cgcacaccug uucgacgaca     1980

aggucaugaa gcagcugaag agaagaagau acacaggaug gggaagacuc agcagaaagc     2040

ugaucaaugg gauccgagac aagcagagcg gaaagacaau ccuggacuuc cugaagagcg     2100

acggauucgc aaacagaaac uucaugcagc ugauccacga cgacagccug acauucaagg     2160

aagacaucca gaaggcacag gucagcggac agggcgacag ccugcacgaa cacaucgcaa     2220

aucucgccgg gagcccggca aucaagaagg ggauccugca gacagucaag gucgucgacg     2280

agcuggucaa ggucauggga agacacaagc cagagaacau cgucaucgaa auggccaggg     2340

agaaccagac aacucaaaag gggcagaaga acagcaggga gagaaugaag agaaucgaag     2400

aaggaaucaa ggagcuggga agccagaucc ugaaggaaca cccggucgaa aacacucaac     2460

ugcagaacga aaagcuguac cuguacuacc ugcagaaugg gcgagacaug uacgucgacc     2520

aggagcugga caucaacaga cucagcgacu acgacgucga ccacaucguu ccccagagcu     2580

uccugaagga cgacagcauc gacaacaagg uccugacaag aagcgacaag aacagaggaa     2640

agagcgacaa cguucccuca gaagaagucg ucaagaagau gaagaacuac uggagacagc     2700

ugcugaacgc aaagcugauc acucaaagaa aguucgacaa ucucacaaag gcagaaagag     2760

guggccucag cgagcuggac aaggccgggu ucaucaagag acagcugguc gaaacaagac     2820

agaucacaaa gcacgucgca cagauccugg acagcagaau gaacacaaag uacgacgaaa     2880

acgacaagcu gaucagggaa gucaagguca ucacacugaa gagcaagcug gucagcgacu     2940

ucagaaagga cuuccaguuc uacaagguca gggagaucaa caacuaccac cacgcacacg     3000

acgcauaccu gaacgcugug guuggcacag cacugaucaa gaaguacccg aagcuggaaa     3060

gcgaauucgu cuacggagac uacaaggucu acgacgucag aaagaugaua gcaaagagcg     3120

aacaggagau cggaaaggca acagcaaagu acuucuucua cagcaacauc augaacuucu     3180

ucaagacaga gaucacacug gcaaauggug agaucagaaa gagaccgcug aucgaaacaa     3240

auggugagac aggugagauc gucugggaca aggggcgaga cuucgcaaca gucagaaagg     3300

uccucagcau gccgcaggug aacaucguca agaagacaga aguccagaca gguggcuuca     3360

gcaaggaaag cauccuucca aagagaaaca gcgacaagcu gaucgcccgc aagaaggacu     3420

gggacccgaa gaaguacggu ggcuucgaca gccccaccgu cgcauacagc guccuggucg     3480

ucgcaaaggu cgaaaagggg aagagcaaga agcugaagag cgucaaggag cugcugggaa     3540

ucacaaucau ggaaagaagc agcuucgaaa agaacccgau cgacuuccug gaagccaagg     3600

gguacaagga agucaagaag gaccugauca ucaagcuucc aaaguacagc cuguucgagc     3660

uggaaaaugg gagaaagaga augcuggcaa gcgccgguga gcugcagaag gggaacgagc     3720

uggcacuucc cucaaaguac gucaacuucc uguaccuggc aagccacuac gaaaagcuga     3780

aggggagccc agaggacaac gaacagaagc agcuguucgu cgaacagcac aagcacuacc     3840

uggacgaaau caucgaacag aucagcgaau ucagcaagag agucauccug gcagacgcaa     3900

aucucgacaa gguccucagc gcauacaaca agcaccgaga caagccgauc agggagcagg     3960

ccgaaaacau cauccaccug uucacacuga caaaucucgg ugccccggcu gccuucaagu     4020

acuucgacac aacaaucgac agaaagagau acacaucgac uaaggaaguc cuggacgcaa     4080

cacugaucca ccagagcauc acaggacugu acgaaacaag aaucgaccuc agccagcugg     4140

guggcgacgg ugguggcagc ccgaagaaga agagaaaggu cuagcuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucuc     4320

ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaauc uag                       4423


<210>  194
<211>  4423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ORF23 mRNA000112

