                         SEQUENCE LISTING

<110>  Trustees of the University of Pennsylvania
       Zhao, Yangbing
       Pan, Wei
       Liu, Xiaojun
 
<120>  DNMT3A Knockout CAR T Cells For Adoptive Immunotherapy

<130>  046483-7247WO1(02248)

<140>  U.S. Provisional Patent Application No. 62/824,018
<141>  2019-03-26

<160>  129   

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  X2

<400>  1
ctcgtcatcg cctgctttgg                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  X5

<400>  2
tcaggcgtgg tagccacagt                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  X7

<400>  3
tggctcgtca tcgcctgctt                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  X10

<400>  4
ctaccacgcc tgagcccgtg                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  X14

<400>  5
gacaagaatg ccaccaaagc                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  4

<400>  6
cgatgacgag ccagagtacg                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  10

<400>  7
aagccgctca cctcgtactc                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  30

<400>  8
gctaccacgc ctgagcccgt                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  31

<400>  9
gagcccgtgg ggtccgatgc                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  34

<400>  10
ggctaccacg cctgagcccg                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  7.1

<400>  11
ggggcccggg gagtctcaga                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  7.2

<400>  12
gcccgtgggg tccgatgctg                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  15original

<400>  13
tgtcttggtg gatgacgggc                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  S1

<400>  14
tctgagactc cccgggcccc                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  S2

<400>  15
ctcgtcatcg cctgctttgg                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  S3

<400>  16
caggcgtggt agccacagtg                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  S4

<400>  17
ggaagaaaac caggggcccg                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  X3

<400>  18
tccgaaccac atgacccagc                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  X4

<400>  19
cggagacggc aaattctcag                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  32

<400>  20
gacggccggg gctttggcat                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  33

<400>  21
ggccaggccg cattgtgtct                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  35

<400>  22
tgggtcatgt ggttcggaga                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  37

<400>  23
tgggtcatgt ggttcggaga                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  8.1

<400>  24
cggccggggc tttggcattg                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  8.2

<400>  25
tgggtcatgt ggttcggaga                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  8.3

<400>  26
acccgctggg tcatgtggtt                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  16original

<400>  27
tccccagcat cggaccccac                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  9

<400>  28
catgggctgc ttgttgtacg                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  12

<400>  29
gctgcttgtt gtacgtggcc                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  15

<400>  30
gcactgcaaa acgagctcag                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  25

<400>  31
gttttgcagt gcgttccacc                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  27

<400>  32
gcttgttgta cgtggcctgg                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  ADD1

<400>  33
agaacaagcc catgattgaa                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  13

<400>  34
catcgctgtc gtggcacacc                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  14

<400>  35
ccgggaacag cttccccgcg                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  24

<400>  36
cccagggccc attcaatcat                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  ADD3

<400>  37
aaagccccgg aagagcacag                                                   20


<210>  38
<211>  19
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  ADD12

<400>  38
agaagtgtac acggacatg                                                    19


<210>  39
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  3

<400>  39
attattgatg agcgcacaag                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  ADD2

<400>  40
aagaagtgta cacggacatg                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  26

<400>  41
ttctccgctg tgctcttccg                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  ADD9

<400>  42
agcggctggt gtacgaggtg                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  ADD10

<400>  43
acgaggtgcg gcagaagtgc                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  18

<400>  44
tgcagagcgg ctggtgtacg                                                   20


<210>  45
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  29

<400>  45
cagaagtgcc ggaacattga                                                   20


<210>  46
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  16

<400>  46
ggcacattcc tccaacgaag                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  28

<400>  47
gcacattcct ccaacgaaga                                                   20


<210>  48
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  1

<400>  48
gcgtaccagt acgacgacga                                                   20


<210>  49
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  5

<400>  49
ggtagccgtc gtcgtcgtac                                                   20


<210>  50
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  8

<400>  50
gcggaaacaa caactgctgc                                                   20


<210>  51
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  2

<400>  51
cttcgctaat aaccacgacc                                                   20


<210>  52
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  6

<400>  52
tgcgggcaca agggtaccta                                                   20


<210>  53
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  11

<400>  53
tgctacatgt gcgggcacaa                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  17

<400>  54
ccgcacatgt agcagttcca                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  19

