                         SEQUENCE LISTING

<110>  SAIPEM S.P.A.
       
 
<120>  CARBONIC ANHYDRASE VARIANTS FOR IMPROVED CO2 CAPTURE

<130>  @E0125174

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wild-type TACA sequence with N terminus modified for improved 
       bacterial expression

<400>  1

Met Glu His Glu Trp Gly Tyr Ser Gly Ser Ile Gly Pro Glu His Trp 
1               5                   10                  15      


Gly Asp Leu Ser Pro Glu Tyr Leu Met Cys Lys Ile Gly Lys Asn Gln 
            20                  25                  30          


Ser Pro Ile Asp Ile Asn Ser Ala Asp Ala Val Lys Ala Cys Leu Ala 
        35                  40                  45              


Pro Val Ser Val Tyr Tyr Val Ser Asp Ala Lys Tyr Val Val Asn Asn 
    50                  55                  60                  


Gly His Thr Ile Lys Val Val Met Gly Gly Arg Gly Tyr Val Val Val 
65                  70                  75                  80  


Asp Gly Lys Arg Phe Tyr Leu Lys Gln Phe His Phe His Ala Pro Ser 
                85                  90                  95      


Glu His Thr Val Asn Gly Lys His Tyr Pro Phe Glu Ala His Phe Val 
            100                 105                 110         


His Leu Asp Lys Asn Gly Asn Ile Thr Val Leu Gly Val Phe Phe Lys 
        115                 120                 125             


Val Gly Lys Glu Asn Pro Glu Leu Glu Lys Val Trp Arg Val Met Pro 
    130                 135                 140                 


Glu Glu Pro Gly Gln Lys Arg His Leu Thr Ala Arg Ile Asp Pro Glu 
145                 150                 155                 160 


Lys Leu Leu Pro Glu Asn Arg Asp Tyr Tyr Arg Tyr Ser Gly Ser Leu 
                165                 170                 175     


Thr Thr Pro Pro Cys Ser Glu Gly Val Arg Trp Ile Val Phe Lys Glu 
            180                 185                 190         


Pro Val Glu Met Ser Arg Glu Gln Leu Glu Lys Phe Arg Lys Val Met 
        195                 200                 205             


Gly Phe Asp Asn Asn Arg Pro Val Gln Pro Leu Asn Ala Arg Lys Val 
    210                 215                 220                 


Met Lys 
225     


<210>  2
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 1 + R156E

<400>  2

Met Glu His Glu Trp Gly Tyr Ser Gly Ser Ile Gly Pro Glu His Trp 
1               5                   10                  15      


Gly Asp Leu Ser Pro Glu Tyr Leu Met Cys Lys Ile Gly Lys Asn Gln 
            20                  25                  30          


Ser Pro Ile Asp Ile Asn Ser Ala Asp Ala Val Lys Ala Cys Leu Ala 
        35                  40                  45              


Pro Val Ser Val Tyr Tyr Val Ser Asp Ala Lys Tyr Val Val Asn Asn 
    50                  55                  60                  


Gly His Thr Ile Lys Val Val Met Gly Gly Arg Gly Tyr Val Val Val 
65                  70                  75                  80  


Asp Gly Lys Arg Phe Tyr Leu Lys Gln Phe His Phe His Ala Pro Ser 
                85                  90                  95      


Glu His Thr Val Asn Gly Lys His Tyr Pro Phe Glu Ala His Phe Val 
            100                 105                 110         


His Leu Asp Lys Asn Gly Asn Ile Thr Val Leu Gly Val Phe Phe Lys 
        115                 120                 125             


Val Gly Lys Glu Asn Pro Glu Leu Glu Lys Val Trp Arg Val Met Pro 
    130                 135                 140                 


Glu Glu Pro Gly Gln Lys Arg His Leu Thr Ala Glu Ile Asp Pro Glu 
145                 150                 155                 160 


Lys Leu Leu Pro Glu Asn Arg Asp Tyr Tyr Arg Tyr Ser Gly Ser Leu 
                165                 170                 175     


Thr Thr Pro Pro Cys Ser Glu Gly Val Arg Trp Ile Val Phe Lys Glu 
            180                 185                 190         


Pro Val Glu Met Ser Arg Glu Gln Leu Glu Lys Phe Arg Lys Val Met 
        195                 200                 205             


Gly Phe Asp Asn Asn Arg Pro Val Gln Pro Leu Asn Ala Arg Lys Val 
    210                 215                 220                 


Met Lys 
225     


<210>  3
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 1 + K27R, N38D, K88R, K116R, N119D, K128R, R156E, 
       E160D, D168E, E192D, E199D, K203R, K206R, V216T, L219I

<400>  3

Met Glu His Glu Trp Gly Tyr Ser Gly Ser Ile Gly Pro Glu His Trp 
1               5                   10                  15      


Gly Asp Leu Ser Pro Glu Tyr Leu Met Cys Arg Ile Gly Lys Asn Gln 
            20                  25                  30          


Ser Pro Ile Asp Ile Asp Ser Ala Asp Ala Val Lys Ala Cys Leu Ala 
        35                  40                  45              


Pro Val Ser Val Tyr Tyr Val Ser Asp Ala Lys Tyr Val Val Asn Asn 
    50                  55                  60                  


Gly His Thr Ile Lys Val Val Met Gly Gly Arg Gly Tyr Val Val Val 
65                  70                  75                  80  


Asp Gly Lys Arg Phe Tyr Leu Arg Gln Phe His Phe His Ala Pro Ser 
                85                  90                  95      


Glu His Thr Val Asn Gly Lys His Tyr Pro Phe Glu Ala His Phe Val 
            100                 105                 110         


His Leu Asp Arg Asn Gly Asp Ile Thr Val Leu Gly Val Phe Phe Arg 
        115                 120                 125             


Val Gly Lys Glu Asn Pro Glu Leu Glu Lys Val Trp Arg Val Met Pro 
    130                 135                 140                 