<400>  194
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguaca       60

gcaucggccu ggacaucggc accaacuccg uuggcugggc ugugaucacc gacgaguaca      120

agguucccuc aaagaaguuc aaggugcugg gcaacaccga ccggcacagc aucaagaaga      180

aucucaucgg ugcacugcug uucgacuccg gugagaccgc cgaagccacc cggcugaagc      240

ggaccgcccg ccggcgguac acccggcgga agaaccggau cugcuaccug caggagaucu      300

ucuccaacga gauggccaag guggacgacu ccuucuucca ccggcuggag gaguccuucc      360

ugguggagga ggacaagaag cacgagcggc accccaucuu cggcaacauc guggacgaag      420

ucgccuacca cgagaaguac cccaccaucu accaccugcg gaagaagcug guggacucga      480

cugacaaggc cgaccugcgg cugaucuacc uggcacuggc ccacaugaua aaguuccggg      540

gccacuuccu gaucgagggc gaccugaacc cugacaacuc cgacguggac aagcuguuca      600

uccagcuggu gcagaccuac aaccagcugu ucgaggagaa ccccaucaac gccuccggcg      660

uggacgccaa ggccauccuc agcgcccgcc ucagcaaguc ccggcggcug gagaaucuca      720

ucgcccagcu uccaggugag aagaagaaug ggcuguucgg caaucucauc gcacucagcc      780

ugggccugac ucccaacuuc aaguccaacu ucgaccuggc cgaggacgcc aagcugcagc      840

ucagcaagga caccuacgac gacgaccugg acaaucuccu ggcccagauc ggcgaccagu      900

acgccgaccu guuccuggcu gccaagaauc ucagcgacgc cauccugcuc agcgacaucc      960

ugcgggugaa cacagagauc accaaggccc cccucagcgc cuccaugaua aagcgguacg     1020

acgagcacca ccaggaccug acccugcuga aggcacuggu gcggcagcag cuuccagaga     1080

aguacaagga gaucuucuuc gaccagagca agaaugggua cgccggguac aucgacggug     1140

gugccuccca ggaggaguuc uacaaguuca ucaagcccau ccuggagaag auggacggca     1200

cagaggagcu gcuggugaag cugaacaggg aggaccugcu gcggaagcag cggaccuucg     1260

acaaugggag caucccccac cagauccacc ugggugagcu gcacgccauc cugcggcggc     1320

aggaggacuu cuaccccuuc cugaaggaca acagggagaa gaucgagaag auccugaccu     1380

uccggauccc cuacuacguu ggcccccugg cccgcggcaa cucccgguuc gccuggauga     1440

cccggaaguc cgaggagacc aucacucccu ggaacuucga ggaagucgug gacaagggug     1500

ccuccgccca gagcuucauc gagcggauga ccaacuucga caagaaucuu ccaaacgaga     1560

aggugcuucc aaagcacucc cugcuguacg aguacuucac cguguacaac gagcugacca     1620

aggugaagua cgugacagag ggcaugcgga agcccgccuu ccucagcggu gagcagaaga     1680

aggccaucgu ggaccugcug uucaagacca accggaaggu gaccgugaag cagcugaagg     1740

aggacuacuu caagaagauc gagugcuucg acuccgugga gaucuccggc guggaggacc     1800

gguucaacgc cucccugggc accuaccacg accugcugaa gaucaucaag gacaaggacu     1860

uccuggacaa cgaggagaac gaggacaucc uggaggacau cgugcugacc cugacccugu     1920

ucgaggacag ggagaugaua gaggagcggc ugaagaccua cgcccaccug uucgacgaca     1980

aggugaugaa gcagcugaag cggcggcggu acaccggcug gggccggcuc agccggaagc     2040

ugaucaaugg gauccgagac aagcagagcg gcaagaccau ccuggacuuc cugaaguccg     2100

acggcuucgc caaccggaac uucaugcagc ugauccacga cgacucccug accuucaagg     2160

aggacaucca gaaggcccag gucagcggcc agggcgacuc ccugcacgag cacaucgcca     2220

aucucgccgg gucccccgcc aucaagaagg ggauccugca gaccgugaag gugguggacg     2280

agcuggugaa ggugaugggc cggcacaagc cagagaacau cgugaucgag auggccaggg     2340

agaaccagac cacucaaaag gggcagaaga acuccaggga gcggaugaag cggaucgagg     2400

agggcaucaa ggagcugggc ucccagaucc ugaaggagca ccccguggag aacacucaac     2460

ugcagaacga gaagcuguac cuguacuacc ugcagaaugg gcgagacaug uacguggacc     2520

aggagcugga caucaaccgg cucagcgacu acgacgugga ccacaucguu ccccagagcu     2580

uccugaagga cgacagcauc gacaacaagg ugcugacccg guccgacaag aaccggggca     2640

aguccgacaa cguucccuca gaggaagucg ugaagaagau gaagaacuac uggcggcagc     2700

ugcugaacgc caagcugauc acucaacgga aguucgacaa ucucaccaag gccgagcggg     2760

guggccucag cgagcuggac aaggccgggu ucaucaagcg gcagcuggug gagacccggc     2820

agaucaccaa gcacguggcc cagauccugg acucccggau gaacaccaag uacgacgaga     2880

acgacaagcu gaucagggaa gucaagguga ucacccugaa guccaagcug gucagcgacu     2940

uccggaagga cuuccaguuc uacaagguga gggagaucaa caacuaccac cacgcccacg     3000

acgccuaccu gaacgcugug guuggcaccg cacugaucaa gaaguacccc aagcuggagu     3060

ccgaguucgu guacggcgac uacaaggugu acgacgugcg gaagaugaua gccaaguccg     3120

agcaggagau cggcaaggcc accgccaagu acuucuucua cuccaacauc augaacuucu     3180

ucaagacaga gaucacccug gccaauggug agauccggaa gcggccccug aucgagacca     3240

auggugagac cggugagauc gugugggaca aggggcgaga cuucgccacc gugcggaagg     3300

ugcucagcau gccccaggug aacaucguga agaagacaga aguccagacc gguggcuucu     3360

ccaaggagag cauccuucca aagcggaacu ccgacaagcu gaucgcccgc aagaaggacu     3420

gggaccccaa gaaguacggu ggcuucgacu cccccaccgu ggccuacucc gugcuggugg     3480

uggccaaggu ggagaagggg aaguccaaga agcugaaguc cgugaaggag cugcugggca     3540

ucaccaucau ggagcggucc uccuucgaga agaaccccau cgacuuccug gaagccaagg     3600

gguacaagga agucaagaag gaccugauca ucaagcuucc aaaguacucc cuguucgagc     3660

uggagaaugg gcggaagcgg augcuggccu ccgccgguga gcugcagaag gggaacgagc     3720

uggcacuucc cucaaaguac gugaacuucc uguaccuggc cucccacuac gagaagcuga     3780

aggggucccc agaggacaac gagcagaagc agcuguucgu ggagcagcac aagcacuacc     3840

uggacgagau caucgagcag aucuccgagu ucuccaagcg ggugauccug gccgacgcca     3900

aucucgacaa ggugcucagc gccuacaaca agcaccgaga caagcccauc agggagcagg     3960

ccgagaacau cauccaccug uucacccuga ccaaucucgg ugcccccgcu gccuucaagu     4020

acuucgacac caccaucgac cggaagcggu acaccucgac uaaggaaguc cuggacgcca     4080

cccugaucca ccagagcauc accggccugu acgagacccg gaucgaccuc agccagcugg     4140

guggcgacgg ugguggcucc cccaagaaga agcggaaggu guagcuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucuc     4320

ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaauc uag                       4423


<210>  195
<211>  4553
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mRNA with SEQ ID No. 67 Cas9 ORF and 3'UTR XBG-XBG