<400>  55
gcgggcacaa gggtacctac                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  21

<400>  56
cactcacaaa ttcctggtcg                                                   20


<210>  57
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  22

<400>  57
ggttattagc gaagaacatc                                                   20


<210>  58
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  23

<400>  58
gtacctacgg gctgctgcgg                                                   20


<210>  59
<211>  17
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  ADD11

<400>  59
gcatgatgcg cggccca                                                      17


<210>  60
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  ADD1

<400>  60
agaacaagcc catgattgaa                                                   20


<210>  61
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  ADD2

<400>  61
aagaagtgta cacggacatg                                                   20


<210>  62
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  ADD3

<400>  62
aaagccccgg aagagcacag                                                   20


<210>  63
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  ADD9

<400>  63
agcggctggt gtacgaggtg                                                   20


<210>  64
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  ADD10

<400>  64
acgaggtgcg gcagaagtgc                                                   20


<210>  65
<211>  17
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  ADD11

<400>  65
gcatgatgcg cggccca                                                      17


<210>  66
<211>  19
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  ADD12

<400>  66
agaagtgtac acggacatg                                                    19


<210>  67
<211>  24
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  EGFP 5-1

<400>  67
cttctgtcac tgttccgggt tttg                                              24


<210>  68
<211>  23
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  EGFP 5-2

<400>  68
gccactccca ctgtcctttc cta                                               23


<210>  69
<211>  22
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  EGFP 3-1

<400>  69
cacaagggta gcggcgaaga tc                                                22


<210>  70
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  EGFP 3-2

<400>  70
acatgcccag aagcggtgga                                                   20


<210>  71
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon7 For

<400>  71
tttccatttt tcacggcaag                                                   20


<210>  72
<211>  19
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon7 Rev

<400>  72
caccccaatt ccagactgc                                                    19


<210>  73
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon7 Seq

<400>  73
ggagctccat ctgaatgagg                                                   20


<210>  74
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon8 For

<400>  74
ttttgctctg tcttgcctca                                                   20


<210>  75
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon8 Rev

<400>  75
acttccaggc ctcctagtgc                                                   20


<210>  76
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon8 Seq

<400>  76
ttttgctctg tcttgcctca                                                   20


<210>  77
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon9-10 For

<400>  77
actgtatctg gtcccctcca                                                   20


<210>  78
<211>  21
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon9-10 Rev

<400>  78
ccaacagaga gcaggtcatt c                                                 21


<210>  79
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon9-10 Seq1

<400>  79
actgtatctg gtcccctcca                                                   20


<210>  80
<211>  21
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon9-10 Seq2

<400>  80
ccaacagaga gcaggtcatt c                                                 21


<210>  81
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon11-12 For

<400>  81
ctggggtcag gacttgaatg                                                   20


<210>  82
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon11-12 Rev

<400>  82
ccattgacag gagagcagaa                                                   20


<210>  83
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon11-12 Seq1

<400>  83
ctggggtcag gacttgaatg                                                   20


<210>  84
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon11-12 Seq2

<400>  84
ccattgacag gagagcagaa                                                   20


<210>  85
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon13 For

<400>  85
agatgatggc gttcgagact                                                   20


<210>  86
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon13 Rev

<400>  86
caaaagcttg aaacccaagg                                                   20


<210>  87
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon13 Seq

<400>  87
caaaagcttg aaacccaagg                                                   20


<210>  88
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon14-15 For

<400>  88
ctgaccctgg ctaaggtggt                                                   20


<210>  89
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon14-15 Rev

<400>  89
agggtcctaa gcagtgagca                                                   20


<210>  90
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon14-15 Seq1

<400>  90
ctgaccctgg ctaaggtggt                                                   20


<210>  91
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon14-15 Seq2

<400>  91
agggtcctaa gcagtgagca                                                   20


<210>  92
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon19 For

<400>  92
gacagctatt cccgatgacc                                                   20


<210>  93
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon19 Rev

<400>  93
tgcaaagcag aagtcaccag                                                   20


<210>  94
<211>  20
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Primer

<220>
<223>  Exon19 Seq

<400>  94
tgcaaagcag aagtcaccag                                                   20