Glu Glu Pro Gly Gln Lys Arg His Leu Thr Ala Glu Ile Asp Pro Asp 
145                 150                 155                 160 


Lys Leu Leu Pro Glu Asn Arg Glu Tyr Tyr Arg Tyr Ser Gly Ser Leu 
                165                 170                 175     


Thr Thr Pro Pro Cys Ser Glu Gly Val Arg Trp Ile Val Phe Lys Asp 
            180                 185                 190         


Pro Val Glu Met Ser Arg Asp Gln Leu Glu Arg Phe Arg Arg Val Met 
        195                 200                 205             


Gly Phe Asp Asn Asn Arg Pro Thr Gln Pro Ile Asn Ala Arg Lys Val 
    210                 215                 220                 


Met Lys 
225     


<210>  4
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 1 + Y77F, V79E, K88E, Y105F, K116E, K128E, E137D, 
       E145D, R156E, D168E, Y170F, E195D, E199D, V216T, L219I, K226R

<400>  4

Met Glu His Glu Trp Gly Tyr Ser Gly Ser Ile Gly Pro Glu His Trp 
1               5                   10                  15      


Gly Asp Leu Ser Pro Glu Tyr Leu Met Cys Lys Ile Gly Lys Asn Gln 
            20                  25                  30          


Ser Pro Ile Asp Ile Asn Ser Ala Asp Ala Val Lys Ala Cys Leu Ala 
        35                  40                  45              


Pro Val Ser Val Tyr Tyr Val Ser Asp Ala Lys Tyr Val Val Asn Asn 
    50                  55                  60                  


Gly His Thr Ile Lys Val Val Met Gly Gly Arg Gly Phe Val Glu Val 
65                  70                  75                  80  


Asp Gly Lys Arg Phe Tyr Leu Glu Gln Phe His Phe His Ala Pro Ser 
                85                  90                  95      


Glu His Thr Val Asn Gly Lys His Phe Pro Phe Glu Ala His Phe Val 
            100                 105                 110         


His Leu Asp Glu Asn Gly Asn Ile Thr Val Leu Gly Val Phe Phe Glu 
        115                 120                 125             


Val Gly Lys Glu Asn Pro Glu Leu Asp Lys Val Trp Arg Val Met Pro 
    130                 135                 140                 


Asp Glu Pro Gly Gln Lys Arg His Leu Thr Ala Glu Ile Asp Pro Glu 
145                 150                 155                 160 


Lys Leu Leu Pro Glu Asn Arg Glu Tyr Phe Arg Tyr Ser Gly Ser Leu 
                165                 170                 175     


Thr Thr Pro Pro Cys Ser Glu Gly Val Arg Trp Ile Val Phe Lys Glu 
            180                 185                 190         


Pro Val Asp Met Ser Arg Asp Gln Leu Glu Lys Phe Arg Lys Val Met 
        195                 200                 205             


Gly Phe Asp Asn Asn Arg Pro Thr Gln Pro Ile Asn Ala Arg Lys Val 
    210                 215                 220                 


Met Arg 
225     


<210>  5
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amalgam of SEQ ID NOs: 2-4


<220>
<221>  VARIANT
<222>  (27)..(27)
<223>  Xaa can be K or R

<220>
<221>  VARIANT
<222>  (38)..(38)
<223>  Xaa can be N or D

<220>
<221>  VARIANT
<222>  (77)..(77)
<223>  Xaa can be Y or F

<220>
<221>  VARIANT
<222>  (79)..(79)
<223>  Xaa can be V or E

<220>
<221>  VARIANT
<222>  (88)..(88)
<223>  Xaa can be K, R, or E

<220>
<221>  VARIANT
<222>  (105)..(105)
<223>  Xaa can be Y or F

<220>
<221>  VARIANT
<222>  (116)..(116)
<223>  Xaa can be K, R, or E

<220>
<221>  VARIANT
<222>  (119)..(119)
<223>  Xaa can be N or D

<220>
<221>  VARIANT
<222>  (128)..(128)
<223>  Xaa can be K, R, or E

<220>
<221>  VARIANT
<222>  (137)..(137)
<223>  Xaa can be E or D

<220>
<221>  VARIANT
<222>  (145)..(145)
<223>  Xaa can be E or D

<220>
<221>  VARIANT
<222>  (156)..(156)
<223>  Xaa can be R or E

<220>
<221>  misc_feature
<222>  (160)..(160)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (168)..(168)
<223>  Xaa can be D or E

<220>
<221>  VARIANT
<222>  (170)..(170)
<223>  Xaa can be Y or F

<220>
<221>  VARIANT
<222>  (192)..(192)
<223>  Xaa can be E or D

<220>
<221>  VARIANT
<222>  (195)..(195)
<223>  Xaa can be E or D

<220>
<221>  VARIANT
<222>  (199)..(199)
<223>  Xaa can be E or D

<220>
<221>  VARIANT
<222>  (203)..(203)
<223>  Xaa can be K or R

<220>
<221>  VARIANT
<222>  (206)..(206)
<223>  Xaa can be K or R

<220>
<221>  VARIANT
<222>  (216)..(216)
<223>  Xaa can be V or T

<220>
<221>  VARIANT
<222>  (219)..(219)
<223>  Xaa can be L or I

<220>
<221>  VARIANT
<222>  (226)..(226)
<223>  Xaa can be K or R

<400>  5

Met Glu His Glu Trp Gly Tyr Ser Gly Ser Ile Gly Pro Glu His Trp 
1               5                   10                  15      


Gly Asp Leu Ser Pro Glu Tyr Leu Met Cys Xaa Ile Gly Lys Asn Gln 
            20                  25                  30          


Ser Pro Ile Asp Ile Xaa Ser Ala Asp Ala Val Lys Ala Cys Leu Ala 
        35                  40                  45              


Pro Val Ser Val Tyr Tyr Val Ser Asp Ala Lys Tyr Val Val Asn Asn 
    50                  55                  60                  