<400>  195
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguacu       60

ccaucggccu ggacaucggc accaacuccg ugggcugggc cgugaucacc gacgaguaca      120

aggugcccuc caagaaguuc aaggugcugg gcaacaccga ccggcacucc aucaagaaga      180

accugaucgg cgcccugcug uucgacuccg gcgagaccgc cgaggccacc cggcugaagc      240

ggaccgcccg gcggcgguac acccggcgga agaaccggau cugcuaccug caggagaucu      300

ucuccaacga gauggccaag guggacgacu ccuucuucca ccggcuggag gaguccuucc      360

ugguggagga ggacaagaag cacgagcggc accccaucuu cggcaacauc guggacgagg      420

uggccuacca cgagaaguac cccaccaucu accaccugcg gaagaagcug guggacucca      480

ccgacaaggc cgaccugcgg cugaucuacc uggcccuggc ccacaugauc aaguuccggg      540

gccacuuccu gaucgagggc gaccugaacc ccgacaacuc cgacguggac aagcuguuca      600

uccagcuggu gcagaccuac aaccagcugu ucgaggagaa ccccaucaac gccuccggcg      660

uggacgccaa ggccauccug uccgcccggc uguccaaguc ccggcggcug gagaaccuga      720

ucgcccagcu gcccggcgag aagaagaacg gccuguucgg caaccugauc gcccuguccc      780

ugggccugac ccccaacuuc aaguccaacu ucgaccuggc cgaggacgcc aagcugcagc      840

uguccaagga caccuacgac gacgaccugg acaaccugcu ggcccagauc ggcgaccagu      900

acgccgaccu guuccuggcc gccaagaacc uguccgacgc cauccugcug uccgacaucc      960

ugcgggugaa caccgagauc accaaggccc cccuguccgc cuccaugauc aagcgguacg     1020

acgagcacca ccaggaccug acccugcuga aggcccuggu gcggcagcag cugcccgaga     1080

aguacaagga gaucuucuuc gaccagucca agaacggcua cgccggcuac aucgacggcg     1140

gcgccuccca ggaggaguuc uacaaguuca ucaagcccau ccuggagaag auggacggca     1200

ccgaggagcu gcuggugaag cugaaccggg aggaccugcu gcggaagcag cggaccuucg     1260

acaacggcuc caucccccac cagauccacc ugggcgagcu gcacgccauc cugcggcggc     1320

aggaggacuu cuaccccuuc cugaaggaca accgggagaa gaucgagaag auccugaccu     1380

uccggauccc cuacuacgug ggcccccugg cccggggcaa cucccgguuc gccuggauga     1440

cccggaaguc cgaggagacc aucacccccu ggaacuucga ggagguggug gacaagggcg     1500

ccuccgccca guccuucauc gagcggauga ccaacuucga caagaaccug cccaacgaga     1560

aggugcugcc caagcacucc cugcuguacg aguacuucac cguguacaac gagcugacca     1620

aggugaagua cgugaccgag ggcaugcgga agcccgccuu ccuguccggc gagcagaaga     1680

aggccaucgu ggaccugcug uucaagacca accggaaggu gaccgugaag cagcugaagg     1740

aggacuacuu caagaagauc gagugcuucg acuccgugga gaucuccggc guggaggacc     1800

gguucaacgc cucccugggc accuaccacg accugcugaa gaucaucaag gacaaggacu     1860

uccuggacaa cgaggagaac gaggacaucc uggaggacau cgugcugacc cugacccugu     1920

ucgaggaccg ggagaugauc gaggagcggc ugaagaccua cgcccaccug uucgacgaca     1980

aggugaugaa gcagcugaag cggcggcggu acaccggcug gggccggcug ucccggaagc     2040

ugaucaacgg cauccgggac aagcaguccg gcaagaccau ccuggacuuc cugaaguccg     2100

acggcuucgc caaccggaac uucaugcagc ugauccacga cgacucccug accuucaagg     2160

aggacaucca gaaggcccag guguccggcc agggcgacuc ccugcacgag cacaucgcca     2220

accuggccgg cucccccgcc aucaagaagg gcauccugca gaccgugaag gugguggacg     2280

agcuggugaa ggugaugggc cggcacaagc ccgagaacau cgugaucgag auggcccggg     2340

agaaccagac cacccagaag ggccagaaga acucccggga gcggaugaag cggaucgagg     2400

agggcaucaa ggagcugggc ucccagaucc ugaaggagca ccccguggag aacacccagc     2460

ugcagaacga gaagcuguac cuguacuacc ugcagaacgg ccgggacaug uacguggacc     2520

aggagcugga caucaaccgg cuguccgacu acgacgugga ccacaucgug ccccaguccu     2580

uccugaagga cgacuccauc gacaacaagg ugcugacccg guccgacaag aaccggggca     2640

aguccgacaa cgugcccucc gaggaggugg ugaagaagau gaagaacuac uggcggcagc     2700

ugcugaacgc caagcugauc acccagcgga aguucgacaa ccugaccaag gccgagcggg     2760

gcggccuguc cgagcuggac aaggccggcu ucaucaagcg gcagcuggug gagacccggc     2820

agaucaccaa gcacguggcc cagauccugg acucccggau gaacaccaag uacgacgaga     2880

acgacaagcu gauccgggag gugaagguga ucacccugaa guccaagcug guguccgacu     2940

uccggaagga cuuccaguuc uacaaggugc gggagaucaa caacuaccac cacgcccacg     3000

acgccuaccu gaacgccgug gugggcaccg cccugaucaa gaaguacccc aagcuggagu     3060

ccgaguucgu guacggcgac uacaaggugu acgacgugcg gaagaugauc gccaaguccg     3120

agcaggagau cggcaaggcc accgccaagu acuucuucua cuccaacauc augaacuucu     3180

ucaagaccga gaucacccug gccaacggcg agauccggaa gcggccccug aucgagacca     3240

acggcgagac cggcgagauc gugugggaca agggccggga cuucgccacc gugcggaagg     3300

ugcuguccau gccccaggug aacaucguga agaagaccga ggugcagacc ggcggcuucu     3360

ccaaggaguc cauccugccc aagcggaacu ccgacaagcu gaucgcccgg aagaaggacu     3420

gggaccccaa gaaguacggc ggcuucgacu cccccaccgu ggccuacucc gugcuggugg     3480

uggccaaggu ggagaagggc aaguccaaga agcugaaguc cgugaaggag cugcugggca     3540

ucaccaucau ggagcggucc uccuucgaga agaaccccau cgacuuccug gaggccaagg     3600

gcuacaagga ggugaagaag gaccugauca ucaagcugcc caaguacucc cuguucgagc     3660

uggagaacgg ccggaagcgg augcuggccu ccgccggcga gcugcagaag ggcaacgagc     3720

uggcccugcc cuccaaguac gugaacuucc uguaccuggc cucccacuac gagaagcuga     3780

agggcucccc cgaggacaac gagcagaagc agcuguucgu ggagcagcac aagcacuacc     3840

uggacgagau caucgagcag aucuccgagu ucuccaagcg ggugauccug gccgacgcca     3900

accuggacaa ggugcugucc gccuacaaca agcaccggga caagcccauc cgggagcagg     3960

ccgagaacau cauccaccug uucacccuga ccaaccuggg cgcccccgcc gccuucaagu     4020

acuucgacac caccaucgac cggaagcggu acaccuccac caaggaggug cuggacgcca     4080

cccugaucca ccaguccauc accggccugu acgagacccg gaucgaccug ucccagcugg     4140

gcggcgacgg cggcggcucc cccaagaaga agcggaaggu gugacuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucua     4320

ccagccucaa gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac     4380

aaaauguugu cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu     4440

ucacauucuc ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaauc uag            4553


<210>  196
<211>  4566
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mRNA with SEQ ID No. 67 Cas9 ORF and 3'UTR AES-mtRNR1