<210>  95
<211>  9
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Epitope

<220>
<223>  NY-ESO157-165

<400>  95

Ser Leu Leu Met Trp Ile Thr Gln Cys 
1               5                   


<210>  96
<211>  5
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Linker


<220>
<221>  REPEAT
<222>  (1)..(5)
<223>  repeat n times, where n represents an integer of at least 1

<400>  96

Gly Ser Gly Gly Ser 
1               5   


<210>  97
<211>  4
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Linker


<220>
<221>  REPEAT
<222>  (1)..(4)
<223>  repeat n times, where n represents an integer of at least 1

<400>  97

Gly Gly Gly Ser 
1               


<210>  98
<211>  5
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Linker

<400>  98

Gly Gly Gly Gly Ser 
1               5   


<210>  99
<211>  4
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Linker

<400>  99

Gly Gly Ser Gly 
1               


<210>  100
<211>  5
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Linker

<400>  100

Gly Gly Ser Gly Gly 
1               5   


<210>  101
<211>  5
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Linker

<400>  101

Gly Ser Gly Ser Gly 
1               5   


<210>  102
<211>  5
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Linker

<400>  102

Gly Ser Gly Gly Gly 
1               5   


<210>  103
<211>  5
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Linker

<400>  103

Gly Gly Gly Ser Gly 
1               5   


<210>  104
<211>  5
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Linker

<400>  104

Gly Ser Ser Ser Gly 
1               5   


<210>  105
<211>  5
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Linker

<400>  105

Gly Gly Gly Gly Ser 
1               5   


<210>  106
<211>  15
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Linker

<400>  106

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  107
<211>  45
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Linker

<400>  107
ggtggcggtg gctcgggcgg tggtgggtcg ggtggcggcg gatct                       45


<210>  108
<211>  5
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Hinge

<400>  108

Asp Lys Thr His Thr 
1               5   


<210>  109
<211>  4
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Hinge

<400>  109

Cys Pro Pro Cys 
1               


<210>  110
<211>  15
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Hinge

<400>  110

Cys Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg 
1               5                   10                  15  


<210>  111
<211>  12
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Hinge

<400>  111

Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr 
1               5                   10          


<210>  112
<211>  10
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Hinge

<400>  112

Lys Ser Cys Asp Lys Thr His Thr Cys Pro 
1               5                   10  


<210>  113
<211>  7
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Hinge


<400>  113

Lys Cys Cys Val Asp Cys Pro 
1               5           


<210>  114
<211>  7
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Hinge

<400>  114

Lys Tyr Gly Pro Pro Cys Pro 
1               5           


<210>  115
<211>  15
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Hinge

<220>
<223>  Hu IgG1

<400>  115

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  116
<211>  12
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Hinge

<220>
<223>  Hu IgG2

<400>  116

Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro 
1               5                   10          


<210>  117
<211>  17
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Hinge

<220>
<223>  Hu IgG3

<400>  117

Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Cys Pro Arg Cys 
1               5                   10                  15      


Pro 
    


<210>  118
<211>  12
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Hinge

<220>
<223>  Hu IgG4

<400>  118

Ser Pro Asn Met Val Pro His Ala His His Ala Gln 
1               5                   10          


<210>  119
<211>  15
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Hinge

<220>
<223>  Hu IgG1 H229Y

<400>  119

Glu Pro Lys Ser Cys Asp Lys Thr Tyr Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  120
<211>  4
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Furin Cleavage

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<400>  120

Arg Xaa Lys Arg 
1               


<210>  121
<211>  4
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Furin Cleavage

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<400>  121

Arg Xaa Arg Arg 
1               


<210>  122
<211>  5
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Furin Cleavage

<220>
<221>  Xaa
<222>  (1)..(1)
<223>  Xaa is K or R

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  Xaa
<222>  (4)..(4)
<223>  Xaa is K or R

<400>  122

Xaa Arg Xaa Xaa Arg 
1               5   


<210>  123
<211>  4
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Furin Cleavage

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  123

Arg Xaa Xaa Arg 
1               


<210>  124
<211>  4
<212>  PRT
<213>  Artifical Sequence

<220>
<223>  Furin Cleavage


<400>  124

Arg Gly Lys Arg 
1               


<210>  125
<211>  34
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  gRNA

<220>
<223>  "8-2, 3, 4 Target"