Gly His Thr Ile Lys Val Val Met Gly Gly Arg Gly Xaa Val Xaa Val 
65                  70                  75                  80  


Asp Gly Lys Arg Phe Tyr Leu Xaa Gln Phe His Phe His Ala Pro Ser 
                85                  90                  95      


Glu His Thr Val Asn Gly Lys His Xaa Pro Phe Glu Ala His Phe Val 
            100                 105                 110         


His Leu Asp Xaa Asn Gly Xaa Ile Thr Val Leu Gly Val Phe Phe Xaa 
        115                 120                 125             


Val Gly Lys Glu Asn Pro Glu Leu Xaa Lys Val Trp Arg Val Met Pro 
    130                 135                 140                 


Xaa Glu Pro Gly Gln Lys Arg His Leu Thr Ala Xaa Ile Asp Pro Xaa 
145                 150                 155                 160 


Lys Leu Leu Pro Glu Asn Arg Xaa Tyr Xaa Arg Tyr Ser Gly Ser Leu 
                165                 170                 175     


Thr Thr Pro Pro Cys Ser Glu Gly Val Arg Trp Ile Val Phe Lys Xaa 
            180                 185                 190         


Pro Val Xaa Met Ser Arg Xaa Gln Leu Glu Xaa Phe Arg Xaa Val Met 
        195                 200                 205             


Gly Phe Asp Asn Asn Arg Pro Xaa Gln Pro Xaa Asn Ala Arg Lys Val 
    210                 215                 220                 


Met Xaa 
225     


<210>  6
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 4 + S39I, E128K, T154D, K223I

<400>  6

Met Glu His Glu Trp Gly Tyr Ser Gly Ser Ile Gly Pro Glu His Trp 
1               5                   10                  15      


Gly Asp Leu Ser Pro Glu Tyr Leu Met Cys Lys Ile Gly Lys Asn Gln 
            20                  25                  30          


Ser Pro Ile Asp Ile Asn Ile Ala Asp Ala Val Lys Ala Cys Leu Ala 
        35                  40                  45              


Pro Val Ser Val Tyr Tyr Val Ser Asp Ala Lys Tyr Val Val Asn Asn 
    50                  55                  60                  


Gly His Thr Ile Lys Val Val Met Gly Gly Arg Gly Phe Val Glu Val 
65                  70                  75                  80  


Asp Gly Lys Arg Phe Tyr Leu Glu Gln Phe His Phe His Ala Pro Ser 
                85                  90                  95      


Glu His Thr Val Asn Gly Lys His Phe Pro Phe Glu Ala His Phe Val 
            100                 105                 110         


His Leu Asp Glu Asn Gly Asn Ile Thr Val Leu Gly Val Phe Phe Lys 
        115                 120                 125             


Val Gly Lys Glu Asn Pro Glu Leu Asp Lys Val Trp Arg Val Met Pro 
    130                 135                 140                 


Asp Glu Pro Gly Gln Lys Arg His Leu Asp Ala Glu Ile Asp Pro Glu 
145                 150                 155                 160 


Lys Leu Leu Pro Glu Asn Arg Glu Tyr Phe Arg Tyr Ser Gly Ser Leu 
                165                 170                 175     


Thr Thr Pro Pro Cys Ser Glu Gly Val Arg Trp Ile Val Phe Lys Glu 
            180                 185                 190         


Pro Val Glu Met Ser Arg Asp Gln Leu Glu Lys Phe Arg Lys Val Met 
        195                 200                 205             


Gly Phe Asp Asn Asn Arg Pro Thr Gln Pro Ile Asn Ala Arg Ile Val 
    210                 215                 220                 


Met Arg 
225     


<210>  7
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 4 + S39I, G130A, T154D, K223I

<400>  7

Met Glu His Glu Trp Gly Tyr Ser Gly Ser Ile Gly Pro Glu His Trp 
1               5                   10                  15      


Gly Asp Leu Ser Pro Glu Tyr Leu Met Cys Lys Ile Gly Lys Asn Gln 
            20                  25                  30          


Ser Pro Ile Asp Ile Asn Ile Ala Asp Ala Val Lys Ala Cys Leu Ala 
        35                  40                  45              


Pro Val Ser Val Tyr Tyr Val Ser Asp Ala Lys Tyr Val Val Asn Asn 
    50                  55                  60                  


Gly His Thr Ile Lys Val Val Met Gly Gly Arg Gly Phe Val Glu Val 
65                  70                  75                  80  


Asp Gly Lys Arg Phe Tyr Leu Glu Gln Phe His Phe His Ala Pro Ser 
                85                  90                  95      


Glu His Thr Val Asn Gly Lys His Phe Pro Phe Glu Ala His Phe Val 
            100                 105                 110         


His Leu Asp Glu Asn Gly Asn Ile Thr Val Leu Gly Val Phe Phe Glu 
        115                 120                 125             


Val Ala Lys Glu Asn Pro Glu Leu Asp Lys Val Trp Arg Val Met Pro 
    130                 135                 140                 


Asp Glu Pro Gly Gln Lys Arg His Leu Asp Ala Glu Ile Asp Pro Glu 
145                 150                 155                 160 


Lys Leu Leu Pro Glu Asn Arg Glu Tyr Phe Arg Tyr Ser Gly Ser Leu 
                165                 170                 175     


Thr Thr Pro Pro Cys Ser Glu Gly Val Arg Trp Ile Val Phe Lys Glu 
            180                 185                 190         


Pro Val Glu Met Ser Arg Asp Gln Leu Glu Lys Phe Arg Lys Val Met 
        195                 200                 205             


Gly Phe Asp Asn Asn Arg Pro Thr Gln Pro Ile Asn Ala Arg Ile Val 
    210                 215                 220                 


Met Arg 
225     


<210>  8
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 4 + S39I, G130A, T154D, K223L

<400>  8

Met Glu His Glu Trp Gly Tyr Ser Gly Ser Ile Gly Pro Glu His Trp 
1               5                   10                  15      