<400>  196
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguacu       60

ccaucggccu ggacaucggc accaacuccg ugggcugggc cgugaucacc gacgaguaca      120

aggugcccuc caagaaguuc aaggugcugg gcaacaccga ccggcacucc aucaagaaga      180

accugaucgg cgcccugcug uucgacuccg gcgagaccgc cgaggccacc cggcugaagc      240

ggaccgcccg gcggcgguac acccggcgga agaaccggau cugcuaccug caggagaucu      300

ucuccaacga gauggccaag guggacgacu ccuucuucca ccggcuggag gaguccuucc      360

ugguggagga ggacaagaag cacgagcggc accccaucuu cggcaacauc guggacgagg      420

uggccuacca cgagaaguac cccaccaucu accaccugcg gaagaagcug guggacucca      480

ccgacaaggc cgaccugcgg cugaucuacc uggcccuggc ccacaugauc aaguuccggg      540

gccacuuccu gaucgagggc gaccugaacc ccgacaacuc cgacguggac aagcuguuca      600

uccagcuggu gcagaccuac aaccagcugu ucgaggagaa ccccaucaac gccuccggcg      660

uggacgccaa ggccauccug uccgcccggc uguccaaguc ccggcggcug gagaaccuga      720

ucgcccagcu gcccggcgag aagaagaacg gccuguucgg caaccugauc gcccuguccc      780

ugggccugac ccccaacuuc aaguccaacu ucgaccuggc cgaggacgcc aagcugcagc      840

uguccaagga caccuacgac gacgaccugg acaaccugcu ggcccagauc ggcgaccagu      900

acgccgaccu guuccuggcc gccaagaacc uguccgacgc cauccugcug uccgacaucc      960

ugcgggugaa caccgagauc accaaggccc cccuguccgc cuccaugauc aagcgguacg     1020

acgagcacca ccaggaccug acccugcuga aggcccuggu gcggcagcag cugcccgaga     1080

aguacaagga gaucuucuuc gaccagucca agaacggcua cgccggcuac aucgacggcg     1140

gcgccuccca ggaggaguuc uacaaguuca ucaagcccau ccuggagaag auggacggca     1200

ccgaggagcu gcuggugaag cugaaccggg aggaccugcu gcggaagcag cggaccuucg     1260

acaacggcuc caucccccac cagauccacc ugggcgagcu gcacgccauc cugcggcggc     1320

aggaggacuu cuaccccuuc cugaaggaca accgggagaa gaucgagaag auccugaccu     1380

uccggauccc cuacuacgug ggcccccugg cccggggcaa cucccgguuc gccuggauga     1440

cccggaaguc cgaggagacc aucacccccu ggaacuucga ggagguggug gacaagggcg     1500

ccuccgccca guccuucauc gagcggauga ccaacuucga caagaaccug cccaacgaga     1560

aggugcugcc caagcacucc cugcuguacg aguacuucac cguguacaac gagcugacca     1620

aggugaagua cgugaccgag ggcaugcgga agcccgccuu ccuguccggc gagcagaaga     1680

aggccaucgu ggaccugcug uucaagacca accggaaggu gaccgugaag cagcugaagg     1740

aggacuacuu caagaagauc gagugcuucg acuccgugga gaucuccggc guggaggacc     1800

gguucaacgc cucccugggc accuaccacg accugcugaa gaucaucaag gacaaggacu     1860

uccuggacaa cgaggagaac gaggacaucc uggaggacau cgugcugacc cugacccugu     1920

ucgaggaccg ggagaugauc gaggagcggc ugaagaccua cgcccaccug uucgacgaca     1980

aggugaugaa gcagcugaag cggcggcggu acaccggcug gggccggcug ucccggaagc     2040

ugaucaacgg cauccgggac aagcaguccg gcaagaccau ccuggacuuc cugaaguccg     2100

acggcuucgc caaccggaac uucaugcagc ugauccacga cgacucccug accuucaagg     2160

aggacaucca gaaggcccag guguccggcc agggcgacuc ccugcacgag cacaucgcca     2220

accuggccgg cucccccgcc aucaagaagg gcauccugca gaccgugaag gugguggacg     2280

agcuggugaa ggugaugggc cggcacaagc ccgagaacau cgugaucgag auggcccggg     2340

agaaccagac cacccagaag ggccagaaga acucccggga gcggaugaag cggaucgagg     2400

agggcaucaa ggagcugggc ucccagaucc ugaaggagca ccccguggag aacacccagc     2460

ugcagaacga gaagcuguac cuguacuacc ugcagaacgg ccgggacaug uacguggacc     2520

aggagcugga caucaaccgg cuguccgacu acgacgugga ccacaucgug ccccaguccu     2580

uccugaagga cgacuccauc gacaacaagg ugcugacccg guccgacaag aaccggggca     2640

aguccgacaa cgugcccucc gaggaggugg ugaagaagau gaagaacuac uggcggcagc     2700

ugcugaacgc caagcugauc acccagcgga aguucgacaa ccugaccaag gccgagcggg     2760

gcggccuguc cgagcuggac aaggccggcu ucaucaagcg gcagcuggug gagacccggc     2820

agaucaccaa gcacguggcc cagauccugg acucccggau gaacaccaag uacgacgaga     2880

acgacaagcu gauccgggag gugaagguga ucacccugaa guccaagcug guguccgacu     2940

uccggaagga cuuccaguuc uacaaggugc gggagaucaa caacuaccac cacgcccacg     3000

acgccuaccu gaacgccgug gugggcaccg cccugaucaa gaaguacccc aagcuggagu     3060

ccgaguucgu guacggcgac uacaaggugu acgacgugcg gaagaugauc gccaaguccg     3120

agcaggagau cggcaaggcc accgccaagu acuucuucua cuccaacauc augaacuucu     3180

ucaagaccga gaucacccug gccaacggcg agauccggaa gcggccccug aucgagacca     3240

acggcgagac cggcgagauc gugugggaca agggccggga cuucgccacc gugcggaagg     3300

ugcuguccau gccccaggug aacaucguga agaagaccga ggugcagacc ggcggcuucu     3360

ccaaggaguc cauccugccc aagcggaacu ccgacaagcu gaucgcccgg aagaaggacu     3420

gggaccccaa gaaguacggc ggcuucgacu cccccaccgu ggccuacucc gugcuggugg     3480

uggccaaggu ggagaagggc aaguccaaga agcugaaguc cgugaaggag cugcugggca     3540

ucaccaucau ggagcggucc uccuucgaga agaaccccau cgacuuccug gaggccaagg     3600

gcuacaagga ggugaagaag gaccugauca ucaagcugcc caaguacucc cuguucgagc     3660

uggagaacgg ccggaagcgg augcuggccu ccgccggcga gcugcagaag ggcaacgagc     3720

uggcccugcc cuccaaguac gugaacuucc uguaccuggc cucccacuac gagaagcuga     3780

agggcucccc cgaggacaac gagcagaagc agcuguucgu ggagcagcac aagcacuacc     3840

uggacgagau caucgagcag aucuccgagu ucuccaagcg ggugauccug gccgacgcca     3900

accuggacaa ggugcugucc gccuacaaca agcaccggga caagcccauc cgggagcagg     3960

ccgagaacau cauccaccug uucacccuga ccaaccuggg cgcccccgcc gccuucaagu     4020

acuucgacac caccaucgac cggaagcggu acaccuccac caaggaggug cuggacgcca     4080

cccugaucca ccaguccauc accggccugu acgagacccg gaucgaccug ucccagcugg     4140

gcggcgacgg cggcggcucc cccaagaaga agcggaaggu gugacuggua cugcaugcac     4200

gcaaugcuag cugccccuuu cccguccugg guaccccgag ucucccccga ccucgggucc     4260

cagguaugcu cccaccucca ccugccccac ucaccaccuc ugcuaguucc agacaccucc     4320

caagcacgca gcaaugcagc ucaaaacgcu uagccuagcc acacccccac gggaaacagc     4380

agugauuaac cuuuagcaau aaacgaaagu uuaacuaagc uauacuaacc ccaggguugg     4440

ucaauuucgu gccagccaca cccucgagaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aucuag                                                                4566


<210>  197
<211>  4566
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mRNA with SEQ ID No. 67 Cas9 ORF and 3'UTR mtRNR1-AES