<400>  125
aaggcacccg ctgggtcatg tggttcggag acgg                                   34


<210>  126
<211>  835
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Donor Sequence

<220>
<223>  5' Homologous Arm

<400>  126
aaggcacccg ctgggtcatg tggttcggag acggacattg aggctcccac aggagatgca       60

gatgtctgga aagcagaggg aggggatggg gtgagagtgc cagagttccc aggcaacaaa      120

cttaccctca atgttccggc acttctgccg cacctcgtac accagccgct ctgcaagggg      180

aggagagctg gcgtcagagg tgccaccctc tccagaagca ggccaactac ctcttgtgcg      240

ctcatcaata atctccttga ccttgggctt ctccgctgtg ctcttccggg gctttttggc      300

tggtggaggt ggtgcgtagg cagctgcctc aggttccacc cacatgtccg tgtacacttc      360

tttgtaggga ttcttctctt ctggaggagg aaagcaggtg ccaaggtcag ggtcccagaa      420

agctgggtgc cctcatttac cttctggtgg ctccaggccc ttagggccag aaggctggaa      480

gccccccagg gcccattcaa tcatgggctt gttctgcacc tccacggcct tggcagtgtc      540

actctcatcg ctgtcgtggc acaccgggaa cagcttcccc gcgcggctgc tggccacctg      600

gagggtgaca cgccagggtt ggggttgctc ctccgagctc ccagcaggga cactcacctg      660

caggacctcg tagatggctt tgcggtacat gggctgcttg ttgtacgtgg cctggtggaa      720

cgcactgcaa aacgagctca gcggcatcag cttctcaaca cacacctggg gggacaagcc      780

aggccttgtt tgccgcccag gctactgcca aaccccacaa cttaccactg agaat           835


<210>  127
<211>  2082
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Donor Sequence

<220>
<223>  hPGK promoter-eGFP-WPRE-BghPolyA

<400>  127
ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc       60

tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc      120

cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc      180

tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac      240

ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc      300

gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag      360

cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct      420

gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct      480

cgttgaccga atcaccgacc tctctcccca gggggatcat cgaattacct ctagagccac      540

catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga      600

cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta      660

cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac      720

cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa      780

gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt      840

cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct      900

ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca      960

caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa     1020

cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc     1080

cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca     1140

ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt     1200

cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta     1260

agtcgacatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat     1320

gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct     1380

tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag     1440

gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc     1500

cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc     1560

ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct     1620

cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccttgg     1680

ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg     1740

gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg     1800

cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgct     1860

gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg     1920

gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg     1980

agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg     2040

gaagacaata gcaggcatgc tggggatgcg gtgggctcta tg                        2082


<210>  128
<211>  834
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Donor Sequence

<220>
<223>  3' Homologous Arm

<400>  128
gacccagcgg gtgccttcag ctgctcggct ccggcccgtc atccaccaag acacaatgcg       60

gcctggccac caggagaagc cccgcagttt cccccacacc agctccccaa tgccaaagcc      120

ccggccgtcc tggagcccca aggagcagaa atcattacac tggccacggc tggtgaagaa      180

gccgctcacc tcgtactctg gctcgtcatc gcctgctttg gtggcattct tgtccccagc      240

atcggacccc acgggctcag gcgtggtagc cacagtgggg gatgcggggt cagtgggctg      300

ctgcacagca ggagggctgg cctcctccac cttctgagac tccccgggcc cctggttttc      360

ttccacagca ttcattcctg caatgacctt ggctttcttc tcagcctggg gaaacaaaaa      420

acaaaaagtc accttggctg gggcccaggc cagaaggcgc ctcacctccc ttttccagcg      480

tgccagccac tcgtcccgct tgcgcttgct gatgtagtag gggtcccccg cctggaaggt      540

gagcctcggc atgggccgct gacggaggct ggactcccag cccaagccac cccgcagccg      600

gccccgggag ccctaggaca gagagacaga cattagggca ttccacagag cccctggggg      660

tggaacactt gcctccattt tcatggattc gatgttggtc tccttctgtt ctttgcctgt      720

ggagagggaa gaacaaaggg accagtaaga ggctgcccct ggtgctgagg actcacccgc      780

ttctgcaggg gctcctcggc ccgtctccga accacatgac ccagcgggtg cctt            834


<210>  129
<211>  3759
<212>  DNA
<213>  Artifical Sequence

<220>
<223>  Donor Sequence

<220>
<223>  Complete

<400>  129
aaggcacccg ctgggtcatg tggttcggag acggacattg aggctcccac aggagatgca       60