Gly Asp Leu Ser Pro Glu Tyr Leu Met Cys Lys Ile Gly Lys Asn Gln 
            20                  25                  30          


Ser Pro Ile Asp Ile Asn Ile Ala Asp Ala Val Lys Ala Cys Leu Ala 
        35                  40                  45              


Pro Val Ser Val Tyr Tyr Val Ser Asp Ala Lys Tyr Val Val Asn Asn 
    50                  55                  60                  


Gly His Thr Ile Lys Val Val Met Gly Gly Arg Gly Phe Val Glu Val 
65                  70                  75                  80  


Asp Gly Lys Arg Phe Tyr Leu Glu Gln Phe His Phe His Ala Pro Ser 
                85                  90                  95      


Glu His Thr Val Asn Gly Lys His Phe Pro Phe Glu Ala His Phe Val 
            100                 105                 110         


His Leu Asp Glu Asn Gly Asn Ile Thr Val Leu Gly Val Phe Phe Glu 
        115                 120                 125             


Val Ala Lys Glu Asn Pro Glu Leu Asp Lys Val Trp Arg Val Met Pro 
    130                 135                 140                 


Asp Glu Pro Gly Gln Lys Arg His Leu Asp Ala Glu Ile Asp Pro Glu 
145                 150                 155                 160 


Lys Leu Leu Pro Glu Asn Arg Glu Tyr Phe Arg Tyr Ser Gly Ser Leu 
                165                 170                 175     


Thr Thr Pro Pro Cys Ser Glu Gly Val Arg Trp Ile Val Phe Lys Glu 
            180                 185                 190         


Pro Val Glu Met Ser Arg Asp Gln Leu Glu Lys Phe Arg Lys Val Met 
        195                 200                 205             


Gly Phe Asp Asn Asn Arg Pro Thr Gln Pro Ile Asn Ala Arg Leu Val 
    210                 215                 220                 


Met Arg 
225     


<210>  9
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 4 + S39I, G130A, T154P, D195E, K223I

<400>  9

Met Glu His Glu Trp Gly Tyr Ser Gly Ser Ile Gly Pro Glu His Trp 
1               5                   10                  15      


Gly Asp Leu Ser Pro Glu Tyr Leu Met Cys Lys Ile Gly Lys Asn Gln 
            20                  25                  30          


Ser Pro Ile Asp Ile Asn Ile Ala Asp Ala Val Lys Ala Cys Leu Ala 
        35                  40                  45              


Pro Val Ser Val Tyr Tyr Val Ser Asp Ala Lys Tyr Val Val Asn Asn 
    50                  55                  60                  


Gly His Thr Ile Lys Val Val Met Gly Gly Arg Gly Phe Val Glu Val 
65                  70                  75                  80  


Asp Gly Lys Arg Phe Tyr Leu Glu Gln Phe His Phe His Ala Pro Ser 
                85                  90                  95      


Glu His Thr Val Asn Gly Lys His Phe Pro Phe Glu Ala His Phe Val 
            100                 105                 110         


His Leu Asp Glu Asn Gly Asn Ile Thr Val Leu Gly Val Phe Phe Glu 
        115                 120                 125             


Val Ala Lys Glu Asn Pro Glu Leu Asp Lys Val Trp Arg Val Met Pro 
    130                 135                 140                 


Asp Glu Pro Gly Gln Lys Arg His Leu Pro Ala Glu Ile Asp Pro Glu 
145                 150                 155                 160 


Lys Leu Leu Pro Glu Asn Arg Glu Tyr Phe Arg Tyr Ser Gly Ser Leu 
                165                 170                 175     


Thr Thr Pro Pro Cys Ser Glu Gly Val Arg Trp Ile Val Phe Lys Glu 
            180                 185                 190         


Pro Val Glu Met Ser Arg Asp Gln Leu Glu Lys Phe Arg Lys Val Met 
        195                 200                 205             


Gly Phe Asp Asn Asn Arg Pro Thr Gln Pro Ile Asn Ala Arg Ile Val 
    210                 215                 220                 


Met Arg 
225     


<210>  10
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 4 + S39I, G130A, T154P, D195E, K223L

<400>  10

Met Glu His Glu Trp Gly Tyr Ser Gly Ser Ile Gly Pro Glu His Trp 
1               5                   10                  15      


Gly Asp Leu Ser Pro Glu Tyr Leu Met Cys Lys Ile Gly Lys Asn Gln 
            20                  25                  30          


Ser Pro Ile Asp Ile Asn Ile Ala Asp Ala Val Lys Ala Cys Leu Ala 
        35                  40                  45              


Pro Val Ser Val Tyr Tyr Val Ser Asp Ala Lys Tyr Val Val Asn Asn 
    50                  55                  60                  


Gly His Thr Ile Lys Val Val Met Gly Gly Arg Gly Phe Val Glu Val 
65                  70                  75                  80  


Asp Gly Lys Arg Phe Tyr Leu Glu Gln Phe His Phe His Ala Pro Ser 
                85                  90                  95      


Glu His Thr Val Asn Gly Lys His Phe Pro Phe Glu Ala His Phe Val 
            100                 105                 110         


His Leu Asp Glu Asn Gly Asn Ile Thr Val Leu Gly Val Phe Phe Glu 
        115                 120                 125             


Val Ala Lys Glu Asn Pro Glu Leu Asp Lys Val Trp Arg Val Met Pro 
    130                 135                 140                 


Asp Glu Pro Gly Gln Lys Arg His Leu Pro Ala Glu Ile Asp Pro Glu 
145                 150                 155                 160 


Lys Leu Leu Pro Glu Asn Arg Glu Tyr Phe Arg Tyr Ser Gly Ser Leu 
                165                 170                 175     


Thr Thr Pro Pro Cys Ser Glu Gly Val Arg Trp Ile Val Phe Lys Glu 
            180                 185                 190         