<400>  197
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguacu       60

ccaucggccu ggacaucggc accaacuccg ugggcugggc cgugaucacc gacgaguaca      120

aggugcccuc caagaaguuc aaggugcugg gcaacaccga ccggcacucc aucaagaaga      180

accugaucgg cgcccugcug uucgacuccg gcgagaccgc cgaggccacc cggcugaagc      240

ggaccgcccg gcggcgguac acccggcgga agaaccggau cugcuaccug caggagaucu      300

ucuccaacga gauggccaag guggacgacu ccuucuucca ccggcuggag gaguccuucc      360

ugguggagga ggacaagaag cacgagcggc accccaucuu cggcaacauc guggacgagg      420

uggccuacca cgagaaguac cccaccaucu accaccugcg gaagaagcug guggacucca      480

ccgacaaggc cgaccugcgg cugaucuacc uggcccuggc ccacaugauc aaguuccggg      540

gccacuuccu gaucgagggc gaccugaacc ccgacaacuc cgacguggac aagcuguuca      600

uccagcuggu gcagaccuac aaccagcugu ucgaggagaa ccccaucaac gccuccggcg      660

uggacgccaa ggccauccug uccgcccggc uguccaaguc ccggcggcug gagaaccuga      720

ucgcccagcu gcccggcgag aagaagaacg gccuguucgg caaccugauc gcccuguccc      780

ugggccugac ccccaacuuc aaguccaacu ucgaccuggc cgaggacgcc aagcugcagc      840

uguccaagga caccuacgac gacgaccugg acaaccugcu ggcccagauc ggcgaccagu      900

acgccgaccu guuccuggcc gccaagaacc uguccgacgc cauccugcug uccgacaucc      960

ugcgggugaa caccgagauc accaaggccc cccuguccgc cuccaugauc aagcgguacg     1020

acgagcacca ccaggaccug acccugcuga aggcccuggu gcggcagcag cugcccgaga     1080

aguacaagga gaucuucuuc gaccagucca agaacggcua cgccggcuac aucgacggcg     1140

gcgccuccca ggaggaguuc uacaaguuca ucaagcccau ccuggagaag auggacggca     1200

ccgaggagcu gcuggugaag cugaaccggg aggaccugcu gcggaagcag cggaccuucg     1260

acaacggcuc caucccccac cagauccacc ugggcgagcu gcacgccauc cugcggcggc     1320

aggaggacuu cuaccccuuc cugaaggaca accgggagaa gaucgagaag auccugaccu     1380

uccggauccc cuacuacgug ggcccccugg cccggggcaa cucccgguuc gccuggauga     1440

cccggaaguc cgaggagacc aucacccccu ggaacuucga ggagguggug gacaagggcg     1500

ccuccgccca guccuucauc gagcggauga ccaacuucga caagaaccug cccaacgaga     1560

aggugcugcc caagcacucc cugcuguacg aguacuucac cguguacaac gagcugacca     1620

aggugaagua cgugaccgag ggcaugcgga agcccgccuu ccuguccggc gagcagaaga     1680

aggccaucgu ggaccugcug uucaagacca accggaaggu gaccgugaag cagcugaagg     1740

aggacuacuu caagaagauc gagugcuucg acuccgugga gaucuccggc guggaggacc     1800

gguucaacgc cucccugggc accuaccacg accugcugaa gaucaucaag gacaaggacu     1860

uccuggacaa cgaggagaac gaggacaucc uggaggacau cgugcugacc cugacccugu     1920

ucgaggaccg ggagaugauc gaggagcggc ugaagaccua cgcccaccug uucgacgaca     1980

aggugaugaa gcagcugaag cggcggcggu acaccggcug gggccggcug ucccggaagc     2040

ugaucaacgg cauccgggac aagcaguccg gcaagaccau ccuggacuuc cugaaguccg     2100

acggcuucgc caaccggaac uucaugcagc ugauccacga cgacucccug accuucaagg     2160

aggacaucca gaaggcccag guguccggcc agggcgacuc ccugcacgag cacaucgcca     2220

accuggccgg cucccccgcc aucaagaagg gcauccugca gaccgugaag gugguggacg     2280

agcuggugaa ggugaugggc cggcacaagc ccgagaacau cgugaucgag auggcccggg     2340

agaaccagac cacccagaag ggccagaaga acucccggga gcggaugaag cggaucgagg     2400

agggcaucaa ggagcugggc ucccagaucc ugaaggagca ccccguggag aacacccagc     2460

ugcagaacga gaagcuguac cuguacuacc ugcagaacgg ccgggacaug uacguggacc     2520

aggagcugga caucaaccgg cuguccgacu acgacgugga ccacaucgug ccccaguccu     2580

uccugaagga cgacuccauc gacaacaagg ugcugacccg guccgacaag aaccggggca     2640

aguccgacaa cgugcccucc gaggaggugg ugaagaagau gaagaacuac uggcggcagc     2700

ugcugaacgc caagcugauc acccagcgga aguucgacaa ccugaccaag gccgagcggg     2760

gcggccuguc cgagcuggac aaggccggcu ucaucaagcg gcagcuggug gagacccggc     2820

agaucaccaa gcacguggcc cagauccugg acucccggau gaacaccaag uacgacgaga     2880

acgacaagcu gauccgggag gugaagguga ucacccugaa guccaagcug guguccgacu     2940

uccggaagga cuuccaguuc uacaaggugc gggagaucaa caacuaccac cacgcccacg     3000

acgccuaccu gaacgccgug gugggcaccg cccugaucaa gaaguacccc aagcuggagu     3060

ccgaguucgu guacggcgac uacaaggugu acgacgugcg gaagaugauc gccaaguccg     3120

agcaggagau cggcaaggcc accgccaagu acuucuucua cuccaacauc augaacuucu     3180

ucaagaccga gaucacccug gccaacggcg agauccggaa gcggccccug aucgagacca     3240

acggcgagac cggcgagauc gugugggaca agggccggga cuucgccacc gugcggaagg     3300

ugcuguccau gccccaggug aacaucguga agaagaccga ggugcagacc ggcggcuucu     3360

ccaaggaguc cauccugccc aagcggaacu ccgacaagcu gaucgcccgg aagaaggacu     3420

gggaccccaa gaaguacggc ggcuucgacu cccccaccgu ggccuacucc gugcuggugg     3480

uggccaaggu ggagaagggc aaguccaaga agcugaaguc cgugaaggag cugcugggca     3540

ucaccaucau ggagcggucc uccuucgaga agaaccccau cgacuuccug gaggccaagg     3600

gcuacaagga ggugaagaag gaccugauca ucaagcugcc caaguacucc cuguucgagc     3660

uggagaacgg ccggaagcgg augcuggccu ccgccggcga gcugcagaag ggcaacgagc     3720

uggcccugcc cuccaaguac gugaacuucc uguaccuggc cucccacuac gagaagcuga     3780

agggcucccc cgaggacaac gagcagaagc agcuguucgu ggagcagcac aagcacuacc     3840

uggacgagau caucgagcag aucuccgagu ucuccaagcg ggugauccug gccgacgcca     3900

accuggacaa ggugcugucc gccuacaaca agcaccggga caagcccauc cgggagcagg     3960

ccgagaacau cauccaccug uucacccuga ccaaccuggg cgcccccgcc gccuucaagu     4020

acuucgacac caccaucgac cggaagcggu acaccuccac caaggaggug cuggacgcca     4080

cccugaucca ccaguccauc accggccugu acgagacccg gaucgaccug ucccagcugg     4140

gcggcgacgg cggcggcucc cccaagaaga agcggaaggu gugacaagca cgcagcaaug     4200

cagcucaaaa cgcuuagccu agccacaccc ccacgggaaa cagcagugau uaaccuuuag     4260

caauaaacga aaguuuaacu aagcuauacu aaccccaggg uuggucaauu ucgugccagc     4320

cacacccugg uacugcaugc acgcaaugcu agcugccccu uucccguccu ggguaccccg     4380

agucuccccc gaccucgggu cccagguaug cucccaccuc caccugcccc acucaccacc     4440

ucugcuaguu ccagacaccu cccucgagaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aucuag                                                                4566


<210>  198

<400>  198
000

<210>  199
<211>  4560
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mRNA with SEQ ID No. 46 Cas9 ORF and 3'UTR XBG-XBG