gatgtctgga aagcagaggg aggggatggg gtgagagtgc cagagttccc aggcaacaaa      120

cttaccctca atgttccggc acttctgccg cacctcgtac accagccgct ctgcaagggg      180

aggagagctg gcgtcagagg tgccaccctc tccagaagca ggccaactac ctcttgtgcg      240

ctcatcaata atctccttga ccttgggctt ctccgctgtg ctcttccggg gctttttggc      300

tggtggaggt ggtgcgtagg cagctgcctc aggttccacc cacatgtccg tgtacacttc      360

tttgtaggga ttcttctctt ctggaggagg aaagcaggtg ccaaggtcag ggtcccagaa      420

agctgggtgc cctcatttac cttctggtgg ctccaggccc ttagggccag aaggctggaa      480

gccccccagg gcccattcaa tcatgggctt gttctgcacc tccacggcct tggcagtgtc      540

actctcatcg ctgtcgtggc acaccgggaa cagcttcccc gcgcggctgc tggccacctg      600

gagggtgaca cgccagggtt ggggttgctc ctccgagctc ccagcaggga cactcacctg      660

caggacctcg tagatggctt tgcggtacat gggctgcttg ttgtacgtgg cctggtggaa      720

cgcactgcaa aacgagctca gcggcatcag cttctcaaca cacacctggg gggacaagcc      780

aggccttgtt tgccgcccag gctactgcca aaccccacaa cttaccactg agaatgcgat      840

cgcggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg      900

ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac      960

gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct     1020

tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca     1080

aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc     1140

agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag     1200

cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt     1260

cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc     1320

cctcgttgac cgaatcaccg acctctctcc ccagggggat catcgaatta cctctagagc     1380

caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct     1440

ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac     1500

ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc     1560

caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat     1620

gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat     1680

cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac     1740

cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg     1800

gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa     1860

gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct     1920

cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa     1980

ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat     2040

ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa     2100

gtaagtcgac atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac     2160

tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt     2220

gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat     2280

gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca     2340

acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc     2400

cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg     2460

gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcct     2520

tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct     2580

tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt     2640

ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct     2700

gctgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc     2760

ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt     2820

ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat     2880

tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggaccc agcgggtgcc     2940

ttcagctgct cggctccggc ccgtcatcca ccaagacaca atgcggcctg gccaccagga     3000

gaagccccgc agtttccccc acaccagctc cccaatgcca aagccccggc cgtcctggag     3060

ccccaaggag cagaaatcat tacactggcc acggctggtg aagaagccgc tcacctcgta     3120

ctctggctcg tcatcgcctg ctttggtggc attcttgtcc ccagcatcgg accccacggg     3180

ctcaggcgtg gtagccacag tgggggatgc ggggtcagtg ggctgctgca cagcaggagg     3240

gctggcctcc tccaccttct gagactcccc gggcccctgg ttttcttcca cagcattcat     3300

tcctgcaatg accttggctt tcttctcagc ctggggaaac aaaaaacaaa aagtcacctt     3360

ggctggggcc caggccagaa ggcgcctcac ctcccttttc cagcgtgcca gccactcgtc     3420

ccgcttgcgc ttgctgatgt agtaggggtc ccccgcctgg aaggtgagcc tcggcatggg     3480

ccgctgacgg aggctggact cccagcccaa gccaccccgc agccggcccc gggagcccta     3540

ggacagagag acagacatta gggcattcca cagagcccct gggggtggaa cacttgcctc     3600

cattttcatg gattcgatgt tggtctcctt ctgttctttg cctgtggaga gggaagaaca     3660

aagggaccag taagaggctg cccctggtgc tgaggactca cccgcttctg caggggctcc     3720

tcggcccgtc tccgaaccac atgacccagc gggtgcctt                            3759