Pro Val Glu Met Ser Arg Asp Gln Leu Glu Lys Phe Arg Lys Val Met 
        195                 200                 205             


Gly Phe Asp Asn Asn Arg Pro Thr Gln Pro Ile Asn Ala Arg Leu Val 
    210                 215                 220                 


Met Arg 
225     


<210>  11
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 4 + S39I, K223L

<400>  11

Met Glu His Glu Trp Gly Tyr Ser Gly Ser Ile Gly Pro Glu His Trp 
1               5                   10                  15      


Gly Asp Leu Ser Pro Glu Tyr Leu Met Cys Lys Ile Gly Lys Asn Gln 
            20                  25                  30          


Ser Pro Ile Asp Ile Asn Ile Ala Asp Ala Val Lys Ala Cys Leu Ala 
        35                  40                  45              


Pro Val Ser Val Tyr Tyr Val Ser Asp Ala Lys Tyr Val Val Asn Asn 
    50                  55                  60                  


Gly His Thr Ile Lys Val Val Met Gly Gly Arg Gly Phe Val Glu Val 
65                  70                  75                  80  


Asp Gly Lys Arg Phe Tyr Leu Glu Gln Phe His Phe His Ala Pro Ser 
                85                  90                  95      


Glu His Thr Val Asn Gly Lys His Phe Pro Phe Glu Ala His Phe Val 
            100                 105                 110         


His Leu Asp Glu Asn Gly Asn Ile Thr Val Leu Gly Val Phe Phe Glu 
        115                 120                 125             


Val Gly Lys Glu Asn Pro Glu Leu Asp Lys Val Trp Arg Val Met Pro 
    130                 135                 140                 


Asp Glu Pro Gly Gln Lys Arg His Leu Thr Ala Glu Ile Asp Pro Glu 
145                 150                 155                 160 


Lys Leu Leu Pro Glu Asn Arg Glu Tyr Phe Arg Tyr Ser Gly Ser Leu 
                165                 170                 175     


Thr Thr Pro Pro Cys Ser Glu Gly Val Arg Trp Ile Val Phe Lys Glu 
            180                 185                 190         


Pro Val Asp Met Ser Arg Asp Gln Leu Glu Lys Phe Arg Lys Val Met 
        195                 200                 205             


Gly Phe Asp Asn Asn Arg Pro Thr Gln Pro Ile Asn Ala Arg Leu Val 
    210                 215                 220                 


Met Arg 
225     


<210>  12
<211>  243
<212>  PRT
<213>  Persephonella marina (WP_015898908.1)

<400>  12

Met Lys Lys Phe Val Val Gly Leu Ser Ser Leu Val Leu Ala Thr Ser 
1               5                   10                  15      


Ser Phe Ala Gly Gly Gly Trp Ser Tyr His Gly Glu His Gly Pro Glu 
            20                  25                  30          


His Trp Gly Asp Leu Lys Asp Glu Tyr Ile Met Cys Lys Ile Gly Lys 
        35                  40                  45              


Asn Gln Ser Pro Val Asp Ile Asn Arg Ile Val Asp Ala Lys Leu Lys 
    50                  55                  60                  


Pro Ile Lys Ile Glu Tyr Arg Ala Gly Ala Thr Lys Val Leu Asn Asn 
65                  70                  75                  80  


Gly His Thr Ile Lys Val Ser Tyr Glu Pro Gly Ser Tyr Ile Val Val 
                85                  90                  95      


Asp Gly Ile Lys Phe Glu Leu Lys Gln Phe His Phe His Ala Pro Ser 
            100                 105                 110         


Glu His Lys Leu Lys Gly Gln His Tyr Pro Phe Glu Ala His Phe Val 
        115                 120                 125             


His Ala Asp Lys His Gly Asn Leu Ala Val Ile Gly Val Phe Phe Lys 
    130                 135                 140                 


Glu Gly Arg Glu Asn Pro Ile Leu Glu Lys Ile Trp Lys Val Met Pro 
145                 150                 155                 160 


Glu Asn Ala Gly Glu Glu Val Lys Leu Ala His Lys Ile Asn Ala Glu 
                165                 170                 175     


Asp Leu Leu Pro Lys Asp Arg Asp Tyr Tyr Arg Tyr Ser Gly Ser Leu 
            180                 185                 190         


Thr Thr Pro Pro Cys Ser Glu Gly Val Arg Trp Ile Val Met Glu Glu 
        195                 200                 205             


Glu Met Glu Met Ser Lys Glu Gln Ile Glu Lys Phe Arg Lys Ile Met 
    210                 215                 220                 


Gly Gly Asp Thr Asn Arg Pro Val Gln Pro Leu Asn Ala Arg Met Ile 
225                 230                 235                 240 


Met Glu Lys 
            


<210>  13
<211>  246
<212>  PRT
<213>  Persephonella sp. KM09-Lau-8 (WP_029522463.1)

<400>  13

Met Arg Lys Thr Thr Phe Val Ala Gly Leu Ser Phe Leu Ile Met Gly 
1               5                   10                  15      


Tyr Gly Ala Tyr Ser Gly Glu His Trp Ser Tyr His Gly Lys Thr Gly 
            20                  25                  30          


Pro Glu His Trp Gly Asp Leu Lys Pro Glu Phe Ile Met Cys Lys Ile 
        35                  40                  45              


Gly Lys Asn Gln Ser Pro Val Asp Ile Asn Arg Ile Val Glu Ala Glu 
    50                  55                  60                  


Leu Lys Glu Leu Lys Ile Ser Tyr Arg Pro Gly Ala Ser Tyr Leu Val 
65                  70                  75                  80  


Asn Asn Gly His Thr Ile Lys Val Ser Tyr Gly Lys Gly Ser Tyr Leu 
                85                  90                  95      


Glu Val Asp Gly Thr Lys Phe Glu Leu Lys Gln Phe His Phe His Ala 
            100                 105                 110         