<400>  199
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguaca       60

gcaucggccu ggacaucggc accaacuccg uuggcugggc ugugaucacc gacgaguaca      120

agguucccuc aaagaaguuc aaggugcugg gcaacaccga ccggcacagc aucaagaaga      180

aucucaucgg ugcacugcug uucgacuccg gugagaccgc cgaagccacc cggcugaagc      240

ggaccgcccg ccggcgguac acccggcgga agaaccggau cugcuaccug caggagaucu      300

ucuccaacga gauggccaag guggacgacu ccuucuucca ccggcuggag gaguccuucc      360

ugguggagga ggacaagaag cacgagcggc accccaucuu cggcaacauc guggacgaag      420

ucgccuacca cgagaaguac cccaccaucu accaccugcg gaagaagcug guggacucga      480

cugacaaggc cgaccugcgg cugaucuacc uggcacuggc ccacaugaua aaguuccggg      540

gccacuuccu gaucgagggc gaccugaacc cugacaacuc cgacguggac aagcuguuca      600

uccagcuggu gcagaccuac aaccagcugu ucgaggagaa ccccaucaac gccuccggcg      660

uggacgccaa ggccauccuc agcgcccgcc ucagcaaguc ccggcggcug gagaaucuca      720

ucgcccagcu uccaggugag aagaagaaug ggcuguucgg caaucucauc gcacucagcc      780

ugggccugac ucccaacuuc aaguccaacu ucgaccuggc cgaggacgcc aagcugcagc      840

ucagcaagga caccuacgac gacgaccugg acaaucuccu ggcccagauc ggcgaccagu      900

acgccgaccu guuccuggcu gccaagaauc ucagcgacgc cauccugcuc agcgacaucc      960

ugcgggugaa cacagagauc accaaggccc cccucagcgc cuccaugaua aagcgguacg     1020

acgagcacca ccaggaccug acccugcuga aggcacuggu gcggcagcag cuuccagaga     1080

aguacaagga gaucuucuuc gaccagagca agaaugggua cgccggguac aucgacggug     1140

gugccuccca ggaggaguuc uacaaguuca ucaagcccau ccuggagaag auggacggca     1200

cagaggagcu gcuggugaag cugaacaggg aggaccugcu gcggaagcag cggaccuucg     1260

acaaugggag caucccccac cagauccacc ugggugagcu gcacgccauc cugcggcggc     1320

aggaggacuu cuaccccuuc cugaaggaca acagggagaa gaucgagaag auccugaccu     1380

uccggauccc cuacuacguu ggcccccugg cccgcggcaa cucccgguuc gccuggauga     1440

cccggaaguc cgaggagacc aucacucccu ggaacuucga ggaagucgug gacaagggug     1500

ccuccgccca gagcuucauc gagcggauga ccaacuucga caagaaucuu ccaaacgaga     1560

aggugcuucc aaagcacucc cugcuguacg aguacuucac cguguacaac gagcugacca     1620

aggugaagua cgugacagag ggcaugcgga agcccgccuu ccucagcggu gagcagaaga     1680

aggccaucgu ggaccugcug uucaagacca accggaaggu gaccgugaag cagcugaagg     1740

aggacuacuu caagaagauc gagugcuucg acuccgugga gaucuccggc guggaggacc     1800

gguucaacgc cucccugggc accuaccacg accugcugaa gaucaucaag gacaaggacu     1860

uccuggacaa cgaggagaac gaggacaucc uggaggacau cgugcugacc cugacccugu     1920

ucgaggacag ggagaugaua gaggagcggc ugaagaccua cgcccaccug uucgacgaca     1980

aggugaugaa gcagcugaag cggcggcggu acaccggcug gggccggcuc agccggaagc     2040

ugaucaaugg gauccgagac aagcagagcg gcaagaccau ccuggacuuc cugaaguccg     2100

acggcuucgc caaccggaac uucaugcagc ugauccacga cgacucccug accuucaagg     2160

aggacaucca gaaggcccag gucagcggcc agggcgacuc ccugcacgag cacaucgcca     2220

aucucgccgg gucccccgcc aucaagaagg ggauccugca gaccgugaag gugguggacg     2280

agcuggugaa ggugaugggc cggcacaagc cagagaacau cgugaucgag auggccaggg     2340

agaaccagac cacucaaaag gggcagaaga acuccaggga gcggaugaag cggaucgagg     2400

agggcaucaa ggagcugggc ucccagaucc ugaaggagca ccccguggag aacacucaac     2460

ugcagaacga gaagcuguac cuguacuacc ugcagaaugg gcgagacaug uacguggacc     2520

aggagcugga caucaaccgg cucagcgacu acgacgugga ccacaucguu ccccagagcu     2580

uccugaagga cgacagcauc gacaacaagg ugcugacccg guccgacaag aaccggggca     2640

aguccgacaa cguucccuca gaggaagucg ugaagaagau gaagaacuac uggcggcagc     2700

ugcugaacgc caagcugauc acucaacgga aguucgacaa ucucaccaag gccgagcggg     2760

guggccucag cgagcuggac aaggccgggu ucaucaagcg gcagcuggug gagacccggc     2820

agaucaccaa gcacguggcc cagauccugg acucccggau gaacaccaag uacgacgaga     2880

acgacaagcu gaucagggaa gucaagguga ucacccugaa guccaagcug gucagcgacu     2940

uccggaagga cuuccaguuc uacaagguga gggagaucaa caacuaccac cacgcccacg     3000

acgccuaccu gaacgcugug guuggcaccg cacugaucaa gaaguacccc aagcuggagu     3060

ccgaguucgu guacggcgac uacaaggugu acgacgugcg gaagaugaua gccaaguccg     3120

agcaggagau cggcaaggcc accgccaagu acuucuucua cuccaacauc augaacuucu     3180

ucaagacaga gaucacccug gccaauggug agauccggaa gcggccccug aucgagacca     3240

auggugagac cggugagauc gugugggaca aggggcgaga cuucgccacc gugcggaagg     3300

ugcucagcau gccccaggug aacaucguga agaagacaga aguccagacc gguggcuucu     3360

ccaaggagag cauccuucca aagcggaacu ccgacaagcu gaucgcccgc aagaaggacu     3420

gggaccccaa gaaguacggu ggcuucgacu cccccaccgu ggccuacucc gugcuggugg     3480

uggccaaggu ggagaagggg aaguccaaga agcugaaguc cgugaaggag cugcugggca     3540

ucaccaucau ggagcggucc uccuucgaga agaaccccau cgacuuccug gaagccaagg     3600

gguacaagga agucaagaag gaccugauca ucaagcuucc aaaguacucc cuguucgagc     3660

uggagaaugg gcggaagcgg augcuggccu ccgccgguga gcugcagaag gggaacgagc     3720

uggcacuucc cucaaaguac gugaacuucc uguaccuggc cucccacuac gagaagcuga     3780

aggggucccc agaggacaac gagcagaagc agcuguucgu ggagcagcac aagcacuacc     3840

uggacgagau caucgagcag aucuccgagu ucuccaagcg ggugauccug gccgacgcca     3900

aucucgacaa ggugcucagc gccuacaaca agcaccgaga caagcccauc agggagcagg     3960

ccgagaacau cauccaccug uucacccuga ccaaucucgg ugcccccgcu gccuucaagu     4020

acuucgacac caccaucgac cggaagcggu acaccucgac uaaggaaguc cuggacgcca     4080

cccugaucca ccagagcauc accggccugu acgagacccg gaucgaccuc agccagcugg     4140

guggcgacgg ugguggcucc cccaagaaga agcggaaggu guagcuagca ccagccucaa     4200

gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac aaaauguugu     4260

cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu ucacauucua     4320

ccagccucaa gaacacccga auggagucuc uaagcuacau aauaccaacu uacacuuuac     4380

aaaauguugu cccccaaaau guagccauuc guaucugcuc cuaauaaaaa gaaaguuucu     4440

ucacauucuc ucgagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaucuag     4560


<210>  200
<211>  4573
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mRNA with SEQ ID No. 46 Cas9 ORF and 3'UTR AES-mtRNR1