Pro Ser Glu His Lys Ile Lys Gly Lys Leu Tyr Pro Phe Glu Ala His 
        115                 120                 125             


Phe Val His Met Asp Lys Asn Gly Asn Ile Ala Val Ile Gly Val Leu 
    130                 135                 140                 


Phe Lys Glu Gly Arg Lys Asn Pro Val Ile Glu Lys Leu Trp Lys Asn 
145                 150                 155                 160 


Ile Ser Ser Lys Val Gly Glu Lys Val Lys Ile Thr Asp Lys Ile Asn 
                165                 170                 175     


Ala Tyr Asp Leu Leu Pro Lys Lys Lys Arg Tyr Tyr Arg Tyr Ser Gly 
            180                 185                 190         


Ser Leu Thr Thr Pro Pro Cys Ser Glu Gly Val Arg Trp Phe Val Met 
        195                 200                 205             


Val Glu Glu Met Glu Leu Ser Lys Glu Gln Ile Glu Lys Phe Arg Lys 
    210                 215                 220                 


Leu Met Gly Gly Asp Thr Asn Arg Pro Val Gln Pro Leu Asn Ala Arg 
225                 230                 235                 240 


Met Val Met Lys Gln Asp 
                245     


<210>  14
<211>  247
<212>  PRT
<213>  Persephonella sp. IF05-L8 (WP_029521561.1)

<400>  14

Met Lys Lys Lys Leu Leu Ser Val Ser Ile Ala Ser Ala Val Phe Ser 
1               5                   10                  15      


Phe Ala Phe Ala Gly Gly Asn Gly Asp Trp Ser Tyr His Gly Ser Thr 
            20                  25                  30          


Gly Pro Glu His Trp Gly Asp Leu Lys Glu Glu Tyr Ile Met Cys Lys 
        35                  40                  45              


Ile Gly Lys Asn Gln Ser Pro Val Asp Ile Asn Arg Ile Val Glu Ala 
    50                  55                  60                  


Glu Leu Glu Glu Ile Lys Ile Ser Tyr Lys Pro Gly Ala Ser Ser Leu 
65                  70                  75                  80  


Val Asn Asn Gly His Thr Ile Lys Val Ser Tyr Glu Gly Gly Ser Tyr 
                85                  90                  95      


Ile Val Ile Asp Gly Ile Lys Phe Glu Leu Lys Gln Phe His Phe His 
            100                 105                 110         


Ala Pro Ser Glu His Lys Ile Lys Gly Lys Leu Tyr Pro Phe Glu Ala 
        115                 120                 125             


His Phe Val His Ala Asp Lys Asp Gly Asn Leu Ala Val Ile Gly Val 
    130                 135                 140                 


Val Phe Lys Glu Gly Lys Lys Asn Pro Val Ile Glu Lys Leu Trp Lys 
145                 150                 155                 160 


Asn Leu Pro Ser Lys Val Gly Glu Glu Val Lys Leu Ser Glu Lys Ile 
                165                 170                 175     


Asn Ala Tyr Asp Leu Leu Pro Lys Lys Lys Lys Tyr Phe Arg Tyr Ser 
            180                 185                 190         


Gly Ser Leu Thr Thr Pro Pro Cys Ser Glu Gly Val Arg Trp Ile Val 
        195                 200                 205             


Met Thr Lys Glu Met Glu Leu Ser Lys Glu Gln Ile Glu Lys Phe Arg 
    210                 215                 220                 


Lys Leu Met Gly Gly Asp Thr Asn Arg Pro Val Gln Pro Leu Asn Ala 
225                 230                 235                 240 


Arg Met Ile Met Ile Glu Asp 
                245         


<210>  15
<211>  244
<212>  PRT
<213>  uncultured bacterium (AVN84966.1)

<400>  15

Met Lys Lys Leu Ala Phe Ile Ile Phe Ser Leu Thr Ile Gly Thr Val 
1               5                   10                  15      


Ile Ala Gly Gly Val Gly His Trp Ser Tyr His Gly Glu Thr Gly Pro 
            20                  25                  30          


Gln His Trp Gly Asp Leu Lys Asn Glu Tyr Ile Met Cys Lys Ile Gly 
        35                  40                  45              


Lys Asn Gln Ser Pro Val Asp Ile Ser Arg Ile Val Glu Ala Glu Leu 
    50                  55                  60                  


Glu Lys Ile Lys Ile Asn Tyr Ser Ser Gly Gly Ser Ser Ile Thr Asn 
65                  70                  75                  80  


Asn Gly His Thr Ile Lys Val Ser Tyr Glu Pro Gly Ser Tyr Ile Ile 
                85                  90                  95      


Val Asp Gly Ile Arg Phe Glu Leu Lys Gln Phe His Phe His Ala Pro 
            100                 105                 110         


Ser Glu His Thr Ile Lys Gly Lys Ser Tyr Pro Phe Glu Ala His Phe 
        115                 120                 125             


Val His Ala Asp Lys Asp Gly Asn Leu Ala Val Ile Gly Val Ile Phe 
    130                 135                 140                 


Lys Glu Gly Lys Lys Asn Pro Ile Ile Glu Lys Ile Trp Glu Asn Leu 
145                 150                 155                 160 


Pro Glu Ala Gly Lys Thr Ile Lys Leu Ala His Lys Ile Asn Ala Tyr 
                165                 170                 175     


Asp Leu Leu Pro Lys Lys Lys Lys Tyr Tyr Arg Tyr Ser Gly Ser Leu 
            180                 185                 190         


Thr Thr Pro Pro Cys Ser Glu Gly Val Arg Trp Ile Val Met Glu Glu 
        195                 200                 205             


Glu Met Glu Leu Ser Lys Glu Gln Ile Glu Lys Phe Arg Lys Leu Met 
    210                 215                 220                 


Gly Gly Asp Thr Asn Arg Pro Val Gln Pro Leu Asn Ala Arg Met Ile 
225                 230                 235                 240 