<400>  200
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguaca       60

gcaucggccu ggacaucggc accaacuccg uuggcugggc ugugaucacc gacgaguaca      120

agguucccuc aaagaaguuc aaggugcugg gcaacaccga ccggcacagc aucaagaaga      180

aucucaucgg ugcacugcug uucgacuccg gugagaccgc cgaagccacc cggcugaagc      240

ggaccgcccg ccggcgguac acccggcgga agaaccggau cugcuaccug caggagaucu      300

ucuccaacga gauggccaag guggacgacu ccuucuucca ccggcuggag gaguccuucc      360

ugguggagga ggacaagaag cacgagcggc accccaucuu cggcaacauc guggacgaag      420

ucgccuacca cgagaaguac cccaccaucu accaccugcg gaagaagcug guggacucga      480

cugacaaggc cgaccugcgg cugaucuacc uggcacuggc ccacaugaua aaguuccggg      540

gccacuuccu gaucgagggc gaccugaacc cugacaacuc cgacguggac aagcuguuca      600

uccagcuggu gcagaccuac aaccagcugu ucgaggagaa ccccaucaac gccuccggcg      660

uggacgccaa ggccauccuc agcgcccgcc ucagcaaguc ccggcggcug gagaaucuca      720

ucgcccagcu uccaggugag aagaagaaug ggcuguucgg caaucucauc gcacucagcc      780

ugggccugac ucccaacuuc aaguccaacu ucgaccuggc cgaggacgcc aagcugcagc      840

ucagcaagga caccuacgac gacgaccugg acaaucuccu ggcccagauc ggcgaccagu      900

acgccgaccu guuccuggcu gccaagaauc ucagcgacgc cauccugcuc agcgacaucc      960

ugcgggugaa cacagagauc accaaggccc cccucagcgc cuccaugaua aagcgguacg     1020

acgagcacca ccaggaccug acccugcuga aggcacuggu gcggcagcag cuuccagaga     1080

aguacaagga gaucuucuuc gaccagagca agaaugggua cgccggguac aucgacggug     1140

gugccuccca ggaggaguuc uacaaguuca ucaagcccau ccuggagaag auggacggca     1200

cagaggagcu gcuggugaag cugaacaggg aggaccugcu gcggaagcag cggaccuucg     1260

acaaugggag caucccccac cagauccacc ugggugagcu gcacgccauc cugcggcggc     1320

aggaggacuu cuaccccuuc cugaaggaca acagggagaa gaucgagaag auccugaccu     1380

uccggauccc cuacuacguu ggcccccugg cccgcggcaa cucccgguuc gccuggauga     1440

cccggaaguc cgaggagacc aucacucccu ggaacuucga ggaagucgug gacaagggug     1500

ccuccgccca gagcuucauc gagcggauga ccaacuucga caagaaucuu ccaaacgaga     1560

aggugcuucc aaagcacucc cugcuguacg aguacuucac cguguacaac gagcugacca     1620

aggugaagua cgugacagag ggcaugcgga agcccgccuu ccucagcggu gagcagaaga     1680

aggccaucgu ggaccugcug uucaagacca accggaaggu gaccgugaag cagcugaagg     1740

aggacuacuu caagaagauc gagugcuucg acuccgugga gaucuccggc guggaggacc     1800

gguucaacgc cucccugggc accuaccacg accugcugaa gaucaucaag gacaaggacu     1860

uccuggacaa cgaggagaac gaggacaucc uggaggacau cgugcugacc cugacccugu     1920

ucgaggacag ggagaugaua gaggagcggc ugaagaccua cgcccaccug uucgacgaca     1980

aggugaugaa gcagcugaag cggcggcggu acaccggcug gggccggcuc agccggaagc     2040

ugaucaaugg gauccgagac aagcagagcg gcaagaccau ccuggacuuc cugaaguccg     2100

acggcuucgc caaccggaac uucaugcagc ugauccacga cgacucccug accuucaagg     2160

aggacaucca gaaggcccag gucagcggcc agggcgacuc ccugcacgag cacaucgcca     2220

aucucgccgg gucccccgcc aucaagaagg ggauccugca gaccgugaag gugguggacg     2280

agcuggugaa ggugaugggc cggcacaagc cagagaacau cgugaucgag auggccaggg     2340

agaaccagac cacucaaaag gggcagaaga acuccaggga gcggaugaag cggaucgagg     2400

agggcaucaa ggagcugggc ucccagaucc ugaaggagca ccccguggag aacacucaac     2460

ugcagaacga gaagcuguac cuguacuacc ugcagaaugg gcgagacaug uacguggacc     2520

aggagcugga caucaaccgg cucagcgacu acgacgugga ccacaucguu ccccagagcu     2580

uccugaagga cgacagcauc gacaacaagg ugcugacccg guccgacaag aaccggggca     2640

aguccgacaa cguucccuca gaggaagucg ugaagaagau gaagaacuac uggcggcagc     2700

ugcugaacgc caagcugauc acucaacgga aguucgacaa ucucaccaag gccgagcggg     2760

guggccucag cgagcuggac aaggccgggu ucaucaagcg gcagcuggug gagacccggc     2820

agaucaccaa gcacguggcc cagauccugg acucccggau gaacaccaag uacgacgaga     2880

acgacaagcu gaucagggaa gucaagguga ucacccugaa guccaagcug gucagcgacu     2940

uccggaagga cuuccaguuc uacaagguga gggagaucaa caacuaccac cacgcccacg     3000

acgccuaccu gaacgcugug guuggcaccg cacugaucaa gaaguacccc aagcuggagu     3060

ccgaguucgu guacggcgac uacaaggugu acgacgugcg gaagaugaua gccaaguccg     3120

agcaggagau cggcaaggcc accgccaagu acuucuucua cuccaacauc augaacuucu     3180

ucaagacaga gaucacccug gccaauggug agauccggaa gcggccccug aucgagacca     3240

auggugagac cggugagauc gugugggaca aggggcgaga cuucgccacc gugcggaagg     3300

ugcucagcau gccccaggug aacaucguga agaagacaga aguccagacc gguggcuucu     3360

ccaaggagag cauccuucca aagcggaacu ccgacaagcu gaucgcccgc aagaaggacu     3420

gggaccccaa gaaguacggu ggcuucgacu cccccaccgu ggccuacucc gugcuggugg     3480

uggccaaggu ggagaagggg aaguccaaga agcugaaguc cgugaaggag cugcugggca     3540

ucaccaucau ggagcggucc uccuucgaga agaaccccau cgacuuccug gaagccaagg     3600

gguacaagga agucaagaag gaccugauca ucaagcuucc aaaguacucc cuguucgagc     3660

uggagaaugg gcggaagcgg augcuggccu ccgccgguga gcugcagaag gggaacgagc     3720

uggcacuucc cucaaaguac gugaacuucc uguaccuggc cucccacuac gagaagcuga     3780

aggggucccc agaggacaac gagcagaagc agcuguucgu ggagcagcac aagcacuacc     3840

uggacgagau caucgagcag aucuccgagu ucuccaagcg ggugauccug gccgacgcca     3900

aucucgacaa ggugcucagc gccuacaaca agcaccgaga caagcccauc agggagcagg     3960

ccgagaacau cauccaccug uucacccuga ccaaucucgg ugcccccgcu gccuucaagu     4020

acuucgacac caccaucgac cggaagcggu acaccucgac uaaggaaguc cuggacgcca     4080

cccugaucca ccagagcauc accggccugu acgagacccg gaucgaccuc agccagcugg     4140

guggcgacgg ugguggcucc cccaagaaga agcggaaggu guagcuggua cugcaugcac     4200

gcaaugcuag cugccccuuu cccguccugg guaccccgag ucucccccga ccucgggucc     4260

cagguaugcu cccaccucca ccugccccac ucaccaccuc ugcuaguucc agacaccucc     4320

caagcacgca gcaaugcagc ucaaaacgcu uagccuagcc acacccccac gggaaacagc     4380

agugauuaac cuuuagcaau aaacgaaagu uuaacuaagc uauacuaacc ccaggguugg     4440

ucaauuucgu gccagccaca cccucgagaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaauc uag                                                        4573


<210>  201
<211>  4573
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mRNA with SEQ ID No. 46 Cas9 ORF and 3'UTR mtRNR1-AES

<400>  201
gggaagcuca gaauaaacgc ucaacuuugg ccggaucugc caccauggac aagaaguaca       60