Met Glu Met Asp 
                


<210>  16
<211>  247
<212>  PRT
<213>  Persephonella hydrogeniphila (WP_096999253.1)

<400>  16

Met Lys Lys Ala Val Leu Ser Val Ala Thr Ala Ser Ala Ile Phe Gly 
1               5                   10                  15      


Ile Ala Val Ala Gly Gly Ala Gly Ser Trp Ser Tyr His Gly Ala Thr 
            20                  25                  30          


Gly Pro Glu His Trp Gly Asp Leu Lys Asp Glu Tyr Ile Met Cys Lys 
        35                  40                  45              


Ile Gly Lys Asn Gln Ser Pro Val Asp Ile Ser Arg Ile Ala Glu Ala 
    50                  55                  60                  


Asp Leu Glu Asp Ile Lys Phe His Tyr Ser Pro Gly Ala Thr Ser Leu 
65                  70                  75                  80  


Leu Asn Asn Gly His Thr Ile Lys Val Ser Tyr Glu Pro Gly Ser Tyr 
                85                  90                  95      


Ile Val Val Asp Gly Ile Arg Phe Glu Leu Lys Gln Phe His Phe His 
            100                 105                 110         


Ala Pro Ser Glu His Thr Val Lys Gly Lys Ser Tyr Pro Phe Glu Ala 
        115                 120                 125             


His Phe Val His Ala Asp Lys Asp Gly Asn Leu Ala Val Ile Gly Val 
    130                 135                 140                 


Leu Phe Lys Glu Gly Lys Glu Asn Pro Val Ile Lys Lys Leu Trp Glu 
145                 150                 155                 160 


Asn Leu Pro Ser His Thr Gly Glu Lys Val Lys Leu Ala His Lys Ile 
                165                 170                 175     


Asn Ala Tyr Asp Leu Leu Pro Lys Arg Lys Lys Tyr Tyr Arg Tyr Ser 
            180                 185                 190         


Gly Ser Leu Thr Thr Pro Pro Cys Ser Glu Gly Val Arg Trp Leu Val 
        195                 200                 205             


Met Thr Thr Glu Met Glu Met Ser Lys Glu Gln Ile Glu Lys Phe Arg 
    210                 215                 220                 


Lys Leu Met Gly Gly Asp Thr Asn Arg Pro Val Gln Pro Val Asn Ala 
225                 230                 235                 240 


Arg Met Ile Leu Ile Lys Asp 
                245         


<210>  17
<211>  245
<212>  PRT
<213>  Aquificae bacterium (RMD45622.1)

<400>  17

Met Arg Lys Lys Val Leu Gly Leu Ile Leu Ala Leu Ser Phe Ser Asn 
1               5                   10                  15      


Tyr Ser Ile Ala Gly Glu Trp Ser Tyr His Gly Glu His Gly Pro Glu 
            20                  25                  30          


His Trp Gly Asp Ile Lys Lys Glu Phe Ile Met Cys Lys Ile Gly Lys 
        35                  40                  45              


Asn Gln Ser Pro Val Asp Leu Asn Arg Ile Val Lys Ala Glu Leu Lys 
    50                  55                  60                  


Glu Val Asp Ile Asn Tyr Lys Pro Gly Gly Ile Ser Ala Leu Asn Asn 
65                  70                  75                  80  


Gly His Thr Ile Lys Val Ser Tyr Glu Pro Gly Ser Tyr Ile Val Ile 
                85                  90                  95      


Asp Gly Ile Lys Phe Glu Leu Lys Gln Phe His Phe His Ser Pro Ser 
            100                 105                 110         


Glu His Lys Ile Lys Gly Lys Gln Tyr Pro Leu Glu Ala His Phe Val 
        115                 120                 125             


His Ala Asp Lys Asp Gly Asn Leu Ala Val Ile Gly Val Val Phe Lys 
    130                 135                 140                 


Glu Gly Lys Glu Asn Pro Ile Ile Lys Gln Ile Trp Glu Asn Leu Pro 
145                 150                 155                 160 


Met Glu Glu Gly Lys Glu Val Lys Leu Ser Gln Lys Val Asn Ala Tyr 
                165                 170                 175     


Glu Leu Leu Pro Glu Asp Lys Asn Tyr Tyr Arg Tyr Ser Gly Ser Leu 
            180                 185                 190         


Thr Thr Pro Pro Cys Ser Glu Gly Val Arg Trp Ile Val Met Glu Lys 
        195                 200                 205             


Pro Leu Glu Val Ser Lys Glu Gln Ile Glu Lys Phe Arg Lys Ala Met 
    210                 215                 220                 


Gly Asn Val Asp Thr Asn Arg Pro Val Gln Pro Leu Asn Ala Arg Leu 
225                 230                 235                 240 


Ile Leu Glu Lys Glu 
                245 


<210>  18
<211>  249
<212>  PRT
<213>  Caminibacter mediatlanticus (WP_007474387.1)

<400>  18

Met Lys Lys Phe Leu Leu Gly Leu Gly Val Ala Ala Ile Thr Met Leu 
1               5                   10                  15      


Ser Ala Ser Ser Tyr Asn Tyr His Ala Thr Trp Ser Tyr Ser Gly Lys 
            20                  25                  30          


Thr Gly Pro Glu Tyr Trp Gly Asp Leu Lys Lys Glu Tyr Gln Met Cys 
        35                  40                  45              


Lys Ile Gly Lys Asn Gln Ser Pro Ile Asp Ile Lys Thr Asn Ser Thr 
    50                  55                  60                  


Gln Thr Phe Asn Thr Asn Leu Lys Pro Phe Lys Ile Lys Tyr Leu Gly 
65                  70                  75                  80  


Lys Gly Tyr Glu Val Ile Asn Asn Gly His Thr Ile Lys Val Lys Thr 
                85                  90                  95      


Glu Gly Lys Asn Cys Val Arg Ile Asp Gly Ile Lys Phe Lys Leu Ala 
            100                 105                 110         