gcaucggccu ggacaucggc accaacuccg uuggcugggc ugugaucacc gacgaguaca      120

agguucccuc aaagaaguuc aaggugcugg gcaacaccga ccggcacagc aucaagaaga      180

aucucaucgg ugcacugcug uucgacuccg gugagaccgc cgaagccacc cggcugaagc      240

ggaccgcccg ccggcgguac acccggcgga agaaccggau cugcuaccug caggagaucu      300

ucuccaacga gauggccaag guggacgacu ccuucuucca ccggcuggag gaguccuucc      360

ugguggagga ggacaagaag cacgagcggc accccaucuu cggcaacauc guggacgaag      420

ucgccuacca cgagaaguac cccaccaucu accaccugcg gaagaagcug guggacucga      480

cugacaaggc cgaccugcgg cugaucuacc uggcacuggc ccacaugaua aaguuccggg      540

gccacuuccu gaucgagggc gaccugaacc cugacaacuc cgacguggac aagcuguuca      600

uccagcuggu gcagaccuac aaccagcugu ucgaggagaa ccccaucaac gccuccggcg      660

uggacgccaa ggccauccuc agcgcccgcc ucagcaaguc ccggcggcug gagaaucuca      720

ucgcccagcu uccaggugag aagaagaaug ggcuguucgg caaucucauc gcacucagcc      780

ugggccugac ucccaacuuc aaguccaacu ucgaccuggc cgaggacgcc aagcugcagc      840

ucagcaagga caccuacgac gacgaccugg acaaucuccu ggcccagauc ggcgaccagu      900

acgccgaccu guuccuggcu gccaagaauc ucagcgacgc cauccugcuc agcgacaucc      960

ugcgggugaa cacagagauc accaaggccc cccucagcgc cuccaugaua aagcgguacg     1020

acgagcacca ccaggaccug acccugcuga aggcacuggu gcggcagcag cuuccagaga     1080

aguacaagga gaucuucuuc gaccagagca agaaugggua cgccggguac aucgacggug     1140

gugccuccca ggaggaguuc uacaaguuca ucaagcccau ccuggagaag auggacggca     1200

cagaggagcu gcuggugaag cugaacaggg aggaccugcu gcggaagcag cggaccuucg     1260

acaaugggag caucccccac cagauccacc ugggugagcu gcacgccauc cugcggcggc     1320

aggaggacuu cuaccccuuc cugaaggaca acagggagaa gaucgagaag auccugaccu     1380

uccggauccc cuacuacguu ggcccccugg cccgcggcaa cucccgguuc gccuggauga     1440

cccggaaguc cgaggagacc aucacucccu ggaacuucga ggaagucgug gacaagggug     1500

ccuccgccca gagcuucauc gagcggauga ccaacuucga caagaaucuu ccaaacgaga     1560

aggugcuucc aaagcacucc cugcuguacg aguacuucac cguguacaac gagcugacca     1620

aggugaagua cgugacagag ggcaugcgga agcccgccuu ccucagcggu gagcagaaga     1680

aggccaucgu ggaccugcug uucaagacca accggaaggu gaccgugaag cagcugaagg     1740

aggacuacuu caagaagauc gagugcuucg acuccgugga gaucuccggc guggaggacc     1800

gguucaacgc cucccugggc accuaccacg accugcugaa gaucaucaag gacaaggacu     1860

uccuggacaa cgaggagaac gaggacaucc uggaggacau cgugcugacc cugacccugu     1920

ucgaggacag ggagaugaua gaggagcggc ugaagaccua cgcccaccug uucgacgaca     1980

aggugaugaa gcagcugaag cggcggcggu acaccggcug gggccggcuc agccggaagc     2040

ugaucaaugg gauccgagac aagcagagcg gcaagaccau ccuggacuuc cugaaguccg     2100

acggcuucgc caaccggaac uucaugcagc ugauccacga cgacucccug accuucaagg     2160

aggacaucca gaaggcccag gucagcggcc agggcgacuc ccugcacgag cacaucgcca     2220

aucucgccgg gucccccgcc aucaagaagg ggauccugca gaccgugaag gugguggacg     2280

agcuggugaa ggugaugggc cggcacaagc cagagaacau cgugaucgag auggccaggg     2340

agaaccagac cacucaaaag gggcagaaga acuccaggga gcggaugaag cggaucgagg     2400

agggcaucaa ggagcugggc ucccagaucc ugaaggagca ccccguggag aacacucaac     2460

ugcagaacga gaagcuguac cuguacuacc ugcagaaugg gcgagacaug uacguggacc     2520

aggagcugga caucaaccgg cucagcgacu acgacgugga ccacaucguu ccccagagcu     2580

uccugaagga cgacagcauc gacaacaagg ugcugacccg guccgacaag aaccggggca     2640

aguccgacaa cguucccuca gaggaagucg ugaagaagau gaagaacuac uggcggcagc     2700

ugcugaacgc caagcugauc acucaacgga aguucgacaa ucucaccaag gccgagcggg     2760

guggccucag cgagcuggac aaggccgggu ucaucaagcg gcagcuggug gagacccggc     2820

agaucaccaa gcacguggcc cagauccugg acucccggau gaacaccaag uacgacgaga     2880

acgacaagcu gaucagggaa gucaagguga ucacccugaa guccaagcug gucagcgacu     2940

uccggaagga cuuccaguuc uacaagguga gggagaucaa caacuaccac cacgcccacg     3000

acgccuaccu gaacgcugug guuggcaccg cacugaucaa gaaguacccc aagcuggagu     3060

ccgaguucgu guacggcgac uacaaggugu acgacgugcg gaagaugaua gccaaguccg     3120

agcaggagau cggcaaggcc accgccaagu acuucuucua cuccaacauc augaacuucu     3180

ucaagacaga gaucacccug gccaauggug agauccggaa gcggccccug aucgagacca     3240

auggugagac cggugagauc gugugggaca aggggcgaga cuucgccacc gugcggaagg     3300

ugcucagcau gccccaggug aacaucguga agaagacaga aguccagacc gguggcuucu     3360

ccaaggagag cauccuucca aagcggaacu ccgacaagcu gaucgcccgc aagaaggacu     3420

gggaccccaa gaaguacggu ggcuucgacu cccccaccgu ggccuacucc gugcuggugg     3480

uggccaaggu ggagaagggg aaguccaaga agcugaaguc cgugaaggag cugcugggca     3540

ucaccaucau ggagcggucc uccuucgaga agaaccccau cgacuuccug gaagccaagg     3600

gguacaagga agucaagaag gaccugauca ucaagcuucc aaaguacucc cuguucgagc     3660

uggagaaugg gcggaagcgg augcuggccu ccgccgguga gcugcagaag gggaacgagc     3720

uggcacuucc cucaaaguac gugaacuucc uguaccuggc cucccacuac gagaagcuga     3780

aggggucccc agaggacaac gagcagaagc agcuguucgu ggagcagcac aagcacuacc     3840

uggacgagau caucgagcag aucuccgagu ucuccaagcg ggugauccug gccgacgcca     3900

aucucgacaa ggugcucagc gccuacaaca agcaccgaga caagcccauc agggagcagg     3960

ccgagaacau cauccaccug uucacccuga ccaaucucgg ugcccccgcu gccuucaagu     4020

acuucgacac caccaucgac cggaagcggu acaccucgac uaaggaaguc cuggacgcca     4080

cccugaucca ccagagcauc accggccugu acgagacccg gaucgaccuc agccagcugg     4140

guggcgacgg ugguggcucc cccaagaaga agcggaaggu guagcaagca cgcagcaaug     4200

cagcucaaaa cgcuuagccu agccacaccc ccacgggaaa cagcagugau uaaccuuuag     4260

caauaaacga aaguuuaacu aagcuauacu aaccccaggg uuggucaauu ucgugccagc     4320

cacacccugg uacugcaugc acgcaaugcu agcugccccu uucccguccu ggguaccccg     4380

agucuccccc gaccucgggu cccagguaug cucccaccuc caccugcccc acucaccacc     4440

ucugcuaguu ccagacaccu cccucgagaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaauc uag                                                        4573


<210>  202
<211>  278
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' AES-mtRNR1 UTR

<400>  202
ctggtactgc atgcacgcaa tgctagctgc ccctttcccg tcctgggtac cccgagtctc       60

ccccgacctc gggtcccagg tatgctccca cctccacctg ccccactcac cacctctgct      120

agttccagac acctcccaag cacgcagcaa tgcagctcaa aacgcttagc ctagccacac      180

ccccacggga aacagcagtg attaaccttt agcaataaac gaaagtttaa ctaagctata      240

ctaaccccag ggttggtcaa tttcgtgcca gccacacc                              278


<210>  203
<211>  278
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' mtRNR1-AES UTR

<400>  203
caagcacgca gcaatgcagc tcaaaacgct tagcctagcc acacccccac gggaaacagc       60

agtgattaac ctttagcaat aaacgaaagt ttaactaagc tatactaacc ccagggttgg      120

tcaatttcgt gccagccaca ccctggtact gcatgcacgc aatgctagct gcccctttcc      180

cgtcctgggt accccgagtc tcccccgacc tcgggtccca ggtatgctcc cacctccacc      240

tgccccactc accacctctg ctagttccag acacctcc                              278


<210>  204
<211>  260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' XBG-XBG UTR

<400>  204
accagcctca agaacacccg aatggagtct ctaagctaca taataccaac ttacacttta       60

caaaatgttg tcccccaaaa tgtagccatt cgtatctgct cctaataaaa agaaagtttc      120

ttcacattct accagcctca agaacacccg aatggagtct ctaagctaca taataccaac      180

ttacacttta caaaatgttg tcccccaaaa tgtagccatt cgtatctgct cctaataaaa      240

agaaagtttc ttcacattct                                                  260