Gln Leu His Phe His Thr Pro Ser Glu Asn Thr Ile Asn Gly Lys His 
        115                 120                 125             


Phe Pro Met Glu Ala His Tyr Val His Leu Asp Lys Asn Gly Asn Ile 
    130                 135                 140                 


Thr Val Leu Ala Val Met Tyr Lys Ile Gly Lys Glu Asn Lys Ser Leu 
145                 150                 155                 160 


Asn Lys Met Leu Ala Val Leu Pro Thr Lys Val Gly Glu Glu Asn Lys 
                165                 170                 175     


Val Met Gly Asn Leu Asn Pro Met Glu Leu Leu Pro Lys Asn Lys Ala 
            180                 185                 190         


Tyr Tyr Arg Phe Asn Gly Ser Leu Thr Thr Pro Pro Cys Ser Glu Gly 
        195                 200                 205             


Val Arg Trp Ile Val Phe Lys Thr Pro Val Glu Ile Ser Gln Ala Gln 
    210                 215                 220                 


Tyr Glu Lys Met His Ala Val Met Gly Thr Asn Asn Arg Pro Val Gln 
225                 230                 235                 240 


Pro Ile Asn Ala Arg Val Ile Leu Lys 
                245                 


<210>  19
<211>  248
<212>  PRT
<213>  Hydrogenimonas sp. (BBG65557.1)

<400>  19

Met Lys Lys Ile Thr Ser Leu Phe Leu Gly Ala Ala Thr Ala Ser Val 
1               5                   10                  15      


Leu Met Ala Gly Gly His Gly Val Glu Trp Gly Tyr Thr Gly His Thr 
            20                  25                  30          


Gly Pro Glu His Trp Gly Asp Leu Ser Asp Lys Tyr Ile Met Cys Lys 
        35                  40                  45              


Leu Gly Lys Asn Gln Ser Pro Ile Asp Ile Lys Thr Ala Asp Ala Phe 
    50                  55                  60                  


Glu Thr Asp Leu Pro Glu Ile Ala Ile Asp Tyr Lys Ser Ala Ser Val 
65                  70                  75                  80  


Asn Glu Val Asn Asn Gly His Thr Val Gln Val Asn Ile Val Pro Gly 
                85                  90                  95      


Ser Ser Ile Thr Val Asp Gly Ile Lys Phe Glu Leu Lys Gln Phe His 
            100                 105                 110         


Phe His Thr Pro Ser Glu Asn Thr Ile Asp Gly Arg Tyr Phe Pro Leu 
        115                 120                 125             


Glu Ala His Phe Val His Ala Asp Glu Asn Gly Asn Leu Ala Val Ile 
    130                 135                 140                 


Gly Val Met Phe Glu Tyr Gly Lys Glu Asn Pro Val Leu Lys Ser Val 
145                 150                 155                 160 


Trp Glu Arg Met Pro Glu His Ala Lys Asp Val Glu Lys Ile Thr Leu 
                165                 170                 175     


Ser Ala Glu Glu Val Lys Asn Leu Leu Pro Ala Asn Lys Glu Tyr Tyr 
            180                 185                 190         


Arg Phe Asn Gly Ser Leu Thr Thr Pro Pro Cys Ser Glu Gly Val Arg 
        195                 200                 205             


Trp Phe Val Met Lys Glu Pro Val Thr Val Ser Lys Asp Gln Val Lys 
    210                 215                 220                 


Lys Phe Ala Glu Val Met His His Asp Asn Asn Arg Pro Val Gln Pro 
225                 230                 235                 240 


Val Asn Ala Arg Ile Ile Leu Lys 
                245             


<210>  20
<211>  249
<212>  PRT
<213>  Hydrogenimonas sp. (RUM45284.1)

<400>  20

Met Lys Lys Val Val Ser Leu Leu Ile Gly Ser Ala Val Ala Ala Thr 
1               5                   10                  15      


Leu Val Met Ala Gln Glu His Thr Ile His Trp Gly Tyr Ser Gly His 
            20                  25                  30          


Gly Gly Pro Glu His Trp Gly Glu Leu Ser Lys Asp Tyr Gln Met Cys 
        35                  40                  45              


Leu Ile Gly Lys Asn Gln Ser Pro Ile Asp Ile Arg Thr Lys Ser Ala 
    50                  55                  60                  


Phe Glu Thr Lys Leu Pro Ala Ile Gln Phe Asp Tyr His Thr Ser Ala 
65                  70                  75                  80  


Lys Asp Val Val Asp Asn Gly His Thr Glu Gln Val Asp Leu Val Pro 
                85                  90                  95      


Gly Ser Ser Ile Thr Ile Asp Gly Arg Lys Phe Glu Leu Lys Gln Phe 
            100                 105                 110         


His Phe His Thr Pro Ser Glu Asn Arg Ile Asp Gly Lys Ser Phe Pro 
        115                 120                 125             


Leu Glu Ala His Phe Val His Ala Asp Glu His Gly Asn Leu Ala Val 
    130                 135                 140                 


Val Ala Leu Met Phe Thr Tyr Gly Glu Ala Asn Pro Val Leu Ala Lys 
145                 150                 155                 160 


Val Trp Glu Asn Met Pro Glu His Ala Gly Glu Lys His His Phe Thr 
                165                 170                 175     


Leu Ser Ser Glu Asp Val Met Lys Leu Leu Pro Lys Asn Arg Asp Tyr 
            180                 185                 190         


Tyr Arg Phe Asn Gly Ser Leu Thr Thr Pro Pro Cys Thr Glu Gly Val 
        195                 200                 205             


Arg Trp Phe Val Ile Lys Glu Pro Val Thr Ile Ser Lys Glu Gln Val 
    210                 215                 220                 


Gln Lys Phe Ala Lys Ile Met His His Asn Asn Asn Arg Pro Val Gln 
225                 230                 235                 240 


Pro Leu Asn Ser Arg Met Ile Leu Lys 
                245                 


